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The structure, time-dependent mechanical deformation, and strength characteristics of loess, which is loose and porous with well-developed vertical joints, are greatly affected by the dry-wet cycles, which are attributed to periodic artificial irrigation, rainfall, and water evaporation. To better understand the creep characteristics of loess under the effect of dry-wet cycles, Q2 loess samples obtained from the South Jingyang County, China, were subjected to different dry-wet cycles (0, 5, 10, 15, 20) and sheared in triaxial creep tests. The experimental results revealed that: firstly, the maximum value of the deviatoric stress corresponding to creep failure gradually decreases with an increase in the dry-wet cycles. Secondly, the long-term strength of the loess after dry-wet cycles were obtained through the Isochronous Curve Method. It is found that the long-term strength and the number of dry-wet cycles showed an exponential decreasing relationship. In addition, the creep damage mechanism of loess due to dry-wet cycles is proposed. This study may provide the basis for understanding the mechanical behavior of the loess under the effect of dry-wet cycles, as well as guidelines for the prevention and prediction of loess landslide stability.

Abstract Critical tissue defects frequently result from trauma, burns, chronic wounds and/or surgery. The ideal treatment for such tissue loss is autografting, but donor sites are often limited. Tissue engineering (TE) is an inspiring alternative for tissue repair and regeneration (TRR). One of the current state-of-the-art methods for TRR is gene therapy. Non-viral gene delivery systems (nVGDS) have great potential for TE and have several advantages over viral delivery including lower immunogenicity and toxicity, better cell specificity, better modifiability, and higher productivity. However, there is no ideal nVGDS for TRR, hence, there is widespread research to improve their properties. This review introduces the basic principles and key aspects of commonly-used nVGDSs. We focus on recent advances in their applications, current challenges, and future directions.

Abstract Treatment of full-thickness skin defects poses significant clinical challenges including risk of infection and severe scaring. Silver nanoparticle (NAg), an effective antimicrobial agent, has provided a promising therapeutic method for burn wounds. However, the detailed mechanism remains unknown. Hence, we constructed a metallic nanosilver particles-collagen/chitosan hybrid scaffold (NAg-CCS) and investigated its potential effects on wound healing. In vitro scratch assay, immunofluorescence staining and antibacterial activity of the scaffold were all studied. In vivo NAg-CCS was applied in full-thickness skin defects in Sprague-Dawley (SD) rats and the therapeutic effects of treatment were evaluated. The results showed that NAg at a concentration of 10 ppm accelerated the migration of fibroblasts with an increase in expression of α-smooth muscle actin (α-SMA). Furthermore, in vivo studies showed increased levels of pro-inflammatory and scar-related factors as well as α-SMA, while markers for macrophage activation were up-regulated. On day 60 post transplantation of ultra-thin skin graft, the regenerated skin by NAg-CCS had a similar structure to normal skin. In summary, we demonstrated that NAg-CCS was bactericidal, anti-inflammatory and promoted wound healing potentially by regulating fibroblast migration and macrophage activation, making it an ideal dermal substitute for wound regeneration.

Abstract Background Although drug-eluting stents have dramatically reduced the rates of restenosis and target lesion revascularization, they are associated with stent thrombosis (ST), a catastrophic event likely due to delayed endothelialization and chronic inflammation caused by the polymer and the metal scaffolds. To increase the safety and efficacy of stents, polymer-free dual drug-eluting stents (DDES) have been developed. Methods A total 160 stents (Bare-metal stents (BMS), polymer-free probucol stents (PrES), sirolimus-eluting stents (SES) and DDES) were randomly implanted in the coronary arteries of 80 pigs. 14, 28, 90 and 191 days after implantation, QCA and OCT evaluations were performed in 20 pigs respectively, and the arteries were harvested for scanning electron microscope (SEM), histomorphology, histopathology analyses and for the relative expression of CD31, CD34 and CD133 on mRNA and protein levels. Results At the 14-day time point, there were significant differences in the strut rate coverage (p = 0.011), with greater coverage in the PrES than in the SES group (53.2%vs. 20.3%, p = 0.002). As well, there were no significant differences in the expression of CD31, CD34 and CD133 between groups in mRNA and protein level. Conclusions DDES were as safe as BMS and SES, but they did not further improve the endothelialization of the stented coronary arteries in the porcine model.

