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51. Flexible tethering of primase and DNA Pol α in the eukaryotic primosome

Author: Mairi L. Kilkenny, Eva Nogales, Rafael Núñez-Ramírez, Luca Pellegrini, Oscar Llorca, Rajika L. Perera, Begoña García-Alvarez, Sebastian Klinge, Roberto Melero, María Ángeles Recuero-Checa, Richard Hall, Ludovic Sauguet, Centro de Investigaciones Biológicas (CSIC), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), University of Cambridge [UK] (CAM), University of California, Spanish Ministry of Science and Innovation (SAF2008-00451 to O.L.), the ‘Red Temática de Investigación Cooperativa en Cáncer (RTICC)’ from the ‘Instituto de Salud Carlos III’ (RD06/0020/1001 to O.L.), the Human Frontiers Science Program (RGP39/2008 to O.L. and E.N.), a Wellcome Trust Senior Fellowship award in Basic Biomedical Sciences (to L.P.), a FPI fellowship from the Spanish Ministry of Science and Innovation to MAR-C, a JAE-DOC contract of the ‘Consejo Superior de Investigaciones Científicas (CSIC)’ and a ‘Juan de la Cierva’ contract from the Spanish Ministry of Science to BGA, EN is a Howard Hughes Medical Institute Investigator. Funding for open access charge: Spanish Ministry of Science and Innovation (SAF2008-00451 to O.L.)., Kilkenny, Mairi [0000-0002-7280-8522], Pellegrini, Luca [0000-0002-9300-497X], and Apollo - University of Cambridge Repository
Subjects: Models, Molecular, dnaI, MESH: Amino Acid Sequence, chemistry.chemical_compound, Models, Structural Biology, Polymerase, Genetics, 0303 health sciences, biology, 030302 biochemistry & molecular biology, Biological Sciences, MESH: Protein Subunits, MESH: Saccharomyces cerevisiae, 3. Good health, Cell biology, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], MESH: DNA Primase, Primase, Corrigendum, MESH: Models, Molecular, [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], Saccharomyces cerevisiae, Molecular Sequence Data, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, DNA Primase, Primosome, 03 medical and health sciences, MESH: RNA, Information and Computing Sciences, [CHIM.CRIS]Chemical Sciences/Cristallography, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Amino Acid Sequence, 030304 developmental biology, MESH: DNA Polymerase I, MESH: Molecular Sequence Data, Molecular, biology.organism_classification, DNA Polymerase I, Protein Subunits, chemistry, biology.protein, RNA, Primer (molecular biology), DNA polymerase I, [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM], Environmental Sciences, DNA, Developmental Biology
Abstract: 13 páginas, 7 figuras -- PAGS nros. 8187-8199, Núñez-Ramírez, Rafael et al., The Pol α/primase complex or primosome is the primase/polymerase complex that initiates nucleic acid synthesis during eukaryotic replication. Within the primosome, the primase synthesizes short RNA primers that undergo limited extension by Pol α. The resulting RNA–DNA primers are utilized by Pol δ and Pol ε for processive elongation on the lagging and leading strands, respectively. Despite its importance, the mechanism of RNA–DNA primer synthesis remains poorly understood. Here, we describe a structural model of the yeast primosome based on electron microscopy and functional studies. The 3D architecture of the primosome reveals an asymmetric, dumbbell-shaped particle. The catalytic centers of primase and Pol α reside in separate lobes of high relative mobility. The flexible tethering of the primosome lobes increases the efficiency of primer transfer between primase and Pol α. The physical organization of the primosome suggests that a concerted mechanism of primer hand-off between primase and Pol α would involve coordinated movements of the primosome lobes., The first three-dimensional map of the eukaryotic primosome at 25 Å resolution provides an essential structural template for understanding initiation of eukaryotic replication, Spanish Ministry of Science and Innovation (SAF2008-00451 to O.L.); the ‘Red Temática de Investigación Cooperativa en Cáncer (RTICC)’ from the ‘Instituto de Salud Carlos III’ (RD06/0020/1001 to O.L.); the Human Frontiers Science Program (RGP39/2008 to O.L. and E.N.); a Wellcome Trust Senior Fellowship award in Basic Biomedical Sciences (to L.P.); a FPI fellowship from the Spanish Ministry of Science and Innovation to MAR-C; a JAE-DOC contract of the ‘Consejo Superior de Investigaciones Científicas (CSIC)’ and a ‘Juan de la Cierva’ contract from the Spanish Ministry of Science to BGA; EN is a Howard Hughes Medical Institute Investigator. Funding for open access charge: Spanish Ministry of Science and Innovation (SAF2008-00451 to O.L.)
Published: 2011
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52. The RNA-binding protein HuR regulates DNA methylation through stabilization of DNMT3b mRNA

Author: Myriam Gorospe, Rocío G. Urdinguio, Mario F. Fraga, Manel Esteller, Filipe V. Jacinto, Kotb Abdelmohsen, Subramanya Srikantan, María Berdasco, Isabel López de Silanes, Hiroaki Taniguchi, Miguel Alaminos, [Lopez de Silanes,I, Taniguchi,H, Berdasco,M, Urdinguio,RG, Fraga,MF, Jacinto,FV, Esteller M] Cancer Epigenetics Laboratory, Molecular Pathology Program, Spanish National Cancer Research Centre (CNIO), Madrid, Spain. [Gorospe, M, Abdelmohsen K] Laboratory of Cellular and Molecular Biology, Intramural Research Program, National Institute on Aging, National Institutes of Health, Baltimore, USA. [Alaminos,M ] Department of Histology, Granada University and Hospital Clinico Foundation, Granada. [Esteller,M] Institucio Catalana de Recerca i Estudis Avanc¸ ats (ICREA), Barcelona. [Esteller,M] Cancer Epigenetics and Biology Program (PEBC), Catalan Institute of Oncology (ICO), Institut d’Investigacio Biomedica de Bellvitge (IDIBELL), L’Hospitalet, Barcelona, Catalonia, Spain., Grants SAF2007-00027-65134, Consolider CSD2006-49, CANCERDIP FP7-200620, and Spanish Ramon & Cajal Programme and the FIS Programme (PI061653) both from the Spanish Ministry of Science and Innovation (to I.L.S.). National Institute on Aging-IRP, National Institutes of Health (to M.G. and K.A.). Funding for open access charge: Fondo de Investigaciones Sanitarias (FIS). Spanish Ministry of Science and Innovation.
Subjects: Anatomy::Cells::Cells, Cultured::Cell Line::Cell Line, Tumor [Medical Subject Headings], RNA Stability, Chemicals and Drugs::Inorganic Chemicals::Chlorine Compounds::Cisplatin [Medical Subject Headings], Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::RNA Stability [Medical Subject Headings], ADN, RNA-binding protein, ELAV-Like Protein 1, Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings], 0302 clinical medicine, DNA (Cytosine-5-)-Methyltransferases, Antígenos de Superficie, Ribonucleoprotein, 0303 health sciences, Tumor, RNA-Binding Proteins, Cisplatino, Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Nucleoproteins::RNA-Binding Proteins [Medical Subject Headings], Methylation, Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Transferases::One-Carbon Group Transferases::Methyltransferases::Protein Methyltransferases [Medical Subject Headings], 3. Good health, Surface, ELAV Proteins, 030220 oncology & carcinogenesis, Antigens, Surface, ADN (Citosina-5-) Metiltransferasa, Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::DNA Methylation [Medical Subject Headings], embryonic structures, DNA methylation, Metilación de ADN, Datos de Secuencia Molecular, Molecular Sequence Data, Estabilidad del ARN, Antineoplastic Agents, Biology, DNA methyltransferase, 03 medical and health sciences, Molecular sequence data, Cell Line, Tumor, Genetics, Humans, RNA, Messenger, Antigens, 030304 developmental biology, AU-rich element, Messenger RNA, Base Sequence, Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Antineoplastic Agents [Medical Subject Headings], RNA, DNA, DNA Methylation, Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::RNA::RNA, Messenger [Medical Subject Headings], Molecular biology, Hu, Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Molecular Structure::Base Sequence [Medical Subject Headings], Information Science::Information Science::Information Services::Documentation::Molecular Sequence Data [Medical Subject Headings], Línea Celular Tumoral, Chemicals and Drugs::Biological Factors::Antigens::Antigens, Surface [Medical Subject Headings], Cisplatin, Cell line, Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Transferases::One-Carbon Group Transferases::Methyltransferases::DNA Modification Methylases::DNA-Cytosine Methylases::DNA (Cytosine-5-)-Methyltransferase [Medical Subject Headings], Secuencia de Bases
Abstract: The molecular basis underlying the aberrant DNA-methylation patterns in human cancer is largely unknown. Altered DNA methyltransferase (DNMT) activity is believed to contribute, as DNMT expression levels increase during tumorigenesis. Here, we present evidence that the expression of DNMT3b is post-transcriptionally regulated by HuR, an RNA-binding protein that stabilizes and/or modulates the translation of target mRNAs. The presence of a putative HuR-recognition motif in the DNMT3b 3′UTR prompted studies to investigate if this transcript associated with HuR. The interaction between HuR and DNMT3b mRNA was studied by immunoprecipitation of endogenous HuR ribonucleoprotein complexes followed by RT–qPCR detection of DNMT3b mRNA, and by in vitro pulldown of biotinylated DNMT3b RNAs followed by western blotting detection of HuR. These studies revealed that binding of HuR stabilized the DNMT3b mRNA and increased DNMT3b expression. Unexpectedly, cisplatin treatment triggered the dissociation of the [HuR-DNMT3b mRNA] complex, in turn promoting DNMT3b mRNA decay, decreasing DNMT3b abundance, and lowering the methylation of repeated sequences and global DNA methylation. In summary, our data identify DNMT3b mRNA as a novel HuR target, present evidence that HuR affects DNMT3b expression levels post-transcriptionally, and reveal the functional consequences of the HuR-regulated DNMT3b upon DNA methylation patterns., Grants SAF2007-00027-65134; Consolider CSD2006-49; CANCERDIP FP7-200620; Spanish Ramon & Cajal Programme and the FIS Programme (PI061653) both from the Spanish Ministry of Science and Innovation (to I.L.S.). National Institute on Aging-IRP, National Institutes of Health (to M.G. and K.A.). Funding for open access charge: Fondo de Investigaciones Sanitarias (FIS). Spanish Ministry of Science and Innovation.
Published: 2009
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53. Prenatal exposure to multiple persistent organic pollutants in association with adiposity markers and blood pressure in preadolescents

Author: Rouxel, Elke, Costet, Nathalie, Monfort, Christine, Audouze, Karine, Cirugeda, Lourdes, Gaudreau, Éric, Grimalt, Joan O., Ibarluzea, Jesus, Lainé, Fabrice, Llop, Sabrina, Lopez-Espinosa, Maria Jose, Rouget, Florence, Santa-Marina, Loreto, Vrijheid, Martine, Chevrier, Cécile, Casas, Maribel, Warembourg, Charline, Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), École des Hautes Études en Santé Publique [EHESP] (EHESP), Toxicité environnementale, cibles thérapeutiques, signalisation cellulaire (T3S - UMR_S 1124), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Instituto de Salud Carlos III [Madrid, Spain] (ISCIII), Universitat Pompeu Fabra [Barcelona] (UPF), Institut National de Santé Publique du Québec [Canada] (INSPQ), University of the Basque Country/Euskal Herriko Unibertsitatea (UPV/EHU), Centre d'Investigation Clinique [Rennes] (CIC), Université de Rennes (UR)-Hôpital Pontchaillou-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Pontchaillou [Rennes], Universitat Jaume I, Universitat de València (UV), This study received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 825712 – the OBERON project, from the French Agency for Environmental Health Safety (ANSES) under grant agreement No EST19-233, and from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 874583, the ATHLETE project. This publication reflects only the authors’ view, and funding agencies are not responsible for any use that may be made of the information it contains. PELAGIE cohort: This study has been funded by Inserm (since the beginning), the French Ministries of Health (2003–2004), Labor (2002–2003), and Research (ATC 2003–2004), the French National Institute for Public Health Surveillance (InVS, 2002–2006), the National Agency for Research (ANR, 2005–2008, 2010–2012, 2015–2019), the French Agency for Environmental Health Safety (Afsset/ANSES, 2007–2009, 2009–2012), the French Agency for Drug Safety (2013–2017), the Fondation de France (2014–2017, 2015–2018, 2017–2021), the French Ministry of Ecology (PNRPE 2014–2016) and the Research Institute of Public Health (IResP 2011–2014), as well as the following European programs: Hi-WATE 2007–2009, and ENRIECO 2008–2010. INMA-Gipuzkoa: This study was funded by grants from Instituto de Salud Carlos III (FIS-PI13/02187 and FIS-PI18/01142 incl. FEDER funds), CIBERESP, Department of Health of the Basque Government (2015111065), and the Provincial Government of Gipuzkoa (DFG15/221) and annual agreements with the municipalities of the study area (Zumarraga, Urretxu, Legazpi, Azkoitia y Azpeitia y Beasain). INMA-Sabadell: This study was funded by grants from Instituto de Salud Carlos III (Red INMA G03/176, CB06/02/0041, PI041436, PI081151 incl. FEDER funds), CIBERESP, Generalitat de Catalunya-CIRIT 1999SGR 00241, Generalitat de Catalunya-AGAUR 2009 SGR 501, and Fundació La Marató de TV3 (090430). ISGlobal acknowledges support from the Spanish Ministry of Science and Innovation through the 'Centro de Excelencia Severo Ochoa 2019–2023' Program (2018–000806-S) and support from the Generalitat de Catalunya through the CERCA Program. INMA-Valencia: This study was funded by grants from the EU (FP7-ENV-2011 cod 282957, HEALTH.2010.2.4.5–1, and H2020 No 874583, the ATHLETE project), Spain: ISCIII (G03/176, FIS-FEDER: PI11/01007, PI11/02591, PI11/02038, PI12/00610, PI13/1944, PI13/2032, PI14/00891, PI14/01687, PI16/1288, PI17/00663 and PI19/1338, and Miguel Servet-FEDER MS11/00178, MS15/00025, MS20/0006,and MS II16/00051), Generalitat Valenciana (CIAICO/2021/132), FISABIO (UGP 15–230, UGP-15–244, and UGP-15–249), and Alicia Koplowitz Foundation 2017.
Subjects: [SDV]Life Sciences [q-bio], Blood pressure, Mixture, Chemicals, Birth cohort, Body mass index, Persistent organic pollutant
Abstract: International audience; BACKGROUND: Several studies have reported that prenatal exposure to some persistent organic pollutants (POPs) is associated with higher adiposity in childhood. Few studies have assessed whether this finding persists into adolescence, and few have considered exposure to POPs as a mixture. This study aims to assess the association between prenatal exposure to multiple POPs and adiposity markers and blood pressure in preadolescents. METHODS: This study included 1667 mother-child pairs enrolled in the PELAGIE (France) and the INMA (Spain) mother-child cohorts. Three polychlorobiphenyls (PCB 138, 153 and 180, treated as a sum of PCBs) and three organochlorine pesticides (p,p’-Dichlorodiphenyldichloroethylene [p,p’-DDE], β-hexachlorocyclohexane [β-HCH], and hexachlorobenzene [HCB]) were assessed in maternal or cord serum. Body mass index z-score (zBMI), abdominal obesity (waist-to-height ratio > 0.5), percentage of fat mass, and blood pressure (mmHg) were measured at around 12 years of age. Single-exposure associations were studied using linear or logistic regressions, and the POP mixture effect was evaluated using quantile G-computation (qgComp) and Bayesian Kernel Machine Regression (BKMR). All models were adjusted for potential confounders and performed for boys and girls together and separately. RESULTS: Prenatal exposure to the POP mixture was associated with higher zBMI (beta [95 % CI] of the qgComp = 0.15 [0.07; 0.24]) and percentage of fat mass (0.83 [0.31; 1.35]), with no evidence of sex-specific association. These mixture effects were also statistically significant using BKMR. These associations were driven mainly by exposure to HCB and, to a lesser extent, to β-HCH. In addition, the single-exposure models showed an association between β-HCH and p,p’-DDE and higher systolic blood pressure, especially in girls (p,p’-DDE for girls = 1.00 [0.15; 1.86]). No significant associations were found for PCBs. CONCLUSION: This study suggests that prenatal exposure to POPs, particularly organochlorine pesticides, remains associated with unfavorable cardiometabolic health up to the age of 12.
Published: 2023
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54. Robust coalitional model predictive control with plug-and-play capabilities

Author: Masero Rubio, Eva, Baldivieso-Monasterios, Pablo R., Maestre Torreblanca, José María, Trodden, Paul A., Universidad de Sevilla. Departamento de Ingeniería de Sistemas y Automática, Universidad de Sevilla. TEP116: Automática y Robótica Industrial, European Research Council for the Advanced Research grant OCONTSOLAR ref. 789051, MCIN/AEI/ 10.13039/501100011033 grant PID2020-119476RB-I00, and Spanish Ministry of Science and Innovation grant FPU18/04476
Subjects: Tubes, Control by clustering, Distributed networked systems, Model predictive control, Plug-and-play events
Abstract: This article presents a distributed implementation of a model predictive controller with information exchange to manage a distributed networked system of coupled dynamic subsystems. We propose a coalitional control method, where local controllers coalesce into clusters to improve performance, as a tool to solve plug-and-play problems. Our main contribution is a tube-based coalitional approach that employs online optimized invariant sets. These sets are instrumental in guaranteeing recursive feasibility and stability when faced with plug-and-play operations, i.e., subsystems joining or leaving the network. We also explore the inherent robustness properties to absorb disturbances not covered by the tubes without the need to group local controllers. Finally, the simulation results show the benefits of our proposed control method.
Published: 2023

55. Modelling coarse-sediment propagation following gravel augmentation: The case of the Rhône River at Péage-de-Roussillon (France)

Author: Vázquez-Tarrío, Daniel, Peeters, Alexandre, Cassel, Mathieu, Piégay, Hervé, Environnement, Ville, Société (EVS), École normale supérieure de Lyon (ENS de Lyon)-École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université Lumière - Lyon 2 (UL2)-Université Jean Moulin - Lyon 3 (UJML), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Jean Monnet - Saint-Étienne (UJM)-École Nationale des Travaux Publics de l'État (ENTPE)-École nationale supérieure d'architecture de Lyon (ENSAL)-Centre National de la Recherche Scientifique (CNRS), Agence de l'eau Rhône Méditerranée Corse, CNR, EDF, Region Auvergne-Rhône-Alpes, Region PACA, Region Occitanie, Spanish Ministry of Science and Innovation, ANR-17-EURE-0018,H2O'LYON,School of Integrated Watershed Sciences(2017), and ANR-11-LABX-0010,DRIIHM / IRDHEI,Dispositif de recherche interdisciplinaire sur les Interactions Hommes-Milieux(2011)
Subjects: History, Hidrología, Geodinámica, Polymers and Plastics, Sediment pulse, Bedload, Geografía física, Gravel augmentation, Sediment transport, Industrial and Manufacturing Engineering, Gravel-bed rivers, [SDE]Environmental Sciences, Geología, Business and International Management, Earth-Surface Processes
Abstract: International audience; Over the last two centuries, rivers worldwide have been affected by human interventions, including dams, river training, and gravel mining. Such actions have often involved river fragmentation and sediment starvation. In this regard, gravel augmentation is an increasingly common restoration practice for mitigating sediment starvation in gravel-bed rivers. However, uncertainties remain on how to better implement and design such operations. This is the case for the Rhône River at Péage-de-Roussillon, France, where 6885 m3 of gravels were augmented in 2017. This work raised some concerns from river managers about the potential threat posed to a downstream reservoir from the arrival of the sediment, and explains why they were interested in the timeframe of sediment propagation, and travel distances and velocities of the augmented sediment. To answer these questions, we propose a modelling framework for simulating the long-term downstream propagation of a pulse of augmented sediment, with this framework being based on a combination of particle tracking data collected in the field and bedload transport capacity estimates. This workflow allowed us to successfully model propagation of gravels along the study reach, and provided useful information on how the sediment wave would behave over the long term. We believe that the methodology proposed in this study has much potential for exploring and investigating the kinematics of sediment wave propagation in gravel-bed rivers.
Published: 2023
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56. A holistic model-less approach for the optimal real-time control of power electronics-dominated AC microgrids

Author: Olives Camps, Juan Carlos, Rodríguez del Nozal, Álvaro, Mauricio Ferramola, Juan Manuel, Maza Ortega, José María, Universidad de Sevilla. Departamento de Ingeniería Eléctrica, Universidad de Sevilla. TEP196: Sistemas de Energía Eléctrica, Universidad de Sevilla, Spain in the framework of VI PPIT-US, Spanish Ministry of Science and Innovation under grant no. PID2021-124571OB-I00, Junta de Andalucía, Spain under the project FLEX-REN (P18-TP-3655), CERVERA research programme of CDTI, Spain, the Industrial and Technological Development Centre of Spain, under the research Project HySGrid+ (CER-20191019), and H2020 under SUNRISE project grant agreement 101079200
Subjects: Hierarchical control, Secondary voltage control, AC microgrids, Load sharing control, Online feedback optimization
Abstract: This paper addresses the problem of optimally operating a set of grid-forming devices in an AC microgrid when a detailed network model is not available. The main aim of the approach is to maximize the power sharing of the controllable grid-forming devices and to maintain the frequency and the nodal voltages of the microgrid as close as possible to their corresponding references. The proposed control architecture is conformed by a local control layer in each grid-forming device that intends to emulate the performance of a synchronous machine and a centralized secondary controller composed of two complementary tools that coordinates the setpoints of the grid-forming devices: an online feedback optimization algorithm and an automatic generation control. The proposed method has been validated through simulations and hardware-in-the-loop tests, evidencing its good performance and robustness under different conditions.
Published: 2023

57. Robust In Vitro and In Vivo Immunosuppressive and Anti-inflammatory Properties of Inducible Caspase-9-mediated Apoptotic Mesenchymal Stromal/Stem Cell

