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Heterojunction-driven photocatalysis can degrade various organic pollutants, and developing carbon nitride-based composite photocatalysts is of great significance and gains growing interest. In this study, a two-dimensional graphitic carbon nitride nanosheets/BiFeO3 (GCNNs/BiFeO3) Z-scheme heterojunction has been synthesized through the electrostatic spinning and post-calcination The obtained GCNNs/BiFeO3 nanofibers show large surface contact between GCNNs the and BiFeO3 nanostructures. The Z-scheme heterojunction shows a remarkably enhanced photocatalytic performance, which could degrade 94% of tetracycline (TC) and 88% of Rhodamine B (RhB) under LED visible light irradiation in 150 min. Radical trapping experiments demonstrate the effective construction of Z-scheme heterojunctions, and •O2- and h+ are the main active species in the photocatalytic degradation process. This study realizes a novel nanostructured GCNNs/BiFeO3 heterojunction for photodegradation applications, which would guide the design of next-generation efficient photocatalysts.

High-silica phosphogypsum (PG) is a kind of industrial by-product with great utilization potential. However, it is difficult to reuse PG directly due to the related gangue minerals (e.g., SiO2), and thus efficient purification is required to allow its further applications. Herein, a typical high-silica phosphogypsum waste was purified by a new "reverse-direct flotation" method. The organic matters and fine slimes were removed by reverse flotation, and then, the silica impurity was removed by direct flotation. Via the closed-circuit flotation process, the whiteness of the PG concentrate is improved from 33.23 to 63.42, and the purity of gypsum in the PG concentrate increases from 83.90% to 96.70%, with a gypsum recovery of 85%. Additionally, the content of SiO2 is significantly reduced from 11.11% to 0.07%. In-depth investigations suggest that the difference in the floatability of gypsum and quartz is prominently intensified by flotation reagents at pH = 2-2.5, and thus leads to good desilication performance. Further characteristics of the PG concentrate prove that impurities have been well removed, and the PG concentrate meets the requirement of related standards for gypsum building materials. The flotation method reported here paves the way for the purification of high-silica phosphogypsum, which can be extended to the purification and value-added reutilization of other industrial solid wastes.

The photoaging mechanisms of various polymers have been explored based on the basic autoxidation scheme (BAS) before 10 years ago, however current research verified some defects in the BAS in both thermodynamic and dynamics. These defects are troublesome because they are associated with the hydrogen abstraction which is central to continuously perform the photooxidation process of microplastics. These found indicated that we might wrongly inferred photo-oxidation process of some microplastics. In addition, the important role of reactive oxygen species (ROS) in the type-dependent photoaging process of various microplastics has been revealed recently. In this case, fully and accurately understanding the photoaging mechanisms of different microplastics in environment is a priority to further manage the ecological risk of microplastics. Herein, this review aims to revise and update the degradation process of microplastics based on the revised BAS and in the perspective of ROS. Specifically, the modification of BAS is firstly discussed. The photoaging mechanisms of representative microplastics (i.e., polyethylene, polystyrene and polyethylene terephthalate) are then updated based on the corrected BAS. Additionally, the role of ROS in their photolysis process and the possibility of microplastics as photosensitizers/mediators to regulate the fate of co-existent pollutants are also analyzed. Finally, several perspectives are then proposed to guide future research on the photoaging behaviors of microplastics. This review would pave the way for the understanding of microplastic photoaging and the management of plastic pollution in environments.

Bioenergy recovery in the form of medium-chain fatty acids (MCFAs) from waste activated sludge (WAS) is increasingly attractive, which are valuable building blocks for fuel production. This study experimentally demonstrated the long-term MCFAs (C6-C8) production from WAS in two-stage anaerobic sludge fermentation at different pH conditions, using continuously operated bench-scale anaerobic reactors. The WAS was continuously converted to short chain fatty acids (SCFAs, 3500-3800 mg chemical oxygen demand (COD)/L) at the first stage via alkaline anaerobic fermentation, which was directly fed into the second stage as both substrates and inoculum for MCFAs production through chain elongation (CE). The productions of MCFAs at the second stage were continuously studied under three different pH conditions (i.e., 10, 7 and 5.5). The results demonstrated that there was no significant MCFAs production at pH 10 during the steady state, whereas the MCFAs productions were clearly observed at both pH 7 and pH 5.5, with much higher MCFAs production from WAS at pH 7 (i.e., 10.32 g COD/L MCFAs) than that at pH 5.5 (i.e., 8.73 g COD/L MCFAs) during the steady state. A higher MCFAs selectivity of 62.3% was also achieved at pH 7. The relatively lower MCFAs production and selectivity at pH 5.5 was likely due to the higher undissociated MCFAs generated at pH 5.5, which would pose toxicity impact on CE microbes and thus inhibit the CE process. Microbial community analysis confirmed that the relative abundances of CE related microbes (e.g., Clostridium sensu stricto 12 sp. and Clostridium sensu stricto 1) increased at pH 7 compared to those at pH 5.5, which enabled more efficient MCFAs production from WAS.