Surface Molecularly Imprinted Polymer (SMIP) for selective adsorption of di(2-ethylhexyl) phthalate(DEHP) was prepared on the surface of silica gel which was modified by aminopropyltriethoxysilane and acryloyl chloride in a two-step method. The prepared SMIP was used to prepare Molecularly Imprinted Solid-Phase Extraction (MISPE) column for selective extraction of DEHP from aqueous solution. The results suggested that MISPE column had high affinity and excellent selectivity for DEHP. And adsorption property of MISPE column was significantly superior to the commercial C18 column. The MISPE column was reusable. An analytical method MISPE-HPLC was developed for extraction and determination of the DEHP residues in environmental water samples. The results showed that the linear relationship between the peak area and the concentration of DEHP were as follow: y=2354.6x+1663.5 with a good correlation coefficient (R2=0.9997) and the detection and quantification limits reached 0.012 mg/L and 0.039 mg/L, respectively. The spiked recoveries of DEHP in river water and lake water sample were in the range from 93.3% to 102.3 % at the spiked level of 50~1000 μg/L. The Relative Standard Deviations (RSD) were less than 5.2%.

In realistic systems of coupled oscillators, it is desired to predict the onset of synchronization where the system equations are unknown, raising the need to develop a prediction framework that is model free and fully data driven. We show that this challenging problem can be addressed with machine learning. In particular, exploiting reservoir computing or echo state networks, we employ a “parameter-aware” scheme to train the neural machine using time series acquired from a small number of distinct asynchronous states in the parameter regime prior to the onset of synchronization. The trained machine can then be used to predict the synchronization transition through tuning the control parameter. We demonstrate the power of the machine learning-based framework using two types of synchronization behaviors: Complete synchronization in coupled identical chaotic oscillators and the phase synchronization in coupled nonidentical phase oscillators, which are representative of the collective dynamics in coupled systems. In addition, we design our numerical experiments such that two transition scenarios are covered: Smooth (second-order) and explosive (first-order) transitions that represent the generic types of phase transition in nonlinear physical systems. A remarkable feature is that, for the network systems exhibiting explosive (first-order) transition, the machine learning scheme is capable of predicting not only the locations of the transition points associated with the forward and backward transition paths but also the hysteresis between the two paths.

The Tianjiaba landslide on the Hang Rui expressway was located in the south of Sinan County, Tongren City, Guizhou Province. From the night of 2 July to the morning of 3 July 2011, numerous house cracks, foundation subsidence, and surface cracks suddenly occurred in the Tudiwan and Tianjiaba villages on the north and south sides of the management area. Based on the field geological survey, drilling, pit exploration, surface displacement, fracture observation, and deep displacement monitoring data analysis the deformation characteristics and causes of the Tianjiaba landslide were comprehensively analyzed. In this study, the landslide was divided into three areas, in which fracture group 3 was the most severe. Among the ten inclinometer holes, the sliding surface displacement of No.8 inclinometer hole was the largest, i.e., 25 mm. The sliding surface slopes of fracture groups 1, 2, and 3 were small, i.e., 12.25°, 4.97° and 6.62° to 4.64°, respectively. The results showed that the displacement values of different positions of the landslide were different, and the ground displacement value was larger than the sliding surface displacement value. The maximum displacement of the ground was 242.68 mm, which is larger than the maximum displacement of the inclinometer hole. Because the displacement of the upper part was greater than that of the lower part, bulging cracks formed in the lower part of the landslide. When the displacement of the upper part was less than that of the lower part, tension cracks formed in the lower part of the landslide. According to the surface cracks and displacement values of inclinometer holes on the sliding surface, the landslide was in the initial sliding stage and filling the platform of the management area on the hillside was the main cause of the landslide.

