Search

The development of a facile cysteine-directed S-glycosylation strategy would facilitate the intensive investigation of the effect of glycosylation on protein translational modification. Herein, we ...

Background Bicarbonate Ringer’s (BR) solution is a direct, liver and kidney metabolism-independent HCO3− buffering system. We hypothesized that BR solution is more effective in acid-base equilibrium and more conducive to better liver function than acetate Ringer’s (AR) solution in liver transplantation (LT).Methods Sixty-nine adult patients underwent orthotopic LT. Patients in the bicarbonate and acetate groups received BR and AR solutions, respectively. The primary outcome was the effect on pH and BE levels. The secondary outcome measures were the intraoperative 5% sodium bicarbonate infusion incidence and volume and liver and kidney function laboratory indicators.Results The pH and absolute BE values changed significantly during the anhepatic stage and immediately after transplanted liver reperfusion in the bicarbonate group compared with the acetate group (all P P P Conclusion Compared with AR solution, BR solution was associated with improved intraoperative acid-base balance, protected early postoperative liver graft function and reduced late-postoperative renal injury.

Supplementary Table from O-GlcNAcylation of Blimp-1 in Lymphocytes Inhibits Its Transcriptional Function and Is Associated with Migration and Invasion of Breast Cancer Cells

Supplementary Data from O-GlcNAcylation of Blimp-1 in Lymphocytes Inhibits Its Transcriptional Function and Is Associated with Migration and Invasion of Breast Cancer Cells

Lymphocyte infiltration is an important feature of cancer. There is a complex network of chemokines that influence the degree and phenotype of lymphocyte infiltration, as well as the growth, survival, migration, and angiogenesis of tumor cells. High heterogeneity metastasis is a major obstacle to the treatment of breast cancer. Herein, we showed that O-GlcNAcylation of B lymphocyte–induced maturation protein-1 (Blimp-1) in lymphocytes inhibited the migration and invasion of breast cancer cells. It was found that Blimp-1 O-GlcNAcylation at Ser448 and Ser472 in lymphocytes promoted its nuclear localization, and blocked the bindings to three regions upstream of the ccl3l1 promoter to inhibit its expression. Decreased expression of CCL3L1 in lymphocytes not only decreased CCR5 expression in breast cancer cells, but also inhibited the membrane localization and activation of CCR5, thus blocking the migration and invasion of breast cancer cells in vitro. Therefore, O-GlcNAcylation of Blimp-1 in lymphocytes may serve as a new target for the treatment of metastatic breast cancer.Implications:This study reveals a new mechanism by which the lymphatic system promotes breast cancer cell metastasis.

Supplementary Figure from O-GlcNAcylation of Blimp-1 in Lymphocytes Inhibits Its Transcriptional Function and Is Associated with Migration and Invasion of Breast Cancer Cells

In the hydraulic fracturing process of shale reservoir, proppant will embed and deform under the action of high closure stress. Thus, it is necessary to analyze the mechanical process and influencing factors of proppant embedding in shale. This study reproduced rough fracture surface of real rock slabs based on the reverse reconstruction method and obtained the mechanical parameters of the rock slab and proppant. In this study, a numerical model for the elastoplastic deformation of the proppant embedding in the rough fracture surface, along with mechanical test experiments, is proposed. The reliability of the numerical model is verified by the proppant embedding simulation experiment. Based on this model, the process of proppant embedding in a rough fracture under the action of closing stress and the factors influencing the width of supporting fracture were analyzed. The results show that the embedment degree of the proppant is different in different areas of the rough fracture surface. Furthermore, a stress concentration effect is apparent. The proppant embedment becomes significant after the slab enters the plastic deformation stage under high closure stress. The slabs with high roughness and low Young's modulus or proppants with small particle sizes and high Young's moduli result in a smaller width of the propped fracture.

Triplet excitons usually do not emit light under ambient conditions due to the spin-forbidden transition rule, thus they are called dark excitons. Herein, triplet excitons in carbon nanodots (CNDs) are brightened by embedding the CNDs into poly(vinyl alcohol) (PVA) films; flexible multicolor phosphorescence films are thus demonstrated. PVA chains can isolate the CNDs, and excited state electron or energy transfer induced triplet exciton quenching is thus reduced; while the formed hydrogen bonds between the CNDs and PVA can restrict vibration/rotation of the CNDs, thus further protecting the triplet excitons from nonradiative recombination. The lifetimes of the flexible multicolor phosphorescence films can reach 567, 1387, 726, and 311 ms, and the longest-lasting phosphorescence film can be observed by naked eyes for nearly 15 s even after bending 5000 times. The phosphorescence films can be processed into various patterns, and a dynamic optical signature concept has been proposed and demonstrated based on the phosphorescence films.