To reclaim nitrous oxide (N2O) as an energy resource economically, this study developed an autotrophic denitrification-based system with thiosulfate (S2O32-) and nitric oxide (NO) as electron donor and acceptor, respectively. NO from flue gases is absorbed on Fe(II)EDTA to overcome its low solubility in liquid phase by forming Fe(II)EDTA-NO. Short-term batch tests and long-term continuous experiments were conducted to investigate the N2O production profile and NO conversion efficiency from thiosulfate-based denitrification under varied Fe (II)EDTA-NO conditions (5-20 mM). Up to 39% of NO was converted to gaseous N2O at 20 mM Fe(II)EDTA-NO amid batch test due to the inhibition of key enzymatic activities by NO and the acidic conditions following thiosulfate oxidation. Higher Fe(II)EDTA-NO levels induced lower enzymatic activities with N2OR being suppressed harder than NOR. Microbial diversity was reduced in the continuous thiosulfate-driven Fe(II)EDTA-NO-based denitrification system. NO-resistant bacteria and sulfide-tolerant denitrifiers were enriched, facilitating NO conversion to N2O thereafter.

This work aimed to reflect the advancements in water-related science, technology, and policy and shed light on future research opportunities related to water through a systematic overview of Water Research articles published in the first 21.5 years of the 21st century. Specific bibliometric analyses were performed to i) reveal the temporal and spatial trends of water-related research themes and ii) identify the underlying connections between research topics. The results showed that while top topics including wastewater (treatment), drinking water, adsorption, model, biofilm, and bioremediation remained constantly researched, there were clear shifts in topics over the years, leading to the identification of trending-up and emerging research topics. Compared to the first decade of the 21st century, the second decade not only experienced significant uptrends of disinfection by-products, anaerobic digestion, membrane bioreactor, advanced oxidation processes, and pharmaceuticals but also witnessed the emerging popularity of PFAS, anammox, micropollutants, emerging contaminants, desalination, waste activated sludge, microbial community, forward osmosis, antibiotic resistance genes, resource recovery, and transformation products. On top of the temporal evolution, distinct spatial evolution existed in water-related research topics. Microplastics and Covid-19 causing global concerns were hot topics detected, while metagenomics and machine learning were two technical approaches emerging in recent years. These consistently popular, trending-up and emerging research topics would most likely attract continuous/increasing research input and therefore constitute a major part of the prospective water-related research publications.

The coupling of photocatalytic and algal processes has been used for the removal of widespread antibiotics. The removal capacities of the individual and the combined system against enrofloxacin were tested and compared in this work. Due to the low tolerance of the algae to enrofloxacin, the target compound was barely degraded during the individual algal treatment. In the individual photocatalytic process, the mineralization efficiency (defined as the ratio between the produced carbon dioxide and the initial) reached ∼57% with the remaining formed as transformation products. In contrast, a two-stage treatment incorporating photocatalytic and algal processes removed enrofloxacin completely and increased the mineralization efficiency to ∼64% or more. The addition of the citric acid as external co-substrate further elevated the mineralization efficiency with a factor of 1.25 compared to that of the individual photocatalysis. Different degradation products in both individual and integrated processes were identified and compared. The degradation pathways were found to involve the attack of the piperazine moiety and quinolone core. The results indicated the potential application of the combined photocatalytic-algal treatment in removal of veterinary antibiotics and improved our understanding of the underlying mechanisms and pathways.

Food waste (FW) is not only a major social, nutritional and environmental issue, but also an underutilized resource with significant energy, which has not been fully explored currently. Considering co-digestion can adjust carbon to nitrogen ratio (C/N) of the feedstock and improve the synergetic interactions among microorganisms, anaerobic co-digestion (AnCoD) is then becoming an emerging approach to achieve higher energy recovery from FW while ensuring the stability of the system. To obtain higher economic gain from such biodegradable wastes, increasing attention has been paid on optimizing the system configuration or applying enzymatic hydrolysis before digesting FW. A better understanding on the potentiality of correlating enzymatic pretreatment and AnCoD operated in various system configuration would enhance the bioresource recovery from FW and increase revenue through treating this organic waste. Specifically, the biobased chemicals outputs from FW-related co-digestion system with different configuration were firstly compared in this review. A deep discussion concerning the challenges for achieving bioresources recovery from FW co-digestion systems with enzymatic pretreatment was then given. Recommendations for future studies regarding FW co-digestion were then proposed at last.

Previous studies mostly focused on the responses of anaerobic granular sludge (AGS) to one kind of microplastics during wastewater treatment. However, a wide variety of microplastics has been detected in wastewater. The multiple microplastics induced stress on AGS and the effectively mitigating strategy still remain unavailable. Herein, this work comprehensively excavated the influences of multiple microplastics (i.e., polyethylene terephthalate (PET), polystyrene (PS), polyethylene (PE) and polypropylene (PP)) coexisting in the wastewater on AGS system from macroscopic to microcosmic aspects. Experimental results illustrated that microplastics decreased AGS granule size, increased cell inactivation and caused deteriorative methane recovery from wastewater. As such, this study then put great emphasis on proposing a mitigating strategy using hydrochar and disclosing the role of hydrochar in overcoming the stress induced by coexisting microplastics to AGS system. Physiological characterization and microbial community analysis demonstrated that hydrochar effectively mitigated the reductions in methane production by 50.6% and cell viability by 68.8% of microplastics-bearing AGS and reduced the toxicity of microplastics to microbial community in the AGS. Mechanisms investigation by fluorescence tagging and excitation emission matrix fluorescence spectroscopy with fluorescence regional integration (EEM-FRI) analysis revealed that hydrochar adsorbed/accumulated microplastics and enhanced microplastics-bearing AGS to secrete extracellular polymeric substance (EPS) with more humic acid generation, thus reducing the direct contact between microplastics and AGS. In addition, hydrochar weakened the AGS intracellular oxidative stress induced by microplastics, thereby completely eliminating the inhibition of microplastics on acidification efficiency of AGS, and partially mitigating the suppression on methanation.