Background: Silicosis is characterized by the accumulation of fibroblasts and the excessive deposition of extracellular matrix. Fibroblast generation via endothelial-mesenchymal transition (EndMT) is one process responsible for this accumulation of fibroblasts. However, the mechanisms underlying EndMT remain unknown. Methods: Human umbilical vein endothelial cells (HUVECs) were exposed to SiO2 (50 µg/cm2). Specific endothelial and mesenchymal markers were evaluated using immunofluorescence and western blot analysis. Functional changes were evaluated by analyzing cell migration and proliferation. LC3-adenovirus transfections were performed, and changes in autophagy were measured using a marker of autophagy. Results: SiO2 induced decreases in the endothelial cell-specific markers in HUVECs while dramatically increasing mesenchymal cell product levels and mesenchymal functions. Although MCPIP1 expression increased in parallel with the increase in specific mesenchymal cell products, the MCPIP1 expression level was not consistent with the observed decrease in specific endothelial marker expression. Autophagy mediated the effects of MCPIP1, as rapamycin and 3-MA enhanced and attenuated the effect of SiO2 on HUVECs, respectively. MAPKs and the PI3K/Akt pathway were involved in the regulation of MCPIP1 by SiO2, and Pyk2 and MLC-2 mediated cell migration. Conclusion: Our findings reveal a new potential function of MCPIP1, suggesting a possible mechanism of fibrosis in pulmonary silicosis.

Neutrophils are essential for host defence and are recruited to sites of inflammation in response to tissue injury or infection. For inflammation to resolve, these cells must be cleared efficiently and in a controlled manner, either by apoptosis or reverse migration. If the inflammatory response is not well-regulated, persistent neutrophils can cause damage to host tissues and contribute to the pathogenesis of chronic inflammatory diseases, which respond poorly to current treatments. It is therefore important to develop drug discovery strategies that can identify new therapeutics specifically targeting neutrophils, either by promoting their clearance or by preventing their recruitment. Our recent in vivo chemical genetic screen for accelerators of inflammation resolution identified a subset of compounds sharing a common chemical signature, the bicyclic benzopyrone rings. Here, we further investigate the mechanisms of action of the most active of this chemical series, isopimpinellin, in our zebrafish model of neutrophilic inflammation. We found that this compound targets both the recruitment and resolution phases of the inflammatory response. Neutrophil migration towards a site of injury is reduced by isopimpinellin and this occurs as a result of PI3K inhibition. We also show that isopimpinellin induces neutrophil apoptosis to drive inflammation resolution in vivo using a new zebrafish reporter line detecting in vivo neutrophil caspase-3 activity and allowing quantification of flux through the apoptotic pathway in real time. Finally, our studies reveal that clinically available ‘cromones’ are structurally related to isopimpinellin and have previously undescribed pro-resolution activity in vivo. These findings could have implications for the therapeutic use of benzopyrones in inflammatory disease.

This paper discussed the estimation of stress-strength reliability parameter R=P(Y

Inn for electric vehicles (EVs) and photovoltaic (PV) is a typical integration of EVs locally consuming renewable energy sources (RES), with features of EV charging, energy storage, and RES consumption. In the existing research works, scholars focus on the energy storage system (ESS) as a medium to charge EVs and send back the power to the utility grid and alleviating the information asymmetry between EV owners and grid dispatching agencies with lower costs of distribution network. In this study, a mixed operational mode based on inn for EV and PV with a replaceable EV battery is analysed. The fragmented energy transferring can shift the load more effectively, balance fluctuation of RES, of great significance to optimise the operation of distribution network system. In summary, a comprehensive model of power flow in distribution network with inn for EV and PV is proposed. Based on an improved IEEE 33-node case, the effects of fragmented energy management mode are analysed. Numerical results show that replacing the full-charged battery for EV is more effective for peak load time-shifting and improving consuming of RES power based on the interaction among RES, ESS, and the replaceable EV battery.

