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Chronic kidney disease (CKD) is common in geriatric cats, and is characterised in the majority of cases by tubulointerstitial inflammation and fibrosis. Hyperphosphataemia is a frequent complication of CKD and is independently associated with severity of renal fibrosis and disease progression. Transforming growth factor-beta 1 (TGF-β1) signalling is thought to be a convergent pathway which mediates the progression of renal fibrosis in CKD. The aims of this study were to explore the interaction between increased extracellular phosphate and the TGF-β1 signalling pathway by investigating: (a) the effect of a commercially available, phosphate-restricted, diet on urinary TGF-β1 excretion in cats with CKD; and (b) the role of increased extracellular phosphate in regulating proliferation, apoptosis, and expression of genes related to TGF-β1 signalling and extracellular matrix (ECM) production in feline proximal tubular epithelial cells (FPTEC) and cortical fibroblasts from cats with azotaemic CKD (CKD-FCF). The dietary intervention study revealed no effect of dietary phosphate restriction on urinary active TGF-β1 excretion after 4-8 weeks (P=0.98), despite significantly decreasing serum phosphate (P<0.001). There was no effect of increased growth media phosphate concentration (from 0.95mM to 2mM and 3.5mM) on proliferation (P=0.99) and apoptotic activity in FPTEC (P=0.22), or expression of genes related to ECM production and the TGF-β1 signalling pathway in FPTEC and CKD-FCF (P>0.05). These findings suggest the beneficial effects of dietary phosphate restriction on progression of feline CKD may not occur through modulation of renal TGF-β1 production, and do not support a direct pro-fibrotic effect of increased extracellular phosphate on feline renal cells., (Copyright © 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.)

The Crandell-Rees Feline Kidney Cell (CRFK) is an immortalised cell line derived from the feline kidney that is utilised for the growth of certain vaccinal viruses. Confusion exists as to whether CRFK are epithelial or mesenchymal in phenotype. The aim of this study was to characterise CRFK cells via immunofluorescence, enzyme cytochemistry, western blotting, RT-qPCR for S100A4 and comparison to primary feline proximal tubular epithelial cells (FPTEC) and feline cortical fibroblasts (FCF). CRFK cells were of fusiform morphology and appeared similar to FCF. CRFK expressed the mesenchymal intermediate filament (IF) protein vimentin together with two cell adhesion molecules associated with feline fibroblasts (CD29 and CD44), and lacked expression of the epithelial IF cytokeratin, myogenic IF desmin and endothelial marker von Willebrand factor (vWF). In addition, CRFK did not demonstrate brush border enzyme activity typical of FPTEC. S100A4 gene expression, implicated in both neoplastic transformation and epithelial to mesenchymal transition, was highly upregulated in CRFK in comparison to the primary feline renal cells. CRFK appear phenotypically similar to fibroblasts, rather than tubular epithelial cells, and may have undergone neoplastic transformation or epithelial-to-mesenchymal transition after extensive passaging. This finding may have potential implications for future research utilising this cell line., (Copyright © 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.)

