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Enzymatic effect of hypodermin A, a parasite protease, on bovine lymphocyte membrane antigens
- Source :
- Parasite Immunology, Parasite Immunology, Wiley, 1997, 19 (1), pp.21-27
- Publication Year :
- 1997
- Publisher :
- HAL CCSD, 1997.
-
Abstract
- The protease hypodermin A (HA) is produced by the parasitic warble-fly larva and is implicated in the modulation of the bovine immune system. This study examines the effect of this enzyme on the cell surface markers of bovine lymphocytes. HA interfered with the binding of all anti-lymphocyte receptor antibodies tested. Anti-BoCD2 and CD5 staining was completely abolished. But the mean fluorescence intensity (MFI) only was diminished for antibodies against BoCD4, CD8 and CD18. On the contrary, the MFI for anti-MHC Cl I molecules staining was increased. This effect of HA began as early as one h, and was reversed by removal of HA. Heating or PMSF treatment, which both inhibit protease activity, abolished the action of HA on the surface antigens. The HA concentrations (100 micrograms/ml) needed to alter antibody binding were similar to those that inhibited phytohaemagglutinin (PHA)-induced proliferation. These results show that enzymatic activity of HA on lymphocyte surface markers may be implicated in the inhibition of lymphocyte proliferation.
- Subjects :
- [SDV.IMM] Life Sciences [q-bio]/Immunology
HYPODERMINE A
medicine.medical_treatment
Lymphocyte
030231 tropical medicine
Immunology
Lymphocyte proliferation
Lymphocyte Activation
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Antigen
Antigens, CD
medicine
Animals
Lymphocytes
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
Phytohaemagglutinin
Serine protease
0303 health sciences
Protease
biology
Dose-Response Relationship, Drug
Diptera
Serine Endopeptidases
Membrane Proteins
Molecular biology
Immunohistochemistry
3. Good health
medicine.anatomical_structure
chemistry
Larva
biology.protein
[SDV.IMM]Life Sciences [q-bio]/Immunology
Parasitology
Cattle
PMSF
Antibody
Subjects
Details
- Language :
- English
- ISSN :
- 01419838 and 13653024
- Database :
- OpenAIRE
- Journal :
- Parasite Immunology, Parasite Immunology, Wiley, 1997, 19 (1), pp.21-27
- Accession number :
- edsair.doi.dedup.....b4d697223e51e8d9986973cee391e6df