715 results on '"D. Pillay"'
Search Results
102. Respiratory virus infections in immunocompromised patients
- Author
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D. Pillay and D. Muir
- Subjects
Microbiology (medical) ,Paramyxoviridae Infections ,business.industry ,Adenoviridae Infections ,General Medicine ,Respiratory Syncytial Virus Infections ,Microbiology ,Virology ,Immunocompromised Host ,Influenza, Human ,Respiratory virus ,Medicine ,Humans ,business ,Respiratory Tract Infections - Published
- 1998
103. GBV-C/HGV coinfection in HIV-1-positive men: frequent detection of viral RNA in blood plasma but absence from seminal fluid plasma
- Author
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R C, Hollingsworth, C L, Jameson, J E, Minton, M, Crowe, R, Curran, T, Rowe, A M, Grabowska, D, Pillay, W L, Irving, and J K, Ball
- Subjects
Male ,Hepatitis, Viral, Human ,Reverse Transcriptase Polymerase Chain Reaction ,Flaviviridae ,HIV Infections ,Hepacivirus ,Hepatitis C ,CD4 Lymphocyte Count ,Cohort Studies ,Proviruses ,Semen ,DNA, Viral ,HIV-1 ,Prevalence ,Humans ,RNA, Viral ,Homosexuality, Male - Abstract
Sequential paired samples of blood and seminal fluid were obtained from a cohort of 54 HIV-1-infected homosexual males. The prevalence of GBV-C/HGV RNA in the cell-free fractions of some of these patients was determined using reverse-transcription polymerase chain reaction (RT-PCR). To assess the effects of HIV-1 and HCV infection upon GBV-C/HGV RNA status, blood CD4 cell counts, HCV RNA status, and HIV-1 proviral DNA and viral RNA titres were also determined. GBV-C/HGV RNA was detected in 8/30 (27%) of the blood plasma samples obtained at the start of the study, and was present at a frequency of 14/64 (22%) in all the blood plasma samples tested. By contrast, GBV-C/HGV RNA was not detected in the 26 seminal fluid samples obtained at the start of the study, including 8 samples obtained from patients for which GBV-C/HGV RNA was detected in the corresponding blood sample. Of the samples tested for the presence of both GBV-C/HGV and HCV RNA, there was no evidence of coinfection. Although GBV-C/HGV RNA detection rates were significantly higher in individuals with blood CD4 cell counts greater than 200 cells per microlitre, there were no significant differences in the median blood CD4 cell counts or HIV-1 proviral DNA or viral RNA titres observed between the GBV-C/HGV-positive and -negative individuals. The failure to detect GBV-C/HGV RNA in seminal fluid samples obtained from this cohort would suggest that further studies need to be carried out to determine the roles of sexual transmission and of seminal fluid in GBV-C/HGV infection.
- Published
- 1998
104. Emergence and control of resistance to antiviral drugs in resistance in herpes viruses, hepatitis B virus, and HIV
- Author
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D, Pillay
- Subjects
Hepatitis B virus ,HIV ,Humans ,Drug Resistance, Microbial ,Microbial Sensitivity Tests ,Antiviral Agents ,Herpesviridae - Abstract
This article describes the biology of antiviral drug resistance with regard to herpes viruses, hepatitis B virus, and HIV. The emergence of antiviral drug resistance is testimony to the potency of current antiviral drugs. Natural selection of such viral variants occurs, such that the strains that are 'fittest' in the presence of the drug persist. Phenotypic and genotypic assays are now available to test clinical viral isolates for resistance. At present, the clearest indication for such testing is in aciclovir-resistant herpes simplex infection. It is important that other resistance assays are subject to studies of clinical utility before their widespread use.
- Published
- 1998
105. Audit of HIV RNA quantitation measurements in the West Midlands: a preliminary report. Collaborating HIV Physicians
- Author
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M E, Ashcroft, S J, Skidmore, B, Olowokure, and D, Pillay
- Subjects
Adult ,Male ,Medical Audit ,HIV ,Humans ,RNA, Viral ,Female ,HIV Infections ,Viral Load ,United Kingdom - Published
- 1998
106. Urinary levels of tissue kallikrein in black and Indian hypertensives and their implications for therapy
- Author
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Y K, Seedat, K D, Bhoola, R, Muller, I G, Randeree, and D, Pillay
- Subjects
Adult ,Male ,Analysis of Variance ,Time Factors ,Black People ,India ,Blood Pressure ,Middle Aged ,Statistics, Nonparametric ,White People ,Electrolytes ,Hypertension ,Humans ,Female ,Kallikreins - Abstract
It is accepted that blacks differ from white and Indian hypertensives in their response to hypotensive agents. Black hypertensives in the USA have lower urinary tissue kallikrein (TK) excretion levels than white hypertensives. It has been suggested that blacks respond better to thiazide diuretics than to beta-blockers because thiazides increase TK excretion whereas beta-blockers decrease it. This study compares the excretion of urinary TK in black and Indian hypertensive and normotensive subjects. Urinary TK levels were measured with the selective, synthetic peptic substrate with the sequence of H-D-Val-Leu-Arg-pNA. Ten hypertensive patients on placebo therapy and 10 normotensive black and Indian subjects provided 3 samples at weeks 0, 2 and 4 for the determination of urinary TK. The results were analysed and analysis of variance was used to compare the two racial groups. There were no significant differences in urinary TK values of the three biweekly individual samples. Urinary TK values (ng TK/microgram protein) in Indian hypertensives were generally lower than in black hypertensives.
- Published
- 1998
107. High prevalence of hepatitis G virus in bone marrow transplant recipients and patients treated for acute leukemia
- Author
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S J, Skidmore, K E, Collingham, P, Harrison, J R, Neilson, D, Pillay, and D W, Milligan
- Subjects
Adult ,Leukemia ,Hepatitis, Viral, Human ,Flaviviridae ,Transfusion Reaction ,Blood Donors ,Hepatitis C ,Tissue Donors ,United Kingdom ,Hematologic Neoplasms ,Acute Disease ,Antineoplastic Combined Chemotherapy Protocols ,Prevalence ,Humans ,Mass Screening ,RNA, Viral ,Blood Transfusion ,Aspartate Aminotransferases ,Biomarkers ,Bone Marrow Transplantation ,Retrospective Studies - Abstract
Hepatitis G virus (HGV) is a newly described virus that has been implicated in transfusion-associated hepatitis. The prevalence of HGV in a group of multitransfused patients with hematological malignancy was studied using a reverse transcription polymerase chain reaction technique. Transfusion histories and serum aspartate aminotransferase (AST) levels were recorded. HGV was detected in 29 of 60 (48%) patients. There was no difference in HGV positivity rates between those with normal AST levels and those with raised AST levels. Analysis of patients by treatment type showed that 20 of 33 (61%) patients who received a bone marrow transplantation procedure were HGV positive compared with 9 of 27 (33%) treated with conventional combination chemotherapy (P = .036) despite similar transfusion histories. There was no significant difference in HGV positivity between patients treated before the introduction of United Kingdom blood donor screening for hepatitis C virus antibody:18 of 39 (46%) and those treated after the introduction of screening 11 of 21 (52%). HGV infection appears to be extremely common in these patients; however, the clinical significance of these findings with respect to liver dysfunction is not yet clear.
