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Abstract Background Patients with acute lung injury (ALI) have increased levels of pro-inflammatory mediators, which impair endothelial progenitor cell (EPC) function. Increasing the number of EPC and alleviating EPC dysfunction induced by pro-inflammatory mediators play important roles in suppressing ALI development. Because the high density lipoprotein reverse-D-4F (Rev-D4F) improves EPC function, we hypothesized that it might repair lipopolysaccharide (LPS)-induced lung damage by improving EPC numbers and function in an LPS-induced ALI mouse model. Methods LPS was used to induce ALI in mice, and then the mice received intraperitoneal injections of Rev-D4F. Immunohistochemical staining, flow cytometry, MTT, transwell, and western blotting were used to assess the effect of Rev-D4F on repairment of lung impairment, and improvement of EPC numbers and function, as well as the signaling pathways involved. Results Rev-D4F inhibits LPS-induced pulmonary edema and decreases plasma levels of the pro-inflammatory mediators TNF-α and ET-1 in ALI mice. Rev-D4F inhibited infiltration of red and white blood cells into the interstitial space, reduced lung injury-induced inflammation, and restored injured pulmonary capillary endothelial cells. In addition, Rev-D4F increased numbers of circulating EPC, stimulated EPC differentiation, and improved EPC function impaired by LPS. Rev-D4F also acted via a PI3-kinase-dependent mechanism to restore levels of phospho-AKT, eNOS, and phospho-eNOS suppressed by LPS. Conclusions These findings indicate that Rev-D4F has an important vasculoprotective role in ALI by improving the EPC numbers and functions, and Rev-D4F reverses LPS-induced EPC dysfuncion partially through PI3K/AKT/eNOS signaling pathway.

Currently available Raman scanners are limited in speed to acquire images of clinically relevant sizes in cancer imaging. Here, the authors developed a DNA based design principle for Raman-Fluorescence bimodal nanoparticles and demonstrate real-time, high precision image-guided tumor resections and photothermal ablation of cancer.

Cherangani hills, located in Western Kenya, comprises of 12 forest blocks, maintaining great plant diversity. However, little attention to plant diversity studies has been paid to it in the past years. Here, we present a checklist of the vascular plants of this region obtained through intensive field investigations and matching of herbarium specimens. In total, 1296 species, including 17 endemic species are documented, belonging to 130 families and 608 genera. This flora represents 18.50%, 43.83% and 54.17% of the Kenyan species, genera and families, respectively. The habit, habitat and voucher specimens, as well as brief notes on the distribution of each taxon recorded are presented in this checklist. It is the first exhaustive inventory of the terrestrial vascular plants in Cherangani hills which is a significant regional centre for plant diversity.

Hadal trenches remain one of the unexplored ocean ecosystems due to the challenges of sampling at great depths. It is still unclear how a faunal community changes from the abyssal to the hadal zone, and which environmental variables are the key impacting factors. In this study, nine dives of the Human Occupied Vehicle (HOV) “JIAOLONG” were conducted from abyssal to hadal depths (4,435–6,796 m) in the Yap Trench on the southeastern boundary of the Philippine Sea Plate in the western Pacific, divided into 48,200 m video transects, to describe the megafaunal communities and reveal their relationship with environmental factors. A total of 1,171 megafauna organisms was recorded, 80 morphospecies (msps) from 8 phyla were identified based on the video data, most of which were reported for the first time in the Yap Trench. Arthropoda was the most abundant phylum and Echinodermata was the most diverse phylum of the megafaunal community. The faunal abundance increased with depth, whereas the Shannon diversity index decreased with depth. Cluster analysis suggested seven assemblages, with five abyssal groups, one mixed group, and one hadal dominant group. Although megafaunal communities changed gradually from abyssal zone to hadal zone, both PERMANOVA and PERMDISP analyses revealed that the communities are significantly different between abyssal zone and hadal zone, indicating 6,000 m as the boundary between the two depth zones. Depth, substrate, slope, and latitude were identified as four important environmental factors with significant influence on megafaunal community structure. This study proposed a transition pattern from the abyssal to hadal zone in the Yap Trench, highlighted the importance of habitat heterogeneity in structuring megafaunal community in a hadal trench.

