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To restore species-rich terrestrial ecosystems on ex-agricultural land, establishing nutrient limitation for dominant plant growth is essential because in nutrient-rich soils, fast-growing species often exclude target species. However, N-limitation is easier to achieve than P-limitation (because of a difference in biogeochemical behavior), biodiversity is generally highest under P-limitation. Commonly used restoration methods to achieve low soil P-concentrations are either very expensive or take a very long time. A promising restoration technique is P-mining, an adjusted agricultural technique that aims at depleting soil-P. High biomass production and hence high P-removal with biomass are obtained by fertilizing with nutrients other than P. A pot experiment was set up to study P-mining with Lolium perenne L. on sandy soils with varying P-concentrations: from an intensively used agricultural soil to a soil near the soil P-target for species-rich Nardus grassland. All pots received N- and K-fertilization. The effects of biostimulants on P-uptake were also assessed by the addition of arbuscular mycorrhiza (Glomus spp.), humic substances or phosphate-solubilizing bacteria (Bacillus sp. and Pseudomonas spp.). In our P-rich soil (111 µg POlsen/g), P-removal rate was high but bioavailable soil-P did not decrease. At lower soil P-concentrations (64 and 36 µg POlsen/g), bioavailable soil-P had decreased but the P-removal rate had by then dropped 60% despite N- and K-fertilization and despite that the target (

Gamma irradiation is becoming a potential tool in soil ecological studies as it gives a possibility to vary a dose that selectively kills the target organism. It makes minimum changes in soil biochemical properties as compared to autoclaving and freezing. Although gamma irradiation has often been used in studying the roles of nematodes on nutrient cycling, the recommended doses to eliminate nematodes still lead to a nutrient flush, and are less reproducible. Given that the indirect effect of gamma irradiation is through radiolysis, the same dose might have a different effect on the soil biochemical properties as the soil moisture content changes. Thus, an optimal dose that eliminates nematodes needs to be determined taking the moisture content of the soil sample at the time of irradiation into consideration. We conducted an incubation experiment for about three months during which the effects of a range of low gamma irradiation doses (0, 1, 3 and 5 kGy) at different moisture content (air dried, 50% water filled pore space (WFPS), 80%WFPS) were tested on nematode abundance and selected soil biochemical properties. Leaching with water immediately after irradiation at 50%WFPS, was used to assess to what extent it would remove N from the flush. The results showed that as the moisture content of the soil increases from 50%WFPS to 80%WFPS, nematode abundance, enzyme activities and total mineral N concentrations decreased during most of the incubation period at each irradiation dose. Increasing the moisture content, however, did not make significant changes in total phospholipid fatty acid concentrations in all levels of irradiations except at 3 kGy. These findings indicate the effects of gamma irradiation on soil biological and chemical properties vary depending on the moisture content of the soil at the time irradiation. Moreover, the moisture content of the soil at the time of gamma irradiation need to be considered during optimizing gamma irradiation as a tool in selective sterilization or defaunation. In the current experimental set up, the application of 3 kGy at 80%WFPS at the time of irradiation eradicated nematodes and left a comparable microbial abundance and community structure to the unirradiated CTR. Leaching the 50%WFPS samples irradiated at 5 kGy dose immediately after irradiation generally significantly reduced both NH 4 + -N and NO 3 − -N concentrations, but NH + 4 -N concentration remained higher compared to the unirradiated CTR. This indicates that leaching with water only partly reduced the nutrient flush and underlines the need to test additional methods to reduce the nutrient flush further.

To evaluate the effect of farming system on gaseous emission of N from soil, the measurements of nitrous oxide (N2O) were taken in organic and conventional paddy fields in tropics. Eleven soil samples from organic and neighboring conventional sites in Central Java, Indonesia were incubated at two different moisture contents expressed as water filled pores (WFPS), namely 75% WFPS and 100% WFPS. Emission of N2O-N at 75% WFPS was significantly higher in conventional management than in organic management. At 100% WFPS, the N2O-N emission was also higher in conventional than in organic farming system. Although it was not significantly different, it has significantly and positively correlations with SOC, total N and initial mineral N content. Due to the swelling ability of the high clay which is contained in soil samples, less N2O-N emission in 75% WFPS than in 100% WFPS was observed in the present study. The range of N2O-N emission at 75% WFPS was 0.01 – 0.03 kg N2O-N/ha in organic farming and 0.005 – 0.16 kg N2O-N/ha in conventional farming; for organic farming at 100% WFPS, the N2O-N emission was 0.04 – 61 kg N2O-N/ha and in conventional farming was 0.08 – 74 kg N2O-N/ha. This study concludes that organic farming in paddy fields might have more promising potential to mitigate N2O-N emission than conventional farming in paddy fields.

Vegetable crop residues take a particular position relative to arable crops due to often large amounts of biomass with a N content up to 200 kg N ha−1 left behind on the field. An important amount of vegetable crops are harvested during late autumn and despite decreasing soil temperatures during autumn, high rates of N mineralization and nitrification still occur. Vegetable crop residues may lead to considerable N losses through leaching during winter and pose a threat to meeting water quality objectives. However, at the same time vegetable crop residues are a vital link in closing the nutrient and organic matter cycle of soils. Appropriate and sustainable management is needed to harness the full potential of vegetable crop residues. Two fundamentally different crop residue management strategies to reduce N losses during winter in intensive vegetable rotations are reviewed, namely (i) on-field management options and modifications to crop rotations and (ii) removal of crop residues, followed by a useful and profitable application.

The present study examines the effects of different fertilization treatments (chemical fertilization, farmyard manure, plant compost, and mycorrhiza-inoculated compost) on the soil fungi under a crop rotation of wheat (Triticum aestivum L.) and corn (Zea mays L.) in a long-term field experiment established in Mediterranean Turkey in 1996. Soil samples were collected in May, August, and October 2009. Soil pH, organic carbon, plant-available nitrogen and phosphorus, mycorrhizal colonization, and a series of biochemical markers (phospholipid and neutral lipid fatty acid [PLFA and NLFA] profiles, soil ergosterol content, and glomalin related soil protein [GRSP] as indicators of abundance of bacteria, saprotrophic, and arbuscular mycorrhizal [AM] fungi) were assessed. No significant difference was observed in soil organic C and plant available N in relation to long-term fertilization treatments, but plant available P in soil changed significantly in relation to the fertilization treatment used and the sampling season (between 11.5–33.8 mg · kg-1in spring, 10.4–28.6 mg · kg-1in summer, and 10.5–33.2 mg · kg-1in autumn). Mycorrhizal colonization patterns were similar for both plants. However, mycorrhiza-inoculated compost treatment exhibited higher root colonization (77.3%) over control (16.3%), chemical fertilization (10.0%), farmyard manure (19.3%), and plant compost (20.0%). No statistically significant change was observed in ergosterol content. The effect of long-term organic treatments on soil PLFA structure was statistically prominent; whereas seasonality only affected bacterial PLFAs. Organic fertilization increased GRSP (mean annual ranging from 0.91 to 2.46 mg · g-1total GRSP) but long-term annual mycorrhizal inoculation had no significant effect on the soil GRSP pool. © 2015, Taylor & Francis Group, LLC.

