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Although cowpea mild mottle virus (CPMMV) has been reported in Brazil since 1983, it has only become a significant concern for researchers and farmers in recent years. The objective of this work was to investigate the genetic basis of CPMMV tolerance, mapping and reporting the first loci associated with this trait in common bean (cv. BRS Sublime). Phenotypic assays were carried out on 180 individual plants (F2 generation) and 180 F2:3 progenies comprising 12 plants per family and their parents (BRS Sublime, tolerant parent × CNFCT 16207, susceptible parent). CPMMV was mechanically inoculated and symptoms were evaluated at 35 days after inoculation, using a 1–5 scoring scale. A linkage map was constructed using 1695 single‐nucleotide polymorphism (SNP) and SilicoDArT markers that segregated in the F2 and F2:3 generations as expected. Markers were distributed across 11 common bean chromosomes, resulting in a total length of 2864 cM, with an average distance between markers of 1.8 cM. Phenotypic observations revealed that tolerance in cv. BRS Sublime is controlled by a single dominant gene. The main effect quantitative trait locus (QTL; CPMMV.Pv08) associated with CPMMV tolerance was identified in the terminal region on chromosome 8 (Pv08). This QTL explained approximately 77% of phenotypic variation, indicating that the inheritance of tolerance to CPMMV is monogenic, controlled by a major locus. As far as we know, this study represents the first investigation into the inheritance and genetic mapping of CPMMV tolerance in common bean, with potential for the development of elite lines with multiple virus resistance/tolerance. [ABSTRACT FROM AUTHOR]

The purpose of this review is to provide a comprehensive framework for researchers, academics, and food developers regarding the techno-functional properties of the most consumed legume proteins globally, including peas, cowpeas, common beans, chickpeas, fava beans, lentils, and lupins. The review delves into the structural aspects of these proteins in detail and the techniques employed in their extraction and modification. Detailed information is provided on various techno-functional properties, such as solubility, water and oil retention capacity, emulsifying properties, foaming properties, and gelation properties of legume proteins. Practical applications in various food products are highlighted, including bakery items, pasta, snacks, dairy alternatives, sauces, meat analogues, and hybrid products. The review also explores emerging uses, such as the role of these proteins as encapsulating materials and even in 3D food printing. In addition, suggestions for future applications are presented, emphasizing their relevance in the development of innovative products and promoting the comprehensive utilization of legume proteins in the food industry. The importance of these innovations is emphasized in attracting consumers and facilitating a successful transition toward more sustainable plant-based diets. [ABSTRACT FROM AUTHOR]

Soil salinization significantly impacts agricultural lands and crop productivity in the study area. Moreover, freshwater scarcity poses a significant obstacle to soil reclamation and agricultural production. Therefore, eco-friendly strategies must be adopted for agro-ecosystem sustainability under these conditions. A study conducted in 2022 and 2023 examined the interaction effects of various soil mulching materials (unmulched, white plastic, rice straw, and sawdust) and chitosan foliar spray application (control, 250 mg L−1 of normal chitosan, 125 mg L−1 of nano chitosan, and 62.5 mg L−1 of nano chitosan) on the biochemical soil characteristics and productivity of common beans in clay-saline soil. Higher organic matter, available nutrient content, and total bacteria count in soils were found under organic mulching treatments (rice straw and sawdust). In contrast, the white plastic mulching treatment resulted in the lowest values of soil electrical conductivity (EC) and the highest soil water content. Conversely, chitosan foliar spray treatments had the least impact on the chemical properties of the soil. Plants sprayed with 62.5 mg L−1 of nano chitosan exhibited higher chlorophyll content, plant height, fresh weight of shoots and roots, seed yield, and nutrient content compared to other chitosan foliar spray applications. All treatments studied led to a significant reduction in fungal communities and Na% in plants. The combined effect of organic mulch materials and foliar spray application of 62.5 mg L−1 nano chitosan appeared to enhance biochemical saline soil properties and common bean productivity. [ABSTRACT FROM AUTHOR]

The concentration of anthocyanins in common beans indicates their nutritional value. Understanding this concentration makes it possible to identify the functional compounds present. Previous studies have presented color characterization as two-dimensional histograms, based on the probability mass function. In this work, we proposed a new type of color characterization represented by three two-dimensional histograms that consider chromaticity and luminosity channels in order to verify the robustness of the information. Using a neuroevolutionary approach, we also found a convolutional neural network (CNN) for the regression task. The results demonstrate that using three two-dimensional histograms increases the accuracy compared to the color characterization represented by one two-dimensional histogram. As a result, the precision was 93.00 ± 5.26 for the HSI color space and 94.30 ± 8.61 for CIE L*a*b*. Our procedure is suitable for estimating anthocyanins in homogeneous and heterogeneous colored bean landraces. [ABSTRACT FROM AUTHOR]

Drought stress is widely recognized as a highly detrimental abiotic stress factor that significantly impacts crop growth, development, and agricultural productivity. In response to external stimuli, plants activate various mechanisms to enhance their resistance or tolerance to abiotic stress. The common bean, a most important legume according to the FAO, serves as a staple food for millions of people worldwide, due to its rich protein, carbohydrate, and fiber content, concurrently, and water scarcity is the main factor limiting common bean production. The process of domestication and on-farm conservation has facilitated the development of genotypes with varying degrees of drought stress resistance. Consequently, using landraces as biological material in research can lead to the identification of variants with superior resistance qualities to abiotic stress factors, which can be effectively integrated into breeding programs. The central scope of this research was to find out if different geographic origins of common bean genotypes can determine distinct responses at various levels. Hence, several analyses were carried out to investigate responses to water scarcity in three common bean genotypes, M-2087 (from the Mesoamerican gene pool), A-1988 (from the Andean gene pool) and Lechinta, known for its high drought stress resistance. Plants were subjected to different water regimes, followed by optical assessment of the anatomical structure of the hypocotyl and epicotyl in each group; furthermore, the morphological, physiological, and biochemical parameters and molecular data (quantification of the relative expression of the thirteen genes) were assessed. The three experimental variants displayed distinct responses when subjected to 12 days of water stress. In general, the Lechinta genotype demonstrated the highest adaptability and drought resistance. The M-2087 landrace, originating from the Mesoamerican geographic basin, showed a lower resistance to water stress, compared to the A-1988 landrace, from the Andean basin. The achieved results can be used to scale up future research about the drought resistance of plants, analyzing more common bean landraces with distinct geographic origins (Mesoamerican/Andean), which can then be used in breeding programs. [ABSTRACT FROM AUTHOR]

The increasing need for new treatments for obesity and diabetes has led to the development of new drugs and food supplements that could reduce carbohydrate absorption. Many starch blockers, based on common bean proteinaceous inhibitors against α-amylase (α-AI), are already present on the market. The extraction and purification of α-amylase inhibitor from a promising common bean cultivar from Sardinia (Nieddone) is described, highlighting the unique value of the Nieddone cultivar, particularly for its inhibitory activity on digestive enzymes and its complete lack of a hemagglutination effect on human red blood cells. The purification of α-AI involved two chromatographic steps (IEC and SEC) and was essential for revealing certain properties of the inhibitor. The purified inhibitor has a tetrameric structure (α2β2) and a molecular weight of approximately 42 kDa, as determined by SEC and SDS-PAGE, confirming it as a lectin-like inhibitor. The identification of the α-AI sequence was obtained by bottom-up high-resolution mass spectrometry, which allowed us to identify a unique peptide from the α chain and six unique peptides from the β chains. α-AI exhibited an optimum temperature of around 40 °C and two pH optima at 5 and 6.5, respectively. Its remarkable stability at high temperatures was measured (approximately 25% of activity retained even after 5 h at 100 °C), whereas the raw extract lost its activity entirely after just 10 min at 90 °C. Thus, the purification process significantly enhances the thermal stability of α-AI. The demonstrated effectiveness of the purified α-AI against the α-amylase enzyme in pigs, humans and insects underscores the protein's potential for treating obesity and diabetes, as well as for managing insect pests. [ABSTRACT FROM AUTHOR]