Magnetorheological gel (MRG) excels in the material properties in term of adjustability and sedimentation performance, which could upgrade the performances of the current magnetorheological fluid based adjustable devices for structural control and vibration mitigation. However, the characterization and modelling of the stress-strain hysteresis responses of MRG has not been reported in the past, which are fundamental steps towards engineering applications. In this study, the stress-strain hysteresis of polyurethane based MRG sample with 60% carbonyl iron particle weight fraction was characterized under sinusoidal shear excitations with broad ranges of strain amplitude (5%–100%), excitation frequency (0.1 Hz–2 Hz) and magnetic fields (0–0.91 T). Significant stress overshooting phenomenon were observed under the application of low magnetic fields (0.27 T). A structurally-simple and accurate phenomenological model has been established to capture this unique nonlinearity. By validating the experimental results, the proposed model accurately predicts the hysteretic behaviour and the overshoot of the MRG under the excitation scenarios and the magnetic fields considered. Finally, the support vector machine (SVM) was implemented to provide the solution to the model generalization. The SVM-assisted model showed good agreement with the experimental data and can benefit the efficiency and viability in developing controllable MRG-based devices and system.

Seamounts are important deep ocean entities that serve as reservoirs for varied types of habitats and fauna. During the Chinese cruises in the northwestern Pacific seamount areas, a new starfish species of order Brisingida, Astrolirus patricki, was found at 1,458–2,125 m depth. All specimens of the new species were observed in situ attaching on hexactinellid sponges, suggesting a possible close relationship between the two taxa. A. patricki sp. nov. is the second known species of the genus, characterized by the abutting plates in the intercostal integument, separated first pair of adambulacral plates and densely distributed proximal spines. Phylogenetic analyses were conducted for order Brisingida to incorporate the new species as well as Hymenodiscus cf. fragilis (Fisher, 1906), Freyella cf. attenuata Sladen, 1889 and two Brisinga spp., for which we present the molecular data for the first time. Phylogenetic trees suggest a close relationship between A. patricki sp. nov. with Brisinga species rather than with Hymenodiscus species, which is inconsistent with morphological taxonomy. This study highlights the distinct morphological and ecological characters of the new species and provides new data for future investigation on Brisingida phylogeny.

Biodiversity of hydrothermal vents in the Indian Ocean, particularly those on the Southwest Indian Ridge (SWIR), are still relatively poorly understood. The Tiancheng field on the SWIR was initially reported with only a low-temperature diffuse flow venting area, but here we report two new active areas, including a chimney emitting high-temperature vent fluids. Biological sampling in these new sites doubled the known megafauna and macrofauna richness reported from Tiancheng. Significantly, we found several iconic species, such as the scaly-foot snail and the first Alviniconcha population on the SWIR. Tiancheng shares a high proportion of taxa with vents on the Central Indian Ridge (CIR) and lacks a number of key taxa that characterize other vents investigated so far on the SWIR. Population genetics of the scaly-foot snail confirmed this, as the Tiancheng population was clustered with populations from the CIR, showing low connectivity with the Longqi field. Unlike the previously examined populations, scales of the Tiancheng scaly-foot snail were coated in zinc sulfide, although this results only from precipitation. The close connection between Tiancheng and CIR vents indicates that the dispersal barrier for vent endemic species is not the Rodriguez Triple Junction as previously suggested but the transformation faults between Tiancheng and Longqi, warranting further studies on deep currents in this area to resolve the key barrier, which has important implications for biological conservation.

Here we present the complete mitochondrial genome of Megabalanus tintinnabulum. The genome is 15,107 bp in length with a 67.35% AT content. It contains 13 protein-coding genes (PCGs), 2 rRNAs genes, and 22 tRNAs. Both rRNAs are encoded on the light strand, as in the other crustacean and barnacle mitochondrial genomes. Besides five tRNAs are encoded on the light strand (nad1, trnV, trnL1, trnC, trnQ, and trnK). Only one PCG is encoded on the light strand (nad1), whereas the other 12 PCGs are located on the heavy strand, which is consistent with M. ajax. Phylogenetic analysis based on mitochondrial PCGs shows that M. tintinnabulum is clustered with M. ajax into a branch (BP = 100), and the group with M. volcano with high support. This study contributes to further phylogenetic analysis within Cirripedia.