Dense glycosylation and the trimeric conformation of the human immunodeficiency virus-1 (HIV-1) envelope protein limit the accessibility of some cellular glycan processing enzymes and end up with high-mannose-type

Increasing evidence has shown that circular RNAs (circRNAs) participate in the process of cardiac remodeling. CircRNA circ_0036176 originating from the back-splicing of exon 2 to exon4 of myosin IXA (Myo9a) gene was shown to be increased in the myocardium of patients with heart failure (HF) and riched in exosomes from human AC16 cardiomyocytes with overexpression of circ_0036176. Proliferation activity was inhibited in mCFs subjected to exosomal circ_0036176 treatment and in mCFs with overexpression of circ_0036176. Interestingly, circ_0036176 contains an IRES element and an ORF of 627 nt encoding a 208-amino acid protein (termed as Myo9a-208). Myo9a-208 was shown to mediate the inhibitory effect of circ_0036176 on CFs proliferation, and miR-218-5p could inhibit Myo9a-208 expression by binding to circ_0036176, resulting in abolishing the effect of circ_0036176 on inactivating cyclin/Rb signal and suppressing CFs proliferation. Our findings suggest that circ_0036176 inhibits mCFs proliferation by translating Myo9a-208 protein to suppress cyclin/Rb pathway.

Shell colors that exhibit a positive relationship with excellent traits can be employed as marker colors for breeding new varieties of bivalves. The clam Cyclina sinensis is an economically important marine bivalve that has three main colors: black, white, and purple. In the present study, we cloned and analyzed the full-length cDNA of the tyrosinase gene (TYR), which is the key gene for melanin synthesis, to explore the formation mechanism of black shells. The full-length cDNA of TYR was cloned by RACE-PCR, and the results showed that the full-length cDNA of TYR was 2993 bp, including a 2262 bp open reading frame encoding 753 amino acids. The results of functional domain prediction showed that deduced TYR protein contains a typical functional domain of tyrosinase, the common central domain. Phylogenetic analysis was conducted using neighbor-joining and maximum likelihood methods, and the results indicated that the evolutionary positions of C. sinensis and Meretrix meretrix were closest. The qRT‒PCR results showed that TYR mRNA was highly expressed in the outer edge of the mantle, which suggested that TYR was involved in the synthesis of melanin in the mantle of C. sinensis and might play an important regulatory role in the formation of melanin in black clam shells.

The information generated by the multifaceted and multilevel processing of the data of multiple sensors is more meaningful than the information obtained by a single sensor and provides accurate information and decision-making basis for various application systems. How to apply data mining theory to the multisensor cross-media field has become a research hotspot. Through the analysis of multisensor cross-media data, it is of great significance to dig out the important rules, information, or knowledge hidden in it and use it for cross-media retrieval engine. The rule acquisition is the “bottleneck” problem of the expert system. This paper adopts the data mining method based on the rough set to acquire the rules and improves the basic algorithm of attribute reduction. Using the attribute reduction algorithm and the heuristic value reduction algorithm, the calculation is simplified and the reduction efficiency is improved. In the presentation, according to the characteristics of cross-media and the application requirements of expert systems, this paper takes the case representation based on features as the basis and classifies cases according to feature attributes. In case retrieval, according to the hierarchical structure of case features, the entire case database is organized into a multilevel hierarchical index structure. In this paper, a cross-media retrieval engine is constructed from the perspective of classifier design, and the Euclidean distance is used as the similarity matching model of image content. The mutual retrieval of images and audios preliminarily forms the design process of retrieval from one media type to another and establishes a corresponding cross-media index. The experimental results show that the algorithm has better processing effect and higher accuracy than other algorithms. Different k-nearest neighbor values were selected in the experiment, and it reached about 96% in the test environment of libsvm toolbox, which is better than the processing results of LE and LLE algorithms.

High-density storage and neuromorphic devices based on 2D materials are hindered by large-scale growth. Moreover, the lack of a mature mechanism makes it difficult to obtain high-quality single crystals in large-scale 2D materials. In this work, we prepared a centimeter-scale single crystal α-MoO

Chemiluminescence (CL), as one class of luminescence driven by chemical reaction, exhibits obvious temperature-dependence in its light emission process. Herein, temperature-dependent CL emission of carbon nanodots (CDs) in the chemical reaction of peroxalate and hydrogen peroxide is demonstrated and temperature imaging based on the temperature-dependent CL has been established for the first time. In detail, the temperature-dependent CL emission of CDs in the chemical reaction of peroxalate and hydrogen peroxide is observed, and the linear relationship between the CL intensity and temperature is demonstrated in both the CL solution and film, enabling their applications in temperature sensing and imaging capabilities. The increase of the CL emission with temperature can be attributed to the accelerated electron exchange between the CDs and intermediate generated in the peroxalate system. Meter-scale chemiluminescent CD films have been constructed. The CL sensor based on the films presents a high spatial resolution of 0.4 mm and an outstanding sensitivity of 0.08 °C

A high-quality 2-inch polycrystalline diamond was prepared by MPCVD technique. Photodetector planar arrays were then fabricated from the polycrystalline diamond. Clear solar-blind images have been obtained using the arrays as the sensing unit.

Three new phenylpropenoid glycosides (1–3), and five undescribed shikimic acid derivatives (4–8), together with six known analogues (9–14), were obtained from the dried leaves of Illicium dunnianum.