159 results on '"Sun, YR"'
Search Results
52. Dispersion-like lineshape observed in cavity-enhanced saturation spectroscopy of HD at 1.4 µm.
- Author
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Hua TP, Sun YR, and Hu SM
- Abstract
Precision measurement of ro-vibrational transitions in the electronic ground state of the hydrogen molecule can be used to test quantum electrodynamics and also to determine the dimensionless proton-to-electron mass ratio. Saturation spectroscopy of the 2-0 overtone transitions of hydrogen deuterium (HD) were measured with three cavity-enhanced spectroscopy methods. With a sensitivity at the 10
-13 c m-1 level, we revealed a dispersion-like lineshape instead of a conventional Lamb "dip," which explains the significant discrepancy among previous independent measurements. The spectra can be fit well by using the Fano profile. Centers of R(1) and R(3) lines were determined as 217 105 182 111 (19)s t a t (240)s y s k H z and 220 704 305 234 (20)s t a t (240)s y s k H z , respectively.- Published
- 2020
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53. Precision spectroscopy of atomic helium.
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Sun YR and Hu SM
- Abstract
Helium is a prototype three-body system and has long been a model system for developing quantum mechanics theory and computational methods. The fine-structure splitting in the 2
3 P state of helium is considered to be the most suitable for determining the fine-structure constant α in atoms. After more than 50 years of efforts by many theorists and experimentalists, we are now working toward a determination of α with an accuracy of a few parts per billion, which can be compared to the results obtained by entirely different methods to verify the self-consistency of quantum electrodynamics. Moreover, the precision spectroscopy of helium allows determination of the nuclear charge radius, and it is expected to help resolve the 'proton radius puzzle'. In this review, we introduce the latest developments in the precision spectroscopy of the helium atom, especially the discrepancies among theoretical and experimental results, and give an outlook on future progress., (© The Author(s) 2020. Published by Oxford University Press on behalf of China Science Publishing & Media Ltd.)- Published
- 2020
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54. [Primary development of visual uroflow scale].
- Author
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Zhang WY, Wang HR, Liu XH, Wang T, Chen JW, Sun YR, Zhang XP, Hu H, and Xu KX
- Subjects
- Cohort Studies, Humans, Lower Urinary Tract Symptoms, Male, Urination, Urodynamics
- Abstract
Objective: To develop the visual uroflow scale (VUS), analyze the relationship of VUS score and index of free uroflowmetry, assess urination function preliminarily and improve the work efficiency in the clinic., Methods: Male lower urinary tract symptoms (LUTS) patients, who attended the Department of Urology in Peking University People's Hospital from March 2016 to March 2017, were assessed for their urination function according to the Visual Uroflow Scale without help from clinicians before undertaking a free uroflowmetry test. And afterwards, a free uroflowmetry was undertaken, and variables including maximal flow rate (Qmax), the average flow rate (Qave) and voiding volume (VV) was obtained. During the study, 124 cases were collected and 53 cases met the inclusion and exclusion criteria and were included in the study cohort. The Spearman correlation analysis was used for analyzing the correlation of VUS scores with free uroflowmetry variables and age. The validity of VUS was evaluated., Results: Most of the patients could choose the very figure matched with self-condition by first instinct without any help from the clinician. The data were analyzed by Spearman correlation analysis. In the present study, voiding time was positively correlated with the VUS score (correlation coefficient, 0.62, P < 0.05). In the present cohort, the patients chose the third and fourth figures to take longer time to urinate, implying worse LUTS situation. Flow time and VUS scores were positively correlated (correlation coefficient, 0.61, P < 0.05). The patients with higher VUS scores would spend more time on urinate, no matter how long urinary hesitation was. Both Qmax and Qave were negatively correlated with the VUS score (correlation coefficient -0.54, -0.62, P < 0.05). The study illustrated that the VUS score suggested that the Qmax basically and further reflected the urination function. And its relationship to age revealed the decreased urination function of aging male, which had reached a consensus., Conclusion: Development of VUS has helped the clinician assess the urination function preliminarily at the first time. Patients are assessed for a VUS score before getting surgery or receiving the drug for treatment, and can be re-assessed after. The VUS score can provide an objective quantitative basis to evaluate the treatment efficacy. In addition, considering that it is convenient, timesaving and easy to understand, the VUS is available for follow-up.
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- 2020
55. Circulating neutrophil-to-lymphocyte ratio at admission predicts the long-term outcome in acute traumatic cervical spinal cord injury patients.
- Author
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Zhao JL, Lai ST, Du ZY, Xu J, Sun YR, Yuan Q, Wu X, Li ZQ, Hu J, and Xie R
- Subjects
- China, Humans, Infant, Newborn, Lymphocytes, Neutrophils, Retrospective Studies, Cervical Cord, Spinal Cord Injuries diagnosis
- Abstract
Background: The prognostic value of Neutrophil-to-Lymphocyte Ratio (NLR) for the outcome of acute cervical traumatic spinal cord injury (tSCI) patients has rarely been studied by now throughout the world., Methods: We performed a single-center retrospective cohort study to evaluate the prognostic value of NLR from peripheral whole blood count in patients with acute cervical tSCI. Patients within 6 h of acute cervical tSCI treated between Dec 2008 and May 2018 in Huashan Hospital of Fudan University were enrolled. Outcomes of patients with tSCI were assessed using American spinal injury association Impairment Scale (AIS). 6-month outcomes were dichotomized into poor outcome group (AIS A to C) and good outcome group (AIS D and E). Uni- and multivariate analyses were performed to assess the independent predictors of 6-month outcome. Two prediction models based on admission characteristics were built to evaluate the prognostic value of NLR. The discriminative ability of predictive models was evaluated using the area under the curve (AUC)., Results: A total of 377 patients were identified from our single center in China PR. Multivariate analysis showed that age, AIS grade at admission, NLR (p < 0.001) and coagulopathy (p = 0.003) were independent predictors of the 6-months outcome for acute cervical tSCI patients. The model combing NLR and standard variables (AUC = 0.944; 95% CI, 0.923-0.964) showed a more favorable prognostic value than that without NLR (AUC = 0.841; 95% CI, 0.798-0.885) in terms of 6-month outcome., Conclusions: NLR is firstly identified as an independent predictor of the 6-month outcome in acute cervical tSCI patients worldwide. The prognostic value of NLR is favorable, and a high NLR is associated with poor outcome in patients with acute cervical tSCI.
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- 2020
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56. ISO-alpha-acids improve the hematoma resolution and prevent peri-hematoma inflammations by transforming microglia via PPARgamma-CD36 axis in ICH rats.
- Author
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Zhao JL, Chen YJ, Yu J, Du ZY, Yuan Q, Sun YR, Wu X, Li ZQ, Wu XH, Hu J, and Xie R
- Subjects
- Animals, Anti-Inflammatory Agents therapeutic use, CD36 Antigens genetics, CD36 Antigens metabolism, Cell Differentiation, Cytokines metabolism, Disease Models, Animal, Humans, Humulus immunology, Indoleacetic Acids pharmacology, PPAR gamma agonists, Rats, Rats, Sprague-Dawley, Signal Transduction, Th2 Cells immunology, Up-Regulation, Brain Edema drug therapy, Cerebral Hemorrhage drug therapy, Hematoma drug therapy, Indoleacetic Acids therapeutic use, Microglia immunology, Plant Extracts therapeutic use
- Abstract
Objective: To elucidate the effects of ISO-α-acids (IAAs), a PPAR-γ agonist, on ICH rats and its potential mechanism., Material and Methods: The Sprague Dawley rats ICH model was induced by stereotactic injecting of 100 μl autologous artery blood. Ninety male rats were randomly allocated to five groups: autologous blood and IAAs (IAA); received autologous blood, IAAs and PPAR-γ inhibitor (IAA + GW9662); autologous blood and normal Saline (Saline); only autologous blood (Mock); and only needle injection (Sham). Neurological functions were assessed by mNSS. Hematoma volume, brain water content, surface proteins and inflammatory factors were detected. The microglia anti-inflammatory abilities were also evaluated., Results: IAAs were able to significantly decrease ICH rat's mNSS scores, alleviate brain water content, improve hematoma resolution than Saline, Mock (p < 0.05). More "M2" microglial/macrophage can be induced by IAAs. The expression of CD 36 was statistically higher in IAA than other groups (p < 0.05). Injection of IAAs led to a greatly increasing in CD 11b and CD 206 double-positive anti-inflammatory type microglial/macrophage, moreover, a reduction of inflammatory cytokines expression (p < 0.05). Such protective effects can be relieved by GW9662., Conclusions: This is the first study to elucidate the relationship between IAAs and ICH. IAAs were able to accelerate hematoma absorption, alleviate brain edema, suppress peri-hematoma inflammations and finally improved the outcome of ICH rats. The phenotype was due to the IAAs induction of "M2" microglial/macrophage via activating of PPAR-γ and increasing CD 36 expression., Competing Interests: Declaration of Competing Interest The authors declared that there is no conflict of interest., (Copyright © 2020 Elsevier B.V. All rights reserved.)
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- 2020
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57. Evidence-based umbrella review of 162 peripheral biomarkers for major mental disorders.
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Carvalho AF, Solmi M, Sanches M, Machado MO, Stubbs B, Ajnakina O, Sherman C, Sun YR, Liu CS, Brunoni AR, Pigato G, Fernandes BS, Bortolato B, Husain MI, Dragioti E, Firth J, Cosco TD, Maes M, Berk M, Lanctôt KL, Vieta E, Pizzagalli DA, Smith L, Fusar-Poli P, Kurdyak PA, Fornaro M, Rehm J, and Herrmann N
- Subjects
- Biomarkers, Humans, Autism Spectrum Disorder, Bipolar Disorder diagnosis, Depressive Disorder, Major, Mental Disorders, Schizophrenia
- Abstract
The literature on non-genetic peripheral biomarkers for major mental disorders is broad, with conflicting results. An umbrella review of meta-analyses of non-genetic peripheral biomarkers for Alzheimer's disease, autism spectrum disorder, bipolar disorder (BD), major depressive disorder, and schizophrenia, including first-episode psychosis. We included meta-analyses that compared alterations in peripheral biomarkers between participants with mental disorders to controls (i.e., between-group meta-analyses) and that assessed biomarkers after treatment (i.e., within-group meta-analyses). Evidence for association was hierarchically graded using a priori defined criteria against several biases. The Assessment of Multiple Systematic Reviews (AMSTAR) instrument was used to investigate study quality. 1161 references were screened. 110 met inclusion criteria, relating to 359 meta-analytic estimates and 733,316 measurements, on 162 different biomarkers. Only two estimates met a priori defined criteria for convincing evidence (elevated awakening cortisol levels in euthymic BD participants relative to controls and decreased pyridoxal levels in participants with schizophrenia relative to controls). Of 42 estimates which met criteria for highly suggestive evidence only five biomarker aberrations occurred in more than one disorder. Only 15 meta-analyses had a power >0.8 to detect a small effect size, and most (81.9%) meta-analyses had high heterogeneity. Although some associations met criteria for either convincing or highly suggestive evidence, overall the vast literature of peripheral biomarkers for major mental disorders is affected by bias and is underpowered. No convincing evidence supported the existence of a trans-diagnostic biomarker. Adequately powered and methodologically sound future large collaborative studies are warranted.
- Published
- 2020
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58. [Clinical characteristics and gene expression profiles in children with ETV6-RUNX1 acute lymphoblastic leukemia].