Author: Alejandra Romecin, Paola, Vinyoles, Meritxell, Lopez-Millan, Belen, Diaz de la Guardia, Rafael, Atucha, Noemi M., Querol, Sergi, Bueno, Clara, Benitez, Raquel, Gonzalez-Rey, Elena, Delgado, Mario, Menendez, Pablo, [Alejandra Romecin, Paola] Josep Carreras Leukemia Res Inst, Barcelona, Spain, [Vinyoles, Meritxell] Josep Carreras Leukemia Res Inst, Barcelona, Spain, [Lopez-Millan, Belen] Josep Carreras Leukemia Res Inst, Barcelona, Spain, [Bueno, Clara] Josep Carreras Leukemia Res Inst, Barcelona, Spain, [Menendez, Pablo] Josep Carreras Leukemia Res Inst, Barcelona, Spain, [Alejandra Romecin, Paola] ISCIII, RICORS TERAV, Madrid, Spain, [Lopez-Millan, Belen] ISCIII, RICORS TERAV, Madrid, Spain, [Querol, Sergi] ISCIII, RICORS TERAV, Madrid, Spain, [Bueno, Clara] ISCIII, RICORS TERAV, Madrid, Spain, [Menendez, Pablo] ISCIII, RICORS TERAV, Madrid, Spain, [Lopez-Millan, Belen] Univ Granada, GENYO, Ctr Pfizer, Junta Andalucia Genom & Invest Oncol, Granada, Spain, [Diaz de la Guardia, Rafael] Univ Granada, GENYO, Ctr Pfizer, Junta Andalucia Genom & Invest Oncol, Granada, Spain, [Atucha, Noemi M.] Fac Med, Dept Fisiol Humana, Murcia, Spain, [Querol, Sergi] Banc Sang & Teixits, Barcelona, Spain, [Bueno, Clara] ISCIII, CIBERONC, Barcelona, Spain, [Menendez, Pablo] ISCIII, CIBERONC, Barcelona, Spain, [Benitez, Raquel] Inst Parasitol & Biomed Lopez Neyra IPBLN CSIC, Granada, Spain, [Gonzalez-Rey, Elena] Inst Parasitol & Biomed Lopez Neyra IPBLN CSIC, Granada, Spain, [Delgado, Mario] Inst Parasitol & Biomed Lopez Neyra IPBLN CSIC, Granada, Spain, [Menendez, Pablo] Inst Catalana Recerca & Estudis Avancats ICREA, Barcelona, Spain, Health Canada, Juan de la Cierva fellowship by the Spanish Ministry of Science and Innovation, and Spanish Association of Cancer Research (AECC)
Subjects: iCasp9 switch, Protein, Anti-Inflammatory Agents, Mesenchymal Stem Cells, Cell Biology, General Medicine, Safety switch, BM-MSC, Experimental colitis, Caspase 9, Immunomodulation, Extracellular Vesicles, Colitis in vivo model, Improve, Humans, WJ-MSC, Anti-inflammatory, Immunosuppressive Agents, Immunosuppression, Developmental Biology
Abstract: The financial support for this work was obtained from Health Canada (H4080-144541) to P.M. M.V. and B.L.-M. were supported by a Juan de la Cierva fellowship by the Spanish Ministry of Science and Innovation (IJCI-2017-3317) and a fellowship from the Spanish Association of Cancer Research (AECC), respectively., Mesenchymal stromal stem/cells (MSC) therapies are clinically used in a wide range of disorders based on their robust HLA-independent immunosuppressive and anti-inflammatory properties. However, the mechanisms underlying MSC therapeutic activity remain elusive as demonstrated by the unpredictable therapeutic efficacy of MSC infusions reported in multiple clinical trials. A seminal recent study showed that infused MSCs are actively induced to undergo apoptosis by recipient cytotoxic T cells, a mechanism that triggers in vivo recipient-induced immunomodulation by such apoptotic MSCs, and the need for such recipient cytotoxic cell activity could be replaced by the administration of ex vivo-generated apoptotic MSCs. Moreover, the use of MSC-derived extracellular vesicles (MSC-EVs) is being actively explored as a cellfree therapeutic alternative over the parental MSCs. We hypothesized that the introduction of a “suicide gene” switch into MSCs may offer on-demand in vivo apoptosis of transplanted MSCs. Here, we prompted to investigate the utility of the iCasp9/AP1903 suicide gene system in inducing apoptosis of MSCs. iCasp9/AP1903-induced apoptotic MSCs (MSCiCasp9+) were tested in vitro and in in vivo models of acute colitis. Our data show a very similar and robust immunosuppressive and anti-inflammatory properties of both “parental” alive MSCGFP+ cells and apoptotic MSCiCasp9+ cells in vitro and in vivo regardless of whether apoptosis was induced in vivo or in vitro before administering MSCiCasp9+ lysates. This development of an efficient iCasp9 switch may potentiate the safety of MSC-based therapies in the case of an adverse event and, will also circumvent current logistic technical limitations and biological uncertainties associated to MSC-EVs., Health Canada H4080-144541, Juan de la Cierva fellowship by the Spanish Ministry of Science and Innovation IJCI-2017-3317, Spanish Association of Cancer Research (AECC)
Published: 2022
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58. Distributed consensus-based secondary control of multi-terminal DC railway systems

Author: Olives Camps, Juan Carlos, Mauricio Ferramola, Juan Manuel, Maza Ortega, José María, Gómez Expósito, Antonio, Universidad de Sevilla. Departamento de Ingeniería Eléctrica, Universidad de Sevilla. TEP196: Sistemas de Energía Eléctrica, Spanish Ministry of Science and Innovation PID2021-124571OB-I00, Junta de Andalucía FLEX-REN P18-TP-3655, and Industrial and Technological Development Centre of Spain (under the research Project HySGrid+) CER-20191019
Subjects: Medium-voltage DC (MVDC), Secondary voltage control, Distributed cooperative control, Railway electrification, Multi-terminal DC (MTDC)
Abstract: Electrification of the transportation sector is one of the main pillars of the future decarbonized society. In this context, high-speed railway corridors are expected to reduce as much as possible their environmental impact while maintaining the safety and quality of service with minimum investments. In pursuing those objectives, the multi-terminal DC railway system may become the preferred paradigm. In contrast to conventional AC railway electrification schemes, the multi-terminal DC railway system requires the adoption of a control algorithm to establish suitable references for the power converters. This paper elaborates on a new consensus-based secondary control strategy, which can be implemented in a distributed fashion. In this approach, each power converter controller receives information from its nearest neighbors to adjust its operating point. A 600-km railway system, subject to realistic train traffic, is used to compare the performance of the proposed control strategy with that of purely local controllers. Numerical simulations evidence that the proposed distributed control scheme provides a compromise solution in terms of voltage drops and equalization of power converter loading, surpassing the performance of conventional state-of-the art controllers.
Published: 2023

59. Complexity and equivalency of multiset dimension and ID-colorings

Author: Hakanen, Anni, Yero, Ismael G., Laboratoire d'Informatique, de Modélisation et d'Optimisation des Systèmes (LIMOS), Ecole Nationale Supérieure des Mines de St Etienne (ENSM ST-ETIENNE)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), University of Turku, Universidad de Cádiz (UCA), Jenny and Antti Wihuri Foundation, Academy of Finland grant number 338797, Spanish Ministry of Science and Innovation grant PID2019-105824GB-I00, and ANR-21-CE48-0004,GRALMECO,Algorithmique des problèmes de couverture métriques dans les graphes(2021)
Subjects: FOS: Computer and information sciences, 05C12, 05C76, Discrete Mathematics (cs.DM), MSC 05C12, 05C76, ID-number, [INFO.INFO-DM]Computer Science [cs]/Discrete Mathematics [cs.DM], king grids, ID-colorings, multiset resolving set, [MATH.MATH-CO]Mathematics [math]/Combinatorics [math.CO], FOS: Mathematics, Mathematics - Combinatorics, Combinatorics (math.CO), multiset dimension, Computer Science - Discrete Mathematics
Abstract: This investigation is firstly focused into showing that two metric parameters represent the same object in graph theory. That is, we prove that the multiset resolving sets and the ID-colorings of graphs are the same thing. We also consider some computational and combinatorial problems of the multiset dimension, or equivalently, the ID-number of graphs. We prove that the decision problem concerning finding the multiset dimension of graphs is NP-complete. We consider the multiset dimension of king grids and prove that it is bounded above by $4$. We also give a characterization of the strong product graphs with one factor being a complete graph, and whose multiset dimension is not infinite.
Published: 2023

60. Residential greenspace and lung function decline over 20 years in a prospective cohort: The ECRHS study

Author: Markevych, Iana, Zhao, Tianyu, Fuertes, Elaine, Marcon, Alessandro, Dadvand, Payam, Vienneau, Danielle, Garcia Aymerich, Judith, Nowak, Dennis, de Hoogh, Kees, Jarvis, Deborah, Abramson, Michael J, Accordini, Simone, Amaral, Andre Fs, Bentouhami, Hayat, Jacobsen Bertelsen, Randi, Boudier, Anne, Bono, Roberto, Bowatte, Gayan, Casas, Lidia, Dharmage, Shyamali C, Forsberg, Bertil, Gislason, Thorarinn, Gnesi, Marco, Holm, Mathias, Jacquemin, Benedicte, Janson, Christer, Jogi, Rain, Johannessen, Ane, Keidel, Dirk, Leynaert, Benedicte, Maldonado Perez, José Antonio, Marchetti, Pierpaolo, Migliore, Enrica, Martínez-Moratalla, Jesús, Orru, Hans, Pin, Isabelle, Potts, James, Probst-Hensch, Nicole, Ranzi, Andrea, Sánchez-Ramos, José Luis, Siroux, Valerie, Soussan, David, Sunyer, Jordi, Urrutia Landa, Isabel, Villani, Simona, Heinrich, Joachim, Helmholtz Zentrum München (HMGU), Uniwersytet Jagielloński w Krakowie = Jagiellonian University (UJ), Ludwig-Maximilians-Universität München (LMU), German Center for Lung Research, Imperial College London, Università degli studi di Verona = University of Verona (UNIVR), Universitat Pompeu Fabra [Barcelona] (UPF), CIBER de Epidemiología y Salud Pública (CIBERESP), Instituto de Salud Global - Institute For Global Health [Barcelona] (ISGlobal), University of Basel (Unibas), Swiss Tropical and Public Health Institute [Basel], Monash University [Melbourne], University of Antwerp (UA), University of Bergen (UiB), Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), University of Melbourne, University of Peradeniya, Umeå University, University of Iceland [Reykjavik], University of Gothenburg (GU), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Uppsala University, Centre de recherche en épidémiologie et santé des populations (CESP), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, Complejo Hospitalario Universitario de Albacete, University of Tartu, CHU Grenoble, Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble, Population Health and Occupational Disease [Londres, Royaume-Uni], National Heart and Lung Institute [Londres, Royaume-Uni] (NHLI), Imperial College London-Imperial College London, LabEx Inflamex, University-Hospital Munich-Großhadern [München], and This study was specifically funded by the German Research Foundation (GZ: KA 4737/2–1 | NO 262/13–1 | HE 3294/12–1) and the Comprehensive Pneumology Center Munich (CPC-M), German Center for Lung Research (DZL), Munich, Germany.The authors appreciate the support provided by the National Natural Science Foundation of China (M-0420).The present analyses are part of the Ageing Lungs in European Cohorts (ALEC) Study (https://www.alecstudy.org), which has also received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 633212. The local investigators and funding agencies for the European Community Respiratory Health are reported in the Supplementary Material. ISGlobal is a member of CERCA Programme/Generalitat de Catalunya. These sources of funding had no role in the study design, the collection, analysis and interpretation of data, the writing of the report, and in the decision to submit the article for publication.Iana Markevych is supported from the 'NeuroSmog: Determining the impact of air pollution on the developing brain' (Nr. POIR.04.04.00–1763/18–00) which is implemented as part of the TEAM-NET programme of the Foundation for Polish Science, co-financed from EU resources, obtained from the European Regional Development Fund under the Smart Growth Operational Programme.Elaine Fuertes is support by the Imperial College Research Fellowship.We acknowledge support from the Spanish Ministry of Science and Innovation through the 'Centro de Excelencia Severo Ochoa 2019–2023' Program (CEX2018-000806-S), and support from the Generalitat de Catalunya through the CERCA Program to ISGlobal.
Subjects: Green space, spirometry, nature, ECRHS, FEV(1), FVC, Nature, FEV1, Spirometry, green space, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Human medicine, Green space Nature Spirometry FEV 1 FVC ECRHS
Abstract: Background: The few studies that have examined associations between greenspace and lung function in adulthood have yielded conflicting results and none have examined whether the rate of lung function decline is affected. Objective: We explored the association between residential greenspace and change in lung function over 20 years in 5559 adults from 22 centers in 11 countries participating in the population-based, international European Community Respiratory Health Survey. Methods: Forced expiratory volume in 1 s (FEV1) and forced vital capacity (FVC) were measured by spirometry when participants were approximately 35 (1990-1994), 44 (1999-2003), and 55 (2010-2014) years old. Greenness was assessed as the mean Normalized Difference Vegetation Index (NDVI) in 500 m, 300 m, and 100 m circular buffers around the residential addresses at the time of lung function measurement. Green spaces were defined as the presence of agricultural, natural, or urban green spaces in a circular 300 m buffer. Associations of these greenspace parameters with the rate of lung function change were assessed using adjusted linear mixed effects regression models with random intercepts for subjects nested within centers. Sensitivity analyses considered air pollution exposures. Results: A 0.2-increase (average interquartile range) in NDVI in the 500 m buffer was consistently associated with a faster decline in FVC (-1.25 mL/year [95% confidence interval: -2.18 to -0.33]). These associations were especially pronounced in females and those living in areas with low PM10 levels. We found no consistent associations with FEV1 and the FEV1/FVC ratio. Residing near forests or urban green spaces was associated with a faster decline in FEV1, while agricultural land and forests were related to a greater decline in FVC. Conclusions: More residential greenspace was not associated with better lung function in middle-aged European adults. Instead, we observed slight but consistent declines in lung function parameters. The potentially detrimental association requires verification in future studies.
Published: 2023
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61. Inborn errors of OAS–RNase L in SARS-CoV-2–related multisystem inflammatory syndrome in children

