Search

Your search keyword '"Zhang, Jinghai"' showing total 342 results
Start Over Author "Zhang, Jinghai"
342 results on '"Zhang, Jinghai"'

67. Methionine 58 is a key residue in the modulation of BmK scorpion toxin AGP-SYPU2 activity through in silicoand in vivostudies

Author

Cui, Yong, Wang, Ting, Hao, Zhihui, Zhang, Jinghai, and Zhao, Yongshan

Abstract

AbstractProtein dynamic networks play an important role in the regulation of many protein systems. Some residues that are far away from the interface between proteins and their targets have a critical role in modulating the activity of some scorpion toxins. Here, conservation analysis combined with an in vivo experiment has reveals that Met58 is a key residue of BmK scorpion toxin AGP-SYPU2 in the modulation of analgesic activity. Molecular dynamics simulations clearly reveal the conformational changes that allow the loop between the β2 and β3 sheets to be exposed on the toxin surface to interact with its targets. Our results emphasize specific roles for the residue Met58 in the NC domain and our work gives valuable information for further modification of scorpion toxins to obtain new analgesic peptides with enhanced activity. Communicated by Ramaswamy H. Sarma

Published
2022
Full Text
View/download PDF

74. Voltage‐gated sodium channels β3 subunit promotes tumorigenesis in hepatocellular carcinoma by facilitating p53 degradation.

Author

Li, Shuai, Han, Jiadi, Guo, Guili, Sun, Yudi, Zhang, Tingting, Zhao, Mingyi, Xu, Yijia, Cui, Yong, Liu, Yanfeng, and Zhang, Jinghai

Subjects
SODIUM channels, HEPATOCELLULAR carcinoma, P53 protein, CELL cycle, TUMOR growth, UBIQUITINATION
Abstract

The voltage‐gated sodium channels (VGSCs) are aberrantly expressed in a variety of tumors and play an important role in tumor growth and metastasis. Here, we show that VGSCs auxiliary β3 subunit, encoded by the SCN3B gene, promotes proliferation and suppresses apoptosis in HepG2 cells by promoting p53 degradation. β3 significantly increases HepG2 cell proliferation, promotes tumor growth in mouse xenograft models, and suppresses senescence and apoptosis. We found that β3 knockdown stabilizes p53 protein, leading to potentiation of p53‐induced cell cycle arrest, senescence, and apoptosis. Mechanistic studies revealed that β3 could bind to p53, promoting p53 ubiquitination and degradation by stabilizing the p53/MDM2 complex. Our results suggest that β3 is a novel negative regulator of p53 and a potential oncogenic factor. [ABSTRACT FROM AUTHOR]

Published
2020
Full Text
View/download PDF

78. A mutant of the Buthus martensii Karsch antitumor-analgesic peptide exhibits reduced inhibition to hNav1.4 and hNav1.5 channels while retaining analgesic activity

Author

Xu, Yijia, primary, Meng, Xiangxue, additional, Hou, Xue, additional, Sun, Jianfang, additional, Kong, Xiaohua, additional, Sun, Yuqi, additional, Liu, Zeyu, additional, Ma, Yuanyuan, additional, Niu, Ye, additional, Song, Yongbo, additional, Cui, Yong, additional, Zhao, Mingyi, additional, and Zhang, Jinghai, additional

Published
2017
Full Text
View/download PDF

80. A novel pattern recognition protein of the Chinese oak silkmoth, Antheraea pernyi, is involved in the pro-PO activating system

Author

Li Wei, Ding Guoyuan, Ma Youlei, Chen Ying, Zhang Rong, Zhao Mingyi, Wu Chunfu, Zou Wenjun, Wang Xialu, and Zhang Jinghai

Subjects
China, Recombinant Fusion Proteins, Molecular Sequence Data, Sequence alignment, Antheraea pernyi, Moths, Biochemistry, lcsh:Biochemistry, C-type lectin, Complementary DNA, Hemolymph, Escherichia coli, Animals, Lectins, C-Type, lcsh:QD415-436, Amino Acid Sequence, RNA, Messenger, Cloning, Molecular, lcsh:QH301-705.5, Molecular Biology, Peptide sequence, Research Articles, Innate immunity, Phosphotransferases (Phosphate Group Acceptor), Innate immune system, Base Sequence, biology, business.industry, Pattern recognition, General Medicine, biology.organism_classification, Immunity, Innate, Pattern recognition protein, Open reading frame, Pro-PO activation, lcsh:Biology (General), Artificial intelligence, business, Sequence Alignment, Protein Binding
Abstract

