1,282 results on '"Hiroyuki Inoue"'
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102. Delineating Karsts, Small-Scale Faults, and Fractures by Using a Global Stratigraphic Framework to Integrate Conventional Seismic Attributes with Diffraction Imaging in a Giant Offshore Field, Abu Dhabi
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Yassar Goraya, Ali Al Felasi, Nicolas Daynac, Stuart Walley, Marc-Antoine Dupont, Hiroyuki Inoue, Ahmed Mubarak Al Khamiri, and Alia Hasan Hindi
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Poor resolution and signal-to-noise ratio have often been key factors impacting an interpreter’s ability to directly delineate subsurface features from seismic data. Improvements in full azimuth, high-density acquisition and in diffraction imaging, have the potential to reveal greater subsurface detail. To date, the application of diffraction imaging has in part been limited by the methods available to parse and analyze that same data. For instance, visualization of diffraction images on time and or depth slices may show patterns, but as these slices can cut through successive seismic reflectors, they tend not to be geologically meaningful. An approach is described that uses machine automation to rapidly incorporate diffraction images into a full-volume 3D seismic interpretation. Delineation of key stratigraphic surfaces is driven by stacking patterns and stratigraphic terminations and performed in both structural and Wheeler domains. Stratal slicing is a highly flexible and rapid method of generating chronostratigraphic surfaces. These chronostratigraphic surfaces can be extracted at sub-sample resolution and therefore accurately matched to well log responses which typically fall well below the resolution of a seismic dataset. The diffraction image is then projected onto a series of chronostratigraphic surfaces, allowing the interpreter to parse through and compare diffraction data directly with conventional seismic attributes at the same chronostratigraphic layer. The novel approach described has been used to demonstrate both the value of diffraction imaging and the importance of using a global full volume 3D seismic interpretation when identifying features such as karsts and small-scale faults and fractures. When applied to a recently processed high-density wide azimuth seismic survey, the workflow was able to seamlessly integrate diffraction images to provide improved confidence in the delineation of karsts and other collapse features that can pose a drilling hazard within the Giant Field, offshore United Arab Emirates.
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- 2022
103. Human-Induced Pluripotent Stem Cell–Derived Cardiomyocyte Model for TNNT2 Δ160E-Induced Cardiomyopathy
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Takumi Kondo, Shuichiro Higo, Mikio Shiba, Yasuaki Kohama, Satoshi Kameda, Tomoka Tabata, Hiroyuki Inoue, Shota Okuno, Shou Ogawa, Satoki Nakamura, Maki Takeda, Emiko Ito, Junjun Li, Li Liu, Yuki Kuramoto, Jong-Kook Lee, Seiji Takashima, Shigeru Miyagawa, Yoshiki Sawa, Shungo Hikoso, and Yasushi Sakata
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General Medicine - Abstract
Background: The Δ160E mutation in TNNT2 , which encodes troponin T, is a rare pathogenic variant identified in patients with hypertrophic cardiomyopathy and is associated with poor prognosis. Thus, a convenient human model recapitulating the pathological phenotype caused by TNNT2 Δ160E is required for therapeutic development. Methods: We identified a heterozygous in-frame deletion mutation (c.478_480del, p.Δ160E) in TNNT2 in a patient with familial hypertrophic cardiomyopathy showing progressive left ventricular systolic dysfunction, leading to advanced heart failure. To investigate the pathological phenotype caused by Δ160E, we generated a set of isogenic induced pluripotent stem cells carrying the heterozygous Δ160E, homozygously corrected or homozygously introduced Δ160E using genome editing and differentiated them into cardiomyocytes (Hetero-Δ160E-, wild type-, and Homo-Δ160E-induced pluripotent stem cells [iPSC]-derived cardiomyocytes [iPSC-CMs]). Results: Hetero-Δ160E-iPSC-CMs exhibited prolonged calcium decay, relaxation impairment, and hypertrophy compared to wild type-iPSC-CMs. Notably, these phenotypes were further exacerbated in Homo-Δ160E-iPSC-CMs. Overexpression of R-GECO-fused Δ160E mutant troponin T prolonged decay time and time to peak of the myofilament-localized calcium transient in iPSC-CMs, indicating that sarcomeric calcium retention with Δ160E may affect intracellular calcium concentration. High-content imaging analysis detected remarkable nuclear translocation of NFATc1, especially in Homo-Δ160E-iPSC-CMs, indicating that the Δ160E mutation promotes hypertrophic signaling pathway in a dose-dependent manner. Increased phosphorylation of CaMKIIδ (calcium/calmodulin-dependent protein kinase IIδ) and phospholamban at Thr17 was observed in Homo- and Hetero-Δ160E-iPSC-CMs. Epigallocatechin-3-gallate, a calcium desensitizing compound, shortened prolonged calcium decay and relaxation duration in Δ160E-iPSC-CMs. Conclusions: Isogenic iPSC-CMs recapitulate the prolonged calcium decay, relaxation impairment, and subsequent calcium-regulated signaling pathways caused by the TNNT2 Δ160E mutation and can serve as a human model for therapeutic development to prevent hypertrophic cardiomyopathy pathology.
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- 2022
104. An ETL Framework for Online Analytical Processing of Linked Open Data.
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Hiroyuki Inoue, Toshiyuki Amagasa, and Hiroyuki Kitagawa
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- 2013
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105. A control system for electrical appliances using eye-gaze input.
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Hidenobu Nakayama, Noboru Yabuki, Hiroyuki Inoue, Yasuaki Sumi, and Takao Tsukutani
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- 2012
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106. Hypothermia causes platelet activation in the human spleen
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Kanar Alkass, Ayumi Motomura, Namiko Ishii, Keisuke Okaba, Hiroki Tanaka, Hiroyuki Inoue, Daisuke Yajima, Shinnosuke Yamada, Henrik Druid, and Kie Horioka
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Blood Platelets ,Pathology ,medicine.medical_specialty ,Spleen ,Hypothermia ,Fibrin ,Mice ,Animals ,Humans ,Medicine ,Platelet ,Platelet activation ,Coagulation Disorder ,biology ,business.industry ,Endothelial Cells ,Hematology ,Platelet Activation ,medicine.anatomical_structure ,Coagulation ,Splenic Tissue ,biology.protein ,medicine.symptom ,business - Abstract
Background Accidental hypothermia results in various dysfunctions in the human body. Additionally, coagulation disorder can lead to a life-threatening condition. We previously demonstrated that platelets stored in the spleen were activated and thus triggered coagulation disorder in a mouse model of hypothermia. In the present study, we wanted to investigate if this phenomenon in mice also occurs in humans as a reaction to hypothermia. Methods We analyzed splenic tissue collected from 22 deceased subjects who have died from hypothermia. These samples were compared with 22 control cases not exposed to cold environment. We performed immunohistochemical staining for CD61 (a marker of all platelets) and CD62P (a marker of activated platelets). We also evaluated the morphology of platelets in the spleen with scanning electron microscopy. Results Immunohistochemical analysis revealed no significant changes in the amounts of CD61-positive platelets between the hypothermia and control cases. However, the hypothermia cases contained abundant CD62P-positive platelets compared with those of the control cases. Immunohistochemical analysis also revealed that the activated platelets formed aggregates and adhered to splenic sinusoidal endothelial cells in the hypothermia cases. However, we observed no significant fibrin formation around the activated platelets. Conclusions Hypothermia resulted in splenic platelet activation, which may be used as a postmortem marker of hypothermia. The release of activated platelets from the spleen into to circulation upon rewarming may promote coagulation disturbances.
