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Sanbao large yellow croaker is a kind of Chinese salted and fermented seafood processed by adding 50% of salt and fermenting at high temperature [around 30 °C] over 20 d. To get a comprehensive understanding of cultivable microorganism's diversity present in its fermentation, the chemical and microbial properties of this product, were detected initially, followed by identification of bacteria recovered from different fermentation periods using PCR-RFLP and 16S rRNA gene sequencing analysis. In total, 105 indigenous isolates were recovered with 3 different medium and majority of the isolates picked up from both MRS and MSA medium were finally clustered into the genus of Staphylococcus ( S.). Among the 90 Staphylococci, S. xylosus, S. saprophyticus, and S. nepalensis were the most prevailing cultivable species recovered throughout the whole production process (70 isolates, 77.8%), while 5 other species, namely, S. aureus, S. vitulinus, S. sciuri, S. equorum, and S. succinus formed a minor fraction (20 isolates, 22.2%) of the Staphylococcus communities. Lactic acid bacteria, Pseudomonas, Proteus, and Bacillus constituted trivial populations in the initial period of the fermentation and then gave the way to the Staphylococcus immediately. High salt concentration used during the processing is like to have a pronounced influence on the microbial populations involved. Data obtained in this work could be referred in control and optimization of the fermentation process and selecting suitable strains for aquatic product fermentation. [ABSTRACT FROM AUTHOR]

Chouguiyu, or stinky mandarinfish, is a traditional Chinese fermented fish product made of mandarinfish by spontaneous fermentation at the anaerobic condition with low-salt concentration. In order to get a primary understanding of the microbial community presenting in the Chouguiyu fermentation, 61 cultures of lactic acid bacteria (LAB) from various fermentation period were isolated using MRS agar plates and characterized based on a combination of phenotypic and genotypic approaches including amplified ribosomal DNA restriction analysis (ARDRA) and 16S rRNA partial gene sequencing analysis. Eight distinct bacterial species belonging to 6 genera were identified in total. Among them, Lactobacillus sakei was the dominant species (63%) during the fermentation, which exhibited great variety in phenotypic tests but unique genotypic characters. Meanwhile, the other LAB species including Lactococcus ( Lc.) garvieae, Lc. lactis, Lc. raffinolactis, Vagococcus sp., Enterococcus hermanniensis, Macrococcus caseolyticus as well as Streptococcus parauberis were also recovered from the different fermentation periods, especially at the initial point of the fermentation. This seems to be the 1st report investigating the LAB composition involved in Chouguiyu fermentation and the data obtained in this study may be valuable for selecting starter culture for Chouguiyu industrial-scale production. [ABSTRACT FROM AUTHOR]

Inverted NiOx‐based perovskite solar cells (PSCs) exhibit considerable potential because of their low‐temperature processing and outstanding excellent stability, while is challenged by the carriers transfer at buried interface owing to the inherent low carrier mobility and abundant surface defects that directly deteriorates the overall device fill factor. Present work demonstrates a chemical linker with the capability of simultaneously grasping NiOx and perovskite crystals by forming a Ni−S−Pb bridge at buried interface to significantly boost the carriers transfer, based on a rationally selected molecule of 1,3‐dimethyl‐benzoimidazol‐2‐thione (NCS). The constructed buried interface not only reduces the pinholes and needle‐like residual PbI2 at the buried interface, but also deepens the work function and valence band maximum positions of NiOx, resulting in a smaller VBM offset between NiOx and perovskite film. Consequently, the modulated PSCs achieved a high fill factor up to 86.24 %, which is as far as we know the highest value in records of NiOx‐based inverted PSCs. The NCS custom‐tailored PSCs and minimodules (active area of 18 cm2) exhibited a champion efficiency of 25.05 % and 21.16 %, respectively. The unencapsulated devices remains over 90 % of their initial efficiency at maximum power point under continuous illumination for 1700 hours. [ABSTRACT FROM AUTHOR]

Providing safe, smooth, and efficient trajectories for autonomous vehicles has long been a question of great interest in the field of autopiloting. In dynamic and ever-changing urban environments, safe and efficient trajectory planning is fundamental to achieving autonomous driving. Nevertheless, the complexity of environments with multiple constraints poses challenges for trajectory planning. It is possible that behavior planners may not successfully obtain collision-free trajectories in complex urban environments. Herein, this paper introduces spatio–temporal joint optimization-based trajectory planning (SJOTP) with multi-constraints for complex urban environments. The behavior planner generates initial trajectory clusters based on the current state of the vehicle, and a topology-guided hybrid A* algorithm applied to an inflated map is utilized to address the risk of collisions between the initial trajectories and static obstacles. Taking into consideration obstacles, road surface adhesion coefficients, and vehicle dynamics constraints, multi-constraint multi-objective coordinated trajectory planning is conducted, using both differential-flatness vehicle models and point-mass vehicle models. Taking into consideration longitudinal and lateral coupling in trajectory optimization, a spatio–temporal joint optimization solver is used to obtain the optimal trajectory. The simulation verification was conducted on a multi-agent simulation platform. The results demonstrate that this methodology can obtain optimal trajectories safely and efficiently in complex urban environments. [ABSTRACT FROM AUTHOR]

This study investigates the role of IGFBP3-mediated m6A modification in regulating the miR-23a-3p/SMAD5 axis and its impact on fracture healing, aiming to provide insights into potential therapeutic targets. Utilizing fracture-related datasets, we identified m6A modification-related mRNA and predicted miR-23a-3p as a regulator of SMAD5. We established a mouse fracture healing model and conducted experiments, including Micro–CT, RT-qPCR, Alizarin Red staining, and Alkaline phosphatase (ALP) staining, to assess gene expression and osteogenic differentiation. IGFBP3 emerged as a crucial player in fracture healing, stabilizing miR-23a-3p through m6A modification, leading to SMAD5 downregulation. This, in turn, inhibited osteogenic differentiation and delayed fracture healing. Inhibition of IGFBP3 partially reversed through SMAD5 inhibition, restoring osteogenic differentiation and fracture healing in vivo. The IGFBP3/miR-23a-3p/SMAD5 axis plays a pivotal role in fracture healing, highlighting the relevance of m6A modification. IGFBP3's role in stabilizing miR-23a-3p expression through m6A modification offers a potential therapeutic target for enhancing fracture healing outcomes. [Display omitted] [ABSTRACT FROM AUTHOR]