Historic long-term P application to sandy soils in NW-Europe has resulted in abundant sorption, saturation and eventually leaching of P from soil to the groundwater. Although many studies recognize the control of site-specific factors like soil texture and phosphate saturation degree (PSD), the regional-scaled relevance of effects exerted by single factors controlling P leaching is unclear. Very large observational datasets of soil and groundwater P content are furthermore required to reveal indirect controls of soil traits through mediating soil variables. We explored co-variation of phreatic groundwater orthophosphate (o-P) concentration and soil factors in sandy soils in Flanders, Belgium. Correlation analyses were complemented with an exploratory model derived using 'path analysis'. Data of oxalate-extractable Al, Fe, P and pHKCl, phosphate sorption capacity (PSC) and PSD in three depth layers (0-30, 30-60, 60-90 cm), topsoil SOC, % clay and groundwater depth (fluctuation) were interpolated to predict soil properties on exact locations of a very extensive net of groundwater monitoring wells. The mean PSD was only poorly correlated to groundwater o-P concentration, indicating the overriding control of other factors in the transport of P to the groundwater. A significant (P

The nitrogen status of most Zambian soils is inherently low. Nitrogen-fixing trees such as Faidherbia albida (F. albida) could have the potential to restore soil fertility. We conducted a study to examine the role of mature F. albida trees on the soil microbial communities and overall N fertility status in Zambia. Soil samples were collected under and outside the canopies of F. albida trees in representative fields from two sites namely; Chongwe (loamy sand) and Monze (sandy loam). To assess the long term canopy effects; total N, mineral N and soil organic carbon (Corg) content were directly measured from soils collected under and outside the canopy. Short term litter effects were assessed by subtracting concentrations of biochemical properties of non-amended controls from amended soils with F. albida litter during an 8 week incubation experiment. We also determined N mineralization rates, microbial community structure—Phospholipid fatty acids, microbial biomass carbon, and labile organic carbon ( $${\text{C}}_{{{\text{org[K}}_{ 2} {\text{SO}}_{ 4} ]}}$$ ) during incubation. For the long term canopy effect, average N mineralization rate, Corg, total N and mineral N content of non-amended soils under the canopy were (all significant at p

Soil quality in vegetable cropping systems is seriously threatened by intensive tillage and fertilization practices and by limited crop rotations. Inclusion of cover crops, compost application and reduced tillage may help to sustain soil quality. A three-year field trial was set up on horticultural land to explore the combined effects of compost amendment at three rates (0, 15 and 45 Mg ha−1 year−1) and tillage practices (reduced tillage versus conventional ploughing) on soil quality. Cover crop was not a factor in the experiment, but cover crops were included in the rotation for reasons of good agricultural practice. The highest compost dose supported the initial level of total organic carbon in the arable layer. The decrease in pH in the arable layer was considerably limited by compost application, irrespective of the dose applied. Reduced tillage resulted in a favorable stratification for different soil quality indicators both by placement of organic inputs near the soil surface and by a reduction of leaching of base cations and organic carbon compounds. Differences between tillage practices and compost doses were most striking in the 0–10 cm soil layer. Compost application at the highest rate enhanced organic C content by 16% compared to the content in the non-amended soil. Reduced tillage induced a 13% higher organic C content in the 0–10 cm soil layer than that in the underlying 10–30 cm layer. Combining reduced tillage and recurrent compost application resulted in a different soil microbial community structure in the 0–10 cm surface layer, as revealed by phospholipid fatty acids analysis. Total microbial biomass was 44% higher under reduced compared to conventional tillage and increased by 27% due to compost application at a rate of 45 Mg ha−1 year−1. Fungal biomass doubled in the surface layer by reduced tillage. Actinomycetes and arbuscular mycorrhizal fungi were favored by both reduced tillage and compost application. Conversion to reduced tillage allowed for sustaining crop production in this intensive vegetable cropping system. Compost application and reduced tillage counteracted soil degradation.

Judicious management of organic matter supply and tillage practice is crucial for sustaining soil quality in horticulture. We have studied whether N fertilization of vegetable crops should be adapted in the short term when soil management changes to include compost application and reduced tillage as soil quality improving factors. The experimental setup was a multiyear field trial on a sandy loam soil with a vegetable crop rotation. Soil tillage in spring was either conventional (moldboard plough) or reduced, non-inversion tillage (with a specially designed chisel plough). Starting in 2008, farm compost was applied each autumn at three rates: 0, 15 and 45 Mg per hectare. The three-year crop rotation (2009–2011) consisted of broccoli ( Brassica oleracea , var. Italica Group), carrots ( Daucus carota ) and leek ( Allium porrum ). Small but significant differences in N dynamics were found between treatments. Broccoli had a higher N uptake and biomass production under reduced compared to conventional tillage. In accordance with this, the apparent net N mineralization tended to be higher for reduced tillage compared to conventional tillage. In contrast, for leek, N uptake did not differ between tillage practices despite a higher apparent net N mineralization under conventional tillage. A tendency for a higher biomass production under conventional tillage was in line with a tendency for a higher amount of residual soil mineral N that might have increased the risk of N losses. In the carrot crop, a higher amount of mineral N under conventional tillage did not increase root yield. Compost application maintained soil organic matter content and did not result in a higher amount of residual soil mineral N. Only in the carrot growing season, compost application increased soil mineral N content to a limited extent. The small short-term changes in overall N availability observed between treatments do not necessitate changes in N fertilization strategy or additional precautions regarding residual soil mineral N.

This study, aiming to unravel whether topsoil conditions under tree species with nutrient-poor leaf litter can be altered by admixing a shrub layer, was performed in 12 pine (mainly Pinus sylvestris) and 12 oak (Quercus robur) stands on sandy podzolic soils in north-east Belgium. We examined the effects of presence of a shrub layer on forest floor mass and topsoil chemical properties related to soil acidification. The shrub species included were European rowan (Sorbus aucuparia), alder buckthorn (Rhamnus frangula) and black cherry (Prunus serotina). For each tree species, 60–90 years old stands were selected containing shrubs present in varying cover classes: sparse ( 70%). The oak stands were characterized by less but ‘nutrient-richer’ litterfall, compared to the pine stands. This was reflected in less humus build-up (FH-horizon) and higher pH, CEC, BS and lower C/N in the topsoil in the oak stands compared to the pine stands. However, despite the fact that the shrubs produced litter with significantly higher base cation and N concentrations than that of the studied tree species, we did not find any significant changes in topsoil conditions in the pine and oak stands under study, even under dense shrub layers (87–91% cover).

Rangeland degradation is a serious problem throughout sub-Saharan Africa and its restoration is a challenge for the management of arid and semi-arid areas. In Lake Baringo Basin of Kenya, communities and individual farmers are restoring indigenous vegetation inside enclosures in an effort to combat severe land degradation and address their livelihood problems. This study evaluated the impact of enclosure management on soil properties and microbial biomass, being key indicators of soil ecosystem health. Six reseeded communal enclosures using soil embankments as water-harvesting structures and strictly regulated access were selected, varying in age from 13 to 23 years. In six private enclosures, ranging from 3 to 17 years in age, individual farmers emulated the communal enclosure strategy and restored areas for their exclusive use. Significant decreases in bulk density, and increases in the soil organic carbon, total nitrogen and microbial biomass contents and stocks were found in the enclosures as compared with the degraded open rangeland. In the private enclosures, the impact of rehabilitation on the soil quality was variable, and soil quality was in general lower than that obtained under communal management. The significant increase of absolute stocks of carbon, nitrogen and microbial biomass compared to the degraded open rangeland indicates the potential for the restoration of soil quality through range rehabilitation. Over-sowing with indigenous legume fodder species could improve total nitrogen content in the soil and nutritional value of the pastures as well.

Several ecosystem processes such as nitrogen mineralization are mainly controlled by complex multitrophic interactions in which nematodes play major roles. Despite the abundance and diversity of these nematodes, studies on their contribution to N mineralization have been limited to a few selected species and often in completely sterilized media. Such simplified experiments are unrealistic and usually give inaccurate results as part of the interaction is missed. Therefore the contributions of nematodes to N mineralization need to be quantified more accurately in realistic conditions where native microbes, nematodes and plants interact. To do this, we set up a microcosm incubation experiment in which the whole nematode communities were extracted from fresh soil and reinoculated into the soil defaunated by 5 kGy dose gamma irradiation that leaves the microbial community largely intact. Three treatments namely control, +Nem (with nematodes) and −Nem (without nematodes), with or without plants were incubated for 86 days. In bare microcosms, +Nem cores increased total mineral nitrogen with 32% compared to −Nem. However, no significant ( p > 0.05) differences were observed on total mineral nitrogen and plant N uptake between +Nem and −Nem treatments in planted microcosms. Nematode abundances and community structures were significantly changed over time and differed in bare and planted microcosms. Bacterivores dominated in bare, while herbivores dominated in planted soils throughout the incubation period. This could explain the contrasting effects of nematodes in N mineralization in bare and planted microcosms. Optimization of gamma irradiation technique is required to further reduce its side effects on nutrient flush and non-targeted organisms.