The cytokine transforming growth factor beta 1 (TGF-β1) has been widely implicated in the development and progression of renal fibrosis in chronic kidney disease (CKD) in humans and in experimental models. The aims of this study were to assess the association between urinary active TGF-β1 and (a) development of CKD in a cross-sectional study, (b) deterioration of renal function over 1 year in a longitudinal study, and (c) renal histopathological parameters in cats. A human active TGF-β1 ELISA was validated for use in feline urine. Cross-sectional analysis revealed no significant difference in urinary active TGF-β1:creatinine ratio (aTGF-β1:UCr) between groups with differing renal function. Longitudinally, non-azotaemic cats that developed CKD demonstrated a significant (P = 0.028) increase in aTGF-β1:UCr approximately 6 months before the development of azotaemia, which remained elevated (P = 0.046) at diagnosis (approximately 12 months prior, 8.4 pg/mg; approximately 6 months prior, 22.2 pg/mg; at CKD diagnosis, 24.6 pg/mg). In the histopathology study, aTGF-β1:UCr was significantly higher in cats with moderate (P = 0.02) and diffuse (P = 0.005) renal fibrosis than in cats without fibrosis. Cats with moderate renal inflammation had significantly higher urinary active aTGF-β1 concentrations than cats with mild (P = 0.035) or no inflammatory change (P = 0.004). The parameter aTGF-β1:UCr was independently associated with Log urine protein:creatinine ratio in a multivariable analysis of clinicopathological parameters and interstitial fibrosis score in a multivariable analysis of histopathological features. These results suggest that urinary aTGF-β1 reflects the severity of renal pathology. Increases in urinary aTGF-β1 followed longitudinally in individual cats may indicate the development of CKD., (Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Background: It is known that use of hormone replacement therapy (HRT) by postmenopausal women increases the risk of breast cancer., Method: In this study, oestrogen receptor (ER)-alpha expression is examined using standard immunoperoxidase technique., Results: Normal breast samples of 11 Australian postmenopausal women have been included in the ER-alpha study; the result showed a strong correlation (r(2) = 0.80) between ER-alpha expression in normal breast epithelial cells and body mass index (BMI) in normal women who currently use HRT., Conclusion: This finding confirms that the possibility of increased risk of breast cancer associated with increased ER-alpha expression in normal breast epithelial cells, in turn associated with high BMI and the use of HRT.

To date, there have been no reports on the use of propranolol in electroconvulsive therapy (ECT)-induced migraine; we describe a 32-year-old woman who was successfully treated with propranolol for this condition. Over a course of ECT, the patient developed increasingly severe migraine which was refractory to treatment with acetaminophen, codeine, and naproxen. Sumatriptan did not relieve the headache and aggravated the nausea. Successful migraine relief was achieved with a combination of propranolol and naproxen, administered before and after ECT. Propranolol reduced blood pressure and decreased the heart rate, measured before and immediately after ECT. Propranolol, possibly in combination with naproxen, may be useful in both acute and prophylactic treatment of post-ECT migraine.

It is hypothesized that the human homologue of the mouse mammary tumour virus (HHMMTV) and other viruses, such as human papillomavirus (HPV) and Epstein-Barr virus (EBV), act as cofactors with diet, oestrogens and other hormones in the initiation and promotion of some types of breast cancer in genetically susceptible women. It is further hypothesized that diet influences the risk of breast cancer, through its influence on oestrogen metabolism and that of other hormones, in combination with genetic and infectious agents.

The aim of this ecological study was to explore possible associations between childhood nutrition and breast cancer. The study compared heights and weights of children of different socioeconomic status and subsequent breast cancer risk in Queensland, Australia. In 1950 12-year-old girls from families of high socioeconomic status were 3 cm taller and 6.6 kg heavier than girls of the same age of lower socioeconomic status. Approximately 35 years later, age standardized mortality rates for breast cancer among all Queensland women were approximately 10% higher for women of high compared with low socioeconomic status. Taking into account the limitations of ecological studies and risk factors other than nutrition, these results are compatible with the hypothesis that there is an association between childhood nutritional experiences and subsequent risk of breast cancer.

Objective: To determine the frequency and circumstances of serious cervical cord injuries associated with rugby union and league football in New South Wales., Design: Retrospective review of patients with rugby football-related cervical spinal cord injuries., Setting: The two central spinal units in NSW, from January 1984 to July 1996., Outcome Measures: Admission to spinal units; injury resulting in permanent tetraplegia., Results: During the review period, 115 rugby football players (56 union and 59 league) were admitted to the spinal units because of cervical spinal cord injuries. 49 patients had resultant permanent neurological deficits (complete tetraplegia [quadriplegia])--26 associated with rugby union and 23 with rugby league. Two patients died of injury sequelae within two weeks of admission. There was no significant change in the rate of football-related admissions to spinal units for either code. There was a small decline in the number (from 15 in 1984 to 1987 to 7 in 1992 to 1996) and incidence (from 1.2 to 0.5 per 10,000 participants) of patients with tetraplegia associated with rugby union. When this decline was tested as a trend over the years, it was found to be statistically significant (P = 0.06). No significant trend was found in the tetraplegia data associated with rugby league. Cervical spinal cord injuries leading to complete tetraplegia were most commonly associated with scrum-like plays in union and with tackles in league., Conclusion: Serious cervical spinal injuries associated with both codes of rugby continue to occur in NSW. Rugby football in its various forms is still an inherently dangerous game.