Author: Lee, Danyel, Le Pen, Jérémie, Yatim, Ahmad, Dong, Beihua, Aquino, Yann, Ogishi, Masato, Pescarmona, Rémi, Talouarn, Estelle, Rinchai, Darawan, Zhang, Peng, Perret, Magali, Liu, Zhiyong, Jordan, Iolanda, Elmas Bozdemir, Sefika, Bayhan, Gulsum Iclal, Beaufils, Camille, Bizien, Lucy, Bisiaux, Aurelie, Lei, Weite, Hasan, Milena, Chen, Jie, Gaughan, Christina, Asthana, Abhishek, Libri, Valentina, Luna, Joseph, Jaffré, Fabrice, Hoffmann, H.-Heinrich, Michailidis, Eleftherios, Moreews, Marion, Seeleuthner, Yoann, Bilguvar, Kaya, Mane, Shrikant, Flores, Carlos, Zhang, Yu, Arias, Andrés, Bailey, Rasheed, Schlüter, Agatha, Milisavljevic, Baptiste, Bigio, Benedetta, Le Voyer, Tom, Materna, Marie, Gervais, Adrian, Moncada-Velez, Marcela, Pala, Francesca, Lazarov, Tomi, Levy, Romain, Neehus, Anna-Lena, Rosain, Jérémie, Peel, Jessica, Chan, Yi-Hao, Morin, Marie-Paule, Pino-Ramirez, Rosa Maria, Belkaya, Serkan, Lorenzo, Lazaro, Anton, Jordi, Delafontaine, Selket, Toubiana, Julie, Bajolle, Fanny, Fumadó, Victoria, Dediego, Marta, Fidouh, Nadhira, Rozenberg, Flore, Pérez-Tur, Jordi, Chen, Shuibing, Evans, Todd, Geissmann, Frédéric, Lebon, Pierre, Weiss, Susan, Bonnet, Damien, Duval, Xavier, Pan-Hammarström, Qiang, Planas, Anna, Meyts, Isabelle, Haerynck, Filomeen, Pujol, Aurora, Sancho-Shimizu, Vanessa, Dalgard, Clifford, Bustamante, Jacinta, Puel, Anne, Boisson-Dupuis, Stéphanie, Boisson, Bertrand, Maniatis, Tom, Zhang, Qian, Bastard, Paul, Notarangelo, Luigi, Béziat, Vivien, Perez de Diego, Rebeca, Rodriguez-Gallego, Carlos, Su, Helen, Lifton, Richard, Jouanguy, Emmanuelle, Cobat, Aurélie, Alsina, Laia, Keles, Sevgi, Haddad, Elie, Abel, Laurent, Belot, Alexandre, Quintana-Murci, Lluis, Rice, Charles, Silverman, Robert, Zhang, Shen-Ying, Casanova, Jean-Laurent, Alavoine, Loubna, Behillil, Sylvie, Burdet, Charles, Charpentier, Charlotte, Dechanet, Aline, Descamps, Diane, Ecobichon, Jean-Luc, Enouf, Vincent, Frezouls, Wahiba, Houhou, Nadhira, Kafif, Ouifiya, Lehacaut, Jonathan, Letrou, Sophie, Lina, Bruno, Lucet, Jean-Christophe, Manchon, Pauline, Nouroudine, Mariama, Piquard, Valentine, Quintin, Caroline, Thy, Michael, Tubiana, Sarah, van der Werf, Sylvie, Vignali, Valérie, Visseaux, Benoit, Yazdanpanah, Yazdan, Chahine, Abir, Waucquier, Nawal, Migaud, Maria-Claire, Deplanque, Dominique, Djossou, Félix, Mergeay-Fabre, Mayka, Lucarelli, Aude, Demar, Magalie, Bruneau, Léa, Gérardin, Patrick, Maillot, Adrien, Payet, Christine, Laviolle, Bruno, Laine, Fabrice, Paris, Christophe, Desille-Dugast, Mireille, Fouchard, Julie, Malvy, Denis, Nguyen, Duc, Pistone, Thierry, Perreau, Pauline, Gissot, Valérie, Le Goas, Carole, Montagne, Samatha, Richard, Lucie, Chirouze, Catherine, Bouiller, Kévin, Desmarets, Maxime, Meunier, Alexandre, Lefèvre, Benjamin, Jeulin, Hélène, Legrand, Karine, Lomazzi, Sandra, Tardy, Bernard, Gagneux-Brunon, Amandine, Bertholon, Frédérique, Botelho-Nevers, Elisabeth, Christelle, Kouakam, Nicolas, Leturque, Roufai, Layidé, Amat, Karine, Couffin-Cadiergues, Sandrine, Espérou, Hélène, Hendou, Samia, Abolhassani, Hassan, Aguilera-Albesa, Sergio, Aiuti, Alessandro, Akcan, Ozge Metin, Akcay, Nihal, Alkan, Gulsum, Alkhater, Suzan, Allende, Luis Miguel, Alper, Yosunkaya, Amenzoui, Naima, Anderson, Mark, Arkin, Lisa, Aubart, Melodie, Avramenko, Iryna, Aydemir, Şehnaz, Gayretli Aydin, Zeynep Gökçe, Aytekin, Caner, Aytekin, Gökhan, Erol Aytekin, Selma, Bando, Silvia Yumi, Beland, Kathie, Biggs, Catherine, Bilbao Aburto, Agurtzane, Blanchard-Rohner, Geraldine, Blázquez-Gamero, Daniel, Bloomfield, Marketa, Bogunovic, Dusan, Bondarenko, Anastasia, Borghesi, Alessandro, Bousfiha, Amed Aziz, Boyarchuk, Oksana, Brodin, Petter, Bryceson, Yenan, Bucciol, Giorgia, Calcaterra, Valeria, Casari, Giorgio, Cavalcanti, Andre, Celik, Jale Bengi, Chrousos, George, Colobran, Roger, Condino-Neto, Antonio, Conti, Francesca, Cooper, Megan, Coskuner, Taner, Cyrus, Cyril, D’auria, Enza, Drolet, Beth, Bursal Duramaz, Burcu, El Zein, Loubna, Elnagdy, Marwa, Emiroglu, Melike, Erdeniz, Emine Hafize, Fabi, Marianna, Baris Feldman, Hagit, Fellay, Jacques, Fencl, Filip, Filippatos, Filippos, Freiss, Julie, Fremuth, Jiri, Gagro, Alenka, Garcia-Solis, Blanca, Vergine, Gianluca, González-Montelongo, Rafaela, Gul, Yahya, Gülhan, Belgin, Gultekin, Sara Sebnem Kilic, Gut, Marta, Halwani, Rabih, Hammarström, Lennart, Hatipoğlu, Nevin, Heath, James, Henrickson, Sarah, Hernandez-Brito, Elisa, Hoffman, Ilse, Hoste, Levi, Hsieh, Elena, Íñigo-Campos, Antonio, Itan, Yuval, Jabandziev, Petr, Kandemir, Bahar, Kanık-Yüksek, Saliha, Kapakli, Hasan, Karbuz, Adem, Kasapcopur, Ozgur, Kechiche, Robin, Kendir Demirkol, Yasemin, Kilic, Omer, Hansen, Stella Kim, Klocperk, Adam, Lau, Yu-Lung, Lebl, Jan, Lorenzo-Salazar, José, Lucas, Carrie, Maglorius, Majistor, Marque, Laura, Novoa Medina, Yeray, Montesdeoca Melián, Abián, Mentis, Alexios-Fotios, Pato, Michele, Michos, Athanasios, Milner, Joshua, Mogensen, Trine, Muñoz-Barrera, Adrián, Nepesov, Serdar, Farela Neves, João, Ng, Ashley, Ng, Lisa, Novelli, Antonio, Novelli, Giuseppe, Oz, Fatma Nur, Ocejo-Viñals, J. Gonzalo, Okada, Satoshi, Orbak, Zerrin, Kilic, Ahmet Osman, Ouair, Hind, Öz, Şadiye Kübra Tüter, Özçelik, Tayfun, Özkan, Esra Akyüz, Parlakay, Aslınur Özkaya, Pato, Carlos, Paz-Artal, Estela, Pelham, Simon, Pellier, Isabelle, Philippot, Quentin, Planas-Serra, Laura, Plassart, Samira, Pokorna, Petra, Polat, Meltem, Poli, Cecilia, Prando, Carolina, Renia, Laurent, Rivière, Jacques, Rodríguez-Palmero, Agustí, Roussel, Lucie, Rubio-Rodriguez, Luis, Salifu, Moro, Sasek, Lumir, Sasia, Laura, Scherbina, Anna, Schmitt, Erica, Sediva, Anna, Sevketoglu, Esra, Slaba, Katerina, Slaby, Ondrej, Sobh, Ali, Solé-Violán, Jordi, Soler-Palacin, Pere, de Somer, Lien, Sözeri, Betül, Spaan, András, Stepanovskiy, Yuriy, Tangye, Stuart, Tanir, Gonul, Tatsi, Elizabeth Barbara, Thorball, Christian, Hancerli Torun, Selda, Turvey, Stuart, Uddin, Mohammed, Uyar, Emel, Valencia-Ramos, Juan, van den Rym, Ana Maria, Vatansev, Hulya, Castillo de Vera, Martín, Vermeulen, François, Vinh, Donald, Volokha, Alla, von Bernuth, Horst, Wouters, Carine, Yahşi, Aysun, Yarar, Volkan, Yesilbas, Osman, Yıldız, Mehmet, Zatz, Mayana, Zawadzki, Pawel, Zuccotti, Gianvincenzo, Rockefeller University [New York], Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Institut de génétique humaine (IGH), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Génomique évolutive, modélisation et santé (GEMS), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de référence des rhumatismes inflammatoires et maladies auto-immunes systémiques rares de l’enfant / National Referee Centre for Rheumatic and AutoImmune and Systemic Diseases in Children [Lyon] (RAISE), Hospices Civils de Lyon (HCL), Centre Hospitalier Lyon Sud [CHU - HCL] (CHLS), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-École nationale supérieure agronomique de Toulouse (ENSAT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Sidra Medicine [Doha, Qatar], BIOASTER Technology Research Institute, Lyon, France, St. Giles Laboratory of Human Genetics of Infectious Diseases, Department of Paediatrics and Intensive Care, Hospital Universitari Sant Joan de Deu, Human genetics of infectious diseases : Mendelian predisposition (Equipe Inserm U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Département de Pédiatrie et maladies infectieuses [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Génétique Evolutive Humaine - Human Evolutionary Genetics, Centre de Recherche Translationnelle - Center for Translational Science (CRT), Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Shanghai Jiaotong University, Sheffield Hallam University, Institut Jean Lamour (IJL), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Infection, Anti-microbiens, Modélisation, Evolution (IAME (UMR_S_1137 / U1137)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Université Sorbonne Paris Nord, Centre d'investigation Clinique [CHU Bichat] - Épidémiologie clinique (CIC 1425), AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Biodiversité et Epidémiologie des Bactéries pathogènes - Biodiversity and Epidemiology of Bacterial Pathogens, Centre d'Investigation Clinique - Innovation Technologique de Lille - CIC 1403 - CIC 9301 (CIC Lille), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Centre d'Investigation Clinique Antilles-Guyane (CIC - Antilles Guyane), Université des Antilles et de la Guyane (UAG)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pointe-à-Pitre/Abymes [Guadeloupe] -CHU de Fort de France-Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française], Centre d'Investigation Clinique de La Réunion - INSERM (CIC 1410), Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Universitaire de La Réunion (CHU La Réunion), Centre d'Investigation Clinique [Rennes] (CIC), Université de Rennes (UR)-Hôpital Pontchaillou-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Pontchaillou [Rennes], The Laboratory of Human Genetics of Infectious Diseases is supported by the Howard Hughes Medical Institute, the Rockefeller University, the St. Giles Foundation, the National Institutes of Health (NIH) (R01AI088364 and R21AI160576), the National Center for Advancing Translational Sciences (NCATS), NIH Clinical and Translational Science Award (CTSA) program (UL1TR001866), the Yale Center for Mendelian Genomics and the GSP Coordinating Center funded by the National Human Genome Research Institute (NHGRI) (UM1HG006504 and U24HG008956), the Yale High-Performance Computing Center (S10OD018521), the Fisher Center for Alzheimer’s Research Foundation, the Meyer Foundation, the JBP Foundation, the French National Research Agency (ANR) under the 'Investments for the Future' program (ANR-10-IAHU-01), the Integrative Biology of Emerging Infectious Diseases Laboratory of Excellence (ANR-10-LABX-62-IBEID), the French Foundation for Medical Research (FRM) (EQU201903007798), the ANR GenMISC (ANR-21-COVR-039), the ANRS-COV05, ANR GENVIR (ANR-20-CE93-003) and ANR AABIFNCOV (ANR-20-CO11-0001) projects, the ANR-RHU program (ANR-21-RHUS-08), the European Union’s Horizon 2020 research and innovation program under grant agreement 824110 (EASI-genomics), the HORIZON-HLTH-2021-DISEASE-04 program under grant agreement 01057100 (UNDINE), the ANR-RHU Program ANR-21-RHUS-08 (COVIFERON), the Square Foundation, Grandir – Fonds de solidarité pour l’enfance, the Fondation du Souffle, the SCOR Corporate Foundation for Science, the French Ministry of Higher Education, Research, and Innovation (MESRI-COVID-19), Institut National de la Santé et de la Recherche Médicale (INSERM), and Paris Cité University. We acknowledge support from the National Institute of Allergy and Infectious Diseases (NIAID) of the NIH under award R01AI104887 to R.H.S. and S.R.W. The Laboratory of Human Evolutionary Genetics (Institut Pasteur) is supported by the Institut Pasteur, the Collège de France, the French Government’s Investissement d’Avenir program, Laboratoires d’Excellence 'Integrative Biology of Emerging Infectious Diseases' (ANR-10-LABX-62-IBEID) and 'Milieu Intérieur' (ANR-10-LABX-69-01), the Fondation de France (no. 00106080), the FRM (Equipe FRM DEQ20180339214 team), and the ANR COVID-19-POPCELL (ANR-21-CO14-0003-01). A.Puj. is supported by ACCI20-759 CIBERER, EasiGenomics H2020 Marató TV3 COVID 2021-31-33, the HORIZON-HLTH-2021-ID: 101057100 (UNDINE), the Horizon 2020 program under grant no. 824110 (EasiGenomics grant no. COVID-19/PID12342), and the CERCA Program/Generalitat de Catalunya. The Canarian Health System sequencing hub was funded by the Instituto de Salud Carlos III (COV20_01333 and COV20_01334), the Spanish Ministry of Science and Innovation (RTC-2017-6471-1, AEI/FEDER, UE), Fundación MAPFRE Guanarteme (OA21/131), and Cabildo Insular de Tenerife (CGIEU0000219140 and 'Apuestas científicas del ITER para colaborar en la lucha contra la COVID-19'). The CoV-Contact Cohort was funded by the French Ministry of Health and the European Commission (RECOVER project). Our studies are also funded by the Ministry of Health of the Czech Republic Conceptual Development of Research Organization (FNBr, 65269705) and ANID COVID0999 funding in Chile. G. Novelli and A. Novelli are supported by Regione Lazio (Research Group Projects 2020) No. A0375-2020-36663, GecoBiomark. A.M.P., M.L.D., and J.P.-T. are supported by the Inmungen-CoV2 project of CSIC. This work was supported in part by the Intramural Research Program of the NIAID, NIH. The research work of A.M.P, M.L.D., and J.P.-T. was funded by the European Commission –NextGenerationEU (Regulation EU 2020/2094), through CSIC’s Global Health Platform (PTI Salud Global). I.M. is a senior clinical investigator at FWO Vlaanderen supported by a VIB GC PID grant, by FWO grants G0B5120N (DADA2) and G0E8420N, and by the Jeffrey Modell Foundation. I.M. holds an ERC-StG MORE2ADA2 grant and is also supported by ERN-RITA. A.Y. is supported by fellowships from the European Academy of Dermatology and Venereology and the Swiss National Science Foundation and by an Early Career Award from the Thrasher Research Fund. Y.-H.C. is supported by an A*STAR International Fellowship (AIF). M.O. was supported by the David Rockefeller Graduate Program, the New York Hideyo Noguchi Memorial Society (HNMS), the Funai Foundation for Information Technology (FFIT), the Honjo International Scholarship Foundation (HISF), and the National Cancer Institute (NCI) F99 Award (F99CA274708). A.A.A. was supported by Ministerio de Ciencia Tecnología e Innovación MINCIENCIAS, Colombia (111584467551/CT 415-2020). D.L. is supported by a fellowship from the FRM for medical residents and fellows. E.H. received funding from the Bank of Montreal Chair of Pediatric Immunology, Foundation of CHU Sainte-Justine, CIHR grants PCC-466901 and MM1-181123, and a Canadian Pediatric Society IMPACT study. Q.P.-H. received funding from the European Union’s Horizon 2020 research and innovation program (ATAC, 101003650), the Swedish Research Council, and the Knut and Alice Wallenberg Foundation. Work in the Laboratory of Virology and Infectious Disease was supported by NIH grants P01AI138398-S1, 2U19AI111825, R01AI091707-10S1, and R01AI161444, a George Mason University Fast Grant, the G. Harold and Leila Y. Mathers Charitable Foundation, the Meyer Foundation, and the Bawd Foundation. R.P.L. is on the board of directors of both Roche and the Roche subsidiary Genentech. J.L.P. was supported by a Francois Wallace Monahan Postdoctoral Fellowship at the Rockefeller University and by a European Molecular Biology Organization Long-Term Fellowship (ALTF 380-2018)., ANR-10-IAHU-0001,Imagine,Institut Hospitalo-Universitaire Imagine(2010), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), ANR-21-COVR-0039,GenMIS-C,Recherche des Déficits immunitaires innées monogéniques prédisposant au syndrome inflammatoire multisystémique chez l'enfant.(2021), ANR-20-CE93-0003,GENVIR,Analyse multi-omique de l'immunité anti-virale: de l'identification des circuits biologiques pertinents à la découverte de défauts monogéniques héréditaires de l'immunité chez les patients avec infections virales sévères(2020), ANR-20-CO11-0001,AABIFNCOV,Bases génétiques et immunologiques des auto-anticorps contre les interférons de type I prédisposant aux formes sévères de COVID-19.(2020), ANR-21-RHUS-0008,COVIFERON,Covid-19 and interferons: from discovery to therapy(2021), ANR-10-LABX-0069,MILIEU INTERIEUR,GENETIC & ENVIRONMENTAL CONTROL OF IMMUNE PHENOTYPE VARIANCE: ESTABLISHING A PATH TOWARDS PERSONALIZED MEDICINE(2010), ANR-21-CO14-0003,COVID-19-POPCELL,Facteurs génétiques et infectieux à l'origine de la variabilité populationnelle de la réponse immunitaire à l'infection par le SARS-CoV-2(2021), European Project: 824110,H2020-INFRAIA-2018-1,EASI-Genomics(2019), European Project: 101057100,UNDINE, Howard Hughes Medical Institute, Rockefeller University, St. Giles Foundation, National Institutes of Health (US), Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación (España), Fundación Mapfre, Cabildo de Tenerife, Fundació La Marató de TV3, Centro de Investigación Biomédica en Red Enfermedades Raras (España), Consejo Superior de Investigaciones Científicas (España), European Commission, and Pérez-Tur, Jordi
Subjects: Multidisciplinary, Settore MED/03, [SDV]Life Sciences [q-bio], Medicine and Health Sciences, CoV-Contact Cohort§
Abstract: 62 páginas, 5 figuras, 2 tablas, Multisystem inflammatory syndrome in children (MIS-C) is a rare and severe condition that follows benign COVID-19. We report autosomal recessive deficiencies of OAS1, OAS2, or RNASEL in five unrelated children with MIS-C. The cytosolic double-stranded RNA (dsRNA)-sensing OAS1 and OAS2 generate 2'-5'-linked oligoadenylates (2-5A) that activate the single-stranded RNA-degrading ribonuclease L (RNase L). Monocytic cell lines and primary myeloid cells with OAS1, OAS2, or RNase L deficiencies produce excessive amounts of inflammatory cytokines upon dsRNA or severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) stimulation. Exogenous 2-5A suppresses cytokine production in OAS1-deficient but not RNase L-deficient cells. Cytokine production in RNase L-deficient cells is impaired by MDA5 or RIG-I deficiency and abolished by mitochondrial antiviral-signaling protein (MAVS) deficiency. Recessive OAS-RNase L deficiencies in these patients unleash the production of SARS-CoV-2-triggered, MAVS-mediated inflammatory cytokines by mononuclear phagocytes, thereby underlying MIS-C, The Laboratory of Human Genetics of Infectious Diseases is supported by the Howard Hughes Medical Institute, the Rockefeller University, the St. Giles Foundation, the National Institutes of Health (NIH) (R01AI088364 and R21AI160576), the National Center for Advancing Translational Sciences (NCATS), NIH Clinical and Translational Science Award (CTSA) program (UL1TR001866), the Yale Center for Mendelian Genomics and the GSP Coordinating Center funded by the National Human Genome Research Institute (NHGRI) (UM1HG006504 and U24HG008956), the Yale High-Performance Computing Center (S10OD018521), the Fisher Center for Alzheimer’s Research Foundation, the Meyer Foundation, the JBP Foundation, the French National Research Agency (ANR) under the “Investments for the Future” program (ANR-10-IAHU-01), the Integrative Biology of Emerging Infectious Diseases Laboratory of Excellence (ANR-10- LABX-62-IBEID), the French Foundation for Medical Research (FRM) (EQU201903007798), the ANR GenMISC (ANR-21-COVR-039), the ANRS-COV05, ANR GENVIR (ANR-20-CE93-003) and ANR AABIFNCOV (ANR-20-CO11-0001) projects, the ANR-RHU program (ANR-21-RHUS-08), the European Union’s Horizon 2020 research and innovation program under grant agreement 824110 (EASI-genomics), the HORIZON-HLTH-2021-DISEASE-04 program under grant agreement 01057100 (UNDINE), the ANR-RHU Program ANR-21- RHUS-08 (COVIFERON), the Square Foundation, Grandir – Fonds de solidarité pour l’enfance, the Fondation du Souffle, the SCOR Corporate Foundation for Science, the French Ministry of Higher Education, Research, and Innovation (MESRI-COVID-19), Institut National de la Santé et de la Recherche Médicale (INSERM), and Paris Cité University. We acknowledge support from the National Institute of Allergy and Infectious Diseases (NIAID) of the NIH under award R01AI104887 to R.H.S. and S.R.W. The Laboratory of Human Evolutionary Genetics (Institut Pasteur) is supported by the Institut Pasteur, the Collège de France, the French Government’s Investissement d’Avenir program, Laboratoires d’Excellence “Integrative Biology of Emerging Infectious Diseases” (ANR-10-LABX-62-IBEID) and “Milieu Intérieur” (ANR-10-LABX-69-01), the Fondation de France (no. 00106080), the FRM (Equipe FRM DEQ20180339214 team), and the ANR COVID-19-POPCELL (ANR-21-CO14-0003-01). A.Puj. is supported by ACCI20-759 CIBERER, EasiGenomics H2020 Marató TV3 COVID 2021-31-33, the HORIZON-HLTH-2021-ID: 101057100 (UNDINE), the Horizon 2020 program under grant no. 824110 (EasiGenomics grant no. COVID-19/PID12342), and the CERCA Program/Generalitat de Catalunya. The Canarian Health System sequencing hub was funded by the Instituto de Salud Carlos III (COV20_01333 and COV20_01334), the Spanish Ministry of Science and Innovation (RTC-2017-6471-1; AEI/FEDER, UE), Fundación MAPFRE Guanarteme (OA21/131), and Cabildo Insular de Tenerife (CGIEU0000219140 and “Apuestas científicas del ITER para colaborar en la lucha contra la COVID-19”). The CoV-Contact Cohort was funded by the French Ministry of Health and the European Commission (RECOVER project). Our studies are also funded by the Ministry of Health of the Czech Republic Conceptual Development of Research Organization (FNBr, 65269705) and ANID COVID0999 funding in Chile. G. Novelli and A. Novelli are supported by Regione Lazio (Research Group Projects 2020) No. A0375-2020-36663, GecoBiomark. A.M.P., M.L.D., and J.P.-T. are supported by the Inmungen-CoV2 project of CSIC. This work was supported in part by the Intramural Research Program of the NIAID, NIH. The research work of A.M..P, M.L.D., and J.P.-T. was funded by the European Commission –NextGenerationEU (Regulation EU 2020/2094), through CSIC’s Global Health Platform (PTI Salud Global). I.M. is a senior clinical investigator at FWO Vlaanderen supported by a VIB GC PID grant, by FWO grants G0B5120N (DADA2) and G0E8420N, and by the Jeffrey Modell Foundation. I.M. holds an ERC-StG MORE2ADA2 grant and is also supported by ERN-RITA. A.Y. is supported by fellowships from the European Academy of Dermatology and Venereology and the Swiss National Science Foundation and by an Early Career Award from the Thrasher Research Fund. Y.-H.C. is supported by an A*STAR International Fellowship (AIF). M.O. was supported by the David Rockefeller Graduate Program, the New York Hideyo Noguchi Memorial Society (HNMS), the Funai Foundation for Information Technology (FFIT), the Honjo International Scholarship Foundation (HISF), and the National Cancer Institute (NCI) F99 Award (F99CA274708). A.A.A. was supported by Ministerio de Ciencia Tecnología e Innovación MINCIENCIAS, Colombia (111584467551/CT 415-2020). D.L. is supported by a fellowship from the FRM for medical residents and fellows. E.H. received funding from the Bank of Montreal Chair of Pediatric Immunology, Foundation of CHU Sainte-Justine, CIHR grants PCC-466901 and MM1-181123, and a Canadian Pediatric Society IMPACT study. Q.P.-H. received funding from the European Union’s Horizon 2020 research and innovation program (ATAC, 101003650), the Swedish Research Council, and the Knut and Alice Wallenberg Foundation. Work in the Laboratory of Virology and Infectious Disease was supported by NIH grants P01AI138398-S1, 2U19AI111825, R01AI091707-10S1, and R01AI161444; a George Mason University Fast Grant; the G. Harold and Leila Y. Mathers Charitable Foundation; the Meyer Foundation; and the Bawd Foundation. R.P.L. is on the board of directors of both Roche and the Roche subsidiary Genentech. J.L.P. was supported by a Francois Wallace Monahan Postdoctoral Fellowship at the Rockefeller University and by a European Molecular Biology Organization Long-Term Fellowship (ALTF 380-2018).
Published: 2023
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62. Influence of a temporary restriction of dietary protein in prepubertal ewe lambs on first lactation milk traits and response to a mammary gland inflammatory challenge

Author: Rocío Pelayo, Héctor Marina, Aroa Suárez-Vega, Gonzalo Hervás, Cristina Esteban-Blanco, Blandine Gausseres, Gilles Foucras, Juan J. Arranz, Beatriz Gutiérrez-Gil, European Commission, Ministerio de Ciencia e Innovación (España), Hervás, Gonzalo, Producción Animal, Facultad de Veterinaria, Universidad de León [León], Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), The nutritional and inflammatory challenge experiments and the sample collection described in this study were funded by the SMARTER project through the Horizon 2020 research and innovation program of the European Commission (Grant Agreement No. 772787). The cytokine/interleukine panel analyses were funded by the Spanish national project EPIMILKSHEEP (Ref. RTI2018–093535-B-I00), funded by the Spanish Ministry of Science and Innovation., European Project: 772787,H2020-EU.3.2.1.1., and H2020-EU.3.2.,SMARTER(2018)
Subjects: [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, General Veterinary, Producción animal, Dairy sheep, Mammary gland, Inflammation response, Nutritional challenge, 3104 Producción Animal, Responsible Consumption and Production
Abstract: 9 páginas, 3 figuras, 2 tablas., This study evaluated the influence of a temporary nutritional protein restriction (NPR) performed, under commercial conditions, in prepubertal female lambs on first lactation milk production traits and the inflammatory response triggered by an inflammatory challenge of the. From 40 Assaf female lambs, we defined a control group (C n = 20), which received a standard diet for replacement lambs and the NPR group (n = 20), which received the same diet but without soybean meal between 3 and 5 months of age. About 150 days after lambing, 24 of these ewes (13 NPR, 11C) were subjected to an intramammary infusion of E. coli lipopolysaccharide (LPS). Our dynamic study identified indicator traits of local (SCC) and systemic (rectal Ta, IL-6, CXCL8, IL-10, IL-36RA, VEGF-A) response to the LPS challenge. The NPR did not show significant effects on milk production traits and did not affect the SCC and rectal Ta after the LPS challenge. However, the NPR had a significant influence on 8 of the 14 plasma biomarkers analysed, in all the cases with higher relative values in the C group. The effects observed on VEGF-A (involved in vasculogenesis during mammary gland development and vascular permeability) and IL-10 (a regulatory cytokine classically known by its anti-inflammatory action) are the most remarkable to explain the differences found between groups. Whereas further studies should be undertaken to confirm these results, our findings are of interest considering the current concern about the future world's demand for protein and the need for animal production systems to evolve toward sustainability., The nutritional and inflammatory challenge experiments and the sample collection described in this study were funded by the SMARTER project through the Horizon 2020 research and innovation program of the European Commission (Grant Agreement No. 772787). The cytokine/interleukine panel analyses were funded by the Spanish national project EPIMILKSHEEP (Ref. RTI2018–093535-B-I00), funded by the Spanish Ministry of Science and Innovation.
Published: 2023
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63. Oceanic resurge deposits at the Rochechouart impact structure (France) suggest a marine target environment