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- 2021
107. The potent protein phosphatase 2A inhibitors aminocytostatins: new derivatives of cytostatin
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Shigehiro Tohyama, Masayuki Igarashi, Kazuaki Matoba, Ryuichi Sawa, Hiroyuki Inoue, Hideyuki Muramatsu, Manabu Kawada, and Masaki Hatano
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Cell Survival ,Protein Conformation ,Streptomycetaceae ,Antineoplastic Agents ,Inhibitory postsynaptic potential ,Mice ,Structure-Activity Relationship ,Cytostatin ,In vivo ,Cell Line, Tumor ,Drug Discovery ,Animals ,Moiety ,Protein Phosphatase 2 ,Pharmacology ,chemistry.chemical_classification ,Molecular Structure ,Chemistry ,Protein phosphatase 2 ,Organophosphates ,In vitro ,Molecular Docking Simulation ,Biochemistry ,Pyrones ,Docking (molecular) ,Lactone ,Protein Binding - Abstract
Specific inhibitors of protein phosphatase 2A (PP2A) mediate anticancer effects by augmenting the tumor-killing activity of natural killer (NK) cells. In this study, new PP2A inhibitors, aminocytostatins A-E, were isolated from Kitasatospora sp. MJ654-NF4 and structurally characterized. Aminocytostatins are derivatives of cytostatin, which is a specific PP2A inhibitor isolated from the same organism, and aminocytostatins have a characteristic amino group within the lactone moiety. Compared to cytostatin, aminocytostatin A showed a stronger inhibitory activity against PP2A in vitro and augmented the tumor-killing activity of NK cells in vivo. Furthermore, a docking model was generated to demonstrate the favorable activities of aminocytostatin A.
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- 2021
108. Activation of the intestinal tissue renin-angiotensin system by transient sodium loading in salt-sensitive rats
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Masaki Ryuzaki, Asuka Uto, Hiroyuki Inoue, Hiroshi Itoh, Aika Hagiwara, Masaaki Sato, Kenichiro Kinouchi, Takuma Oshida, Kentaro Fujii, Kazutoshi Miyashita, and Sho Endo
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medicine.medical_specialty ,Physiology ,Sodium ,chemistry.chemical_element ,Blood Pressure ,Renin-Angiotensin System ,Rats, Inbred SHR ,Internal medicine ,Renin ,Renin–angiotensin system ,Internal Medicine ,medicine ,Animals ,Sodium Chloride, Dietary ,Receptor ,intestine ,salt absorption ,business.industry ,Angiotensin II ,sodium/hydrogen exchanger ,tissue renin-angiotensin system ,Original Articles ,salt-sensitive hypertension ,Small intestine ,Rats ,Endocrinology ,Blood pressure ,medicine.anatomical_structure ,Valsartan ,chemistry ,Hypertension ,Cardiology and Cardiovascular Medicine ,business ,Homeostasis ,medicine.drug - Abstract
Background The renal tissue renin--angiotensin system is known to be activated by salt loading in salt-sensitive rats; however, the response in other organs remains unclear. Method Spontaneously hypertensive rats were subjected to normal tap water or transient high-salt-concentration water from 6 to 14 weeks of age and were thereafter given normal tap water. From 18 to 20 weeks of age, rats given water with a high salt concentration were treated with an angiotensin II type 1 receptor blocker, valsartan. Results Sustained blood pressure elevation by transient salt loading coincided with a persistent decrease in the fecal sodium content and sustained excess of the circulating volume in spontaneously hypertensive rats. Administration of valsartan sustainably reduced the blood pressure and normalized the fecal sodium levels. Notably, transient salt loading persistently induced the intestinal tissue renin--angiotensin system and enhanced sodium transporter expression exclusively in the small intestine of salt-sensitive rats, suggesting the potential connection of intestinal sodium absorption to salt sensitivity. Conclusion These results reveal the previously unappreciated contribution of the intestinal tissue renin--angiotensin system to sodium homeostasis and blood pressure regulation in the pathophysiology of salt-sensitive hypertension.
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- 2021
109. A threat to mobile devices from spoofing public USB charging stations.
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Tadashi Shiroma, Yasuhiko Nishio, and Hiroyuki Inoue
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- 2017
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110. Development of a Digital Signage System for Automatic Collection and Distribution of Its Content from the Existing Digital Contents and Its Field Trials.
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Hiroyuki Inoue, Kaoru Suzuki, Koji Sakata, and Kaori Maeda
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- 2011
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111. A Handover Control of Mobile Nodes with IP Mobility in Heterogeneous Wireless Networks.
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Takuya Horai, Yuki Iwata, Hayato Morihiro, Yasuhiro Ohishi, Kaori Maeda, and Hiroyuki Inoue
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- 2011
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112. Bounded Model Checking of Concurrent Systems with Unbounded Integer Variables.
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Hiroyuki Inoue, Tatsuhiro Tsuchiya, and Tohru Kikuno
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- 2011
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113. Mutant forms of EGFR promote HER2 trafficking through efficient formation of HER2-EGFR heterodimers
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Hirono Tsutsumi, Eiji Iwama, Ritsu Ibusuki, Atsushi Shimauchi, Keiichi Ota, Yasuto Yoneshima, Hiroyuki Inoue, Kentaro Tanaka, Yoichi Nakanishi, and Isamu Okamoto
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Pulmonary and Respiratory Medicine ,Cancer Research ,Oncology - Abstract
Human epidermal growth factor receptor 2 (HER2) forms homodimers and is retained at the surface of cancer cells positive for HER2 amplification. The dimerization, internalization, and intracellular trafficking of HER2 in cancer cells without HER2 amplification have remained uncharacterized, however.HER2 homodimers and heterodimers were detected in various cell lines with the use of an in situ proximity ligation assay. The effects of wild-type or mutant forms of epidermal growth factor receptor (EGFR) on intracellular trafficking of HER2 were examined by live-cell imaging. The sensitivity of cell lines without HER2 amplification to ado-trastuzumab emtansine (T-DM1), an anti-HER2 (trastuzumab)-cytotoxic drug conjugate (ADC) was also investigated.HER2 preferentially formed heterodimers with EGFR rather than homodimers and was rapidly internalized together with EGFR in cells without HER2 amplification. HER2-EGFR heterodimers were more abundant and HER2 was more efficiently transferred to lysosomes in such cells with than in those without EGFR activating mutations. T-DM1 showed a high cytotoxic efficacy in the cells with EGFR mutations, suggesting that mutant forms of EGFR promote the transfer of HER2-bound T-DM1 to lysosomes through efficient formation of HER2-EGFR heterodimers.Our findings reveal that HER2 trafficking is affected by EGFR, especially by mutant forms of the receptor, and they provide a rationale for the use of HER2-targeting ADCs in the treatment of EGFR-mutated lung cancer.
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- 2022
114. ASO Visual Abstract: NADPH Oxidase 2 Has a Crucial Role in Cell Cycle Progression of Esophageal Squamous Cell Carcinoma
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Hiroki Shimizu, Keita Katsurahara, Hiroyuki Inoue, Atsushi Shiozaki, Toshiyuki Kosuga, Michihiro Kudou, Tomohiro Arita, Hirotaka Konishi, Shuhei Komatsu, Hitoshi Fujiwara, Yukiko Morinaga, Eiichi Konishi, and Eigo Otsuji
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Esophagectomy ,Oncology ,Esophageal Neoplasms ,NADPH Oxidase 2 ,Cell Cycle ,Carcinoma, Squamous Cell ,Humans ,Surgery ,Esophageal Squamous Cell Carcinoma - Published
- 2022
115. Double-balloon enteroscopy-assisted polypectomy for a solitary jejunal Peutz-Jeghers polyp causing anemia
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Kazunori Onuma, Gota Sudo, Hiroyuki Inoue, Takahiro Kuriyama, Yumemi Takada, Atsushi Yawata, and Hiroshi Nakase
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Gastroenterology - Published
- 2022
116. Digital Signage with Audience Detection Using TV Broadcasting Waves.
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Kaori Maeda, Masahiro Nishi, Teruaki Yoshida, Kaoru Suzuki, and Hiroyuki Inoue
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- 2010
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117. A Design and Development of a Multi-source Streaming Platform Based on a Building Block Architecture.