The majority of microbial mediated soil processes depend on availability of organic matter (OM), water and air. Because of its ability to visualize the 3D architecture of soil non-destructively, X-ray computed tomography (CT) is becoming a widespread tool for studying soil pore network structure. However, phase determination of pore space, soil OM, soil mineral matter (MM) and water is often limited even with the latest technological and software advances, allowing high resolution and better quality imaging. Contrast agents commonly used in histology enable enhancement of X-ray attenuation of targeted structures or compounds. Here we report on the first systematic investigation of the use of such X-ray contrast agents for soil research. An evaluation procedure as well as a method to apply the agents to soil samples was developed and applied on reference soil samples. The effectiveness and selectivity of the contrast agents was evaluated for soil organic matter (SOM), MM and water. Several products were found to selectively increase the attenuation of water or SOM. The four agents with the best OM-staining capabilities (phosphomolybdenic acid (PMA), silver nitrate, lead nitrate and lead acetate) were further tested on an OM-MM mixture and all showed an increased of the SOM attenuation coefficient above the MM values.

Four biochar types, produced by slow pyrolysis of poultry litter (PL) and pine chips (P) at 400 or 500 °C, were added to two adjacent soils with contrasting soil organic matter (SOM) content (8.9 vs. 16.1 g C kg−1). The N mineralization rate was determined during 14-week incubations and assessments were made of the microbial biomass C, dehydrogenase activity, and the microbial community structure (PLFA-extraction). The addition of PL biochars increased the net N mineralization (i.e., compared to the control treatment) in both soils, while for treatments with P biochars net N immobilization was observed in both soils. Increasing the pyrolysis temperature of both feedstock types led to a decrease in net N mineralization. The ratio of Bacterial to Fungal PLFA biomarkers also increased with addition of biochars, and particularly in the case of the 500 °C biochars. Next to feedstock type and pyrolysis temperature, SOM content clearly affected the assessed soil biological parameters, viz. net N mineralization or immobilization, MBC and dehydrogenase activity were all greater in the H soil. This might be explained by an increased chance of physical contact between the microbial community activated by SOM mineralization upon incubation and discrete biochar particles. However, when considering the H soil's double C and N content, these responses were disproportionally small, which may be partly due to the L soil's, somewhat more labile SOM. Nonetheless, increasing SOM content and microbial biomass and activity generally appears to result in greater mineralization of biochar. Additionally, higher N mineralization after PL addition to the H soil with lower pH than the L soil can be due to the liming effect of the PL biochars.

The application rate of poultry manure as organic fertilizer is restricted due to its low C/P and N/P ratio. This research was set up to find out optimal feedstock composition for composting chicken manure in order to create a soil improver with a higher value as organic fertilizer, and to assess the effects of this process on P availability in the end products. The research is based on two compost experiments with three treatments each, and the chicken manure composts were compared with the end products of green waste compost used as bedding material in chicken houses, centrally processed chicken manure and a stockpiled mixture of green waste compost and chicken manure. In the first compost trial, feedstock materials were compared for composting with fresh chicken manure. The chicken manure compost with 42.5 vol% bark in the feedstock had the highest quality as soil improver in terms of organic matter content, C/P ratio, C/N ratio and stability. Results of the second compost trial indicate that 10 vol% of fresh chicken manure is the upper limit in small-scale on-farm windrow composting above which nutrient losses become too high and the N/P ratio of the obtained fertilizer becomes too low. Stability of the product (expressed as oxygen uptake rate) had a major effect on P availability in the compost. Blending the chicken manure compost with biochar based on holm oak resulted in a more than proportional decrease in easily available P in the biochar-blended compost. P in poultry manure can be recycled through composting and be applied as an organic fertilizer with optimal nutrient ratio and organic matter content when appropriate feedstock materials are selected and the amount of fresh manure in the feedstock mixture is restricted.

Soil fauna, particularly nematodes, are considered to strongly contribute to nitrogen mineralisation through grazing on microflora during decomposition. Demonstration of this effect has mostly relied on calculation-based soil food web analyses or experiments involving simplified and artificially constructed food webs. We carried out an incubation experiment in which defaunated soil cores were reinoculated with entire soil nematode populations extracted from bulk soil and during which nitrogen mineralisation was measured. Both undisturbed and disturbed cores were prepared to investigate whether a representative pore structure influences the effect of entire free-living nematode populations on nitrogen mineralisation. Cores were subjected to a 5 kGy gamma irradiation dose sufficient to eliminate all soil fauna while leaving the microbial biomass largely intact. Half of the irradiated cores were reinoculated with nematodes extracted from a corresponding volume of bulk soil and incubated for 82 days. The microbial biomass was not strongly affected by gamma irradiation or nematode addition but declined strongly in all treatments during incubation. Reinoculation of nematodes was successful in establishing populations of a similar size and composition as in the control samples. Net nitrogen mineralisation from indigenous soil organic matter was observed in all treatments throughout the incubation, but was always more pronounced in irradiated cores. Total mineral nitrogen concentrations did not differ significantly between simply irradiated and irradiated then reinoculated cores. However by the end of the incubation period nematode addition resulted in 87% and 23% more NO 3 − –N g −1 dry soil in undisturbed and disturbed cores respectively, while NH 4 + –N g −1 dry soil decreased by 50% in both core types. We found no convincing evidence for a contribution of free-living nematodes on total nitrogen mineralisation, but the activity of nitrifying organisms was clearly stimulated by nematode grazing.

The microbial community (fungi and bacteria) is the main decomposer of organic matter during composting. Its composition is sometimes used as a proxy to assess compost maturity. Although nematodes are probably its most important grazers, only one previous study has highlighted clear shifts in nematode species composition during composting, and the assumption that nematodes reflect changes in the microbial community in compost has not yet been formally tested. Here, the microbial and nematode communities of a single composting process are analyzed together for the first time. Although both displayed broadly similar patterns, the abundance of fungal-feeding nematodes showed a distinct delay as compared to the increase in fungal PLFA. We argue that the nematode community may be a more promising tool to use in assessing compost maturity because it allows to discriminate between the three composting phases. First, during the thermophilic phase, bacterial-feeding nematodes dominate; during cooling, the bacterial-feeding/predators bloom; and during maturation, the abundance of fungal-feeding nematodes increases. Based solely on the microbial community, it was only possible to discriminate between the thermophilic phase and the rest of the process. Bacteria dominated during the thermophilic phase, while bacterial and fungal PLFA had more-or-less equal shares during cooling and maturation.

