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55 results on '"T-Lymphocyte Subset"'

51. Differentiation of human NK cells into NK1 and NK2 subsets

Author

D. Peritt, S. Robertson, GRI, Giorgia, L. Showe, M. Aste Amezaga, G. Trinchieri, D. Peritt, S. Robertson, GRI, Giorgia, L. Showe, M. Aste Amezaga, and G. Trinchieri

Subjects
Cytotoxicity, Immunologic, cytology/immunology/secretion, Cells, Cytotoxicity, T-Lymphocyte Subset, Apoptosis, Antigens, CD95, biosynthesis, Apoptosis, immunology, Cell Differentiation, immunology, Cell Division, immunology, Cells, Cultured, Coculture Techniques, Cytokines, secretion, Cytotoxicity, Immunologic, Humans, Immunologic Memory, Immunophenotyping, Interleukin-12, pharmacology, Killer Cells, Natural, cytology/immunology/metabolism, Lymphocyte Subsets, cytology/immunology/metabolism, T-Lymphocyte Subsets, cytology/immunology/secretion, Th1 Cells, cytology/metabolism, Th2 Cells, cytology/metabolism, Immunophenotyping, immunology, biosynthesi, Th2 Cells, Immunologic, T-Lymphocyte Subsets, Humans, Killer Cells, Killer Cell, fas Receptor, Coculture Technique, Th2 Cell, Cytokine, Cells, Cultured, Cultured, Apoptosi, Cell Differentiation, cytology/immunology/metabolism, Th1 Cells, Interleukin-12, Coculture Techniques, Lymphocyte Subsets, Killer Cells, Natural, secretion, Th1 Cell, Antigen, Lymphocyte Subset, Cytokines, Cell, biosynthesis, pharmacology, Immunologic Memory, Cell Division, Human
Abstract

Human NK cells cultured in the presence of IL-12 or IL-4 differentiate into cell populations with distinct patterns of cytokine secretion similar to Th1 and Th2 cells. NK cells grown in IL-12 (NK1) produce IL-10 and IFN-gamma, whereas NK cells grown in IL-4 (NK2) produce IL-5 and IL-13. Although these NK cell subsets do not differ in cytotoxic activity, NK1 cells express higher levels of cell surface CD95 (Fas) Ag than NK2 cells and are more sensitive to Ab or chemically induced apoptosis. Like Th1 cells, NK1 cells accumulate much higher levels of the IL-12Rbeta2-chain mRNA and are significantly more responsive to IL-12 than NK2 cells at the level of activation of STAT4 transcription factor. The identification of NK cell subsets that are analogous to T cell subsets suggests a new role for NK cells in innate inflammatory responses and in their effect on adaptive immunity.

Published
1998

52. [Brain derived neurotrophic factor enhances the role of mesenchymal stem cells in inhibiting follicular helper T cells].

Author

Yang SN, Pu X, Xiang SL, Chen JP, and Pei L

Subjects
Brain-Derived Neurotrophic Factor, Cell Differentiation, Flow Cytometry, Humans, T-Lymphocytes, Helper-Inducer, Mesenchymal Stem Cells
Abstract

Objective: To investigate the effect of brain derived neurotrophic factor (BDNF) on mesenchymal stem cells (MSC) inhibiting follicular helper T cells (Tfh cells). Methods: The contents of indoleamine 2,3-dioxygenase (IDO), IL-10, TGF-β and IL-21 in MSC culture supernatant were detected by ELISA; The peripheral blood of healthy volunteers were collected, and lymphocyte in peripheral blood was separated by human lymphocyte separation solution; Co-cultures of MSC and lymphocyte were performed by Transwell chamber, and the proportion of CD4(+)CXCR5(+) Tfh cells and their subtypes were detected by flow cytometry. Results: ①The concentrations of IL-10, TGF-β, and IDO in the supernatant of BDNF group (BDNF-stimulated MSC) were higher than those of the control ones (adding PBS with the same volume) [IL-10: (42.1±4.4) ng/ml vs (19.3±2.1) ng/ml, t =4.761, P =0.009; TGF-β: (13.9±1.7) ng/ml vs (5.3±0.6) ng/ml, t =5.129, P =0.008; IDO: (441.3±56.9) ng/ml vs (226.7±37.6) ng/ml, t =3.130, P =0.035]; ②The comparisons between BDNF (co-culture of lymphocyte and BDNF-stimulated MSC) and MSC groups (co-culture of lymphocyte and MSC) were detailed as of follows: the proportion of CD4(+) CXCR5(+)Tfh cells were lower [(3.37±0.21)% vs (6.51±0.27)%, t =9.353, P <0.001], the proportion of CD4(+) CXCR5(+)CXCR3(+) CCR6(-) Tfh cells were higher [(41.14±2.04)% vs (26.72±2.57)%, t =4.383, P =0.012], CD4(+)CXCR5(+)CXCR3(-)CCR6(-) Tfh2 cells and CD4(+)CXCR5(+)CXCR3(-)CCR6(+) Tfh17 cells were lower [Tfh2: (30.16±5.38)% vs (43.26±4.11)%, t =4.426, P =0.012; Tfh17: (15.61±1.52)% vs (22.32±0.72)%, t =4.202, P =0.014], the proportion of CD4(+)CXCR5(+)Foxp3(+) Tfr cells were higher [(4.95±0.22)% vs (2.32±0.16)%, t =10.241, P <0.001], the concentration of IL-21 in the lymphocyte supernatant was lower [(0.28±0.03) ng/ml vs (0.85±0.08) ng/ml, t =6.675, P =0.003]. Conclusion: BDNF could enhance the inhibitory effect of MSC on Tfh cells through inhibiting the increasing of Tfh cells and the differentiations of Tfh2 and Tfh17 cells.

Published
2018
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53. Ig-specific T cell receptor-transgenic T cells are not deleted in the thymus and are functional in vivo

Author

Granucci, F, Rescigno, M, Marconi, G, Foti, M, Castagnoli, P, GRANUCCI, FRANCESCA, FOTI, MARIA, CASTAGNOLI, PAOLA, Granucci, F, Rescigno, M, Marconi, G, Foti, M, Castagnoli, P, GRANUCCI, FRANCESCA, FOTI, MARIA, and CASTAGNOLI, PAOLA

Abstract

The mechanisms that induce T cell tolerance to circulating self-proteins are still controversial, and both the deletion and selection of autoreactive T cells have been observed in the thymus of transgenic mouse models. To address the question of the induction of tolerance to circulating self-constituents, a T cell receptor-transgenic mouse specific for the serum protein immunoglobulin (Ig) gamma and (IgG2ab) was generated. The choice of an allotype-specific T cell also allowed the generation of transgenic control mice not expressing the self-antigen. It was found that the transgenic T cells were not deleted in the thymus, did not become tolerant in the periphery, and regulated the function of gamma 2ab-positive B cells as shown by the lack of IgG2ab protein in the serum of the transgenic mice. In spite of this activity in vivo, the transgenic T cells did not proliferate in vitro in response to the allotype-specific peptide. Interestingly, antigen-specific T cell proliferation could be restored if the transgenic mice were previously challenged to induce IgG2ab responses. After this challenge, IgG2ab protein in the serum of the transgenic mice could be partially restored, although still remaining much lower than in control mice. In addition, there was a dramatic increase in serum IgE levels, suggesting that newly generated gamma 2ab-secreting B cells can be induced to switch to IgE in the presence of allotype-specific T cells. These results indicate that Ig-specific T cells may represent a late-acting form of T cell help for the regulation of the IgG2a-to-IgE class switch

Published
1996

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