- Published
- 1997
108. Zinc status in vitamin B6 deficiency
- Author
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D, Pillay, P, Gathiram, and J B, Ubbink
- Subjects
Male ,Eating ,Zinc ,Erythrocytes ,Pyridoxal Phosphate ,Animals ,Nutritional Status ,Metallothionein ,Rats, Wistar ,Vitamin B 6 Deficiency ,Weight Gain ,Rats - Abstract
Three groups of young male Wistar rats were maintained on diets consisting of 7 mg pyridoxine hydrochloride/kg diet (control and pair-fed groups) and 0 mg pyridoxine hydrochloride/ kg diet (deficient group) for six weeks. The zinc status of all rats was assessed by measuring their erythrocyte zinc-metallothionein-1 (Zn-Mt-1) and plasma zinc levels. A significant difference (p0.001) in plasma zinc levels was observed between the deficient group and the control and pair-fed groups (1.35 micrograms/ml +/- 0.08, 1.99 micrograms/ml +/- 0.06 and 2.03 micrograms/ml +/- 0.07 respectively). Erythrocyte Zn-Mt-1 levels were significantly lower in vitamin B6 deficient rats when compared to control animals. No significant difference in Zn-Mt-1 levels existed between vitamin B6 deficient and pair-fed groups suggesting that the reduced Zn-Mt-1 levels in vitamin B6 deficient rats may be due entirely to their decreased food intake (8.9 g/day compared to 15 g/day of control rats).
- Published
- 1997
109. Random-amplified polymorphic DNA (RAPD) analysis shows intraspecies differences among Xanthomonas albilineans strains
- Author
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K. Permaul, D. Pillay, and B. Pillay
- Subjects
Genetics ,DNA, Bacterial ,Xanthomonas ,Biology ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Molecular biology ,DNA Fingerprinting ,RAPD ,law.invention ,Random Amplified Polymorphic DNA Technique ,genomic DNA ,DNA profiling ,Genetic marker ,law ,Xanthomonas albilineans ,Agarose gel electrophoresis ,Polymerase chain reaction - Abstract
Five strains of Xanthomonas albilineans, causal agent of leaf scald disease in sugarcane from various geographical regions, were compared using random amplification of polymorphic DNA (RAPD) to determine whether they could be differentiated at the DNA level. CsCl-purified genomic DNA from these strains were amplified by the polymerase chain reaction (PCR) using arbitrary 10-mer primers according to standard RAPD conditions and the amplification product profiles analysed by conventional agarose gel electrophoresis. Although most RAPD markers were common to all five strains, unique profiles for each strain were discernible using four 10-mer arbitrary primers individually. Reproducible DNA fingerprints indicate that RAPD analysis can be used to identify and differentiate the X. albilineans strains. This technique has the potential for use in monitoring the appearance of foreign strains of X. albilineans in various geographical regions and could be used for the construction of phylogenetic trees.
- Published
- 1996
110. Antimicrobial therapy: toward the future
- Author
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R, Ellis and D, Pillay
- Subjects
Drug Design ,Research ,Antiprotozoal Agents ,Drug Resistance ,Humans ,Antiviral Agents ,Drug Approval ,Anti-Bacterial Agents ,Forecasting - Abstract
The relatively unsophisticated approaches at the beginnings of antimicrobial therapy development, although important at the time, have been replaced by ever more complex and ingenious methods as the medical profession and the pharmaceutical industries face up to the realities of the emerging problems of antimicrobial resistance.
- Published
- 1996
111. Transacting Functions of Human Retroviruses: Current Topics in Microbiology and Immunology
- Author
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D Pillay
- Subjects
business.industry ,Book Reviews ,Medicine ,Computational biology ,Current (fluid) ,business ,Bioinformatics ,Pathology and Forensic Medicine - Published
- 1996
112. Maternal infections. Part 2: Cytomegalovirus
- Author
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D, Pillay
- Subjects
Infection Control ,Neonatal Screening ,Risk Factors ,Cytomegalovirus Infections ,Infant, Newborn ,Humans ,Infectious Disease Transmission, Vertical - Published
- 1996
113. Body Mass Index and Self-Perception of Overweight and Obesity in Rural, Urban and Rural-to-Urban Migrants: PERU MIGRANT Study
- Author
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Timesh D Pillay, Liam Smeeth, Robert H. Gilman, Christian Loret de Mola, Francisco Diez-Canseco, and J. Jaime Miranda
- Subjects
Male ,Rural Population ,Gerontology ,demography ,Urban Population ,Epidemiology ,Cross-sectional study ,health status ,Overweight ,Global Health ,Social and Behavioral Sciences ,migration ,Cultural Anthropology ,Body Mass Index ,0302 clinical medicine ,Cohen's kappa ,Peru ,lowest income group ,030212 general & internal medicine ,Transients and Migrants ,2. Zero hunger ,Multidisciplinary ,Anthropometry ,article ,1. No poverty ,Middle Aged ,waist circumference ,Medicine ,Female ,disease severity ,medicine.symptom ,Attitude to Health ,Research Article ,Adult ,Waist ,Science ,Human Migration ,self report ,urbanization ,030209 endocrinology & metabolism ,urban rural difference ,Biology ,body weight ,03 medical and health sciences ,attitude to health ,medicine ,Humans ,Obesity ,Geographic and National Differences ,Poverty ,Cardiovascular Disease Epidemiology ,Aged ,Nutrition ,Models, Statistical ,statistical model ,nutritional and metabolic diseases ,medicine.disease ,major clinical study ,Cross-Sectional Studies ,self concept ,Social Class ,Anthropology ,purl.org/pe-repo/ocde/ford#3.01.00 [https] ,Perception ,Rural area ,Body mass index ,Demography - Abstract
ObjectiveThis study aimed to compare self-reported weight and body mass index (BMI) in order to determine discrepancies between subjective and objective obesity-related markers, and possible explanatory factors of overweight and obesity underestimation, in urban, rural and migrant populations.Materials and methodsData from the PERU MIGRANT study, a cross-sectional study, in low-income settings, of urban, migrant (rural-to-urban), and rural groups, including BMI, self-reported weight and socio-demographic indicators were analyzed. Percentage of concurrences between BMI and self-reported weight and Kappa coefficients for inter-rater agreement were calculated. Univariate and standardized descriptive analyses were performed to identify potential explanatory variables for weight underestimation in only overweight and obese individuals, using established BMI and waist circumference cut offs.Results983 Participants-199 urban, 583 migrants and 201 rural-were analyzed. Based on BMI, overall prevalence of obesity was 20.1% (95% CI 17.6%-22.6%), and overweight was 38.3% (95% CI 35.2%-41.2%), with differences between study groups (pDiscussionOverall, high levels of overweight, obesity, and underestimation of BMI status were found, with poor agreement between BMI and self-reported weight, showing the unawareness of weight status severity in this low-income setting.