- Author
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Zheng XL, Wang ZY, Sun YR, Zhang H, Gao C, Zhang RD, Liu Y, Peng YG, Han JDJ, and Zheng HY
- Subjects
- Child, Core Binding Factor Alpha 2 Subunit, Humans, Neoplasm, Residual, Oncogene Proteins, Fusion, Transcriptome, Precursor Cell Lymphoblastic Leukemia-Lymphoma
- Abstract
Objective: To evaluate the heterogeneity in pediatric ETV6-RUNX1 acute lymphoblastic leukemia (ALL) by gene expression profile and to study clinical characteristics in different clusters. Methods: An improved advanced fragment analysis (iAFA) technique was developed to detect 57 marker genes in 264 pediatric ALL patients treated in Beijing Children's Hospital from August 2016 to June 2019. The 56 ALL patients with ETV6-RUNX1 positive were evaluated by clinical characteristics in gene expression profile, immunophenotype and early response of chemotherapy in different clusters. Results: The 56 ETV6-RUNX1-positive patients were clustered into 2 groups of E/R-1 (45, 80.4%) and E/R-2 (11, 19.6%) . Spearman coefficient was 0.788 and 0.901 in E/R-2 and E/R-1, respectively. The median of initial platelet counts was 104 (27-644) and 50 (8-390) ( P <0.01) in E/R-2 and E/R-1, respectively. The median of proportion of initial bone marrow immature cells was 0.830 (0.270-0.975) and 0.935 (0.445-0.990) ( P <0.05) in E/R-2 and E/R-1, respectively. The most specific immunophenotype at initial diagnosis, CD22(+)CD34(+)CD20(-)CD117(-)CD56(-), mainly gathered in E/R-2 ( P <0.001) . Patients negative of minimal residual disease detected by flow cytometry (MRD-FCM) at day 33 were 5 (55.6%) and 32 (88.9%) in E/R-2 and E/R-1, respectively. There was no significant difference in the original analysis ( P =0.064) but difference in sensitivity analysis ( P =0.035) . Nevertheless, patients negative of MRD detected by polymerase chain reaction (MRD-PCR) at day 33 were 7 (77.8%) and 36 (100%) in E/R-2 and E/R-1, respectively, with significant difference ( P =0.047) . Conclusion: Gene expression profile shows heterogeneous in ETV6-RUNX1 ALL, and the E/R-2 profile indicates that these patients may have a less tendency to thrombocytopenia at the initial diagnosis but have poorer response to induction chemotherapy and may influence further outcome.
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- 2020
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59. [Functions and applications of Multi-Tower Platform of Qingyuan Forest Ecosystem Research Station of Chinese Academy of Sciences].
- Author
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Gao T, Yu LZ, Yu FY, Wang XC, Yang K, Lu DL, Li XF, Yan QL, Sun YR, Liu LF, Xu S, Zhen XJ, Ni ZD, Zhang JX, Wang GF, Wei XH, Zhou XH, and Zhu JJ
- Subjects
- Atmosphere, Forests, Reproducibility of Results, Ecosystem, Trees
- Abstract
The relationship between the structure and function of forest ecosystems is the main intere-sts in the research area of forest ecology and management. However, over complex terrains in particular, these studies had been challenged as uneasy tasks due to the limitations in the forest survey and measurement techniques and other supporting technologies. Chinese Academy of Sciences (CAS) funded "Multi-Tower LiDAR/ECFlux Platform for Monitoring the Structure and Function of Secondary Forest Ecosystems" (Multi-Tower Platform, MTP) as a field station network corner-stone research infrastructure project, which was completed by Qingyuan Forest CERN (Chinese Ecosystem Research Network). In a distinctively-bounded and monitored-outlet watershed, the MTP was integrated by light detection and ranging (LiDAR) scanners, eddy covariance (EC) flux instrument systems, whole- and sub-watershed hydrology station network, long-term forest plot arrays, and live data center. Using LiDAR scanning, the MTP can get cloud data for holographic information on canopy structure. The EC-flux instrument system and hydrology station network along with forest plot arrays could ensure the reliability of water and carbon observations over this complex terrain, which allows to verify the studies on flux measurement technologies and methods, as well as to understand the processes of ecohydrology and CO
2 exchange between forest ecosystem and the atmosphere. Further, we can also assess the primary ecosystem services, including water conservation and carbon sequestration. All the data from "tower-station" were streamed through wireless network, which would facilitate data monitoring, management, and sharing. There are three tasks of MTP team: 1) defining innovative methods and descriptors to quantify three-dimensional forest structure; 2) developing theories and techniques to measure CO2 /H2 O fluxes and other trace gases over complex terrains; 3) understanding the relationship between structure and function of forest ecosystems, providing information and rationales for forest management practices to assure broad and sustainable benefits from forests.- Published
- 2020
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60. Seeded optical parametric oscillator light source for precision spectroscopy.
- Author
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Zhang ZT, Tan Y, Wang J, Cheng CF, Sun YR, Liu AW, and Hu SM
- Abstract
Precision spectroscopy of fundamental bands of molecules in the mid-infrared (MIR) region is of great interest in applications of trace detection and testing fundamental physics, where high-power and narrow-linewidth MIR lasers are needed. By using a frequency-stabilized near-infrared laser as a seed of the signal light of a continuous-wave optical parametric oscillator, we established a broadly tunable MIR light source that has an output power of several hundred milliwatts and a linewidth of a few tens of kilohertz. The MIR laser frequency drift was reduced to below 1 kHz by using an optical frequency comb to stabilize the frequency of the 1064 nm pumping laser. The performance of the light source was investigated and tested by measuring the saturated absorption spectroscopy of a few molecular transitions at 3.3 µm.
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- 2020
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61. A well-isolated vibrational state of CO 2 verified by near-infrared saturated spectroscopy with kHz accuracy.
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Wu H, Hu CL, Wang J, Sun YR, Tan Y, Liu AW, and Hu SM
- Abstract
Quantitative determination of atmospheric CO
2 concentration by remote sensing relies on accurate line parameters. Lamb dips of the lines up to J'' = 72 in the 30013-00001 band at 1605 nm were measured using a comb-locked cavity ring-down spectrometer, and the positions were determined with an accuracy of a few kHz. A simple effective Hamiltonian model can fit the rotational energies in the 30013 state ideally within the experimental accuracy, indicating that the vibrational state is well-isolated and can be regarded as free from perturbations. From a comparison between other bands using a similar analysis, we conclude that the transitions in the 30013-00001 band could be more suitable as reference lines for sensing applications with the potentially improved line parameter accuracy.- Published
- 2020
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62. Comb-locked cavity ring-down spectroscopy with variable temperature.
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Wu H, Stolarczyk N, Liu QH, Cheng CF, Hua TP, Sun YR, and Hu SM
- Abstract
Temperature dependence of molecular absorption line shape is important information for spectroscopic studies and applications. In this work, we report a comb-locked cavity ring-down spectrometer employing a cryogenic cooler to perform absorption spectroscopy measurements at temperatures between 40 K and 300 K. As a demonstration, we recorded the spectrum of the R(0) line in the (2-0) band of HD at 46 K. The temperature was also confirmed by the Doppler width of the HD line. Spectra of CH
4 near 1.394 μm were also recorded in a wide temperature range of 70-300 K. Lower-state energies of methane lines were analyzed by fitting these spectra, which can be directly compared with the HITRAN and TheoReTS databases. Considerable deviations were observed, indicating the need to investigate the assignments of the methane lines in this region.- Published
- 2019
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63. [Analysis of urodynamic study of female outpatients with lower urinary tract symptoms and follow-up of the patients with detrusor underactive].
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Zhang WY, Xia QX, Hu H, Chen JW, Sun YR, Xu KX, and Zhang XP
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- Aged, Female, Follow-Up Studies, Humans, Middle Aged, Outpatients, Quality of Life, Retrospective Studies, Lower Urinary Tract Symptoms, Urodynamics
- Abstract
Objective: To find out the prevalence of lower urinary tract symptoms (LUTS) and analyze the characteristics of the urodynamics diagnosis in female outpatients. To study the natural history of detrusor underactive (DU) by the followed up., Methods: A retrospective study of the female LUTS outpatients in Peking University People's Hospital from Jan. 2005 to Dec. 2015 was performed. The basic information and the urodynamic results of a total of 656 female outpatients were collected. The distribution of storage symptoms, voiding symptoms, complications and urodynamic diagnosis were analyzed. The symptoms and urodynamic results were compared among the groups, which were divided according to age, urodynamic diagnosis and diabetes mellitus. A follow-up of 163 female DU outpatients was performed, including the treatments and the American Urological Association symptoms scores (AUAss)., Results: Frequency (25.03%) is the most common symptom in female outpatients, followed by stress urinary incontinence (20.04%), urgency (19.97%), and difficulty of voiding (17.32%). Stress urinary incontinence (SUI) accounted for the first (36.04%) of the whole outpatients, followed by the DU (24.08%), and bladder outlet obstruction (17.58%). The patients aged 51-60 years occupied the peak of almost all the diagnosis. There was a higher proportion of the young female patients than that of the middle and old patients diagnosed with no abnormal after the urodynamic study. The first, strong, urge and maximum bladder capacity were significantly larger in DU patients with diabetes than without diabetes. Follow-up results of the DU patients showed there was no significantly difference of the AUAss scores in both the two groups before and after the follow-up, but the quality of life decreased significantly., Conclusion: Female LUTS outpatients showed a main complaint of storage symptoms. SUI ranked the first in female patients with LUTS. With the increase of age, bladder sensation and detrusor function decrease. In elderly patients, DU became the first ranked disease instead of SUI. Diabetes can affect the sensory function of bladder in patients with DU, and then increase the difficulty of voiding. The patients with DU, absent from treatment, experienced a lower quality of life.
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- 2019
64. MiR-223-3p inhibits proliferation and metastasis of oral squamous cell carcinoma by targeting SHOX2.
- Author
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Sun C, Liu XH, and Sun YR
- Subjects
- 3' Untranslated Regions, Carcinoma, Squamous Cell pathology, Cell Line, Tumor, Cell Movement genetics, Down-Regulation, Gene Expression Regulation, Neoplastic, Humans, Mouth Mucosa pathology, Mouth Neoplasms pathology, Neoplasm Metastasis, Transfection, Apoptosis genetics, Carcinoma, Squamous Cell genetics, Cell Proliferation genetics, Homeodomain Proteins genetics, MicroRNAs genetics, Mouth Neoplasms genetics
- Abstract
Objective: The aim of this study was to explore the role of microRNA-233-3p (miR-233-3p) in the development of oral squamous cell carcinoma (OSCC), and to elucidate the underlying mechanism., Patients and Methods: The expression of miR-233-3p in OSCC tissues and cell lines was detected by quantitative real-time polymerase chain reaction (qRT-PCR). The target of miR-233-3p was detected and evaluated by L-test and Western blot assays, respectively. Furthermore, the effects of miR-233-3p on cell proliferation, migration and apoptosis were discussed by cell counting kit-8 (CCK-8), scratch-wound and flow cytometry test., Results: MiR-233-3p was lowly expressed in OSCC tissues and cells. Short stature homeobox 2 (SHOX2) was predicted and verified as the downstream target gene of miR-233-3p. Inhibiting the expression of SHOX2 could significantly reduce the malignant behaviors of OSCC cells. The proliferation, migration and anti-apoptotic abilities of miR-233-3p overexpressed cells were obviously limited. However, the recovery of SHOX2 counteracted the beneficial effect of miR-233-3p., Conclusions: MiR-223-3p acted as a tumor suppressor gene in OSCC by targeting SHOX2. Our findings revealed that miR-223-3p/SHOX2 axis could be a potential therapeutic target for OSCC.