Kenya, an African country with major higher plant diversity, has a corresponding diversity of plant associations, because of the wide geographic distribution, diverse climatic conditions and soil types. In this article, all vascular plants of Kenya were counted based on the completed "Flora of Tropical East Africa (FTEA)", and all families and genera were revised using recent molecular systematics research, forming a "Synoptic List of Families and Genera of Kenyan Vascular Plants (SLFGKVP)". In total, there are 225 families, 1538 genera and 6293 indigenous species and and 62 families, 302 genera and 588 exotic species in Kenya. The Fabaceae with 98 genera and 576 Species is the largest family. Two of the seven plant distribution regions of Kenya, K4 and K7 are the most species-richest areas with regard to both total and endemic species, with 3375 and 3191 total species and 174 and 185 endemic species in K4 and K7 respectively. While, K3 and K5 have the highest density of both total and endemic species. K1 has the lowest density of total species, and K2 has the lowest density of endemic species.

Bird-strike failure of fan blades is one of the basic challenges for the safety of aircraft engines. Simplified flat blade-like plates are always used for damage mechanism study of composite laminates. One undesirable issue is the failure at the root of clamped flat plates under high-velocity impact. For this purpose, two different strategies were exploited to obtain desirable impact damage distributions, namely the impact location and the boundary condition. Numerical models of the simplified flat blade-like plate and the bird projectile were constructed by using finite element method (FEM) and smoothed particle hydrodynamics (SPH) approaches. The impact damage distributions were comparatively investigated in detail. The numerical results show that changing the boundary condition is the most effective way to obtain preferable impact damages for further failure analysis of real fan blades. Present results will be useful to the future surrogate experimental design of simplified bird-strike testing.

A new species of the deep-sea spongicolid genus Spongicoloides Hansen, 1908 is described and illustrated based on material from the northwestern Pacific. Spongicoloides weijiaensis sp. n. was found inside a hexactinellid sponge, Euplectella sp., sampled by the Chinese manned submersible “Jiaolong” at depths of 2279 m near the Weijia Guyot, in the Magellan Seamount Chain. The new species can be distinguished from all congeneric species by several morphological features, involving gill formula, spination of the carapace, antennal scale, third pereiopod, telson and uropod, posteroventral teeth of the pleura, and dactyli of the fourth and fifth pereiopods. An identification key to the Pacific species of Spongicoloides is provided.

Online social networks (OSNs) gradually integrate financial capabilities by enabling the usage of real and virtual currency. They serve as new platforms to host a variety of business activities, such as online promotion events, where users can possibly get virtual currency as rewards by participating in such events. Both OSNs and business partners are significantly concerned when attackers instrument a set of accounts to collect virtual currency from these events, which make these events ineffective and result in significant financial loss. It becomes of great importance to proactively detecting these malicious accounts before the online promotion activities and subsequently decreases their priority to be rewarded. In this paper, we propose a novel system, namely ProGuard, to accomplish this objective by systematically integrating features that characterize accounts from three perspectives including their general behaviors, their recharging patterns, and the usage of their currency. We have performed extensive experiments based on data collected from the Tencent QQ, a global leading OSN with built-in financial management activities. Experimental results have demonstrated that our system can accomplish a high detection rate of 96.67% at a very low false positive rate of 0.3%.

Osedaxrubiplumus(Annelida, Siboglinidae)uses heterotrophic bacteria to feed onvertebrate carcasses and is currently found in the Pacific, Antarctic and Indian Ocean.Here, we report its nearly complete mitochondrial genomes assembled for 2 individuals, one from the East Pacific and the other from the Southwest Indian Ocean. Recoveredmitogenomes were 15591 and 15972 bp in length, with both consisting of 37 typical metazoan mitochondrial genes. All genes were transcribed from the same strand, and arranged in the same order as the other siboglinids, revealing conserved gene arrangement withinSiboglinidae. Phylogeneticanalysis of 13 protein coding genes confirms the placement of Osedaxsister to the Vestimentifera+Sclerolinum clade.