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Tohru Kondo, Reiji Aibara, Kouichi Abe, Yuuiti Ishikuni, Hiroyuki Inoue, and Kaori Maeda
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- 2010
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118. Development of Upper-Limb Power-Assist Machine Using Linkage Mechanism - Mechanism and its Fundamental Motion -.
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Hiroyuki Inoue and Toshiro Noritsugu
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- 2014
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119. Oligometastasis scoring system for predicting survival of patients with colorectal liver metastasis after hepatectomy
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Hiroki Shimizu, Hideo Takahashi, Tomohiro Arita, Atsushi Shiozaki, Hisashi Ikoma, Yusuke Yamamoto, Allan Tsung, Eigo Otsuji, Hiroyuki Inoue, Hitoshi Fujiwara, Tsutomu Kawaguchi, Ryo Morimura, Kazuma Okamoto, Takeshi Kubota, Yoshiaki Kuriu, Toshiya Ochiai, Kazuaki Takabe, and Hirotaka Konishi
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Adult ,Male ,medicine.medical_specialty ,Scoring system ,Colorectal cancer ,medicine.medical_treatment ,Independent predictor ,Gastroenterology ,Metastasis ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Overall survival ,Hepatectomy ,Humans ,Aged ,Retrospective Studies ,Aged, 80 and over ,Proportional hazards model ,business.industry ,Liver Neoplasms ,Hazard ratio ,General Medicine ,Middle Aged ,Prognosis ,medicine.disease ,Survival Rate ,Oncology ,030220 oncology & carcinogenesis ,Female ,030211 gastroenterology & hepatology ,Surgery ,Colorectal Neoplasms ,business ,Software ,Follow-Up Studies - Abstract
BACKGROUND Oligometastasis, the presence of a small number of resectable metastatic tumors, usually has favorable outcomes. Here we examined whether the novel oligometastatic score (OLGS), which divides the number of colorectal liver metastases (CRLMs) by the time from colorectal resection to liver recurrence, better predicts CRLM patient survival than the commonly used clinical risk score. METHODS A total of 143 patients who underwent curative hepatectomy for CRLMs between 2007 and 2018 were analyzed. We investigated their clinical characteristics and outcomes using OLGS. RESULTS Of the 143 CRLM patients, 70 had synchronous CRLMs and 73 had metachronous CRLMs. Patients with metachronous CRLMs were divided into OLGS-low (n = 59) and OLGS-high (n = 14) subgroups. The 5-year overall survival (OS) rates after hepatectomy differed significantly between the subgroups (p
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- 2021
120. The Transcription Factor Gene tclB2 Regulates Mannanolytic Enzyme Production in the Fungus Talaromyces cellulolyticus
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Akinori Matsushika, Hiroyuki Inoue, and Tatsuya Fujii
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0106 biological sciences ,Bioengineering ,Fungus ,01 natural sciences ,Applied Microbiology and Biotechnology ,Biochemistry ,Cellulase ,Gene Expression Regulation, Fungal ,010608 biotechnology ,Transcriptional regulation ,Molecular Biology ,Gene ,Transcription factor ,chemistry.chemical_classification ,Strain (chemistry) ,biology ,010405 organic chemistry ,beta-Mannosidase ,General Medicine ,biology.organism_classification ,0104 chemical sciences ,Enzyme ,Talaromyces ,chemistry ,Transcription Factor Gene ,Function (biology) ,Biotechnology - Abstract
The filamentous fungus Talaromyces cellulolyticus is a well-characterized cellulolytic and hemicellulolytic enzyme producer. In this study, the function of the tclB2 gene, which is a homolog of the manR/clrB/clr-2 gene in other filamentous fungi, in mannanolytic enzyme production by T. cellulolyticus was investigated. When a tclB2-disrupted strain (YDTclB) was grown in the presence of glucomannan, the production of β-mannanase, β-mannosidase, and α-galactosidase was decreased at the protein and transcriptional levels when compared to the control strain. In addition, a tclB2-overexpressing strain (YHTclB) showed higher β-mannanase and β-mannosidase production. When cellulose was used as a carbon source, the expression of genes encoding mannanolytic enzymes also decreased in YDTclB. These results suggested that TclB2 contributes to mannanolytic enzyme production in T. cellulolyticus. This work is the first study to identify a transcriptional regulator of mannanolytic enzyme genes in T. cellulolyticus.
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- 2021
121. Clinical significance of left ventricular reverse remodeling after catheter ablation of atrial fibrillation in patients with left ventricular systolic dysfunction
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Yuko Hirao, Yasushi Koyama, Katsuomi Iwakura, Issei Yoshimoto, Kenshi Fujii, Koichi Inoue, Toshinari Onishi, Yuichi Ninomiya, Koji Tanaka, Masato Okada, Yasushi Sakata, Ryo Kitagaki, Hiroyuki Inoue, Atsunori Okamura, Takafumi Oka, and Nobuaki Tanaka
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medicine.medical_specialty ,Heart disease ,medicine.medical_treatment ,Diastole ,Catheter ablation ,030204 cardiovascular system & hematology ,Ventricular Function, Left ,Ventricular Dysfunction, Left ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Atrial Fibrillation ,medicine ,Humans ,Clinical significance ,030212 general & internal medicine ,Retrospective Studies ,Ejection fraction ,Ventricular Remodeling ,business.industry ,Atrial fibrillation ,Atrial Remodeling ,Odds ratio ,medicine.disease ,Treatment Outcome ,Heart failure ,Catheter Ablation ,Cardiology ,Cardiology and Cardiovascular Medicine ,business - Abstract
Left ventricular (LV) reverse remodeling (LVRR) after catheter ablation of atrial fibrillation (AFCA) has not been fully described. This study investigated the predictors and clinical outcomes of LVRR after AFCA in patients with LV systolic dysfunction.Of 3319 consecutive patients who underwent first-time AFCA between January 2012 and October 2019, 376 with a baseline LV ejection fraction of50% were retrospectively evaluated. They were subjected to 256-slice multidetector computed tomography (MDCT) scanning at baseline and 3 months after AFCA. The LVRR was defined as a decrease in the LV end-systolic volume of ≥15%.The prevalence of LVRR was 83% (n = 306). Multivariate logistic regression analysis including age, body mass index, diabetic status, beta-blocker use, and LV diastolic diameter revealed that the predictors of LVRR were non-paroxysmal atrial fibrillation (AF) (odds ratio, 2.68; 95% confidence interval, 1.42-5.05; p = 0.002) and absence of apparent underlying structural heart disease (4.81; 2.31-10.0; p0.001). The prevalence of LVRR differed depending on AF recurrence pattern prior to the post-MDCT [no episode vs. paroxysmal episode (lasting7 days) vs. persistent episode (lasting ≥7 days), 84% vs. 81% vs. 63%, respectively, p = 0.023]. During a median follow-up of 32 months, the incidence of paroxysmal form of AF recurrence was similar, whereas persistent form of AF recurrence was less frequent in patients with LVRR (10.5% vs. 18.6%, p = 0.018). Heart failure hospitalizations (2.3% vs. 15.7%, p0.001), cardiovascular deaths (0.7% vs. 4.3%, p = 0.015), and all-cause deaths (1.3% vs. 5.7%, p = 0.018) were similarly less frequent in those with LVRR.LVRR after AFCA, which was predicted by non-paroxysmal AF without any apparent structural heart disease at baseline, was associated with persistent form of AF recurrence prior to the evaluation. LVRR was associated with favorable clinical outcomes.