The common bean (Phaseolus vulgaris L.) is a globally cultivated leguminous crop. Fusarium wilt (FW), caused by Fusarium oxysporum f. sp. phaseoli (Fop), is a significant disease leading to substantial yield loss in common beans. Disease-resistant cultivars are recommended to counteract this. The objective of this investigation was to identify single nucleotide polymorphism (SNP) markers associated with FW resistance and to pinpoint potential resistant common bean accessions within a core collection, utilizing a panel of 157 accessions through the Genome-wide association study (GWAS) approach with TASSEL 5 and GAPIT 3. Phenotypes for Fop race 1 and race 4 were matched with genotypic data from 4740 SNPs of BARCBean6K_3 Infinium Bea Chips. After ranking the 157-accession panel and revealing 21 Fusarium wilt-resistant accessions, the GWAS pinpointed 16 SNPs on chromosomes Pv04, Pv05, Pv07, Pv8, and Pv09 linked to Fop race 1 resistance, 23 SNPs on chromosomes Pv03, Pv04, Pv05, Pv07, Pv09, Pv10, and Pv11 associated with Fop race 4 resistance, and 7 SNPs on chromosomes Pv04 and Pv09 correlated with both Fop race 1 and race 4 resistances. Furthermore, within a 30 kb flanking region of these associated SNPs, a total of 17 candidate genes were identified. Some of these genes were annotated as classical disease resistance protein/enzymes, including NB-ARC domain proteins, Leucine-rich repeat protein kinase family proteins, zinc finger family proteins, P-loopcontaining nucleoside triphosphate hydrolase superfamily, etc. Genomic prediction (GP) accuracy for Fop race resistances ranged from 0.26 to 0.55. This study advanced common bean genetic enhancement through marker-assisted selection (MAS) and genomic selection (GS) strategies, paving the way for improved Fop resistance.

ABSTRACT Low soil mineral concentrations are a major limitation to the nutritional quality of grain crops produced in Africa. As a result, 232 million people are suffering from microelement deficiency and 239 million from protein‐calorie malnutrition in Africa. This study evaluated the nutritional quality of common bean grain harvested from 63 genotypes planted at Malkerns in Eswatini. The results showed significantly marked differences in the concentrations of 10 dietarily important nutrient elements. Of the macronutrients, Na levels showed the highest variation (12.00–91.00 mg/g) among the 63 bean genotypes, followed by K (14.03–22.03 mg/g) and P (3.30–9.57 mg/g), with Mg (1.57–2.30 mg/g) and Ca (0.80–2.68 mg/g) concentrations exhibiting the least difference among the bean genotypes. Of the micronutrients, Fe levels revealed the highest variation (66.36–151.08 mg/kg), followed by Zn (23.57–70.72 mg/kg) and Mn (11.53–26.84 mg/kg), with B (10.06–17.65 mg/kg) and Cu (6.30–13.67 mg/kg) exhibiting relatively lower differences among the 63 common bean genotypes. However, genotype NUC 461 recorded the highest grain concentrations of P, K, Mg, Fe, Cu, Zn, and B, followed by DAB 155, which also revealed high levels of P, K, Ca, Fe, Zn, and Mn in its seeds. For improved human health and nutrition, the two bean genotypes would be the ideal candidates to recommend to commercial bean growers and resource‐poor farmers. However, the mechanisms underlying the greater accumulation of six to seven dietarily important nutrient elements by genotypes NUC 461 and DAB 155 remain to be determined.

ABSTRACT Cooking time (CT), post‐harvest darkening (PHD), and micronutrient content influence consumer preference of common bean (Phaseolus vulgaris L.) varieties. The objective of this study was to evaluate Andean genotypes for CT, PHD, and seed iron and zinc concentration. A total of 52 genotypes belonging to three market classes, namely, purple‐speckled (Kabulangeti), yellows, and cranberry (sugar beans), were grown at the Golden Valley Agricultural Research Trust (GART) Farm and the Mpika Research Farm in Zambia and assessed for CT, PHD, and seed iron and zinc concentration. The fastest cooking genotype among all 52 genotypes was a yellow genotype, Y1612‐5. Yellow genotypes generally cooked faster than the Kabulangeti and sugar types. Among the elite lines with Kabulangeti seed types, which is a dominant market class in Zambia, the elite line K175 had faster CT and higher iron and zinc concentration than the Kabulangeti landrace race currently available on the Zambian market. Kabulangeti and sugar genotypes showed regular darkening, whereas yellows did not darken. The yellow genotype Y1612‐3 was particularly outstanding because of its unique combination of high concentrations of both iron and zinc. Therefore, it can be used to introgress these two essential minerals into variable genetic backgrounds. The significant variability and high to moderate heritability for CT (0.85) and iron (0.81) and zinc (0.58) concentration observed in the current study suggest that it is possible to make progress in breeding for faster cooking varieties that are rich in iron and zinc.

Anthracnose (ANT), caused by Colletotrichum lindemuthianum, is the most devastating disease affecting common bean (Phaseolus vulgaris L.), leading to significant yield losses in the Western Himalayas. The study provides a comprehensive understanding of ANT resistance via trait phenotyping, biochemical profiling, genome-wide association studies (GWASs), and RNA sequencing. The assessment of bean association mapping panel in different environments revealed a diverse spectrum of resistance levels. Biochemical analysis revealed distinctive defense responses against ANT infection among different genotypes. GWAS approach identified 24 significant marker‒trait associations (MTAs) distributed across all 11 bean chromosomes. Notably, 03 MTAs (BMr205, BMr269 and BMr244) present on chromosome Pv07 were validated for ANT, and the remaining MTAs were novel MTAs for ANT. Transcriptome sequencing of resistant (PBG-3) and susceptible (PBG-26) genotypes under mock and 120-hour post inoculation conditions revealed key differentially expressed genes, such as leucine-rich repeat domain-containing protein (PHAVU_007G087700g), NB-ARC domain-containing protein (PHAVU_003G002500g) and transcription factors pivotal for disease resistance. The expression patterns of four genes (PHAVU_007G087700g, PHAVU_003G002500g, PHAVU_007G056100g and PHAVU_003G003000g) were validated through quantitative reverse transcription polymerase chain reaction (qRT‒PCR). Furthermore, the integration of GWAS-identified candidate genes with transcriptomics and cross-referencing with previous studies validated overlapping regions and common candidate genes, enriching our understanding of the genetic basis of ANT resistance. Therefore, the results offer a holistic perspective on ANT resistance in common bean, providing a foundation for targeted breeding efforts. The identified potential candidate genes and associated pathways will contribute valuable insights into the development of ANT resistant common bean varieties.