Time-dependent rheological response of geomaterials to thermal treatment is a crucial issue in geothermal energy utilization and deep mineral mining. This response, however, has not yet been fully considered in the existing rheological constitutive models for sandstones. In order to experimentally investigate such responses and establish the associated rheological constitutive model, this study considers the sandstone specimens which have been thermally treated under different temperatures. The triaxial rheological test in conjunction with the scanning electron microscope is employed in the investigation to observe the mechanically and macro-/micromorphologically rheological response. Investigation results show that the thermal treatment induces microcracks and microdefects, and subsequently, they propagate during the creep. As a consequence, the heterogeneous deformation occurs, and macrocracks are present, leading to the irregular fluctuation and mutation in strain over time. A higher temperature contributes to a more severe structure damage and in turn reduces the intactness of sandstones and elevates the rheological response. The investigation allows successful establishment of a three-dimensional constitutive equation considering the instantaneous elastic response to thermal treatment. Based on the equation, a nonlinear visco-elastoplastic rheological constitutive model is developed for sandstones. Comparison with three existing rheological models shows that the model developed in this study could well represent the rheological process of the thermally treated sandstones.

As an efficient approach to controlling the collective dynamics in networked systems, pinning synchronization has been extensively studied in the literature. Different from the existing studies in which the target is normally chosen as the global synchronization state, here we investigate the pinning control of cluster synchronization in regular networks, including the globally connected and ring-structure networks. For globally connected networks, it is found that when a fraction of nodes is pinned, the group of pinning nodes will form a synchronous cluster, while the unpinning nodes are always in the desynchronization state. We conduct a theoretical analysis of the stability of the synchronous cluster, which shows that the critical pinning strength for controlling cluster is inversely proportionally to the uniform coupling strength, but is independent of the number of pinning nodes. For ring-structure networks, however, it is found that the properties of the synchronous clusters, including the sizes and partition of the clusters, are affected by both the number of pinning nodes and the pinning locations. Theoretical analysis on the stabilities of the clusters shows that different from globally connected networks, the introduction of pinnings to the ring-structure network will degenerate the network symmetry, which in turn determines the properties of the clusters.

Reservoir computing systems, a class of recurrent neural networks, have recently been exploited for model-free, data-based prediction of the state evolution of a variety of chaotic dynamical systems. The prediction horizon demonstrated has been about half dozen Lyapunov time. Is it possible to significantly extend the prediction time beyond what has been achieved so far? We articulate a scheme incorporating time-dependent but sparse data inputs into reservoir computing and demonstrate that such rare “updates” of the actual state practically enable an arbitrarily long prediction horizon for a variety of chaotic systems. A physical understanding based on the theory of temporal synchronization is developed.

In this study, a fast object detection algorithm based on binary deep convolution neural networks (CNNs) is proposed. Convolution kernels of different sizes are used to predict classes and bounding boxes of multi-scale objects directly in the last feature map of a deep CNN. In this way, rapid object detection with acceptable precision loss is achieved. In addition, binary quantisation for weight values and input data of each layer is used to squeeze the networks for faster object detection. Compared to full-precision convolution, the proposed binary deep CNNs for object detection results in 62 times faster convolutional operations and 32 times memory saving in theory, what's more, the proposed method is easy to be implemented in embedded computing systems because of the binary operation for convolution and low memory requirement. Experimental results on Pascal VOC2007 validate the effectiveness of the authors’ proposed method.

An automatic gear sorting system based on monocular vision is proposed in this paper. A CCD camera fixed on the top of the sorting system is used to obtain the images of the gears on the conveyor belt. The gears׳ features including number of holes, number of teeth and color are extracted, which is used to categorize the gears. Photoelectric sensors are used to locate the gears׳ position and produce the trigger signals for pneumatic cylinders. The automatic gear sorting is achieved by using pneumatic actuators to push different gears into their corresponding storage boxes. The experimental results verify the validity and reliability of the proposed method and system.