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- 2021
122. Evaluation of Magnesium Tin Silicide Sintered Bodies Prepared by Liquid-Phase Pressure-Less Sintering
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Masahiko Kato, Haruhiko Udono, Hiroyuki Inoue, and Takehide Kobayashi
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Materials science ,Magnesium ,Mechanical Engineering ,Metallurgy ,chemistry.chemical_element ,Liquid phase ,Sintering ,Condensed Matter Physics ,chemistry.chemical_compound ,chemistry ,Mechanics of Materials ,Silicide ,Thermoelectric effect ,General Materials Science ,Tin - Published
- 2021
123. Optical properties of novel oxyfluoride glasses on the systems of LaF 3 –LaO 3/2 –NbO 5/2 and LaF 3 –LaO 3/2 –NbO 5/2 –AlO 3/2
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Kohei Yoshimoto, Atsunobu Masuno, Yasuhiro Watanabe, Hiroyuki Inoue, and Jaeyeop Chung
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Materials science ,business.industry ,Materials Chemistry ,Ceramics and Composites ,Optoelectronics ,business ,Refractive index - Published
- 2021
124. Absolute lymphocyte count and C‑reactive protein‑albumin ratio can predict prognosis and adverse events in patients with recurrent esophageal cancer treated with nivolumab therapy
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Hiroyuki Inoue, Atsushi Shiozaki, Hitoshi Fujiwara, Hirotaka Konishi, Jun Kiuchi, Takuma Ohashi, Hiroki Shimizu, Tomohiro Arita, Yusuke Yamamoto, Ryo Morimura, Yoshiaki Kuriu, Hisashi Ikoma, Takeshi Kubota, Kazuma Okamoto, and Eigo Otsuji
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Cancer Research ,Oncology - Abstract
Predicting the prognosis and adverse events (AEs) of nivolumab therapy for recurrent esophageal cancer is very important. The present study investigated whether a simple blood biochemical examination could be used to predict prognosis and AEs following nivolumab treatment for relapse of esophageal cancer. A total of 41 patients who received nivolumab treatment for recurrent esophageal cancer after esophagectomy were analyzed. The absolute lymphocyte count (ALC), neutrophil-lymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR), monocyte-lymphocyte ratio (MLR) and C-reactive protein-albumin ratio (CAR) were assessed at the time of nivolumab induction as indices that can be calculated by blood biochemical examinations alone. Median values were 1,015 for ALC, 3.401 for NLR, 242.6 for PLR, 0.458 for MLR and 0.119 for CAR, and patients were divided into two groups according to values. A high ALC, low NLR, low PLR, low MLR and low CAR were associated with a better response to nivolumab. In addition, patients with the aforementioned indices, with the exception of low PLR, or better response were more likely to develop AEs in univariate analysis. In multivariate analysis, a high ALC [odds ratio (OR): 4.857, P=0.043] and low CAR (OR: 9.099, P=0.004) were identified as independent risk factors for AEs. Survival analysis revealed that overall survival and progression-free survival (PFS) rates after nivolumab treatment differed significantly between the high and low groups of ALC, NLR, PLR, MLR and CAR. The multivariate analysis identified a low ALC [hazard ratio (HR): 3.710, P=0.003] and high CAR (HR: 2.953, P=0.007) as independent poor prognostic factors of PFS. In conclusion, ALC and CAR have potential as biomarkers for outcomes of recurrent esophageal cancer following nivolumab treatment.
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- 2022
125. Logical Communication Model and Real-Time Data Transmission Protocols for Embedded Systems with Controller Area Network.
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Kenya Sato and Hiroyuki Inoue
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- 2005
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126. Polymer heat-proofing using defibered plants obtained by wet-type bead milling of Japanese cedar
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Yuichiro Otsuka, Kazuhiro Shikinaka, Yoichi Tominaga, Hiroyuki Inoue, and Ai Tsukidate
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chemistry.chemical_classification ,Bead (woodworking) ,chemistry.chemical_compound ,Materials science ,Polymers and Plastics ,chemistry ,Chemical engineering ,Biological reaction ,Materials Chemistry ,food and beverages ,Polymer ,Ethylene carbonate - Abstract
In this Note, the heat-proofing and anti-plasticizing nature of poly(ethylene carbonate) by the addition of a defibered plant (DP) is presented. The DP was obtained via simple wet-milling treatment of water-dispersed Japanese cedar. The presented results encourage us to use plants as functional fillers without a special chemical/biological reaction. In this Note, the heat-proofing and anti-plasticizing nature of poly(ethylene carbonate) by the addition of a defibered plant (DP) is presented. The DP was obtained via simple wet-milling treatment for water-dispersed Japanese cedar. The presented results encourage us to use plants as functional fillers without a special chemical/biological reaction.
- Published
- 2021
127. Emotional Expressions of Real Humanoid Robots and Their Influence on Human Decision-Making in a Finite Iterated Prisoner’s Dilemma Game
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Hiroyuki Inoue, Yuki Kayukawa, Kazunori Terada, and Yasutake Takahashi
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0209 industrial biotechnology ,genetic structures ,General Computer Science ,Social Psychology ,Computer science ,02 engineering and technology ,Affect (psychology) ,Motion (physics) ,020901 industrial engineering & automation ,0501 psychology and cognitive sciences ,Emotional expression ,Electrical and Electronic Engineering ,050107 human factors ,Facial expression ,business.industry ,05 social sciences ,Robotics ,Prisoner's dilemma ,Human-Computer Interaction ,Philosophy ,Control and Systems Engineering ,Robot ,Artificial intelligence ,business ,human activities ,Humanoid robot ,Cognitive psychology - Abstract
Emotional expressiveness in robots has attracted increased research interest, and development of this technology has progressed in recent years. A wide variety of methods employing facial expressions, speech, body movements, and colors indicating emotional expression have been proposed. Although previous studies explored how emotional expressions are recognized and affect human behavior, cooperative and competitive human-robot relationships have not been well studied. In some cases, researchers have examined how cooperative relationships can be influenced by agents that exhibit detailed facial expressions on a screen. It remains unclear whether and how expressive whole-body movements of real humanoid robots influence cooperative decision-making. To explore this, we conducted an experiment in which participants played a finite iterated prisoner’s dilemma game with a small humanoid robot that exhibits multimodal emotional expressions through limb motions, LED lights, and speech. Results showed that participants were more cooperative when the humanoid robot showed emotional expression. This implies that real humanoid robots that lack ability to show sophisticated facial expressions can form cooperative relationships with humans by using whole-body motion, colors, and speech.
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- 2021
128. Pathophysiologic Mechanisms of Hypothermia-Induced Pancreatic Injury in a Rat Model of Body Surface Cooling
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Yoichi Katayama, Wakiko Aisaka, Eichi Narimatsu, Kazuhito Nomura, Shuji Uemura, Keisuke Harada, Hiroyuki Inoue, and Naofumi Bunya
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Male ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,H&E stain ,Apoptosis ,Rats, Sprague-Dawley ,Endocrinology ,Hypothermia, Induced ,Internal medicine ,Edema ,Internal Medicine ,medicine ,Animals ,Lactic Acid ,Interleukin 6 ,Pancreas ,Hepatology ,biology ,business.industry ,Pancreatic Diseases ,Hypothermia ,medicine.disease ,Pathophysiology ,Disease Models, Animal ,medicine.anatomical_structure ,Amylases ,Circulatory system ,biology.protein ,Cytokines ,Inflammation Mediators ,Pancreatic injury ,medicine.symptom ,business ,Biomarkers - Abstract
OBJECTIVE The mechanisms underlying hypothermia-induced pancreatic injury are unclear. Thus, we investigated the pathophysiology of hypothermia-induced pancreatic injury. METHODS We created a normal circulatory model with body surface cooling in rats. We divided the rats into control (36°C-38°C), mild hypothermia (33°C-35°C), moderate hypothermia (30°C-32°C), and severe hypothermia (27°C-29°C) (n = 5 per group) groups. Then, we induced circulatory failure with a cooling model using high-dose inhalation anesthesia and divided the rats into control (36°C-38°C) and severe hypothermia (27°C-29°C) (n = 5 per group) groups. Serum samples were collected before the introduction of hypothermia. Serum and pancreatic tissue were collected after maintaining the target body temperature for 1 hour. RESULTS Hematoxylin and eosin staining of the pancreas revealed vacuoles and edema in the hypothermia group. Serum amylase (P = 0.056), lactic acid (P < 0.05), interleukin 1β (P < 0.05), interleukin 6 (P < 0.05), and tumor necrosis factor α (P = 0.13) levels were suppressed by hypothermia. The circulatory failure model exhibited pancreatic injury. CONCLUSIONS Hypothermia induced bilateral effects on the pancreas. Morphologically, hypothermia induced pancreatic injury based on characteristic pathology typified by vacuoles. Serologically, hypothermia induced protective effects on the pancreas by suppressing amylase and inflammatory cytokine levels.
