51. NLRP3 tyrosine phosphorylation is controlled by protein tyrosine phosphatase PTPN22
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Spalinger, Marianne R., Kasper, Stephanie, Gottier, Claudia, Lang, Silvia, Atrott, Kirstin, Vavricka, Stephan R., Scharl, Sylvie, Gutte, Petrus M., Grutter, Markus G., Beer, Hans-Dietmar, Contassot, Emmanuel, Chan, Andrew C., Dai, Xuezhi, Rawlings, David J., Mair, Florian, Becher, Burkhard, Falk, Werner, Fried, Michael, Rogler, Gerhard, and Scharl, Michael
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Tyrosine -- Research ,Phosphorylation -- Analysis ,Health care industry - Abstract
Inflammasomes form as the result of the intracellular presence of danger-associated molecular patterns and mediate the release of active IL-1β, which influences a variety of inflammatory responses. Excessive inflammasome activation results in severe inflammatory conditions, but physiological IL-1β secretion is necessary for intestinal homeostasis. Here, we have described a mechanism of NLRP3 inflammasome regulation by tyrosine phosphorylation of NLRP3 at Tyr861. We demonstrated that protein tyrosine phosphatase non-receptor 22 (PTPN22), variants in which are associated with chronic inflammatory disorders, dephosphorylates NLRP3 upon inflammasome induction, allowing efficient NLRP3 activation and subsequent IL-1β release. In murine models, PTPN22 deficiency resulted in pronounced colitis, increased NLRP3 phosphorylation, but reduced levels of mature IL-1β. Conversely, patients with inflammatory bowel disease (IBD) that carried an autoimmunity-associated PTPN22 variant had increased IL-1β levels. Together, our results identify tyrosine phosphorylation as an important regulatory mechanism for NLRP3 that prevents aberrant inflammasome activation., Introduction Inflammasomes are large, multiprotein complexes that form upon cytosolic or nuclear presence of damage- or pathogen-associated molecular patterns, ultimately leading to the secretion of IL-1β and IL-18. Inflammasomes consist [...]
- Published
- 2016
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