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- 2019
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65. Enterotype-based Analysis of Gut Microbiota along the Conventional Adenoma-Carcinoma Colorectal Cancer Pathway.
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Yang TW, Lee WH, Tu SJ, Huang WC, Chen HM, Sun TH, Tsai MC, Wang CC, Chen HY, Huang CC, Shiu BH, Yang TL, Huang HT, Chou YP, Chou CH, Huang YR, Sun YR, Liang C, Lin FM, Ho SY, Chen WL, Yang SF, Ueng KC, Huang HD, Huang CN, Jong YJ, and Lin CC
- Subjects
- Adenoma pathology, Aeromonas genetics, Aeromonas pathogenicity, Aged, Bacteroidaceae genetics, Bacteroidaceae pathogenicity, Colorectal Neoplasms pathology, Enterococcus genetics, Enterococcus pathogenicity, Escherichia genetics, Escherichia pathogenicity, Female, Fusobacterium genetics, Fusobacterium pathogenicity, Haemophilus genetics, Haemophilus pathogenicity, Humans, Male, Middle Aged, RNA, Ribosomal, 16S genetics, Adenoma microbiology, Colorectal Neoplasms microbiology, Gastrointestinal Microbiome
- Abstract
The dysbiosis of human gut microbiota is strongly associated with the development of colorectal cancer (CRC). The dysbiotic features of the transition from advanced polyp to early-stage CRC are largely unknown. We performed a 16S rRNA gene sequencing and enterotype-based gut microbiota analysis study. In addition to Bacteroides- and Prevotella-dominated enterotypes, we identified an Escherichia-dominated enterotype. We found that the dysbiotic features of CRC were dissimilar in overall samples and especially Escherichia-dominated enterotype. Besides a higher abundance of Fusobacterium, Enterococcus, and Aeromonas in all CRC faecal microbiota, we found that the most notable characteristic of CRC faecal microbiota was a decreased abundance of potential beneficial butyrate-producing bacteria. Notably, Oscillospira was depleted in the transition from advanced adenoma to stage 0 CRC, whereas Haemophilus was depleted in the transition from stage 0 to early-stage CRC. We further identified 7 different CAGs by analysing bacterial clusters. The abundance of microbiota in cluster 3 significantly increased in the CRC group, whereas that of cluster 5 decreased. The abundance of both cluster 5 and cluster 7 decreased in the Escherichia-dominated enterotype of the CRC group. We present the first enterotype-based faecal microbiota analysis. The gut microbiota of colorectal neoplasms can be influenced by its enterotype.
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- 2019
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66. Selection of Patients' Recumbent Position Laterality According to Physician Handedness Bias Increases the Success Rate of Lumbar Puncture: A Multicenter Study.
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Zhao JL, Sun YR, Shan Q, Xiao ZP, Hou BC, and Hu J
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- Adult, Female, Functional Laterality, Humans, Male, Middle Aged, Prospective Studies, Spinal Puncture adverse effects, Treatment Outcome, Young Adult, Neurosurgeons, Patient Positioning, Spinal Puncture methods
- Abstract
Background: Lumbar puncture (LP) is a medical procedure required during spinal anesthesia and for obtaining cerebrospinal fluid samples in the diagnosis of neurological disorders. The aim of this study was to assess the effects of physicians' handedness bias and the laterality of patients' recumbent position on the success rate of LPs., Methods and Patients: A prospective multicenter study including 36 physicians (18 left-handed and 18 right-handed) and 7200 patients was conducted in 6 medical centers. In each center, 1200 patients were randomized into group L (LPs performed by left-handed physicians) or group R (LPs performed by right-handed physicians). Each physician performed 200 cases of LPs, of which the laterality of the recumbent position (either on the left or right side) was decided after a second randomization. A successful LP was considered when the free flow of cerebrospinal fluid was observed upon the first attempt., Results: There was no significant difference in patient characteristics between groups L and R. Right-handed physicians had a significantly higher LP success rate with patients in the left lateral recumbent position (LRP) (1595/1800 vs. 1408/1800; odds ratio, 0.539; 95% confidence interval, 0.348-0.836; P=0.006). For left-handed physicians, the LP success rate was higher when patients were in the right LRP (1424/1800 vs. 1593/1800, odds ratio, 0.449; 95% confidence interval, 0.283-0.711; P=0.001). Patients' age, sex, height, and weight were not statistically related to LP success during multivariate analyses., Conclusions: Physicians handedness bias and patient laterality of recumbent position affects the success of LPs. Right-handed physicians have a greater chance of performing successful LPs when patients are in the left LRP, and vice versa.
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- 2019
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67. Interleukin-8 as a therapeutic target for chronic low back pain: Upregulation in human cerebrospinal fluid and pre-clinical validation with chronic reparixin in the SPARC-null mouse model.
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Krock E, Millecamps M, Anderson KM, Srivastava A, Reihsen TE, Hari P, Sun YR, Jang SH, Wilcox GL, Belani KG, Beebe DS, Ouellet J, Pinto MR, Kehl LJ, Haglund L, and Stone LS
- Subjects
- Adult, Animals, Cytokines metabolism, Disease Models, Animal, Drug Evaluation, Preclinical, Female, Humans, Interleukin-8 cerebrospinal fluid, Intervertebral Disc Degeneration complications, Intervertebral Disc Degeneration diagnosis, Low Back Pain diagnosis, Low Back Pain drug therapy, Magnetic Resonance Imaging, Male, Mice, Mice, Knockout, Middle Aged, Signal Transduction, Interleukin-8 metabolism, Low Back Pain etiology, Low Back Pain metabolism, Osteonectin deficiency, Sulfonamides pharmacology
- Abstract
Background: Low back pain (LBP) is the leading global cause of disability and is associated with intervertebral disc degeneration (DD) in some individuals. However, many adults have DD without LBP. Understanding why DD is painful in some and not others may unmask novel therapies for chronic LBP. The objectives of this study were to a) identify factors in human cerebrospinal fluid (CSF) associated with chronic LBP and b) examine their therapeutic utility in a proof-of-concept pre-clinical study., Methods: Pain-free human subjects without DD, pain-free human subjects with DD, and patients with chronic LBP linked to DD were recruited and lumbar MRIs, pain and disability levels were obtained. CSF was collected and analyzed by multiplex cytokine assay. Interleukin-8 (IL-8) expression was confirmed by ELISA in CSF and in intervertebral discs. The SPARC-null mouse model of progressive, age-dependent DD and chronic LBP was used for pre-clinical validation. Male SPARC-null and control mice received systemic Reparixin, a CXCR1/2 (receptors for IL-8 and murine analogues) inhibitor, for 8 weeks. Behavioral signs of axial discomfort and radiating pain were assessed. Following completion of the study, discs were excised and cultured, and conditioned media was evaluated with a protein array., Findings: IL-8 was elevated in CSF of chronic LBP patients with DD compared to pain-free subjects with or without DD. Chronic inhibition with reparixin alleviated low back pain behaviors and attenuated disc inflammation in SPARC-null mice., Interpretation: These studies suggest that the IL-8 signaling pathway is a viable therapy for chronic LBP. FUND: Supported by NIH, MMF, CIHR and FRQS., (Copyright © 2019. Published by Elsevier B.V.)
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- 2019
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68. Intensive blood pressure control reduces the risk of progressive hemorrhage in patients with acute hypertensive intracerebral hemorrhage: A retrospective observational study.
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Zhao JL, Du ZY, Sun YR, Yuan Q, Yu J, Wu X, Li ZQ, Wu XH, Xie R, and Hu J
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- Acute Disease, Adolescent, Adult, Aged, Aged, 80 and over, Blood Pressure physiology, Blood Pressure Determination methods, Cohort Studies, Female, Humans, Hypertension diagnosis, Intracranial Hemorrhage, Hypertensive diagnosis, Intracranial Hemorrhage, Hypertensive surgery, Male, Middle Aged, Retrospective Studies, Risk Factors, Treatment Outcome, Young Adult, Antihypertensive Agents administration & dosage, Blood Pressure drug effects, Disease Progression, Hypertension drug therapy, Intracranial Hemorrhage, Hypertensive prevention & control
- Abstract
Objective: To investigate the impact of intensive blood pressure control on progressive intracerebral hemorrhage and outcome in patients with high blood pressure and intracerebral hemorrhage., Patients and Methods: A retrospective study was conducted recruiting 659 patients with acute hemorrhagic stroke between Jan. 2012 and May 2018. Patients recruited before May 2015 were treated with a target systolic level of <180 mm Hg, while those recruited after May 2015 received intensive blood pressure control treatment with a target systolic level of <140 mm Hg within 1 h. Uni- and multi-variate analysis were conducted to illustrate the association between intensive blood pressure control and progressive intracerebral hemorrhage. Mortality, rates of operation, length of ICU stay, modified Rankin scores at 90 days, and the rate of serious adverse events were also compared between the two groups., Results: A total of 351 and 308 patients with acute hypertensive intracerebral hemorrhage were recruited before and after May 2015, respectively. Progressive intracerebral hemorrhage was identified among 111 out of 659 patients. Patients who received intensive blood pressure control showed a statistically lower rate of hematoma enlarging (43 of 308, 13.9% vs. 74 of 351, 21.1%, p = 0.018). The rates of operation and modified Rankin scores at 90 days were statistically lower with intensive blood control, while the mortality, length of ICU stay and rate of serious adverse events were similar between the two groups. Intensive BP control is an independent factor in predicting hematoma growing, with a more favorable discrimination (AUC = 0.889; 95%CI, 0.859-0.917) than other two models (AUC = 0.821; 95%CI, 0.791-0.852; and AUC = 0.635; 95%CI, 0.588-0.682)., Conclusion: Intensive blood pressure control reduce the risk of progressive intracerebral hemorrhage and improved functional outcomes in patients with acute hemorrhagic stroke., (Copyright © 2019 Elsevier B.V. All rights reserved.)
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- 2019
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69. Prognostic Value of Neutrophil-to-Lymphocyte Ratio in Predicting the 6-Month Outcome of Patients with Traumatic Brain Injury: A Retrospective Study.
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Zhao JL, Du ZY, Yuan Q, Yu J, Sun YR, Wu X, Li ZQ, Wu XH, and Hu J
- Abstract
Background: Peripheral white blood cells are regularly analyzed on admission for patients with traumatic brain injury (TBI). The prognostic value of the neutrophil-to-lymphocyte ratio (NLR) in predicting the 6-month outcome of patients with TBI is unclear., Methods: We designed a single-center retrospective cohort study. Patients admitted to Fudan University Huashan Hospital within 6 hours after TBI were identified between December 2004 and December 2017. The primary outcome was 6-month Glasgow Outcome Scale score. Independent predictors of 6-month outcome were assessed using uni- and multivariate analyses. Three models based on admission characteristics were built to evaluate the prognostic value of the NLR in predicting the outcome of patients with TBI. The discriminative ability of predictive models was evaluated by the area under the curve (AUC)., Results: A total of 1291 patients with TBI were included. Multivariate analysis showed age, Glasgow Coma Scale scores at admission, subdural hematoma, intraparenchymal hemorrhage, traumatic subarachnoid hemorrhage, NLR (P < 0.001), and coagulopathy (P = 0.028) were independent predictors of 6-month outcome. The model combining the NLR and standard variables (AUC = 0.936; 95% confidence interval [CI], 0.923-0.949) was more favorable in predicting 6-month outcome of patients with TBI than the model without the NLR (AUC = 0.901; 95% CI, 0.883-0.919) and the model based only on the NLR (AUC = 0.827; 95% CI, 0.802-0.852)., Conclusions: NLR is an independent prognostic factor of predicting 6-month outcome of patients with TBI. A high NLR in patients with TBI is associated with poor outcome. The prognostic value of the NLR in predicting 6-month outcome of patients with TBI is favorable., (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Published
- 2019
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70. LINC00961 suppresses cell proliferation and induces cell apoptosis in oral squamous cell carcinoma.