The enrichment of polyhydroxyalkanoates (PHA) accumulating bacteria (PAB) in mixed microbial cultures (MMC) is extremely difficult to be predicted and optimized. Here we demonstrate that mechanistic and deep learning models can be integrated innovatively to accurately predict the dynamic enrichment of PAB. Well-calibrated activated sludge models (ASM) of the PAB enrichment process provide time-dependent data under different operating conditions. Recurrent neural network (RNN) models are trained and tested based on the time-dependent dataset generated by ASM. The accurate prediction performance is achieved (R2 > 0.991) for three different PAB enrichment datasets by the optimized RNN model. The optimized RNN model can also predict the equilibrium concentration of PAB (R2 = 0.944) and corresponding time, which represents the end of the PAB enrichment process. This study demonstrates the strength of integrating mechanistic and deep learning models to predict long-term variations of specific microbes, helping to optimize their selection process for PHA production.

The ubiquitous micro/nanoplastics (MPs/NPs) in urban waters are priority pollutants due to their toxic effects on living organisms. Currently, great efforts have been made to realize a plastic-free urban water system, and the identification and removal of MPs/NPs are two primary issues. Among diverse methods, emerging electrochemical techniques have gained growing interests owing to their facile implementation, high efficiency, eco-compatibility, onsite operation, etc. Herein, recent progress in the electrochemical identification and removal of MPs/NPs in urban waters are comprehensively reviewed. The electrochemical sensing of MPs/NPs and their released pollutants (e.g., bisphenol A (BPA)) has been analyzed, and the sensing principles and the featured electrochemical devices/electrodes are examined. Afterwards, recent applications of electrochemical methods (i.e., electrocoagulation, electroadsorption, electrokinetic separation and electrochemical degradation) in MPs/NPs removal are discussed in detail. The influences of critical parameters (e.g., plastics' property, current density and electrolyte) in the electrochemical identification and removal of MPs/NPs are also analyzed. Finally, the current challenges and prospects in electrochemical sensing and removal of MPs/NPs in urban waters are elaborated. This review would advance efficient electrochemical technologies for future MPs/NPs pollutions management in urban waters.

Tightly bound extracellular polymeric substances (TB-EPS) and loosely bound extracellular polymeric substances (LB-EPS) affect the flocculability and settleability of sludge and the transfer of oxygen, which are highly related to aeration. In this study, we systemically evaluated the expanded unified model-TL2.1 for its long-term simulation of TB-EPS and LB-EPS. Two different aeration conditions and three different influent carbon sources were used to evaluate the model, and the simulation results fit well with the experimental data. TB-EPS and LB-EPS production increased with aeration intensity. The influence of aeration parameters on TB-EPS and LB-EPS production in a short-term batch system and long-term sequencing batch reactor (SBR) system was compared. The aeration parameters included the total transfer coefficient (kLa) and the concentration of dissolved oxygen at the interface (CS). To ensure a high removal rate of substrates and ammonia nitrogen and achieve a stable active biomass concentration, the following aeration parameters can be adopted to reduce energy wastage during aeration: when CS is 2 mg/L, kLa can be set above 30 h-1 and below 50 h-1; when kLa is 50 h-1, CS can be set above 1 mg/L and below 1.5 mg/L. This study systematically revealed the influence of aeration on TB-EPS and LB-EPS formation in an SBR system through a mathematical model, and it provides a theoretical basis for better understanding aeration.

Primary sludge (PS) and Waste activated sludge (WAS) as two main sludge streams in wastewater treatment plants are commonly anaerobically co-digested, which though may be differently affected by pretreatment. Previous work has found that calcium peroxide (CaO2) pretreatment effectively enhanced anaerobic digestion of WAS. However, the feasibilities of this strategy on PS anaerobic digestion and co-digestion of WAS and PS are still unclear. Herein, this work provided new insights into these systems. Biomethane potential test demonstrated that CaO2 pretreatment at 0.02-0.26 g/g-volatile suspended solids (VSS) promoted anaerobic digestion of PS. Then the feasibility of CaO2 pretreatment for improving anaerobic co-digestion of PS and WAS mixture was confirmed, with the highest improvement in methane production, VSS destruction and sludge reduction being approximately 37.4%, 38.9% and 19.9%, achieved at 0.14 g/g-VSS of CaO2. Process modelling analysis revealed that CaO2 pretreatment increased both degradable faction and actually degraded fraction in sludge mixture. The changes of sludge characteristics via pretreatment and key enzyme activity in sludge anaerobic co-digestion system demonstrated that increased CaO2 concentration resulted in increased soluble organics release from sludge mixture in the pretreatment stage and inhibited activity of coenzyme F420 responsible for methanogenesis. Further mechanism investigation disclosed that OH-, O2- and OH were main contribution factors, and the order of their contributions were OH- >O2- >OH. This work laid the theoretical foundation and provided guidance for the practical application of CaO2 pre-treatment technology.