- Published
- 2012
114. HIV infection in a long-term renal transplant recipient
- Author
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Margaret Johnson, S. B. Squire, P. Sweny, D. Pillay, and Marc Lipman
- Subjects
Adult ,Male ,medicine.medical_specialty ,Time Factors ,HIV Infections ,Dermatology ,HIV Antibodies ,Nephropathy ,Fatal Outcome ,Postoperative Complications ,Acquired immunodeficiency syndrome (AIDS) ,Internal medicine ,Immunopathology ,medicine ,Humans ,Pharmacology (medical) ,Sida ,Kidney transplantation ,Kidney ,biology ,business.industry ,Public Health, Environmental and Occupational Health ,medicine.disease ,biology.organism_classification ,Kidney Transplantation ,Transplantation ,Infectious Diseases ,medicine.anatomical_structure ,Immunology ,Cytomegalovirus Infections ,HIV-1 ,Viral disease ,business - Published
- 1995
115. Working on wellness (WOW): A worksite health promotion intervention programme
- Author
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Estelle V. Lambert, Willem van Mechelen, Tracy Kolbe-Alexander, Leegale Adonis, Julian D Pillay, Marieke F van Wier, Craig Nossel, Karin I. Proper, Health Economics and Health Technology Assessment, Methodology and Applied Biostatistics, EMGO+ - Quality of Care, MRC/UCT RU for Exercise and Sport Medicine, Faculty of Health Sciences, Public and occupational health, Epidemiology and Data Science, and EMGO - Quality of care
- Subjects
Program evaluation ,Gerontology ,Adult ,Male ,cholesterol concentrations ,Motivational interviewing ,Occupational Health Services ,Pilot Projects ,Health Promotion ,Motivational Interviewing ,elevated blood pressure ,Risk Assessment ,law.invention ,Intervention mapping ,Study Protocol ,Nursing ,Randomized controlled trial ,law ,cardiovascular disease ,Risk Factors ,Intervention (counseling) ,Medicine ,overweight ,Humans ,Program Development ,business.industry ,lcsh:Public aspects of medicine ,Public Health, Environmental and Occupational Health ,lcsh:RA1-1270 ,insufficient fruit and vegetable intake ,Middle Aged ,Health promotion ,Cardiovascular Diseases ,Absenteeism ,Female ,Risk assessment ,business ,Working on Wellness (WOW) ,Follow-Up Studies ,Program Evaluation - Abstract
Background Insufficient PA has been shown to cluster with other CVD risk factors including insufficient fruit and vegetable intake, overweight, increased serum cholesterol concentrations and elevated blood pressure. This paper describes the development of Working on Wellness (WOW), a worksite intervention program incorporating motivational interviewing by wellness specialists, targeting employees at risk. In addition, we describe the evaluation the effectiveness of the intervention among employees at increased risk for cardiovascular disease. Methods The intervention mapping (IM) protocol was used in the planning and design of WOW. Focus group discussions and interviews with employees and managers identified the importance of addressing risk factors for CVD at the worksite. Based on the employees’ preference for individual counselling, and previous evidence of the effectiveness of this approach in the worksite setting, we decided to use motivational interviewing as part of the intervention strategy. Thus, as a cluster-randomised, controlled control trial, employees at increased risk for CVD (N = 928) will be assigned to a control or an intervention group, based on company random allocation. The sessions will include motivational interviewing techniques, comprised of two face-to-face and four telephonic sessions, with the primary aim to increase habitual levels of PA. Measures will take place at baseline, 6 and 12 months. Secondary outcomes include changes in nutritional habits, serum cholesterol and glucose concentrations, blood pressure and BMI. In addition, healthcare expenditure and absenteeism will be measured for the economic evaluation. Analysis of variance will be performed to determine whether there were significant changes in physical activity habits in the intervention and control groups at 6 and 12 months. Discussion The formative work on which this intervention is based suggests that the strategy of targeting employees at increased risk for CVD is preferred. Importantly, this study extends the work of a previous, similar study, Health Under Construction, in a different setting. Finally, this study will allow an economic evaluation of the intervention that will be an important outcome for health care funders, who ultimately will be responsible for implementation of such an intervention. Trial registration United States Clinical Trails Register NCT 01494207
- Published
- 2012
116. Value of routine surveillance cultures for detection of CMV pneumonitis following bone marrow transplantation
- Author
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A, Webster, B, Blizzard, D, Pillay, H G, Prentice, K, Pothecary, and P D, Griffiths
- Subjects
Predictive Value of Tests ,Risk Factors ,Cytomegalovirus Infections ,Pneumonia, Viral ,Radioimmunoassay ,Cytomegalovirus ,Humans ,Viremia ,Antibodies, Viral ,Saliva ,Lung ,Cells, Cultured ,Bone Marrow Transplantation - Abstract
The results of 5018 surveillance cultures for the detection of cytomegalovirus (CMV) from 177 patients undergoing allogeneic T cell-depleted bone marrow transplantation (BMT) were analysed to determine their value in predicting future development of CMV pneumonitis. Twenty-two patients were diagnosed as having CMV pneumonitis. The median times of a positive CMV culture result from urine, saliva or blood before disease in these patients were 32, 8 and 23 days, respectively. The positive predictive value of CMV viraemia for the development of CMV pneumonitis was 0.64. Multivariate analysis showed that recipient pretransplant CMV seropositivity and increasing recipient age were independent risk factors for CMV pneumonitis. By contrast, donor CMV seropositivity was protective against disease, supporting the hypothesis that immunity against CMV can be adopted from donor T cell-depleted marrow. It is concluded that detection of CMV viraemia in a surveillance protocol can predict subsequent CMV pneumonitis but that it is of low sensitivity. This might be improved by more frequent sampling or by the use of a more sensitive assay, such as the polymerase chain reaction, thus identifying individuals who may benefit from 'pre-emptive' therapy.
- Published
- 1993
117. The prognostic significance of positive CMV cultures during surveillance of renal transplant recipients
- Author
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Paul Sweny, SF Liu, D. Pillay, A. A. Ali, E Kops, and Paul D. Griffiths
- Subjects
Ganciclovir ,medicine.medical_specialty ,Cytomegalovirus ,Gastroenterology ,Betaherpesvirinae ,Internal medicine ,medicine ,Humans ,Viral shedding ,Kidney transplantation ,Whole blood ,Antilymphocyte Serum ,Transplantation ,biology ,business.industry ,Interferon-alpha ,biology.organism_classification ,medicine.disease ,Prognosis ,Kidney Transplantation ,Virus Shedding ,Relative risk ,Immunology ,Cytomegalovirus Infections ,Viral disease ,business ,Immunosuppressive Agents ,medicine.drug - Abstract
Renal transplant recipients are at risk of severe morbidity and mortality from CMV disease. We have undertaken routine surveillance for CMV shedding on 133 transplant recipients, using a rapid culture technique, in order to assess the incidence of CMV infection and disease in these patients and to assess the prognostic significance of detection in whole blood, throat swab specimens, or urine. Donor CMV seropositivity was associated with posttransplant CMV infection (P < 0.05) and disease (P = 0.06). CMV infection and disease were associated with the receipt of anti-T-cell antibodies (P < 0.0001 and P = 0.08, respectively). First shedding of virus from any site occurred earlier posttransplant in those recipients who developed disease (median 39 days) than in those who did not (median 55 days)(P < 0.05). Detection of virus in blood occurred at a median time of 16 days before onset of symptoms, compared with 9 days before symptoms in urine, and 3 days after onset of symptoms from throat swab. A positive blood culture represented a relative risk of 7.1 for the subsequent development of disease, compared with 2.1 and 1.8 for positive urine and saliva cultures, respectively. The addition of urine cultures to blood cultures increased the sensitivity for identification of those at risk--however, the relative risk was reduced to 5.8. We conclude that routine surveillance for CMV shedding, especially in blood and urine, can identify recipients at high risk of CMV disease, and propose a trial in which those with asymptomatic viremia are allocated to receive ganciclovir or placebo, in order to assess the efficacy of "preemptive" therapy in this group of patients.