Chondroitin sulfate (CS) and undenatured type II collagen (Col II) are the two major, biological macromolecules of cartilage-related tissues. In this study, a new extraction. process was developed to obtain CS and Col II simultaneously. By this process, CS. and undenatured Col II were extracted from sturgeon notochord with the yields of 5.34, ± 0.74% and 45.25 ± 5.25%, respectively. The SEC-RI-MALLS result showed that the, average molecular weight of notochord CS was 38.4 kDa. FTIR NMR, and SAX-HPLC, results indicated the notochord CS was mainly composed of CS-A. The new extraction, process had no effect on the triple helical structure of Col II. To analyze the interaction, between the two macromolecules, the effect of CS on Col II fibril formation was, examined using turbidity assay and SEM observation. CS accelerated the completion, of Col II self-assembly and inhibited the lateral aggregation of fibrils. The results of this, study suggested that the sturgeon notochord is a valuable source of CS and Col II. The. new extraction method not only improves the utilization rate of sturgeon notochord, but,also reduces the waste of aquatic resources. CS and Col II derived from sturgeon, notochord have the potential for use in biomedical materials [Display omitted] • Chondroitin sulfate (CS) and collagen (Col II) extraction method was developed. • Yields of CS and Col II from sturgeon notochord were suitable for industrialization. • Sturgeon notochord CS was mainly composed by CS-A with molecular weight of 38.4 kDa. • The method had no effect on secondary structure and fibril-forming ability of Col II. • CS bound on Col II fibrils and inhibited the lateral aggregation of fibrils. [ABSTRACT FROM AUTHOR]

• Umami peptides from enzymatic hydrolysate of Katsuwonus pelamis were investigated. • EAD-based micro-UPLC-QTOF-MS protocol was performed for peptides identification. • The taste property and umami-enhance effect of identified peptides were evaluated. • Taste mechanism was investigated by molecular docking and dynamics simulations. • It allowed for the comprehensive understanding of the molecular-level interactions. In this study, the enzymatic hydrolysates of skipjack tuna, Katsuwonus pelamis , were purified by ultrafiltration and further identified through micro-ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (micro-UPLC-QTOF-MS). The potential umami peptides were identified using both conventional collision-induced dissociation (CID) and novel electron-activated dissociation (EAD) fragmentation techniques. Nine novel umami peptides with iUmami-SCM > 588 were screened. Sensory evaluation and electronic tongue analysis were performed to confirm the taste characteristics of the umami peptides, indicating that these umami peptides all exhibited varying degrees of umami taste. Molecular docking and molecular dynamics simulation were utilized to investigate the interaction with T1R1/T1R3 taste receptors. The docking results revealed that Asp234, Ser23, Glu231, and Ile237 appeared most frequently in all docking sites and formed stable complexes through hydrogen bonding and electrostatic interactions. Furthermore, molecular dynamics simulation allowed for a more comprehensive analysis of their interactions within a dynamic environment, providing a deeper understanding of the umami perception mechanism involving umami peptides and receptors. [ABSTRACT FROM AUTHOR]

[Display omitted] • A green and sustainable extraction method of PC from S. platensis was established. • Pluronic F68, a temperature-sensitive polymer-based ATPS was evaluated and optimized. • The polymer can be recovered and reused, indicating a recyclable extraction process. • Interaction of PC and Pluronic F68 was studied by spectral and simulation approaches. • Hydrophobic segment of Pluronic F68 played a key role in the binding process with PC. In this study, a sustainable and environmentally friendly method was developed for the enrichment and purification of phycocyanin from Spirulina platensis. This was achieved by utilizing a temperature-sensitive polymer, Pluronic F68, in an aqueous two-phase solvent system. The phase behavior of the temperature-sensitive polymer-based biphasic system was evaluated. The extraction conditions were optimized by both single-factor experiments and response surface methodology. Under the optimal conditions, the upper polymer-rich phase was recycled for sustainable phycocyanin extraction, resulting in a grade of 3.23 during the third extraction cycle. Pluronic F68 could be efficiently recovered and reused during the extraction process. The interaction mechanism between Pluronic F68 and phycocyanin was systematically studied using FT-IR and fluorescence analysis. This was further complemented by static and dynamic calculation of molecular motion through molecular docking and molecular dynamics simulation, indicating that hydrophobic segment of Pluronic F68 played a key role in the binding process with phycocyanin. [ABSTRACT FROM AUTHOR]

[Display omitted] • Peptidomics method was used to identify and characterize antifreeze peptides. • Shrimp byproducts autolysate was employed for the discovery. • Possible antifreeze mechanisms were investigated by bioinformatics methods. • Bonding properties of antifreezes were studied using molecule docking. In the present study, a novel method based on peptidomics and bioinformatic was applied to identification and characterization of antifreeze peptides (AFPs) from shrimp byproducts autolysate (SBPA). According to the results of in silico prediction and high peptide structural inflexibility, DEYEESGPGIVH and EQICINFCNEK were picked as potential AFP-1 and AFP-2, respectively. The outcomes of DSC determination indicated that TH of synthesized AFP-1 and AFP-2 (10 mg/mL) were 1.37 °C and 1.57 °C, respectively. Besides, 0.1 %-3 % AFPs showed significant cryoprotection in shrimp muscle after 3 and 6 freeze–thaw cycles, evidenced by higher SSP content, Ca2+-ATPase activity, sulfhydryl content and lower surface hydrophobicity than control; while the higher concentration resulted in better protection against freeze induced denaturation. Both AFP-1&2 showed favorable hydrogen bonding affinity which facilitated ice binding and ice crystal growth inhibition. This work could provide new ideals for identification and characterization of AFPs. [ABSTRACT FROM AUTHOR]