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- 2021
129. 7. Data Management in Radiation Therapy
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Yoshiki Takei and Hiroyuki Inoue
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Radiation therapy ,medicine.medical_specialty ,Text mining ,Computer science ,business.industry ,Data management ,medicine.medical_treatment ,medicine ,Medical physics ,General Medicine ,business ,Data Management - Published
- 2021
130. Development and demonstration of cannabis DNA detection kit using DNA chromatography chip
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Shuntaro Matsuta, Kenji Tsujikawa, Tatsuyuki Kanamori, Naoki Tachiiri, Hiroki Segawa, Miyamoto Shigehiko, Kenji Kuwayama, Yuko T. Iwata, Tadashi Yamamuro, Hiroyuki Inoue, and Ayumu Ishii
- Subjects
Dna detection ,chemistry.chemical_compound ,Chromatography ,biology ,Chemistry ,Cannabis ,Chip ,biology.organism_classification ,DNA - Published
- 2021
131. Histone deacetylase 6 regulates insulin signaling in pancreatic β cells
- Author
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Asuka Imai, Shun-ichiro Asahara, Chisako Hara, Yukina Kawada, Ayumi Kanno, Hiroyuki Inoue, Yoshiaki Kido, Maki Kimura-Koyanagi, and Yumiko Sugiura
- Subjects
Male ,0301 basic medicine ,Biophysics ,Type 2 diabetes ,Histone Deacetylase 6 ,Biochemistry ,Pancreatic beta cells ,Cell Line ,03 medical and health sciences ,0302 clinical medicine ,Insulin-Secreting Cells ,Type 2 diabetes mellitus ,medicine ,Animals ,Insulin ,Histone deacetylase ,Molecular Biology ,biology ,Chemistry ,Pancreatic islets ,Type 2 Diabetes Mellitus ,Cell Biology ,HDAC6 ,medicine.disease ,Mice, Inbred C57BL ,Insulin signaling ,Disease Models, Animal ,Insulin receptor ,030104 developmental biology ,Histone ,medicine.anatomical_structure ,Diabetes Mellitus, Type 2 ,Cell culture ,030220 oncology & carcinogenesis ,biology.protein ,Cancer research ,Signal Transduction - Abstract
The reduction of pancreatic β cell mass is one of the key factors for the onset of type 2 diabetes. Many reports have indicated that insulin signaling is important for type 2 diabetes, but the mechanism by which insulin signaling is altered in pancreatic β cells remains unclear. This study was designed to examine the role of histone deacetylases (HDACs) in the regulation of insulin signaling in pancreatic β cells. We found that insulin signaling was downregulated by inhibition of HDAC6. HDAC6 expression was specifically observed in pancreatic β cells and was decreased in the pancreatic islets of a type 2 diabetes mouse model. When a mouse pancreatic β cell line (MIN6 cells) was treated with palmitic acid to mimic the effect of a high-fat diet on pancreatic β cells, HDAC6 was imported into the nucleus. These results suggest that HDAC6 plays an important role in the regulation of insulin signaling in pancreatic β cells. Therefore, clarifying the regulation of insulin signaling by HDAC6 may be a valuable approach for the treatment of type 2 diabetes.
- Published
- 2021
132. Evaluation of test performance of Simon's reagent and Marquis reagent for so-called Ecstasy tablets
- Author
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Tatsuyuki Kanamori, Hiroki Segawa, Yuki Okada, Yuko T. Iwata, Kenji Tsujikawa, Kenji Kuwayama, Hiroyuki Inoue, and Tadashi Yamamuro
- Subjects
Chromatography ,Simon's reagent ,media_common.quotation_subject ,Reagent ,Ecstasy ,Test performance ,General Medicine ,Art ,media_common - Published
- 2021
133. Isolation, draft genome sequencing and identification of Enterobacter roggenkampii CCI9
- Author
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Hiroyuki Inoue, Noriyo Takeda, Yuya Itoiri, Zen-ichiro Kimura, Hironaga Akita, and Akinori Matsushika
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Genetics ,Whole genome sequencing ,Strain (chemistry) ,Sequence analysis ,Enterobacter ,average nucleotide identity ,Biology ,draft genome sequence ,rpoB ,Genome ,DNA sequencing ,multilocus sequence analysis ,16S rRNA ,Oligotroph ,Gene ,GC-content ,Research Paper - Abstract
Strain CCI9, which was isolated from leaf soil collected in Japan, was capable of growth on poor-nutrient medium, at temperatures of 10°C to 45°C, at pHs of 4.5 to 10, and in the presence of 7.0% NaCl. We determined a draft genome sequence of strain CCI9, which consists of a total of 28 contigs containing 4,644,734 bp with a GC content of 56.1%. This assembly yielded 4,154 predicted coding sequences. Multilocus sequence analysis (MLSA) based on atpD, gyrB, infB, and rpoB gene sequences were performed to further identify strain CCI9. The MLSA revealed that strain CCI9 clustered tightly with Enterobacter roggenkampii EN-117T. Moreover, the average nucleotide identity value (98.6%) between genome sequences of strain CCI9 and E. roggenkampii EN-117T exceeds the cutoff value for prokaryotic subspecies delineation. Therefore, strain CCI9 was identified as E. roggenkampii CCI9. To clarify differences between E. roggenkampii EN-117T and CCI9, the coding proteins were compared against the eggNOG database.
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- 2021
134. Methamphetamine-related forensic autopsy cases in a Japanese prefecture over a 7-year period: Characteristics of deaths and blood concentrations
- Author
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Go, Inokuchi, Fumiko, Chiba, Yumi, Hoshioka, Naoki, Saito, Maiko, Yoshida, Sayaka, Nagasawa, Yoshikazu, Yamagishi, Yohsuke, Makino, Suguru, Torimitsu, Rutsuko, Yamaguchi, Shigeki, Tsuneya, Hiroyuki, Inoue, Ayumi, Motomura, Daisuke, Yajima, and Hirotaro, Iwase
- Subjects
Issues, ethics and legal aspects ,Pathology and Forensic Medicine - Abstract
Understanding the actual conditions of methamphetamine (MA)-related death is important from the perspectives of criminal justice and public health. In this report, we review 104 cases of MA-related death handled by our departments between January 2014 and December 2020. Based on information from police and autopsy examinations, we classified the cases into the following categories: "accidental intoxication" ("MA only" and "multiple drugs or alcohol"), "fatal disease" ("definitively MA-related," "possibly MA-related," and "unlikely MA-related"), "accident," "suicide," "homicide," and "undetermined." The total number and annual trends for each category and their respective femoral blood concentrations were investigated. "Fatal disease" was the most common category (48 cases), followed by "suicide" (25 cases), "accidental intoxication" (14 cases), and "accident" (11 cases). "Definitively MA-related" in which MA may have played a role in their onset or exacerbation accounted for the majority of "fatal disease": 12 cases of heart disease, 4 cases of aortic dissection, 12 cases of cerebral hemorrhage, and 4 cases of subarachnoid hemorrhage. Cases classified as "definitively MA-related" died with lower femoral blood concentrations of MA compared with "MA only." Cases with "fatal disease" might have been misdiagnosed as "death by natural causes" if a proper autopsy and toxicology examinations were not performed. In death investigations, it is necessary to keep in mind that there are some MA-related deaths, and efforts should be made to increase awareness about the risk of death in using this drug.