Integrating anammox with denitrifying anaerobic methane oxidation (DAMO) in the membrane biofilm reactor (MBfR) is a promising technology capable of achieving complete nitrogen removal from wastewater. However, it remains unknown whether reactor configurations featuring longitudinal gradients parallel to the membrane surface would affect the performance of the CH4-driven MBfR. To this end, this work aims to study the impacts of longitudinal heterogeneity potentially present in the gas and liquid phases on a representative CH4-driven MBfR performing anammox/DAMO by applying the reported modified compartmental modeling approach. Through comparing the modeling results of different reactor configurations, this work not only offered important guidance for better design, operation and monitoring of the CH4-driven MBfR, but also revealed important implications for prospective related modeling research. The total nitrogen removal efficiency of the MBfR at non-excessive CH4 supply (e.g., surface loading of ≤0.064 g-COD m-2 d-1 in this work) was found to be insensitive to both longitudinal gradients in the liquid and gas phases. Comparatively, the longitudinal gradient in the liquid phase led to distinct longitudinal biomass stratification and therefore played an influential role in the effective CH4 utilization efficiency, which was also related to the extent of reactor compartmentation considered in modeling. When supplied with non-excessive CH4, the MBfR is recommended to be designed/operated with both the biofilm reactor and the membrane lumen as plug flow reactors (PFRs) with co-current flow of wastewater and CH4, which could mitigate dissolved CH4 discharge in the effluent. For the reactor configurations with the biofilm reactor designed/operated as a PFR, multi-spot sampling in the longitudinal direction is needed to obtain a correct representation of the microbial composition of the MBfR.

With the increasing demand for sustainable development, the recycling and utilization of wastes has received widespread attention. This study proposed a green method of using one waste, corncob ash, to boost microbial the production of hydrogen from another waste, waste activated sludge, during anaerobic fermentation. The corncob ash dosage and the fermentative hydrogen production was positively correlated, and the maximum production of hydrogen reached up to 46.8 ± 1.0 mL/g VS, which was about 3.5 times that of the control group without corncob ash dosage (17.0 ± 0.9 mL/g VS). Mechanistic studies found that corncob ash was beneficial to the solubilization, hydrolysis and acetogenesis processes involved in fermentative hydrogen production process. The microbial community analysis indicated that corncob ash enriched more hydrolytic microorganisms (e.g., Bacteroides sp. and Leptolinea sp.), and has less impact on acidifying microorganisms, compared to the control group. The strategy of using corncob ash to boost the production of hydrogen during anaerobic waste activated sludge fermentation proposed in this study might provide a new waste-control-waste paradigm, making sludge disposal and wastewater treatment more sustainable.

Microplastics (MPs) have been frequently detected in effluent wastewater and sludge in wastewater treatment plants (WWTPs), the discharge and agricultural application of which represent a primary source of environmental MPs contamination. As important as quantitative removal is, changes of physicochemical characteristics of MPs (e.g., shapes, sizes, density, crystallinity) in WWTPs are crucial to their environmental behaviors and risks and have not been put enough attention yet. This review is therefore to provide a current overview on the changes of physicochemical characteristics of MPs in WWTPs and their corresponding environmental risks. The changes of physicochemical characteristics as well as the underlying mechanisms of MPs in different successional wastewater and sludge treatment stages that mainly driven by mechanical (e.g., mixing, pumping, filtering), chemical (e.g., flocculation, advanced oxidation, ultraviolet radiation, thermal hydrolysis, incineration and lime stabilization), biological (e.g., activated sludge process, anaerobic digestion, composition) and their combination effects were first recapitulated. Then, the inevitable correlations between physicochemical characteristics of MPs and their environmental behaviors (e.g., migration, adsorption) and risks (e.g., animals, plants, microbes), are comprehensively discussed with particular emphasis on the leaching of additives and physicochemical characteristics that affect the co-exist pollutants behavior of MPs in WWTPs on environmental risks. Finally, knowing the summarized above, some relating unanswered questions and concerns that need to be unveiled in the future are prospected. The physicochemical properties of MPs change after passing through WWTP, leading to subsequent changes in co-contaminant adsorption, migration, and toxicity. This could threaten our ecosystems and human health and must be worth investigating.

The traditional synthesis of ammonia is an industrial process with high energy consumption that is not environmentally friendly; thus, it is urgent to develop cost-effective approaches to synthesize ammonia under ambient conditions. In recent years, the photochemical synthesis of ammonia has become a hot research frontier. In this mini review, we summarize the recent advances in materials sciences for photocatalytic nitrogen fixation. Beyond nitrogen fixation, we talk about an alternative for artificial ammonia synthesis and coupling reactions with other reactions for the synthesis of other high-value chemicals. The results and findings of this review will help the development of ammonia synthesis and the synthesis of other high-value chemicals.

Sewage sludge (SS) is increasingly used as an environment functional material to reduce or control pollution and improve plant growth because of the large amounts of carbon and essential plant nutrients in it. To achieve the best application results, it is essential to comprehensively review recent progress in SS utilization. This review aims to fill the gaps in knowledge by describing the properties of SS, and its usage as adsorbents, catalysts and fertilizers, and certain application mechanisms. Although SS generates several benefits for the environment and humans, many challenges still exist to limit the application, including the risks posed by potentially toxic substances (e.g., heavy metals) in SS. Therefore, future research directions are discussed and how to make SS applications more feasible in terms of technology and economy.