In order to explore the synergistic action of β-cyclodextrin (β-CD) and sodium caseinate (SC) to stabilize the seal oil Pickering emulsion. Seal oil emulsions were prepared by β-CD (β-CD-E), SC (SC-E) and hybrid particles (β-CD@SC-E). Appearance and microscopic morphological analysis, particle size, ζ-potential and viscosity were used to characterize the emulsions. Droplets in emulsion stabilized by the hybrid particles were evenly, with smallest particle size (16.31 μm), largest absolute ζ-potential (27.85 mV) and higher apparent viscosity. Then, stability of the emulsions was evaluated by storage stability, centrifugation stability, pasteurization stability and salt stability. The results showed the appearance, microscopic morphology, particle size, creaming index (CI) of β-CD@SC-E were the least affected after storage, centrifugation, pasteurization and different salt concentration treatment. The interaction analysis indicated that blue shift happened in the maximum absorption wavelength, amide I of β-CD@SC. The α-helixes in β-CD@SC transformed to the β-fold and significant changes occurred in the crystal form and microstructure of β-CD@SC. All these results indicated that self-assembly occurred during the binding of β-CD and SC, which changed their original structures and enhanced their emulsification performance. Besides, hydrogen bonding and hydrophobic interaction played the vital roles during the self-assembly. • The hybrid particles of β-CD and SC were prepared through self-assembly. • The seal oil Pickering was stabilized by β-CD@SC hybrid particles. • β-CD and SC had synergistic action on the stability of the emulsion. • Hydrogen bonds and hydrophobic interactions played vital roles in β-CD@SC. [ABSTRACT FROM AUTHOR]

Purpose: Visualization of the implanted mesh after a pelvic floor repair surgery is important for evaluating mesh-related complications. We made an X-ray-detectable mesh and studied the histocompatibility and toxicity of it. Methods: A thin barium sulfate thread was weaved on a traditional polypropylene mesh to make it X-ray detectable. The cytotoxicity of the mesh was tested by the MTT assay on L929 cell line. The histocompatibility and toxicity of mesh were evaluated in rabbits. Meshes were first implanted intraperitoneally. On postoperative day 7, bloods were tested to estimate the acute toxicity of meshes. After 6 months, rabbits were sacrificed and local inflammatory reaction and tissue regeneration at implantation sites were estimated by the HE stain and Masson stain. In addition, CT scans were performed after surgeries to display the location and shape of implanted meshes. Results: Compared to the polypropylene mesh group, no significant difference was observed in the X-ray-detectable mesh group on both in vitro cytotoxicity and in vivo acute and chronic toxicity. The amounts of extra cellular matrix between two groups did not differ. Through CT scan and 3D remodeling, the barium sulfate thread clearly revealed the position and shape of the X-ray-detectable mesh, whereas the traditional mesh was invisible under CT scan. Conclusion: Adding a thin barium sulfate thread on the polypropylene mesh does not change its histocompatibility or toxicity in rabbit model. The barium sulfate thread can effectively show the location and shape of implanted mesh under CT scan. [ABSTRACT FROM AUTHOR]

The aerodynamic characteristics of high-speed trains operating in severe environment have gained an increasing interest in the past few years. As rain is a common weather phenomenon, understanding its effects on the aerodynamic characteristics of the high-speed train provides valuable information for the design of a train. In the present paper , the aerodynamic model of a high-speed train operating in a dry condition is first set up, and the simulation results are compared with the existing wind tunnel test data to validate accuracy of the mesh resolution and CFD technique. For the simulation of raindrops, the applicability of spherical raindrop assumption in numerical simulations is discussed in this paper. The results demonstrate that, when the equivalent spherical diameters are greater than 2 ​mm, the simulation errors of terminal velocity of raindrops exceed 3%. The non-spherical characteristics of raindrops need to be considered for large particle sizes, and the particle sphericity for various equivalent spherical diameters is investigated. After that, a two-way coupled Euler-Lagrange approach is developed to evaluate the aerodynamic characteristics of a high-speed train in heavy rain environment based on non-spherical raindrop assumption. The flow around the train, pressure coefficient distribution, skin friction coefficient distribution, and aerodynamic force coefficients of the high-speed train exposed to heavy rain environment are then investigated. It is found that the aerodynamic coefficients of the high-speed train increase approximately linearly with the rainfall intensity. The explicit equations for predicting the aerodynamic force coefficient which is correlated with train speed and rainfall intensity are proposed in this study. • The aerodynamic effect of heavy rain on the high-speed train is investigated using Euler-Lagrange approach. • The non-spherical characteristics of raindrops need to be considered for large raindrop sizes. • The aerodynamic coefficients of the high-speed train increase approximately linearly with the rainfall intensity. • The explicit equation of the aerodynamic force coefficient correlated with train speed and rainfall intensity is proposed. [ABSTRACT FROM AUTHOR]

• A REIMS method was developed for determination of whipping creams. • Lipid differences in non-dairy cream and milk fat cream were deciphered by OPLS-DA. • Rapid detection of minute non-dairy cream fraud in milk fat cream was achieved. • Machine learning-guided REIMS pattern recognition improved accuracy and reduced detection time. The illegal adulteration of non-dairy cream in milk fat cream during the manufacturing process of baked goods has significantly hindered the robust growth of the dairy industry. In this study, a method based on rapid evaporative ionization mass spectrometry (REIMS) lipidomics pattern recognition integrated with machine learning algorithms was established. A total of 26 ions with importance were picked using multivariate statistical analysis as salient contributing features to distinguish between milk fat cream and non-dairy cream. Furthermore, employing discriminant analysis, decision trees, support vector machines, and neural network classifiers, machine learning models were utilized to classify non-dairy cream, milk fat cream, and minute quantities of non-dairy cream adulterated in milk fat cream. These approaches were enhanced through hyperparameter optimization and feature engineering, yielding accuracy rates at 98.4–99.6%. This artificial intelligent method of machine learning-guided REIMS pattern recognition can accurately identify adulteration of whipped cream and might help combat food fraud. [ABSTRACT FROM AUTHOR]