- Published
- 2023
135. Automatic generation of fuzzy classification systems using hyper-cone membership functions.
- Author
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Lina Hng, Kenji Miyasaka, Hiroyuki Inoue, and Mitsuru Tsukamoto
- Published
- 2003
- Full Text
- View/download PDF
136. Difficulty in differentiating between IgG4-related hepatic inflammatory pseudotumor and intrahepatic cholangiocarcinoma
- Author
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Ai Hamano, Hiroyuki Inoue, Junya Tsuboi, Reiko Yamada, Yoshiyuki Takei, Noriyuki Horiki, Kyosuke Tanaka, and Kazunari Kurata
- Subjects
Male ,Hepatic inflammatory pseudotumor ,Pathology ,medicine.medical_specialty ,Cholangitis, Sclerosing ,Case Report ,Malignancy ,Granuloma, Plasma Cell ,Cholangiocarcinoma ,Diagnosis, Differential ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,parasitic diseases ,medicine ,Humans ,IgG4-related disease ,Intrahepatic Cholangiocarcinoma ,Aged ,Intrahepatic cholangiocarcinoma ,030203 arthritis & rheumatology ,Endoscopic retrograde cholangiopancreatography ,medicine.diagnostic_test ,Bile duct ,business.industry ,Gastroenterology ,General Medicine ,Liver biopsy ,Hepatology ,medicine.disease ,medicine.anatomical_structure ,Bile Duct Neoplasms ,Immunoglobulin G ,Inflammatory pseudotumor ,030211 gastroenterology & hepatology ,business - Abstract
A 71-year-old man on prednisolone for immunoglobulin (Ig) G4-related renal disease showed increased carbohydrate antigen (CA) 19–9 level; abdominal enhanced computed tomography (CT) showed a lesion in the left lateral segment and dilatation of the peripheral biliary duct. He was referred to our hospital for detailed examination for suspected intrahepatic cholangiocarcinoma. CT and magnetic resonance imaging findings were similar to those for intrahepatic cholangiocarcinoma. However, endoscopic retrograde cholangiopancreatography showed a smooth narrowing of the bile duct which suggested inflammatory disease. Liver biopsy was performed; IgG4-related hepatic inflammatory pseudotumor (IPT) was diagnosed. IgG4-related hepatic IPTs are rare diseases that develop in association with the development of sclerosing cholangitis. Most of these lesions develop in the hepatic hilum and the imaging findings of these tumors are similar to those of hilar cholangiocarcinomas. Thus, hepatic IPTs are difficult to differentiate from malignancy; in some cases, surgical resection has been considered for establishing the diagnosis. In the present case, we could diagnose hepatic IPT on the basis of liver biopsy, which is the recommended approach in cases of suspected hepatic IPT.
- Published
- 2020
137. Development of Core-Fucose-Deficient Humanized and Chimeric Anti-Human Podoplanin Antibodies
- Author
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Teizo Asano, Takuro Nakamura, Hideki Hosono, Manabu Kawada, Yusuke Sayama, Hiroyoshi Suzuki, Yukinari Kato, Tomokazu Ohishi, Junko Takei, Hiroyuki Inoue, Mika K. Kaneko, and Masato Sano
- Subjects
0301 basic medicine ,Platelet Aggregation ,medicine.drug_class ,government.form_of_government ,Immunology ,CHO Cells ,Monoclonal antibody ,Humanized antibody ,Fucose ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,Cricetulus ,0302 clinical medicine ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Immunology and Allergy ,Lectins, C-Type ,Neoplasm Metastasis ,PDPN ,Membrane Glycoproteins ,030102 biochemistry & molecular biology ,biology ,Chemistry ,Antibody-Dependent Cell Cytotoxicity ,Antibodies, Monoclonal ,Platelet Activation ,Antibodies, Neutralizing ,Xenograft Model Antitumor Assays ,Transmembrane protein ,Rats ,Lymphatic Endothelium ,Podoplanin ,Immunoglobulin G ,030220 oncology & carcinogenesis ,Cancer research ,biology.protein ,government ,Antibody ,Glioblastoma ,Protein Binding - Abstract
Podoplanin (PDPN), a 36-kDa type I transmembrane O-glycoprotein, is expressed in normal cells, including renal epithelial cells (podocytes), lymphatic endothelial cells, and pulmonary type I alveol...
- Published
- 2020
138. Association of Mps one binder kinase activator 1 (MOB1) expression with poor disease‐free survival in individuals with non‐small cell lung cancer
- Author
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Gouji Toyokawa, Kohei Otsubo, Yasuto Yoneshima, Nobuhisa Ando, Yoichi Nakanishi, Kentaro Tanaka, Yuki Ikematsu, Kayo Ijichi, Eiji Iwama, Maako Ide, Isamu Okamoto, Tetsuzo Tagawa, and Hiroyuki Inoue
- Subjects
0301 basic medicine ,Pulmonary and Respiratory Medicine ,Male ,Lung Neoplasms ,Hippo pathway ,lcsh:RC254-282 ,Disease-Free Survival ,03 medical and health sciences ,0302 clinical medicine ,Carcinoma, Non-Small-Cell Lung ,medicine ,Humans ,Risk factor ,Lung cancer ,vascular invasion ,Aged ,Retrospective Studies ,Hippo signaling pathway ,Gene knockdown ,Lung ,business.industry ,General Medicine ,Original Articles ,medicine.disease ,lung adenocarcinoma ,MOB1 ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,In vitro ,Chemokine CXCL10 ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,immunohistochemistry ,Cancer research ,Immunohistochemistry ,Adenocarcinoma ,Original Article ,Female ,business - Abstract
Background Mps one binder kinase activator 1 (MOB1) is a core component of the Hippo signaling pathway and has been implicated as a tumor suppressor. Here, we evaluated the possible relationship of MOB1 expression in non‐small cell lung cancer (NSCLC) to prognosis. Methods We retrospectively analyzed 205 lung adenocarcinoma patients treated at Kyushu University Hospital between November 2007 and October 2012. MOB1 expression in tumor cells of surgical specimens was evaluated by immunohistochemistry. Invasive activity of NSCLC cell lines in vitro was measured with a transwell assay. Results Expression of MOB1 was classified as high in 105 of the 205 (51.2%) tumor specimens, and such high expression was significantly associated with poor disease‐free survival (P = 0.0161). Among the various clinicopathologic parameters examined, high MOB1 expression was significantly associated only with intratumoral vascular invasion (P = 0.0005). Multivariate analysis also identified high MOB1 expression as a significant independent risk factor for disease‐free survival (P = 0.0319). The invasiveness of H1299 cells in vitro was increased or attenuated by overexpression or knockdown of MOB1, respectively. Conclusions Our results suggest that MOB1 might promote early recurrence of NSCLC by increasing vascular invasion by tumor cells. Key points Significant findings of the study We found that high MOB1 expression in surgical specimens of lung adenocarcinoma was associated with poor disease‐free survival and with intratumoral vascular invasion. MOB1 expression also promoted the invasiveness of NSCLC cells in vitro. What this study adds Our results thus suggest that high MOB1 expression is a risk factor for early postoperative recurrence in lung adenocarcinoma., We found that high MOB1 expression in surgical specimens of lung adenocarcinoma was associated with poor disease‐free survival and with intratumoral vascular invasion. MOB1 expression also promoted the invasiveness of NSCLC cells in vitro. Our results suggest that MOB1 might contribute to early recurrence in NSCLC.