The study was designed to test whether topographic map data expressed in log power units have a multivariate normal (MVN) distribution in a healthy population, and to determine if any deviation from normality poses serious difficulties for the use of standard multivariate statistical tests in assessing the significance of deformations of the map in individual cases. Data on 361 healthy adults using 20 electrodes in the classic 10/20 configuration were recorded in six frequency bands. The log-transformed power data were shown to deviate markedly from MVN. The actual distributions of multivariate tests were computed for the sample using the 'jackknife' method, and shown to deviate markedly from the F-distributions that would be expected for MVN data. These 'jackknife' sampling distributions were then used to demonstrate significant deformations in the topographic maps of a patient who had sustained traumatic head injury.

Mainly because of migration of young people, the Australian population is young in comparison with the population of other economically developed countries. Fragmentation and excessive reliance on institutions of care of handicapped and elderly people have led to efforts at coordination of services, quality assurance, and a balance between institutional and community-based services.

Objective: To report the occurrence of uncommon but catastrophic eye and testicle injuries to rugby league and rugby union footballers in New South Wales., Methods: A retrospective review of players who had permanent football-related injuries and who claimed financial compensation from a voluntary sports injury insurance scheme from 1978-1994., Results: An average of 100 000 rugby league and rugby union players per year were registered with the insurance scheme in NSW. As a consequence of playing football, 15 players lost 90%-100% vision in one eye, five lost 75% vision in one eye, four lost 50% of vision in one eye and two lost 75% vision in both eyes. Eye injuries were caused by "gouging" with fingers, kicks, or blows from fists, elbows and knees; five cases appeared to be intentional. Fourteen players had testicular injuries: in 11 players this resulted in complete loss of one testicle, and in three partial loss of one or both testicles. The immediate cause of the injuries to the testicles was kicking and kneeing, usually during tackles; at least three of the injuries appeared to be intentional., Recommendations: Rules relating to tackles, kicks and other assaults to the head or groin should be strengthened and policed. Officials, players, parents and the public should be educated to support this.

Objective: This study attempted to estimate the prevalence of dissociative symptoms and disorders in a Canadian adult psychiatric inpatient population and also attempted to determine the extent to which dissociative disorders were recognized by the attending clinical staff., Method: All appropriate and consenting adult psychiatric inpatients at the Kingston Psychiatric Hospital in Kingston, Ontario, were given the Dissociative Experiences Scale. Patients scoring 25 or greater were interviewed with the Dissociative Disorders Interview Schedule and the Structured Clinical Interview for DSM-IV Dissociative Disorders. Admission or discharge diagnoses data were used to determine whether or not dissociative disorders were being recognized., Results: A total of 48 patients completed the Dissociative Experiences Scale and 14 (29%) scored 25 or greater. The prevalence of dissociative disorders in this hospital population was estimated to be 17%. Dissociative identity disorder was found in six percent, dissociative amnesia in eight percent and dissociative disorder not otherwise specified in two percent of the population. These disorders tended to be under-recognized., Conclusion: Research on more extensive populations is required to establish the true prevalence of dissociative symptoms and disorders in psychiatric inpatients.