Author: Jens Ormö, Erik Sturkell, Philippe Lambert, Sylvie Bourquin, Jean-Baptiste Cherfils, University of Gothenburg (GU), Centre International de Recherches et de Restitution sur les Impacts et sur Rochechouart (CIRIR), Géosciences Rennes (GR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), Spanish Ministry of Science and Innovation/State Agency of Research (ESP2017-87676-C5-1-R et PID2021-125883NB-C22), Spanish GovernmentMDM-2017-0737, MCIN/AEI, and 'ERDF A way of making Europe'
Subjects: [SDU.STU.ST]Sciences of the Universe [physics]/Earth Sciences/Stratigraphy, Geology
Abstract: The Rochechouart impact structure, located in the western part of the Massif Central in France, has been suggested to be one of the largest impact structures in western Europe. Various age datings have placed the event in a span from the Late Triassic to the Early Jurassic, but the most recent works favour a Late Triassic age. Very little is known about the target environment at the time and location of the impact event. Seemingly coeval, potential tsunamites along palaeoshorelines of the sea that covered parts of continental Europe at the time have been suggested to be related to the impact event and may indicate a marine target setting. Here we apply the method of visual line-logging of the graded suevite in the Chassenon SC2 drill core. This method has previously been used to investigate the depositional environment of similar deposits in several marine target impact craters. It allowed us to compare the deposits at these craters with those at Rochechouart, and in this way not only confirm the marine target setting, but also estimate the target water depth to be ∼200 m. Altogether, our results indicate a palaeogeographic target setting in a newly opened seaway connecting the Paris Basin with the Aquitaine Basin, which may indicate an age of impact at the younger end of the hitherto suggested age-span, i.e. in the late Rhaetian – Early Jurassic.
Published: 2023
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64. Metagenomic 16S rDNA Illumina tags are a powerful alternative to amplicon sequencing to explore diversity and structure of microbial communities

Author: Logares, Ramiro, Sunagawa, Shinichi, Salazar, Guillem, Cornejo-Castillo, Francisco M., Ferrera, Isabel, Sarmento, Hugo, Hingamp, Pascal, Ogata, Hiroyuki, de Vargas, Colomban, Lima-Mendez, Gipsi, Raes, Jeroen, Poulain, Julie, Jaillon, Olivier, Wincker, Patrick, Kandels-Lewis, Stefanie, Karsenti, Eric, Bork, Peer, Acinas, Silvia G., Institute of Marine Sciences / Institut de Ciències del Mar [Barcelona] (ICM), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), European Molecular Biology Laboratory, Instituto de Fisica [Mexico] (UASLP), Universidad Autonoma de San Luis Potosi [México] (UASLP), Information génomique et structurale (IGS), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Education academy of computational life sciences, Tokyo Institute of Technology [Tokyo] (TITECH), Evolution des Protistes et Ecosystèmes Pélagiques (EPEP), Adaptation et diversité en milieu marin (AD2M), Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Department of Bioscience Engineering, Vrije Universiteit Brussel (VUB), Rega Institute - Department of Microbiology and Immunology, Université Catholique de Louvain = Catholic University of Louvain (UCL), VIB Center for the Biology of Disease, Flanders Institute for Biotechnology, Institut de Génomique d'Evry (IG), Université Paris-Saclay-Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Génomique métabolique (UMR 8030), Genoscope - Centre national de séquençage [Evry] (GENOSCOPE), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), European Molecular Biology Laboratory [Heidelberg] (EMBL), Max Delbrück Center for Molecular Medicine [Berlin] (MDC), Helmholtz-Gemeinschaft = Helmholtz Association, CNRD, EMBL, Biosphere 2, agnes b., Veolia Environmental Foundation, Region Bretagne, World Courier, Cap L'Orient, Foundation EDF Diversiterre, FRB, Prince Albert II de Monaco Foundation, Etienne Bourgois, Genoscope/CEA, Tara Foundations teams and crew, Spanish Ministry of Science and Innovation, FP7-OCEAN-2011 `Micro B3', Agencia de Gestio d'Ajusts Universitaris i Reserca [CTM2010-12317-E, CONES 2010-0036], MicroOcean PANGENOMICS [CGL2011-26848/BOS], Spanish Ministry of Science and Innovation (MICINN), Marie Curie Intra European Fellowship (MASTDIEV) [PIEF-GA-2009-235365], Marie Curie Intra European Fellowship (EU), Spanish Ministry of Science and Innovation [JCI-2010-06594], PhD JAE-Predoc (CSIC), UPMC, FPI (MICINN), Agence Nationale de la Recherche, ANR [ANR-09-GENM-031, ANR-09-BLAN-0348, ANR-11-BTBR-0008, Tara-GirusANR-09-PCS-GENM-218], Fund for Scientific Research Flanders (FWO), [BCV-2010-3-0003], [2011-2-0003/3-0005], VIB, Stazione Zoologica Anton Dohrn, UNIMIB, ANR, FWO, BIO5, Institut de Biologie François JACOB (JACOB), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université d'Évry-Val-d'Essonne (UEVE)
Subjects: [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences
Abstract: International audience; Sequencing of 16S rDNA polymerase chain reaction (PCR) amplicons is the most common approach for investigating environmental prokaryotic diversity, despite the known biases introduced during PCR. Here we show that 16S rDNA fragments derived from Illumina-sequenced environmental metagenomes (mitags) are a powerful alternative to 16S rDNA amplicons for investigating the taxonomic diversity and structure of prokaryotic communities. As part of the TaraOceans global expedition, marine plankton was sampled in three locations, resulting in 29 subsamples for which metagenomes were produced by shotgun Illumina sequencing (ca. 700Gb). For comparative analyses, a subset of samples was also selected for Roche-454 sequencing using both shotgun (m454tags; 13 metagenomes, ca. 2.4Gb) and 16S rDNA amplicon (454tags; ca. 0.075Gb) approaches. Our results indicate that by overcoming PCR biases related to amplification and primer mismatch, mitags may provide more realistic estimates of community richness and evenness than amplicon 454tags. In addition, mitags can capture expected beta diversity patterns. Using mitags is now economically feasible given the dramatic reduction in high-throughput sequencing costs, having the advantage of retrieving simultaneously both taxonomic (Bacteria, Archaea and Eukarya) and functional information from the same microbial community.
Published: 2014
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65. CB1 blockade potentiates down-regulation of lipogenic gene expression in perirenal adipose tissue in high carbohydrate diet-induced obesity

Author: Ana Luisa Gavito, Fernando Rodríguez de Fonseca, Sergio Arrabal, Patricia Rivera, Dolores Bautista, Ana Martínez, Miguel Romero-Cuevas, Juan Suárez, Francisco Javier Pavón, Antonia Serrano, Margarita Vida, Elena Baixeras, [Vida,M, Rivera,P, Gavito,AL, Suárez,J, Pavón,FJ, Arrabal,S, Romero-Cuevas,M, Rodriguez de Fonseca,F, Serrano.A] Unidad de Gestión Clínica de Salud Mental, Hospital Regional Universitario de Málaga, Universidad de Málaga, Málaga, Spain. [Baixeras,E] Unidad de Gestión Clínica de Medicina Interna, Hospital Regional Universitario de Málaga, Universidad de Málaga, Málaga, Spain. [Bautista,D] Unidad de Gestión Clínica de Anatomía Patológica, Hospital Regional Universitario de Málaga, Universidad de Málaga, Málaga, Spain. [Vida,M, Bautista,D, Rodríguez de Fonseca,F, Serrano,A, Baixeras,E] Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario de Málaga, Universidad de Málaga, Málaga, Spain. [Martínez,A] Instituto de Química Médica Lora Tamayo, Consejo Superior de Investigaciones Científicas. Madrid, Spain. [Vida,M, RiveraP, Rodríguez de Fonseca, Serrano,A] CIBER de Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Santiago de Compostela, Spain., This work was supported by Grants: from the Spanish Institute of Health ‘‘Carlos III (PI061109, CP12/03109, CIBERobn EU-ERDF-CB06/03/1008), from the Andalusian Ministry of Health (PI0552), from Andalusian Ministry of Economy, Innovation, Science and Employment EU-ERDF (CTS-8221 and CTS-433), from Spanish Ministry of Science and Innovation (SAF2010-20521), and from European Union’s 7th Framework Programme (Health- F2-2008-223713, REPROBESITY). I3SNS Program of the Spanish National Health System of Spanish Ministry of Science and Innovation.
Subjects: Male, Time Factors, Anatomy::Urogenital System::Urinary Tract::Kidney [Medical Subject Headings], Technology, Industry, Agriculture::Food and Beverages::Food::Dietary Carbohydrates [Medical Subject Headings], Phenomena and Processes::Physical Phenomena::Time::Time Factors [Medical Subject Headings], Obesidad, Adipose tissue, lcsh:Medicine, Riñón, Phenomena and Processes::Physiological Phenomena::Body Constitution::Body Weights and Measures::Body Size::Body Weight [Medical Subject Headings], Signal transduction, ERK signaling cascade, Kidney, Phenomena and Processes::Physiological Phenomena::Body Constitution::Body Weights and Measures::Body Size::Body Weight::Overweight::Obesity [Medical Subject Headings], Biochemistry, Phenomena and Processes::Physiological Phenomena::Physiological Processes::Pharmacological Processes::Down-Regulation [Medical Subject Headings], Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Receptors, Cell Surface::Receptors, G-Protein-Coupled::Receptors, Cannabinoid::Receptor, Cannabinoid, CB1 [Medical Subject Headings], Molecular cell biology, 0302 clinical medicine, Piperidines, Receptor, Cannabinoid, CB1, Gene expression, Organisms::Eukaryota::Animals [Medical Subject Headings], Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Rats::Rats, Wistar [Medical Subject Headings], lcsh:Science, 0303 health sciences, Multidisciplinary, Signaling cascades, food and beverages, Neurochemistry, Animal Models, Lipids, PKA signaling cascade, 3. Good health, Lipogenesis, Carbohydrate Metabolism, lipids (amino acids, peptides, and proteins), Neurochemicals, Research Article, medicine.medical_specialty, Adipose Tissue, White, Phenomena and Processes::Metabolic Phenomena::Metabolism::Lipid Metabolism::Lipogenesis [Medical Subject Headings], Tejido adiposo blanco, Check Tags::Male [Medical Subject Headings], Down-Regulation, 030209 endocrinology & metabolism, Biology, Diet, High-Fat, Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Transcription Factors [Medical Subject Headings], Alimentación rica en grasa, 03 medical and health sciences, Phenomena and Processes::Physiological Phenomena::Nutritional Physiological Phenomena::Diet::Diet, High-Fat [Medical Subject Headings], Model Organisms, Downregulation and upregulation, Internal medicine, Dietary Carbohydrates, medicine, Animals, Lipogénesis, Obesity, Rats, Wistar, Liver X receptor, Carbohydrate-responsive element-binding protein, Transcription factor, 030304 developmental biology, Body Weight, lcsh:R, Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Signal Transduction [Medical Subject Headings], Peso corporal, Chemicals and Drugs::Heterocyclic Compounds::Heterocyclic Compounds, 1-Ring::Piperidines [Medical Subject Headings], Lipid Metabolism, Rats, Sterol regulatory element-binding protein, Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Rats [Medical Subject Headings], Anatomy::Tissues::Connective Tissue::Adipose Tissue::Adipose Tissue, White [Medical Subject Headings], Metabolism, Endocrinology, Chemicals and Drugs::Heterocyclic Compounds::Heterocyclic Compounds, 1-Ring::Azoles::Pyrazoles [Medical Subject Headings], Carbohidratos dietéticos, Pyrazoles, Rat, lcsh:Q, Transcription Factors, Neuroscience, Endocannabinoids
Abstract: De novo lipogenesis and hypercaloric diets are thought to contribute to increased fat mass, particularly in abdominal fat depots. CB1 is highly expressed in adipose tissue, and CB1-mediated signalling is associated with stimulation of lipogenesis and diet-induced obesity, though its contribution to increasing fat deposition in adipose tissue is controversial. Lipogenesis is regulated by transcription factors such as liver X receptor (LXR), sterol-response element binding protein (SREBP) and carbohydrate- responsive-element-binding protein (ChREBP). We evaluated the role of CB 1 in the gene expression of these factors and their target genes in relation to lipogenesis in the perirenal adipose tissue (PrAT) of rats fed a high-carbohydrate diet (HCHD) or a high-fat diet (HFD). Both obesity models showed an up-regulated gene expression of CB1 and Lxrα in this adipose pad. The Srebf-1 and ChREBP gene expressions were down-regulated in HFD but not in HCHD. The expression of their target genes encoding for lipogenic enzymes showed a decrease in diet-induced obesity and was particularly dramatic in HFD. In HCHD, CB1 blockade by AM251 reduced the Srebf-1 and ChREBP expression and totally abrogated the remnant gene expression of their target lipogenic enzymes. The phosphorylated form of the extracellular signal-regulated kinase (ERK-p), which participates in the CB1-mediated signalling pathway, was markedly present in the PrAT of obese rats. ERK-p was drastically repressed by AM251 indicating that CB1 is actually functional in PrAT of obese animals, though its activation loses the ability to stimulate lipogenesis in PrAT of obese rats. Even so, the remnant expression levels of lipogenic transcription factors found in HCHD-fed rats are still dependent on CB1 activity. Hence, in HCHD-induced obesity, CB1 blockade may help to further potentiate the reduction of lipogenesis in PrAT by means of inducing down-regulation of the ChREBP and Srebf-1 gene expression, and consequently in the expression of lipogenic enzymes.
Published: 2014

66. Molecular and functional characterization of CpACS27A gene reveals its involvement in monoecy instability and other associated traits in squash (Cucurbita pepo L.)

Author: Zoraida Megías, Dolores Garrido, Adnane Boualem, Susana Manzano, Abdelhafid Bendahmane, Alejandro Barrera, Manuel Jamilena, Cecilia Martínez, Universidad de Almería (UAL), Unité de recherche en génomique végétale (URGV), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Universidad de Granada (UGR), grant AGL - ERDF (European Regional Development Fund) [2011-30568-C02-02/ALI], Spanish Ministry of Science and Innovation, ERDF [CVI-02617], Consejeria de Innovacion, Ciencia y Empresa, Junta de Andalucia, Spain, FPU program scholarships from MEC, Spain, Spanish Ministry of Science and Innovation [PTA2011-479-I], and Universidad de Granada = University of Granada (UGR)
Subjects: Gynoecium, Genotype, Pseudogene, [SDV]Life Sciences [q-bio], Molecular Sequence Data, Stamen, Stamen arrest, Flowers, Plant Science, Biology, Cucurbita pepo, Ethylene, Cucurbita, Gene Expression Regulation, Plant, Genetics, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Plant reproductive morphology, Amino Acid Sequence, Cloning, Molecular, Andromonoecy, Gene, Phylogeny, Plant Proteins, Reverse Transcriptase Polymerase Chain Reaction, Reproduction, Unisexual flower development, food and beverages, Ethylenes, Sex determination, biology.organism_classification, Phenotype, Mutation, Parthenocarpy
Abstract: International audience; A number of Cucurbita pepo genotypes showing instable monoecy or partial andromonoecy, i.e. an incomplete conversion of female into bisexual flowers, have been detected. Given that in melon and cucumber andromonoecy is the result of reduction of ethylene production in female floral buds, caused by mutations in the ethylene biosynthesis genes CmACS7 and CsACS2; we have cloned and characterized two related C. pepo genes, CpACS27A and CpACS27B. The molecular structure of CpACS27A and its specific expression in the carpels of female flowers during earlier stages of flower development suggests that this gene is the Cucurbita ortholog of CmACS7 and CsACS2. CpACS27B is likely to be a paralogous pseudogene since it has not been found to be expressed in any of the analyzed tissues. CpACS27A was sequenced in Bolognese (Bog) and Vegetable Spaghetti (Veg), two monoecious inbred lines whose F2 was segregating for partial andromonoecy. The Bog allele of CpACS27A carried a missense mutation that resulted in a substitution of the conserved serine residue in position 176 by an alanine. Segregation analysis indicated that this mutant variant is necessary but not sufficient to confer the andromonoecious phenotype in squash. In concordance with its involvement in stamen arrest, a reduction in CpACS27A expression has been found in bisexual flower buds at earlier stages of development. This reduction in CpACS27A expression was concomitant with a downregulation of other ethylene biosynthesis and signaling genes during earlier and later stages of ovary development. The role of CpACS27A is discussed regarding the regulation of ethylene biosynthesis and signaling genes in the control of andromonoecy-associated traits, such as the delayed maturation of corolla and stigma as well as the parthenocarpic development of the fruit.
Published: 2014
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67. Mid-childhood fat mass and airflow limitation at 15 years:The mediating role of insulin resistance and C-reactive protein

Author: Gabriela P. Peralta, Raquel Granell, Annabelle Bédard, Anne‐Elie Carsin, Elaine Fuertes, Laura D. Howe, Sandra Márquez, Deborah L. Jarvis, Judith Garcia‐Aymerich, CIBER de Epidemiología y Salud Pública (CIBERESP), Instituto de Salud Global - Institute For Global Health [Barcelona] (ISGlobal), Universitat Pompeu Fabra [Barcelona] (UPF), Universität Zürich [Zürich] = University of Zurich (UZH), University of Bristol [Bristol], Centre de recherche en épidémiologie et santé des populations (CESP), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, Hospital del Mar Medical Research Institute [Barcelona, Spain] (IMIM), Imperial College London, Wellcome Trust, WT, Medical Research Council, MRC: 076467/Z/05/Z, G0401540/73080, Generalitat de Catalunya, Ministerio de Ciencia e Innovación, MICINN, Horizon 2020: 633212, The present analyses are part of the Aging Lungs in European Cohorts (ALEC) Study ( www.alecstudy.org ), which has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement No. 633212. The content of this article reflects only the authors' views, and the European Commission is not liable for any use that may be made of the information contained therein. The UK Medical Research Council and Wellcome (Grant reference: 217065/Z/19/Z) and the University of Bristol provide core support for ALSPAC. This publication is the work of the authors and Laura Howe and Raquel Granell will serve as guarantors for the contents of this paper. A comprehensive list of grant funding is available on the ALSPAC website ( http://www.bristol.ac.uk/alspac/external/documents/grant‐acknowledgements.pdf ). Specifically, grants from Wellcome Trust and MRC (076467/Z/05/Z and G0401540/73080) supported the collection of body composition and lung function data at 15 years. We acknowledge support from the Spanish Ministry of Science and Innovation through the 'Centro de Excelencia Severo Ochoa 2019–2023' Program (CEX2018‐000806‐S), and support from the Generalitat de Catalunya through the CERCA Program., European Project: 633212,H2020,H2020-PHC-2014-two-stage,ALEC(2015), HAL UVSQ, Équipe, and Aging Lungs in European Cohorts - ALEC - - H20202015-05-01 - 2019-04-30 - 633212 - VALID
Subjects: [SDV.MHEP.PED]Life Sciences [q-bio]/Human health and pathology/Pediatrics, obesity, Immunology, ALSPAC, C-reactive protein, [SDV.MHEP.PED] Life Sciences [q-bio]/Human health and pathology/Pediatrics, insulin resistance, Pediatrics, Perinatology and Child Health, Immunology and Allergy, epidemiology, mediation, airflow limitation
Abstract: Key MessageAlthough obesity measures have been consistently associated with a lower FEV1/FVC ratio in adolescents, no previous study has assessed the underlying mechanisms of this association This population-based study assessed whether insulin resistance and CRP levels at 15 years partially mediate the association between fat mass and FEV1/FVC using a causal mediation analysis approach. The findings suggest that insulin resistance at 15 years may mediate over 20% of this association, but no evidence of a mediating role of CRP was found. Further, longitudinal studies that evaluate other biomarkers of systemic inflammation and examine other potential mechanisms are needed to better understand the pathways linking obesity and respiratory health in adolescence. This is key for public health interventions and targeting clinical interventions.AbstractBackgroundWe previously reported an association of high fat mass levels from age 9 to 15 years with lower forced expiratory flow in 1 s (FEV1)/forced vital capacity (FVC) ratio (i.e., increased risk of airflow limitation) at 15 years. Here, we aimed to assess whether insulin resistance and C-reactive protein (CRP) at 15 years partially mediate this association.MethodsWe included 2263 children from the UK Avon Longitudinal Study of Parents and Children population-based cohort (ALSPAC). Four fat mass index (FMI) trajectories (“low,” “medium-low,” “medium-high,” “high”) from 9 to 15 years were previously identified using Group-Based Trajectory Modeling. Data on CRP, glucose, insulin, and post-bronchodilator FEV1/FVC were available at 15 years. We defined insulin resistance by the homeostasis model assessment-estimated insulin resistance index (HOMA-IR). We used adjusted linear regression models and a causal mediation analysis to assess the mediating role of HOMA-IR and CRP.ResultsCompared to children in the “low” FMI trajectory, children in the “medium-high” and “high” FMI trajectories had lower FEV1/FVC at 15 years. The percentage of the total effect explained by HOMA-IR was 19.8% [−114.1 to 170.0] and 20.4% [1.6 to 69.0] for the “medium-high” and “high” trajectories, respectively. In contrast, there was little evidence for a mediating role of CRP.ConclusionThe association between mid-childhood fat mass and FEV1/FVC ratio at 15 years may be partially mediated by insulin resistance.
Published: 2022
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68. Genomic characterization of Listeria spp. isolated from tonsils, udder and feces of domestic dairy ruminants in Spain