Biochar produced during pyrolysis of biomass has the potential to reduce greenhouse gas (GHG) emissions from soils. In order to evaluate the effect of four different biochar additions on the emission of the greenhouse gases CO2 and N2O, two incubation experiments were established in a temperate sandy loam soil. Digestate, a waste-product of the wet fermentation of swine manure, and willow wood was slowly pyrolyzed at 350 °C and 700 °C, yielding four biochar types (DS350, DS700, WS350 and WS700). In the first incubation experiment (117 days), C mineralization was monitored in soil amended with biochar at a quantity of 10 Mg ha−1 on an area-basis (biochar to soil ratio of 1:69 on a mass basis) at 50% water filled pore space (WFPS). CO2 emissions from the 350 °C biochar treatments were significantly higher than the control (no biochar) treatment, while we observed no significantly different net C mineralization in the treatments with the 700 °C biochars compared to the control. After fitting a combined zero- plus first-order model to the cumulative C mineralization data, the parameter for the easily mineralizable C pool (CAf) positively correlated with the volatile matter (VM) contents of the biochars. Microbial biomass carbon consistently increased due to all biochar additions, while the dehydrogenase activity increased in the 350 °C biochar treatments but decreased in the 700 °C biochar treatments. Principal component analysis (PCA) of the extracted phospholipid fatty acids (PLFAs) demonstrated that divergent microbial community structures established after the addition of all biochars. The markers for Gram-positive and Gram-negative bacteria were more abundant in the 350 °C biochar treatments compared to the control and to the other biochar treatments. Net N mineralization was higher in the digestate biochar treatments than in the willow biochar treatments and decreased with increasing pyrolysis temperatures and increasing C:N ratio. In a second incubation experiment (15 days) N2O emissions were measured at WFPS of 70% and the same biochars were added in the same quantity as for C mineralization, with the addition of 40 mg KNO3–N kg−1. The cumulative N2O emission after 15 days was positively correlated with the volatile matter content of the biochars and was significantly lower in the 700 °C biochar treatments compared to the control, while no significant differences were found for the 350 °C biochar treatments. This study suggests that volatile matter content could be an important property of biochars in explaining short-term CO2 and N2O emissions from biochar-amended soils.

In Cuba there is a strong differentiation in types of farming systems that operate at very different levels of management intensity. The aim of this research was to characterize differences in soil quality caused by different agricultural management systems and the effects of seasonality on soil chemical and biological indicators within representative farming systems in Santa Clara municipality, Villa Clara Province, Cuba. Two state farms, two cooperative farms, and three private farms, all located on brown calcareous soils, which differed in soil management and technological complexity were selected. Soil samples from two fields of each farm were collected to a depth of 20 cm. Laboratory analysis were performed to determine physical (aggregate stability, plasticity, and permeability), chemical (pH and organic matter), and biological (dehydrogenase and -glucosidase activities) indicators of soil quality. We found significant differences in soil quality among the three farming systems, which were most pronounced between private and state farms, with respect to physical soil properties (aggregate stability and plasticity) and the activity of enzymes. Seasonality also exerted an important influence on the activities of dehydrogenase and -glucosidase, which were greater in the rainy season. As a conclusion, we can say that the differences in soil management between the farming systems were reflected in consistent differences in soil quality indicators, notably between the state and private farms.

A way to bring phosphate-saturated soils back to an environmentally safe P level is by P mining through plants. Phosphate-solubilizing bacteria (PSB) could be very useful for increasing mining efficiency over time. The goal of this research was to investigate the adaptation and performance of PSB in conditions of high total P content in soil. In the first experiment, the P-solubilizing capacity of five PSB species (three Bacillus spp. and two Pseudomonas spp.) were tested under fully controlled conditions on several growth media with different forms of insoluble phosphate (FePO4, AlPO4, or (Ca)3(PO4)2) added at different rates. The colony growth after 14 days of inoculation demonstrated that all five bacteria were able to proliferate and solubilize P on each of the tested growth media, in contradiction with the normally used technique of halo determination. In the second experiment, the same bacterial species were inoculated in pure quartz sand amended with a nutrient solution and P was added separately in an insoluble form, as Fe–P, Al–P, or Ca–P. The extractable ammonium lactate ranged from 3.2 to 6.9 and 29.0 to 40.7 mg kg−1 sand for the insoluble Al–P and Fe–P treatments, respectively. Pseudomonas putida and Bacillus brevis performed best as PSB at high P concentration where the P is fixed with Al or Fe. In the third experiment, P. putida and B. brevis were inoculated in an acidic sandy, P-saturated soil for 4 weeks. The inoculation of the PSB gave promising results in solubilizing P.

The role of soil fauna in crucial ecosystem services such as nutrient cycling remains poorly quantified, mainly because of the overly reductionistic approach adopted in most experimental studies. Given that increasing nitrogen inputs in various ecosystems influence the structure and functioning of soil microbes and the activity of fauna, we aimed to quantify the role of the entire soil nematode community in nutrient mineralization in an experimental set-up emulating nutrient-rich field conditions and accounting for crucial interactions amongst the soil microbial communities and plants. To this end, we reconstructed a complex soil foodweb in mesocosms that comprised largely undisturbed native microflora and the entire nematode community added into defaunated soil, planted with Lolium perenne as a model plant, and amended with fresh grass-clover residues. We determined N and P availability and plant uptake, plant biomass and abundance and structure of the microbial and nematode communities during a three-month incubation. The presence of nematodes significantly increased plant biomass production (+9%), net N (+25%) and net P (+23%) availability compared to their absence, demonstrating that nematodes link below- and above-ground processes, primarily through increasing nutrient availability. The experimental set-up presented allows to realistically quantify the crucial ecosystem services provided by the soil biota.

Separating dairy cattle slurry in a liquid and solid fraction (SF) is gaining more interest, since it enables a more targeted use of both fractions. However, the valorization of the SF is limited on P-rich soils, due to its high P content, and the export or use as bedding material requires sanitation. Therefore, we investigated the influence of composting or ensiling the SF, whether or not mixed with bulking agents, on the product quality in terms of fertilizer value, sanitation and stability. Ensiling can be considered as a controlled storage method for conserving C and nutrients. Soil amendment with co-ensiled SF resulted in a higher N mineralization and crop growth compared to amendment of co-composted SF. Co-composting SF with structure-rich feedstock materials optimized the composting process and sanitation when compared with composting pure SF and did not increase the risk for extreme-heat-resistant spores of thermophilic aerobic spore-forming bacteria (X-TAS). Further, the composts contained more P per unit of fresh weight than the silages, beneficial for the export of the composted SF. The oxygen uptake rate was found to be less powerful to determine the stability of fresh, composted and ensiled SF.

We explored the value of ergosterol, phospholipid fatty acid (PLFA) profiles and neutral lipid fatty acid (NLFA) 16:1ω5c as soil quality indicators for the intensive systems of vegetable production in the humid tropical climate of West Java, by comparing organic and conventional management. Additionally, we measured dehydrogenase and β-glucosidase activity. A secondary forest was included to obtain reference values under undisturbed conditions. Organic and conventional agriculture, and secondary forest each differed in the composition of the microbial community measured by PLFA profiles. Ergosterol appeared not to be universally applicable as a fungal biomarker and in this respect seemed to be inferior compared to PLFA fungal markers. NLFA 16:1ω5c may provide additional information as an indicator of arbuscular mycorrhizal fungi, but its high variability complicated the interpretation of data. The ratio of cy17:0 to 16:1ω7c was effectively applied as an indicator of physiological stress experienced by the bacterial community. Conventional vegetable production resulted in higher cy17:0/16:1ω7c ratios. Finally, a soil quality index, developed by stepwise canonical discriminant analysis and based on the absolute amount of PLFA 16:0, the relative amount of PLFAs 10Me16:0 and 10Me18:0, and dehydrogenase activity, was successfully validated.

The techniques available for sterilisation or defaunation of soil in ecological experiments mostly have strongly unwanted effects on soil structure and the dynamics of major nutrients such as nitrogen. The potential for using gamma irradiation to prepare defaunated soil microcosms was investigated by subjecting undisturbed soil cores to a range of irradiation doses (0, 5, 10, 20 and 40 kGy). The absence of living nematodes at the lowest irradiation dose was confirmed by microscopic observation. The effects of irradiation dose on mineral nitrogen (as NO 3 − and NH 4 + ), microbial biomass C (C mic ), and phospholipid fatty acid (PLFA) concentration and composition were determined over a 4 week incubation period. An increase in the concentration of NO 3 − occurred during the incubation period after exposure to 0, 5 and 10 kGy but was barely detectable at 20 and 40 kGy. The effect of irradiation dose on NH 4 + release was complex and highly variable within treatments, with the 10 kGy dose resulting in the highest concentrations. Microbial biomass carbon was significantly reduced following a 20 kGy irradiation dose and below detection at 40 kGy. Most remarkably, the sum of all measured PLFAs did not differ significantly between most treatments and was not correlated with microbial biomass. In most cases the concentration of signature fatty acids only differed significantly between the control and the highest irradiation dose treatments. To ascertain the sensitivity of microbial taxa to acute gamma irradiation with accuracy, measures of microbial community structure other than PLFA analysis are needed.