In this paper, we firstly reported a C-type lectin cDNA clone of 1029 bps from the larvae of A. Pernyi (Ap-CTL) using PCR and RACE techniques. The full-length cDNA contains an open reading frame encoding 308 amino acid residues which has two different carbohydrate-recognition domains (CRDs) arranged in tandem. To investigate the biological activities in the innate immunity, recombinant Ap-CTL was expressed in E. coli with a 6-histidine at the amino-terminus (Ap-rCTL). Besides acted as a broad-spectrum recognition protein binding to a wide range of PAMPs and microorganisms, Ap-rCTL also had the ability to recognize and trigger the agglutination of bacteria and fungi. In the proPO activation assay, Ap-rCTL specifically restored the PO activity of hemolymph blocked by anti-Ap-rCTL antibody in the presence of different PAMPs or microorganisms. In summary, Ap-rCTL plays an important role in insect innate immunity as an pattern recognition protein. [BMB Reports 2013; 46(7): 358-363]

Published
2013

86. Complex network sampling based on particle swarm optimization

Author

Pan Feng, Li Weixing, Zhang Jinghai, Gao Qi, and Hu Yang

Subjects
Continuous optimization, Mathematical optimization, Meta-optimization, Optimization problem, Derivative-free optimization, Sampling (statistics), Particle swarm optimization, Multi-swarm optimization, Metaheuristic, Mathematics
Abstract

Whether the sampling subnets can accurately represent the topology and dynamics of the original networks is an important research topic. To improve the quality of network sampling, this paper attempts to convert the complex network sampling process to an optimization problem, and proposes a novel sampling algorithm which is based on Particle Swarm Optimization (PSO). Exponent of power-law degree distribution and clustering coefficient of networks were set as optimization objectives. Subnets were sampled from scale-free network by random sampling method, and optimization objectives were optimized by multi-objective optimizer. Kolmogorov-Smirnov test is used to verify that whether the sampling subnets conform to strict power-law degree distribution. Simulations show that the algorithm based on intelligent optimization methods could get better sample subnets than normal sampling algorithm. The optimization objectives of the sampling algorithm proposed in this paper could be extended to other statistical properties of complex network, and the alternative algorithm other than random sampling could also be used.

Published
2015

87. Differential proteomic analysis of the anti-depressive effects of oleamide in a rat chronic mild stress model of depression

Author

Rong Zhang, Jing Shen, Fang Wang, Lin Ge, Jingyu Yang, Zhang Jinghai, Chunfu Wu, Ming-ming Zhu, and Hui-Fang Tian

Subjects
Male, Proteomics, medicine.medical_specialty, Oleamide, Protein subunit, Clinical Biochemistry, Neuropeptide, Adenylate kinase, Nerve Tissue Proteins, Oleic Acids, Biology, Toxicology, Biochemistry, Hippocampus, GSTA4, Rats, Sprague-Dawley, Behavioral Neuroscience, chemistry.chemical_compound, Internal medicine, Biogenic amine, Fluoxetine, medicine, Initiation factor, Animals, Electrophoresis, Gel, Two-Dimensional, Biological Psychiatry, Pharmacology, chemistry.chemical_classification, Depression, Glutathione, Antidepressive Agents, Rats, Disease Models, Animal, Endocrinology, chemistry, Biomarkers, Selective Serotonin Reuptake Inhibitors, Stress, Psychological
Abstract

Depression is a complex psychiatric disorder, and its etiology and pathophysiology are not completely understood. Depression involves changes in many biogenic amine, neuropeptide, and oxidative systems, as well as alterations in neuroendocrine function and immune-inflammatory pathways. Oleamide is a fatty amide which exhibits pharmacological effects leading to hypnosis, sedation, and anti-anxiety effects. In the present study, the chronic mild stress (CMS) model was used to investigate the antidepressant-like activity of oleamide. Rats were exposed to 10weeks of CMS or control conditions and were then subsequently treated with 2weeks of daily oleamide (5mg/kg, i.p.), fluoxetine (10mg/kg, i.p.), or vehicle. Protein extracts from the hippocampus were then collected, and hippocampal maps were generated by way of two-dimensional gel electrophoresis (2-DE). Altered proteins induced by CMS and oleamide were identified through mass spectrometry and database searches. Compared to the control group, the CMS rats exhibited significantly less body weight gain and decreased sucrose consumption. Treatment with oleamide caused a reversal of the CMS-induced deficit in sucrose consumption. In the proteomic analysis, 12 protein spots were selected and identified. CMS increased the levels of adenylate kinase isoenzyme 1 (AK1), nucleoside diphosphate kinase B (NDKB), histidine triad nucleotide-binding protein 1 (HINT1), acyl-protein thioesterase 2 (APT-2), and glutathione S-transferase A4 (GSTA4). Compared to the CMS samples, seven spots changed significantly following treatment with oleamide, including GSTA4, glutathione S-transferase A6 (GSTA6), GTP-binding nuclear protein Ran (Ran-GTP), ATP synthase subunit d, transgelin-3, small ubiquitin-related modifier 2 (SUMO2), and eukaryotic translation initiation factor 5A-1 (eIF5A1). Of these seven proteins, the level of eIF5A1 was up-regulated, whereas the remaining proteins were down-regulated. In conclusion, oleamide has antidepressant-like properties in the CMS rat model. The identification of proteins altered by CMS and oleamide treatment provides support for targeting these proteins in the development of novel therapies for depression.

Published
2014

Catalog

Books, media, physical & digital resources
See catalog results