Mathematical modeling plays a critical role toward the mitigation of nitrous oxide (N2O) emissions from wastewater treatment plants (WWTPs). In this work, we proposed a novel hybrid modeling approach by integrating the first principal model with deep learning techniques to predict N2O emissions. The hybrid model was successfully implemented and validated with the N2O emission data from a full-scale WWTP. This hybrid model is demonstrated to have higher accuracy for N2O emission modeling in the WWTP than the mechanistic model or pure deep learning model. Equally important, the hybrid model is more applicable than the pure deep learning model due to the lower requirement of data and the pure mechanistic model due to the less calibration requirement. This superior performance was due to the hybrid nature of the proposed model. It integrated the essential wastewater treatment knowledge as the first principal component and the less understood N2O production processes by the data-driven deep learning approach. The developed hybrid model was also successfully implemented under different circumstances for the prediction of N2O flux, which showed the generalizability of the model. The hybrid model also showed great potential to be applied for the N2O mitigation work. Nevertheless, the capability of the hybrid model in evaluating N2O mitigation strategies still requires validation with experiments. Going beyond N2O modeling in WWTP, the novel hybridization modeling concept can potentially be applied to other environmental systems.

This work put up a novel strategy of applying calcium peroxide (CaO2) in aerobic sludge digestion and provided insights into such system. The degradation percentage of sludge and total inorganic nitrogen production in the digesters with CaO2 at 0.02 g/g-VS-WAS increased by 25.8% and 18.8% of control. CaO2 addition allowed various key microbes related to organics degradation to accumulate in the system. Moreover, the modelling and chemical (i.e., excitation emission matrix (EEM) fluorescence and fourier transformation spectroscopy (FTIR)) analyses revealed that CaO2 addition enhanced sludge biodegradability with more release of biodegradable organics and increased degradation of recalcitrant organics, which can be transformed into biodegradable organics with the action of CaO2. Subsequent transformation test indicated that CaO2 enabled to promote hydrolysis and catabolism of biodegradable substrates in sludge. Further investigations on function mechanism suggested that CaO2 carried on positive action for sludge aerobic digestion mainly through the enhancement by ·OH.

Anaerobic digestion (AD) is a low-cost technology widely used to divert waste activated sludge (WAS) to renewable energy production, but is generally restricted by its poor biodegradability which mainly caused by the endogenous and exogenous refractory substances present in WAS. Several conventional methods such as thermal-, chemical-, and mechanical-based pretreatment have been demonstrated to be effective on organics release, but their functions on refractory substances conversion are overlooked. This paper firstly reviewed the presence and role of endogenous and exogenous refractory substances in anaerobic biodegradability of WAS, especially on their inhibition mechanisms. Then, the pretreatment strategies developed for enhancing WAS biodegradability by facilitating refractory substances conversion were comprehensively reviewed, with the conversion pathways and underlying mechanisms being emphasized. Finally, the future research needs were directed, which are supposed to improve the circular bioeconomy of WAS management from the point of removing the hindering barrier of refractory substances on WAS biodegradability.

The global spread of antibiotic resistance genes (ARGs) has wreaked havoc with the treatment efficiency of antibiotics and, ultimately, anti-microbial chemotherapy, and has been conventionally attributed to the abuse and misuse of antibiotics. However, the ancient ARGs have alterative functions in bacterial physiology and thus they could be co-regulated by non-antibiotic conditions. Recent research has demonstrated that many non-antibiotic chemicals such as microplastics, metallic nanoparticles and non-antibiotic drugs, as well as some non-antibiotic conditions, can accelerate the dissemination of ARGs. These results suggested that the role of antibiotics might have been previously overestimated whereas the effects of non-antibiotic conditions were possibly ignored. Thus, in an attempt to fully understand the fate and behavior of ARGs in the eco-system, it is urgent to critically highlight the role and mechanisms of non-antibiotic chemicals and related environmental factors in the spread of ARGs. To this end, this timely review assessed the evolution of ARGs, especially its function alteration, summarized the non-antibiotic chemicals promoting the spread of ARGs, evaluated the non-antibiotic conditions related to ARG dissemination and analyzed the molecular mechanisms related to spread of ARGs induced by the non-antibiotic factors. Finally, this review then provided several critical perspectives for future research.

This work studied the impacts of key granule properties on the granular reactor performing partial nitritation/anammox from the modeling perspective. The results could guide not only future reliable modeling but also practical startup/operation of the reactor. To achieve high total nitrogen (TN) removal whilst avoiding significant N2O production, inoculated granules should be big and anammox-enriched. The optimum boundary layer thickness for maximum TN removal increased with the decreasing diffusivity of soluble components in the granule structure. Even though a thick boundary layer could protect anammox bacteria from elevated dissolved oxygen (DO) (e.g., 0.5 g-O2/m3) and obtain high TN removal (>90.0%) and low N2O production (<1.8%), even complete removal of the boundary layer would fail to provide sufficient substrate for anammox and therefore couldn't increase TN removal to 90.0% and decrease N2O production to <2.4% at insufficient DO (e.g., 0.3 g-O2/m3 in the presence of lifted influent NH4+ concentration).