A selective and sensitive procedure for determining seven hazardous microcystins in Margarya melanioides , using tandem mass spectrometry, is presented. The product ion m / z 135.2 was selected as representative for precursor ion scanning and used to increase overall sensitivity of this shotgun method. The method was validated, and recoveries ranged from 69.3 to 87.9%, with the relative standard deviation less than 10%. Limits of detection and quantitation were less than 3.1 and 9.3 ng·mL −1 , respectively. A total of 60 samples were tested using this method. 38 samples were found to be contaminated with microcystins, among which there are 31 samples whose estimated daily intake (EDI) was 0–7.8 times higher than the tolerable daily intake (TDI). This method would be useful for carrying out direct, ultra-rapid quantification of microcystins in foods, and is of significant interest for environmental and food safety applications. [ABSTRACT FROM AUTHOR]

Background: We assessed the efficacy of adding intraperitoneal (IP) chemotherapy to standard first-line intravenous (IV) chemotherapy in epithelial ovarian cancer (EOC) patients.Methods: Patients with stage IIIC-IV EOC who underwent optimal debulking surgery were randomly assigned to four cycles of weekly IP chemotherapy with cisplatin (50 mg/m2) and etoposide (100 mg/m2) followed by six cycles of IV chemotherapy every 3 weeks (IP/IV arm), or were administered IV chemotherapy alone (IV arm). The primary endpoint for this study was the 12-month non-progression rate (NPR).Results: Between 4/2009 and 9/2015, 218 patients were randomised, of whom 215 initiated treatment. In the IP/IV arm, 90.6% of patients completed 4 cycles of IP chemotherapy. The 12-month NPRs were 81.9% and 64.2% in the IP/IV and IV groups, respectively (HR 0.48 (95% CI 0.27-0.82)). The median progression-free survival (PFS) was increased in the IP/IV arm compared with that in the IV arm (22.4 vs. 16.8 months; HR 0.66 (0.48-0.91)) and in a subgroup with no gross cytoreduction (31.1 vs. 16.8 months; HR 0.46 (0.26-0.82)). Similar findings were detected with regard to time to first subsequent anticancer therapy (TFST) (25.9 vs. 18.0 months; P = 0.009) and time to second subsequent anticancer therapy (TSST) (40.8 vs. 30.1 months; P = 0.042). Grade 3/4 leukopenia, anaemia and gastrointestinal events were more common in the IP/IV arm, but the treatment burden was considered acceptable.Conclusions: IP chemotherapy prior to IV chemotherapy was associated with an increased 12-month NPR and a longer TSST than IV alone in patients with EOC, albeit with acceptable toxic effects. Long-term follow-up is warranted to identify the effects of IP therapy on overall survival. [ABSTRACT FROM AUTHOR]

Zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC) material was used as solid-phase extraction sorbent for purification of phospholipids from Hypophthalmichthys nobilis . The conditions were optimized to be pH 6, flow rate 2.0 mL·min −1 , loading breakthrough volume ⩽ 5 mL, and eluting solvent 5 mL. Afterwards, the extracts were analyzed by multi-dimensional mass spectrometry (MDMS) based shotgun lipidomics; 20 species of phosphatidylcholine (PC), 22 species of phosphatidylethanoamine (PE), 15 species of phosphatidylserine (PS), and 5 species of phosphatidylinositol (PI) were identified, with content 224.1, 124.1, 27.4, and 34.7 μg·g −1 , respectively. The MDMS method was validated in terms of linearity (0.9963–0.9988), LOD (3.7 ng·mL −1 ), LOQ (9.8 ng·mL −1 ), intra-day precision (<3.64%), inter-day precision (<5.31%), and recovery (78.8–85.6%). ZIC-HILIC and MDMS shotgun lipidomics are efficient for studying phospholipids in H. nobilis . [ABSTRACT FROM AUTHOR]

To compare the structural properties and biological activities of chondroitin sulfate (CS) in two different tissues of Chinese sturgeon (Acipenser sinensis) and Russian sturgeon (Acipenser gueldenstaedti) , we extracted their backbone cartilage CS (Cart-CS) and notochord CS (Noto-CS), and analyzed the CS structural properties using chromatographic and spectroscopic methods. The molecular weights of Chinese sturgeon Cart-CS and Noto-CS were 54.7 and 25.4 kDa, respectively, and the molecular weights of Russian sturgeon were 50.0 and 38.4 kDa, respectively. The disaccharide composition results showed that Cart-CS was mainly composed of CS-C, while Noto-CS was almost composed of pure CS-A. The antioxidant activity of sturgeon CS and its effect on collagen fibril formation were discussed. Sturgeon CS exhibited higher antioxidant activity than shark and bovine CSs. Sturgeon CS inhibited the self-assemble of type I collagen into fibrils. The inhibition effect of Cart-CS was higher than that of Noto-CS. The high value-added utilization of Cart-CS and Noto-CS will increase the value of sturgeon by-products. Furthermore, the disaccharide composition of CS in sturgeon depends on tissues of origin, but not on species. It means that the CS of Chinese sturgeon can be substituted by the CS of other commercial sturgeon. That will contribute to the protection of endangered species of Chinese sturgeon from illegal fishing and increase the value of commercial sturgeon by-products. [Display omitted] • Two kinds of CS were obtained from Acipenser sinensis and Acipenser gueldenstaedti. • The average molecular weight of cartilage CS was higher than that of notochord CS. • Cartilage CS was mainly composed by CS-C; Notochord CS was almost composed by CS-A. • The inhibition of CS-C on collagen fibril-forming was higher than that of CS-A. • The CS from Acipenser sinensis can be substituted by that from commercial sturgeon. [ABSTRACT FROM AUTHOR]