Field production of common beans benefit from root microbial associations, although they are generally considered to be weaker nitrogen fixers than other legumes. Therefore, the number of nodules on the roots of 64 accessions of the European common bean (Phaseolus vulgaris L.) and the relationship between the number of nodules per accession and other plant characteristics (growth type, earliness, seed yield, and total nitrogen content in seeds) were studied. The results indicated that growth type and earliness influenced the number of nodules per accession (the contributions of principal component analysis were 34.9% for Dim1 and 29.8% for Dim2). The average number of nodules per accession with indeterminate growth type was almost five times higher (20.1) than for accessions with determinate growth type (4.4). Common bean accessions with regular growth cycle length had the highest number of nodules (21.9). In contrast, nodulation efficiency, measured as seed yield per plant and total nitrogen content in seeds, were not correlated with the number of nodules per accession (correlation analysis, r

Salt stress is a major environmental abiotic stress factor. Plants sense salt from germination onwards, negatively affecting their growth and development. Enhancing salt tolerance in crops at the sprout stage is crucial, given that it is the first stage to encounter stress. Melatonin (N-acetyl-5-methoxytryptamine) is a potent antioxidant that can alleviate stress from various environmental factors. Here, a common bean variety “Heiyundou” was used as the plant material. A concentration of 70 mMol·L−1 NaCl was chosen as the stress treatment, and 100 μmol·L−1 melatonin was applied. Four treatment groups were established: CK (control, water treatment), S (salt stress), M (melatonin), and M+S (salt stress with melatonin). Melatonin application under salt stress (M+S) significantly improved sprout length, surface area, volume, and average diameter compared to the salt stress group (S). Physiological analysis revealed that salt stress increased the activity of reactive oxygen species (ROS) scavenging enzymes, while exogenous melatonin (M+S) further enhanced this activity. Salt stress also significantly elevated levels of stress markers like malondialdehyde (MDA), hydrogen peroxide (H2O2), and superoxide anion (O2−). However, these markers decreased under the M+S treatment, indicating melatonin's protective effect. RNA sequencing (RNA-Seq) analysis identified 639 differentially expressed genes (DEGs) between the control (W) and salt stress (S) groups, and 170 DEGs between the salt stress (S) and salt stress with melatonin (M+S) groups. 40 DEGs were common to both comparisons (Co-DEGs). Gene Ontology (GO) enrichment analysis revealed that oxidoreductase activity (GO:0016491) and oxidation–reduction processes (GO:0055114) were enriched in all three groups (WvsS, SvsM+S, and Co-DEGs). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that phenylpropanoid biosynthesis (Ko00940) was the most enriched pathway in all three groups. Within this pathway, 4-coumarate-CoA ligase (4CL) and peroxidase (POD) were identified as key enzymes. Molecular docking simulations further confirmed the binding potential of melatonin to these two enzymes. Additionally, 4CL activity and lignin content analyses supported the role of phenylpropanoid biosynthesis as the underlying mechanism of melatonin's protective action. Collectively, these findings provide a theoretical basis for applying melatonin in enhancing salt tolerance in common bean crops.

Xanthomonas citri pv. fuscans (Xcf) and Xanthomonas phaseoli pv. phaseoli (Xpp) are responsible for the Common Bacterial Blight (CBB), a major common bean (Phaseolus vulgaris) disease. The pathogenicity of Xcf and Xpp is known to be dependent upon a functional Type III Secretion System (T3SS) allowing the injection of numerous bacterial Type III Effectors (T3Es) into plant cells. T3Es have been described as able to disrupt plant defence and manipulate plant metabolism.In this work we described the transcriptomic response of one susceptible (Flavert) and one resistant (Vezer) cultivars of P. vulgaris to the inoculation of the virulent strain Xcf CFBP4885 or its avirulent T3SS-defective hrcV mutant (CFBP13802).Leaves of both bean cultivars were infiltrated with water or bacterial suspensions. Inoculated leaves were sampled at 24 or 48 h post inoculation (hpi). The experiment was independently repeated three times for total RNA extraction and sequencing analysis. Library construction and total RNA sequencing were performed with BGISEQ-500 at Beijing Genomics Institute (BGI, Hong-Kong), generating an average of 24M of paired-end reads of 100bp per sample. FastQC was used to check reads quality. Mapping analyses were made using a quasi-mapping alignment from Salmon (version 1.2.1) against the Phaseolus vulgaris reference genome (version 2.1), revealing the expression profiles of 36,978 transcripts in leaf tissues.Fastq raw data and count files from 36 samples are available in the Gene Expression Omnibus (GEO) repository of the National Center for Biotechnology Information (NCBI) under the accession number GSE271236.This dataset is a valuable resource to investigate the role of T3Es in subverting the cellular functions of bean.

Pod shattering is an undesirable process leading to loss of harvestable yields. In the present study, we sought to undertake the first comprehensive phenotyping in 254 bean (Phaseolus vulgaris L.) genotypes for pod shattering including various mechanistic aspects as well as assess natural variation in the germplasm set for 16 seed physical traits including shattering score. There was substantial variability in 16 pod physical traits in the material. Significant diversity of the material in respect of pod traits was indicated by the broad range and coefficient of variation (CV) values. Using Random Impact Assessment (RIA), we found substantial variability in pod shattering score in common bean genotypes indicating significant diversity. Shattering score had a mean value of 6.098 with a range of 1.07 to 9.13. Highest shattering score was recorded in WB-6, WB-20-247, and N-7 while the lowest value for shattering score was recorded in WB-1129 and WB-216. Shattering score was negatively correlated with pod thickness (r = -0.698) followed by ventral/dorsal length ratio (r = -0.468) and positively correlated with breadth/thickness ratio (r = 0.599) and string % (r = 0.590). The principal component analysis (PCA) concentrated 86.91% variability in the first five principal components, and the first two PCs accounted for 55.62% of the total variation.

Abstract Background Disease can drastically impair common bean (Phaseolus vulgaris L.) production. Anthracnose, caused by the fungal pathogen Colletotrichum lindemuthianum (Sacc. and Magnus) Briosi and Cavara, is one of the diseases that are widespread and cause serious economic loss in common bean. Results Transcriptome analysis of the early response of common bean to anthracnose was performed using two resistant genotypes, Hongyundou and Honghuayundou, and one susceptible genotype, Jingdou. A total of 9,825 differentially expressed genes (DEGs) responding to pathogen infection and anthracnose resistance were identified by differential expression analysis. By using weighted gene coexpression network analysis (WGCNA), 2,051 DEGs were found to be associated with two resistance-related modules. Among them, 463 DEGs related to anthracnose resistance were considered resistance-related candidate genes. Nineteen candidate genes were coexpressed with three resistance genes, Phvul.001G243600, Phvul.001G243700 and Phvul.001G243800. To further identify resistance genes, 46 candidate genes were selected for experimental validation using salicylic acid (SA) and methyl jasmonate (MeJA). The results indicated that 38 candidate genes that responded to SA/MeJA treatment may be involved in anthracnose resistance in common bean. Conclusions This study identified 38 resistance-related candidate genes involved in the early response of common bean, and 19 resistance-related candidate genes were coexpressed with anthracnose resistance genes. This study identified putative resistance genes for further resistance genetic investigation and provides an important reference for anthracnose resistance breeding in common bean.