The omnipresent micro/nanoplastics (MPs/NPs) in urban waters arouse great public concern. To build a MP/NP-free urban water system, enormous efforts have been made to meet this goal via separating and degrading MPs/NPs in urban waters. Herein, we comprehensively review the recent developments in the separation and degradation of MPs/NPs in urban waters. Efficient MP/NP separation techniques, such as adsorption, coagulation/flocculation, flotation, filtration, and magnetic separation are first summarized. The influence of functional materials/reagents, properties of MPs/NPs, and aquatic chemistry on the separation efficiency is analyzed. Then, MP/NP degradation methods, including electrochemical degradation, advanced oxidation processes (AOPs), photodegradation, photocatalytic degradation, and biological degradation are detailed. Also, the effects of critical functional materials/organisms and operational parameters on degradation performance are discussed. At last, the current challenges and prospects in the separation, degradation, and further upcycling of MPs/NPs in urban waters are outlined. This review will potentially guide the development of next-generation technologies for MP/NP pollution control in urban waters.

Water is crucial for economic development since it interacts with the agricultural, production, and energy sectors. However, the increasing demand and climate change put pressure on water sources. This paper argued the necessity of using reclaimed water for irrigation within the scope of a circular economy. The barriers (i.e., technological and economic, institutional/regulatory, and social) to water reuse practices were revealed. Lessons on how to overcome the barriers were learned from good practices. The roadmaps adopted in the European Union for the transition towards the circular economy were reviewed. It has been observed that these roadmaps are generally on the circularity of solid wastes. However, water is too important for the economy to be ignored in the transition towards circular economy. Research needs and perspective for a comprehensive roadmap to widen water-smart solutions such as water reuse were drawn.

This study describes an integrated granular sludge and fixed-biofilm (iGB) reactor innovatively designed to carry out the anammox/partial-denitrification (A/PD) process for nitrogen removal with mainstream municipal wastewater. The iGB-A/PD reactor consists of anammox granules inoculated in the lower region of reactor and an acclimated fixed-biofilm positioned in the upper region. Compared to the other reported A/PD systems for mainstream wastewater treatment, this iGB-A/PD reactor is notable due to its higher quality effluent with a total inorganic nitrogen (TIN) of ∼3 mg•L-1 and operation at a high nitrogen removal rate (NRR) of 0.8 ± 0.1 kg-N•m-3•d-1. Reads-based metatranscriptomic analysis found that the expression values of hzsA and hdh, key genes associated with anammox, were much higher than other functional genes on nitrogen conversion, confirming the major roles of the anammox bacteria in nitrogen bio-removal. In both regions of the reactor, the nitrate reduction genes (napA/narG) had expression values of 56-99 RPM, which were similar to that of the nitrite reduction genes (nirS/nirK). The expression reads from genes for dissimilatory nitrate reduction to ammonium (DNRA), nrfA and nirB, were unexpectedly high, and were over the half of the levels of reads from genes required for nitrate reduction. Kinetic assays confirmed that the granules had an anammox activity of 16.2 g-NH4+-N•kg-1-VSS•d-1 and a nitrate reduction activity of 4.1 g-N•kg-1-VSS•d-1. While these values were changed to be 4.9 g- NH4+-N•kg-1-VSS•d-1and 4.3 g-N•kg-1-VSS•d-1 respectively in the fixed-biofilm. Mass flux determination found that PD and DNRA was responsible for ∼50% and ∼25% of nitrate reduction, respectively, in the whole reactor, consistent with high effluent quality and treatment efficiency via a nitrite loop. Metagenomic binning analysis revealed that new and unidentified anammox species, affiliated with Candidatus Brocadia, were the dominant anammox organisms. Myxococcota and

Aerobic digestion is considered to be a common process for the stabilization of waste activated sludge (WAS) in the small-sized wastewater treatment systems, while the broad application of titanium dioxide nanoparticles (TiO2 NPs) results in their unavoidable existence in WAS aerobic digestion, with its role in aerobic sludge digestion being never documented. This study set up a series of aerobic sludge digesters to evaluate the previously unknown role of TiO2 NPs on the performance of the digesters. The volatile solids (VS) degradation percentage increased from 21.9 ± 0.6% to 26.9 ± 0.1% - 30.0 ± 0.3% with the different contents of TiO2 NPs (0, 1, 20 and 50 mg/L). Similarly, the total inorganic nitrogen production increased from 23.1 ± 0.3 to 31.0 ± 0.1 mg N/g VS with the rising TiO2 NPs concentrations from 0 to 50 mg/L. The microbial analysis suggested that TiO2 NPs contributed to the accumulation of specific microbes correlated with the degradation of organic substances and the conversion of nitrogen compounds. Model-based analysis showed the higher biodegradability and hydrolysis rate of sludge with TiO2 NPs. Further mechanistic studies indicated that the enhancement of WAS solubilization and the degradation of recalcitrant substances (e.g., humic acid and cellulose) contributed to the better performance of experimental aerobic digesters, which was confirmed by the fourier transformation infrared spectroscopy (FTIR) indicating the converting of these materials into biodegradable substrates for digestion with TiO2 NPs. It could be inferred from this investigation that aerobic digestion rather than anaerobic digestion would be a more suitable treatment method for sludge containing TiO2 NPs.