A micro-scale matrix solid-phase dispersion (MSPD) technique, using hydrophilic–lipophilic balance (HLB) material as sorbent and a pipette tip (PT) as the cartridge, was developed for the extraction and purification of sulfonamides in fish tissue. Eluates from PT-MSPD were analyzed using fast liquid chromatography and tandem mass spectrometry (LC–MS/MS). The method was fully validated; good linearity was obtained with correlation coefficients greater than 0.99. Precision and accuracy (RSD%) were in the range 1.4–10.3% while mean recoveries were 70.6–95.5%. With this technique, 15 aquatic samples ( Collichthys niveatus ) were analyzed for sulfonamides. The whole procedure took only 13 min (5 min for PT-MSPD and 8 min for LC), materials for each sample included 5.1 mL solvents (0.3 mL for PT-MSPD and 4.8 mL for LC), and 20 mg HLB sorbent. Generally speaking, this method is indeed practical and particularly suitable for widespread drug residue analysis. [ABSTRACT FROM AUTHOR]

The surimi processing industry generates a large number of underutilized by-products. The aim of this study was to evaluate the protein oxidation, protein degradation, and gel property of surimi during frozen storage to investigate the mechanism of cryoprotective effects of surimi by-product protein hydrolysates on surimi. The results showed that compared with Control and Cryoprotectant surimi, surimi by-product protein hydrolysates groups had lower thiobarbituric acid content, carbonyl content and surface hydrophobicity, higher Ca2+-ATPase activity, total sulfhydryl groups and salt-soluble proteins (P < 0.05), which significantly delayed the freezing denaturation and oxidation rate of surimi myofibrillar protein during frozen storage. Aggregation of surimi proteins occurred during frozen storage, and surimi by-product protein hydrolysates could inhibit the degradation of myofibrillar protein and effectively induce protein cross-linking, which led to the formation of a denser gel network in surimi. In summary, surimi by-product protein hydrolysates was expected to be a natural and green cryoprotectant and gel texture enhancer in surimi. [Display omitted] • Surimi by-products protein hydrolysates inhibited the protein oxidation of surimi. • Surimi by-products protein hydrolysates inhibited the protein denaturation of surimi. • Surimi by-products protein hydrolysates improved the gel property and microstructure of surimi. • Surimi by-products protein hydrolysates could be used as a natural cryoprotectant and gel texture enhancer of surimi. [ABSTRACT FROM AUTHOR]

Crosswind associated with rainfall is a common weather condition, which threatens the operational safety of trains. This paper focuses on an investigation of the effect of crosswind associated with rainfall on train aerodynamics. The methodologies for reproducing the turbulent wind speed and instantaneous rainfall intensity are first described, and the calculation method of unsteady aerodynamic forces of trains operating in a wind-rain environment is set up using the quasi-steady method and weighting function method. Then the CFD technology is used to obtain the aerodynamic load coefficients of a train exposed to a wind-rain environment, and the computational model is verified with the experimental results. Finally, the unsteady aerodynamic load time histories, as well as the corresponding statistical characteristics of the train are investigated. The results demonstrate that the aerodynamic load coefficient is a function of yaw angle, rainfall intensity and wind velocity relative to the train under wind-rain conditions. The aerodynamic loads approximately obey normal distribution both for the quasi-steady method and weighting function method. The mean values of aerodynamic loads obtained by the two methods are almost the same, whereas the standard deviations computed by the weighting function method are smaller than those computed by the quasi-steady method. The dimensionless standard deviations of aerodynamic loads increase with the rainfall intensity, and are approximately linearly related to the rainfall intensity. The wind velocity relative to the train also has an impact on the dimensionless standard deviations of aerodynamic loads, and the impact becomes more obvious when the rainfall intensity increases. The dimensionless standard deviations of the aerodynamic loads can be expressed as quadratic polynomial functions of yaw angle, rainfall intensity and wind velocity relative to the train. • The formula for computing the unsteady aerodynamic forces of a train under the combined wind and rain action is proposed. • The statistical properties of unsteady aerodynamic loads of a train operating in wind-rain environment are investigated. • The unsteady aerodynamic loads of a train in wind-rain condition fluctuate more violent than those in crosswind condition. • The dimensional standard deviations of aerodynamic loads increase with rainfall intensity. [ABSTRACT FROM AUTHOR]

[Display omitted] • Ultrasonic-assisted extraction of fucoxanthin from S. fusiforme was investigated. • Ethyl lactate, limonene, and vegetable oils were compared for effective extraction. • The extraction conditions were optimized by single-factor and response surface tests. • The second-order kinetic model was fit to describe the dynamic extraction process. The development of green and sustainable extraction technologies for various naturally active biomaterials is gaining increasing attention due to their environmentally friendly advantages. In this work, the ultrasonic-assisted extraction of fucoxanthin from edible brown algae Sargassum fusiforme using different green solvents was presented. Ethyl lactate, limonene, soybean oil, and sunflower oil were used in place of traditional organic solvents. Ethyl lactate showed similar performance to organic solvents, whereas limonene and vegetable oil exhibited higher selectivity for fucoxanthin. Moreover, the effects of various extraction factors, including liquid/solid ratio, extraction time, extraction temperature, as well as amplitude were studied. The optimal conditions were optimized as follows: liquid/solid ratio, 40 mL/g; extraction time, 27 min; extraction temperature, 75 ℃; amplitude, 53%; and solvent, ethyl lactate. Optimal model of second-order kinetic parameters (rate constant, equilibrium concentration, and initial extraction rate) was successfully developed for describing the dynamic ultrasonic extraction process under different operating conditions. [ABSTRACT FROM AUTHOR]