- Published
- 1993
118. A clinico-pathological audit of opportunistic viral infections in HIV-infected patients
- Author
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D. Pillay, Christine A. Lee, Mark C.I. Lipman, Paul D. Griffiths, James E. McLaughlin, and Margaret A. Johnson
- Subjects
Adult ,Diarrhea ,Male ,Pathology ,medicine.medical_specialty ,Opportunistic infection ,medicine.medical_treatment ,Immunology ,JC virus ,HIV Infections ,medicine.disease_cause ,Herpesviridae ,Virus ,Adenovirus Infections, Human ,Acquired immunodeficiency syndrome (AIDS) ,London ,Immunology and Allergy ,Medicine ,Humans ,Tissue Distribution ,Aged ,AIDS-Related Opportunistic Infections ,business.industry ,Immunosuppression ,Herpes Simplex ,Middle Aged ,medicine.disease ,JC Virus ,Tumor Virus Infections ,Infectious Diseases ,Cytomegalovirus Infections ,Viruses ,Encephalitis ,Female ,Viral disease ,Autopsy ,business - Abstract
Objective: To determine the prevalence of opportunistic viral infections in multiple tissues at postmortems of HIV-infected patients, and to relate these findings to their antemortem clinical course.Design: A study of viral infections in 16 tissues of HIV-positive postmortem cases, by a combination of histology and cell culture (virus isolation). Clinical details were abstracted retrospectively from patient records.Patients: Forty-seven consecutive autopsies, performed between 1985 and 1992.Setting: Autopsies were conducted by a single pathologist in a single London teaching hospital.Results: Opportunistic viral infections were detected in 72% of all cases, comprising cytomegalovirus (CMV, 66%), herpes simplex virus (11%), JC virus (6%) and adenovirus (2%). The most commonly infected tissues were lung, adrenal, gastrointestinal tract and central nervous system, although all tissue sites sampled could potentially support viral replication. Of 464 tissues tested by both histology and cell culture, histology alone detected CMV in 45 tissues and cell culture alone detected CMV in 31 tissues. We determined that CMV detection in postmortem gastrointestinal tissues and central nervous tissue was significantly associated with antemortem undiagnosed diarrhoea and encephalitis, respectively.Conclusion: There is a high prevalence of opportunistic viral infections in late-stage HIV disease, which is best detected postmortem by the use of both histology and cell culture. Many of these infections correlate with undiagnosed symptoms antemortem. The ability of sensitive methods for virus detection to alert the clinician to such cases antemortem should be critically evaluated, as should attempts to influence the natural history of these infections by antiviral drugs. Continuing clinico-pathological audit is important for AIDS patients in order to monitor the impact of known opportunistic viral infections and to identify others which may emerge as immunosuppression becomes more profound.
- Published
- 1993
119. Characterization of a nested polymerase chain reaction assay for detection of parvovirus B19
- Author
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D. Pillay, J Pattison, S. Myint, and G. Patou
- Subjects
Microbiology (medical) ,viruses ,Molecular Sequence Data ,Radioimmunoassay ,Dot blot ,Erythema Infectiosum ,In situ hybridization ,Antibodies, Viral ,Peripheral blood mononuclear cell ,Polymerase Chain Reaction ,Sensitivity and Specificity ,law.invention ,Serology ,law ,hemic and lymphatic diseases ,Parvovirus B19, Human ,Humans ,Longitudinal Studies ,Polymerase chain reaction ,In Situ Hybridization ,biology ,Base Sequence ,Parvovirus ,virus diseases ,Nucleic Acid Hybridization ,biology.organism_classification ,Virology ,Molecular biology ,Blood ,Immunoglobulin M ,Immunoglobulin G ,DNA, Viral ,biology.protein ,Leukocytes, Mononuclear ,Pharynx ,Nested polymerase chain reaction ,Research Article - Abstract
The characterization and application of a nested polymerase chain reaction (PCR) assay for the detection of human parvovirus B19 DNA is described. The assay was evaluated with 149 diagnostic serum samples (collected up to 150 days after the onset of symptoms) previously tested by dot blot hybridization for B19 DNA and by class-specific capture radioimmunoassays for the detection of B19 immunoglobulin M (IgM) and IgG. B19 DNA was detectable by the PCR in 70% of the sera. There was a statistically significant association between the detection of B19 DNA by PCR and high B19 IgM values (P < 0.005), low B19 IgG values (P < 0.05), and a short interval between onset of symptoms and serum collection (P < 0.005). Serial serum samples, throat swabs, and peripheral blood mononuclear cells collected from 10 individuals during an outbreak of parvovirus B19 were also tested by the nested PCR. B19 DNA was detectable in the throat swabs at the time of the clinical illness and in the peripheral blood mononuclear cell fraction up to the end point of the study 6 months after infection. The location of the B19 DNA could not be determined in cytocentrifuge preparations of peripheral blood mononuclear cells with nonisotopic in situ hybridization and immunolabelling.
- Published
- 1993
120. Detection of cytomegalovirus by a rapid culture system: a comparison of monoclonal antibodies in a clinical setting
- Author
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Paul D. Griffiths, D. Pillay, Heather Charman, and Jennie Lok
- Subjects
biology ,medicine.drug_class ,Congenital cytomegalovirus infection ,Antibodies, Monoclonal ,Cytomegalovirus ,biology.organism_classification ,medicine.disease ,Monoclonal antibody ,medicine.disease_cause ,Antibodies, Viral ,Virology ,Antiviral Agents ,Sensitivity and Specificity ,Herpesviridae ,Antigen ,Betaherpesvirinae ,Cell culture ,Monoclonal ,Immunology ,Cytomegalovirus Infections ,biology.protein ,medicine ,Humans ,Antibody - Abstract
A monoclonal antibody cocktail, directed against immediate early antigens of cytomegalovirus, was assessed in a rapid culture system for detection of CMV (DEAFF) in clinical samples. The antibody cocktail was shown to be equivalent in sensitivity and specificity to our currently used monoclonal antibody. A comparison of DEAFF (using either monoclonal preparation) and conventional cell culture shows that DEAFF detects a smaller proportion of CMV positive samples from those receiving antiviral chemotherapy. The reasons for the discrepancies observed between these techniques are discussed.
- Published
- 1992
121. Risk factors for viral reactivation following bone marrow transplantation
- Author
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D. Pillay, A. D. B. Webster, H. G. Prentice, and Paul D. Griffiths
- Subjects
Herpesvirus 3, Human ,Time Factors ,viruses ,Population ,Congenital cytomegalovirus infection ,medicine.disease_cause ,Antibodies, Viral ,Herpesviridae ,Betaherpesvirinae ,Risk Factors ,medicine ,Humans ,Simplexvirus ,Risk factor ,education ,Bone Marrow Transplantation ,education.field_of_study ,biology ,Transmission (medicine) ,Hematology ,General Medicine ,biology.organism_classification ,medicine.disease ,Transplantation ,Immunology ,Virus Activation ,Viral disease - Abstract
The identification of risk factors for viral reactivation following transplantation is essential in order that antiviral prophylactic regimes may be allocated rationally. In the pretransplant period qualitative serological assessment of recipient and donor is informative with regard to the risk of post-transplant reactivation and disease. In addition, the quantitative level of herpes simplex virus (HSV) IgG in the pretransplant recipient is predictive of post-transplant HSV excretion, although this relationship does not exist for other viruses of the herpes group. We discuss the value of surveillance cultures for cytomegalovirus in the post-transplant period in predicting the development of CMV disease, and how this may allow effective intervention to prevent what is a major cause of morbidity and mortality in the transplant population.