Microplastic fibers (MFs), as the most frequently detected microplastic shape in sewage sludge, have posed emerging concern for sludge treatment and disposal. However, the effect of MFs on antibiotic resistance genes (ARGs), especially extracellular ARGs (eARGs) during sludge treatment remains far from explicit. Therefore, this study investigated the potential impact of MFs on eARGs during sludge anaerobic digestion (AD), a commonly used sludge treatment method, through long-term operation. The qPCR results showed that both absolute and relative abundances of eARGs increased with the MFs exposure during sludge AD. The average absolute and relative abundances of eight tested eARGs in the AD reactor with the highest MFs dosage (170 items/gTS) were 1.70 and 2.15 times higher than those in the control AD reactor. The metagenomics results further comfirmed the increase of eARGs abundance during sludge anaerobic digestion after MFs exposure and the enhancement did not show significant selectivity. The identification of the potential hosts of eARGs suggested the host numbers of eARGs also increased with MFs exposure, which may suggest enhanced horizonal transformation as a result of increased eARGs. Further exploring the mechansims showed that the genes involved in type IV secretion system was upregulated after MFs exposure, suggesting the active release of eARGs was enhanced with MFs exposure. In contrast, the MFs may not affect the passive release of eARGs as its impact on cell membrance damage was insignificant. The enhanced eARGs in sludge AD process may further accelerate the transport of ARGs in environment, which should be considered during sludge treatment and disposal.

Metal-laden solid wastes (e.g., waste graphite powders) have attracted great attention owing to their hazardous effects on the surrounding soil and water. Additionally, the metal-bearing impurities also hinder the reutilization of waste graphite powders. Thus, it is necessary to remove these inorganic impurities and figure out the removal mechanism of impurities in the purification process. In this study, an alkaline roasting-water washing-acid leaching (AWA) method was used to upgrade the waste graphite powders, and the migration behavior of diverse impurities has been qualitatively and quantitatively investigated. A graphite product with high impurity removal efficiencies is attained under optimal conditions. The removal of impurities mainly follows three routes: (1) V-, P-, and S-bearing impurities were complete removed (some formed soluble salts during alkaline roasting, and the remainder was dissolved in acid); (2) most Al-, K-, and Si-bearing impurities were removed by alkaline roasting, with the remainder was dissolved in the acid-leaching process; and (3) Fe-, Mg-, Ti-, Ca-, and Zn-bearing impurities were decomposed at high temperature and reacted with alkali to form hydroxides or oxides, which was subsequently dissolved in acid. In addition, the treatment of the generated wastewater is also discussed. The uncovered migration mechanisms of diverse impurities would guide the purification and reutilization process of other metal-bearing solid wastes efficiently.

Microplastics and nanoplastics generally accumulated in waste activated sludge (WAS) after biological wastewater treatment. Currently, researches mainly focused on how plastics affected a particular sludge treatment method, without the comparison of different sludge systems. Herein, distinct responses of hydrogen-producing and methane-producing sludge systems were comprehensively evaluated with polystyrene microplastics (PS-MPs) and nanoplastics (PS-NPs) existence. Experimental results showed that PS particles would stimulate inhibition on anaerobic gas production except that PS-MPs were conducive to hydrogen accumulation, which was caused by the enhanced solubilization. Mechanistic investigation demonstrated that severe inhibition of PS-NPs to hydrogen production was derived from the excessively inhibitory hydrolysis despite of improving solubilization. Varying degrees of inhibition to acidification and methanation collectively contributed to reduced methane accumulation with exposure to PS-MPs and PS-NPs. Excessive oxidative stress would be generated in the presence of PS-MPs or PS-NPs, deteriorating microbial activities and richness of species responsible for hydrogen or methane production.

Anaerobic digestion (AD) is a promising method for treating waste activated sludge (WAS), but the low methane yield limits its large-scale application. The addition of conductive nanomaterials has been demonstrated to enhance the activity of AD via promoting the direct interspecies electron transfer (DIET). In this study, novel conductive polypyrrole (Ppy) was prepared to effectively improve the AD performance of WAS. The results showed that the accumulative methane production was enhanced by 27.83% by Ppy, with both acidogenesis and methanogenesis being efficiently accelerated. The microbial community analysis indicated that the abundance of bacteria associated with acidogenesis process was significantly elevated by Ppy. Further investigation by metatranscriptomics revealed that fadE and fadN genes (to express the key enzymes in fatty acid metabolism) were highly expressed in the Ppy-driven AD, suggesting that Ppy promoted electron generation during acid production. For methanogenesis metabolism, genes related to acetate utilization and CO2 utilization methanogenesis were also up-regulated by Ppy, illustrating that Ppy facilitates the utilization of acetate and electrons by methanogenic archaea, thus potentially promoting the methanogenesis through DIET.

While sulfur-driven autotrophic denitrification (SDAD) occurring in the anoxic reactor of the sulfate reduction, autotrophic denitrification and nitrification integrated (SANI) system has been regarded as the main nitrogen removal bioprocess, little is known about the accompanying Anammox bacteria whose presence is made possible by the co-existence of NH4+ and NO2-. Therefore, this work firstly developed an integrated SDAD-Anammox model to describe the interactions between sulfur-oxidizing bacteria and Anammox bacteria. The model was subsequently used to explore the impacts of influent conditions on the reactor performance and microbial community structure of the anoxic reactor. The results revealed that at a relatively low ratio of <1.5 mg S/mg N, Anammox bacteria could survive and even take a dominant position (up to 58.9%). Finally, a modified SANI system configuration based on the effective collaboration between SDAD and Anammox processes was proposed to improve the efficiency of the treatment of sulfate-rich saline sewage.