Triaxial rheological tests are performed on mudstones collected from the soft interlayer in the Three Gorges Reservoir Area. In the tests, both dry and saturated conditions are considered, and a complete rheological process is then observed. Based on such laboratory observations of stress-strain, a four-element rheological constitutive model is developed, which is composed of (i) the Hook element, (ii) the Kelvin element, (iii) the viscoelastic-plastic body, and (iv) the nonlinear viscous body (HKCV model). The HKCV model adopts the one-dimensional and three-dimensional equations that are derived. The rheological parameters required are identified, allowing the successful development of the HKCV model. A comparison with the laboratory test observations and the existing model estimates shows that the estimates of the HKCV model are relatively consistent with the observations of the triaxial rheological test. The HKCV model better characterizes the rheological process than the three existing models. However, the HKCV model has the limitation of requiring more parameters than the existing models.

The effect of temperature and pressure, which play important roles in the mechanical properties of rocks during deep energy exploitation, has not been sufficiently studied in the previous rock creep models. In order to investigate thermal effect in creep models, a modified Nishihara rheological model, taking into account the coupled effect of thermal damage and stress, was proposed by combining the theoretical formula for thermal damage of rocks with the modified Nishihara model. The improved model introduces a nonlinear viscous dashpot, which can accurately describe the accelerated rheological phase of rocks. To verify the proposed model, a triaxial rheological experiment was conducted on sandstone subjected to thermal damage (600°C). In addition, the stress-strain curves within whole creep process of the rheological experiment were analyzed. Furthermore, the theoretical curves of the modified Nishihara rheological model were compared with the experimental results. Results showed that the theoretical curves relatively agree well with the experimental data, suggesting that the proposed new model is more preferred to describing the rheological curve of sandstone subjected to thermal damage at different rheological stages, in particular, it is capable of depicting the accelerated rheological stage of the sandstone, providing a good ability to describe the creep behavior of rocks under thermal-mechanical coupling.

This paper analyses the stability of the Duanjiagou landslide on the Bazhong to Guangan Expressway K134−K135 segment in China. The Duanjiagou landslide took place on 4 November 2015. In order to discover the cause of the landslide, we carried out field geological investigations. The indoor physical property experiments were performed by taking the undisturbed soil sample from the borehole cores. To study the strength of the soil, we carried out a saturation direct shear test and saturation residual shear test on sliding zone soil samples. According to the physical properties of soil and the saturated shear strength parameters of sliding zone soil, the stability was analyzed by the landslide force transmission method and numerical simulation method. The results showed that in the initial sliding stage, the safety factor obtained by using the average value of saturated shear strength parameters was in good agreement with the field observation situation. The landslide was at an unstable state. The softening of soil and roadbed excavation at the foot of the slope are the main reasons for landslides.

Para-chloronitrobenzene (p-CNB), a hazardous and toxic substance, is widely used as an intermediary in chemical industries. p-CNB can cause methaemoglobinaemia due to electron-withdrawing properties of the nitro and chlorine groups. We present a case of a 23-year-old man suffering from thermal burns and p-CNB poisoning. In this case, severe methaemoglobinaemia was caused by the absorption of p-CNB through the burn wounds. Despite active treatment, such as the antidote of methylene blue, the patient’s methaemoglobinaemia progressed, with slowly increasing methaemoglobin (MetHb) level. This case highlights the complexity and difficulty of managing this type of injury. To our knowledge, this case can be the first case report describing methaemoglobinaemia induced by p-CNB in a patient with thermal burns.

Objective: This study was designed to evaluate the efficiency and safety of arterial infusions of calcium gluconate to treat hydrofluoric (HF) acid burns of the distal human limbs. Materials and Methods: Eligible patients with HF burn limbs, collected from January 2008 to October 2011, were given the arterial infusion of calcium gluconate into the injured limbs. The measures of pain were conducted before the infusion, immediately after the infusion, 4 h after the infusion, and 2 days after the infusion by the visual analogy score (VAS). If the VAS score was higher than 4.0 at the time point 4 h after the first infusion, the infusion was repeated. The time of wound healing, and the number and ratio of the cases receiving the surgical operation were also evaluated. Results: A total of 118 patients, male (107 cases) and female (11 cases), were collected, including 64 cases of outpatients and 54 cases of inpatients. The age of the subjects ranged from 16 to 60 years, with the mean age of 37.6. The burn sites were located in the lateral limbs (28 cases) and in the unilateral limbs (90 cases). For 107 cases, the pain scores decreased quickly after the first infusion. The other 11 cases, with the VAS score higher than 4.0 at the time point 4 h after the first infusion, received the second infusion. The average time of wound healing and the ratio of the cases receiving the surgical operation were closely related to the interval from the injury to the reception of infusion. Conclusions: Arterial infusion of calcium gluconate, effectively relieving the pain, blocking wound progressive deepening, and causing no adverse effects, could be the preferential method to treat hydrofluoric acid burns of the distal human limbs.