Austinograea species are restricted to hydrothermal vents and are typically considered to be omnivorous predators in vent communities. Here we present the complete mitochondrial genome of Austinograea sp. which was collected from Carlsberg Ridge, the mid-ocean ridge in the northwestern Indian Ocean. The genome is 15,584 bp in length with a 68.11% AT content. It contains 13 protein-coding genes (PCGs), 2 ribosomal RNA genes, and 22 transfer RNA genes. Phylogenetic analysis shows that the present species is closest to Austinograea rodriguezensis. This study contributes to further phylogenetic analysis within Eubrachyura.

Compared to species richness, few studies have investigated the patterns and relationship of phylogenetic and functional structures along elevational gradients. Here, we used the general additive models to determine the trends of taxonomic diversity (species richness, SR), phylogenetic and functional diversity (PD and FD), phylogenetic structure net relatedness index (NRI), and functional structure net functional relatedness index (NFRI) of seed plants along the elevational gradient in Mount Kenya, a tropical mountain in Africa. We measured growth form, fruit type, maximum height, and maximum leaf size of each species, calculated the phylogenetic signal of each trait, and tested the Pearson correlation coefficients between NRI and NFRI of each trait. Our results showed that SR, PD, and FD decreased gradually along the elevational gradient. NRI exhibited a fluctuating pattern along the elevational gradient, while NFRI of the four functional traits showed noticeably different patterns. We concluded that the relationship between the phylogenetic and functional structures in different functional traits could be congruent or mismatched along the elevational gradient. Compared with relatively conservative categorical traits (e.g., growth form and fruit type), continuous traits (e.g., height and leaf size) have a random or convergent evolutionary pattern. Therefore, they could be more easily affected by the environment and possibly have higher phenotypic plasticity.

Because of the light weight and high strength, composite laminates have many advantages in aircraft structures; however, they are frequently subjected to severe dynamic loadings during flight. To understand the dynamic properties of composite laminated thin plates at the stress scale, this paper studies the stress modal analysis (SMA) of composite laminated thin plates by finite element method (FEM). Firstly, the basic theory on SMA of composite laminates was given from the classical displacement modal analysis. Secondly, a square laminated thin plate was numerically studied to obtain some distribution laws of the stress mode shapes (SMSs) from the layup and stress component perspectives. Then, based on the characteristics of SMSs in different plies, a modified layup configuration was conducted for possible lower magnitude and more uniform distributions of SMSs. Results indicate that ±45° layups can improve the performance of SMSs of the square plate, without excessively decreasing the modal frequencies. Such fact manifests that ±45° layups are critically vital for the dynamic stress reduction of the square composite laminated plates. Modal participation factor and strain energy were evaluated to assist the determination of critical modes. Lastly, the aspect ratio of the composite plate on layup design was considered. Numerical investigation in this study can serve as a preliminary step of SMSs perspective for the analysis and optimization of dynamic composite laminates.

Mechanical joints are the weakest links of composite materials and structures. The aim of this study is to investigate the effect of the pin profiles on the stress concentration and load-carry capacity of the pin-loaded single-lap joints subjected to tensile loading. Non-circular profiles of pins are numerically investigated to pursue a possible stress concentration reduction in both the net-tension and bearing stresses of composite single lap joints. Firstly, by varying the characteristic parameters, various round-corner squares are evaluated in terms of peak stresses. Secondly, the square-like profile is transferred to the racetrack-like one in order to gain optimal bearing stresses. Thirdly, the pin of the racetrack-like profile is investigated compared to the circular one, taking into account the through-thickness distributions of stresses. Lastly, the orthotropic effect and the load-carry capacity of the proposed profile are also discussed. Keywords: Composite single lap joints, Pin-loaded joints, Stress concentration, Net-tension stress, Bearing stress, Non-circular profile

* Correspondence: zhouyd@wbgcas [...]