Hawaii is known for its diverse micro-climates, making the evaluation of varieties at different locations an important strategy to determine the best varieties for each climate zone. Demand for dry beans in Hawaii has been rising due to the increase in production of value-added goods made from legumes. Initial field trials in 2017 were conducted to determine the best sowing date for dry beans in Hawaii since there were no previous such determinations. Field trials were conducted between 2018 and 2021 to evaluate 24 varieties of chickpea (Cicer arietinum), 21 varieties of common bean (Phaseolus vulgaris), and 10 varieties of cowpea (Vigna unguiculata) for their suitability and yield variability under Hawaii’s micro-climates. Preliminary sowing date trials were conducted in 2017, and a variety of trials were conducted between 2018 and 2021; seven field trials were conducted, including two in each of Oahu, Maui, and Hawaii County, and one in Kauai County. The trials were conducted in a randomized complete block design (RCBD) with three replicates. For all the study sites, 20-20-20 NPK fertilizer was applied at 30, 13, and 25 kg/ha N-P-K, respectively. A drip irrigation system was used in all locations as supplemental irrigation. Irrigation was used when needed and turned off 2 weeks prior to harvest. The results showed highly significant (p < 0.01) differences in yield between the varieties of each legume crop. Highly significant (p < 0.01) differences in yield were also found between the study locations. There was a significant (p < 0.05) decline in yield by 28% and 45% in chickpea and by 32% and 43% in common bean when planted 1 and 2 months, respectively, after the optimal mid-February planting on Oahu and Maui County. A decline of 21% and 50% in chickpea and 30 and 48% in common bean was recorded when planted 1 and 2 months, respectively, after the optimal mid-March planting in Hawaii County. The study results lead to developing site-specific recommendations for varieties and planting dates from each of the legume crops for each county. However, more studies are needed to develop site-specific recommendations for the micro-climates within each county.

The issue of storage and protection of yilded seeds from storage pests is fundamental to seed science, the nutrition of people and animals as well as the maintenance of state reserves. During 2019-2021, in the experimental field of IASS "Obraztsov chiflik" - Rousse, in a long-term stationary fertilizer experiment, seeds of common bean variety Obraztsov chiflik 12 were obtained utilizing the following fertilization options: 1 - individual nitrogen fertilization; 2 - individual phosphorus fertilization; 3 - individual potassium fertilization; 4 – combined NP application; 5 – combined NK application; 6 – combined PK application; 7 – triple combination of NPK. An unfertilized control variant was maintained for comparison purposes. The study aims to entomologically evaluate seeds regarding damage by bean weevils after crop harvesting. Measurements of yield, 1000-seed weight, damaged seeds percentage, Index of infestation, as well as weight loss were taken. The combined application of NP demonstrated the greatest positive effect on the yield qualities of beans, however it lead to an intermediate position of the yield in terms of damage by bean weevil. The variant treated with combined NK fertilization resulted in the lowest percentage of damaged seeds and Index of infestation; it was followed by the variant with full mineral fertilization and the control variant. The individual application of N resulted in highest damaged seeds percentage as well as highest Index of infestation; it also ensured the highest weight loss on average for the studied period.

The performance of most crop genotypes varies significantly depending on their genotype–environment interactions. Providing information on high-yielding and well-adapted common bean genotypes in a given environment is crucial for small-holder farmers to boost their productivity. A field trial was conducted at the Gendo and Wara locations over two consecutive seasons to understand genotype–environment interactions on grain yield and to identify and select common bean genotypes that were better adapted and had high yield potential for production. Ten genotypes were evaluated for their performance using a randomized complete block design with three replications. The AMMI and GGE models were used to identify superior genotypes regarding their mean grain yield performance. The AMMI analysis of variance results showed that grain yield was significantly (p ≤ 0.05) influenced by genotype–environment interactions. This shows that genotypes varied in grain yield mean performance across environments, indicating the possibility of selecting suitable genotypes with specific and broad adaptability. The sum of squares factor indicated that grain yield variation was contributed by the genotype (61.0%), followed by the environment (29.80%) and the genotype–environment interaction effect (9.20%) of the total variation. The AMMI and GGE biplot analysis results showed that SER-119 and SER-125 were identified as the best genotypes regarding their mean grain yield performance. Additionally, the genotype selection index analysis result indicated that SER-119 and SER-125 were stable and consistently exhibited high mean performance in all evaluated settings. Therefore, SER-119 and SER-125 are prime candidates for production in various agroecologies suitable for common bean cultivation in Dawuro zones. The use of tools like GGE biplots and AMMI analysis helped visualize the genotype–environment interactions, aiding in the selection of appropriate genotypes for both specific and wide adaptability.

Mitigating the effects of climate stress on crops is important for global food security. The microbiome associated with plant roots, the rhizobiome, can harbor beneficial microbes that alleviate stress, but the factors influencing their recruitment are unclear. We conducted a greenhouse experiment using field soil with a legacy of growing switchgrass and common bean to investigate the impact of short-term drought severity on the recruitment of active bacterial rhizobiome members. We applied 16S rRNA and 16S rRNA gene sequencing for both crops and metabolite profiling for switchgrass. We included planted and unplanted conditions to distinguish environment- versus plant-mediated rhizobiome drivers. Differences in community structure were observed between crops and between drought and watered and planted and unplanted treatments within crops. Despite crop-specific communities, drought rhizobiome dynamics were similar across the two crops. The presence of a plant more strongly explained the rhizobiome variation in bean (17%) than in switchgrass (3%), with a small effect of plant mediation during drought observed only for the bean rhizobiome. The switchgrass rhizobiome was stable despite changes in rhizosphere metabolite profiles between planted and unplanted treatments. We conclude that rhizobiome responses to short-term drought are crop-specific, with possible decoupling of plant exudation from rhizobiome responses. In a study of short-term drought, the authors show an effect of plant mediation on the active rhizosphere microbiome of common bean, but not switchgrass. They conclude that microbiome responses are crop-specific and have possible decoupling of plant exudation and microbiome response. [ABSTRACT FROM AUTHOR]

Introduction: Genotyping large-scale gene bank collections requires an appropriate sampling strategy to represent the diversity within and between accessions. Methods: A panel of 44 common bean (Phaseolus vulgaris L.) landraces from the Alliance Bioversity and The Alliance of Bioversity International and the International Center for Tropical Agriculture (CIAT) gene bank was genotyped with DArTseq using three sampling strategies: a single plant per accession, 25 individual plants per accession jointly analyzed after genotyping (in silico-pool), and by pooling tissue from 25 individual plants per accession (seq-pool). Sampling strategies were compared to assess the technical aspects of the samples, the marker information content, and the genetic composition of the panel. Results: The seq-pool strategy resulted inmore consistent DNA libraries for quality and call rate, although with fewer polymorphic markers (6,142 single-nucleotide polymorphisms) than the in silico-pool (14,074) or the single plant sets (6,555). Estimates of allele frequencies by seq-pool and in silico-pool genotyping were consistent, but the results suggest that the difference between pools depends on population heterogeneity. Principal coordinate analysis, hierarchical clustering, and the estimation of admixture coefficients derived froma single plant, in silico-pool, and seq-pool successfully identified the well-known structure of Andean and Mesoamerican gene pools of P. vulgaris across all datasets. Conclusion: In conclusion, seq-pool proved to be a viable approach for characterizing common bean germplasm compared to genotyping individual plants separately by balancing genotyping effort and costs. This study provides insights and serves as a valuable guide for gene bank researchers embarking on genotyping initiatives to characterize their collections. It aids curators in effectively managing the collections and facilitates marker-trait association studies, enabling the identification of candidate markers for key traits. [ABSTRACT FROM AUTHOR]