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Pan LN and Sun YR
- Subjects
- Animals, Apoptosis genetics, Cell Line, Tumor, Cell Proliferation genetics, Disease Progression, Down-Regulation, Genes, Tumor Suppressor, Humans, Mice, Mouth Mucosa pathology, Mouth Mucosa surgery, Mouth Neoplasms pathology, Mouth Neoplasms surgery, Phosphatidylinositol 3-Kinases, Proto-Oncogene Proteins c-akt, Signal Transduction genetics, Squamous Cell Carcinoma of Head and Neck pathology, Squamous Cell Carcinoma of Head and Neck surgery, Xenograft Model Antitumor Assays, Gene Expression Regulation, Neoplastic, Mouth Neoplasms genetics, Peptides metabolism, Squamous Cell Carcinoma of Head and Neck genetics
- Abstract
Objective: Oral squamous cell carcinoma (OSCC) is still one of the most frequent neck and head malignancies and is one of the most common cancers in the world. The main purpose of this research was to illustrate the functional role of LINC00961 in OSCC and provide novel insight of biomarkers and therapeutic strategies in OSCC., Patients and Methods: The relative expression level of LINC00961 was evaluated by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Cell counting kit-8 (CCK-8) assay was involved for determining the ability of cell proliferation. Flow cytometric analysis was performed to detect the cell cycle and cell apoptosis. Expressions of AKT, p-AKT, BCL2, Bax protein levels were detected in Western blotting. Transfected cells were used to perform tumor xenograft formation assay., Results: Low-expression of LINC00961 was detected in both OSCC tissues and cell lines. Through CCK-8 assay and flow cytometric analysis, we validated that up-regulated LINC00961 suppressed cell proliferation and promoted cell apoptosis in OSCC. Besides, over-expressed LINC00961 suppressed PI3K/AKT signaling pathways. In tumor xenograft formation assay, over-expressed LINC00961 inhibited tumor formation., Conclusions: Our research verified that LINC00961 functioned as a tumor suppressor in OSCC. Regulation of PI3K/AKT might be the underlying mechanism of the tumor suppressor role of LINC00961. The current study might bring a novel insight of biomarkers and therapeutic strategies in OSCC.
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- 2019
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71. Frequency metrology of molecules in the near-infrared by NICE-OHMS.
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Hua TP, Sun YR, Wang J, Liu AW, and Hu SM
- Abstract
Noise-immune cavity enhanced optical heterodyne molecular spectroscopy (NICE-OHMS) is extremely sensitive in detecting weak absorption. However, the use of NICE-OHMS for metrology study was also hindered by its sensitivity to influence from various experimental conditions such as the residual amplitude modulation. Here we demonstrate to use NICE-OHMS for precision measurements of Lamb-dip spectra of molecules. After a dedicated investigation of the systematic uncertainties in the NICE-OHMS measurement, the transition frequency of a ro-vibrational line of C
2 H2 near 789 nm was determined to be 379 639 280 915.3±1.2 kHz (fractional uncertainty 3.2 × 10-12 ), agreeing well with, but more accurate than, the value determined from previous cavity ring-down spectroscopy measurements. The study indicates the possibility to implement the very sensitive NICE-OHMS method for frequency metrology of molecules, or a molecular clock, in the near-infrared.- Published
- 2019
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72. Asymmetric controlled bidirectional remote preparation of two- and three-qubit equatorial state.
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Sun YR, Chen XB, Xu G, Yuan KG, and Yang YX
- Abstract
In this paper, a novel asymmetric controlled bidirectional remote preparation scheme is proposed. In our scheme, Alice and Bob are not only the senders but also the receivers with the control of Charlie. By using the eleven-qubit entangled state as the quantum channel, Alice prepares an arbitrary two-qubit equatorial state for Bob and Bob prepares an arbitrary three-qubit equatorial state for Alice simultaneously. Firstly, we give the construction process of the quantum channel. Secondly, the whole recovery operations are given. Alice and Bob can recover the prepared state determinately. Thirdly, we consider the effect of the noisy environment (amplitude-damping and phase-damping) in our scheme and calculate the fidelities of the output states. Finally, since our scheme does not need additional operations and auxiliary qubits, the efficiency of our scheme is higher than that of the previous schemes.
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- 2019
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73. Lithium Carbonate in a Poststroke Population: Exploratory Analyses of Neuroanatomical and Cognitive Outcomes.
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Sun YR, Herrmann N, Scott CJM, Black SE, Swartz RH, Hopyan J, and Lanctôt KL
- Subjects
- Aged, Antidepressive Agents adverse effects, Antidepressive Agents therapeutic use, Atrophy complications, Cognition Disorders complications, Female, Humans, Lithium Carbonate adverse effects, Magnetic Resonance Imaging, Male, Neuroimaging, Neurologic Examination, Neuropsychological Tests, Pilot Projects, Stroke complications, Stroke pathology, Treatment Outcome, Atrophy drug therapy, Cognition Disorders drug therapy, Gray Matter pathology, Lithium Carbonate therapeutic use, Memory drug effects, Stroke drug therapy
- Abstract
Purpose/background: Loss of gray matter after stroke has been associated with cognitive impairment. This pilot study aimed to investigate the therapeutic potential of lithium, a putative neurotrophic agent, in the stroke recovery process within a year of stroke occurrence., Methods: Twelve stroke patients (mean ± SD age, 71.1 ± 11.9 years) were recruited to the study, and eligible participants were prescribed open-label lithium for 60 days. Magnetic resonance imaging was used to assess global gray matter at baseline and end of treatment; global cognition was assessed using the standardized Mini-Mental State Examination and Montreal Cognitive Assessment, and verbal memory was evaluated using the Hopkins Verbal Learning Test-Revised., Findings/results: There was no difference in global gray matter volume between baseline and follow-up (t = 1.977, P = 0.074). There was a significant interaction between higher lithium dose and increased global gray matter volume (F = 14.25, P = 0.004) and a correlation between higher lithium dose and improved verbal memory (r = 0.576, P = 0.05)., Implications/conclusions: Lithium pharmacotherapy may be associated with gray matter volume change and verbal memory improvement in stroke patients, providing a rationale for future trials assessing therapeutic potential of lithium in a poststroke population.
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- 2019
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74. [Effects of soil salinity on Bt protein content and nitrogen metabolic physiology in boll shell of Bt cotton].
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Wang YH, Chen JP, Sun YR, Zhang X, and Chen H
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- Animals, Bacillus thuringiensis, Gossypium, Hemolysin Proteins, Insecta, Insecticides, Nitrogen, Plants, Genetically Modified, Bacterial Proteins metabolism, Salinity, Soil chemistry
- Abstract
In order to clarify the effects of soil salinity on the insect-resistance of boll in transgenic Bt cotton, potted plants of two Bt cotton cultivars Xinmian 33B (salt-sensitive) and Zhong 07 (salt-tolerant) were exposed to five levels of soil salinity (0, 0.15%, 0.30%, 0.45% and 0.60%). The results showed that Bt protein content of boll shell decreased with increasing soil salinity. Compared with the control (0% soil salinity level), the Bt protein content of boll shell decreased significantly when the soil salinity level was above 0.15% for Xinmian 33B and above 0.30% for Zhong 07. The reduction extent of Bt protein content of boll shell at 30 days post anthesis (DPA) was greater than that at 10 DPA under the same soil salinity level. Significant reductions of soluble protein contents, nitrate reductase (NR), and glutamate pyruvate transaminase (GPT) activities were observed when the boll shell Bt protein content was significantly reduced. The content of free amino acid, protease, and peptidase activity of boll shell significantly increased when the soil salinity level was above 0.30%. In conclusion, soil salinity affected boll shell nitrogen metabolism and reduced Bt protein synthesis. Middle and high soil salinity levels could enhance decomposition of Bt protein, which further decreases the expression level of insecticidal protein.
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- 2018
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75. Comparison of the toxic effects of different mycotoxins on porcine and mouse oocyte meiosis.
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Lu Y, Zhang Y, Liu JQ, Zou P, Jia L, Su YT, Sun YR, and Sun SC
- Abstract
Background: Aflatoxin B1 (AFB1), deoxynivalenol (DON), HT-2, ochratoxin A (OTA), zearalenone (ZEA) are the most common mycotoxins that are found in corn-based animal feed which have multiple toxic effects on animals and humans. Previous studies reported that these mycotoxins impaired mammalian oocyte quality. However, the effective concentrations of mycotoxins to animal oocytes were different., Methods: In this study we aimed to compare the sensitivity of mouse and porcine oocytes to AFB1, DON, HT-2, OTA, and ZEA for mycotoxin research. We adopted the polar body extrusion rate of mouse and porcine oocyte as the standard for the effects of mycotoxins on oocyte maturation., Results and Discussion: Our results showed that 10 μM AFB1 and 1 μM DON significantly affected porcine oocyte maturation compared with 50 μM AFB1 and 2 μM DON on mouse oocytes. However, 10 nM HT-2 significantly affected mouse oocyte maturation compared with 50 nM HT-2 on porcine oocytes. Moreover, 5 μM OTA and 10 μM ZEA significantly affected porcine oocyte maturation compared with 300 μM OTA and 50 μM ZEA on mouse oocytes. In summary, our results showed that porcine oocytes were more sensitive to AFB1, DON, OTA, and ZEA than mouse oocytes except HT-2 toxin., Competing Interests: Shao-Chen Sun is an Academic Editor for PeerJ. Yong-Teng Su and Yu-Rong Sun are employed by Jiangsu Aomai Bio-tech Company.
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- 2018
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76. Toward a Determination of the Proton-Electron Mass Ratio from the Lamb-Dip Measurement of HD.
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Tao LG, Liu AW, Pachucki K, Komasa J, Sun YR, Wang J, and Hu SM
- Abstract
Precision spectroscopy of the hydrogen molecule is a test ground of quantum electrodynamics (QED), and it may serve for the determination of fundamental constants. Using a comb-locked cavity ring-down spectrometer, for the first time, we observed the Lamb-dip spectrum of the R(1) line in the overtone of hydrogen deuteride (HD). The line position was determined to be 217 105 182.79±0.03_{stat}±0.08_{syst} MHz (δν/ν=4×10^{-10}), which is the most accurate rovibrational transition ever measured in the ground electronic state of molecular hydrogen. Moreover, from calculations including QED effects up to the order m_{e}α^{6}, we obtained predictions for this R(1) line as well as for the HD dissociation energy, which are less accurate but signaling the importance of the complete treatment of nonadiabatic effects. Provided that the theoretical calculation reaches the same accuracy, the present measurement will lead to a determination of the proton-to-electron mass ratio with a precision of 1.3 parts per billion.
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- 2018
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77. Cognitive Outcomes After Transcatheter Aortic Valve Implantation: A Metaanalysis.