Author: Carla Palacios-Gorba, Alexandra Moura, Yuval Markovich, Nathalie Tessaud-Rita, Ángel Gómez-Martín, Hélène Bracq-Dieye, Jesús Gomis, Guillaume Vales, María Pastor-Martín, Pierre Thouvenot, Carles Escrig, Alexandre Leclercq, Marc Lecuit, Juan J. Quereda, Universidad Cardenal Herrera-CEU (CEU-UCH), Biologie des Infections - Biology of Infection, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Centre National de Référence Listeria - National Reference Center Listeria (CNR), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO)-Université Paris Cité (UPCité), Generalitat Valenciana, CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), and This work was supported by Generalitat Valenciana (Project reference AICO/2021/278) (JJQ), the Spanish Ministry of Science and Innovation (Project references PID2019-110764RA-I00/AEI/10.13039/501100011033 (JJQ) and PID2020-119462RA-I00/AEI/10.13039/501100011033 (AGM)) and Universidad CEU Cardenal Herrera Programa INDI 20/40 (JJQ), Institut Pasteur, Inserm, and Santé Publique France (ML). J.J. Quereda is supported by a 'Ramón y Cajal' contract of the Spanish Ministry of Science, Innovation, and Universities (RYC-2018-024985-I). C. Palacios-Gorba and Yuval Markovich are supported by a Predoctoral contract from the Universidad Cardenal Herrera-CEU.
Subjects: [SDV]Life Sciences [q-bio], Immunology, ruminant, Microbiology, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Infectious Diseases, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, udder, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, tonsil, dairy, Listeria spp, WGS, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: International audience; Two species of Listeria are pathogenic, Listeria monocytogenes and Listeria ivanovii. Although studies have shown that dairy ruminants shed Listeria spp. in feces, there is little information about ruminants that do not shed Listeria spp. in their feces but asymptomatically carry them in organs. We evidence that ruminants can asymptomatically carry L. ivanovii in udders and L. monocytogenes and L. ivanovii in tonsils without fecal shedding. Whole-genome sequence of L. monocytogenes and L. ivanovii contained known core genes involved in virulence and antibiotic resistance. This work highlights tonsils and udders as a Listeria intra-host site of colonization.
Published: 2022
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69. Parallel evolution of amphioxus and vertebrate small-scale gene duplications

Author: Marina Brasó-Vives, Ferdinand Marlétaz, Amina Echchiki, Federica Mantica, Rafael D. Acemel, José L. Gómez-Skarmeta, Diego A. Hartasánchez, Lorlane L. Targa, Pierre Pontarotti, Juan J. Tena, Ignacio Maeso, Hector Escriva, Manuel Irimia, Marc Robinson-Rechavi, Department of Ecology and Evolution [Lausanne], Université de Lausanne = University of Lausanne (UNIL), Dept of Genetics, Evolution and Environment [London] (UCL-GEE), University College of London [London] (UCL), Centre for Genomic Regulation [Barcelona] (CRG), Universitat Pompeu Fabra [Barcelona] (UPF)-Centro Nacional de Analisis Genomico [Barcelona] (CNAG), Andalusian Centre for Developmental Biology (CABD), Department of Computational Biology, Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Department of Genetics, Microbiology and Statistics, and IRBio, University of Barcelona, Barcelona, Spain, Biologie intégrative des organismes marins (BIOM), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Observatoire océanologique de Banyuls (OOB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Swiss Institute of Bioinformatics [Lausanne] (SIB), MRR acknowledges support from the Swiss National Science Foundation grant 31003A/173048, and from the Etat de Vaud. FMar acknowledges support from the Royal Society grant URF\R1\191161. MI acknowledges support from European Research Council ERC‑StG‑LS2‑637591. HE acknowledges support from the Centre National de la Recherche Scientifique and Agence Nationale de la Recherche (ANR‑16‑CE12‑0008‑01 and ANR‑19‑CE13‑0011). PP acknowledges support from the French Government under the 'Investissements d’avenir' program managed by the Agence Nationale de la Recherche (Méditerranée Infection 10‑IAHU‑03). IM acknowledges support from the Spanish Ministry of Science and Innovation and the European Union (RYC‑2016‑20089 and PGC2018‑099392‑A‑I00). JJT acknowledges support from the ERC (Grant Agreement No. 740041) and the Spanish Ministerio de Ciencia e Innovación (PID2019‑103921GB‑I00)., ANR-16-CE12-0008,CHORELAND,Détermination de la conservation du landscape génomique de régulation au cours de l'embryogenèse des chordés(2016), ANR-19-CE13-0011,NEUCECHO,Dynamique de l'induction neurale au niveau de la cellule unique chez les chordés(2019), Swiss National Science Foundation, Royal Society (UK), European Research Council, Centre National de la Recherche Scientifique (France), Agence Nationale de la Recherche (France), Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), and Agencia Estatal de Investigación (España)
Subjects: animal structures, Amphioxus, Genome assembly, Gene duplication, Vertebrate, Comparative genomics, [SDV]Life Sciences [q-bio], Genomics, Animals, Lancelets/genetics, Gene Duplication, Phylogeny, Vertebrates/genetics, Vertebrates/metabolism, Evolution, Molecular, Branchiostoma lanceolatum, Ohnolog, Small-scale duplication, Genòmica, Amfiox, Genes, Vertebrats, Vertebrates, Gens, Lancelets
Abstract: [Background]: Amphioxus are non-vertebrate chordates characterized by a slow morphological and molecular evolution. They share the basic chordate body-plan and genome organization with vertebrates but lack their 2R whole-genome duplications and their developmental complexity. For these reasons, amphioxus are frequently used as an outgroup to study vertebrate genome evolution and Evo-Devo. Aside from whole-genome duplications, genes continuously duplicate on a smaller scale. Small-scale duplicated genes can be found in both amphioxus and vertebrate genomes, while only the vertebrate genomes have duplicated genes product of their 2R whole-genome duplications. Here, we explore the history of small-scale gene duplications in the amphioxus lineage and compare it to small- and large-scale gene duplication history in vertebrates. [Results]: We present a study of the European amphioxus (Branchiostoma lanceolatum) gene duplications thanks to a new, high-quality genome reference. We find that, despite its overall slow molecular evolution, the amphioxus lineage has had a history of small-scale duplications similar to the one observed in vertebrates. We find parallel gene duplication profiles between amphioxus and vertebrates and conserved functional constraints in gene duplication. Moreover, amphioxus gene duplicates show levels of expression and patterns of functional specialization similar to the ones observed in vertebrate duplicated genes. We also find strong conservation of gene synteny between two distant amphioxus species, B. lanceolatum and B. floridae, with two major chromosomal rearrangements. [Conclusions]: In contrast to their slower molecular and morphological evolution, amphioxus’ small-scale gene duplication history resembles that of the vertebrate lineage both in quantitative and in functional terms., RR acknowledges support from the Swiss National Science Foundation grant 31003A/173048, and from the Etat de Vaud. FMar acknowledges support from the Royal Society grant URF\R1\191161. MI acknowledges support from European Research Council ERC-StG-LS2-637591. HE acknowledges support from the Centre National de la Recherche Scientifique and Agence Nationale de la Recherche (ANR-16-CE12-0008-01 and ANR-19-CE13-0011). PP acknowledges support from the French Government under the “Investissements d’avenir” program managed by the Agence Nationale de la Recherche (Méditerranée Infection 10-IAHU-03). IM acknowledges support from the Spanish Ministry of Science and Innovation and the European Union (RYC-2016-20089 and PGC2018-099392-A-I00). JJT acknowledges support from the ERC (Grant Agreement No. 740041) and the Spanish Ministerio de Ciencia e Innovación (PID2019-103921GB-I00).
Published: 2022
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70. Considering waning immunity to better explain dengue dynamics

Author: Leonardo López, Richard E. Paul, Van-Mai Cao-Lormeau, Xavier Rodó, Instituto de Salud Global - Institute For Global Health [Barcelona] (ISGlobal), Écologie et Émergence des Pathogènes Transmis par les Arthropodes / Ecology and Emergence of Arthropod-borne Pathogens, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Institut Louis Malardé [Papeete] (ILM), Institut de Recherche pour le Développement (IRD), Institució Catalana de Recerca i Estudis Avançats (ICREA), XR and LL acknowledge support from the Spanish Ministry of Science and Innovation through the ‘Centro de Excelencia Severo Ochoa 2019 2023’ Program (CEX2018 000806S), and support from the Generalitat de Catalunya, Spain through the CERCA Program. The research leading to these results has received funding from the European Commission Seventh Framework Program for the DENFREE project under Grant Agreement n282378 to RP, XR, VMCL., and European Project: 282378,EC:FP7:HEALTH,FP7-HEALTH-2011-single-stage,DENFREE(2012)
Subjects: DENV, French, Epidemiology, [SDV]Life Sciences [q-bio], Public Health, Environmental and Occupational Health, Monotypic, Immunity, Microbiology, Polynesia, Dengue, Infectious Diseases, Virology, Parasitology, Epidemics, Vaccine, Model
Abstract: International audience; Lifelong serotype-specific immunity following dengue virus infection may not always occur, but the true extent of this effect is unknown. Analysis of more than 20 years of monotypic epidemics in the isolated French Polynesian islands revealed that whilst the risk of symptomatic dengue infection did conform to the classical paradigms of homotypic immunity and increased disease risk in heterotypic secondary infections, incorporation of waning immunity improved the ability of epidemiological models to capture the observed epidemic dynamics. Not only does this show how inclusion of waning immunity into classical models can reveal important facets of the immune response to natural dengue virus infection, it also has significant ramifications for vaccine development and implementation in dengue endemic areas.
Published: 2022
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71. Discovering HIV related information by means of association rules and machine learning

Author: Víctor Asensi Álvarez, Lourdes Araujo, DAVID DALMAU, MARIA REMEDIOS ALEMAN VALLS, Rocio Montejano Sanchez, Alejandro Gonzalez-Serna, Joaquín Bravo Urbieta, Víctor Hontañón Antoñana, Ministerio de Ciencia e Innovación (España), Instituto de Salud Carlos III, Red de Investigación Cooperativa en Investigación en Sida (España), Plan Nacional de I+D+i (España), Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF), [Araujo, Lourdes] ETS Ingn Informat UNED, Languages & Informat Syst Dept, Juan Del Rosal 16, Madrid 28040, Spain, [Martinez-Romo, Juan] ETS Ingn Informat UNED, Languages & Informat Syst Dept, Juan Del Rosal 16, Madrid 28040, Spain, [Bisbal, Otilia] Hosp Univ 12 Octubre, Inst Invest I 12, Madrid, Spain, [Sanchez-de-Madariaga, Ricardo] Inst Salud Carlos III, Telemed & Hlth Res Unit, Madrid 28029, Spain, [Araujo, Lourdes] Inst Mixto UNED ISCIII MIENS, Madrid 28029, Spain, [Martinez-Romo, Juan] Inst Mixto UNED ISCIII MIENS, Madrid 28029, Spain, [Sanchez-de-Madariaga, Ricardo] Inst Mixto UNED ISCIII MIENS, Madrid 28029, Spain, Spanish Ministry of Science and Innovation, OBSER-MENH Project (MCIN/AEI), UE ('NextGenerationEU'/PRTR), project RAICES (IMIENS 2022), Instituto de Salud Carlos III through the Red Tematica de Investigacion Cooperativa en Sida, and ISCIII-Subdireccion General de Evaluacion and el Fondo Europeo de Desarrollo Regional (FEDER)
Subjects: Machine Learning, Acquired Immunodeficiency Syndrome, Multidisciplinary, Quality of Life, Humans, HIV Infections
Abstract: Acquired immunodeficiency syndrome (AIDS) is still one of the main health problems worldwide. It is therefore essential to keep making progress in improving the prognosis and quality of life of affected patients. One way to advance along this pathway is to uncover connections between other disorders associated with HIV/AIDS-so that they can be anticipated and possibly mitigated. We propose to achieve this by using Association Rules (ARs). They allow us to represent the dependencies between a number of diseases and other specific diseases. However, classical techniques systematically generate every AR meeting some minimal conditions on data frequency, hence generating a vast amount of uninteresting ARs, which need to be filtered out. The lack of manually annotated ARs has favored unsupervised filtering, even though they produce limited results. In this paper, we propose a semi-supervised system, able to identify relevant ARs among HIV-related diseases with a minimal amount of annotated training data. Our system has been able to extract a good number of relationships between HIV-related diseases that have been previously detected in the literature but are scattered and are often little known. Furthermore, a number of plausible new relationships have shown up which deserve further investigation by qualified medical experts. This study has been partially supported by the Spanish Ministry of Science and Innovation within the DOTTHEALTH Project (MCI/AEI/FEDER, UE) under Grant PID2019-106942RB-C32, the OBSER-MENH Project (MCIN/AEI/10.13039/501100011033 and UE (“NextGenerationEU”/PRTR)) under Grant TED2021-130398B-C21 and the project RAICES (IMIENS 2022), PI18CIII/00004 “Infobanco para uso secundario de datos basado en estándares de tecnología y conocimiento: implementación y evaluación de un infobanco de salud para CoRIS (Info-bank for the secondary use of data based on technology and knowledge standards: implementation and evaluation of a health info-bank for CoRIS) - SmartPITeS” and PI18CIII/00019 - PI18/00890 - PI18/00981 “Arquitectura normalizada de datos clínicos para la generación de infobancos y su uso secundario en investigación: solución tecnológica (Clinical data normalized architecture for the generation of info-banks and their secondary use in research: technological solution) - CAMAMA 4” from Fondo de Investigación Sanitaria (FIS) Plan Nacional de I+D+i. The RIS cohort (CoRIS) is supported by the Instituto de Salud Carlos III through the Red Temática de Investigación Cooperativa en Sida (RD06/006, RD12/0017/0018 and RD16/0002/0006) as part of the Plan Nacional R+D+I and co-financed by ISCIII-Subdirección General de Evaluación and el Fondo Europeo de Desarrollo Regional (FEDER). The list of members of the Cohort of the Spanish HIV Research Network (CoRIS) is included in the Supplementary Material. Additional relationships between HIV-related diseases confirmed or discarded are included as Supplementary Material. This study would not have been possible without the collaboration of all patients, medical and nursing staff and data mangers who have taken part in the Project. Sí
Published: 2022

72. Update on the Cenomanian vertebrates from Algora (Guadalajara, Spain):new data on the faunal transition between the Early and the Late Cretaceous of Europe

Author: Pérez García, Adán, Cabezuelo Hernández, Alberto, Guerrero, Andréa, Martín Jiménez, Marcos, Mocho, Pedro, Narváez, Iván, Ortega, Francisco, Torices, Angelica, Vullo, Romain, UNED - Universidad Nacional de Educación a Distancia, Madrid, Spain, Universidade de Lisboa, Natural History Museum of Los Angeles County, Universidad Complutense de Madrid = Complutense University of Madrid [Madrid] (UCM), Géosciences Rennes (GR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), Spanish Ministry of Science and Innovation (PID2019-111488RB-I00), and Regional Government of Castile-La Mancha (SBPLY/19/180801/000034 and SBPLY/21/180801/000039).
Subjects: Vertebrate palaeontology, Upper Cretaceous, Reptiles, Iberian record, Faunal transition, [SDU.STU.PG]Sciences of the Universe [physics]/Earth Sciences/Paleontology
Abstract: International audience; The largest concentration of vertebrate macroremains for the Cenomanian of south-westernEurope has been recognized at the palaeontological area of Algora (Guadalajara Province, centralSpain). The study of the taxa identified there provided new information about the palaeobiogeographyand temporal distributions of some lineages, and increased knowledge about the poorly-known transition between the Lower and the Upper Cretaceous faunas in Europe. The presenceof eight taxa was confirmed there: the lepisosteoid fish Obaichthys africanus; the helochelydridturtle aff. Plastremys lata; the bothremydid turtle Algorachelus peregrina; an indeterminate elasmosauridplesiosaur; a non-eusuchian neosuchian and a eusuchian crocodyliform; an indeterminatelithostrotian titanosaur; and an indeterminate theropod probably belonging to Abelisauridae.Recent fieldwork campaigns, performed in 2019 and 2021, provided more than 1,200 new vertebrateremains, including those of clades poorly represented so far, as well as others hithertounknown at this site. Therefore, the faunal list can be remarkably increased. In this sense, severaltaxa of Osteichthyes have been found, and chondrichthyan remains are also recognized there forthe first time. New remains of crocodyliforms, theropods and sauropods allow to analyze, in moredetail, the systematic attribution of the representatives of these lineages present in Algora. Notonly new relevant specimens of helochelydrids and bothremydids stand out among the numerousfinds of turtles (which allow a better understanding of the anatomy of the represented species),but especially the discovery of the partial skeleton of a relatively large cryptodiran sea turtle(including, among other elements, the carapace, with more than 1 meter in length, and the skull).In addition, other lineages hitherto unrecognized at this fossiliferous locality, such as a squamatewith aquatic affinities, are identified. The main novelties in knowledge about the Cenomanian vertebratefauna of Algora are presented here, and the palaeobiogeographical implications of severalof the new finds are discussed.
Published: 2022

73. Yeast ribosomal protein L7 and its homologue Rlp7 are simultaneously present at distinct sites on pre-60S ribosomal particles

Author: Gwenael Badis, Cosmin Saveanu, Antonio Díaz-Quintana, Alain Jacquier, Abdelkader Namane, Reyes Babiano, Micheline Fromont-Racine, Antonia Doyen, Jesús de la Cruz, Departamento de Genética, Génétique des Interactions macromoléculaires, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Universidad de Sevilla, Spanish Ministry of Science and Innovation and ERDF[BFU2010-15690 and FR2009-0102], AndalusianGovernment [CVI-271 and P08-CVI-03508 to J.d.l.C.], Agence Nationale de la Recherche [ANR-2011-BSV6-011-785 02], EGIDE Picasso Programme (to M.F-R.), recipient of an FPI fellowship from the Andalusian Government (to R.B.). Funding for open access charge:Spanish Ministry of Science and Innovation and ERDF[BFU2010-15690 to J.d.l.C.]., ANR-11-BSV6-0011,NGD-NSD,Rôle du NSD/NGD: identification des gènes cibles et des facteurs impliqués dans ces mécanismes chez Saccharomyces cerevisiae(2011), Génétique des Interactions macromoléculaires / Genetics of Macromolecular Interactions, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), and Universidad de Sevilla / University of Sevilla
Subjects: Ribosomal Proteins, 5S, Saccharomyces cerevisiae Proteins, [SDV]Life Sciences [q-bio], Molecular Sequence Data, Biology, Ribosome assembly, 03 medical and health sciences, 5S ribosomal RNA, Ribosomal protein, Genetics, RNA Precursors, Ribosome Subunits, Eukaryotic Small Ribosomal Subunit, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, 030304 developmental biology, 50S, Ribosomal, 0303 health sciences, Binding Sites, Base Sequence, Eukaryotic Large Ribosomal Subunit, 030302 biochemistry & molecular biology, RNA, Ribosomal, 5S, Ribosome Subunits, Large, Eukaryotic, Ribosomal RNA, Molecular biology, Cell biology, RNA, Ribosomal, Large, Eukaryotic, RNA, Eukaryotic Ribosome
Abstract: International audience; Ribosome biogenesis requires \textgreater300 assembly factors in Saccharomyces cerevisiae. Ribosome assembly factors Imp3, Mrt4, Rlp7 and Rlp24 have sequence similarity to ribosomal proteins S9, P0, L7 and L24, suggesting that these pre-ribosomal factors could be placeholders that prevent premature assembly of the corresponding ribosomal proteins to nascent ribosomes. However, we found L7 to be a highly specific component of Rlp7-associated complexes, revealing that the two proteins can bind simultaneously to pre-ribosomal particles. Cross-linking and cDNA analysis experiments showed that Rlp7 binds to the ITS2 region of 27S pre-rRNAs, at two sites, in helix III and in a region adjacent to the pre-rRNA processing sites C1 and E. However, L7 binds to mature 25S and 5S rRNAs and cross-linked predominantly to helix ES7(L)b within 25S rRNA. Thus, despite their predicted structural similarity, our data show that Rlp7 and L7 clearly bind at different positions on the same pre-60S particles. Our results also suggest that Rlp7 facilitates the formation of the hairpin structure of ITS2 during 60S ribosomal subunit maturation.
Published: 2013
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74. Inactivation of the panE gene in Lactococcus lactis enhances formation of cheese aroma compounds

Author: Luz P. Gómez de Cadiñanos, Mireille Yvon, Teresa Requena, Tomás García-Cayuela, Carmen Peláez, M. Carmen Martínez-Cuesta, Ministerio de Ciencia e Innovación (España), Comunidad de Madrid, Institut National de la Recherche Agronomique (INRA), Spanish Ministry of Science and Innovation [AGL2006-12100, AGL2009-13361-C02-02, RM2011-00003-00-00], Spanish Ministry of Science and Innovation (Consolider Ingenio) [FUN-C-FOOD-CSD2007-00063], and Comunidad de Madrid [ALIBIRD P2009/AGR-1469]
Subjects: AMINOTRANSFERASE, GRAM-POSITIVE BACTERIA, [SDV]Life Sciences [q-bio], Dehydrogenase, Applied Microbiology and Biotechnology, Polymerase Chain Reaction, Gas Chromatography-Mass Spectrometry, FLAVOR COMPOUNDS, Cheese, Food science, Gene Silencing, Gene, Aroma, DNA Primers, Aldehydes, Ecology, biology, Strain (chemistry), AMINO-ACCATABOLISM, Lactococcus lactis, PATHWAYS, food and beverages, biology.organism_classification, Enzyme assay, Alcohol Oxidoreductases, Biochemistry, METHIONINE CATABOLISM, Odorants, biology.protein, Food Microbiology, Food Technology, Food Science, Biotechnology
Abstract: Hydroxyacid dehydrogenases limit the conversion of α-keto acids into aroma compounds. Here we report that inactivation of the panE gene, encoding the α-hydroxyacid dehydrogenase activity in Lactococcus lactis, enhanced the formation of 3-methylbutanal and 3-methylbutanol. L. lactis IFPL953ΔpanE was an efficient strain producing volatile compounds related to cheese aroma., This work was supported by the Spanish Ministry of Science and Innovation (grants AGL2006-12100, AGL2009-13361-C02-02, RM2011-00003-00-00, and Consolider Ingenio 2010 FUN-C-FOOD-CSD2007-00063) and Comunidad de Madrid (grant ALIBIRD P2009/AGR-1469).
Published: 2013
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75. Atmospheric turbulence triggers pronounced diel pattern in karst carbonate geochemistry