- Published
- 2020
139. Fuzzy Classifier System Using Hyper-Cone Membership Functions and Rule Reduction Techniques.
- Author
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Hiroyuki Inoue, Keita Hatase, and Katsuari Kamei
- Published
- 2001
- Full Text
- View/download PDF
140. ASO Author Reflections: NADPH Oxidase 2 has Potential as a Therapeutic Target for Esophageal Squamous Cell Carcinoma
- Author
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Hiroki Shimizu, Keita Katsurahara, Hiroyuki Inoue, Atsushi Shiozaki, and Eigo Otsuji
- Subjects
Esophagectomy ,Esophageal Neoplasms ,Oncology ,NADPH Oxidase 2 ,Carcinoma, Squamous Cell ,Humans ,Surgery ,Esophageal Squamous Cell Carcinoma - Published
- 2022
141. Bioprotective Role of Platelet-Derived Microvesicles in Hypothermia: Insight into the Differential Characteristics of Peripheral and Splenic Platelets
- Author
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Kie Horioka, Hiroki Tanaka, Keisuke Okaba, Shinnosuke Yamada, Akira Hayakawa, Namiko Ishii, Ayumi Motomura, Hiroyuki Inoue, Shuhei Takauji, Shotaro Isozaki, Katsuhiro Ogawa, Daisuke Yajima, Henrik Druid, Lasse Pakanen, and Katja Porvari
- Subjects
History ,Polymers and Plastics ,Hematology ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2022
142. Development of Walking Assist Machine Using Linkage Mechanism - Mechanism and its Fundamental Motion -.
- Author
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Hiroyuki Inoue and Toshiro Noritsugu
- Published
- 2010
- Full Text
- View/download PDF
143. Fish assemblage structure response to seagrass bed degradation due to overgrazing by the green sea turtle Chelonia mydas at Iriomote Island, southern Japan
- Author
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Akira Mizutani, Kouki Tsutsumi, Hiroyoshi Kohno, Hiroyuki Inoue, and Kusuto Nanjo
- Subjects
0106 biological sciences ,Green sea turtle ,biology ,Ecology ,Rabbit fish ,04 agricultural and veterinary sciences ,Vegetation ,biology.organism_classification ,010603 evolutionary biology ,01 natural sciences ,Ostorhinchus ,Seagrass ,Abundance (ecology) ,Benthic zone ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,Overgrazing ,Ecology, Evolution, Behavior and Systematics - Abstract
The fish assemblage structure response to rapid degradation of Enhalus acoroides seagrass beds due to overgrazing by green sea turtles (Chelonia mydas) was investigated at Iriomote Island, southern Japan, by visual censusing of fish species in several microhabitats in and around the beds (i.e., dense seagrass bed, grazed bed, their boundaries, and adjacent sandy area). Fish assemblage structure differed among the seagrass microhabitats, both species and individual numbers being higher in microhabitats with seagrass compared to overgrazed beds and unvegetated sandy areas, together with different species composition. In the dense seagrass beds and boundary areas (the border area between dense seagrass beds and grazed areas), seagrass-associated fishes such as the rabbit fish Siganus fuscescens and cardinal fish Ostorhinchus ishigakiensis were abundant and comparable with those in other seagrass beds in the area. In addition, the fish assemblages in those microhabitats varied seasonally, fish abundance being greater in summer due to higher levels of recruitment. In the grazed bed, benthic gobies, such as Ctenogobiops crocineus and Cryptocentrus caeruleomaculatus, were dominant (similarly so in unvegetated sandy areas), and seasonal variations in such assemblages were relatively low compared to those in dense seagrass microhabitats. The decrease in the abundance and diversity of seagrass-associated fish in the grazed areas could be explained partly by the shortage of vegetation (
- Published
- 2020
144. Multi-step Simulation of Vacuum-carburizing Reactor Based on Kinetics Approach
- Author
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Masahide Inagaki, Soichiro Makino, Koji Tanaka, Koji Inagaki, Hiroyuki Inoue, and Hideaki Ikehata
- Subjects
Materials science ,Computer simulation ,business.industry ,Nuclear engineering ,Mechanical Engineering ,Kinetics ,Metals and Alloys ,Mechanics ,Computational fluid dynamics ,Condensed Matter Physics ,Carburizing ,chemistry.chemical_compound ,Acetylene ,chemistry ,Mechanics of Materials ,Materials Chemistry ,Physical and Theoretical Chemistry ,business - Published
- 2020
145. Gastropod shell use by the land hermit crabs Coenobita brevimanus and C. cavipes in an abandoned village on Iriomotejima Island, Japan
- Author
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Akira Mizutani, Hiroyoshi Kohno, Wataru Doi, and Hiroyuki Inoue
- Subjects
Coenobita brevimanus ,Geography ,biology ,Ecology ,General Medicine ,biology.organism_classification ,Gastropod shell - Published
- 2020
146. Structural Origin of Additional Infrared Transparency and Enhanced Glass-Forming Ability in Rare-Earth-Rich Borate Glasses without B–O Networks
- Author
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Koji Ohara, Atsunobu Masuno, Shunta Sasaki, Hiroyuki Inoue, Shinji Kohara, Yasuhiro Watanabe, and Yutaka Yanaba
- Subjects
Inorganic Chemistry ,Lanthanide ,Ionic radius ,Infrared ,Chemistry ,Phase (matter) ,Coordination number ,Analytical chemistry ,Thermal stability ,Crystal structure ,Physical and Theoretical Chemistry ,Glass transition - Abstract
R2O3-B2O3 binary glasses (R denotes rare-earth elements or Y) were fabricated in a very wide composition region using a levitation technique. The maximum R2O3 content of light rare-earth compounds reached 63 mol % and decreased with a decrease in the ionic radius of R3+. The thermal, optical, vibrational, and structural properties were investigated, particularly for 50R2O3-50B2O3 glasses. The glass transition temperature increased with a decrease in the ionic radius of R3+, while the thermal stability was not affected by the glass composition. The packing density increased with a decrease in the ionic radius of R3+ due to lanthanoid contraction. Raman scattering and Fourier transform infrared spectra revealed that, in the rare-earth-rich glasses, no conventional three-dimensional networks consisting of corner-sharing BOn (n = 3 or 4) units existed because all B atoms were formed as isolated BO3 units. The simple environment around B atoms in the glasses led to additional IR transmittance regions, irrespective of the kinds of R. The total correlation functions obtained from high-energy X-ray diffraction measurements were analyzed using the pair-function method and compared with those of various RBO3 crystalline phases. It was suggested that the local structure around R resembles the ν-NdBO3-type crystal structure, and the O coordination number of R ranged from 6.5 to 7.7, smaller than that of the crystalline phase. The glass-forming ability depending on R was discussed based on the structural similarities between the melt, glass, and crystalline phases.
- Published
- 2020
147. Crystal structure of GH30-7 endoxylanase C from the filamentous fungusTalaromyces cellulolyticus
- Author
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Yusuke Nakamichi, Masahiro Watanabe, Hiroyuki Inoue, Tatsuya Fujii, and Akinori Matsushika
- Subjects
Models, Molecular ,Protein Conformation, alpha-Helical ,Stereochemistry ,Genetic Vectors ,Mutant ,Biophysics ,Substituent ,Gene Expression ,Glucuronates ,Xylose ,Crystallography, X-Ray ,Biochemistry ,Substrate Specificity ,Research Communications ,Fungal Proteins ,03 medical and health sciences ,chemistry.chemical_compound ,Residue (chemistry) ,Structural Biology ,Catalytic Domain ,Arabinoxylan ,Escherichia coli ,Genetics ,Protein Interaction Domains and Motifs ,Glycoside hydrolase ,Cloning, Molecular ,Bifunctional ,030304 developmental biology ,0303 health sciences ,Endo-1,4-beta Xylanases ,biology ,030306 microbiology ,Active site ,Condensed Matter Physics ,Recombinant Proteins ,Amino Acid Substitution ,Carbohydrate Sequence ,Talaromyces ,chemistry ,biology.protein ,Protein Conformation, beta-Strand ,Xylans ,Protein Binding - Abstract
GH30-7 endoxylanase C from the cellulolytic fungusTalaromyces cellulolyticus(TcXyn30C) belongs to glycoside hydrolase family 30 subfamily 7, and specifically releases 22-(4-O-methyl-α-D-glucuronosyl)-xylobiose from glucuronoxylan, as well as various arabino-xylooligosaccharides from arabinoxylan.TcXyn30C has a modular structure consisting of a catalytic domain and a C-terminal cellulose-binding module 1 (CBM1). In this study, the crystal structure of aTcXyn30C mutant which lacks the CBM1 domain was determined at 1.65 Å resolution. The structure of the active site ofTcXyn30C was compared with that of the bifunctional GH30-7 xylanase B fromT. cellulolyticus(TcXyn30B), which exhibits glucuronoxylanase and xylobiohydrolase activities. The results revealed thatTcXyn30C has a conserved structural feature for recognizing the 4-O-methyl-α-D-glucuronic acid (MeGlcA) substituent in subsite −2b. Additionally, the results demonstrated that Phe47 contributes significantly to catalysis byTcXyn30C. Phe47 is located in subsite −2b and also near the C-3 hydroxyl group of a xylose residue in subsite −2a. Substitution of Phe47 with an arginine residue caused a remarkable decrease in the catalytic efficiency towards arabinoxylan, suggesting the importance of Phe47 in arabinoxylan hydrolysis. These findings indicate that subsite −2b ofTcXyn30C has unique structural features that interact with arabinofuranose and MeGlcA substituents.