To obtain some basic laws for bird-strike resistance of composite materials in aeronautical application, the high-velocity impact behaviors of composite laminates with different materials were studied by numerical methods. The smoothed particle hydrodynamics (SPH) and finite element method (FEM) coupling models were validated from various perspectives, and the numerical results were comparatively investigated. Results show that the different composite materials have relatively little effect on projectile deformations during the bird impact. However, the impact-damage distributions can be significantly different for different composite materials. The strength parameters and fracture energy parameters play different roles in different damage modes. Lastly, modal frequency was tentatively used to explain the damage behavior of the composite laminates, for it can manifest the mass and stiffness characteristics of a dynamic structure. The dynamic properties and strength properties jointly determine the impact-damage resistance of composite laminates under bird strike. Future optimization study can be considered from these two aspects.

Composite laminates can potentially reduce the weight of aircrafts; however, they are subjected to bird strike hazards in civil aviation. To handle their nonlinear dynamic behaviour, in this study, the impact damage of composite laminates were numerically evaluated and designed by means of smoothed particle hydrodynamics (SPH) and the finite element method (FEM) to simulate the interaction between bird projectiles and the laminates. Attention was mainly focused on the different damage modes in various laminates’ plies induced by bird impact on a square laminated plate. A continuum damage mechanics approach was exploited to simulate damage initiation and evolution in composite laminates. Damage maps were computed with respect to different ply angles, i.e., 0°, 45° and −45°. The damage distributions were comparatively investigated, and then the ply design was considered for crashworthiness improvement. The results aim to serve as a design guideline for future prototype-scale bird strike studies of complex laminated structures.

In spite of potential advantages for aircraft structures, composite laminates can be subjected to bird-strike hazard in civil aviation. For purpose of future surrogate experiments, in this study, impact-damage equivalency for twisted composite blades is numerically investigated by Smoothed Particle Hydrodynamics (SPH) and finite element method (FEM). Cantilever slender flat plates are usually used for basic impact tests, the impact-damage equivalency is being considered by comparing damage modes and energies of three impact configurations: (1) twisted blade; (2) flat blade (axisymmetric); and (3) inclined flat blade (centrosymmetric). The damage maps and energy variations were comparatively investigated. Results indicate that both symmetrical flat and inclined flat blades can be, to a certain extent, regarded as alternatives for real twisted blades under bird impact; however, both types of blade have their own merits and drawbacks, and hence should be used carefully. These results aim to serve as tentative design guideline for future prototype or model experimental study of laminated blades in real aeronautical structures.

Pseudorimula is a vent-endemic genus found at some hydrothermal vents in West Pacific, North Atlantic, and Indian oceans. Here, we report a nearly complete mitogenome of Pseudorimula sp. (Vetigastropoda, Lepetodriloidea, Lepetodrilidae), which is collected from Tiancheng vent field on the Southwest Indian Ridge. The genome is 16,682 in length, including 13 complete protein-coding genes, two rRNA genes, and 22 tRNA genes. All genes are arranged in the same order as that in the genus Lepetodrilus, revealing a potential conserved gene arrangement in the family Lepetodrilidae. The phylogenetic analysis shows that the Pseudorimula+Lepetodrilus clade is well supported.

The Longqi hydrothermal vent field is the first deep-sea active vent field to be explored on the ultra-slow spreading Southwest Indian Ridge. Although a number of larger taxa has been described or characterised, many smaller and less conspicuous animals remain undescribed. Here, two small (