- Published
- 1992
122. Diagnosis of cytomegalovirus infection: a review
- Author
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D. Pillay and Paul D. Griffiths
- Subjects
biology ,business.industry ,Congenital cytomegalovirus infection ,virus diseases ,Retinitis ,Cytomegalovirus ,Context (language use) ,Dermatology ,Disease ,biology.organism_classification ,medicine.disease ,medicine.disease_cause ,Prognosis ,Herpesviridae ,Infectious Diseases ,Betaherpesvirinae ,Virology ,Immunology ,Cytomegalovirus Infections ,medicine ,Humans ,Viral disease ,business ,Encephalitis ,Research Article - Abstract
Introduction Cytomegalovirus (CMV) infects between 40100% of adults throughout the world, depending primarily on socio-economic status. However, other relevent factors include the degree and form of sexual activity such that, in the UK, homosexual men have a seroprevalence of 80% compared with 42% of sexually active male heterosexuals.' These individuals may present to genitourinary medicine clinics with CMV-related symptoms, most of whom will be infected with HIV. As a member of the herpes group of viruses, infection with CMV leads to a state of latency with the capacity to reactivate at any time. Primary infection in the immunocompetent is usually asymptomatic but may be associated with a mononucleosis-type illness. Reactivation is of major consequence in those who are immunocompromised in whom it may be associated with retinitis, colitis, pneumonitis or encephalitis, all of which carry significant morbidity.2 The availability of effective anti-CMV therapy has heightened the requirement for rapid, sensitive methods of CMV detection, and these will be discussed in detail. By contrast, serological diagnosis of CMV infection (ie antibody determination) depends on a functional immune system and, we believe, plays little role in CMV diagnosis in the immunocompromised individual. Despite the severe symptomatology associated with CMV, it is important to realise that infection does not equate with disease. Thus, virological evidence of CMV infection must always be interpreted within the clinical context before treatment is considered.
- Published
- 1992
123. Surveillance for CMV infection in orthotopic liver transplant recipients
- Author
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Keith Rolles, Mark Smith, D. Pillay, Paul D. Griffiths, Heather Charman, and Andrew K. Burroughs
- Subjects
Adult ,Blood transfusion ,medicine.medical_treatment ,Biopsy ,Congenital cytomegalovirus infection ,Cytomegalovirus ,Disease ,medicine.disease_cause ,Virus ,Herpesviridae ,Betaherpesvirinae ,Risk Factors ,medicine ,Humans ,Cells, Cultured ,Transplantation ,biology ,business.industry ,Immunosuppression ,Middle Aged ,medicine.disease ,biology.organism_classification ,Liver Transplantation ,Blood ,Immunology ,Cytomegalovirus Infections ,Virus Activation ,Viral disease ,business - Abstract
Infection with cytomegalovirus is a major cause of morbidity and mortality following orthotopic liver transplantation. In order that preventive strategies may be devised, a detailed assessment of risk factors for infection and disease is required. We have prospectively assessed 46 orthotopic liver transplant recipients for CMV excretion from multiple sites in order to determine incidence of, and risk factors for, CMV infection and disease. Risk factors for posttransplant CMV infection were donor CMV seropositivity, an increased volume of peritransplant whole-blood transfusion and an increased dose of extra steroid therapy to treat rejection episodes. These findings implicate, respectively, transfer of virus with donor organ, transfer of virus with blood transfusion, and stimulation of reactivation of latent virus in recipients through augmented immunosuppression. The possible ways of preventing or ameliorating these effects are discussed.
- Published
- 1992
124. Parvovirus B19 outbreak in a children's ward
- Author
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S. Hurt, C.C. Kibbler, D. Pillay, P.D. Griffiths, and G. Patou
- Subjects
Adult ,Male ,Pediatrics ,medicine.medical_specialty ,Anemia ,viruses ,Erythema Infectiosum ,Antibodies, Viral ,Serology ,Disease Outbreaks ,Sex Factors ,London ,Immune Tolerance ,Parvovirus B19, Human ,Medicine ,Humans ,Child ,Index case ,Parvoviridae ,biology ,Parvovirus ,business.industry ,virus diseases ,Obstetrics and Gynecology ,Outbreak ,General Medicine ,medicine.disease ,biology.organism_classification ,Virology ,Personnel, Hospital ,Immunoglobulin M ,Immunoglobulin G ,Sick leave ,Immunology ,Female ,Viral disease ,business ,Hospital Units - Abstract
Parvovirus B19 infection can cause severe complications in pregnant women, individuals with haemolytic anaemia, and those who are immunocompromised. In a hospital outbreak of this infection, a balance should be struck between protection of these individuals and the maintenance of medical services. The index case of an outbreak of parvovirus B19 infection among staff and patients of a paediatric ward was not identified. 58 members of staff were screened for B19 markers and 4 of the 6 susceptible men and 6 of the 24 susceptible women became infected (p = 0·05) as defined by serum IgM and viraemia. 1 of the 11 adults (10 members of staff and 1 parent) infected remained symptom-free. 12 immunocompromised patients were also assessed, and symptom-free infection developed in 2 of these. During the outbreak staff with symptoms were put on sick leave, immunocompromised patients (there were none with haemolytic anaemia) were given normal human immunoglobulin and nursed in single rooms by B19 IgG-positive, IgM-negative staff, and the ward was closed to B19 IgG-negative pregnant women. However, the limitation of spread of infection cannot be attributed with certainty to the measures taken.
- Published
- 1992
125. Secondary parvovirus B19 infection in an immunocompromised child
- Author
-
P.D. Griffiths, D. Pillay, Gary Patou, and Lesley Rees
- Subjects
Microbiology (medical) ,Male ,medicine.medical_treatment ,Molecular Sequence Data ,Parvoviridae Infections ,Viremia ,Opportunistic Infections ,Antibodies, Viral ,Virus ,Parvoviridae ,Immunopathology ,medicine ,Immune Tolerance ,Humans ,Child ,biology ,Base Sequence ,Parvovirus ,business.industry ,Immunosuppression ,medicine.disease ,biology.organism_classification ,Virology ,Kidney Transplantation ,Infectious Diseases ,Pediatrics, Perinatology and Child Health ,Immunology ,DNA, Viral ,Viral disease ,business - Published
- 1991
126. Cytomegalovirus excretion after bone marrow transplantation cannot be predicted from the pre-transplant level of IgG antibodies
- Author
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D. Lee, D. Pillay, A. D. B. Webster, Paul D. Griffiths, and H. G. Prentice
- Subjects
Adult ,Adolescent ,viruses ,Congenital cytomegalovirus infection ,Cytomegalovirus ,medicine.disease_cause ,Antibodies, Viral ,Herpesviridae ,Serology ,Excretion ,Betaherpesvirinae ,Risk Factors ,Virology ,Preoperative Care ,medicine ,Humans ,Child ,Bone Marrow Transplantation ,biology ,business.industry ,virus diseases ,Middle Aged ,biology.organism_classification ,medicine.disease ,surgical procedures, operative ,Infectious Diseases ,medicine.anatomical_structure ,Herpes simplex virus ,Child, Preschool ,Immunoglobulin G ,Immunology ,Cytomegalovirus Infections ,biology.protein ,Bone marrow ,Antibody ,business - Abstract
The relationship of pre-bone-marrow transplant recipient and donor cytomegalovirus (CMV) antibody level to post-transplant recipient CMV excretion was investigated. CMV excretion was found to be related to pre-transplant recipient serological status, but not to level of antibody. This therefore contrasts with herpes simplex virus (HSV), for which pre-transplant antibody level in recipients predicts post-transplant HSV excretion.