Cell migration is fundamental to the inflammatory response, but uncontrolled cell migration and excess recruitment of neutrophils and other leukocytes can cause damage to the tissue. Here we describe the use of an in vivo model – the Tg(mpx:GFP)i114 zebrafish line, in which neutrophils are labelled by green fluorescent protein (GFP) – to screen a natural product library for compounds that can affect neutrophil migratory behaviour. Among 1040 fungal extracts screened, two were found to inhibit neutrophil migration completely. Subfractionation of these extracts identified sterigmatocystin and antibiotic PF1052 as the active components. Using the EZ-TAXIScan chemotaxis assay, both compounds were also found to have a dosage-dependent inhibitory effect on murine neutrophil migration. Furthermore, neutrophils treated with PF1052 failed to form pseudopods and appeared round in shape, suggesting a defect in PI3-kinase (PI3K) signalling. We generated a transgenic neutrophil-specific PtdIns(3,4,5)P3 (PIP3) reporter zebrafish line, which revealed that PF1052 does not affect the activation of PI3K at the plasma membrane. In human neutrophils, PF1052 neither induced apoptosis nor blocked AKT phosphorylation. In conclusion, we have identified an antibiotic from a natural product library with potent anti-inflammatory properties, and have established the utility of the mpx:GFP transgenic zebrafish for high-throughput in vivo screens for novel inhibitors of neutrophil migration.

Summary Hedgehog (Hh) signaling is mediated by the Gli transcription factors and, in the zebrafish, plays an important role in patterning both the neural tube and myotome. Using a null allele of the gli2a gene induced by targeted mutagenesis, we show that Gli2a is completely dispensable in the fish but acts redundantly with Gli1 to regulate expression of known Hh targets, such as ptch2, prdm1a and eng2a, in the myotome and neural tube. To identify novel targets of Hh signaling, we performed chromatin immunoprecipitation sequencing (ChIP-seq) of whole embryo extracts. Samples were significantly enriched for 192 genomic regions, some of which are associated with four known Hh target genes, ptch1, ptch2, gli1 and olig2. Sequence analysis of these regions reveals a high level of conservation of Gli-binding sites from fish to mammals in some, but not all, cases. Expression analysis of other transcription units that are closely associated with peaks identified several putative targets not previously implicated as Hh targets, including myl10, hnmt, lrp4, efemp2, fras1, quo, and lamc1. Each of these genes shows loss of, or reduced expression in, embryos homozygous for an antimorphic allele of gli2a, you-too (yot), consistent with their being direct targets of Gli2a.

Long-term potentiation (LTP) of synaptic strength is strongly implicated in learning and memory. On the other hand, depotentiation, the reversal of synaptic strength from potentiated LTP state to the pre-LTP level, is required in extinction of the obsolete memory. A generic tristable system, which couples the phosphatase and kinase switches, exclusively explains how moderate and high elevation of intracellular calcium concentration triggers long-term depression (LTD) and LTP, respectively. The present study, introducing calcium influx and calcium release from internal store into the tristable system, further show that significant elevation of cytoplasmic calcium concentration switches activation of both kinase and phosphatase to their basal states, thereby depotentiate the synaptic strength. A phase-plane analysis of the combined model was employed to explain the previously reported depotentiation in experiments and predict a threshold-like effect with calcium concentration. The results not only reveal a mechanism of NMDAR- and mGluR-dependent depotentiation, but also predict further experiments about the role of internal calcium store in induction of depotentiation and extinction of established memories.