This article reports the results of a study which was designed to evaluate a revised Master's of Health Administration program in the School of Health Services Management at the University of New South Wales, Australia. The original program was established in 1956; during the 1970s, humanities-based subjects such as sociology and quality assurance were added to the traditional financial and management subjects. During 1987-88, health-oriented subjects such as public health and epidemiology were included as compulsory subjects. To assess the program, a cross-sectional study of 1990 to 1992 graduates was undertaken. The study revealed that students found that the traditional financial and management subjects and the health-related subjects were useful to their jobs. The study also demonstrated significant career advancement for a majority of graduates. Two-thirds of the respondents stated that their initial objectives for enrolling in the course had been met.

This paper compares costs for caring for patients according to common diagnosis groups in Australian public teaching, public non-teaching and private hospitals. Generally, the costs for general surgical procedures are highest in public teaching hospitals, followed by public non-teaching hospitals, and are lowest in private hospitals. However, the private sector is more expensive than the public sector for obstetric activities. The reasons for the differences appear to be the much higher 'overheads' in the public sector than in the private sector, and the longer hospital stay for obstetric patients in private hospitals. Managers of individual hospitals should examine the data in detail to determine if alternative approaches are appropriate.

Medical clinicians have for many years been managers. However, this management role has most often been in groups of other clinicians and other health personnel. Only recently have increasing numbers of medical clinicians been asked to accept responsibilities for managing hospital and health services divisions that carry financial and administrative responsibility in addition to traditional clinical responsibility. In these circumstances, it is appropriate to seek to gather information about the various issues that surround the transition from clinician to manager in a systematic way and to begin to explore the issue and any actions that might be taken to ameliorate transitional problems. Such a study was undertaken by the School of Health Services Management at the University of New South Wales, Sydney, Australia, and is reported in this article.

Much of the confusion surrounding international comparisons of infant and perinatal mortality rates could be removed if single definitions of live birth and fetal death were universally adopted. Suggestions as to how this might be achieved are made below.

International experiences are becoming more relevant as the Clinton Administration searches for answers to America's health care problems. While interest is focused on the Canadian and European experiences, a brief look at far away Australia offers some potentially useful insights.

This article analyses Medicare payments to doctors, both general practitioners and specialists. Although these payments represented approximately $4.5 billion in 1991-92, there has been little public analysis of the breakdown of these payments to different medical specialties and general practitioners. These payments are just one source of income for many doctors. Information on practice costs is also presented to give an indication of the size of doctors' net incomes. A comparison is also made between doctors' incomes and taxpayers in general.

As an incidental finding in a general 'life-style' survey, amateur rugby footballers were found to drink prodigious (12-24 schooners per session) quantities of beer. Such drinking was regarded by the players as part of the traditions of mateship associated with the game. The need for intervention is discussed.

There is a clear link between social relationships and health in terms of both morbidity and mortality. The study was conducted during 1989 and 1990 among mothers of newborn infants from a middle-ranking socioeconomic status suburb in Sydney (Ryde). Thirty-four per cent of the study group had a moderate level of social isolation and 16 per cent had a high level of social isolation. These mothers strongly valued participation in the groups: virtually all original participants remained with the group both during a five-week formal period with a nursing staff member as the leader and during months and up to five years of continued meetings. The outcomes appear to be favourable in terms of the development and continuation of strong interpersonal relationships among members of the group and by improved confidence and knowledge about infant rearing. The development of groups of mothers adds no cost to the provision of health services and provides a sound and practical way of reducing social isolation and increasing mothercraft skills in an Australian suburb.

During the past 15 years the district health services in some parts of Australia have been reorganized, with the development of coordinated programmes that provide services across hospital and community boundaries as well as continuity of care over prolonged periods. Some financial resources have been redirected from institutions to community-based services. The process has been so successful that district reorganization has now been implemented throughout New South Wales (population, 5.5 million) and elsewhere in Australia.

Despite many difficulties and setbacks, China has made immense progress in health development during the past four decades. A continuing determination to reach all the people with preventive measures, primary care and hospital facilities should ensure that success is maintained in this field.