Conservation agriculture (CA) characterised by minimal soil disturbance, permanent soil surface cover by dead or living plants and crop rotations is one way of achieving higher soil organic carbon (C) in agricultural fields. Sandy loam and loamy soil samples from zero tillage (ZT) and conventional tillage (CT) plots were taken from farmers’ fields during the dry season in August 2006. Soil organic carbon (SOC) and soil organic nitrogen (SON), microbial biomass carbon (MB-C) and microbial biomass nitrogen (MB-N), C mineralization and SOC distribution in particle size fractions in 0–20 cm layer were evaluated. Forty eight farmers’ fields were randomly sampled at four different locations in Central and Northern Malawi, representing ZT plots maintained for a different number of years, and ten fields under CT with similar soil type and crop grown were selected. SOC and SON in ZT fields were 44 and 41 % (4 years ZT) and 75 and 77 % (5 years ZT) higher, respectively, than CT plots. MB-C and MB-N in ZT fields were 16 and 44 % (4 years ZT) and 20 and 38 % (5 years ZT) higher, respectively, than CT plots. However, MB-C and MB-N in ZT fields were 27 and 25 % (2 years ZT) and 17 and 9 % (3 years ZT) lower than in CT plots. The proportion of the total organic C as microbial biomass C was relatively higher under CT than ZT treatments. The higher SOC and MB-C content in the ZT fields resulted in 10, 62, 57 % higher C mineralization rate in ZT plots of 3, 4 and 5 years of loamy sand soils and 35 % higher C mineralization rate in ZT plot of 2 years than CT of sandy loam soils in undisturbed soils in the laboratory. Simulating plough from the undisturbed soils that were used for C mineralization experiment resulted in linear curves indicating that all organic C was already depleted during the first incubation period. The relative distribution of soil organic matter (SOM) in silt and clay size fractions was strongly correlated (r = 0.907 and P ≤ 0.01) with silt percentages. Easily degradable carbon pool (CA,f) was correlated (r = 0.867 and P ≤ 0.05) with organic carbon in sand size fraction. In developing viable conservation agriculture practices to optimize SOC content and long-term sustainability of maize production systems, priority should be given to the maintenance of C inputs, crop rotations and associations and also to reduced soil disturbance by tillage.

The decomposition and the associated nitrogen (N) dynamics of organic N sources are affected by their contact with soil. While several authors have examined the effect of surface application or incorporation of crop residues on their decomposition rate, less information is available about the relationship between the placement of animal manure and their N mineralization rate. This study investigated the influence of chicken manure and cattle manure placement on soil N mineralization. The manures were incorporated or surface applied at 175 mg N kg−1, and N release was periodically determined over 56 days by measuring inorganic N [nitrate (NO3 −) N and ammonium (NH4 +) N] in a 2 M potassium chloride (KCl) extract at a ratio of 1:10 (w/v). Results indicated that the control soil released a maximum of 64 mg N kg−1 soil at day 21, a sixfold increase over the initial concentration, which indicates its substantial mineralization potential. Manure treatments showed an initial increase in net NO3 −-N content at th...

A change in land use from agriculture to forest generally increases soil acidity. However, it remains unclear to what extent plant traits can enhance or mitigate soil acidification caused by atmospheric deposition. Soil acidification is detrimental for the survival of many species. An in-depth understanding of tree species-specific effects on soil acidification is therefore crucial, particularly in view of the predicted global increases in acidifying nitrogen (N) deposition. Here, we report soil acidification rates in a chronosequence of broadleaved deciduous forests planted on former arable land in Belgium. This region receives one of the highest loads of potentially acidifying atmospheric deposition in Europe, which allowed us to study a ‘worst case scenario’. We show that less than four decades of forest development caused significant soil acidification. Atmospheric deposition undoubtedly and unequivocally drives postagricultural forests towards more acidic conditions, but the rate of soil acidification is also determined by the tree species-specific leaf litter quality and litter decomposition rates. We propose that the intrinsic differences in leaf litter quality among tree species create fundamentally different nutrient cycles within the ecosystem, both directly through the chemical composition of the litter and indirectly through its effects on the size and composition of earthworm communities. Poor leaf litter quality contributes to the absence of a burrowing earthworm community, which retards leaf litter decomposition and, consequently, results in forest-floor build-up and soil acidification. Also nutrient uptake and N2 fixation are causing soil acidification, but were found to be less important. Our results highlight the fact that tree species-specific traits significantly influence the magnitude of human pollution-induced soil acidification.

Nitrogen fertilizer application rates in intensive vegetable production in (South) East Asia have increased exponentially over the past decades, including in the low income countries. While there have been reports of excessive N inputs from e.g. Vietnam, Thailand and Indonesia, very little quantitative knowledge exists on the real extent of the problem. We calculated N balances and agronomic N use efficiencies (ANUE) for a number of typical intensive vegetable rotations in the highlands of Central Java, Indonesia, on fertile Andisols, both for individual cropping cycles (short term) as for 6 consecutive cropping cycles (long term). This was done for farmers practice (FP) treatments, and improved practice (IP) treatments, where N fertilization was significantly reduced. Yields were in general similar in FP and IP, but tended to be slightly higher in IP, with some significant differences. Both the short and long term N balances were always positive and usually very high. Short term N balances ranged from 9 to 559 kg N ha−1 and 219 to 885 kg N ha−1 in IP and FP, respectively, while short term ANUE ranged from 8 to 67 and 4 to 39% in IP and FP, respectively. Long term N balances ranged from 627 to 1,885 kg N ha−1 and 962 to 3,808 kg N ha−1 in IP and FP, respectively, indicating a massive excess of N supply especially in FP. N balances can thus be drastically reduced with no negative impacts on yield, on the contrary. Soil mineral N in the 0–25 cm layer was in general not very high (6.5–38.8 mg N kg−1 soil) and not systematically different between IP and FP, probably as a result of excessive NO3 − leaching. Therefore, topsoil mineral N seems to have only limited indicator value under these conditions. Because denitrification losses in these soils are not very high, most N in excess of the crop requirements will be lost by leaching. Quantitative data on N balances as obtained here may be used to sensitize policy makers and farmers about the threat of current farming practices to the environment, and to improve economic performance.

SUMMARYNitrogen (N) nutrition is a key factor for vegetable growth and yield. However, different rates of nitrogen fertilization may trigger different responses to vegetables. A survey was conducted to investigate the effect of soil fertility management practices on nitrate concentration in vegetables. The survey results were used to plan experiments on the effect of chicken and cattle manures on nitrate levels in Chinese cabbage (Brassica rapa) and amaranthus (Amaranthus cruentus) grown in Tanzania and the patterns of mineral nitrogen in soils under open field conditions. Chicken or cattle manure at 200, 300 kg N ha−1and 170 250 kg N ha−1for Chinese cabbage and amaranthus respectively, and control were compared in a randomized complete block design. We observed a higher nitrate content in fertilized Chinese cabbage at day 30 than at day 44 after sowing, ranging from 3243 to 4993 mg kg−1fresh matter regardless of the N source and rates. Only application of manures at high levels (250 kg N ha−1) induced significantly (p< 0.05) higher nitrate contents in amaranthus at day 28 after sowing, although there was a clear indication of nitrate accumulation even at 170 kg N ha−1application. Soil NH4+-N + NO3−-N in both Chinese cabbage and amaranthus plots were increased with increasing N application rates and differences between control and amended soils were significant (p< 0.01). There was a positive relationship between NO3−concentration in vegetables and NO3−-N in the rooted top soil layer (0–15 cm). However, higher NH4+concentrations depressed NO3−build up in crops and a significant negative relationship between soil (NH4+-N)/(NO3−-N) ratio and crop NO3−content was found. It is concluded that low manure application rates result in similar yields to high rates but reduces nitrate accumulation in vegetables and excess mineral nitrogen in soils.