Author: Yves Goddéris, J. Schott, R. Van Grieken, Francisco Domingo, Philippe Ciais, Andrew S. Kowalski, Penélope Serrano-Ortiz, Dan Yakir, Ivan A. Janssens, Enrique P. Sánchez-Cañete, Sergio Sanchez-Moral, Soledad Cuezva, Bernard Longdoz, Marilyn Roland, Carolina Cardell, Department of Biology (University of Antwerp), University of Antwerp (UA), Estación Experimental de Zonas Aridas, Consejo Superior de Investigaciones Científicas [Spain] (CSIC), Centro Andaluz de Medio Ambiente (CEAMA), Universidad de Granada (UGR), Departamento de Fisica Aplicada [Granada], Géosciences Environnement Toulouse (GET), Centre National de la Recherche Scientifique (CNRS)-Observatoire Midi-Pyrénées (OMP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Recherche pour le Développement (IRD)-Centre National d'Études Spatiales [Toulouse] (CNES), Laboratoire des Sciences du Climat et de l'Environnement [Gif-sur-Yvette] (LSCE), Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Centre National de la Recherche Scientifique (CNRS), Departamento de Ciencias de la Tierra y del Medio Ambiente [Universidad de Alicante], Universidad de Alicante, Museo Nacional de Ciencias Naturales, Ecologie et Ecophysiologie Forestières [devient SILVA en 2018] (EEF), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Weizmann Institute of Science, Universiteit Antwerpen [Antwerpen], Departamento de Mineralogia y Petrologia [Granada], Institute for Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen), Spanish Ministry of Science and Innovation, Spanish Ministry of Science and Innovation, research programme Juan de la Cierva, Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Observatoire Midi-Pyrénées (OMP), Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD), Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), ICOS-ATC (ICOS-ATC), Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Weizmann Institute of Science [Rehovot, Israël], Universidad de Alicante. Departamento de Ciencias de la Tierra y del Medio Ambiente, Petrología Aplicada, Universidad de Granada = University of Granada (UGR), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Midi-Pyrénées (OMP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Centre National de la Recherche Scientifique (CNRS), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Museo Nacional de Ciencias Naturales [Madrid] (MNCN), and Universiteit Antwerpen = University of Antwerpen [Antwerpen]
Subjects: 010504 meteorology & atmospheric sciences, [SDV]Life Sciences [q-bio], Karst carbonate geochemistry, lcsh:Life, Weathering, UNSATURATED ZONE, 010501 environmental sciences, Atmospheric sciences, 01 natural sciences, DESERT, Atmosphere, chemistry.chemical_compound, DISSOLUTION, lcsh:QH540-549.5, ROCK FRAGMENTS, Ecosystem, Precipitation, Biology, Ecology, Evolution, Behavior and Systematics, 0105 earth and related environmental sciences, Earth-Surface Processes, WATER-VAPOR ADSORPTION, Diel pattern, Physics, SPAIN, lcsh:QE1-996.5, 04 agricultural and veterinary sciences, Soil carbon, 15. Life on land, CO2 FLUX, NET ECOSYSTEM, SOIL CARBON, DIOXIDE, lcsh:Geology, Chemistry, lcsh:QH501-531, Outgassing, Oceanography, chemistry, 13. Climate action, Atmospheric turbulence triggers, 040103 agronomy & agriculture, 0401 agriculture, forestry, and fisheries, Carbonate, Terrestrial ecosystem, lcsh:Ecology, Geology, Petrología y Geoquímica
Abstract: CO2 exchange between terrestrial ecosystems and the atmosphere is key to understanding the feedbacks between climate change and the land surface. In regions with carbonaceous parent material, CO2 exchange patterns occur that cannot be explained by biological processes, such as disproportionate outgassing during the daytime or nighttime CO2 uptake during periods when all vegetation is senescent. Neither of these phenomena can be attributed to carbonate weathering reactions, since their CO2 exchange rates are too small. Soil ventilation induced by high atmospheric turbulence is found to explain atypical CO2 exchange between carbonaceous systems and the atmosphere. However, by strongly altering subsurface CO2 concentrations, ventilation can be expected to influence carbonate weathering rates. By imposing ventilation-driven CO2 outgassing in a carbonate weathering model, we show here that carbonate geochemistry is accelerated and does play a surprisingly large role in the observed CO2 exchange pattern of a semi-arid ecosystem. We found that by rapidly depleting soil CO2 during the daytime, ventilation disturbs soil carbonate equilibria and therefore strongly magnifies daytime carbonate precipitation and associated CO2 production. At night, ventilation ceases and the depleted CO2 concentrations increase steadily. Dissolution of carbonate is now enhanced, which consumes CO2 and largely compensates for the enhanced daytime carbonate precipitation. This is why only a relatively small effect on global carbonate weathering rates is to be expected. On the short term, however, ventilation has a drastic effect on synoptic carbonate weathering rates, resulting in a pronounced diel pattern that exacerbates the non-biological behavior of soil–atmosphere CO2 exchanges in dry regions \mbox{with carbonate soils}., M. Roland was granted by the Institute for Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen). I. A. Janssens and R. Van Grieken acknowledge the Research Foundation – Flanders (FWO). P. Serrano-Ortiz is funded by a postdoctoral fellowship from the Spanish Ministry of Science and Innovation. S. Cuezva was funded by a postdoctoral fellowship from the Spanish Ministry of Science and Innovation, research programme Juan de la Cierva.
Published: 2013
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76. Crack tip solution for Mode III cracks in spring interfaces

Author: Sara Jiménez-Alfaro, Vladislav Mantič, Universidad de Sevilla. Departamento de Mecánica de Medios Continuos y Teoría de Estructuras, Universidad de Sevilla. TEP131: Elasticidad y Resistencia de Materiales, Spanish Ministry of Science and Innovation PID2021-123325OB-I00, Consejería de Económía, Conocimiento, Empresas Universidad, Junta de Andalucía and European Regional Development Fund P18-FR-1928, and European Union’s Horizon 2020 research and innovation programme under Marie Sklodowska-Curie grant agreement No. 861061- NEWFRAC
Subjects: Antiplane strain, Mechanics of Materials, Adhesive interface, Mechanical Engineering, Logarithmic stress singularity, General Materials Science, Interface fracture, Asymptotic solution
Abstract: Considering an infinite linear elastic isotropic solid in antiplane shear, the Mode III crack-tip solution for a semi-infinite crack located in a straight spring interface is systematically studied for the first time. A new analytic expression for this crack-tip solution is given in the form of a double asymptotic series of the main and the so-called associated shadow terms. It is shown that the series of the shadow terms associated with a main term is infinite, and all shadow terms include logarithmic terms. Thus, although the interface tractions are bounded, the linear elastic solution at this crack-tip has a logarithmic stress singularity which is comprehensively analysed. Noteworthy, the character of this stress singularity is very different from the well-known square root singularity at the crack tip in the classical fracture mechanics. A key advantage of the present approach is its simplicity, as only elementary mathematical tools are employed, and also its easy implementation in a computer algebra software. The latter fact is very relevant because the expressions of higher-order shadow terms become increasingly complicated, so their generation by a computer code becomes crucial. The present results allow the implementation of new enriched or singular crack-tip finite elements for such cracks, and the automatic generation of analytic solutions for benchmark problems for testing the finite-element codes using these special elements. Such codes can be applied to efficient numerical modelling of interface cracks, e.g., in adhesively bonded joints with a thin adhesive layer.
Published: 2023
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77. Mechanical properties of high-G·C content DNA with A-type base-stacking

Author: Hormeño S., Ibarra B., Carrascosa J.L., Valpuesta J.M., Moreno-Herrero F., Arias-Gonzalez J.R. and This work was supported by grants from the Spanish Ministry of Science and Innovation (No. BFU2010-15703/BMC, No. BFU2008-02328/BMC, and Consolider No. CSD2007-00010) and the Comunidad de Madrid (No. S2009/MAT/1507). S.H. acknowledges a scholarship from Consejería de Educación de la Comunidad de Madrid and the European Social Fund. J.R.A.-G. acknowledges a former individual fellowship from the International Human Frontier Science Program Organization and a Ramón y Cajal contract from the Spanish Ministry of Science and Innovation. Work in F.M.-H. Laboratory is supported by the European Research Council (starting grant No. 206117) and the Spanish Ministry of Science and Innovation (grant No. FIS2008-0025).
Published: 2011

78. Condensation prevails over B-A transition in the structure of DNA at low humidity

Author: Hormeño S., Moreno-Herrero F., Ibarra B., Carrascosa J.L., Valpuesta J.M., Arias-Gonzalez J.R. and This work was supported by grants from the Spanish Ministry of Science and Innovation (CSD2007-00010, BFU2010-15703/BMC, and BFU2008-02328/BMC) and the Comunidad de Madrid (S-0505/MAT/0283). S.H. acknowledges a scholarship from Consejería de Educación de la Comunidad de Madrid and the European Social Fund. J.R.A.-G. acknowledges a former individual fellowship from the International Human Frontier Science Program Organization and a Ramón y Cajal contract from the Spanish Ministry of Science and Innovation. Work in the F.M.-H. laboratory is supported by a Starting Grant from the European Research Council (no. 206117) and a grant from the Spanish Ministry of Science and Innovation (no. FIS2008-0025).
Published: 2011

79. Fluid deformation in isotropic Darcy flow

Author: Daniel R. Lester, Marco Dentz, Aditya Bandopadhyay, Tanguy Le Borgne, Royal Melbourne Institute of Technology University (RMIT University), Consejo Superior de Investigaciones Científicas [Spain] (CSIC), Indian Institute of Technology Kharagpur (IIT Kharagpur), Géosciences Rennes (GR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), European Research Council [648377], Spanish Ministry of Science and Innovation [PID2019-106887GB-C31], and European Project: 648377,H2020,ERC-2014-CoG,ReactiveFronts(2015)
Subjects: [SPI]Engineering Sciences [physics], Mechanics of Materials, Mechanical Engineering, Applied Mathematics, Porous media, Ensure access to affordable, reliable, sustainable and modern energy for all, Condensed Matter Physics, Mixing and dispersion
Abstract: The deformation of fluid elements governs many processes in porous media, including the transport, dispersion and mixing of solutes, chemical reactions and colloidal particles. Recently, it has been shown that even in strongly heterogeneous porous media, steady three-dimensional (3-D) Darcy flow with isotropic hydraulic conductivity admits only constrained kinematics. This is due to its inherently helicity-free nature, as the velocity is everywhere orthogonal to the vorticity. This property precludes braided streamlines and instead admits a pair of coherent 3-D streamfunctions, and the streamlines cannot wander freely throughout the flow domain. In this study, we consider the impact of these kinematic constraints upon fluid deformation at the Darcy scale. We show that the helicity-free condition corresponds to an orthogonal 3-D streamline coordinate system, which we use to derive an ab initio continuous time random walk framework for fluid deformation. We find that the helicity-free condition combined with the intermittent nature of shear events leads to fluid deformation that is limited to algebraic growth, with stretching ranging from sublinear to superlinear behaviour. Fluid deformation in 3-D isotropic Darcy flow is remarkably similar to that of two-dimensional (2-D) Darcy flow, and the structure of 3-D Darcy flow is fundamentally the same as two superposed 2-D Darcy flows. These results have implications for understanding flow and transport in heterogeneous porous media, and provide a basis for quantification of mixing and dispersion in isotropic 3-D Darcy flow., This work was supported by the European Research Council (T.L.B., grant no. 648377) and the Spanish Ministry of Science and Innovation (M.D., grant no. PID2019-106887GB-C31).
Published: 2022
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80. The Single Residue K12 Governs the Exceptional Voltage Sensitivity of Mitochondrial Voltage-Dependent Anion Channel Gating

Author: Van A. Ngo, María Queralt-Martín, Farha Khan, Lucie Bergdoll, Jeff Abramson, Sergey M. Bezrukov, Tatiana K. Rostovtseva, David P. Hoogerheide, Sergei Yu. Noskov, University of Calgary, Oak Ridge National Laboratory [Oak Ridge] (ORNL), UT-Battelle, LLC, Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), Universitat Jaume I, University of California [Los Angeles] (UCLA), University of California (UC), Laboratoire d'ingénierie des systèmes macromoléculaires (LISM), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), National Institute of Standards and Technology [Gaithersburg] (NIST), and VAN acknowledges postdoctoral fellowships from Alberta Innovates-Health Solutions (AIHS, 2015-2017), Canadian Institutes of Health Research (CIHR, 2016-2018), and Los Alamos National Lab Director’s Fellowship (2018-2021) for the work on VDAC. MQM, SMB, and TKR were supported by the Intramural Research Program of the National Institutes of Health, Eunice Kennedy Shriver National Institute of Child Health and Human Development. MQM acknowledges postdoctoral fellowship Juan de la Cierva Incorporación from the Spanish Ministry of Science and Innovation MCIN/AEI/10.13039/501100011033 (IJC2018-035283-I, 2020-2022) and support from Universitat Jaume I (project UJI-A2020-21). Anton 2 computer time was provided through allocation PSCA16049P to DPH and SYN by the Pittsburgh Supercomputing Center (PSC) through Grant R01GM116961 from the National Institutes of Health.
Subjects: MD simulations, General Chemistry, Molecular Dynamics Simulation, Biochemistry, Catalysis, Membrane Potentials, Mitochondria, [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, Brownian Dynamics, Colloid and Surface Chemistry, Voltage gating, Mitochondrial Membranes, Voltage-Dependent Anion Channels, Single-channel electrophysiology, X-ray crystallography
Abstract: International audience; The voltage-dependent anion channel (VDAC) is a βbarrel channel of the mitochondrial outer membrane (MOM) that passively transports ions, metabolites, polypeptides, and single-stranded DNA. VDAC responds to a transmembrane potential by "gating," i.e. transitioning to one of a variety of low-conducting states of unknown structure. The gated state results in nearly complete suppression of multivalent mitochondrial metabolite (such as ATP and ADP) transport, while enhancing calcium transport. Voltage gating is a universal property of β-barrel channels, but VDAC gating is anomalously sensitive to transmembrane potential. Here, we show that a single residue in the pore interior, K12, is responsible for most of VDAC's voltage sensitivity. Using the analysis of over 40 μs of atomistic molecular dynamics (MD) simulations, we explore correlations between motions of charged residues inside the VDAC pore and geometric deformations of the β-barrel. Residue K12 is bistable; its motions between two widely separated positions along the pore axis enhance the fluctuations of the β-barrel and augment the likelihood of gating. Single channel electrophysiology of various K12 mutants reveals a dramatic reduction of the voltage-induced gating transitions. The crystal structure of the K12E mutant at a resolution of 2.6 Å indicates a similar architecture of the K12E mutant to the wild type; however, 60 μs of atomistic MD simulations using the K12E mutant show restricted motion of residue 12, due to enhanced connectivity with neighboring residues, and diminished amplitude of barrel motions. We conclude that β-barrel fluctuations, governed particularly by residue K12, drive VDAC gating transitions.
Published: 2022
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81. Long-term air pollution exposure, greenspace and health-related quality of life in the ECRHS study

Author: Anne, Boudier, Iana, Markevych, Bénédicte, Jacquemin, Michael J, Abramson, Simone, Accordini, Bertil, Forsberg, Elaine, Fuertes, Judith, Garcia-Aymerich, Joachim, Heinrich, Ane, Johannessen, Bénédicte, Leynaert, Isabelle, Pin, Valérie, Siroux, Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), CHU Grenoble, Uniwersytet Jagielloński w Krakowie = Jagiellonian University (UJ), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Monash university, Università degli studi di Verona = University of Verona (UNIVR), Umeå University, Imperial College London, Instituto de Salud Global - Institute For Global Health [Barcelona] (ISGlobal), Universitat Pompeu Fabra [Barcelona] (UPF), Ludwig-Maximilians University [Munich] (LMU), University of Bergen (UiB), Centre de recherche en épidémiologie et santé des populations (CESP), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, Centre Hospitalier Universitaire [Grenoble] (CHU), The present analyses are part of the NEVADA project funded by the French Direction Générale de l'Offre de Soin (DGOS), call PHRC-2012.The local investigators and funding agencies for the European Community Respiratory Health Survey (ECRHS I, ECRHS II and ECRHS III) are reported in the online supplementary file.Coordination of the ECRHS I was supported by the European Commission as part of the 'Quality of Life' program and the authors and participants are grateful to the late C. Baya and M. Hallen for their help during the study and K. Vuylsteek and the members of the COMAC for their support. Coordination of the ECRHS II was supported by the European Commission as part of the 'Quality of Life' program (Grant: QLK4-CT-1999-01237). The coordination of the ECRHS 3 was funded through the Medical Research Council (Grant: 92091). NDVI calculations were conducted within the framework of the Ageing Lungs In European Cohorts study that was funded by the European Union's Horizon 2020 research and innovation program under (Grant: 633212). Iana Markevych is currently supported from the 'NeuroSmog: Determining the impact of air pollution on the developing brain' (Nr. POIR.04.04.00-1763/18-00) which is implemented as part of the TEAM-NET programme of the Foundation for Polish Science, co-financed from EU resources, obtained from the European Regional Development Fund under the Smart Growth Operational Programme. ISGlobal acknowledges support from the Spanish Ministry of Science and Innovation through the 'Centro de Excelencia Severo Ochoa 2019–2023' Program (CEX2018-000806-S), and the Generalitat de Catalunya through the CERCA Program., and Jonchère, Laurent
Subjects: Adult, Male, Environmental Engineering, greenness, PM, Epidemiology, Parks, Recreational, [SDV]Life Sciences [q-bio], Nitrogen Dioxide, Well-being, NO(2), NO2, rhinitis, well-being, Air Pollution, Humans, Environmental Chemistry, European Union, Waste Management and Disposal, Rhinitis, Air Pollutants, asthma, greenness, NO2, PM, well-being, Environmental Exposure, asthma, Health Surveys, Pollution, Asthma, [SDV] Life Sciences [q-bio], Greenness, Quality of Life, Female, Particulate Matter, epidemiology
Abstract: International audience; BACKGROUND: Associations of long-term exposure to air pollution and greenspace with health-related quality of life (HRQOL) are poorly studied and few studies have accounted for asthma-rhinitis status. OBJECTIVE: To assess the associations of air pollution and greenspace with HRQOL and whether asthma and/or rhinitis modify these associations. METHODS: The study was based on the participants in the second (2000-2002, n = 6542) and third (2011-2013, n = 3686) waves of the European Community Respiratory Health Survey (ECRHS) including 19 centres. The mean follow-up time was 11.3 years. HRQOL was assessed by the SF-36 Physical and Mental Component Summary scores (PCS and MCS). NO(2), PM(2.5) and PM(10) annual concentrations were estimated at the residential address from existing land-use regression models. Greenspace around the residential address was estimated by the (i) mean of the Normalized Difference Vegetation Index (NDVI) and by the (ii) presence of green spaces within a 300 m buffer. Associations of each exposure variable with PCS and MCS were assessed by mixed linear regression models, accounting for the multicentre design and repeated data, and adjusting for potential confounders. Analyses were stratified by asthma-rhinitis status. RESULTS: The mean (SD) age of the ECRHS-II and III participants was 43 (7.1) and 54 (7.2) years, respectively, and 48 % were men. Higher NO(2), PM(2.5) and PM(10) concentrations were associated with lower MCS (regression coefficients [95%CI] for one unit increase in the inter-quartile range of exposures were -0.69 [-1.23; -0.15], -1.79 [-2.88; -0.70], -1.80 [-2.98; -0.62] respectively). Higher NDVI and presence of forests were associated with higher MCS. No consistent associations were observed for PCS. Similar association patterns were observed regardless of asthma-rhinitis status. CONCLUSION: European adults who resided at places with higher air pollution and lower greenspace were more likely to have lower mental component of HRQOL. Asthma or rhinitis status did not modify these associations.
Published: 2022
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82. Role of the paclitaxel-eluting stent and tirofiban in patients with ST-elevation myocardial infarction undergoing postfibrinolysis angioplasty: the GRACIA-3 randomized clinical trial