The phytohormone abscisic acid (ABA) regulates plant growth, development and responses to biotic and abiotic stresses. The core ABA signaling pathway consists of three major components: ABA receptor (PYR1/PYLs), type 2C Protein Phosphatase (PP2C) and SNF1-related protein kinase 2 (SnRK2). Nevertheless, the complexity of ABA signaling remains to be explored. To uncover new components of ABA signal transduction pathways, we performed a yeast two-hybrid screen for SnRK2-interacting proteins. We found that Type One Protein Phosphatase 1 (TOPP1) and its regulatory protein, At Inhibitor-2 (AtI-2), physically interact with SnRK2s and also with PYLs. TOPP1 inhibited the kinase activity of SnRK2.6, and this inhibition could be enhanced by AtI-2. Transactivation assays showed that TOPP1 and AtI-2 negatively regulated the SnRK2.2/3/6-mediated activation of the ABA responsive reporter gene RD29B, supporting a negative role of TOPP1 and AtI-2 in ABA signaling. Consistent with these findings, topp1 and ati-2 mutant plants displayed hypersensitivities to ABA and salt treatments, and transcriptome analysis of TOPP1 and AtI-2 knockout plants revealed an increased expression of multiple ABA-responsive genes in the mutants. Taken together, our results uncover TOPP1 and AtI-2 as negative regulators of ABA signaling.

This paper proposes a novel breaking strategy for dramatically shortening the response time of a single, stable electromagnetic contactor. A reverse voltage was applied across the excitation coil to increase the decay velocity of the magnetic field in the iron core, leading to a dramatic reduction of the electromagnetic force when the contactor initiated the breaking process. The applied time of the reverse voltage was determined by numerical computation of circuit, magnetic field, and forces. The polarity of source voltage was overturned by controlling switching devices in the bridge drive circuit. A co-simulation coupling magnetic field, machinery, and circuit was carried out using Maxwell and Circuit Editor software. Experimental results are reported to demonstrate the effectiveness of the proposed breaking strategy for shortening the response time of an electromagnetic contactor.

A NiO@ZnO (NZO) hybrid with different reaction times was successfully synthesized by a green hydrothermal method. After comparison, it was found that hydrothermal time had a great impact on specific capacitance. As a supercapacitor electrode of NZO-12h, it exhibited the maximum reversible specific capacitance of 985.0 F/g (3.94 F/cm2) at 5 mA/cm2 and 587.5 F/g (2.35 F/cm2) at 50 mA/cm2, as well as a high retention of 74.9% capacitance after 1500 cycles at 20 mA/cm2. Furthermore, the asymmetric electrode device with ZnO-12h and activated carbon (AC) as the positive and negative electrodes was successfully assembled. In addition, the device exhibited a specific capacitance of 85.7 F/g at 0.4 A/g. Moreover, the highest energy density of 27.13 Wh kg−1 was obtained at a power density of 321.42 W kg−1. These desirable electrochemical properties demonstrate that the NZO hybrid is a promising electrode material for a supercapacitor.

DNA demethylation mediated by the DNA glycosylase ROS1 helps determine genomic DNA methylation patterns and protects active genes from being silenced. However, little is known about the mechanism of regulation of ROS1 enzymatic activity. Using a forward genetic screen, we identified an anti-silencing (ASI) factor, ASI3, the dysfunction of which causes transgene promoter hyper-methylation and silencing. Map-based cloning identified ASI3 as MET18, a component of the cytosolic iron-sulfur cluster assembly (CIA) pathway. Mutation in MET18 leads to hyper-methylation at thousands of genomic loci, the majority of which overlap with hypermethylated loci identified in ros1 and ros1dml2dml3 mutants. Affinity purification followed by mass spectrometry indicated that ROS1 physically associates with MET18 and other CIA components. Yeast two-hybrid and split luciferase assays showed that ROS1 can directly interact with MET18 and another CIA component, AE7. Site-directed mutagenesis of ROS1 indicated that the conserved iron-sulfur motif is indispensable for ROS1 enzymatic activity. Our results suggest that ROS1-mediated active DNA demethylation requires MET18-dependent transfer of the iron-sulfur cluster, highlighting an important role of the CIA pathway in epigenetic regulation.