- Published
- 1991
127. Influence of atenolol on within-subject variation of thyroid function tests
- Author
-
Gerald F. Watts, P R N Kind, and D Pillay
- Subjects
Adult ,Male ,medicine.medical_specialty ,Clinical Biochemistry ,Reference range ,Thyroid Function Tests ,Thyroid function tests ,chemistry.chemical_compound ,Thyroid-stimulating hormone ,Internal medicine ,medicine ,Humans ,Euthyroid ,Angina, Unstable ,Aged ,Creatinine ,medicine.diagnostic_test ,business.industry ,Thyroid ,General Medicine ,Middle Aged ,Atenolol ,medicine.anatomical_structure ,Endocrinology ,chemistry ,Analysis of variance ,business ,medicine.drug - Abstract
Atenolol is a commonly used beta-blocker that may affect serum thyroid hormone concentrations.' However its influence on the wellrecognized within-subject variation':' of the free levels of thyroxine (free T4), tri-iodothyronine (free T3) and thyroid stimulating hormone (TSH) has not been previously documented. Knowledge of this effect may be important when monitoring thyroid status in relevant patients, Twelve clinically euthyroid men (mean age 53 years, range 37-64; mean wt 80 kg, 61-95), who were receiving 100mg of atenolol daily for stable angina, and a closely matched group (n = 12; mean age 48 years, 35-61; mean wt 79 kg, 59-99) not receiving atenolol were studied. Serum levels of free T4 (Amerlex-M RIA, reference range 8·525·0 pmol/L), free T3 (Amerlex-M RIA, reference range 2·6-8,0 pmol/L) and TSH (Amerlite RIA, reference range 0·3-4'OmU/L) were measured in the cases and controls on four separate occasions (six-month intervals) over an 18-month period; inter-assay CVs 4-6%. None of the patients from either group suffered major illness, received additional medication, or showed a significant change in body weight during the study; serum creatinine remained < 120J1.mol/L. Compliance with atenolol was confirmed by direct questioning and occasional packet counts, The values of FT4, FT3 and TSH were compared between the groups by Student's t-test. Within-subject variance was estimated by analysis of variance and compared between the groups using the F-test;4 this analysis was not factored for analytical variance. The ratio of within-subject variance (including analytical variance) to total variance was also calculated, Although there were no significant differences in the mean levels of the three hormones between the groups, the within-subject variance of FT4 and FT 3 was significantly greater in the atenololtreated patients than in controls (see Table I), With the control group, the ratio of withinsubject variance to total variance for FT4, FT3 and TSH was 25, 45 and 25%, respectively, By contrast, with atenolol treatment the ratio of variances for FT4, FT 3 and TSH increased by a factor of 2,2'1, and 1,8, respectively. However, even after allowing for analytical variance, the within-subject variance for FT3 in this group was higher than that reported by Browning et al? This might have been due to differences in the characteristics of the sample population and to the smaller number of observations made in the present study
- Published
- 1990
128. Effect of ketones and glucose on the estimation of urinary creatinine: implications for microalbuminuria screening
- Author
-
Gerald F. Watts and D. Pillay
- Subjects
medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Urinary system ,Renal function ,Hydroxybutyrates ,Urine ,Ketone Bodies ,Acetoacetates ,chemistry.chemical_compound ,Endocrinology ,Glycosuria ,Diabetes mellitus ,Internal medicine ,Internal Medicine ,medicine ,Albuminuria ,Humans ,Creatininase ,Creatinine ,Autoanalysis ,3-Hydroxybutyric Acid ,business.industry ,medicine.disease ,chemistry ,Ketone bodies ,Microalbuminuria ,business - Abstract
The influence of acetoacetate, 3-hydroxybutyrate, and glucose on the assay of urinary creatinine by Jaffe-type and enzymatic methods was studied in vitro. Acetoacetate (greater than or equal to 5 mmol l-1) led to significant overestimation of low concentration urinary creatinine measured by an end-point and a kinetic Jaffe reaction, but neither 3-hydroxybutyrate nor glucose influenced these reactions. None of the metabolites interfered with an enzymatic creatininase assay. It is recommended that, when employing the urinary albumin/creatinine ratio to screen for microalbuminuria in diabetes mellitus, an enzymatic method be used to measure creatinine in Ketostix positive samples, particularly those that are dilute or obtained from patients with a low lean body mass.
- Published
- 1990
129. Aciclovir resistance among indian strains of Herpes simplex virus as determined using a dye uptake assay
- Author
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D Pillay, Gopalan Sridharan, S. Kavitha, Jambulingam Malathi, Mary V. Jesudason, P Joseph, Asha Mary Abraham, and Rahul George
- Subjects
Microbiology (medical) ,business.industry ,viruses ,Human immunodeficiency virus (HIV) ,virus diseases ,medicine.disease_cause ,Virology ,Microbiology ,Herpes simplex virus ,Dye uptake ,medicine ,Aciclovir ,skin and connective tissue diseases ,business ,medicine.drug - Abstract
Resistance to aciclovir (ACV) among Herpes simplex virus (HSV) isolates is increasingly being reported in the literature particularly in immunocompromised patients. However, there is only limited data available from India despite widespread use of ACV in our hospital. A cross-sectional study was hence conducted to determine the aciclovir (ACV) susceptibility of HSV 1 and 2 isolates using a dye uptake (DU) assay. This study showed a 3.0% prevalence of ACV resistance among HSV-1 strains (2/66, median IC 50 0.098 µg/mL) while in HSV-2 strains, it was 7.8% (5/64, median IC 50 0.195 µg/mL). The IC 50 for the HSV-1 and HSV-2 strains resistant to ACV was greater than or equal to 6.25 µg/mL.
- Published
- 2007
130. Gold standard reduction of MRSA by silver?
- Author
-
P.P. De and D. Pillay
- Subjects
Microbiology (medical) ,Reduction (complexity) ,Infectious Diseases ,business.industry ,Medicine ,General Medicine ,Gold standard (test) ,business ,Nuclear chemistry - Published
- 2005
131. The prevalence of hepatitis G virus in high risk patient groups
- Author
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S.J. Skidmore, D. Pillay, and K.E. Collingham
- Subjects
Microbiology (medical) ,medicine.medical_specialty ,High risk patients ,business.industry ,Internal medicine ,medicine ,business ,Molecular Biology ,Microbiology ,Virus ,Hepatitis G - Published
- 1997
132. Book review: Antiviral chemotherapy 4, Advances in experimental medicine and biology Vol 394 Ed. by J. Mills, P. A. Volberding and L. Corey, Plenum Press (1996)
- Author
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D. Pillay
- Subjects
Gerontology ,Infectious Diseases ,Antiviral chemotherapy ,business.industry ,Virology ,Medicine ,business - Published
- 1997
133. In vitro screening of sugarcane to evaluate smut susceptibility.
- Author
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N. Singh, B. M. Somai, and D. Pillay
- Published
- 2005
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134. An open-label study of tenofovir in HIV-1 and Hepatitis B virus co-infected individuals.
