151. Neurite outgrowth in PC12 cells is enhanced by guanosine through both cAMP-dependent and -independent mechanisms.
- Author
-
Gysbers JW and Rathbone MP
- Subjects
- Adenine analogs & derivatives, Adenine pharmacology, Adenosine analogs & derivatives, Adenosine pharmacology, Adenosine-5'-(N-ethylcarboxamide), Adenylyl Cyclase Inhibitors, Animals, Antineoplastic Agents pharmacology, Colforsin pharmacology, Enzyme Inhibitors pharmacology, Guanosine Triphosphate physiology, Nerve Growth Factors pharmacology, Neurites drug effects, Neurites ultrastructure, PC12 Cells, Rats, Cyclic AMP physiology, Guanosine pharmacology, Neurites physiology
- Abstract
Extracellular guanosine, guanosine triphosphate (GTP), and 5'-N'-ethylcarboxamidoadenosine (NECA), each significantly enhanced the proportion of nerve growth factor (NGF)-treated rat pheochromocytoma (PC12) cells which had neurites, greater than that in cultures exposed to NGF alone. Guanosine and NECA, but not GTP, increased intracellular cAMP concentrations. An adenylate cyclase inhibitor, SQ22536, completely blocked the cAMP increase induced by both guanosine and 0.1 microM NECA. However, SQ22536 only partially blocked guanosine enhanced neurite outgrowth, although it completely blocked the neuritogenic effect of NECA. Therefore guanosine-enhanced neurite outgrowth through both cAMP-dependent and -independent mechanisms, while the effect of GTP was cAMP-independent.
- Published
- 1996
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