A meta-analysis of the controlled clinical trials comparing the efficacy of combined lithium-imipramine therapy with each drug alone in the prevention of relapse in patients with unipolar depressive illness shows the combination to be superior to lithium alone (for any relapse, p less than 0.05; for specifically depressive relapse, p less than 0.025) and imipramine alone (for any relapse, p less than 0.025; for specifically depressive relapse, p less than 0.05). The issue of statistical power in studies of this kind is discussed. A single case of unipolar depressive illness is described, in which combined lithium-tranylcypromine therapy was superior to either drug alone. A combination of lithium and an antidepressant should be considered for any patient suffering from this condition who fails to remain well on either drug alone.

There has been a dramatic improvement in the dental health of school children in the past 15 years. The proportion of children aged five to 12 years who have never experienced any dental decay increased nearly ten-fold from 3.8% in 1961 to over 35% in 1974. The proportion of children with active dental decay at routine school examinations has fallen from 92% to 22%. These improvements are probably due to improved oral hygiene and diet in combination with the usage of fluoride. Almost certainly, the availability of fluoride both in tablet form and delivered through town water supplies has been the predominant factor. These very large reductions in the incidence of dental decay amont children represent a modern triumph of preventive health care. Further improvements in dental health may be effected by the topical application of fluoride and reduced intake of refined sugar.

A study of the caries experience of 12-year-old children attending public high schools in the northern suburbs of Sydney was undertaken in 1982. The data obtained were compared with those from a similar study conducted in the same area in 1963. In the period between the two examinations, Sydney's water supply was fluoridated and fluoride toothpastes became widely available. There was a major improvement in dental health in the 19-year interval between the surveys. The mean number of decayed, missing, and filled teeth (DMFT) per child declined from 8.49 in 1963 to 1.37 in 1982, a reduction of 84%. An analysis of the DMFT index showed that the greatest reductions occurred in the decayed tooth (DT), and missing tooth (MT) components (95% and 94%, respectively). In 1982, in contrast to the situation in 1963, the backlog of unmet restorative dentistry needs was low.

The impact of cerebrovascular accident (CVA) on sexual behavior in stroke patients was studied in 113 patients (78 men, 35 women). Seventy-five patients were interviewed with their spouses present. The mean age of the men and women patients was 68.6 years and 68.0 years respectively. Significant decline in libido after stroke was reported by both men and women. The men had a significant decrease in ability to achieve erection and to ejaculate in the period after stroke. Similarly, significant problems were reported by the women regarding normal vaginal lubrication and orgasm after CVA. Sixty-six men (84%) and 21 women (60%) enjoyed their sex lives before their stroke as compared to only 23 men (30%) and 11 women (31%) after stroke. Seventy-four men (95%) and 27 women (76%) were satisfied with sexual activity before their stroke as compared to only 20 men (26%) and 13 women (37%) after stroke. Women patients with right-sided lesions had lesser decline in sexual function than women with left-sided lesions or men with either right or left hemispheric lesions. The most common factor identified as causing decline in sexual activity was the fear that having sex might adversely affect blood pressure and cause another stroke. The sexual problems of these patients are of sufficient magnitude and frequency to warrant further investigation of the physiologic effects of sexual activity on blood pressure and cardiac function after stroke.

Little information is available on the health status of persons 85 years or over. Recent United States data indicate that 20% of individuals 85 years of age or over reside in nursing and personal care homes and that among these institutional residents 31% are bedfast, 11% are chairfast and 71% manifest evidence of senility. An investigation into the health of persons 85 years of age or over in two Ontario counties revealed that 39% resided in long-stay institutions, and that one of the main differences between old people living independently in the community and those in institutions was the higher proportion of the latter needing help with the activities of daily living and showing mental disorientation. Of those individuals 85 years of age or over in institutions for 1 year, 26% acknowledged often feeling depressed and 18% acknowledged often wishing they were dead, but these tendencies were not more frequent in those 85 years of age or over than in those 65 to 84 years of age. These findings have implications for service and research needs and attitudes towards death.