Rhizoctonia solani causes worldwide losses in numerous crops. Sclerotia of R. solani remain viable for several years in soil and are an important source of primary infection. In this study the effect of soil incorporation of Kraft pine lignin, a side product of the paper industry, on viability of R. solani AG1-1B sclerotia was investigated. The efficacy of lignin was assessed in a sandy loam (Oppuurs) and a silt loam soil (Leest) collected from commercial fields in Belgium. Evaluating sclerotial viability after 4 weeks incubation in the two soils amended with 1% (w/w) Kraft pine lignin demonstrated a soil-dependent effect. In Leest soil the addition of lignin resulted in a significantly reduced sclerotial viability, together with an increased mycoparasitism by Trichoderma spp.; in Oppuurs soil, on the other hand, only a slight and insignificant reduction in sclerotial viability was observed. Based on phospholipid fatty acid analysis, different changes in microbial community structure upon lignin amendment were detected in the two soils. Both amended soils showed a significant increase in Gram negative bacteria. In Leest soil this increase was accompanied with a significantly higher increase in fungi and actinomycetes compared with Oppuurs soil. In addition, Kraft pine lignin resulted in both soils in a small but significant increase in manganese peroxidase activity and this increase tended to be higher in Leest soil. Manganese peroxidase produced by lignin-degrading basidiomycetes has previously been shown to degrade melanin, which protects the sclerotia against biotic and abiotic stress. We hypothesize that lignin-degrading fungi increased the susceptibility of the sclerotia to sclerotial antagonists such as Trichoderma, Gram negative bacteria and actinomycetes. Clearly, the effect observed here did not rely on the stimulation of one microbial group, but is the result of an interaction of different groups.

We compared the effect of organic and conventional farming practices on soil microbial dynamics in West Java, Indonesia. A secondary forest was included to obtain natural reference values. On the organic farms, soil fertility is maintained mainly with composted organic matter in contrast to conventional farmers who combine fresh manure and chemical fertilizers, and typically apply large amounts of pesticides. Parameters measured were dehydrogenase, β-glucosidase, acid phosphomonoesterase and β-glucosaminidase activity, microbial biomass C (MBC) and microbial community composition by phospholipid fatty acid analysis. A strong negative impact of intensive chemical fertilizer and pesticide use on soil enzyme activities was demonstrated. Dehydrogenase and β-glucosidase activities were correlated with soil organic matter content and pH. β-glucosidase activity under organic management approached that under forest, while MBC and dehydrogenase activity remained higher under forest. The composition of the soil microbial community strongly differed between forest and cultivated soil, a clear difference in composition was also observed between conventional and organic farming. Dehydrogenase activity and C16:1ω5c, marker fatty acid for arbuscular mycorrhizal fungi, appeared to be particularly suited as indicators of the impact of management on soil quality and on the soil microbial community.

Sandy cropland soils in NW Europe were found to contain unusually high organic-carbon (OC) levels, and a link with their land-use history has been suggested. This study’s aim was to assess the discriminating power of physical and chemical fractionation procedures to yield information on soil-organic-matter (OM) stability for these soils. In relict- and cultivated-heathland soils, much higher proportions of 6% NaOCl treatment–resistant but 10% HF–soluble OC (MOC) and N (32.2% and 29.9%) were measured compared to a set of “permanent"-cropland soils without a history of heathland land use (11.9% and 8.5%). Also, the proportions of 6% NaOCl– and 10% HF treatment–resistant OC and N in the relict and cultivated heathlands (19.2% and 12.0%) were higher than in the permanent-cropland soils (17.7% and 5.7%). Stepwise multiple linearregression yielded a significant relationship between the annual mineralization (g C [100 g OC]–1), soil OC (g Ckg–1) content, and %MOC: Annual mineralization = 4.347 – 0.087 soil OC – 0.032 %MOC (R2 = 0.65). Combinations of incubation experiments for quantification of the labile soil OM pool with chemical fractionation may thus yield meaningful data for development of soilorganic-matter models with measurable pools, but their applicability will be limited to specific combinations of former land use with soil, climate, and current management.

Crop residues are an important component of N cycling in the agro-ecosystem. In this study, a loam soil was amended with cabbage residues and the N mineralization from soil organic matter and residues, as well as N uptake by Italian rye grass, were investigated. Four mineral fertilizer-residue combination treatments and two application times were tested in micro-plots. For uncropped micro-plots, application of residue resulted in higher soil mineral N content (43 mg N kg−1, 63 days post application) versus 30.5 mg N kg−1 for an unamended soil. Both bulked dry matter production and N uptake by the rye grass decreased in the order full mineral fertilizer > half mineral fertilizer + half residue > full residue applied 3 weeks before sowing > full residue applied at sowing, but the differences were more pronounced in the N uptake. Shoot N recovery from the applied residue (27.5–31.4%) was much smaller than that from the full fertilizer (70.2%), but was still considerable. Apparent net N mineralizatio...

To evaluate the effect of tillage intensity on the N mineralization pattern of winter wheat residues, sugar beet residues, Italian ryegrass and maize residues undisturbed soil samples were taken from six sites under different tillage management. Site NTK had been managed for 10 years under reduced tillage (RT), whereby the last 4 years the crops were sown using direct seeding (NT). Site RTCSE had been managed for 20 years under reduced tillage (RT) and site RTH for 3 years. For each site under RT a nearby site under conventional tillage (CT) was selected (CTK, CTCSE and CTH). On site NTK and site RTCSE a significantly higher amount of SOC in the 0–10 cm was accumulated compared to the respective CT sites. Between site RTH and site CTH no such significant difference was found. However, the content of microbial biomass C (MB-C) and the β-glucosidase and urease activities were higher on all RT sites compared to the respective CT sites. This indicates that these microbiological and biochemical parameters seem to be very sensitive for alterations in management intensity. After 98 days, more N was immobilized under NTK than under CTK by adding winter wheat residues (expressed as kg ha−1 and as % of total added N). This higher immobilization potential can be explained by a higher microbial activity and a change in microbial population. Under RTCSE and RTH net N immobilization of the winter wheat residues was found, but the pattern was less pronounced than for NTK. However, when expressed as % of total N added, N immobilization of winter wheat residues was higher under CT than under RT, which indicates that high C:N residues when incorporated, decompose more slowly under RT than under CT. Similar results were found comparing the N mineralization pattern of maize residues under RTH and CTH. The residues of sugar beet and Italian ryegrass at site CTH released N more rapidly and to a higher extent, 74.1% and 66.2%, respectively (expressed as % of total N added) than under RTH at the end of the incubation. The slower mineralization of N rich crop residues under RT compared to CT means that there is less potential risk for nitrate leaching to occur, which may result in a higher N efficiency in RT compared to CT.