The importance of atmospheric correction is pronounced for retrieving physical parameters in aquatic systems. To improve the retrieval accuracy of trophic level index (TLI), we built eight models with 43 samples in Wuhan and proposed an improved method by taking atmospheric water vapor (AWV) information and Landsat-8 (L8) remote sensing image into the input layer of radical basis function (RBF) neural network. All image information taken in RBF have been radiometrically calibrated. Except model(a), image data used in the other seven models were not atmospherically corrected. The eight models have different inputs and the same output (TLI). The models are as follows: (1) model(a), the inputs are seven single bands; (2) model(c), besides seven single bands (b1, b2, b3, b4, b5, b6, b7), we added the AWV parameter k1 to the inputs; (3) model(c1), the inputs are AWV difference coefficient k2 and the seven bands; (4) model(c2), the input layers include seven single bands, k1 and k2; (5) model(b), seven band ratios (b3/b5, b1/b2, b3/b7, b2/b5, b2/b7, b3/b6, and b3/b4) were used as input parameters; (6) model(b1), the inputs are k1 and seven band ratios; (7) model(b2), the inputs are k2 and seven band ratios; (8) model(b3), the inputs are k1, k2, and seven band ratios. We estimated models with root mean squared error (RMSE), model(a) > model(b3) > model(b1) > model(c2) > model(c) > model(b) > model(c1) > model(b2). RMSE of the eight models are 12.762, 11.274, 10.577, 8.904, 8.361, 6.396, 5.389, and 5.104, respectively. Model b2 and c1 are two best models in these experiments, which confirms both the seven single bands and band ratios with k2 are superior to other models. Results also corroborate that most lakes in Wuhan urban area are in mesotrophic and light eutrophic states.

Design optimization of dynamic properties, for example, modal frequencies, can be of much importance when structures are exposed to the shock and/or vibration environments. A modal strain based method is proposed for fast design of natural frequencies of plate-like structures. The basic theory of modal strains of thin plates is reviewed. The capability of determining the highly sensitive elements by means of modal strain analysis is theoretically demonstrated. Finite element models were constructed in numerical simulations. Firstly, the application of the proposed method is conducted on a central-massed flat plate which was topologically optimized by the Reference. The results of modal strain analysis at the first mode have good agreement with the results from the topology optimization. Furthermore, some features of the strain mode shapes (SMSs) of the flat plate are investigated. Finally, the SMSs are applied to the optimization of a stiffened plate. Attention is focused on the distributions of the SMSs of the stiffeners, which also shows good agreement with the results from the topology optimization in the previous study. Several higher orders of SMSs are extracted, which can visualize the most sensitive elements to the corresponding modal frequency. In summary, both the theory and simulations validate the correctness and convenience of applying SMSs to dynamic design of plate-like structures.

The complete mitochondrial genome of Kiwa tyleri (Anomura, Chirostyloidea, Kiwaidae) was recovered by next generation sequencing. The mitogenome is 16,865 bp in length and contains 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs) and a 1525bp non-coding AT-rich region. This is the first mitogenome for the family Kiwaidae and the superfamily Chirostyloidea. The inversion of three consecutive genes (16S rRNA, tRNA-Val, 12S rRNA) was first reported for the Anomura. The phylogenetic tree indicated that Kiwaidae was close to Paguroidea and Lithodoidea rather than Galatheoidea.

The design of stiffeners is an effective approach to enhance the stiffness of panel-type structures. However, the stiffness–mass efficiency depends largely on the spacing, orientation, and cross-section of the stiffeners. In order to improve the stiffness–mass efficiency, this article presents a combined use of topology optimization and finite element analysis to the dynamic design of stiffeners for a typical panel. Finite element models of a flat and a stiffened rectangular panel were constructed. Modal analysis was conducted on the two panels to obtain the basic dynamic properties. Topology optimization model of the stiffened panel, so-called initial structure, was built based on the variable density method. According to the loading case and the predominant mode of the system, the objective function was determined to maximize the fundamental frequency of the panel. The stiffeners were chosen as the design domain. Optimal material density distributions of the stiffeners were obtained at different mass fraction. The finite element model of the optimal stiffened panel was built to reanalyze the dynamic properties and responses. An increase in the relative rate of change in the fundamental frequency versus mass was observed between before and after the optimization.

Acoustic fatigue life evaluation is essential for thermal protection structures due to the severe thermo-acoustic load in service. A study on temperaturedependence of acoustic fatigue life for a C/SiC panel is presented in this paper. Effects of temperature on both the structural responses and the S-N curves are investigated. The Dirlik method is adopted to predict the fatigue life of a C/SiC panel at three different temperatures respectively. Significant differences are observed from the results of numerical simulations between the fatigue lives of the panel in the three cases. The temperature-dependence of acoustic fatigue life of a C/SiC panel is verified, and fatigue test of the material needs to be more attentively performed.