Objective: To analyze the clinical efficacy of ultrasonic bone scalpel (UBS)-assisted unilateral biportal endoscopic lamina osteotomy replantation (ULOR) for treating lumbar infectious spondylitis (LIS)., Methods: We conducted a retrospective analysis of patients with LIS who had therapy with UBS-assisted ULOR at our hospital between January 2020 and May 2023. A total of 17 instances matched the inclusion criteria, consisting of 7 females and 10 males. The UBS was utilized during surgery to extract the lamina completely, followed by large bone grafting to fuse the cleaned intervertebral space. The study recorded and analyzed various parameters in the included patients before and after surgery., Results: All 17 patients underwent a successful operation with an average duration of 136.82 ± 21.35 minutes, average blood loss of 77.43 ± 10.19 ml, and an average follow-up period of 18.55 ± 3.47 months. Following the surgical intervention, the patients experienced a substantial improvement in their clinical symptoms, accompanied by a significant drop in white blood cell, erythrocyte sedimentation rate, and C-reactive protein levels (all P < 0.001). The postoperative visual analog scale scores and Oswestry Disability Index showed considerable improvement (both P < 0.001). The postoperative Cobb angle and intervertebral space height were significantly corrected (P < 0.001). At the last follow-up, the success rate of lumbar fusion was 100%, and there were no instances of infection recurring., Conclusions: The use of ULOR with assistance from UBS for the treatment of LIS has proven to be beneficial. It provides significant relief from symptoms and corrects lumbar deformity. This surgical procedure is both effective and safe., (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)

Distributed drive electric vehicles improve steering response and enhance overall vehicle stability by independently controlling each motor. This paper introduces a control framework based on Adaptive Model Predictive Control (AMPC) for coordinating handling stability, consisting of three layers: the dynamic supervision layer, online optimization layer, and low-level control layer. The dynamic supervision layer considers the yaw rate and maneuverability limits when establishing the β-β˙ phase plane stability boundary and designs variable weight factors based on this stability boundary. The online optimization layer constructs the target weight-adaptive AMPC strategy, which can adjust the control weights for maneuverability and lateral stability in real time based on the variable weight factors provided by the dynamic supervision layer. The low-level control layer precisely allocates the driver's requested driving force and additional yaw moment by using torque distribution error and tire utilization as the cost function. Finally, experiments are conducted on a Simulink-CarSim co-simulation platform to assess the performance of AMPC. Simulation results show that, compared to the traditional MPC strategy, this control strategy not only enhances maneuverability under normal conditions but also improves lateral stability control under extreme conditions.

The present study was aimed to obtain a close insight into the distribution and diversity of antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs) among the aquatic products collected in Zhejiang, China. A total of 136 presumptive ARB picked up from six aquatic samples were classified into 22 genera and 49 species based on the 16S rDNA sequencing. Aeromonas spp., Shewanella spp., Acinetobacter spp., Myroides spp., Pseudomonas spp., and Citrobacter spp. accounted for 80% of the ARB. Among them, 109 isolates (80.15%) exhibited resistance to at least one antibiotic. Most isolates showed resistance to not only the originally selected drug but also to one to three other tested drugs. The diversity of ARB distributed in different aquatic products was significant. Furthermore, the resistance data obtained from genotypic tests were not entirely consistent with the results of the phenotypic evaluation. The genes qnr S, tet A, flo R, and cml A were frequently detected in their corresponding phenotypic resistant isolates. In contrast, the genes sul 2, aac (6')-Ib, and bla PSE were less frequently found in the corresponding phenotypically resistant strains. The high diversity and detection rate of ARB and ARGs in aquaculture might be a significant threat to the food chains closely related to human health., (© 2023 JIAJIA WU et al., published by Sciendo.)

Salted white herring (Ilisha elongata) is a popular fish product in the coastal region of China. The complex endogenous enzymes and microbial action determine the quality of a traditionally salted herring. In order to investigate the role of microorganisms in the quality formation of salted herring, three groups for different salting processes were established: traditional salted (TS), non-starter salted (NS), and starter culture salted (SS). The predominant microorganism in each processing group was Staphylococcus spp., as inferred by next-generation sequencing data. Different physicochemical parameters were obtained in each of the three processing groups (TCA-soluble peptide (trichloroacetic acid-soluble peptide), TVB-N (Total volatile basic nitrogen), and TBA values (thiobarbituric acid-reactive substance)). The TS group had the maximum level of total biogenic amines, while the SS group had the lowest. A strong positive correlation was found between Staphylococcus and 14 aromatic compounds, of which 5 were odor-active compounds that created fishy, grassy, fatty, and fruity flavors. Shewanella may produce trimethylamine, which is responsible for the salted herrings’ fishy, salty, and deteriorating flavor. The findings demonstrated that autochthonous strains of Staphylococcus saprophyticus M90−61 were useful in improving product quality because they adapted quickly to the high osmotic environment.

It is still difficult to conduct numerical calculation of the aerodynamic noise of full-scale, long-marshalling, high-speed trains. Based on the Lighthill acoustic analogy theory, the aerodynamic sound source of the high-speed train is equivalent to countless micro-vibrating sound sources. An acoustic radiation model of the dipole sound source of high-speed trains is established, and a method to predict the aerodynamic noise in the far field of long-marshalling high-speed trains is proposed. By this method, combined with numerical simulation technology, the flow field, noise source, and far-field noise characteristics of high-speed trains with different marshalling numbers are studied. The improved delayed detached eddy simulation method is used for flow field calculation, to obtain aerodynamic noise source information regarding the surface of high-speed trains. The numerical calculation method is verified by wind tunnel testing. The results show that the flow field and noise source characteristics of high-speed trains with different marshalling numbers are similar. The greater the length of the train body, the longer the trailing distance of the train wake, and the stronger of a surface noise source the tail car becomes. The spatial distribution characteristics of aerodynamic noise in the far field of high-speed trains do not change significantly with the length of the train body, but the magnitude of the sound pressure level will increase with the increase in length of the train body. The middle car body parts of high-speed trains with different marshalling numbers have similar noise distributions and sound pressure levels. Based on the noise calculation results of the 3-marshalling high-speed train, the far-field noise of the 5-marshalling and 8-marshalling train models is predicted and found to be in good agreement with the far-field noise of the actual train model. The differences in average sound pressure level are 1.01 dBA and 1.74 dBA, respectively., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

The effects of astaxanthin extract (AE) from shrimp by-products on the quality and sensory properties of ready-to-cook shrimp surimi products (RC-SSP) during frozen storage at −18 °C were investigated. Changes in 2-thiobarbituric acid reactive substances (TBARS) value, sulfhydryl groups, carbonyls, salt-soluble protein content, textural properties, color, and sensory quality over specific storage days were evaluated. The AE from shrimp by-products contained 4.49 μg/g tocopherol and 23.23 μg/g astaxanthin. The shrimp surimi products supplemented with 30 g/kg AE had higher redness values and greater overall acceptability and texture properties after cooking (p < 0.05). AE showed higher oxidative stability in RC-SSP than the control, as evidenced by lower TBARS and carbonyl content, and higher sulfhydryl and salt-soluble protein content. AE from shrimp by-products had positive effects on the antioxidant activity and color difference of RC-SSP, and could be used as a potential multifunctional additive for the development of shrimp surimi products.

