51. Tissues and cells involved in the invasion of the rabbit intestinal tract by sporozoites of Eimeria coecicola
- Author
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F Drouet-Viard, Dominique Kerboeuf, Yves Le Vern, P. Coudert, Michal Pakandl, Nathalie K. Chanteloup, Sophie Renaux, Station de Pathologie aviaire et parasitologie [Nouzilly] (PAP), Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration
- Subjects
Pathology ,medicine.medical_specialty ,T-Lymphocytes ,Lymphocyte ,030231 tropical medicine ,Spleen ,Cell Separation ,Biology ,Host-Parasite Interactions ,030308 mycology & parasitology ,Flow cytometry ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,In vivo ,medicine ,Animals ,Mesenteric lymph nodes ,[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology ,ComputingMilieux_MISCELLANEOUS ,B-Lymphocytes ,0303 health sciences ,General Veterinary ,medicine.diagnostic_test ,Coccidiosis ,General Medicine ,Flow Cytometry ,Molecular biology ,Small intestine ,3. Good health ,Intestines ,Infectious Diseases ,medicine.anatomical_structure ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Insect Science ,Eimeria ,Parasitology ,Rabbits ,CD5 - Abstract
This study was designed to identify an extra-intestinal route of migration of Eimeria coecicola sporozoites and the types of cell harbouring the parasite during the invasion of the intestine. The presence of E. coecicola in blood, spleen and mesenteric lymph nodes of infected donor rabbits was demonstrated by immunohistology on donor organs and measurement of oocyst excretion by coccidia-free recipient rabbits injected with whole-cell suspensions prepared from donor tissues. Two types of donor lymphocyte, B (IgM+) and T (CD5+), were labelled using a two-colour immunofluorescence-labelling technique and separated with a cell-sorter (FACStar(Plus)). The presence of parasites in the sorted cells was assessed by direct examination and by using the same in vivo test after intravenous injection of IgM+ B or CD5+ T lymphocytes collected from donors at different times after inoculation. This test provided evidence that the parasites were alive and still infectious within the sorted lymphocytes. It was demonstrated that both B and T lymphocytes were infected.
- Published
- 2001