A renewed interest in chemical fractionation of soil organic matter (SOM) originates from the premise that it enables to isolate labile SOM from SOM protected through mineral binding and recalcitrant SOM. Both selective removal of labile non-bound SOM through oxidation or hydrolysis as well as selective removal of minerals and attached SOM are often applied. Molecular-level SOM characterization by means of temperature resolved Pyrolysis-Field Ionization Mass Spectroscopy analysis (Py-FIMS) was used here as an approach to obtain insight into the fate of SOM upon wet chemical treatment with regard to composition and thermal stability. The applied sequential chemical treatment with 6% NaOCl and 10% HF yielded similar sizes in stable SOM fractions between sandy semi-native heathland and cultivated cropland soil pairs (i.e. NaOCl resistant OC: 12.3–15.0 g C kg − 1 and NaOCl + HF resistant OC: 2.6–5.3 g C kg − 1 ). Py-FIMS spectra of bulk SOM in both heathland–cropland soil pairs were dominated by signals assigned to lipids, alkylaromatics and sterols. Difference spectra and thermograms showed selective loss of signals from sterols, lignin dimers and thermolabile lipids. This matches advancing SOM decomposition as derived from previously reported gradients in SOM composition as decomposition proceeds from plant material over particulate organic matter (OM) to SOM in silt and clay particle sizes. However, increased ion intensity attributed to carbohydrates, peptides and short-chained lipids after NaOCl treatment indicates that biologically labile SOM components were also enriched, and they may possibly have been protected through mineral binding or encapsulation in macromolecular OM structures. Subsequent HF treatment yielded increased volatilization in the thermostable region for mass signals tentatively assigned to phenols and lignin monomers and of heterocyclic N-containing compounds and thermostable alkylaromatics. The resistance to chemical treatment of the latter two components matches with their hypothesized structural function in macro OM molecules. However, even for the sites investigated here, with a very similar soil texture, climate, land-use, drainage and contents of pedogenic oxides, contrasting and not readily explainable results were found for other SOM constituents. Therefore, chemical fractionations seem to yield very site specific and less distinct patterns and this study demonstrated that derivation of useful information regarding SOM stabilization mechanisms from such experiments is by no means straightforward.

Total soil organic-carbon (SOC) stocks for grassland soils in Flanders (N Belgium) were determined for the Kyoto Protocol reference year 1990 and 2000 in order to investigate whether these soils have been CO2 sinks or sources during that period. The stocks were calculated by means of detailed SOC datasets, which were available at the community scale for the whole of Flanders. The total SOC stocks for Flemish grassland soils (1 m depth) were estimated at 38 Mt SOC in 1990 and 34 Mt SOC in 2000. The loss of SOC resulted from a decrease in the SOC content of grassland soils (71%) and could also partly (29%) be explained by a decline in grassland area. Significant decreases in %SOC for the 0–6 cm depth layer were found for the 1990s for the coarser-textured soils with SOC losses ranging between –0.3% and –0.5% over the 10 y period. Specific management practices that disturb the SOC balance such as conversion to temporary grassland and a reduction of animal-manure application are hypothesized to have contributed to the observed loss of SOC stocks. We furthermore conducted an analysis of uncertainty of the 1990 and 2000 grassland SOC–stocks calculation using Monte Carlo analysis. Probability-distribution functions were determined for each of the inputs of the SOC-stock calculation, enabling us to assess the uncertainty on the 1990 and 2000 SOC stocks. The frequency distributions of these simulated stocks both closely approached lognormal distributions, and their 95%-confidence intervals ranged between 150% and 50% of the calculated mean SOC stock. The standard error on the measured decrease in SOC stocks in Flemish grassland soils during the 1990s was calculated to be 7–8 Tg SOC, which is equivalent to twice this decrease. This clearly shows that large-scale changes in SOC stocks are uncertainty-ridden, even when they are based on detailed datasets.

Reduced tillage (RT) agriculture is an effective measure to reduce soil loss from soils susceptible to erosion in the short-term and is claimed to increase the soil organic carbon (SOC) stock. The change in distribution and total SOC stock in the 0–60 cm layer, the stratification of microbial biomass carbon (MB-C) content in the 0–40 cm layer and the carbon (C) mineralization in the upper 0–5 cm layer in silt loam soils in Western Europe with different periods of RT agriculture were evaluated. Ten fields at seven locations, representing the important RT types and maintained for a different number of years, and eight fields under conventional tillage (CT) agriculture with similar soil type and crop rotation were selected. RT agriculture resulted in a higher stratification of SOC in the soil profile than CT agriculture. However, the total SOC stock in the 0–60 cm layer was not changed, even after 20 of years RT agriculture. The MB-C was significantly higher in the 0–10 cm layer under RT agriculture, even after only 5 years, compared to CT agriculture. The higher SOC and MB-C content in the upper 0–5 cm layer of RT fields resulted in a higher C mineralization rate in undisturbed soil in the laboratory. Simulating ploughing by disturbing the soil resulted in inconsistent changes (both lower and higher) of C mineralization rates. A crop rotation with root crops, with heavy soil disturbance every 2 or 3 years at harvest, possibly limited the anticipated positive effect of RT agriculture in our research.

ARTICLE I NFO Soil erosion from agricultural lands is a serious problem on the Chinese Loess Plateau. In total, 28 field rainfall simulations were carried on loamy soils under different management practices, namely conventional tillage (CT), no till with mulch (NTM), reduced tillage (RT), subsoiling with mulch (SSM), subsoiling without mulch (SS), and two crops per year (TC), to investigate (i) the effects of different soil management practices on runoff sediment and (ii) the temporal change of runoff discharge rate and sediment concentration under different initial soil moisture conditions (i.e. initially dry soil surface, and wet surface) and rainfall intensity (85 and 170 mm h �1 ) in the Chinese Loess Plateau. NTM was the best alternative in terms of soil erosion control. SSM reduced soil loss by more than 85% in 2002 compared to CT, and its effects on runoff reduction became more pronounced after 4 years consecutive implementation. SS also reduced considerably the runoff and soil loss, but not as pronounced as SSM. TC resulted in a significant runoff reduction (more than 92%) compared to CT in the initial 'dry' soil, but this effect was strongly reduced in the initial 'wet' soil. Temporal change of runoff discharge rate and sediment concentration showed a large variation between the different treatments. In conclusion, NTM is the most favorable tillage practices in terms of soil and water conservation in the Chinese Loess Plateau. SSM can be regarded as a promising measure to improve soil and water conservation considering its beneficial effect on winter wheat yield.

The effect of reduced tillage (RT) on nitrous oxide (N2O) emissions of soils from fields with root crops under a temperate climate was studied. Three silt loam fields under RT agriculture were compared with their respective conventional tillage (CT) field with comparable crop rotation and manure application. Undisturbed soil samples taken in September 2005 and February 2006 were incubated under laboratory conditions for 10 days. The N2O emission of soils taken in September 2005 varied from 50 to 1,095 µg N kg−1 dry soil. The N2O emissions of soils from the RT fields taken in September 2005 were statistically (P

Nutrient dynamics in wetland ecosystems are largely controlled by soil moisture content. Therefore, the influence of soil moisture content on N mineralization should be explicitly taken into account in hydro-ecological models. The aim of this research was to establish relationships between N mineralization and soil moisture content in loamy to silty textured soils of floodplain wetlands in central Belgium. Large undisturbed soil cores were taken, incubated for 3 months under various moisture contents, and zero order and first order N mineralization rates were calculated. We used the percentage water-filled pore space (WFPS) as an expression of soil moisture because it is a better index for aeration dependent biological processes than volumetric moisture content or water retention. The relationship between the N mineralization rate and %WFPS was described by a Gaussian model. The optimum WFPS for N mineralization ranged between 57% and 78%, with a mean of 65% ± 6% WFPS. Expected annual net N mineralization rates at field temperature (9.7°C) and at optimal moisture content varied between 30 and 186 kg N ha-1 (0–15 cm depth) year-1, with a mean of 110 ± 42 kg N ha-1 (0–15 cm) year-1. The mean N turnover rate amounted to 2.3 ± 1.1 g N 100 g-1 N year-1. Multiple linear regressions between N mineralization and general soil parameters showed that soil structure has an overriding impact on N mineralization in wetland ecosystems.