Although seed represents an important means of plant pathogen dispersion, the seed–pathogen dialogue remains largely unexplored. A multiomic approach was performed at different seed developmental stages of common bean (Phaseolus vulgaris L.) during asymptomatic colonization by Xanthomonas citri pv. fuscans (Xcf), At the early seed developmental stages, we observed high transcriptional changes both in seeds with bacterial recognition and defense signal transduction genes, and in bacteria with up‐regulation of the bacterial type 3 secretion system. This high transcriptional activity of defense genes in Xcf‐colonized seeds during maturation refutes the widely diffused assumption considering seeds as passive carriers of microbes. At later seed maturation stages, few transcriptome changes indicated a less intense molecular dialogue between the host and the pathogen, but marked by changes in DNA methylation of plant defense genes, in response to Xcf colonization. We showed examples of pathogen‐specific DNA methylations in colonized seeds acting as plant defense silencing to repress plant immune response during the germination process. Finally, we propose a novel plant–pathogen interaction model, specific to the seed tissues, highlighting the existence of distinct phases during seed–pathogen interaction with seeds being actively interacting with colonizing pathogens, then both belligerents switching to more passive mode at later stages. [ABSTRACT FROM AUTHOR]

Background: Laccase (LAC) gene family plays a pivotal role in plant lignin biosynthesis and adaptation to various stresses. Limited research has been conducted on laccase genes in common beans. Results: 29 LAC gene family members were identified within the common bean genome, distributed unevenly in 9 chromosomes. These members were divided into 6 distinct subclades by phylogenetic analysis. Further phylogenetic analyses and synteny analyses indicated that considerable gene duplication and loss presented throughout the evolution of the laccase gene family. Purified selection was shown to be the major evolutionary force through Ka / Ks. Transcriptional changes of PvLAC genes under low temperature and salt stress were observed, emphasizing the regulatory function of these genes in such conditions. Regulation by abscisic acid and gibberellins appears to be the case for PvLAC3, PvLAC4, PvLAC7, PvLAC13, PvLAC14, PvLAC18, PvLAC23, and PvLAC26, as indicated by hormone induction experiments. Additionally, the regulation of PvLAC3, PvLAC4, PvLAC7, and PvLAC14 in response to nicosulfuron and low-temperature stress were identified by virus-induced gene silence, which demonstrated inhibition on growth and development in common beans. Conclusions: The research provides valuable genetic resources for improving the resistance of common beans to abiotic stresses and enhance the understanding of the functional roles of the LAC gene family. [ABSTRACT FROM AUTHOR]

Common bean (Phaseolus vulgaris L.) is an essential food staple and source of income for small-holder farmers across Africa. However, yields are greatly threatened by fungal diseases like root rot induced by Rhizoctonia solani. This study aimed to evaluate an integrated approach utilizing vermicompost tea (VCT) and antagonistic microbes for effective and sustainable management of R. solani root rot in common beans. Fourteen fungal strains were first isolated from infected common bean plants collected across three Egyptian governorates, with R. solani being the most virulent isolate with 50% dominance. Subsequently, the antagonistic potential of vermicompost tea (VCT), Serratia sp., and Trichoderma sp. was assessed against this destructive pathogen. Combinations of 10% VCT and the biocontrol agent isolates displayed potent inhibition of R. solani growth in vitro, prompting in planta testing. Under greenhouse conditions, integrated applications of 5 or 10% VCT with Serratia marcescens, Trichoderma harzianum, or effective microorganisms (EM1) afforded up to 95% protection against pre- and post-emergence damping-off induced by R. solani in common bean cv. Giza 6. Similarly, under field conditions, combining VCT with EM1 (VCT + EM1) or Trichoderma harzianum (VCT + Trichoderma harzianum) substantially suppressed disease severity by 65.6% and 64.34%, respectively, relative to untreated plants. These treatments also elicited defense enzyme activity and distinctly improved growth parameters including 136.68% and 132.49% increases in pod weight per plant over control plants. GC–MS profiling of Trichoderma harzianum, Serratia marcescens, and vermicompost tea (VCT) extracts revealed unique compounds dominated by cyclic pregnane, fatty acid methyl esters, linoleic acid derivatives, and free fatty acids like oleic, palmitic, and stearic acids with confirmed biocontrol and plant growth-promoting activities. The results verify VCT-mediated delivery of synergistic microbial consortia as a sustainable platform for integrated management of debilitating soil-borne diseases, enhancing productivity and incomes for smallholder bean farmers through regeneration of soil health. Further large-scale validation can pave the adoption of this climate-resilient approach for securing food and nutrition security. [ABSTRACT FROM AUTHOR]

The common bean (Phaseolus vulgaris L.) is an economically important food crop grown worldwide; however, its production is affected by various environmental stresses, including cold, heat, and drought stress. The plant U-box (PUB) protein family participates in various biological processes and stress responses, but the gene function and expression patterns of its members in the common bean remain unclear. Here, we systematically identified 63 U-box genes, including 8 tandem genes and 55 non-tandem genes, in the common bean. These PvPUB genes were unevenly distributed across 11 chromosomes, with chromosome 2 holding the most members of the PUB family, containing 10 PUB genes. The analysis of the phylogenetic tree classified the 63 PUB genes into three groups. Moreover, transcriptome analysis based on cold-tolerant and cold-sensitive varieties identified 4 differentially expressed PvPUB genes, suggesting their roles in cold tolerance. Taken together, this study serves as a valuable resource for exploring the functional aspects of the common bean U-box gene family and offers crucial theoretical support for the development of new cold-tolerant common bean varieties. [ABSTRACT FROM AUTHOR]

Introduction: Effective weed management tools are crucial for maintaining the profitable production of snap bean (Phaseolus vulgaris L.). Preemergence herbicides help the crop to gain a size advantage over the weeds, but the few preemergence herbicides registered in snap bean have poor waterhemp (Amaranthus tuberculatus) control, a major pest in snap bean production. Waterhemp and other difficult-to-control weeds can be managed by flumioxazin, an herbicide that inhibits protoporphyrinogen oxidase (PPO). However, there is limited knowledge about crop tolerance to this herbicide. We aimed to quantify the degree of snap bean tolerance to flumioxazin and explore the underlying mechanisms. Methods: We investigated the genetic basis of herbicide tolerance using genome-wide association mapping approach utilizing field-collected data from a snap bean diversity panel, combined with gene expression data of cultivars with contrasting response. The response to a preemergence application of flumioxazin was measured by assessing plant population density and shoot biomass variables. Results: Snap bean tolerance to flumioxazin is associated with a single genomic location in chromosome 02. Tolerance is influenced by several factors, including those that are indirectly affected by seed size/weight and those that directly impact the herbicide's metabolism and protect the cell from reactive oxygen species-induced damage. Transcriptional profiling and co-expression network analysis identified biological pathways likely involved in flumioxazin tolerance, including oxidoreductase processes and programmed cell death. Transcriptional regulation of genes involved in those processes is possibly orchestrated by a transcription factor located in the region identified in the GWAS analysis. Several entries belonging to the Romano class, including Bush Romano 350, Roma II, and Romano Purpiat presented high levels of tolerance in this study. The alleles identified in the diversity panel that condition snap bean tolerance to flumioxazin shed light on a novel mechanism of herbicide tolerance and can be used in crop improvement. [ABSTRACT FROM AUTHOR]