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Khan MM, Herrmann N, Gallagher D, Gandell D, Fremes SE, Wijeysundera HC, Radhakrishnan S, Sun YR, and Lanctôt KL
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- Aged, 80 and over, Female, Humans, Male, Neuropsychological Tests statistics & numerical data, Risk Factors, Aortic Valve surgery, Aortic Valve Stenosis surgery, Cognition physiology, Transcatheter Aortic Valve Replacement
- Abstract
Objectives: To quantitatively summarize changes in cognitive performance in individuals with severe aortic stenosis undergoing transcatheter aortic valve implantation (TAVI)., Design: Metaanalysis., Participants: Individuals undergoing TAVI (N = 1,065 (48.5% male) from 18 studies, average age ≥80)., Measurements: The MEDLINE, EMBASE, and Cochrane Central databases were searched for original peer-reviewed reports assessing cognitive performance using standardized cognitive tests before and after TAVI. Data were extracted for cognitive scores before TAVI; perioperatively (within 7 days after TAVI); 1, 3, and 6 months after TAVI, and 12 to 34 months after TAVI (over the long term). Standardized mean differences (SMDs) were generated using random-effects models for changes in cognition at each time point. Metaregression analyses were conducted to assess the association between population and procedural characteristics and cognitive outcomes. Risk of bias was assessed., Results: There were no significant changes from baseline in perioperative cognitive performance (SMD = 0.05, 95% confidence interval (CI) = -0.08-0.18; z = 0.75, P = .46), although overall cognitive performance had improved significantly 1 month after TAVI (SMD = -0.33, 95% CI = -0.50 to -0.16; z = 3.83, P < .001). There were no differences in cognitive performance 3 and 6 months after TAVI or over the long term. Cognitive outcomes were not associated with any covariates in regression analyses., Conclusion: Cognitive performance is preserved after TAVI, suggesting TAVI is not detrimental to cognition., (© 2017, Copyright the Authors Journal compilation © 2017, The American Geriatrics Society.)
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- 2018
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78. Erratum: "Communication: Molecular near-infrared transitions determined with sub-kHz accuracy" [J. Chem. Phys. 147, 091103 (2017)].
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Wang J, Sun YR, Tao LG, Liu AW, and Hu SM
- Published
- 2018
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79. Prognostic value of coagulation tests for in-hospital mortality in patients with traumatic brain injury.
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Yuan Q, Yu J, Wu X, Sun YR, Li ZQ, Du ZY, Wu XH, and Hu J
- Subjects
- Blood Coagulation Disorders epidemiology, Blood Coagulation Disorders etiology, Brain Injuries, Traumatic complications, Brain Injuries, Traumatic diagnosis, Brain Injuries, Traumatic epidemiology, China epidemiology, Female, Glasgow Coma Scale, Hospital Mortality trends, Humans, Incidence, Male, Middle Aged, Prognosis, Retrospective Studies, Blood Coagulation physiology, Blood Coagulation Disorders blood, Blood Coagulation Tests methods, Brain Injuries, Traumatic blood
- Abstract
Background: Coagulopathy is commonly observed after traumatic brain injury (TBI). However, it is not known whether using the standard independent predictors in conjunction with coagulation tests would improve their prognostic value. We determined the incidence of TBI-associated coagulopathy in patients with isolated TBI (iTBI), evaluated the prognostic value of coagulation tests for in-hospital mortality, and tested their predictive power for in-hospital mortality in patients with iTBI., Methods: We conducted a retrospective, observational database study on 2319 consecutive patients with iTBI who attended the Huashan Hospital Department of the Neurosurgery Neurotrauma Center at Fudan University in China between December 2004 and June 2015. Two models based on the admission characteristics were developed: model A included predictors such as age, Glasgow Coma Scale (GCS) score, pupil reactivity, type of injury, and hemoglobin and glucose levels, while model B included the predictors from model A as well as coagulation test results. A total of 1643 patients enrolled between December 2004 and December 2011 were used to derive the prognostic models, and 676 patients enrolled between January 2012 and June 2015 were used to validate the models., Results: Overall, 18.6% (n = 432) of the patients developed coagulopathy after iTBI. The prevalence of acute traumatic coagulopathy is associated with the severity of brain injury. The percentage of platelet count <100 × 109/L, international normalized ratio (INR) > 1.25, the prothrombin time (PT) > 14 s, activated partial thromboplastin time (APTT) > 36 s, D-dimer >5 mg/L and fibrinogen (FIB) < 1.5 g/L was also closely related to the severity of brain injury, significance being found among three groups. Age, pupillary reactivity, GCS score, epidural hematoma (EDH), and glucose levels were independent prognostic factors for in-hospital mortality in model A, whereas age, pupillary reactivity, GCS score, EDH, glucose levels, INR >1.25, and APTT >36 s exhibited strong prognostic effects in model B. Discrimination and calibration were good for the development group in both prediction models. However, the external validation test showed that calibration was better in model B than in model A for patients from the validation population (Hosmer-Lemeshow test, p = 0.152 vs. p = 0.046, respectively)., Conclusions: Coagulation tests can improve the predictive power of the standard model for in-hospital mortality after TBI.
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- 2018
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80. Global grey matter volume in adult bipolar patients with and without lithium treatment: A meta-analysis.
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Sun YR, Herrmann N, Scott CJM, Black SE, Khan MM, and Lanctôt KL
- Subjects
- Adult, Diffusion Magnetic Resonance Imaging, Gray Matter drug effects, Humans, Organ Size, Prefrontal Cortex pathology, Bipolar Disorder drug therapy, Gray Matter pathology, Lithium therapeutic use
- Abstract
Objective: The goal of this meta-analysis was to quantitatively summarize the evidence available on the differences in grey matter volume between lithium-treated and lithium-free bipolar patients., Methods: A systematic search was conducted in Cochrane Central, Embase, MEDLINE, and PsycINFO databases for original peer-reviewed journal articles that reported on global grey matter volume in lithium-medicated and lithium-free bipolar patients. Standard mean difference and Hedges' g were used to calculate effect size in a random-effects model. Risk of publication bias was assessed using Egger's test and quality of evidence was assessed using standard criteria., Results: There were 15 studies with a total of 854 patients (368 lithium-medicated, 486 lithium-free) included in the meta-analysis. Global grey matter volume was significantly larger in lithium-treated bipolar patients compared to lithium-free patients (SMD: 0.17, 95% CI: 0.01-0.33; z = 2.11, p = 0.035). Additionally, there was a difference in global grey matter volume between groups in studies that employed semi-automated segmentation methods (SMD: 0.66, 95% CI: 0.01-1.31; z = 1.99, p = 0.047), but no significant difference in studies that used fully-automated segmentation. No publication bias was detected (bias coefficient = - 0.65, p = 0.46)., Limitations: Variability in imaging methods and lack of high-quality evidence limits the interpretation of the findings., Conclusions: Results suggest that lithium-treated patients have a greater global grey matter volume than those who were lithium-free. Further study of the relationship between lithium and grey matter volume may elucidate the therapeutic potential of lithium in conditions characterized by abnormal changes in brain structure., (Crown Copyright © 2017. Published by Elsevier B.V. All rights reserved.)
- Published
- 2018
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81. Measurement of the Frequency of the 2 ^{3}S-2 ^{3}P Transition of ^{4}He.
- Author
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Zheng X, Sun YR, Chen JJ, Jiang W, Pachucki K, and Hu SM
- Abstract
The 2 ^{3}S-2 ^{3}P transition of ^{4}He was measured by comb-linked laser spectroscopy using a transverse-cooled atomic beam. The centroid frequency was determined to be 276 736 495 600.0(1.4) kHz, with a fractional uncertainty of 5.1×10^{-12}. This value is not only more accurate but also differs by as much as -49.5 kHz (20σ) from the previous result given by [Cancio Pastor et al., Phys. Rev. Lett. 92, 023001 (2004)PRLTAO0031-900710.1103/PhysRevLett.92.023001; Cancio Pastor et al.Phys. Rev. Lett.97, 139903(E) (2006)10.1103/PhysRevLett.97.139903; Cancio Pastor et al.Phys. Rev. Lett.108, 143001 (2012)10.1103/PhysRevLett.108.143001]. In combination with ongoing theoretical calculations, this work may allow the most accurate determination of the nuclear charge radius of helium.
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- 2017
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82. [Comparison and discussion of different surgical methods used to treat vesicovaginal fistulas].
- Author
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Zhang WY, Hu H, Zhang XP, Sun YR, Wang HR, and Xu KX
- Subjects
- Adult, Female, Humans, Laparoscopy, Middle Aged, Retrospective Studies, Gynecologic Surgical Procedures methods, Vesicovaginal Fistula surgery
- Abstract
Objective: To compare the feasibility and prognosis of different surgical methods used for vesicovaginal fistulas and to explore the value of electrocoagulation treating small ones., Methods: The medical data of 19 patients who had undertaken transvaginal VVF repairs in Peking University People's Hospital between October 2008 and November 2016 were retrospectively collected. The follow-ups were performed. The patients' age ranged from 31 to 55 years with the median age of 48 years and the history length ranged from 1 month to 24 months with the median length of 3 months. Their fistula situation, surgical methods and prognosis were analyzed and the differences and similarities were compared., Results: Three patients (15.79%) was performed by electrocoagulation, 4 (21.05%) by transvaginal repair, 5 (21.32%) by laparoscopic repair and 7(36.84%) by open operation. Except one patient who rejected urinary catheter and D-J catheters, the rest of the patients discharged with catheters. Twelve patients (63.2%) got full satisfaction with one operation. One of the 3 patients who undertook electrocoagulation repeated the operation for twice and got completely cured within 1 month while the other two undertook the operation once and got dry within 1 month. Three patients who undertook transvaginal repair got dry within 1 month. Two of the 5 patients who undertook laparoscopic repair had readmission for a second operation and the other 3 got dry after operation. Five of the 7 open repair patients got dry while the other 2 attempted other center for treatment., Conclusion: Transvaginal repair has been the main surgery procedure for VVF, but it is limited by the location of fistula and the condition of vaginal. For patients not suitable for transvaginal repair, laparoscopic repair and open surgery are feasible. However both laparoscopic repair and open surgery are more invasive. Based on that, electrocoagulation becomes a better choice. In our research, patients with small and high location fistula treated by electrocoagulation got a higher cure rate and bear less surgical trauma. Electrocoagulation used in the treatment of VVF showed advantages of less trauma, less bleeding and better satisfaction. Fistulas with low location were more suitable for transvaginal repair. Complex VVF, especially with narrow ureteral open and ureteral fistulas, were more suitable for open and laparoscopic repair. As for single and small fistula, the electrocoagulation can be the first choice.