- Author
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M. Nelson, S. Portsmouth, J. Stebbing, M. Atkins, A. Barr, G. Matthews, D. Pillay, M. Fisher, M. Bower, and B. Gazzard
- Published
- 2003
- Full Text
- View/download PDF
135. Viral Infections, Contemporary Issues in Infectious Diseases. K. Root Richard & E. Sande Merle. Pp. 218+20 figs. Edinburgh: Churchill Livingstone; 1992
- Author
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D. Pillay
- Subjects
medicine.medical_specialty ,Pathology ,Infectious Diseases ,Epidemiology ,business.industry ,medicine ,Intensive care medicine ,business - Published
- 1993
136. Virus induced enzymes, J. M. Morrison, John Wiley and Sons, Chichester (1991). Hardback, 655 pp. £85.00; $180.85. ISBN 0471 923 397
- Author
-
D. Pillay
- Subjects
chemistry.chemical_classification ,Infectious Diseases ,Enzyme ,chemistry ,Virology ,Philosophy ,Molecular biology ,Virus - Published
- 1992
137. Multiscale drivers of hippopotamus distribution in the St Lucia Estuary, South Africa.
- Author
-
A. S., Prinsloo, D., Pillay, and O'Riain, M. J.
- Subjects
- *
HIPPOPOTAMUS , *BIOLOGICAL invasions , *INTRODUCED animals , *COLONIZATION (Ecology) , *ESTUARIES , *POPULATION viability analysis - Abstract
A recent introduction of the hippopotamus Hippopotamus amphibius into Colombia is a novel introduction of a megaherbivore onto a new continent, and raises questions about the future dynamics of the socio-ecological system into which it has been introduced. The hippo Hippopotamus amphibius, native to Africa, has invaded extensive areas of the Magdalena River basin (Colombia) over the past decades, and has been considered the largest invasive animal in the world. The results are discussed in terms of how these findings relate to wild hippo activity, current knowledge of zoo-housed hippo welfare, and future directions for zoo-housed hippo welfare and research. [Extracted from the article]
- Published
- 2021
138. Preparation and Characterization of Membranes from a Coryneform Isolated from Ratoon Stunted Sugarcane
- Author
-
R. A. Oellermann, D. Pillay, G. Roth, and A. Seetal
- Subjects
Antiserum ,Sucrose ,Physiology ,Plant Science ,Biology ,Precipitin ,Immunodiffusion ,chemistry.chemical_compound ,Membrane ,chemistry ,Biochemistry ,Genetics ,Density gradient ultracentrifugation ,Lysozyme ,Agronomy and Crop Science ,Polyacrylamide gel electrophoresis - Abstract
Membranes from the coryneform were prepared by an optimized chemical procedure using EDTA and lysozyme and isolated upon equilibrium sucrose density gradient ultracentrifugation. Four bands, I to IV, of peak densities 1.15, 1.19, 1.21 and 1.245 g. cm−3 respectively, were reproducibly obtained. Immunodiffusion of the isolated membrane bands against bacterial antiserum showed that the immunological integrity of the isolated membranes was retained. Membrane bands I and II had similar precipitin arcs having one major component. A shift towards greater immunological complexity in band IV was observed. Similarly, a change in polypeptide composition established by polyacrylamide gel electrophoresis was found. The major group of polypeptides inband I ranging in size from 39 to 67 kdal were successively reduced in bands II, III and IV whereas the polypeptide of 15.7 kdal and of those in the region of 140 kdal showed respective increases. Transmission electron microscopy revealed similar membrane, structures in all membrane bands with only minor differences between them.
- Published
- 1987
139. Enzyme-Linked Immunosorbent Assay (ELISA) of a Coryneform Isolated from Ratoon Stunted Sugarcane
- Author
-
R. A. Oellermann and D. Pillay
- Subjects
chemistry.chemical_classification ,biology ,Physiology ,Coryneform bacterium ,Plant Science ,biology.organism_classification ,Diluent ,Microbiology ,fluids and secretions ,Enzyme ,Incubation temperature ,chemistry ,Antigen ,Genetics ,biology.protein ,Antibody ,Agronomy and Crop Science ,Bacteria ,Conjugate - Abstract
An enzyme-linked immunosorbent assay (ELISA) was developed for the coryneform bacterium isolated from ratoon stunted surgarcane. Parameters governing the ELISA were optimized. These included antibody coating concentration, conjugate concentration and diluent, antigen diluent, the washing procedure, incubation temperature for sample and conjugate, pH and time. The level of detectability of the cultured bacterium by the ELISA was ≥ 1 × 104 Zellen · ml-1.
- Published
- 1988
140. Effects of Insulin and Maternal Diabetes on Fetal Lipogenesis in the Rat
- Author
-
D Pillay and E Bailey
- Subjects
medicine.medical_specialty ,Offspring ,medicine.medical_treatment ,Pregnancy in Diabetics ,Maternal diabetes ,Biochemistry ,Diabetes Mellitus, Experimental ,Fetus ,Adipose Tissue, Brown ,Pregnancy ,Internal medicine ,Brown adipose tissue ,Animals ,Insulin ,Medicine ,Maternal-Fetal Exchange ,business.industry ,Hormone sensitivity ,Rats, Inbred Strains ,medicine.disease ,Lipids ,Rats ,Endocrinology ,medicine.anatomical_structure ,Liver ,Lipogenesis ,Female ,business - Abstract
Offspring of diabetic mothers have been investigated with regard to fetal hepatic and brown adipose tissue lipogenesis in the rat. Results, which cannot be explained by existing theory, are obtained from offspring of subdiabetic mothers and manifest diabetic mothers. In re-evaluating the effect of exogenous insulin on perinatal lipogenesis, we find important differences in hormone sensitivity between liver and brown adipose tissue.
- Published
- 1983
141. Immunological Methods for the Detection of a Coryneform Isolated from Ratoon Stunted Sugarcane
- Author
-
D. Pillay and R. A. Oellermann
- Subjects
Antiserum ,Specific antiserum ,Hemagglutination ,medicine.diagnostic_test ,Physiology ,Coryneform bacterium ,Plant Science ,Biology ,biology.organism_classification ,Immunofluorescence ,Microscopic observation ,Microbiology ,Agglutination (biology) ,Genetics ,medicine ,Agronomy and Crop Science ,Bacteria - Abstract
A coryneform bacterium, isolated from ratoon stunted sugarcane, has been obtained in pure culture. Its identification by immunological means was investigated. Optimum conditions for the light microscopic observation of bacterial agglutination by specific antiserum, immunofluorescence and the microcapillary haemagglutination of IgG-sensitized erythrocytes by the bacterium were established. All three assay procedures are simple and rapid. By comparison, more samples can be handled at any one time bythe microcapillary haemagglutination method. Based on bacterial agglutination, the antiserum is specific for the coryneform isolated from ratoon-stunted sugarcane.
- Published
- 1987
142. Acute liver graft failure due to emergence of lamivudine resistant hepatitis B virus: rapid resolution during treatment with adefovir.
