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Neurite outgrowth in PC12 cells is enhanced by guanosine through both cAMP-dependent and -independent mechanisms.
- Source :
-
Neuroscience letters [Neurosci Lett] 1996 Dec 20; Vol. 220 (3), pp. 175-8. - Publication Year :
- 1996
-
Abstract
- Extracellular guanosine, guanosine triphosphate (GTP), and 5'-N'-ethylcarboxamidoadenosine (NECA), each significantly enhanced the proportion of nerve growth factor (NGF)-treated rat pheochromocytoma (PC12) cells which had neurites, greater than that in cultures exposed to NGF alone. Guanosine and NECA, but not GTP, increased intracellular cAMP concentrations. An adenylate cyclase inhibitor, SQ22536, completely blocked the cAMP increase induced by both guanosine and 0.1 microM NECA. However, SQ22536 only partially blocked guanosine enhanced neurite outgrowth, although it completely blocked the neuritogenic effect of NECA. Therefore guanosine-enhanced neurite outgrowth through both cAMP-dependent and -independent mechanisms, while the effect of GTP was cAMP-independent.
- Subjects :
- Adenine analogs & derivatives
Adenine pharmacology
Adenosine analogs & derivatives
Adenosine pharmacology
Adenosine-5'-(N-ethylcarboxamide)
Adenylyl Cyclase Inhibitors
Animals
Antineoplastic Agents pharmacology
Colforsin pharmacology
Enzyme Inhibitors pharmacology
Guanosine Triphosphate physiology
Nerve Growth Factors pharmacology
Neurites drug effects
Neurites ultrastructure
PC12 Cells
Rats
Cyclic AMP physiology
Guanosine pharmacology
Neurites physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0304-3940
- Volume :
- 220
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Neuroscience letters
- Publication Type :
- Academic Journal
- Accession number :
- 8994221
- Full Text :
- https://doi.org/10.1016/s0304-3940(96)13253-5