- Published
- 2017
83. Effects of Deferoxamine Mesylate on Hematoma and Perihematoma Edema after Traumatic Intracerebral Hemorrhage.
- Author
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Yu J, Yuan Q, Sun YR, Wu X, Du ZY, Li ZQ, Wu XH, Zhou LF, Wu G, and Hu J
- Subjects
- Adult, Aged, Brain Edema etiology, Cerebral Hemorrhage, Traumatic complications, Female, Humans, Male, Middle Aged, Treatment Outcome, Brain Edema drug therapy, Cerebral Hemorrhage, Traumatic drug therapy, Deferoxamine therapeutic use, Siderophores therapeutic use
- Abstract
Deferoxamine mesylate can cross the blood-brain barrier and reduce iron accumulation in nervous tissue; moreover, it has a variety of neuroprotective functions in addition to complexing with iron ions. Such iron chelators are expected to become a new treatment option for intracerebral hemorrhage. This study evaluated the effects of deferoxamine mesylate on hematoma and edema absorption after traumatic intracerebral hemorrhage (TICH), and it provides clinical evidence for TICH treatment with deferoxamine mesylate. Patients with isolated TICH, confirmed by head computed tomography, were enrolled prospectively from January 2013 to December 2016. Patients were divided non-randomly into an experimental or control group as decided by the attending neurosurgeon. Patients in the experimental group received intravenous deferoxamine mesylate (20 mg/kg daily) from the day of admission for 5 consecutive days. We evaluated the impact of deferoxamine mesylate on the change in edema volume and the absorption of hematoma volume using a propensity score-matched analysis. In total, 190 patients were included. After matching, 94 patients were included in the final analysis (47 per group); no variable differed significantly between the two groups. The hematoma volume on the 7th day in the control group was higher than that at the same time-point in the experimental group (9.4 ± 7.2 vs. 5.2 ± 4.8 mL; p = 0.001). There was no difference in hematoma volume on Day 1 (12.6 ± 7.8 vs. 12.8 ± 6.4 mL; p = 0.896), Day 3 (12.4 ± 7.4 vs. 11.4 ± 4.9 mL; p = 0.442), and Day 14 (3.2 ± 3.0 vs. 2.5 ± 2.6 mL; p = 0.215) between the groups. The absorption of hematoma volume between the 1st and 3rd days and the 1st and 7th days in the experimental group was higher than that during the same periods in the control group. The edema volumes on the 3rd, 7th, and 14th days in the control group were higher than those at the same time-points in the experimental group. There was no difference in edema volume on the 1st day. The changes in edema volume between the 1st and 3rd days, the 1st and 7th days, and the 1st and 14th days in the control group were higher than those during the same periods in the experimental group. Deferoxamine mesylate may accelerate hematoma absorption and inhibit edema after TICH; however, further investigation is required to reach definitive conclusions.
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- 2017
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84. Communication: Molecular near-infrared transitions determined with sub-kHz accuracy.
- Author
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Wang J, Sun YR, Tao LG, Liu AW, and Hu SM
- Abstract
Precise molecular transition frequencies are needed in various studies including the test of fundamental physics. Two well isolated ro-vibrational transitions of
12 C16 O at 1.57 μm, R(9) and R(10) in the second overtone band, were measured by a comb-locked cavity ring-down spectrometer. Despite the weakness of the lines (Einstein coefficient A≃0.008 s-1 ), Lamb-dip spectra were recorded with a signal-to-noise ratio over 1000, and the line positions were determined to be 191 360 212 761.1 and 191 440 612 662.2 kHz, respectively, with an uncertainty of 0.5 kHz (δν/ν=2.6×10-12 ). The present work demonstrates the possibility to explore extensive molecular lines in the near-infrared with sub-kHz accuracy.- Published
- 2017
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85. [Potassium channels and autophagy].
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Yang S, Liu J, Qu C, Sun J, Zhang BQ, Sun YR, and Zou W
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- Animals, Homeostasis, Humans, Phagosomes, Autophagy, Potassium Channels physiology, Signal Transduction
- Abstract
Potassium channels have multitudinous subtypes, which are widely distributed on cell membranes. They affect various cellular physiological functions through participating in processes such as resting potential formation, substance transportation, enzyme activity and cellular communication. Autophagy is a significant mechanism to maintain intracellular metabolic homeostasis. The abnormity of autophagy may lead to the occurrence and development of multiple diseases. Recently, it has been reported that K
+ channels and cell autophagy are closely related. Here, we reviewed the recent research progresses on regulation of autophagy signaling, autophagy flux or autophagolysosome formation by K+ channel, and discussed the physiological significance of autophagy regulation by K+ channel.- Published
- 2017
86. [Effect of nicorandil on ventricular arrhythmia in patients with acute ST-segment elevation myocardial infarction underwent emergent percutaneous coronary intervention treatment].
- Author
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Wang YP, Zhang Y, Sun YR, Sun ZG, Zuo ZK, Feng ZR, Chang FY, Xu YC, Chen BZ, and Ye YY
- Subjects
- Arrhythmias, Cardiac, Humans, Myocardial Infarction, Treatment Outcome, Nicorandil therapeutic use, Percutaneous Coronary Intervention, ST Elevation Myocardial Infarction, Tachycardia, Ventricular drug therapy
- Abstract
Objective: To investigate the effect of nicorandil on ventricular arrhythmia in patients with acute ST-segment elevation myocardial infarction (STEMI) treated with emergent percutaneous coronary intervention (PCI). Methods: A total of 120 acute STEMI patients treated with emergent PCI in our hospital from January 2015 to June 2016 were randomly divided into control group and experiment group ( n =60 each). Patients in both groups received conventional therapy.Patients in experiment group took 10 mg nicorandil orally before PCI and received oral nicorandil treatment (15 mg/d, three times daily) for 3 days.QT disperse(QTd), correct QTd(QTcd) and the occurrence rate of ventricular arrhythmia were compared between two groups. Results: QTd at 6, 24, 48 and 72 hours((70.6±4.4), (67.2±5.3), (55.7±8.5), (48.2±8.2) ms, respectively) after PCI was significantly lower in the experiment group than those of control group ((77.1±7.1), (71.3±6.5), (65.1±8.1), (57.2±5.4) ms, all P <0.05). The level of QTd was also significantly lower in the experiment group at 6, 24, 48 and 72 hours((77.5±7.7), (67.7±8.6), (61.2±7.5), (52.9±8.4) ms, respectively) after PCI comared to those of control group ((88.6±8.1), (79.2±7.8), (74.4±7.4), (69.6±8.6) ms, all P <0.05). There was no significant difference in the incidence of reperfusion arrhythmia during PCI procedure between the two groups.The prevalence of the ventricular premature beat in the experiment group (25/60, 41.7%) was significantly lower than in the control group(45/60, 75.0%) within 3 days after PCI( P <0.01), the prevalence of the no sustained ventricular tachycardia and ventricular fibrillation in the experiment group(6/60, 10.0%) was also significantly lower than in the control group (18/60, 30.0%, P <0.01) within 3 days after PCI. Conclusions: Nicorandil use prior and post PCI could decrease the occurrence rate of ventricular arrhythmia in STEMI patients undergoing emergent PCI, and this effect might be related with reduced QTd and QTcd post medication.
- Published
- 2017
- Full Text
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87. Potent Nematicidal Activity and New Hybrid Metabolite Production by Disruption of a Cytochrome P450 Gene Involved in the Biosynthesis of Morphological Regulatory Arthrosporols in Nematode-Trapping Fungus Arthrobotrys oligospora.
- Author
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Song TY, Xu ZF, Chen YH, Ding QY, Sun YR, Miao Y, Zhang KQ, and Niu XM
- Subjects
- Animals, Antinematodal Agents chemistry, Ascomycota genetics, Ascomycota growth & development, Ascomycota metabolism, Biosynthetic Pathways, Cytochrome P-450 Enzyme System genetics, Mutation, Nematoda growth & development, Pest Control, Biological, Polyketide Synthases genetics, Polyketide Synthases metabolism, Spores, Fungal enzymology, Spores, Fungal genetics, Spores, Fungal growth & development, Spores, Fungal metabolism, Antinematodal Agents metabolism, Ascomycota enzymology, Cytochrome P-450 Enzyme System metabolism, Fungal Proteins metabolism, Nematoda microbiology
- Abstract
Types of polyketide synthase-terpenoid synthase (PKS-TPS) hybrid metabolites, including arthrosporols with significant morphological regulatory activity, have been elucidated from nematode-trapping fungus Arthrobotrys oligospora. A previous study suggested that the gene cluster AOL_s00215 in A. oligospora was involved in the production of arthrosporols. Here, we report that disruption of one cytochrome P450 monooxygenase gene AOL_s00215g280 in the cluster resulted in significant phenotypic difference and much aerial hyphae. A further bioassay indicated that the mutant showed a dramatic decrease in the conidial formation but developed numerous traps and killed 85% nematodes within 6 h in contact with prey, in sharp contrast to the wild-type strain with no obvious response. Chemical investigation revealed huge accumulation of three new PKS-TPS epoxycyclohexone derivatives with different oxygenated patterns around the epoxycyclohexone moiety and the absence of arthrosporols in the cultural broth of the mutant ΔAOL_s00215g280. These findings suggested that a study on the biosynthetic pathway for morphological regulatory metabolites in nematode-trapping fungus would provide an efficient way to develop new fungal biocontrol agents.
- Published
- 2017
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88. Comb-locked cavity ring-down saturation spectroscopy.
- Author
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Wang J, Sun YR, Tao LG, Liu AW, Hua TP, Meng F, and Hu SM
- Abstract
We present a new method of comb-locked cavity ring-down spectroscopy for the Lamb-dip measurement of molecular ro-vibrational transitions. By locking both the probe laser frequency and a temperature-stabilized high-finesse cavity to an optical frequency comb, we realize saturation spectroscopy of molecules with kilohertz accuracy. The technique is demonstrated by recording the R(9) line in the υ = 3 - 0 overtone band of CO near 1567 nm. The Lamb-dip spectrum of such a weak line (transition rate 0.0075 s
-1 ) is obtained using an input laser power of only 3 mW, and the position is determined to be 191 360 212 770 kHz with an uncertainty of 7 kHz (δν/ν∼3.5×10-11 ), which is currently limited by our rubidium clock.- Published
- 2017
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89. Laser Spectroscopy of the Fine-Structure Splitting in the 2^{3}P_{J} Levels of ^{4}He.
- Author
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Zheng X, Sun YR, Chen JJ, Jiang W, Pachucki K, and Hu SM
- Abstract
The fine-structure splitting in the 2^{3}P_{J} (J=0, 1, 2) levels of ^{4}He is of great interest for tests of quantum electrodynamics and for the determination of the fine-structure constant α. The 2^{3}P_{0}-2^{3}P_{2} and 2^{3}P_{1}-2^{3}P_{2} intervals are measured by laser spectroscopy of the ^{3}P_{J}-2^{3}S_{1} transitions at 1083 nm in an atomic beam, and are determined to be 31 908 130.98±0.13 kHz and 2 291 177.56±0.19 kHz, respectively. Compared with calculations, which include terms up to α^{5}Ry, the deviation for the α-sensitive interval 2^{3}P_{0}-2^{3}P_{2} is only 0.22 kHz. It opens the window for further improvement of theoretical predictions and an independent determination of the fine-structure constant α with a precision of 2×10^{-9}.
- Published
- 2017
- Full Text
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90. Measurements of Ion-Motive Force Across the Cell Membrane.
- Author
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Lin TS, Sun YR, and Lo CJ
- Subjects
- Bacteria metabolism, Flagella metabolism, Ions metabolism, Membrane Potentials physiology, Models, Biological, Molecular Motor Proteins metabolism, Protons, Sodium metabolism, Cell Membrane metabolism, Movement physiology
- Abstract
Cells need energy to survive. Ion-motive force (IMF) is one of the most important biological energy formats in bacterial cells. Essentially, the ion-motive force is the sum of electrical and chemical potential differences across the cell membrane. For bacteria, the ion-motive force is involved not only in ATP production but also in flagellar motility. The bacterial flagellar motor is driven either by proton or sodium ion. The ion-motive force measurement therefore requires the measurement of membrane potential, proton concentration, or sodium ion concentration. The bacterial flagellar motor is the most powerful molecular machine we have known so far. To understand the energetic condition of bacterial flagellar motors, together with single-motor torque measurement, methods for single-cell ion-motive force measurement have been developed. Here, we describe fluorescent approaches to measure the components of ion-motive force.
