101. CD3+ and/or CD14+ depletion from cord blood mononuclear cells before ex vivo expansion culture improves total nucleated cell and CD34+ cell yields
- Author
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Nina Shah, Mark F. Munsell, William K. Decker, Richard E. Champlin, Junjun Lu, Ann M. Leen, David Steiner, Hong Yang, Simon N. Robinson, Simrit Parmar, Paul J. Simmons, Elizabeth J. Shpall, Dongxia Xing, and Catherine M. Bollard
- Subjects
Blood Platelets ,CD3 Complex ,Platelet Engraftment ,Neutrophils ,Cell Culture Techniques ,Lipopolysaccharide Receptors ,Antigens, CD34 ,Cell Separation ,Peripheral blood mononuclear cell ,Article ,Andrology ,Blood cell ,Medicine ,Antigen-presenting cell ,Cell Proliferation ,Transplantation ,business.industry ,Cell growth ,Hematology ,Fetal Blood ,Hematopoietic Stem Cells ,Culture Media ,Hematopoiesis ,Haematopoiesis ,medicine.anatomical_structure ,Cord blood ,Immunology ,Leukocytes, Mononuclear ,business ,Ex vivo - Abstract
Cord blood (CB) is used increasingly in transplant patients lacking sibling or unrelated donors. A major hurdle to the use of CB is low cell dose which is largely responsible for elevated risk of graft failure and significantly delayed neutrophil and platelet engraftment. Since a positive correlation has been demonstrated between the total nucleated cell (TNC) and CD34+ cell dose transplanted and time to neutrophil and platelet engraftment, strategies to increase these measures are under development. One strategy includes the ex vivo expansion of CB mononuclear cells (MNC) with mesenchymal stem cells (MSC) in a cytokine cocktail. We demonstrate that this strategy can be further improved if CD3+ and/or CD14+ cells are first depleted from the CB MNC prior to ex vivo expansion. Ready translation of this depletion strategy to improve ex vivo CB expansion in the clinic is feasible since clinical-grade devices and reagents are available. Ultimately the goal of improving TNC and CD34+ transplant doses is to further improve the rate of neutrophil and platelet engraftment in CB recipients.
- Published
- 2009