101. Lateral root emergence in Arabidopsis is dependent on transcription factor LBD29 regulating auxin influx carrier LAX3
- Author
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Anthony Bishopp, Benjamin Péret, Britta Kuempers, Ben Scheres, Kristine Hill, Ranjan Swarup, Siobhan M. Brady, Malcolm J. Bennett, Hidehiro Fukaki, Allison Gaudinier, Yujuan Du, Julien Lavenus, Silvana Porco, Ilda Casimiro, Antoine Larrieu, Kamal Swarup, Eva Benková, Tatsuaki Goh, Centre for Plant Integrative Biology, University of Nottingham, Reproduction et développement des plantes (RDP), École normale supérieure - Lyon (ENS Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Department of Biology, Molecular Genetics, Faculty of Science, Utrecht University [Utrecht], Department of Plant Biology and Genome Center, University of California [Davis] (UC Davis), University of California, Kobe University, Graduate School of Science, Institute of Plant Sciences, Biologia Celular Y Zoologia, Universidad de Extremadura (UEX), Facultad de Ciencias (UDELAR), Institute of Science and Technology Austria, Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), University of California (UC), Konan University [Kobe, Japan], Universidad de Extremadura - University of Extremadura (UEX), Institute of Science and Technology [Klosterneuburg, Austria] (IST Austria), Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institute of Science and Technology [Austria] (IST Austria), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Péret, Benjamin, Bennett, Malcolm J, Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon)
- Subjects
0301 basic medicine ,Auxin influx ,Auxin efflux ,lateral root emergence ,root development ,580 Plants (Botany) ,racine laterale ,Plant Roots ,LBD29 ,03 medical and health sciences ,Auxin ,Gene Expression Regulation, Plant ,arabidopsis ,auxin ,Arabidopsis ,Botany ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,Primordium ,heterocyclic compounds ,développement racinaire ,Molecular Biology ,Transcription factor ,chemistry.chemical_classification ,Vegetal Biology ,biology ,Indoleacetic Acids ,auxine ,Arabidopsis Proteins ,Lateral root ,fungi ,Membrane Transport Proteins ,food and beverages ,biology.organism_classification ,Cell biology ,Pericycle ,030104 developmental biology ,chemistry ,Biologie végétale ,Developmental Biology ,Signal Transduction ,Transcription Factors - Abstract
Lateral root primordia (LRP) originate from pericycle stem cells located deep within parental root tissues. LRP emerge through overlying root tissues by inducing auxin-dependent cell separation and hydraulic changes in adjacent cells. The auxin inducible auxin influx carrier LAX3 plays a key role concentrating this signal in cells overlying LRP. Delimiting LAX3 expression to two adjacent cell files overlying new LRP is critical to ensure auxin-regulated cell separation occurs solely along their shared walls. Multiscale modeling has predicted this highly focused pattern of expression requires auxin to sequentially induce auxin efflux and influx carriers PIN3 and LAX3, respectively. Consistent with model predictions, we report that LAX3 auxin inducible expression is regulated indirectly by the AUXIN RESPONSE FACTOR ARF7. Yeast-1-hybrid screens revealed the LAX3 promoter is bound by the transcription factor LBD29, which is a direct target for regulation by ARF7. Disrupting auxin inducible LBD29 expression or expressing an LBD29-SRDX transcriptional repressor phenocopied the lax3 mutant, resulting in delayed lateral root emergence. We conclude that sequential LBD29 and LAX3 induction by auxin is required to coordinate cell separation and organ emergence.
- Published
- 2016