- Published
- 2017
- Full Text
- View/download PDF
91. A critical appraisal of the methodology and quality of evidence of systematic reviews and meta-analyses of traditional Chinese medical nursing interventions: a systematic review of reviews.
- Author
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Jin YH, Wang GH, Sun YR, Li Q, Zhao C, Li G, Si JH, Li Y, Lu C, and Shang HC
- Subjects
- Chronic Disease nursing, Evidence-Based Medicine, Humans, Practice Guidelines as Topic, Quality Control, Review Literature as Topic, Chronic Disease therapy, Medicine, Chinese Traditional
- Abstract
Objective: To assess the methodology and quality of evidence of systematic reviews and meta-analyses of traditional Chinese medical nursing (TCMN) interventions in Chinese journals. These interventions include acupressure, massage, Tai Chi, Qi Gong, electroacupuncture and use of Chinese herbal medicines-for example, in enemas, foot massage and compressing the umbilicus., Design: A systematic literature search for systematic reviews and meta-analyses of TCMN interventions was performed. Review characteristics were extracted. The methodological quality and the quality of the evidence were evaluated using the Assessment of Multiple Systematic Reviews (AMSTAR) and Grading of Recommendations Assessment, Development and Evaluation (GRADE) approaches., Result: We included 20 systematic reviews and meta-analyses, and a total of 11 TCMN interventions were assessed in the 20 reviews. The compliance with AMSTAR checklist items ranged from 4.5 to 8 and systematic reviews/meta-analyses were, on average, of medium methodological quality. The quality of the evidence we assessed ranged from very low to moderate; no high-quality evidence was found. The top two causes for downrating confidence in effect estimates among the 31 bodies of evidence assessed were the risk of bias and inconsistency., Conclusions: There is room for improvement in the methodological quality of systematic reviews/meta-analyses of TCMN interventions published in Chinese journals. Greater efforts should be devoted to ensuring a more comprehensive search strategy, clearer specification of the interventions of interest in the eligibility criteria and identification of meaningful outcomes for clinicians and patients (consumers). The overall quality of evidence among reviews remains suboptimal, which raise concerns about their roles in influencing clinical practice. Thus, the conclusions in reviews we assessed must be treated with caution and their roles in influencing clinical practice should be limited. A critical appraisal of systematic reviews/meta-analyses of TCMN interventions is particularly important to provide sound guidance for TCMN., Competing Interests: Conflicts of Interest: None declared., (Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.)
- Published
- 2016
- Full Text
- View/download PDF
92. [Effect of age on urodynamic parameters of women with urinary incontinence].
- Author
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Zhang WY, Zhang XP, Chen JW, Sun YR, Wang J, Hu H, and Xu KX
- Subjects
- Age Factors, Aged, Female, Hospitals, University, Humans, Middle Aged, Urinary Bladder physiopathology, Urination physiology, Urinary Incontinence diagnosis, Urinary Incontinence epidemiology, Urinary Incontinence physiopathology, Urodynamics
- Abstract
Objective: To investigate age related changes in urodynamic parameters of women with urinary incontinence., Methods: From May 2008 to October 2015, a total of 214 patients diagnosed with urinary incontinence in Peking University People's Hospital was involved in this study. Average age was (56.97±10.68) years, ranging from 30 to 82 years, and average history was (8.44±8.85) years, ranging from one month to 50 years. Urodynamic examinations of each patient were taken before operation routinely in Department of Urology, Peking University People's Hospital. The urodynamic study was composed of non-invasive and invasive procedures. Analysis included maximal flow rate (Qmax), average flow rate, time to Qmax, voiding time, detrusor pressure at Qmax, maximal detrusor pressure, voided volume, post-void residual urine volume (PVR), the total capacity of bladder, first-, strong-, and urge-desire to void, cough leak point pressure (CLPP), and Valsalva leak point pressure (VLPP). Patients were divided into four groups according to age, Kolmogorov-Smirnov test and one-way ANOVA were used for data analysis., Results: A total of 214 patients were enrolled in this study. The data of Qmax, average flow rate, voided volume, and total capacity of bladder decreased with statistical significance. The value of residual urine volume and voiding time increased without statistical significance, while the value of maximal detrusor pressure decreased., Conclusion: Urodynamic examination data of females with urinary incontinence changes along with the elapse of age, which was mainly observed as age ascends, and the changes in urodynamic parameters of women with urinary incontinence suggest that the value of Qmax, average flow rate, voided volume, and total capacity of bladder decreased significantly, while the value of PVR and the voiding time increased and the value of maximal detrusor pressure decreased.
- Published
- 2016
93. A novel curcumin analog binds to and activates TFEB in vitro and in vivo independent of MTOR inhibition.
- Author
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Song JX, Sun YR, Peluso I, Zeng Y, Yu X, Lu JH, Xu Z, Wang MZ, Liu LF, Huang YY, Chen LL, Durairajan SS, Zhang HJ, Zhou B, Zhang HQ, Lu A, Ballabio A, Medina DL, Guo Z, and Li M
- Subjects
- Animals, Autophagy, Brain metabolism, Cell Nucleus metabolism, HeLa Cells, Humans, Lysosomes metabolism, Male, Mice, Neurodegenerative Diseases metabolism, Phosphorylation, Protein Binding, Rats, Rats, Sprague-Dawley, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors metabolism, Curcumin chemistry, TOR Serine-Threonine Kinases metabolism
- Abstract
Autophagy dysfunction is a common feature in neurodegenerative disorders characterized by accumulation of toxic protein aggregates. Increasing evidence has demonstrated that activation of TFEB (transcription factor EB), a master regulator of autophagy and lysosomal biogenesis, can ameliorate neurotoxicity and rescue neurodegeneration in animal models. Currently known TFEB activators are mainly inhibitors of MTOR (mechanistic target of rapamycin [serine/threonine kinase]), which, as a master regulator of cell growth and metabolism, is involved in a wide range of biological functions. Thus, the identification of TFEB modulators acting without inhibiting the MTOR pathway would be preferred and probably less deleterious to cells. In this study, a synthesized curcumin derivative termed C1 is identified as a novel MTOR-independent activator of TFEB. Compound C1 specifically binds to TFEB at the N terminus and promotes TFEB nuclear translocation without inhibiting MTOR activity. By activating TFEB, C1 enhances autophagy and lysosome biogenesis in vitro and in vivo. Collectively, compound C1 is an orally effective activator of TFEB and is a potential therapeutic agent for the treatment of neurodegenerative diseases.
- Published
- 2016
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94. Coagulopathy in Traumatic Brain Injury and Its Correlation with Progressive Hemorrhagic Injury: A Systematic Review and Meta-Analysis.
- Author
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Yuan Q, Sun YR, Wu X, Yu J, Li ZQ, Du ZY, Wu XH, Zhou LF, and Hu J
- Subjects
- Humans, Blood Coagulation Disorders blood, Blood Coagulation Disorders epidemiology, Blood Coagulation Disorders etiology, Brain Injuries, Traumatic blood, Brain Injuries, Traumatic complications, Brain Injuries, Traumatic epidemiology, Intracranial Hemorrhage, Traumatic blood, Intracranial Hemorrhage, Traumatic complications, Intracranial Hemorrhage, Traumatic epidemiology
- Abstract
The association between coagulopathy and either isolated traumatic brain injury (TBI) or progressive hemorrhagic injury (PHI) remains controversial. The aims of this study were to evaluate whether isolated TBI induces pronounced coagulopathy, in comparison with non-TBI or TBI in conjunction with other injuries (TBI + other injuries), and to examine whether there is any evidence of a relationship between coagulopathy and PHI in patients who have experienced TBI. The MEDLINE(®) and Embase databases, and the Cochrane Central Register of Controlled Trials (Central), were trawled for relevant studies. Searches covered the period from the inception of each of the databases to June 2015, and were conducted using appropriate combinations of terms and key words based on medical subject headings (MeSH). Studies were included if they compared isolated TBI with a similar severity of injury to other body regions, or compared PHI with non-PHI, with regard to coagulation tests and the prevalence of coagulopathy. We extracted the means and standard deviations (SD) of coagulation test levels, as well as their ranges or the percentage of abnormal coagulation tests, in both cases and controls. A total of 19 studies were included in our systematic review and meta-analysis. Only the mean fibrinogen (FIB) in isolated TBI was found to be significantly higher than in TBI + other injuries (pooled mean difference [MD] 32.09; 95% confidence interval [CI] 4.92-59.25; p = 0.02); in contrast, it was also significantly higher than in non-TBI (pooled MD 15.44; 95% CI 0.28-30.59; p = 0.05). We identified 15 studies that compared coagulopathy between a PHI group and a non-PHI group. The PHI group had a lower platelet count (PLT) value (pooled MD -19.21; 95% CI: -26.99 to -11.44, p < 0.001) and a higher international normalized ratio (INR) value (pooled MD 0.07; 95% CI: 0.02-0.13, p = 0.006) than the non-PHI group, but no differences were observed in the mean activated partial thromboplastin time (APTT) and prothrombin time (PT) between the PHI and non-PHI patients. In addition, PHI was significantly associated with a higher percentage of INR >1.2 (pooled OR 3.49 [95% CI 1.97-6.20], p < 0.001), PLT <100 × 109/L (pooled OR 4.74 [95% CI 2.44-9.20], p < 0.001), and coagulopathy (pooled OR 2.52; 95% CI 1.88- 3.38; p < 0.001), compared with non-PHI. The current clinical evidence does not indicate that the prevalence of coagulopathy in TBI is significantly higher than in injuries of similar severity to other areas of the body, or in multiple injuries with TBI. With respect to the association between coagulopathy and PHI, the occurrence of coagulopathy, INR, and PLT was significantly associated with PHI, but APTT and PT were not found to be associated with PHI. In the future, high quality research will be required to further characterize the effects of coagulopathy on TBI and subsequent PHI.
- Published
- 2016
- Full Text
- View/download PDF
95. [Learning and Memory Capacity and NMDA Receptor Expression in Shen Deficiency Constitution Rats].
- Author
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Sun YR, Sun YG, Zhang Q, Wang XD, Wang X, and Sun LJ
- Subjects
- Animals, Female, Pregnancy, Random Allocation, Rats, Rats, Sprague-Dawley, Up-Regulation, Drugs, Chinese Herbal pharmacology, Learning drug effects, Memory drug effects, Receptors, N-Methyl-D-Aspartate metabolism
- Abstract
Objective: To explore material bases and neurobiological mechanisms of "Shen storing will" by observing learning and memory capacities and N-methyl-D-aspartic acid (NMDA) receptor expressions in Shen deficiency constitution (SDC) rats., Methods: Totally 40 SD rats were randomly divided into the model group, the Zuogui Pill (ZP) group, the Yougui Pill (YP) group, the blank control group (consisting of normal pregnant rats), 10 in each group. SDC young rat model (inherent deficiency and postnatal malnutrition) was prepared by the classic way of "cat scaring rat". Medication started when they were scared by cat. Rats in the ZP group and the YP group were administered by gastrogavage with ZP suspension 0.1875 g/mL and YP suspension 0.0938 g/mL respectively. Equal volume of normal saline was administered to rats in the blank control group and the model group by gastrogavage. All medication was given once per day, 5 days in a week for 2 consecutive months. Learning and memory capacities were detected by Morris water maze test. Expressions of NMDA receptor subunits NR2A and NR2B in hippocamus were detected by immunohistochemical method., Results: Compared with the blank control group, the latency period, total distance in Morris water maze test were longer in the model group (P < 0.05). All the aforesaid indices all decreased in the ZP group and the YP group, with statistical difference when compared with the model group (P < 0.05). The protein expressions of NR2A and NR2B in hippocamus were lower in the model group than in the blank control group (P < 0.05). But when compared with the model group, they were obviously higher in the ZP group and the YP group (P < 0.05)., Conclusions: SDC rats had degenerated learning and memory capacities and lowered NMDA receptor expressions. ZP and YP could up-regulate learning and memory capacities and NMDA receptor expressions, thereby improving deterioration of brain functions in SDC rats.