Introduction and Hypothesis: Traditionally, surgical treatment for uterine prolapse has included hysterectomy. However, more patients now prefer a uterine-preserving operation because of concerns about fertility or sexual dysfunction. In this video, we describe a novel approach to correcting uterine prolapse in an attempt to demonstrate an alternative option for patients., Methods: A 42-year-old woman with symptomatic stage I-IV uterine prolapse (POP-Q: Aa +2, Ba +2, C + 3, gh 6.5, pb 3, TVL 8.5, Ap 0, Bp 0, D 0) underwent inguinal ligament suspension. The principle steps and techniques to complete the operation are outlined in the video., Results: Prolapse repair was successfully completed without any intraoperative complications. The uterus was restored to its anatomic position. During the 12-month follow-up, neither recurrence nor postoperative complications, such as mesh exposure, de novo incontinence or bowel obstruction, etc., occurred., Conclusions: Laparoscopic inguinal ligament suspension is a safe and feasible alternative for correcting the uterine prolapse. This surgery could be an attractive choice for patients who prefer a uterine-sparing surgery.

Objective: To introduce an alternative surgical technique of laparoscopic inguinal ligament suspension (LILS) with uterine preservation and evaluate its efficacy and safety for patients with severe pelvic organ prolapse (POP)., Methods: Between June 2014 and December 2015, 35 patients with symptomatic stage III or IV were treated by LILS with uterine preservation. The perioperative parameters including surgical time, blood loss, hospital stay and complications were recorded. The anatomical cure rate was evaluated according to the Pelvic Organ Prolapse Questionnaire (POP-Q) assessment. The anatomical points were analyzed by dynamic Magnetic Resonance Imaging (MRI). Validated questionnaire of the Pelvic Floor Distress Inventory (PFDI-20), the Pelvic Floor Impact Questionnaire (PFIQ-7) and the Pelvic organ prolapse urinary Incontinence Sexual Questionnaire (PISQ-12) were recorded to evaluate the symptom severity, quality of life and sexual activity., Results: The mean surgical time was 163.8 ± 42.3 min (range: 120-280 min), the mean estimated blood loss was 48.6 ± 60.5 ml (range: 10-200 ml), and the mean hospital stay was 5 days (range: 3-7 days). No intra-operative complications were encountered. The anatomical success rate at postoperative 6-month and 12-month was 97.1% and 94.3%, respectively. The postoperative anatomical points on straining showed a significant improvement on dynamic MRI as compared to baselines. The symptom severity, quality of life and sexual activity also presented significant improvement both 6-month and 12-month after surgery. After a minimal 12 months follow-up, no postoperative complications occurred and the recurrence prolapse were low., Conclusion: LILS with uterine preservation is a feasible, effective and safe surgical alternative in the treatment of POP for patients who desire to reserve uterus. Longer follow-up data from larger studies are required to confirm the benefits of LILS with uterine preservation as a minimally invasive surgical approach., (Copyright © 2018 IJS Publishing Group Ltd. Published by Elsevier Ltd. All rights reserved.)

Background: Pelvic organ prolapse (POP) is a common disease in women. The aim of this research was to evaluate the safety, efficacy and complication of transvaginal modified sacrospinous ligament fixation with mesh using for the treatment of vaginal vault prolapse., Materials and Methods: This was a prospective study including information from 60 symptomatic women with anterior-apical pelvic floor prolapse. The patients underwent transvaginal modified sacrospinous ligament fixation combined with anterior vaginal wall mesh between May 2014 and Sep 2015. The perioperative data including clinical characteristic, operation time, blood loss, and surgical complications were collected at 1 year and 2 years. During a 2-year follow-up, the primary outcome evaluation included Pelvic organ prolapse Quantification system (POP-Q), Incontinence Quality of Life scale (I-QoL), the Prolapse/Urinary Incontinence Sexual Questionnaire (PISQ-12) and the Pelvic Floor Distress Inventory, short form 20 (PFDI-20)., Results: The mean follow-up time was 2 years (range 24-37 months). The patients' mean age was 66.75 ± 6.44. Of 60 patients who were enrolled in this research, 26 patients had severe stress urinary incontinence (SUI). The mean operation time was 99.14 ± 19.60 min and the mean estimated blood loss was 73.83 ± 41.05 ml. The rate of anatomical success was 98.3% and one patient had a recurrence. The POP-Q point measurements were evaluated preoperatively and postoperatively (P < 0.001). Moreover, the quality of life and sexual activity were all improved postoperatively via I-QoL, PISQ-12 and PFDI-20 scores (P < 0.001). There was no injury to the rectum, bladder, major pelvic vessels and pudendal nerves. However, 18 patients had postoperative complications., Conclusions: This study showed that transvaginal modified sacrospinous ligament fixation with mesh might be performed easily and might be a safe surgery for elderly patients whose requirements for sexual life were relatively low. Further researches were required to investigate its long-term efficacy., (Copyright © 2018 IJS Publishing Group Ltd. Published by Elsevier Ltd. All rights reserved.)