Introduction: Drought is one of the biggest problems for crop production and also affects the survival and persistence of soil rhizobia, which limits the establishment of efficient symbiosis and endangers the productivity of legumes, the main source of plant protein worldwide. Aim: Since the biodiversity can be altered by several factors including abiotic stresses or cultural practices, the objective of this research was to evaluate the effect of water availability, plant genotype and agricultural management on the presence, nodulation capacity and genotypic diversity of rhizobia. Method: A field experiment was conducted with twelve common bean genotypes under irrigation and rain-fed conditions, both in conventional and organic management. Estimation of the number of viable rhizobia present in soils was performed before the crop establishment, whereas the crop yield, nodule number and the strain diversity of bacteria present in nodules were determined at postharvest. Results: Rainfed conditions reduced the number of nodules and of isolated bacteria and their genetic diversity, although to a lesser extent than the agrochemical inputs related to conventional management. In addition, the effect of water scarcity on the conventional management soil was greater than observed under organic conditions. Conclusions: The preservation of diversity will be a key factor to maintain crop production in the future, as problems caused by drought will be exacerbated by climate change and organic management can help to maintain the biodiversity of soil microbiota, a fundamental aspect for soil health and quality. [ABSTRACT FROM AUTHOR]

The objective of this study was to study the physiological quality of bean treated with levels of zinc during the physiological maturation stage. The work was carried out at Fazenda Ponte da Braúna and at the Seed Analysis Laboratory of the Center for Agricultural Sciences and Engineering at the Federal University of Espírito Santo. Bean seeds of the cultivar Peanut were treated with Zn at concentrations of 0; 2.5; 5.0; 7.5 and 10 g kg-1 of seeds and sown in the field. The variables analyzed were seed moisture, 1000-seed weight, length, width and thickness of pods and seeds, germination, germination speed index, shoot length, root length, shoot and root dry matter. The experimental design used was completely randomized, in the factorial 5 x 7 (zinc doses x maturation periods), with four replications of 25 seeds. The seeds of the bean cultivar Amendoim treated with zinc at 10 g kg-1 seeds showed the highest values of pod length, thickness, and width. The seeds of the bean cultivar Amendoim showed the highest germination means and GSI after 35 days from anthesis. The treatment of seeds of the bean cultivar Amendoim with zinc does not influence the seedling characteristics. Seed treatment with zinc does not interfere with the seed maturation time of the bean cultivar Amendoim. The physiological maturation of seeds of the bean cultivar Amendoim occurs after 42 days from anthesis. The seed water content of the bean cultivar Amendoim at the stage of physiological maturation is 19%. [ABSTRACT FROM AUTHOR]

Wild crop germplasm and landrace varieties have long been considered as a source of novel genetic variation for improving domesticated crops. Due to genetic bottlenecks that occurred during domestication, dissemination, and subsequent crop improvement, many beneficial genes are absent from commercial varieties and elite breeding germplasm, yet are still present in wild populations. To catalog potential novel disease resistance genes useful for breeding more resilient dry beans, we screened wild and landrace Phaseolus vulgaris L. accessions archived in the USDA GRIN seed bank for untapped resistance to anthracnose (Colletotrichum lindemuthianum) and bean common mosaic necrosis virus (BCMNV). A population of 194 wild and 55 landrace accessions were screened for resistance to two highly virulent races of anthracnose (race 2047 and race 3481) and for resistance to the BCMNV strain NL‐3. Thirteen accessions, all wild, were resistant to both anthracnose races, with MX‐QT‐3 and PI 661812 displaying complete resistance with no disease symptoms across all replications. Surprisingly, no natural resistance to BCMNV strain NL‐3 was detected in the wild germplasm. PI 442541, PI 661891, and W6 10164 were the only three accessions that expressed the I‐gene phenotype and all three have some history of cultivation. This study provides a resource for introgression of genetic diversity for anthracnose resistance into cultivated beans and reveals that innate resistance to BCMNV may not exist in wild populations of P. vulgaris. Core Ideas: Wild Phaseolus vulgaris germplasm has genetic and phenotypic diversity not present in cultivated varieties.Resistance to two races of anthracnose exists in wild bean germplasm.Resistance to BCMNV NL‐3 was not found in wild bean germplasm, but in three landraces. [ABSTRACT FROM AUTHOR]

Drought stress (DS) is one of the main environmental stresses that determines crop productivity. It has been estimated that DS depresses over 40%–60% of soybean (Glycine max (L.) Merr.) and common bean (Phaseolus vulgaris L.) production worldwide, respectively. Although different agronomic strategies are sometimes implemented, the current goal in sustainable agriculture could involve the inoculation with native microorganisms to mitigate DS effects. A potential fungal candidate is Trichoderma, which is recognized as a ubiquitous soil inhabitant with growth‐promoting and biocontrol potentiality. However, its potential for mitigating the stress for water deficit is less well‐documented. Our objective was to evaluate the effect of inoculation with native Trichoderma harzianum strains on soybean and common bean growth under contrasting conditions of water availability. Seeds were independently inoculated (or not) with IB‐J15 and IB‐363 strains, and plants were submitted to DS or were kept under optimal irrigation (well‐watered, WW). In both legumes, the most evident effect after being inoculated was the modification of plant root architecture, the increase in root area and the development of lateral roots in plants under WW and DS conditions. In soybean, both Trichoderma strains had a positive inoculation response, both fresh and dry root biomass increased under WW, and remarkably under DS conditions. The main effect was an increase of about 110% in root dry weight under WW and, about 330% in DS in plants inoculated with IB‐J15 strain, meanwhile, plants inoculated with IB‐363 increased root dry weight 60% in WW and 177% in DS conditions. Notably in soybean, the inoculation with both Trichoderma strains increased the root area more than 70% in both WW and DS conditions. Common beans inoculated with IB‐363 under WW conditions, reached a positive inoculation responsiveness of around 247% in shoot dry weight biomass, and under WW both strains increased the root area more than 50%. Further, IB‐363 increased leaf area by 25% in WW and 72% in DS. Additionally, the in vitro co‐culture between both Trichoderma strains and nodulating Rhizobium etli and Bradyrhizobium japonicum E109 showed compatibility between microorganisms, since no inhibition of their growth was observed. We emphasize that plants inoculated with Trichoderma showed better resistance to water deficit, as seen by redistribution of photosynthates, prioritizing mainly, the development of the root system. © 2024 Association of Applied Biologists. [ABSTRACT FROM AUTHOR]