BiOX (X = Cl, Br and I) and BiOX/TiO2 photocatalysts were prepared by a facile hydrothermal approach. The BiOX/TiO2 heterojunctions demonstrated significantly enhanced efficiency for photocatalytic decomposition of perfluorooctanoic acid (PFOA) compared with sole BiOX or TiO2. PFOA (10 mg L1) was completely degraded by BiOCl(Br)/TiO2 in 8 h. Moreover, BiOCl/TiO2 attained deep decomposition of PFOA with a high defluorination ratio of 82%. The p-n heterojunctions between BiOX and TiO2 were confirmed by a series of characterizations. The photo-induced holes would migrate from the valance band (VB) of TiO2 to BiOX, driven by the built-in electric field (BIEF) near the interfaces of p-n heterojunctions, the inner electric fields (IEF) in BiOX and the higher VB position of BiOX. The X-ray diffraction (XRD) and TEM characterizations indicated that TiO2 combined with BiOX along the [110] facet, which facilitated photo-induced electron transfer in the [001] direction, thus benefiting PFOA decomposition.

The sustainable production of green hydrogen via water electrolysis necessitates cost-effective electrocatalysts. By following the circular economy principle, the utilization of waste-derived catalysts significantly promotes the sustainable development of green hydrogen energy. Currently, diverse waste-derived catalysts have exhibited excellent catalytic performance toward hydrogen evolution reaction (HER), oxygen evolution reaction (OER), and overall water electrolysis (OWE). Herein, we systematically examine recent achievements in waste-derived electrocatalysts for water electrolysis. The general principles of water electrolysis and design principles of efficient electrocatalysts are discussed, followed by the illustration of current strategies for transforming wastes into electrocatalysts. Then, applications of waste-derived catalysts (i.e., carbon-based catalysts, transitional metal-based catalysts, and carbon-based heterostructure catalysts) in HER, OER, and OWE are reviewed successively. An emphasis is put on correlating the catalysts' structure-performance relationship. Also, challenges and research directions in this booming field are finally highlighted. This review would provide useful insights into the design, synthesis, and applications of waste-derived electrocatalysts, and thus accelerate the development of the circular economy-driven green hydrogen energy scheme.

Waste activated sludge (WAS) contains high concentrations of microplastics (MPs), which could serve as vectors of various organic pollutants and heavy metals, causing synergistic transportation and pollution. The application of combined hydrothermal pretreatment (HTP) and anaerobic digestion (AD) has raised growing concerns since the low-temperature hydrothermal treatment could enhance the biogas production of WAS. However, the changes in physicochemical properties, adsorption performances, and effects on AD of MPs by HTP have not been studied. The study used three typical MPs in WAS, and it was found that the HTP (170°C & 30min) increased MPs' specific surface area and carbonyl index (CI) while decreasing the relative crystallinity. The adsorption capacity to Cd increased through the carbonylation for polyethylene microplastic (PE-MP) and polystyrene microplastic (PS-MP) while decreasing by the dechlorination for polyvinyl chloride microplastic (PVC-MP). Meanwhile, increased hydrophilicity reduced the adsorption capacities of all three typical MPs for ofloxacin. The above results indicated that the HTP could be worth blocking the adsorption of polar MPs for polar pollutants. For the pristine MPs, only PVC-MP at the highest concentration (0.5 g kg-1 VS) significantly (p < 0.05) reduced methane production by 16.2 ± 3.3% of WAS without the HTP. However, the HTP resulted in significant (p < 0.05) inhibition of methane production of WAS at high concentrations of PE-MP and PVC-MP (e.g., 0.1 and 0.5 g kg-1 VS), which was due to the acceleration of the released toxic plastic additives (dibutyl phthalate, dimethyl phthalate, and bisphenol-A). Microbial analysis showed the abundances of vital anaerobes, such as acid-producing bacteria (Acetoanerrobium and Mesotoga), proteolytic bacteria (Proteiniborus), and methanogens (Methanosaeta) clearly decreased with the PE-MP and PVC-MP after the HTP, which might result in the decreased methane production. The study provided deep-insi

Three-dimensional biofilm electrode reactors (3D-BERs) are highly efficient in refractory wastewater treatment. In comparison to conventional bio-electrochemical systems, the filled particle electrodes act as both electrodes and microbial carriers in 3D-BERs. This article reviews the conception and basic mechanisms of 3D-BERs, as well as their current development. The advantages of 3D-BERs are illustrated with an emphasis on the synergy of electricity and microorganisms. Electrode materials utilized in 3D-BERs are systematically summarized, especially the critical particle electrodes. The configurations of 3D-BERs and their integration with wastewater treatment reactors are introduced. Operational parameters and the adaptation of 3D-BERs to varieties of wastewater are discussed. The prospects and challenges of 3D-BERs for wastewater treatment are then presented, and the future research directions are proposed. We believe that this timely review will help to attract more attentions on 3D-BERs investigation, thus promoting the potential application of 3D-BERs in wastewater treatment.