- Published
- 2016
96. Different Expression of MicroRNA-146a in Peripheral Blood CD4(+) T Cells and Lesions of Oral Lichen Planus.
- Author
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Yang JG, Sun YR, Chen GY, Liang XY, Zhang J, and Zhou G
- Subjects
- Adult, Female, Genetic Markers genetics, Humans, Lichen Planus, Oral pathology, Male, MicroRNAs biosynthesis, Middle Aged, Real-Time Polymerase Chain Reaction, Young Adult, CD4-Positive T-Lymphocytes immunology, Lichen Planus, Oral immunology, MicroRNAs genetics
- Abstract
Oral lichen planus (OLP) is a common T cell-mediated chronic inflammatory disease of unknown etiology. Recent increasing evidence indicates that microRNA-146a (miR-146a) plays a vital role in inflammatory diseases and T cell regulation. This study aimed to investigate the expression of miRNA-146a in peripheral blood CD4(+) T cells and local OLP lesions and to evaluate its relationship with clinical forms of OLP. Sixteen patients with OLP were divided into two groups: erosive OLP and nonerosive OLP. The expression of miR-146a was examined by quantitative real-time polymerase chain reaction. The expression of miR-146a in peripheral blood CD4(+) T cells showed no significant difference between OLP group and control group (P > 0.05), and among erosive OLP, nonerosive OLP, and control groups (P > 0.05 for all). The expression in local lesions of the OLP group was significantly higher than that of the control group (P = 0.003), and it was significantly higher in the erosive OLP group than in the non-erosive OLP (P = 0.010) and control groups (P = 0.007). However, miR-146a expression in the nonerosive OLP group did not significantly differ from that in the control group (P > 0.05). These data indicate that miR-146a might be more involved in the local immune disorder of OLP. MiR-146a might be utilized as a candidate biomarker to estimate the severity of OLP.
- Published
- 2016
- Full Text
- View/download PDF
97. In vivo and in vitro effect of hepatocarcinoma lymph node metastasis by upregulation of Annexin A7 and relevant mechanisms.
- Author
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Wang XY, Gao F, Sun YR, Bai LL, Ibrahim MM, Wang B, and Tang JW
- Subjects
- Animals, Cell Line, Tumor, Cell Movement, Cell Proliferation, Enzyme-Linked Immunosorbent Assay, Female, Immunohistochemistry, Lymphatic Metastasis, Male, Mice, Neoplasm Invasiveness, Neoplasm Metastasis, Prognosis, Up-Regulation, Vascular Endothelial Growth Factor C metabolism, Annexin A7 metabolism, Carcinoma, Hepatocellular metabolism, Carcinoma, Hepatocellular pathology, Liver Neoplasms metabolism, Liver Neoplasms pathology
- Abstract
We unveiled the association of Annexin A7 with vascular endothelial growth factor-C (VEGF-C) and the effect of upregulation of Annexin A7 in Hca-F and Hca-P cells on inhibiting hepatocarcinoma (HCC) lymph node metastasis (LNM) in vitro and in vivo. A total of 200 inbred 615 mice were randomly divided into four equal groups inoculated with Hca-F, Hca-P, FAnxa7-upregulated, and PAnxa7-upregulated cells, respectively. The primary tumor, popliteal, inguinal, and iliac lymph nodes were prepared for immunohistochemical (IHC) staining, real-time quantitative polymerase chain reaction (qRT-PCR) analysis, Western blot, and hematoxylin-eosin (H&E) staining. There was over 50 % increase both in the number of FAnxa7-upregulated and PAnxa7-upregulated cells migrated through the filter compared to their controls (FAnxa7-control, Hca-F and PAnxa7-control, Hca-P). However, no significant differences were noted in invasion ability between them (all P > 0.05). Tumor lymph vessels were significantly reduced in FAnxa7-upregulated and PAnxa7-upregulated tumors when compared with Hca-F and Hca-P tumors (all P < 0.05). Blood vessel density did not differ significantly between FAnxa7-upregulated and PAnxa7-upregulated tumors and Hca-F and Hca-P tumors. Enzyme-linked immunosorbent assay (ELISA) for VEGF-C showed that upregulating Annexin A7 decreased VEGF-C secretion in FAnxa7-upregulated and PAnxa7-upregulated cells (P < 0.05). The IHC staining result showed that the level of serum Annexin A7 was found to be statistically higher in all experimental groups than that in the control group (P < 0.05). The present results indicated that alterations in serum Annexin A7 expression may be of prognostic relevance in HCC lymphatic metastasis.
- Published
- 2016
- Full Text
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98. Alternating chiral selectivity of aldol reactions under the confined space of mesoporous silica.
- Author
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Yeh C, Sun YR, Huang SJ, Tsai YM, and Cheng S
- Abstract
The chiral selectivities were altered and high diastereo- and enantio-selectivities of the products were obtained in water medium without adding acid co-catalysts when a primary-tertiary diamine catalyst was immobilized on mesoporous SBA-15 to form a recyclable catalyst for the direct asymmetric aldol reaction of cyclohexanone with p-nitrobenzaldehyde.
- Published
- 2015
- Full Text
- View/download PDF
99. Ad-PUMA sensitizes ovarian cancer cells to chemotherapeutic agents.
- Author
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Luan QC, Sun YR, Han P, and Chen Y
- Subjects
- Adenoviridae, Animals, Antineoplastic Agents therapeutic use, Apoptosis drug effects, Apoptosis genetics, Apoptosis Regulatory Proteins metabolism, Apoptosis Regulatory Proteins therapeutic use, Cell Line, Tumor, Cisplatin pharmacology, Cisplatin therapeutic use, Combined Modality Therapy, Female, Genetic Therapy, Humans, Ovarian Neoplasms genetics, Ovarian Neoplasms therapy, Paclitaxel pharmacology, Paclitaxel therapeutic use, Proto-Oncogene Proteins metabolism, Proto-Oncogene Proteins therapeutic use, Transgenes genetics, Up-Regulation drug effects, Up-Regulation genetics, Antineoplastic Agents pharmacology, Apoptosis Regulatory Proteins genetics, Ovarian Neoplasms pathology, Proto-Oncogene Proteins genetics
- Abstract
Objective: Ovarian cancer accounted for the first cause of death in female reproductive system tumor even with the operation and chemotherapy. We sought to evaluate the therapeutic potential of p53 up-regulated modulator of apoptosis (PUMA) in ovarian cancer., Materials and Methods: An adenovirus expressing PUMA (Ad-PUMA), alone or in combination with chemotherapeutic agents, was used to treat two different ovarian cancer cell lines. The mechanism of PUMA-mediated growth suppression and apoptosis was investigated by analysis of caspase-9 activation and the change of mitochondrial membrane potential (Δψm)., Results: The exogenous PUMA was expressed 6 h after Ad-PUMA infection, which increased the chemosensitivity of the cancer cells and decreased the IC50 of chemotherapeutic agents compared with uninfected cells. The apoptotic percentage of OVCAR-3 and SKOV3 increased greatly compared with Taxol or Cisplatin alone. There was shear zone in caspase-9 and Δψm decrease after Ad-PUMA infection which suggested apoptosis started in mitochondrial mediated pathway., Conclusions: PUMA plays a role in suppressing tumor growth and sensitizing ovarian cancer cells to anticancer drugs and may be a promising tool for cancer biotherapy.
- Published
- 2015
100. Ralstonia solanacearum fatty acid composition is determined by interaction of two 3-ketoacyl-acyl carrier protein reductases encoded on separate replicons.
- Author
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Feng SX, Ma JC, Yang J, Hu Z, Zhu L, Bi HK, Sun YR, and Wang HH
- Subjects
- Escherichia coli genetics, Escherichia coli metabolism, Gene Deletion, Genetic Complementation Test, Solanum lycopersicum microbiology, Plant Diseases microbiology, Ralstonia solanacearum genetics, Recombinant Proteins genetics, Recombinant Proteins isolation & purification, Recombinant Proteins metabolism, Virulence, 3-Oxoacyl-(Acyl-Carrier-Protein) Reductase genetics, 3-Oxoacyl-(Acyl-Carrier-Protein) Reductase metabolism, Fatty Acids analysis, Ralstonia solanacearum chemistry, Ralstonia solanacearum enzymology, Replicon
- Abstract
Background: FabG is the only known enzyme that catalyzes reduction of the 3-ketoacyl-ACP intermediates of bacterial fatty acid synthetic pathways. However, there are two Ralstonia solanacearum genes, RSc1052 (fabG1) and RSp0359 (fabG2), annotated as encoding putative 3-ketoacyl-ACP reductases. Both FabG homologues possess the conserved catalytic triad and the N-terminal cofactor binding sequence of the short chain dehydrogenase/reductase (SDR) family. Thus, it seems reasonable to hypothesize that RsfabG1 and RsfabG2 both encode functional 3-ketoacyl-ACP reductases and play important roles in R. solanacearum fatty acid synthesis and growth., Methods: Complementation of Escherichia coli fabG temperature-sensitive mutant with R. solanacearum fabGs encoded plasmids was carried out to test the function of RsfabGs in fatty acid biosynthesis. RsFabGs proteins were purified by nickel chelate chromatography and fatty acid biosynthetic reaction was reconstituted to investigate the 3-ketoacyl-ACP reductase activity of RsFabGs in vitro. Disruption of both RsfabG genes was done via DNA homologous recombination to test the function of both RsfabG in vivo. And more we also carried out pathogenicity tests on tomato plants using RsfabG mutant strains. , Results: We report that expression of either of the two proteins (RsFabG1 and RsFabG2) restores growth of the E. coli fabG temperature-sensitive mutant CL104 under non-permissive conditions. In vitro assays demonstrate that both proteins restore fatty acid synthetic ability to extracts of the E. coli strain. The RsfabG1 gene carried on the R. solanacearum chromosome is essential for growth of the bacterium, as is the case for fabG in E. coli. In contrast, the null mutant strain with the megaplasmid-encoded RsfabG2 gene is viable but has a fatty acid composition that differs significantly from that of the wild type strain. Our study also shows that RsFabG2 plays a role in adaptation to high salt concentration and low pH, and in pathogenesis of disease in tomato plants., Conclusion: R. solanacearum encodes two 3-ketoacyl-ACP reductases that both have functions in fatty acid synthesis. We supply the first evidence that, like other enzymes in the bacterial fatty acid biosynthetic pathway, one bacterium may simultaneously possess two or more 3-oxoacyl-ACP reductase isozymes.
- Published
- 2015
- Full Text
- View/download PDF
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