Simple Summary: Bean leaf beetles (BLBs) are significant pests in Uganda, damaging crops such as beans and cowpeas, leading to substantial yield losses. They exhibit preferential feeding behaviour, targeting specific crops over others. Understanding these preferences can form the basis for identifying a potential trap crop that can be used to manage the pest at a low cost in a sustainable manner. A field study was conducted to determine the feeding preference of BLBs on various host crops among those commonly cultivated in Uganda, across different locations and seasons. This study was conducted in Arua and Lira districts in the first and second rainy seasons of 2018. Seven BLB host crops, i.e., common bean, cowpea, greengram, soybean, groundnuts, okra and roselle (locally known as malakwang), were selected for the study. The results showed that cowpea exhibited the highest abundance of BLBs among all crops and it had a high amount of foliar damage as well. Thus, it was selected as the most preferred host crop and can be recommended as a trap crop for managing Ootheca spp. With respect to locations and seasons, the pest was more abundant in Arua than in Lira and more abundant in 2018A than 2018B across all locations. The bean leaf beetle (BLB) (Ootheca spp.) is a polyphagous pest causing significant yield losses in Uganda, particularly in the Northern and Eastern regions on various hosts plants. Despite its polyphagous behaviour, the BLB exhibits preferential feeding, offering an opportunity for targeted pest management. This study explored its feeding preferences across seven crops: common bean, cowpea, greengram, okra, roselle (malakwang), groundnuts, and soybean. This study was conducted in Arua and Lira districts using a randomized complete block design for two rainy seasons (2018A and 2018B). The results showed significant differences in BLB abundance and foliar damage among host crops, locations, days after planting and seasons. Cowpea was the most preferred crop while groundnuts was the least preferred. Therefore, cowpea can be recommended for use as a trap for managing Ootheca spp. in gardens where it is not the main crop. There was a higher pest abundance in Arua than in Lira. There was also a higher pest abundance in 2018A than in 2018B. These findings highlight the importance of understanding BLB's feeding preferences for implementing effective IPM strategies, emphasizing the potential role of trap cropping, especially with cowpea, to minimize BLB damage in resource-constrained agricultural settings. [ABSTRACT FROM AUTHOR]

Snap bean (Phaseolus vulgaris L.) is among the leading vegetables in Kenya that is mainly grown for export with the potential to increase household income. There are few programs in Kenya that focus on breeding new cultivars for increased production. The development of snap bean varieties that are resilient to the changing climate is crucial for sustainable agriculture in Kenya. To achieve this, knowledge of gene action, trait expression, and heritability is vital in effective breeding for quantitative traits like pod quality and yield. A study was conducted to investigate the inheritance of pod quality and yield traits in snap beans, which can inform the breeding of snap beans. A half diallel cross involving two indeterminate dry beans (G2333 and MCM 5001) and five determinate snap beans (Amy, Moonstone, Seagull, Serengeti and Vanilla) varieties was conducted. Data on pod traits (pod weight per plant, pod number per plant, pod length, pod diameter, pod wall fiber and pod suture string) were collected from 21 F1s and 7 parents grown in two locations (Embu and Kirinyaga Counties) in Kenya. The results showed significant genotypic and environmental effects (P < 0.001) for all traits, with significant genotype by environment (G × E) interactions for most traits. Additionally, general combining abilities (GCA) and specific combining abilities (SCA) were significant for all traits evaluated. The significance of GCA and SCA indicated the importance of both additive and non-additive gene effects in controlling the traits, although the additive gene effects were predominant. The study revealed that Vanilla and Serengeti had the highest GCA estimate for pod quality traits, while G2333 and MCM5001 had the highest GCA estimate for pod yield traits. Snap bean pod yield and quality are quantitative traits controlled by multiple genes and influenced by the environment. Therefore, the predominance of additive gene effects suggests that selection for these traits in segregating generations could yield satisfactory gains. The results of this study could impact the development of snap bean varieties that are more resilient to the effects of climate change. Thus, improving the sustainability and productivity of the agriculture sector in Kenya. [ABSTRACT FROM AUTHOR]

Common beans (Phaseolus vulgaris L.), besides being an important source of nutrients such as iron, magnesium, and protein, are crucial for food security, especially in developing countries. Common bean cultivation areas commonly face production challenges due to drought occurrences, mainly during the reproductive period. Dry spells last approximately 20 days, enough time to compromise production. Hence, it is crucial to understand the genetic and molecular mechanisms that confer drought tolerance to improve common bean cultivars' adaptation to drought. Sixty six RNASeq libraries, generated from tolerant and sensitive cultivars in drought time sourced from the R5 phenological stage at 0 to 20 days of water deficit were sequenced, generated over 1.5 billion reads, that aligned to 62,524 transcripts originating from a reference transcriptome, as well as 6673 transcripts obtained via de novo assembly. Differentially expressed transcripts were functionally annotated, revealing a variety of genes associated with molecular functions such as oxidoreductase and transferase activity, as well as biological processes related to stress response and signaling. The presence of regulatory genes involved in signaling cascades and transcriptional control was also highlighted, for example, LEA proteins and dehydrins associated with dehydration protection, and transcription factors such as WRKY, MYB, and NAC, which modulate plant response to water deficit. Additionally, genes related to membrane and protein protection, as well as water and ion uptake and transport, were identified, including aquaporins, RING-type E3 ubiquitin transferases, antioxidant enzymes such as GSTs and CYPs, and thioredoxins. This study highlights the complexity of plant response to water scarcity, focusing on the functional diversity of the genes involved and their participation in the biological processes essential for plant adaptation to water stress. The identification of regulatory and cell protection genes offers promising prospects for genetic improvement aiming at the production of common bean varieties more resistant to drought. These findings have the potential to drive sustainable agriculture, providing valuable insights to ensure food security in a context of climate change. [ABSTRACT FROM AUTHOR]

Sclerotinia sclerotiorum is an economically damaging fungal pathogen that causes Sclerotinia stem rot in leguines, producing enormous yield losses. This pathogen is difficult to control due to its wide host spectrum and ability to produce sclerotia. which are resistant bodies that can remain active for long periods under harsh environmental conditions. Here, the biocontrol methods for the management of S. sclerotiorum in legumes are reviewed. Baciltus strains, which synthesized lipopeptides and volatile organic compounds, showed high efficacies in soybean plants, whereas the highest efficacies for the control of the pathogen in alfalfa and common bean were observed when using Coniothyrium minitans and Streptomyces spp., respectively. The biocontrol efficacies in fields were under 65%, highlighting the lack of strategies to achieve a complete control. Overall, although most studies involved extensive sci·eenings using different biocontrol agent concentrations and application conditions, there is a lack of knowledge regarding the specific anti fungal mechanisms, which limits the optimization of the reported methods. [ABSTRACT FROM AUTHOR]

The purpose of this present study is to explore and decipher copper tolerant plant growth promoting bacteria from dolomite contaminated soil. Lysinibacillus sphaericus (NJCT1) isolated from tea garden soil near Kaljani river bank has been found to exhibit multifaceted characteristics and has shown its ability to tolerate copper up-to 40 mg/ml. Further, its ability to tolerate other heavy metals were also assessed and the result revealed that it can tolerate iron, zinc and aluminum upto 100 mg/ml, while for lead and arsenic it showed tolerance upto 10 mg/ml. GC-MS analysis showed that L. sphaericus (NJCT1) produced 16 compounds, among them, tromethamine, 2-methylbutanoic acid, 2,4,6-(1H,3H,5H)-pyrimidinetrione, 2-piperidinone, 3,5,5-trimethylhexyl acetate and cis-3-hexenyl propionate were found to exhibit various important biological properties. Also, this isolate was positive for phosphate solubilization and is capable of producing ammonia, HCN, siderophore, and 17.12 µg/ml of indole acetic acid. In seed germination, seeds treated with NJCT1 demonstrated 100% germination rate with 111-time increase in seedling vigor index. Further, in pot study, significant increase in all the growth parameters of NJCT1 treated Vigna radiata and Phaseolus vulgaris gave a strong indication that this strain can be a promising candidate for reclamation and revitalization of dolomite and copper ore affected tea garden soil. [ABSTRACT FROM AUTHOR]