Author: Francisco Fernández-Avilés, Juan Sanchis, Francisco Bosa, Pedro L. Sánchez, J. Alberto San Román, J H Alonso-Briales, Armando Bethencourt, Pablo Ancillo, Juan J Sanz, Joaquín Alonso, Juan López-Messa, Federico Gimeno, Ignacio Amat Santos, Armando Pérez de Prado, The GRACIA-3 (Grupo de Análisis de la Cardiopatía Isquémica Aguda), [Sánchez,PL, Fernández-Avilés,F] Hospital General Universitario Gregorio Marañón, Madrid, Spain. [Gimeno,F, San Román,JA] Hospital Clínico de Valladolid, Spain. [Ancillo,P] Hospital General de Segovia, Spain. [Sanz,JJ] Hospital Río Hortega, Valladolid, Spain. [Alonso Briales,JH] Hospital Universitario Virgen de la Victoria, Málaga. Spain. [Bosa,F] Hospital Universitario de Canarias, Santa Cruz de Tenerife, Spain. [Santos,I] Hospital Universitario de Salamanca, Spain. [Sanchís,J] Hospital Clínico Universitario, Valencia, Spain. [Bethencourt,A] Hospital Son Dureta, Palma de Mallorca, Spain. [López-Messa,J] Hospital Río Carrión, Palencia, Spain. [Pérez de Prado,A] Complejo Hospitalario de León, Spain. [Alonso,JJ] Hospital de Fuenlabrada, Madrid, Spain., and Unrestricted grants to fund the study were obtained from Red Temática de Enfermedades Cardiovasculares (RECAVA) of the Instituto de Salud Carlos III (Spanish Ministry of Science and Innovation), from the Fondo de Investigación Sanitaria (FIS: PI040308, PI040235, PI040361, PI042035, PI042686, PI040166, PI040276, PI 040306, PI040309, PI040350, PI040478, and PI0402037) of the Instituto de Salud Carlos III (Spanish Ministry of Science and Innovation), and from the Junta de Castilla y León.
Subjects: Male, tirofiban, Angioplastia, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Equipment and Supplies::Prostheses and Implants::Stents::Drug-Eluting Stents [Medical Subject Headings], medicine.medical_treatment, Diseases::Cardiovascular Diseases::Heart Diseases::Myocardial Ischemia::Myocardial Infarction [Medical Subject Headings], Myocardial Infarction, Infarto de Miocardio, Named Groups::Persons::Age Groups::Adult::Middle Aged [Medical Subject Headings], Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings], Electrocardiography, Mediana Edad, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Catheterization::Angioplasty [Medical Subject Headings], Myocardial infarction, Masculino, Cardiac catheterization, Femenino, Drug-Eluting Stents, Tirofiban, Middle Aged, Stents Liberadores de Fármacos, Humanos, Treatment Outcome, Cardiology, Female, Cardiology and Cardiovascular Medicine, medicine.drug, Chemicals and Drugs::Organic Chemicals::Hydrocarbons::Hydrocarbons, Cyclic::Hydrocarbons, Alicyclic::Cycloparaffins::Cyclodecanes::Taxoids::Paclitaxel [Medical Subject Headings], medicine.medical_specialty, Paclitaxel, Tenecteplase, Check Tags::Male [Medical Subject Headings], Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Electrodiagnosis::Electrocardiography [Medical Subject Headings], Internal medicine, Angioplasty, Multicenter trial, Fibrinolysis, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cohort Studies::Longitudinal Studies::Follow-Up Studies [Medical Subject Headings], medicine, Humans, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Prognosis::Treatment Outcome [Medical Subject Headings], business.industry, Stent, Electrocardiografía, medicine.disease, Surgery, Check Tags::Female [Medical Subject Headings], Estudios de Seguimiento, Tirosina, Tyrosine, Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Amino Acids::Amino Acids, Cyclic::Amino Acids, Aromatic::Tyrosine [Medical Subject Headings], business, Follow-Up Studies
Abstract: Background— A catheter-based approach after fibrinolysis is recommended if fibrinolysis is likely to be successful in patients with acute ST-elevation myocardial infarction. We designed a 2×2 randomized, open-label, multicenter trial to evaluate the efficacy and safety of the paclitaxel-eluting stent and tirofiban administered after fibrinolysis but before catheterization to optimize the results of this reperfusion strategy. Methods and Results— We randomly assigned 436 patients with acute ST-elevation myocardial infarction to (1) bare-metal stent without tirofiban, (2) bare-metal stent with tirofiban, (3) paclitaxel-eluting stent without tirofiban, and (4) paclitaxel-eluting stent with tirofiban. All patients were initially treated with tenecteplase and enoxaparin. Tirofiban was started 120 minutes after tenecteplase in those patients randomly assigned to tirofiban. Cardiac catheterization was performed within the first 3 to 12 hours after inclusion, and stenting (randomized paclitaxel or bare stent) was applied to the culprit artery. The primary objectives were the rate of in-segment binary restenosis of paclitaxel-eluting stent compared with that of bare-metal stent and the effect of tirofiban on epicardial and myocardial flow before and after mechanical revascularization. At 12 months, in-segment binary restenosis was similar between paclitaxel-eluting stent and bare-metal stent (10.1% versus 11.3%; relative risk, 1.06; 95% confidence interval, 0.74 to 1.52; P =0.89). However, late lumen loss (0.04±0.055 mm versus 0.27±0.057 mm, P =0.003) was reduced in the paclitaxel-eluting stent group. No evidence was found of any association between the use of tirofiban and any improvement in the epicardial and myocardial perfusion. Major bleeding was observed in 6.1% of patients receiving tirofiban and in 2.7% of patients not receiving it (relative risk, 2.22; 95% confidence interval, 0.86 to 5.73; P =0.14). Conclusions— This trial does not provide evidence to support the use of tirofiban after fibrinolysis to improve epicardial and myocardial perfusion. Compared with bare-metal stent, paclitaxel-eluting stent significantly reduced late loss but appeared not to reduce in-segment binary restenosis. Clinical Trial Registration— URL: http://clinicaltrials.gov . Unique identifier: NCT00306228.
Published: 2010

83. Single centrosome manipulation reveals its electric charge and associated dynamic structure

Author: Hormeño S., Ibarra B., Chichón F.J., Habermann K., Lange B.M.H., Valpuesta J.M., Carrascosa J.L., Arias-Gonzalez J.R. and This work was supported by grants from the Spanish Ministry of Science and Innovation (BFU2005-06487, CSD2006-00023, BFU2007-62382/BMC, and BFU2008-02328/BMC) and the Comunidad de Madrid (S-0505/MAT/0283). S.H. received a scholarship from Consejería de Educación de la Comunidad de Madrid and the European Social Fund. J.R.A.-G. was the recipient of an individual fellowship from the International Human Frontier Science Program Organization and a Ramón y Cajal contract from the Spanish Ministry of Science and Innovation.
Published: 2009

84. Analysis of the Influence of Power System Diversion on the Optimal Supply Strategy of Renewable Power Plants

Author: Endemaño Ventura, Lazaro, Serrano González, Javier, Riquelme Santos, Jesús Manuel, Roldan-Fernandez, Juan Manuel, CERVERA research programme, Industrial and Technological Development Centre of Spain, and Spanish Ministry of Science and Innovation
Subjects: Balancing services, electricity markets, optimal energy bidding, renewable energy
Abstract: This paper presents an analysis of the influence of power system deviation prediction on the bidding strategy of renewable generation units in spot markets. The optimal bid that a renewable generator makes is subject to the best possible forecast at the time it submits the energy bid to the spot market, which is usually between 12–36 hours in advance of the time of delivery. With these lead times, renewable generators have to assume a significant volume risk in relation to the difference that may occur between the energy finally delivered and the energy previously committed for their participation in the market, since deviations from the committed energy will be valued at the deviations price. In this sense, the analysis carried out in this work shows that the prices of deviations are highly influenced by the energy needs to be raised or lowered by the system at the time of delivery. In other words, in the event that the deviation of the renewable generator goes against the system, the generator will generally have a higher penalty, having to assume the cost of the energy deviation at a price higher than the spot market price. On the other hand, if the plant’s deviation benefits the system, the penalty will be significantly lower (and sometimes even zero). The proposed analysis methodology develops the formulation of the expected benefit of the plant obtained through its participation in the spot market and subsequent settlement of the deviations. This formulation includes the modeling of the effect of the system deviation on the plant’s profits, which allows to satisfactorily identify the influence of the prediction of this variable on the optimal offer strategy. This methodology has been tested for the case of a wind farm operating in the Spanish market. For this purpose, real data of forecasts and final production of the wind farm have been used, as well as real data of the spot market, prices of the balancing service and real deviation of the system, which has allowed to verify in totally realistic conditions the importance of the prediction of the direction of deviation of the system in the optimal bidding. In this way, it will be possible to establish new optimal bidding strategies that focus efforts on advanced prediction techniques for this variable, which will result in greater benefits for wind power plants for their participation in the energy markets.
Published: 2023
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85. A Single Hydrogen Bond Controls the Selectivity of Transglycosylation vs Hydrolysis in Family 13 Glycoside Hydrolases

Author: Zhiyong Guo, Lei Wang, Lingqia Su, Sheng Chen, Wei Xia, Isabelle André, Carme Rovira, Binju Wang, Jing Wu, Yangzhou University, Shanghai Jiao Tong University School of Medicine, Toulouse Biotechnology Institute (TBI), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Universitat de Barcelona and the Intitut de Recerca en Química Teòrica i Computacional (IQTCUB), Institució Catalana de Recerca i Estudis Avançats (ICREA), Centre Interdisciplinaire de Nanoscience de Marseille (CINaM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Jiangnan University, Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de Química Teòrica i Computacional (IQTCUB), Universitat de Barcelona (UB), Xiamen University, National Natural Science Foundation of China (NSFC, Grants 31730067 and 21933009), National Science Fund for Distinguished Young Scholars (Grant 31425020), NSFC (Grants 22073077, 32001637, and 31972032), Spanish Ministry of Science and Innovation (Grants PID2020-118893GB-I00 and MDM-2017-0767), European Research Council (Grant ERC-2020-SyG-95123), National First-Class Discipline Program of Light Industry Technology and Engineering (Grant LITE2018-03), and Postgraduate Research & Practice Innovation Program of Jiangsu Province (Grant KYCX19_1839).
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Glycosylation, Glycoside Hydrolases, Hydrolysis, General Materials Science, Hydrogen Bonding, [CHIM.CATA]Chemical Sciences/Catalysis, Physical and Theoretical Chemistry, Hydrogen
Abstract: International audience; Converting glycoside hydrolases (GHs) from hydrolytic to synthetic enzymes via transglycosylation is a long-standing goal for the biosynthesis of complex carbohydrates. However, the molecular determinants for the selectivity of transglycosylation (T) vs hydrolysis (H) are still not fully unraveled. Herein, we show experimentally that mutation of one active site residue can switch the enzyme activity between hydrolysis and transglycosylation in two highly homologous GHs. Further QM/MM simulations reveal that the mutation modulates the T vs H reaction barriers via the presence/absence of a single H-bond with the nucleophile Asp. Such a H-bond controls the product selectivity via a dual effect: on one hand, it facilitates the breaking of the glycosyl-enzyme intermediate. On the other, it displaces the sugar acceptor, resulting in a reduced affinity and significant steric repulsion for transglycosylation. These findings expand our understanding of the molecular mechanisms that modulate the T/H balance in GHs.
Published: 2022
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86. Revisiting Vibrational Spectroscopy to Tackle the Chemistry of Zr₆O₈ Metal-Organic Framework Nodes

Author: Romero-Muñiz, Ignacio, Romero-Muñiz, Carlos, Castillo-Velilla, Isabel del, Marini, Carlo, Calero, Sofía, Zamora Abanades, Félix, Platero-Prats, Ana E., Universidad de Sevilla. Departamento de Física Aplicada I, MCIN/AEI/10.13039/501100011033 and EUR2020-112294 RTI2018-096138-A-I00, and Spanish Ministry of Science and Innovation through the 'María de Maeztu' Programme for Units of Excellence in R&D CEX2018-000805-M
Subjects: Vibrational spectroscopy, Local structure, 1,3-dipolar cycloaddition, Density functional theory calculations, Zirconium metal-organic frameworks
Abstract: The metal-organic framework MOF-808 contains Zr₆O₈ nodes with a high density of vacancy sites, which can incorporate carboxylate-containing functional groups to tune chemical reactivity. Although the postsynthetic methods to modify the chemistry of the Zr₆O₈ nodes in MOFs are well known, tackling these alterations from a structural perspective is still a challenge. We have combined infrared spectroscopy experiments and first-principles calculations to identify the presence of node vacancies accessible for chemical modifications within the MOF-808. We demonstrate the potential of our approach to assess the decoration of MOF-808 nodes with different catechol–benzoate ligands. Furthermore, we have applied advanced synchrotron characterization tools, such as pair distribution function analyses and X-ray absorption spectroscopy, to resolve the atomic structure of single metal sites incorporated into the catechol groups postsynthetically. Finally, we demonstrate the catalytic activity of these MOF-808 materials decorated with single copper sites for 1,3-dipolar cycloadditions.
Published: 2022

87. Rhodium-based cathodes with ultra-low metal loading to increase the sustainability in the hydrogen evolution reaction

Author: Gema Pérez, Guillermo Díaz-Sainz, Lucía Gómez-Coma, Lucía Álvarez-Miguel, Aymeric Garnier, Nolwenn Cabon, Alfredo Ortiz, Frederic Gloaguen, Inmaculada Ortiz, Universidad de Cantabria [Santander], Chimie, Electrochimie Moléculaires et Chimie Analytique (CEMCA), Université de Brest (UBO)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO), Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), The authors of this work would like to show their gratitude to the financial support from the European Regional Development Fund in the framework of the Interreg Atlantic program through the project 'HYLANTIC'-EAPA_204/2016'. The Spanish Ministry of Science and Innovation has also supported this work through the project PLEC2021-007718. Besides, we thank M.-L. Rouget (Pôle Spectrométrie Ocean, IUEM Brest) and Dr. M. Waeles (LEMAR, UBO Brest) for their help in elemental ICP-MS analysis, Dr. S. Rioual (Lab-STICC, UBO Brest) for his help in XPS analysis, and P. Elies (SC PIMM, UBO Brest) for his help in SEM-EDX analysis. The authors thank for technical and human support provided by SGIker of UPV/EHU and European funding (ERDF and ESF)., and Universidad de Cantabria
Subjects: Low-metal loading, Process Chemistry and Technology, Chemical Engineering (miscellaneous), HER, PEM Electrolyzer, Organometallic catalysis, Rh-based electrocatalysts, [CHIM.CATA]Chemical Sciences/Catalysis, Pollution, Waste Management and Disposal, Hydrogen evolution reaction
Abstract: Climate change mitigation is one of the main global challenges in the 21st century. In this context, the recent 26th United Nations Climate Change Conference of the Parties (COP26-Glasgow) claimed for searching urgent and efficient measures to reduce and ultimately avoid CO2 emissions. Thus, many efforts from the scientific community focus on the research of new and renewable energy sources (RES). Among other approaches, green hydrogen, which comes from water electrolysis, is a promising candidate to be considered in the energy panorama. However, commercial electrolyzers are provided with Pt/C and Ir-based electrocatalytic materials, which are expensive and not abundant, to catalyze the Hydrogen Evolution Reaction (HER) in safe, stable, inexpensive, and environmentaly friendly conditions. Thus, this work aims to synthesize high-performance and very low metal loading catalysts by immobilizing a Rh-based organometallic complex (RhCp*Cl(phendiamine)]Cl) on a carbon black support following a robust synthesis procedure. Advanced characterization of the synthesized materials confirmed that ultra-low metal loadings in the range of 3.2-4.7 mg·g-1 were successfully reached. Subsequently, Rh-based catalysts were tested in a PEM electrolyzer. For metal loadings as low as 0.0066 mg·cm-2 competitive cell potentials of 1.9V were achieved working with 0.5A·cm-2 geometric current density at 70°C. These results are comparable to those obtained with Pt-based commercial cathodes working under similar operation conditions. Thus, the results of this research make a step forward in the substitution of conventional cathodes for the electrolytic HER by new materials with very low metal loadings. The authors of this work would like to show their gratitude to the financial support from the European Regional Development Fund in the framework of the Interreg Atlantic program through the project “HYLANTIC”-EAPA_204/2016”. The Spanish Ministry of Science and Innovation has also supported this work through the project PLEC2021-007718.
Published: 2022
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88. Evolution of plasmid mobility: origin and fate of conjugative and nonconjugative plasmids

Author: Charles Coluzzi, Maria Pilar Garcillán-Barcia, Fernando de la Cruz, Eduardo P.C. Rocha, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Universidad de Cantabria, Génomique évolutive des Microbes / Microbial Evolutionary Genomics, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Instituto de Biomedicina y Biotecnología de Cantabria [Santander, Espagne] (IBBTEC), Universidad de Cantabria [Santander], INCEPTION project (PIA/ANR-16-CONV-0005), Equipe FRM (EQU201903007835), Laboratoire d’Excellence IBEID (ANR-10-LABX-62-IBEID) to E.P.C.R. and C.C. PID2020-117923GB-I00 project funded by Spanish Ministry of Science and Innovation to FdlC and MPG-B., ANR-16-CONV-0005,INCEPTION,Institut Convergences pour l'étude de l'Emergence des Pathologies au Travers des Individus et des populatiONs(2016), and ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010)
Subjects: [SDV.GEN.GPO]Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE], Gene Transfer, Horizontal, Bacteria, Conjugation, [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE], Horizontal gene transfer, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], Prokaryotic Cells, Conjugation, Genetic, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Genetics, DNA Transposable Elements, [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM], Genomes, Molecular Biology, Ecology, Evolution, Behavior and Systematics, Plasmids
Abstract: Conjugation drives the horizontal transfer of adaptive traits across prokaryotes. One-fourth of the plasmids encode the functions necessary to conjugate autonomously, the others being eventually mobilizable by conjugation. To understand the evolution of plasmid mobility, we studied plasmid size, gene repertoires, and conjugation-related genes. Plasmid gene repertoires were found to vary rapidly in relation to the evolutionary rate of relaxases, for example, most pairs of plasmids with 95% identical relaxases have fewer than 50% of homologs. Among 249 recent transitions of mobility type, we observed a clear excess of plasmids losing the capacity to conjugate. These transitions are associated with even greater changes in gene repertoires, possibly mediated by transposable elements, including pseudogenization of the conjugation locus, exchange of replicases reducing the problem of incompatibility, and extensive loss of other genes. At the microevolutionary scale of plasmid taxonomy, transitions of mobility type sometimes result in the creation of novel taxonomic units. Interestingly, most transitions from conjugative to mobilizable plasmids seem to be lost in the long term. This suggests a source-sink dynamic, where conjugative plasmids generate nonconjugative plasmids that tend to be poorly adapted and are frequently lost. Still, in some cases, these relaxases seem to have evolved to become efficient at plasmid mobilization in trans, possibly by hijacking multiple conjugative systems. This resulted in specialized relaxases of mobilizable plasmids. In conclusion, the evolution of plasmid mobility is frequent, shapes the patterns of gene flow in bacteria, the dynamics of gene repertoires, and the ecology of plasmids., INCEPTION project (PIA/ANR-16-CONV-0005), Equipe FRM (EQU201903007835), Laboratoire d’Excellence IBEID (ANR-10-LABX-62-IBEID) to E.P.C.R. and C.C. PID2020-117923GB-I00 project funded by Spanish Ministry of Science and Innovation to FdlC and MPG-B.
Published: 2022
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89. Listeria valentina sp. nov., isolated from a water trough and the faeces of healthy sheep

Author: Alexandra Moura, Ángel Gómez-Martín, Guillaume Vales, Nathalie Tessaud-Rita, Ángel García-Muñoz, Marc Lecuit, Juan J. Quereda, Alexandre Leclercq, Hélène Bracq-Dieye, Pierre Thouvenot, Universidad Cardenal Herrera-CEU (CEU-UCH), Biologie des Infections - Biology of Infection, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de Référence Listeria - National Reference Center Listeria (CNRL), Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria (CC-OMS / WHO-CC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Service des Maladies infectieuses et tropicales [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), This work was supported by Generalitat Valenciana (Project reference GV/2018/A/183) and by the Spanish Ministry of Science and Innovation (Project reference PID2019-110764RA-I00), Institut Pasteur, Inserm and Santé Publique France. J.J. Quereda is supported by a ‘Ramón y Cajal’ contract of the Spanish Ministry of Science, Innovation and Universities (RYC-2018–024985-I)., DIAKITE, andrée, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), and Institut Pasteur [Paris]-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO)
Subjects: POCP, [SDV]Life Sciences [q-bio], Context (language use), Microbiology, new taxa, 03 medical and health sciences, Monophyly, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Clade, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, isDDH, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, Whole genome sequencing, whole genome sequencing, 0303 health sciences, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, biology, Phylogenetic tree, AAI, 030306 microbiology, General Medicine, isDDH Subject category: International Journal of Systematic and Evolutionary Microbiology Designation of Neotype Strains Subject: New Taxa -Firmicutes and Related Organisms, 16S ribosomal RNA, biology.organism_classification, [SDV] Life Sciences [q-bio], [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Taxon, ANI, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Listeria, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: In the context of a study on the occurrence of Listeria species in an animal farm environment in Valencia, Spain, six Listeria -like isolates could not be assigned to any known species. Phylogenetic analysis based on the 16S rRNA gene and on 231 Listeria core genes grouped these isolates in a monophyletic clade within the genus Listeria , with highest similarity to Listeria thailandensis . Whole-genome sequence analyses based on in silico DNA–DNA hybridization, the average nucleotide blast and the pairwise amino acid identities against all currently known Listeria species confirmed that these isolates constituted a new taxon within the genus Listeria . Phenotypically, these isolates differed from other Listeria species mainly by the production of acid from inositol, the absence of acidification in presence of methyl α-d-glucoside, and the absence of α-mannosidase and nitrate reductase activities. The name Listeria valentina sp. nov. is proposed for this novel species, and the type strain is CLIP 2019/00642T (=CIP 111799T=DSM 110544T).
Published: 2020
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90. The Mycobacterium tuberculosis PhoPR virulence system regulates expression of the universal second messenger c-di-AMP and impacts vaccine safety and efficacy