- Author
-
D, Mutimer, H, Feraz-Neto B, R, Harrison, K, O'Donnell, J, Shaw, P, Cane, and D, Pillay
- Abstract
BACKGROUND: Strategies for prevention of liver graft reinfection by hepatitis B virus (HBV) have been developed during recent years. Initially, passive immunoprophylaxis with high titre HBV immunoglobulin (HBIg), followed by lamivudine prophylaxis, and then the combination of lamivudine and HBIg have been employed. However, suboptimal use of the combination may be associated with failure of prophylaxis reflected by the emergence of HBV species with genetic changes that confer resistance to lamivudine and HBIg. Reinfection of the graft by HBV can be associated with rapid development of liver failure. CASE REPORT: A 43 year old HBV infected man received lamivudine before transplantation, and lamivudine and HBIg after transplantation. Despite prophylaxis, graft reinfection and severe hepatitis were observed. The observed serological evolution and genetic sequencing of the emergent HBV species suggested selection of lamivudine resistant and surface antigen escape mutants consecutively. Adefovir treatment began after the development of graft failure. OUTCOME: A rapid exponential decline in serum HBV titre was observed. Liver function tests normalised and signs of liver failure resolved. CONCLUSION: The use of HBIg and lamivudine permits prevention of graft reinfection by HBV for the majority of patients. Adefovir, a potent inhibitor of lamivudine resistant HBV, should be used when failure of prophylaxis is associated with graft hepatitis.
- Published
- 2001
143. Outcome of lamivudine resistant hepatitis B virus infection in the liver transplant recipient.
- Author
-
D, Mutimer, D, Pillay, P, Shields, P, Cane, D, Ratcliffe, B, Martin, S, Buchan, L, Boxall, K, O'Donnell, J, Shaw, S, Hbscher, and E, Elias
- Abstract
BACKGROUND: In many transplant centres lamivudine is an important component of prophylaxis against, and treatment of, hepatitis B virus (HBV) graft infection. Drug resistant HBV species with specific polymerase mutations may emerge during lamivudine treatment. AIMS: To examine the clinical consequences of graft infection by lamivudine resistant virus. METHODS: The clinical course of four liver transplant patients who developed graft infection with lamivudine resistant virus was reviewed. The response of HBV infection to reduction of immunosuppression and to manipulation of antiviral therapy was assessed. For each patient, serum viral titre was measured and the viral polymerase gene was sequenced at multiple time points. RESULTS: High serum titres were observed following emergence of the lamivudine resistant species. Wild type HBV re-emerged as the dominant serum species after lamivudine withdrawal. All patients developed liver failure, and onset of liver dysfunction was observed when resistant virus was the dominant serum species. In three patients, liver recovery was observed when immunosuppression was stopped and when alternative antivirals were given. Wild type virus appeared to respond to ganciclovir, and to reintroduction of lamivudine. For one patient, introduction of famciclovir was associated with clinical, virological, and histological response. CONCLUSIONS: Failure of lamivudine prophylaxis may identify patients at special risk for the development of severe graft infection. Treatment of graft reinfection should include reduction of immunosuppression, and systematic exposure to alternative antivirals. Viral quantitation and genetic sequencing are essential components of therapeutic monitoring.
- Published
- 2000
144. White Laager: the rise of Afrikaner nationalism by W. H. Vatcher Jr New York, Praeger, 1965. Pp. x + 309. $6.95
- Author
-
P. D. Pillay
- Subjects
White (horse) ,Sociology and Political Science ,media_common.quotation_subject ,Geography, Planning and Development ,Afrikaner nationalism ,Art ,Theology ,media_common - Published
- 1965
145. The effect of maternal diet on maternal and fetal hepatic and brown adipose tissue lipogenesis and blood and tissue metabolites
- Author
-
F C, Higham, D, Pillay, and E, Bailey
- Subjects
Adipose Tissue ,Adipose Tissue, Brown ,Liver ,Pregnancy ,Starvation ,Animals ,Female ,Rats, Inbred Strains ,Fatty Acids, Nonesterified ,Fetal Blood ,Lipids ,Diet ,Rats - Abstract
Both starvation of and feeding a high linoleic acid content diet to rats during late pregnancy resulted in (relative to stock diet fed controls) a decrease in maternal hepatic glycogen content and lipogenesis, reduced blood glucose levels and an increase in blood ketone levels whereas only fat feeding caused an increase in blood triglyceride levels and only starvation resulted in a decrease in the amount of brown adipose tissue. Maternal starvation resulted in decreased fetal body, liver and brown adipose tissue weights, diminished liver and brown adipose glycogen content and lipogenesis and lowered blood glucose levels. Both maternal fat feeding and starvation caused triglyceride accumulation in fetal liver, elevated blood ketone levels and an increased proportion of fetal liver and carcass fatty acids accounted for by linoleic acid.
- Published
- 1984
146. Electrophoretic and immunological analysis of lipopolysaccharides of Xanthomonas albilineans from three geographical regions
- Author
-
Lise Korsten, E. A. Wachters, B. Pillay, and D. Pillay
- Subjects
Gel electrophoresis ,Xanthomonas albilineans ,Silver Staining Method ,Pseudomonadales ,lipids (amino acids, peptides, and proteins) ,Gamma globulin ,Biology ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Pathogen ,Bacteria ,Pseudomonadaceae ,Microbiology - Abstract
Lipopolysaccharides (LPS) were extracted from the type species of the sugarcane leaf scald pathogen Xanthomonas albilineans from Fiji and additional isolates from Australia, Mauritius and South Africa. After resolution by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, the LPS were visualized using a modified silver staining method and analysed by densitometric scanning. Three distinct electrophoretic patterns were identified: the LPS pattern of the Fijian isolate differed from those of the other isolates. Immunological cross-reactivity of the LPS and gamma globulins was assessed by immunoblotting. Immunoblotting showed that although IgG raised against other isolates cross-reacted with the LPS of one another, they did not react with the purified LPS of the Fijian isolate. Differences in LPS patterns and serological cross-reactivity may be due to differences in the length and conformation of the O-polysaccharide chains of the LPS.
147. From Protest to Challenge: A Documentary History of African Politics in South Africa, 1882-1964. Volume I: Protest and Hope, 1882-1934. Edited by Gwendolen M. Carter and Thomas Karis
- Author
-
P. D. Pillay
- Subjects
History ,Politics ,Political economy ,media_common.quotation_subject ,Economic history ,Institution ,Sociology ,media_common - Published
- 1973
148. Antiviral drug resistance.
- Author
-
D, Pillay and M, Zambon
- Published
- 1998
149. Colonial Sequence 1949 to 1969: A Chronological Commentary upon British Colonial Policy in Africa
- Author
-
P. D. Pillay and Margery Perham
- Subjects
History ,Political Science and International Relations ,Colonialism ,Genealogy ,Sequence (medicine) - Published
- 1973
150. O112 Trends in transmitted HIV drug resistance among non-B subtypes in the UK
- Author
-
S. Lattimore, Hannah Green, D Chilton, Valerie Delpech, D Pillay, and Linda Harrison
- Subjects
Resistance test ,medicine.medical_specialty ,Nevirapine ,business.industry ,Transmission (medicine) ,Public health ,Human immunodeficiency virus (HIV) ,Public Health, Environmental and Occupational Health ,Drug resistance ,medicine.disease_cause ,Virology ,Drug class ,Infectious Diseases ,Immunology ,medicine ,business ,HIV drug resistance ,medicine.drug - Abstract
Background Europe is seeing more non-B subtype HIV, reflecting patterns of migration. In France in 2003, 19% of new infections were non-B subtype [1] and UK data showed a rise in infection acquired heterosexually in Africa: 19% in 1992, to 33% in 2006 [2]. With the roll-out of antiretrovirals (ART) in the developing world and use of single dose nevirapine for prevention of mother-to-child transmission, reports of emerging transmitted drug resistance (TDR) among non-B subtypes have followed.
- Full Text
- View/download PDF
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