The responses of complex dynamical systems to external perturbations are of both theoretical importance and practical significance. Different from the conventional studies in which perturbations are added on to the system variables, here we consider the scenario of perturbed couplings. Specifically, by the scheme of periodic coupling in which the coupling strength varies periodically with time, we investigate how the synchronization behaviors of an ensemble of phase oscillators are affected by the properties of periodic coupling, including the coupling frequency and amplitude. It is found that, by comparison with the constant coupling scenario, the presence of period coupling will suppress synchronization in general and, with a decrease in the frequency (or the increase of the amplitude) of the periodic coupling, the synchronization performance gradually deteriorates. The influences of periodic coupling on synchronization are demonstrated by numerical simulations in different network models, and the underlying mechanism is analyzed by the method of dimension reduction.

Detection and tracking strategies based on monocular vision are proposed for autonomous aerial refuelling tasks. The drogue attached to the fuel tanker aircraft has two important features. The grey values of the drogue's inner part are different from the external umbrella ribs, as shown in the image. The shape of the drogue's inner dark part is nearly circular. According to crucial prior knowledge, the rough and fine positioning algorithms are designed to detect the drogue. Particle filter based on the drogue's shape is proposed to track the drogue. A strategy to switch between detection and tracking is proposed to improve the robustness of the algorithms. The inner dark part of the drogue is segmented precisely in the detecting and tracking process and the segmented circular part can be used to measure its spatial position. The experimental results show that the proposed method has good performance in real-time and satisfied robustness and positioning accuracy.

This study investigated compressive strength, chloride penetration, and freeze-thaw resistance of multiwalled carbon nanotube (MWNT) concrete. More than 100 cylindrical specimens were used to assess test variables during sensitivity observations, including water-cement ratios (0.75, 0.5, and 0.4) and exposure to chemical agents (including gum arabic, propanol, ethanol, sodium polyacrylate, methylcellulose, sodium dodecyl sulfate, and silane). To determine the adequate sonication time for MWNT dispersal in water, the compressive strengths of MWNT concrete cylinders were measured after sonication times ranging from 2 to 24 minutes. The results demonstrated that the addition of MWNT can increase the compressive strength of concrete by up to 108%. However, without chemical treatment, MWNT concretes tend to have poor freeze-thaw resistance. Among the different chemical treatments, MWNT concrete treated with sodium polyacrylate has the best compressive strength, chloride resistance, and freeze-thaw durability.

The exponential synchronization problem is investigated for a class of moving agent networks in a two-dimensional space and exhibits time-varying topology structure. Based on the Lyapunov stability theory, adaptive feedback controllers are developed to guarantee the exponential synchronization between each agent node. New criteria are proposed for verifying the locally and globally exponential synchronization of moving agent networks under the constraint of fast switching. In addition, a numerical example, including typical moving agent network with the Rössler system at each agent node, is provided to demonstrate the effectiveness and applicability of the proposed design approach.

Cleft lip with or without palate (CL/P) is a common congenital anomaly with a high birth prevalence in China. Based on a previous linkage signal of nonsyndromic CL/P (NSCL/P) on the chromosomal region 4q31-q32 from the Chinese populations, we screened the 4q31-q32 region for susceptibility genes in 214 trios of Han Chinese. PDGF-C, an important developmental factor, resides in the region and has been implicated in NSCL/P. However, in our family-based association test (transmission disequilibrium test; TDT), we could not conclude an association between PDGF-C and NSCL/P as previously suggested. Instead, we found strong evidence for parent-of-origin effect at a PDGF-C SNP, rs17035464, by a likelihood ratio test (unadjusted p-value = 0.0018; I(m) = 2.46). The location of rs17035464 is 13 kb downstream of a previously reported, NSCL/P-associated SNP, rs28999109. Furthermore, a patient from our sample trios was observed with a maternal segmental uniparental isodisomy (UPD) in a region containing rs17035464. Our findings support the involvement of PDGF-C in the development of oral clefts; moreover, the UPD case report contributes to the collective knowledge of rare variants in the human genome.