MADS-box genes are known to play important roles in diverse aspects of growth/devolopment and stress response in several plant species. However, no study has yet examined about MADS-box genes in P. vulgaris. In this study, a total of 79 PvMADS genes were identified and classified as type I and type II according to the phylogenetic analysis. While both type I and type II PvMADS classes were found to contain the MADS domain, the K domain was found to be present only in type II PvMADS proteins, in agreement with the literature. All chromosomes of the common bean were discovered to contain PvMADS genes and 17 paralogous gene pairs were identified. Only two of them were tandemly duplicated gene pairs (PvMADS-19/PvMADS-23 and PvMADS-20/PvMADS-24), and the remaining 15 paralogous gene pairs were segmentally duplicated genes. These duplications were found to play an important role in the expansion of type II PvMADS genes. Moreover, the RNAseq and RT-qPCR analyses showed the importance of PvMADS genes in response to drought stress in P. vulgaris. [ABSTRACT FROM AUTHOR]

Surveys were conducted in five districts of Nagaland to collect crop legumes grown in Jhum fields and home gardens. Ten crop legumes, namely Canavalia gladiata (Jacq.) DC, Glycine max (L.) Merr, Lablab purpureus L., Mucuna prurita (L.) DC, Phaseolus lunatus L., Phaseolus vulgaris L., Psophocarpus tetragonolobus L., Vicia faba (L.) D.C, Vigna umbellata (Thunb.) Ohwi and H. Ohashi and Vigna unguiculata (L.) Walp were commonly cultivated. Vigna unguiculata and Vigna umbellata were the most popularly grown beans. Root nodule was observed in all nine crop legumes except for Vicia faba. The nodules were of the determinate type. Number of root nodules and nodule size varies with the species. Large nodules were found in Canavalia gladiata, Psophocarpus tetragonolobus, and Mucuna prurita with the mean size of 5.15 ± 0.617 mm, 4.93 ± 0.115 mm, and 4.83 ± 0.289 mm. The smallest was in Vigna umbellata, with 1.45 ± 0.610 mm. Root nodule endophytes belonging to Rhizobium sp. were isolated from three popular crop legumes, namely, V. umbellata, V. unguiculata, and P. vulgaris. The study underscores the importance of further research on the crop legume symbiotic association with native root nodulating bacteria (rhizobia) for crop productivity. [ABSTRACT FROM AUTHOR]

Protected geographical indications (PGIs) share health problems related to plant propagation material. The PGI "Faba de Lourenzá" encompasses a 1660 km2 area in northern Galicia, Spain, renowned for its "Faba Galaica" (FG) and Faba do marisco" (FM) bean cultivars. The lack of certified virus-free seeds poses a challenge. From 2019 to 2023, seeds from 60 lots were tested for BCMV. Plants from several plots were tested periodically to develop disease progress curves (DPCs). Control methods (plots out PGI zone, virus-free seedlings, rogueing, corn borders, and intercropping) were tested. Yields in five plots were used to assess BCMV's economic impact. Seed lots were 22.3% FG-infected and <5% FM-infected. The transmission rate of BCMV from infected FG plants to their seeds was 25.5 ± 5%, while for FM it was 12 ± 3%. FG yield losses were on average 31.6 ± 4.5%. Combining virus-free seedlings and infected plant removal in plots outside the PGI area proved effective at reducing infection rates; combining with intercropping resulted in the lowest incidence in an FG plot. Farmer training and off-site plot selection to produce healthy sowing beans are key to improving results. [ABSTRACT FROM AUTHOR]

Deficit irrigation (DI) strategies are becoming increasingly common in areas where water resources are limited. The application of moderate levels of DI can result in water savings with a small reduction in yield but with a higher quality of the product. The aim of this work was to evaluate the effect of applying a certain level of water deficit (40% water holding capacity) on the yield and quality of the common bean (Phaseolus vulgaris L.), specifically the cultivar 'Triunfo-70'. Bioactive compounds were investigated by applying an LC-MS-based untargeted metabolomics approach as an analytical tool for identifying novel markers associated with a water deficit in beans. The results showed that beans harvested 30 days after DI application experienced water stress, as indicated by the decrease in the leaf water potential and gas exchange values (stomatal conductance and photosynthesis). In addition, the number of pods per plant was significantly reduced by the DI treatment. The water deficit induced significant alterations in various bioactive compounds (including organic acids, polyphenols, hydroxybenzoic acids, lipids, and phospholipids) when compared to the control treatment. Additionally, twelve new biomarkers were identified in this study for the first time in the common bean under DI. These findings suggested that DI acted as an elicitor, increasing phenylpropanoid metabolism, while concurrently reducing the production of compounds associated with fatty acid metabolism. Additionally, new metabolites were tentatively identified in common beans. This study represents the successful application of the untargeted metabolomics approach to finding bioactive secondary metabolites in beans under different irrigation conditions. [ABSTRACT FROM AUTHOR]

Beans usually have similar physical attributes; thus, it is difficult to distinguish them manually. Size, shape, and mass attributes of seeds help in breeding, selection, classification, separation, and machine design. This study was conducted to determine physical attributes of 20 bean genotypes with the use of image processing techniques. Color characteristics of the present genotypes were also determined. Then, four different machine learning algorithms (MLP, RF, SVR, and k-NN) were employed to predict seed mass. Among the present genotypes, Güzelöz and Özdemir genotypes had the highest size, shape, and color characteristics. Highly significant positive correlations were encountered between projected area-equivalent diameter (r = 1.00), between geometric mean diameter—surface area and volume (r = 1.00). On the other hand, highly significant negative correlations were seen between sphericity—elongation in vertical orientation (r = − 0.98). In hierarchical cluster analysis for physical attributes, Alberto–Aslan and Aras 98–Şahin genotypes were identified as the closest genotypes. According to PCA analysis, the first two principal components (PC1 and PC2) were able to explain 73% of total variation among the genotypes. While PC1 axis included projected area (vertical), equivalent diameter (vertical), and length, PC2 axis included L*, a*, b*, sphericity, roundness (vertical), and elongation (vertical). Among the present machine learning algorithms, RF yielded the best performances in mass estimation of bean seeds. It was concluded that machine learning techniques increased the efficiency of related machinery and helped to save time and labor. [ABSTRACT FROM AUTHOR]

This study showcases a promising approach to sustainably unlocking plant biomass residues by combining biodegradation with artificial intelligence to optimize the process. Specifically, we utilized the definitive screening design (DSD) and artificial neural networks (ANNs) to optimize the degradation of common bean biomass by the endophytic fungus Trichoderma asperellum WNZ-21. The optimized process yielded a fungal hydrolysate rich in 12 essential and non-essential amino acids, totaling 18,298.14 μg/g biomass. GC-MS analysis revealed four potential novel components not previously reported in microbial filtrates or plants and seven components exclusive to plant sources but not reported in microbial filtrates. The hydrolysate contained phenolic, flavonoid, and tannin compounds, as confirmed by FT-IR analysis. High-resolution transmission electron microscopy depicted structures resembling amino acid micelles and potential protein aggregates. The hydrolysate exhibited antioxidant, antibacterial, and anticancer properties and innovatively induced apoptotic modulation in the MCF7 cancer cell line. These findings underscore the potential of ANN-optimized fermentation for various applications, particularly in anticancer medicine due to its unique composition and bioactivities. The integration of the DSD and ANNs presents a novel technique for biomass biodegradation, warranting the valorization of plant biomass and suggesting a further exploration of the new components in the fungal hydrolysate. This approach represents the basic concept for exploring other biomass sources and in vivo studies. [ABSTRACT FROM AUTHOR]