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- 2020
148. Association between mean platelet volume in the pathogenesis of type 2 diabetes mellitus and diabetic macrovascular complications in Japanese patients
- Author
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Shun-ichiro Asahara, Hiroyuki Inoue, Kumiko Kouchi, Mayumi Saito, Yoshiaki Kido, and Fumihiko Nakamura
- Subjects
Male ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Mean platelet volume ,Type 2 diabetes ,Gastroenterology ,Diseases of the endocrine glands. Clinical endocrinology ,Prediabetic State ,chemistry.chemical_compound ,Vascular Stiffness ,Japan ,Diabetes mellitus ,Internal medicine ,Internal Medicine ,medicine ,Humans ,Prediabetes ,Aged ,Glycated Hemoglobin ,business.industry ,Confounding ,Type 2 Diabetes Mellitus ,Articles ,General Medicine ,Atherosclerosis ,medicine.disease ,RC648-665 ,Arterial stiffness ,Clinical Science and Care ,Diabetes Mellitus, Type 2 ,chemistry ,Multivariate Analysis ,Disease Progression ,Linear Models ,Female ,Original Article ,Glycated hemoglobin ,business ,Biomarkers ,Diabetic Angiopathies - Abstract
Aims/Introduction Mean platelet volume (MPV) is a widely used biological marker of platelet function and activity. Increased MPV is associated with accelerated thrombopoiesis and an elevated risk of cardiovascular disease. However, it is not known whether higher MPV is related to the pathogenesis of type 2 diabetes and diabetic macrovascular complications in Japanese patients. Therefore, we analyzed MPV and its correlation with atherosclerosis in Japanese patients with type 2 diabetes and those who had prediabetes. Materials and Methods We divided the patients into three groups: normoglycemic patients (n = 56), prediabetes patients (n = 44) and type 2 diabetes patients group, (n = 115). We measured platelet parameters and evaluated arterial stiffness in the three groups. Results Significantly higher MPV was found in the type 2 diabetes mellitus and prediabetes patients compared with normoglycemic patients. MPV was significantly correlated with fasting blood glucose and glycated hemoglobin levels. Multiple linear regression analysis showed that MPV was positively correlated with HbA1c, even after adjustment for confounding factors. In the evaluation of arterial stiffness by measuring the cardio‐ankle vascular index and maximum intima‐media thickness, MPV showed a positive correlation with these parameters. Conclusions These findings suggest that MPV was significantly increased in the early stage of type 2 diabetes. We showed positive correlations between MPV and HbA1c levels, and between MPV and arterial stiffness in Japanese patients with type 2 diabetes., In Japanese patients with type 2 diabetes, mean platelet volume was significantly increased. Increased mean platelet volume led to progression of atherosclerosis. It was suggested that mean platelet volume was positively associated with the pathogenesis of type 2 diabetes mellitus in Japanese patients.
- Published
- 2020
149. Paired genetic analysis by next‐generation sequencing of lung cancer and associated idiopathic pulmonary fibrosis
- Author
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Atsushi Osoegawa, Kohei Otsubo, Kentaro Tanaka, Yasuto Yoneshima, Yoichi Nakanishi, Eiji Iwama, Hiroyuki Inoue, Isamu Okamoto, Naoki Kubo, Tetsuzo Tagawa, and Kayo Ijichi
- Subjects
Male ,0301 basic medicine ,Cancer Research ,Pathology ,Lung Neoplasms ,Somatic cell ,next‐generation sequencing ,medicine.disease_cause ,Genetic analysis ,Pathogenesis ,Idiopathic pulmonary fibrosis ,0302 clinical medicine ,ERBB3 ,High-Throughput Nucleotide Sequencing ,General Medicine ,Middle Aged ,respiratory system ,idiopathic pulmonary fibrosis ,humanities ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,Original Article ,Female ,Adult ,medicine.medical_specialty ,03 medical and health sciences ,Clinical Research ,medicine ,Humans ,Genetic Predisposition to Disease ,Genetic Testing ,Lung cancer ,Genetic Association Studies ,Aged ,Neoplasm Staging ,Lung ,business.industry ,Genetic Variation ,Original Articles ,cancer‐related gene ,Sequence Analysis, DNA ,medicine.disease ,respiratory tract diseases ,lung cancer ,030104 developmental biology ,business ,Carcinogenesis ,Biomarkers ,somatic alteration - Abstract
The pathogenesis of lung cancer associated with idiopathic pulmonary fibrosis (IPF) has remained largely uncharacterized. To provide insight into this condition, we undertook genomic profiling of IPF‐associated lung cancer as well as of adjacent fibrosing lung tissue in surgical specimens. Isolated DNA and RNA from 17 IPF‐associated non‐small cell lung cancer and 15 paired fibrosing lung tissue specimens were analyzed by next‐generation sequencing with a panel that targets 161 cancer‐related genes. Somatic genetic alterations were frequently identified in TP53 (n = 6, 35.3%) and PIK3CA (n = 5, 29.4%) genes in tumor samples as well as in EGFR (n = 7, 46.7%), PIK3CA (n = 5, 33.3%), ERBB3 (n = 4, 26.7%), and KDR (n = 4, 26.7%) in IPF samples. Genes related to the RAS‐RAF signaling pathway were also frequently altered in tumor (n = 7, 41.2%) and IPF (n = 3, 20.0%) samples. The number of somatic alterations identified in IPF samples was almost as large as that detected in paired tumor samples (81 vs 90, respectively). However, only 6 of the 81 somatic alterations detected in IPF samples overlapped with those in paired tumor samples. The accumulation of somatic mutations was thus apparent in IPF tissue of patients with IPF‐associated lung cancer, and the RAS‐RAF pathway was implicated in lung tumorigenesis. The finding that somatic alterations were not frequently shared between tumor and corresponding IPF tissue indicates that IPF‐associated lung cancer does not develop through the stepwise accumulation of somatic alterations in IPF., The genetic basis of idiopathic pulmonary fibrosis‐associated lung cancer remains largely unknown. We show that somatic alterations were frequently identified in fibrosing lung tissue as much as that in tumor tissue, and that genes related to the RAS‐RAF signaling pathway were frequently altered in both specimens. These findings can provide a basis for the development of targeted drugs for such tumors.
- Published
- 2020
150. Investigation of the Atmospheric Corrosion in the Factory Site of a Littoral Industrial Complex Using Carbon Steel Adhesive-backed Test Coupons
- Author
-
Kazuo Komori, Ryo Asakura, Hiroyuki Muramatsu, Masashi Ono, and Hiroyuki Inoue
- Subjects
Carbon steel ,Atmospheric corrosion ,Metallurgy ,Materials Chemistry ,Electrochemistry ,Metals and Alloys ,engineering ,Littoral zone ,Environmental science ,Factory ,Adhesive ,engineering.material ,Surfaces, Coatings and Films - Published
- 2020
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