Unusually high SOC levels have been reported for sandy cropland soils in North-Western Europe. A potential link with their general heathland land-use history was investigated by comparing two soil pairs of relict heathland and cultivated former heathland in the Belgian sandy region. A sequential chemical fractionation yielded similar sizes in corresponding SOM fractions between the heathland and cropland soils (i.e. NaOCl resistant: 12.3–15.0 g C kg−1 and NaOCl + HF resistant: 2.6–5.3 g C kg−1). Higher amounts of clay sized N in the cropland plots can be attributed to N additions from mineral fertilizers and animal manure. Temperature resolved Pyrolysis Field Ionization Mass Spectroscopy analysis showed that the composition of both relict heathland and cultivated soils was surprisingly similar, in spite of over 60 years of intense cropland management. The mass spectra of SOM in both heathland-cropland soil pairs investigated was dominated by signals from lipids, alkylaromatics and sterols. The accumulation of this SOM rich in aliphatics was logically linked to the high input of lipids, long-chain aliphatics and sterols from heathland vegetation and the low soil pH and microbial activity. Based on the relatively high OC surface loadings of HF-extractable OM (13–44 mg C m−2 Fe and 1.2–2.3 mg C m−2 clay), direct organo-mineral bonds between OM and Fe-oxides or clay minerals seem to be only partly involved as a stabilization mechanism in these soils. The distinct bimodal shape of the thermograms indicates that OM-crosslinking could furthermore contribute substantially to SOM stabilization in these soils. This study therefore corroborates the previously proposed view that lipids may be bound in networks of alkylaromatics, the structural building blocks of OM macromolecules. We hypothesize that such binding is able to explain the measured retention of these OM components, even under several decades of cropland management.

In the scope of the increasing concern for soil conservation, reduced tillage (RT) agriculture is growing more important in today’s agriculture in Western Europe. However, crop rotations often include beets and potatoes, crops that are generally assumed to be less suitable under RT agriculture because they result in a high disturbance of the soil at the formation of the ridges and at harvest. Therefore, the short- and long-term effect of RT agriculture on bulk density (BD), water retention curve (WRC), aggregate stability and field-saturated hydraulic conductivity of silt loam soils with crop rotations including root crops was evaluated. Ten fields at seven locations representing the important RT types, applied for a different number of years, and eight fields under conventional tillage (CT) agriculture with similar soil type and crop rotation were selected. At each location, BD of the 5–10 cm layer was mostly lower in the RT fields (1.42 � 0.05 Mg m � 3 [average with

Crop rotations in Western Europe contain crops that seem not suitable for reduced tillage (RT) agriculture because they often include beets and potatoes, resulting in a high disturbance of the soil at the formation of the ridges and at harvest. Therefore, the short- and long-term effects of RT on the stratification and stock of total nitrogen (TN) in the 0–40 cm depth layer and the nitrogen (N) mineralization in the upper 15 cm depth layer of silt loam soils was evaluated. For doing so, 10 fields at seven locations representing the important types of RT systems in Belgium, applied for a different number of years, and eight fields under conventional tillage (CT) with comparable soil type and crop rotation were selected. Despite the presence of root and tuber crops in these rotations, the stratification of the percentage of TN and of the carbon to nitrogen ratio was more pronounced under RT than CT fields. The TN stock in the RT fields worked with a cultivator or soil loosener was comparable to CT fields, even after 20 years. No trend could be found in the change in TN stock of reduced tillage by direct drilling compared to CT. The N mineralization rate in undisturbed soil cores under controlled conditions in the laboratory was on average 0.20 ± 0.08 mg N kg −1 dry soil day −1 for RT fields compared to an average of 0.13 ± 0.05 mg N kg −1 dry soil day −1 for CT fields. This increase in N mineralization rate was correlated with a higher microbial biomass nitrogen content.

Because the widely used ceramic cups are reported to release, adsorb, and filter dissolved nutrients from soil solution samples, inert cups made of poly(tetrafluoroethene) (PTFE) are frequently proposed as a more reliable alternative. A ceramic and a PTFE/quartz cup were compared with each other in this laboratory study. The PTFE/quartz cup was the most suited for pH measurements. Because only the ceramic cup samples both ammonium and nitrate in a satisfactory way, this cup type is recommended for nitrogen (N) balance studies. In most cases, the ceramic cup also performed better in sampling cations [sodium (Na+), potassium (K+), calcium (Ca2+), magnesium (Mg2+)] than the PTFE/quartz cup. Both cup types seemed inadequate to sample soil solution for aluminum (Al3+) determinations. The ceramic cups released chloride (Cl−) at low ion concentrations, but sulfate (SO4 2−) concentrations remained almost unaltered in the sample as compared to the solution outside the cup. In contrast, Cl− and SO4 2− were...

Laboratory incubation experiments were conducted to study the C and N mineralization dynamics of crop residues (fine roots and straw) of the two main crops (winter wheat and peanut) in the Chinese Loess Plateau under different ways of incorporation. The C mineralization patterns of the soil amended with winter wheat residues differed greatly, and the highest C mineralization was observed in the treatment with winter wheat straw incorporated (39% of the total added C mineralized). The way of straw placement had only a minor effect on the pattern of C mineralization for peanut. Generally, winter wheat residues showed a stronger immobilization than peanut residues during the incubation period, without any net N release. Winter wheat straw incorporated showed the strongest N immobilization with 35 mg kg−1 (equivalent to 27% of added N) immobilized at the eighth week. This study indicated that retaining crop residues at the soil surface in the dry land soils of the Chinese Loess Plateau is beneficial for C sequestration. It also showed that N immobilization occurs only during a limited period of time, sufficient to prevent part of the mineral N pool from leaching, and that net N mineralization can be expected during the subsequent cropping season, thus enhancing synchronization of N supply and demand.

Compost and other organic amendments have been proven to improve the soil physical quality. In Flanders, vegetable, fruit and garden (VFG) wastes are collected selectively and composted. We studied the effects of the combined application of three different doses of VFG compost and cattle slurry and one treatment with only mineral N applied, during 9 years on a range of soil physical properties: aggregate stability, saturated hydraulic conductivity, bulk density, total pore volume and soil moisture retention. The organic amendments had a significantly beneficial impact (p

The effect of straw and vinasses on the N mineralization–immobilization turnover of celery residues was investigated in a field experiment in Flanders (Belgium). The field was laid out in raised beds on a loamy sand soil. At the start of the experiment, 15 N-labelled celery residues (4 t dry matter (DM) ha −1 ) were mixed with straw (12 t DM ha −1 ) in order to immobilize the released celery-N followed by an incorporation of vinasses (4 t DM ha −1 ) after 200 days aiming to remineralize the immobilized N. Total N, mineral N and their 15 N enrichments as well as microbial biomass N were determined at regular time intervals. During the first 62 days after the incorporation, straw immobilized the celery derived 15 N in the microbial biomass and reduced the total celery- 15 N losses from the top 25 cm by 38%. However, after 62 days, the N immobilization ceased due to low temperatures ( 15 N (without addition of vinasses) at a moment when the risk of nitrate leaching was still high. Hence, straw was not able to reduce the celery-N losses during the complete winter period. The addition of vinasses in spring caused no real positive priming effect, although it did increase the amount of remineralized celery- 15 N (7.6% of celery derived 15 N) compared to the straw treatment without vinasses (1.7% of celery derived 15 N) probably due to an apparent added nitrogen interaction caused by displacement reactions with the soil microbial biomass. In conclusion, in raised beds, it is not possible to reduce nitrate leaching and to achieve a synchronization between the N release from crop residues and N demand by the following crop by using straw and vinasses to manipulate the N release from crop residues.

On a field experiment, started in spring 2005, we examined the influence of different types or qualities of exogenous organic matter (farmyard manure, cattle slurry and three different types of compost, compared with mineral fertilization, and two unfertilized treatments, cropped and uncropped) on the earthworm density and biomass. Earthworms were sampled with mustard powder and subsequent handsorting at the start of the experiment and one year (i.e. two organic matter applications) later. The organic matter applications had a significant effect on the earthworm populations. Earthworm abundance was highest on all organically amended plots, while the lowest number and biomass was found on plots without fertilization. On the latter earthworm number and biomass were equal to or lower than the initial situation. The highest earthworm biomass was recorded on plots amended with farmyard manure. There were indications too that the presence of a crop and the crop performance have an effect on the earthworm populations.