Introduction: The aim of the study was to evaluate the efficacy and safety of laparoscopic inguinal ligament suspension (LILS) as a new surgical technique for the treatment of vaginal vault prolapse., Methods: From Feb 2014 to Mar 2016, 21 symptomatic women with grades III-IV vaginal vault prolapse were enrolled. All patients underwent LILS procedure in which a bifurcated mesh was used to suspend the vaginal vault to inguinal ligament. The perioperative parameters including surgical time, blood loss, and hospitalization time were recorded. At a minimal 12-month follow-up, the primary outcome measures, such as the anatomical cure rate and patients' satisfaction were respectively evaluated according to Pelvic organ prolapse questionnaire (POP-Q) and Patient Global Impression of Improvement (PGI-I) scale. The secondary outcomes including the impact on symptom severity, quality of life and sexual activity were also recorded., Results: The mean surgical time was 130.71 ± 16.07 min, the mean estimated blood loss was 53.57 ± 48.43 ml, and the mean hospital stay was 6 days (range: 5-8 days). After a minimal 12-month follow-up (range: 12-36 months), the rate of anatomical success and the subjective satisfaction were 100% and 90.5%. The symptom severity, quality of life and sexual activity also presented significant improvement. In addition, no serious peri- and postoperative complications occurred., Conclusions: LILS was a potential method for the treatment of vaginal vault prolapse. The technique could be performed easily and might be an alternative to other POP surgeries. However, further studies were required to focus on its long-term efficacy., (Copyright © 2017 IJS Publishing Group Ltd. Published by Elsevier Ltd. All rights reserved.)

Dried seahorse is a precious raw food material for cooking soups. In this study, a lipidomics strategy using the techniques of solid-phase extraction (SPE) and hydrophilic interaction chromatography-tandem mass spectrometry (HILIC-QTOF/MS) was developed for extraction, visualization, and quantification of phospholipids in dried seahorses. The parameters of SPE were optimized, and 1 mL of sample and chloroform/methanol (1:2, v/v) were found to be the best loading volume and eluting solvent, respectively. Afterwards, each phospholipid class was successfully separated on a HILIC column and analyzed by mass spectrometry. A total of 50 phospholipid molecular species were identified and determined, including 15 phosphatidylcholines (PCs), 14 phosphatidylethanolamines (PEs), 12 phosphatidylinositols (PIs) and 9 phosphatidylserines (PSs). In comparison to previously methods, this strategy was robust and efficient in extraction, characterization, and determination of phospholipids. The dried seahorse was found to contain large amounts of polyunsaturated fatty acyl phospholipids which are beneficial to human health., (Copyright © 2016 Elsevier Ltd. All rights reserved.)

Objective: To assess the outcomes of patients who underwent local excision of adenomyoma and to evaluate the effectiveness of the procedure., Study Design: Prospective study of 86 women undergoing local excision of symptomatic uterine adenomyoma at laparotomy. Dysmenorrhea was assessed before and after operation by questionnaire, and the average duration of follow-up was 24 months., Result(s): At 6-month follow-up, all 86 patients' dysmenorrhea grades were reduced by more than 80%. With a mean follow-up of 24 (range 6-60) months, a total of 2 (2.3%) patients finally underwent hysterectomy, 1 (1.2%) received levonorgestrel-releasing intrauterine device therapy, and three patients (11.3%) were subsequently given treatment with oral pain medication alone. The mean time interval between local excision and subsequent treatment was 34 months. Two (2.3%) patients became pregnant after the local excision: one (1.2%) at 2 months after the procedure and one (1.2%) at 3 years after the local excision procedure., Conclusion(s): Local excision of uterine adenomyoma remains a safe and effective minimally invasive treatment for women with symptomatic uterine adenomyoma. Local excision can effectively alleviate the dysmenorrhea of adenomyoma., (Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.)

An ultrafast analytical protocol based on online solid-phase extraction (SPE)/high-performance liquid chromatography-tandem mass spectrometry for the determination of resveratrol in red wines has been developed. In the present work, multiwalled carbon nanotubes (MWCNTs) were used as SPE sorbents for the analytes' online extraction and cleanup. The target analytes were separated on a fused-core C18-silica column (Halo, 50 mm × 2.1 mm i.d., 2.7 μm) and quantified by triple-quadrupole linear ion trap mass spectrometry in negative ion multiple-reaction monitoring (MRM) mode. The proposed analytical procedures were carefully optimized and validated. The calibration function is linear from 0.37 to 370 ng mL(-1) and from 0.13 to 130 ng mL(-1) for trans- and cis-resveratrol, respectively. The limits of quantification (LOQs) of trans- and cis-resveratrol obtained were 0.05 and 0.06 ng mL(-1), which means that the proposed method is suitable for trace analysis of resveratrol at low-level concentration. At the three fortified levels (low, medium, and high), recoveries of resveratrol ranging from 76.9 to 108.3% were obtained. Eight red wine samples from different regions of China were analyzed. The results indicated that the present online SPE-LC-MS/MS system significantly increased sample throughput and decreased solvent consumption, exhibiting great potential to be applied for analyzing resveratrol in red wines.

A simple and effective multi-residue analysis method is presented for the extraction and determination of eleven quinolones (pipemidic acid, enoxacin, norfloxacin, ciprofloxacin, lomefloxacin, enrofloxacin, gatifloxacin, difloxacin, oxolinic acid, nalidixic acid and flumequine) in fish tissues. In this study, multi-residue separations on four columns packed with 5 microm or sub-2 microm particles were simultaneously developed for the purpose of comparison. Various gradients were optimized and best resolutions were achieved on each column. A short and sub-2 microm particle-sized HPLC column was chosen for its advantages in analysis time and column performance. Additionally, considering the matrix effect of the complex crude fish tissue, an effective extraction protocol was also established for sample pre-treatment procedure. Good recoveries (71-98%) were obtained from samples fortified with a mix of eleven quinolones at three levels, with satisfactory relative standard deviations and limits of detection. As a result, the sub-2 microm HPLC column and proposed analytical procedures have been evaluated and applied to the analysis of different fish tissues. Detectable residues were observed in 8 of 30 samples, at concentrations ranging from 4.74 to 23.27 microg/kg.