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ECHIDNA-mediated post-Golgi trafficking of auxin carriers for differential cell elongation
- Source :
- Proceedings of the National Academy of Sciences of the United States of America, Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2013, 110 (40), pp.16259-16264. ⟨10.1073/pnas.1309057110⟩
- Publication Year :
- 2013
-
Abstract
- The plant hormone indole-acetic acid (auxin) is essential for many aspects of plant development. Auxin-mediated growth regulation typically involves the establishment of an auxin concentration gradient mediated by polarly localized auxin transporters. The localization of auxin carriers and their amount at the plasma membrane are controlled by membrane trafficking processes such as secretion, endocytosis, and recycling. In contrast to endocytosis or recycling, how the secretory pathway mediates the localization of auxin carriers is not well understood. In this study we have used the differential cell elongation process during apical hook development to elucidate the mechanisms underlying the post-Golgi trafficking of auxin carriers in Arabidopsis. We show that differential cell elongation during apical hook development is defective in Arabidopsis mutant echidna (ech). ECH protein is required for the trans-Golgi network (TGN)-mediated trafficking of the auxin influx carrier AUX1 to the plasma membrane. In contrast, ech mutation only marginally perturbs the trafficking of the highly related auxin influx carrier LIKE-AUX1-3 or the auxin efflux carrier PIN-FORMED-3, both also involved in hook development. Electron tomography reveals that the trafficking defects in ech mutant are associated with the perturbation of secretory vesicle genesis from the TGN. Our results identify differential mechanisms for the post-Golgi trafficking of de novo-synthesized auxin carriers to plasma membrane from the TGN and reveal how trafficking of auxin influx carriers mediates the control of differential cell elongation in apical hook development.
- Subjects :
- 0106 biological sciences
Electron Microscope Tomography
EFFLUX
[SDV]Life Sciences [q-bio]
Arabidopsis
Vesicular Transport Proteins
Golgi Apparatus
01 natural sciences
Cell membrane
heterocyclic compounds
NETWORK
chemistry.chemical_classification
0303 health sciences
APICAL HOOK DEVELOPMENT
Multidisciplinary
Plant Stems
Membrane transport protein
IAA
food and beverages
Biological Sciences
ETHYLENE
Cell biology
Transport protein
Protein Transport
medicine.anatomical_structure
GROWTH
sorting
Auxin influx
morphogenesis
Biology
Cell Enlargement
Endocytosis
03 medical and health sciences
Auxin
medicine
Secretion
Secretory pathway
030304 developmental biology
Indoleacetic Acids
Arabidopsis Proteins
fungi
Cell Membrane
PIN
Membrane Transport Proteins
TRANSPORT
chemistry
biology.protein
ARABIDOPSIS-THALIANA
ATPASE ACTIVITY
010606 plant biology & botany
ROOT GRAVITROPISM
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 110
- Issue :
- 40
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Accession number :
- edsair.doi.dedup.....9fcc7417b4cb2cd98974c953fb963a3e