Author: Irene Pérez, Elena Campos-Pardos, Caridad Díaz, Santiago Uranga, Fadel Sayes, Francisca Vicente, Nacho Aguiló, Roland Brosch, Carlos Martín, Jesús Gonzalo-Asensio, University of Zaragoza - Universidad de Zaragoza [Zaragoza], Instituto de Salud Carlos III [Madrid] (ISC), Fundación MEDINA [Granada], Pathogénomique mycobactérienne intégrée - Integrated Mycobacterial Pathogenomics, Université Paris Cité (UPCité)-Microbiologie Intégrative et Moléculaire (UMR6047), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Hospital Universitario Miguel Servet, Instituto de Biocomputación y Física de Sistemas Complejos = Institute for Biocomputation and Physics of Complex Systems (BIFI), and This work was supported by a grant from the Spanish Ministry of Science and Innovation to J.G.-A. (reference PID2019-104690RB-I00). I.P. was a recipient of a 'DGA-Fondo Social Europeo' grant. I.P. also was a recipient of a 'Programa CAI-Ibercaja Estancias de Investigación' grant (CM3/18) and a 'FEMS Research and Training' grant (FEMS-GO-2018-118) to perform an internship at the Integrated Mycobacterial Pathogenomics Unit at the Institut Pasteur of Paris. MEDINA authors disclosed the receipt of financial support from Fundación MEDINA, a public–private partnership of Merck Sharp & Dohme de España S.A./Universidad de Granada/Junta de Andalucía (PIN-0474-2016).
Subjects: MT:therapies and applications, live vaccines, IFN, tuberculosis, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], Drug Discovery, PhoPR, Molecular Medicine, BCG, therapies and applications [MT], [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], MTBVAC, innate immunity, cyclic dinucleotides
Abstract: International audience; Cyclic (di)nucleotides act as universal second messengers endogenously produced by several pathogens. Specifically, the roles of c-di-AMP in Mycobacterium tuberculosis immunity and virulence have been largely explored, although its contribution to the safety and efficacy of live tuberculosis vaccines is less understood. In this study, we demonstrate that the synthesis of c-di-AMP is negatively regulated by the M. tuberculosis PhoPR virulence system. Accordingly, the live attenuated tuberculosis vaccine candidate M. tuberculosis vaccine (MTBVAC), based on double phoP and fadD26 deletions, produces more than 25- and 45-fold c-di-AMP levels relative to wild-type M. tuberculosis or the current vaccine bacille Calmette-Guérin (BCG), respectively. Secretion of this second messenger was exclusively detected in MTBVAC but not in M. tuberculosis or in BCG. We also demonstrate that c-di-AMP synthesis during in vitro cultivation of M. tuberculosis is a growth-phase- and medium-dependent phenotype. To uncover the role of this metabolite in the vaccine properties of MTBVAC, we constructed and validated knockout and overproducing/oversecreting derivatives by inactivating the disA or cnpB gene, respectively. All MTBVAC derivatives elicited superior interleukin-1β (IL-1β) responses compared with BCG during an in vitro infection of human macrophages. However, both vaccines failed to elicit interferon β (IFNβ) activation in this cellular model. We found that increasing c-di-AMP levels remarkably correlated with a safer profile of tuberculosis vaccines in the immunodeficient mouse model. Finally, we demonstrate that overproduction of c-di-AMP due to cnpB inactivation resulted in lower protection of MTBVAC, while the absence of c-di-AMP in the MTBVAC disA derivative maintains the protective efficacy of this vaccine in mice.
Published: 2022
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91. Systemic Inflammatory Biomarkers Define Specific Clusters in Patients with Bronchiectasis : A Large-Cohort Study

Author: Xuejie Wang, Carmen Villa, Yadira Dobarganes, Casilda Olveira, Rosa Girón, Marta García-Clemente, Luis Máiz, Oriol Sibila, Rafael Golpe, Rosario Menéndez, Juan Rodríguez-López, Concepción Prados, Miguel Angel Martinez-García, Juan Luis Rodriguez, David de la Rosa, Xavier Duran, Jordi Garcia-Ojalvo, Esther Barreiro, [Wang, Xuejie] Hosp Mar IMIM, Pulmonol Dept, Lung Canc & Muscle Res Grp, PRBB, Parc Salut Mar,C Dr Aiguader 88, Barcelona 08003, Spain, [Barreiro, Esther] Hosp Mar IMIM, Pulmonol Dept, Lung Canc & Muscle Res Grp, PRBB, Parc Salut Mar,C Dr Aiguader 88, Barcelona 08003, Spain, [Wang, Xuejie] Univ Autonoma Barcelona UAB, Dept Med, Barcelona 08035, Spain, [Villa, Carmen] Clin Fuensanta, Resp Dept, Madrid 28015, Spain, [Dobarganes, Yadira] Clin Fuensanta, Resp Dept, Madrid 28015, Spain, [Olveira, Casilda] Hosp Reg Univ Malaga, Resp Dept, Malaga 29003, Spain, [Olveira, Casilda] Univ Malaga, Inst Invest Biomed Malaga IBIMA, Malaga 29003, Spain, [Giron, Rosa] Hosp Univ la Princesa, Inst Invest Sanitaria, Resp Dept, Madrid 28015, Spain, [Garcia-Clemente, Marta] Hosp Univ Cent Asturias, Resp Dept, Oviedo 33071, Spain, [Maiz, Luis] Hosp Ramon & Cajal, Resp Dept, Madrid 28015, Spain, [Sibila, Oriol] Hosp Clin Barcelona, Resp Dept, Barcelona 08035, Spain, [Sibila, Oriol] Inst Salud Carlos III ISCIII, Ctr Invest Red Enfermedades Resp CIBERES, Madrid 28015, Spain, [Angel Martinez-Garcia, Miguel] Inst Salud Carlos III ISCIII, Ctr Invest Red Enfermedades Resp CIBERES, Madrid 28015, Spain, [Barreiro, Esther] Inst Salud Carlos III ISCIII, Ctr Invest Red Enfermedades Resp CIBERES, Madrid 28015, Spain, [Golpe, Rafael] Hosp Lucus Augusti, Resp Dept, Lugo 27080, Spain, [Menendez, Rosario] Hosp Univ & Politecn La Fe, Resp Dept, Valencia 46003, Spain, [Angel Martinez-Garcia, Miguel] Hosp Univ & Politecn La Fe, Resp Dept, Valencia 46003, Spain, [Rodriguez-Lopez, Juan] Hosp San Agustin, Resp Dept, Aviles 33401, Spain, [Prados, Concepcion] Hosp La Paz, Resp Dept, Madrid 28015, Spain, [Luis Rodriguez, Juan] Hosp Clin San Carlos, Resp Dept, Madrid 28015, Spain, [Luis Rodriguez, Juan] Inst Invest Sanitaria Hosp Clin San Carlos IdISSC, Madrid 28015, Spain, [Luis Rodriguez, Juan] Univ Complutense Madrid, Dept Med, Madrid 28015, Spain, [de la Rosa, David] Hosp Santa Creu & Sant Pau, Resp Dept, Barcelona 08035, Spain, [Duran, Xavier] Hosp Mar IMIM, Sci & Tech Dept, Barcelona 08035, Spain, [Garcia-Ojalvo, Jordi] Univ Pompeu Fabra UPF, Dept Hlth & Expt Sci CEXS, Barcelona 08035, Spain, [Barreiro, Esther] Univ Pompeu Fabra UPF, Dept Hlth & Expt Sci CEXS, Barcelona 08035, Spain, FEDER, ISC-III, CIBERES (ISC-III), SEPAR, ZAMBON-PHARMA (Spain), Spanish Ministry of Science and Innovation, FEDER, Maria de Maeztu Programme for Units of Excellence in RD, and Generalitat de Catalunya (ICREA Academia programme)
Subjects: Non-cystic fibrosis bronchiectasis, C reactive protein, Disease severity scores, Cystic fibrosis bronchiectasis, Blood neutrophil, Medicine (miscellaneous), Guidelines, Eosinophil, General Biochemistry, Genetics and Molecular Biology, Hierarchical clustering, Multivariate analyses, Lymphocyte counts, Clinical outcomes, non-cystic fibrosis bronchiectasis, blood neutrophil, eosinophil, lymphocyte counts, hemoglobin, hierarchical clustering, phenotypic clusters, multivariate analyses, clinical outcomes, disease severity scores, Hematología, Hemoglobin, Neumología, Phenotypic clusters
Abstract: Differential phenotypic characteristics using data mining approaches were defined in a large cohort of patients from the Spanish Online Bronchiectasis Registry (RIBRON). Three differential phenotypic clusters (hierarchical clustering, scikit-learn library for Python, and agglomerative methods) according to systemic biomarkers: neutrophil, eosinophil, and lymphocyte counts, C reactive protein, and hemoglobin were obtained in a patient large-cohort (n = 1092). Clusters #1-3 were named as mild, moderate, and severe on the basis of disease severity scores. Patients in cluster #3 were significantly more severe (FEV1, age, colonization, extension, dyspnea (FACED), exacerbation (EFACED), and bronchiectasis severity index (BSI) scores) than patients in clusters #1 and #2. Exacerbation and hospitalization numbers, Charlson index, and blood inflammatory markers were significantly greater in cluster #3 than in clusters #1 and #2. Chronic colonization by Pseudomonas aeruginosa and COPD prevalence were higher in cluster # 3 than in cluster #1. Airflow limitation and diffusion capacity were reduced in cluster #3 compared to clusters #1 and #2. Multivariate ordinal logistic regression analysis further confirmed these results. Similar results were obtained after excluding COPD patients. Clustering analysis offers a powerful tool to better characterize patients with bronchiectasis. These results have clinical implications in the management of the complexity and heterogeneity of bronchiectasis patients. This study has been supported by FIS 21/00215 (FEDER, ISC-III) & CIBERES (ISC-III), SEPAR 2020, and ZAMBON-PHARMA (Spain). JGO was supported by the Spanish Ministry of Science and Innovation and FEDER (project PGC 2018-101251-B-I00 and Maria de Maeztu Programme for Units of Excellence in R&D, grant CEX2018-000792-M), and by the Generalitat de Catalunya (ICREA Academia programme).
Published: 2022

92. Application of dry olive residue-based biochar in combination with arbuscular mycorrhizal fungi enhances the microbial status of metal contaminated soils

Author: José A. Siles, Inmaculada García-Romera, Tomas Cajthaml, Jorge Belloc, Gloria Silva-Castro, Jirina Szaková, Pavel Tlustos, Mercedes Garcia-Sanchez, European Commission, Ministerio de Ciencia e Innovación (España), University of California [Berkeley] (UC Berkeley), University of California (UC), Estación Experimental del Zaidín (EEZ), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Institute of Microbiology of the Czech Academy of Sciences [Prague, Czech Republic] (MBU / CAS), Czech Academy of Sciences [Prague] (CAS), Charles University [Prague] (CU), Czech University of Life Sciences Prague (CZU), Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes (UMR Eco&Sols), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), European Regional Development Fund-Project No. CZ.02.1.01/0.0/0.0/16_019/0000845CZ.02.1.01/0.0/0.0/16_019/0000845, Center for Geosphere Dynamics (UNCE/SCI/006), Operational Programme Prague-Competitiveness (Project No. CZ.2.16/3.1.00/21516), and Spanish Ministry of Science and Innovation Project No. RTI2018-094327-B-I00.
Subjects: Soil, Multidisciplinary, Metals, [SDE.IE]Environmental Sciences/Environmental Engineering, Charcoal, Mycorrhizae, Olea, Fungi, Soil Pollutants, [SDV.SA.SDS]Life Sciences [q-bio]/Agricultural sciences/Soil study, Oxidoreductases, Plant Roots, Soil Microbiology
Abstract: Biochar made-up of dry olive residue (DOR), a biomass resulting from the olive oil extraction industry, has been proposed to be used as a reclamation agent for the recovery of metal contaminated soils. The aim of the present study was to investigate whether the soil application of DOR-based biochar alone or in combination with arbuscular mycorrhizal fungi (AMF) leads to an enhancement in the functionality and abundance of microbial communities inhabiting metal contaminated soils. To study that, a greenhouse microcosm experiment was carried out, where the effect of the factors (i) soil application of DOR-based biochar, (ii) biochar pyrolysis temperature (considering the variants 350 and 500 °C), (iii) soil application dose of biochar (2 and 5%), (iv) soil contamination level (slightly, moderately and highly polluted), (v) soil treatment time (30, 60 and 90 days) and (vi) soil inoculation with Funneliformis mosseae (AM fungus) on β-glucosidase and dehydrogenase activities, FA (fatty acid)-based abundance of soil microbial communities, soil glomalin content and AMF root colonization rates of the wheat plants growing in each microcosm were evaluated. Biochar soil amendment did not stimulate enzyme activities but increased microbial abundances. Dehydrogenase activity and microbial abundances were found to be higher in less contaminated soils and at shorter treatment times. Biochar pyrolysis temperature and application dose differently affected enzyme activities, but while the first factor did not have a significant effect on glucosidase and dehydrogenase, a higher biochar dose resulted in boosted microbial abundances. Soil inoculation with F. mosseae favored the proliferation of soil AMF community and increased soil glomalin content as well as rates of AMF root colonization. This factor also interacted with many of the others evaluated to significantly affect soil enzyme activities, microbial abundances and AMF community. Our results indicate that the application of DOR-based biochar along with AMF fungi is an appropriate approach to improve the status of microbial communities in soils with a moderate metal contamination at short-term., Authors thank for financial support of the European Regional Development Fund—Project No. CZ.02.1.01/0.0/0.0/16_019/0000845CZ.02.1.01/0.0/0.0/16_019/0000845 and by the Center for Geosphere Dynamics (UNCE/SCI/006) and part of the equipment was supported by the Operational Programme Prague—Competitiveness (Project No. CZ.2.16/3.1.00/21516). This study was also co-funded by the Spanish Ministry of Science and Innovation Project No. RTI2018-094327-B-I00.
Published: 2022
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93. Stability in Gagliardo-Nirenberg-Sobolev inequalities: flows, regularity and the entropy method

Author: Bonforte, Matteo, Dolbeault, Jean, Nazaret, Bruno, Simonov, Nikita, Instituto de Ciencias Matemàticas [Madrid] (ICMAT), Universidad Carlos III de Madrid [Madrid] (UC3M)-Universidad Complutense de Madrid = Complutense University of Madrid [Madrid] (UCM)-Universidad Autónoma de Madrid (UAM)-Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), CEntre de REcherches en MAthématiques de la DEcision (CEREMADE), Université Paris Dauphine-PSL, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Statistique, Analyse et Modélisation Multidisciplinaire (SAmos-Marin Mersenne) (SAMM), Université Paris 1 Panthéon-Sorbonne (UP1), Fédération Parisienne de Modélisation Mathématique (FP2M), Centre National de la Recherche Scientifique (CNRS), Méthodes numériques pour le problème de Monge-Kantorovich et Applications en sciences sociales (MOKAPLAN), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Dauphine-PSL, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Inria de Paris, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Project MTM2017-85757-P (Ministry of Science and Innovation, Spain) and the Spanish Ministry of Science and Innovation, through the 'Severo Ochoa Programme for Centres of Excellence in R&D' (CEX2019-000904-S)E.U. H2020 MSCA programme, grant agreement 777822Project EFI (ANR-17-CE40-0030) of the French National Research Agency (ANR)Inria Mokaplan teamDIM Math-Innov of the Region Île-de-France, ANR-17-CE40-0030,EFI,Entropie, flots, inégalités(2017), and European Project: 777822,GHAIA(2017)
Subjects: intermediate asymptotics, Mathematics::Analysis of PDEs, fast diffusion equation, rates of convergence, stability, Harnack Principle, Hardy-Poincaré inequalities, spectral gap, [MATH.MATH-AP]Mathematics [math]/Analysis of PDEs [math.AP], self-similar Barenblatt solutions, asymptotic behavior, 2020 Mathematics Subject Classification.26D10, 46E35, 35K55, 49J40, 35B40, 49K20, 49K30, 35J20, Gagliardo-Nirenberg inequality, entropy methods
Abstract: Ce document contient hal-02887010, v1: Stability in Gagliardo-Nirenberg inequalities and hal-02887013, v1: Stability in Gagliardo-Nirenberg inequalities. Supplementary material, avec plusieurs additions dont: Chapitre 1 (variational methods) et Chapitre 6 (Sobolev's inequality).; International audience; The purpose of this work is to establish a quantitative and constructive stability result for a class of subcritical Gagliardo-Nirenberg-Sobolev inequalities which interpolates between the logarithmic Sobolev inequality and the standard Sobolev inequality (in dimension larger than three), or Onofri's inequality in dimension two. We develop a new strategy, in which the flow of the fast diffusion equation is used as a tool: a stability result in the inequality is equivalent to an improved rate of convergence to equilibrium for the flow. The regularity properties of the parabolic flow allow us to connect an improved entropy - entropy production inequality during an initial time layer to spectral properties of a suitable linearized problem which is relevant for the asymptotic time layer. Altogether, the stability in the inequalities is measured by a deficit which controls in strong norms (a Fisher information which can be interpreted as a generalized Heisenberg uncertainty principle) the distance to the manifold of optimal functions. The method is constructive and, for the first time, quantitative estimates of the stability constant are obtained, including in the critical case of Sobolev's inequality. To build the estimates, we establish a quantitative global Harnack principle and perform a detailed analysis of large time asymptotics by entropy methods.
Published: 2022

94. The genetic architecture of language functional connectivity

Author: Yasmina Mekki, Vincent Guillemot, Hervé Lemaître, Amaia Carrión-Castillo, Stephanie Forkel, Vincent Frouin, Cathy Philippe, Service NEUROSPIN (NEUROSPIN), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Groupe d'imagerie neurofonctionnelle (GIN), Institut des Maladies Neurodégénératives [Bordeaux] (IMN), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Basque Center on Cognition Brain and Language [Gipuzkoa, Espagne] (BCBL), Ecole doctorale Cerveau Cognition et Comportement [Paris] (ED 158 - 3C), Sorbonne Université (SU), King‘s College London, This project was supported by the Marie Sklodowska-Curie program awarded to Stephanie J. Forkel (Grant agreement No. 101028551). Amaia Carrion-Castillo was supported by a Juan de la Cierva fellowship from the Spanish Ministry of Science and Innovation, and a Gipuzkoa Fellows fellowship from the Basque Government., and Gestionnaire, HAL Sorbonne Université 5
Subjects: Adult, Male, Resting-state functional MRI, UK Biobank, Imaging-genetics, Endophenotypes, Cognitive Neuroscience, Gene Expression, Neurosciences. Biological psychiatry. Neuropsychiatry, 03 medical and health sciences, 0302 clinical medicine, Connectome, Humans, GWAS, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Aged, Biological Specimen Banks, 030304 developmental biology, Language, Cerebral Cortex, 0303 health sciences, Psycholinguistics, Middle Aged, Magnetic Resonance Imaging, Neurology, Multivariate analysis, Female, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Nerve Net, 030217 neurology & neurosurgery, Genome-Wide Association Study, RC321-571
Abstract: Available online 18 December 2021 Language is a unique trait of the human species, of which the genetic architecture remains largely unknown. Through language disorders studies, many candidate genes were identified. However, such complex and multi- factorial trait is unlikely to be driven by only few genes and case-control studies, suffering from a lack of power, struggle to uncover significant variants. In parallel, neuroimaging has significantly contributed to the under- standing of structural and functional aspects of language in the human brain and the recent availability of large scale cohorts like UK Biobank have made possible to study language via image-derived endophenotypes in the general population. Because of its strong relationship with task-based fMRI (tbfMRI) activations and its easiness of acquisition, resting-state functional MRI (rsfMRI) have been more popularised, making it a good surrogate of functional neuronal processes. Taking advantage of such a synergistic system by aggregating effects across spa- tially distributed traits, we performed a multivariate genome-wide association study (mvGWAS) between genetic variations and resting-state functional connectivity (FC) of classical brain language areas in the inferior frontal (pars opercularis, triangularis and orbitalis), temporal and inferior parietal lobes (angular and supramarginal gyri), in 32,186 participants from UK Biobank. Twenty genomic loci were found associated with language FCs, out of which three were replicated in an independent replication sample. A locus in 3p11.1, regulating EPHA3 gene expression, is found associated with FCs of the semantic component of the language network, while a lo- cus in 15q14, regulating THBS1 gene expression is found associated with FCs of the perceptual-motor language processing, bringing novel insights into the neurobiology of language. This research was conducted using the UK Biobank resource un- der application #64984. This project was supported by the Marie Sklodowska-Curie program awarded to Stephanie J. Forkel (Grant agree- ment No. 101028551). Amaia Carrion-Castillo was supported by a Juan de la Cierva fellowship from the Spanish Ministry of Science and Innova- tion, and a Gipuzkoa Fellows fellowship from the Basque Government
Published: 2022
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95. Urban environment and cognitive and motor function in children from four European birth cohorts

Author: Marina Vafeiadi, Raquel Soler-Blasco, Mariza Kampouri, Mònica Guxens, Martine Vrijheid, Johanna Lepeule, Llúcia González-Safont, Mark J. Nieuwenhuijsen, Mark Mon-Williams, John Wright, Rosie McEachan, Anne-Claire Binter, Claire Philippat, Jonathan Y. Bernard, Jordi Sunyer, Loreto Santa-Marina, Leda Chatzi, Lucia Alonso, Ainara Andiarena, Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Instituto de Salud Global - Institute For Global Health [Barcelona] (ISGlobal), Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Agency for science, technology and research [Singapore] (A*STAR), University of South-Eastern Norway (USN), University of the Basque Country/Euskal Herriko Unibertsitatea (UPV/EHU), CIBER de Epidemiología y Salud Pública (CIBERESP), Universitat Jaume I, University of Crete [Heraklion] (UOC), Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana [Espagne] (FISABIO), University of Bradford, University of Southern California (USC), Universitat Pompeu Fabra [Barcelona] (UPF), Erasmus University Medical Center [Rotterdam] (Erasmus MC), This work was supported by funding from the European Community’s Seventh Framework Programme [FP7/2007–206 n◦308333, the HELIX project]. This INMA cohort was funded by grants from Instituto de Salud Carlos III (Red INMA G03/176, CB06/02/0041, PI041436, PI081151 incl. FEDER funds, FIS PI06/0867, FIS-PI09/00090, FIS and FIS-PI18/01142 incl. FEDER funds, FIS-FEDER: PI03/1615, PI04/1509, PI04/1112, PI04/1931, PI05/1079, PI05/1052, PI06/1213, PI07/0314, PI09/02647, PI11/01007, PI11/02591, PI11/02038, PI13/1944, PI13/2032, PI14/00891, PI14/01687, PI16/1288, PI16/00118 and PI17/00663, FIS-FSE: 17/00260, Miguel Servet-FEDER CP11/00178, CP15/00025, CPII16/00051, and CPII18/00018), from UE (FP7-ENV-2011 cod 282957, HEALTH.2010.2.4.5-1, and H2020 n◦824989), Generalitat de Catalunya-CIRIT 1999SGR 00241, Fundació La marató de TV3 (090430), Generalitat Valenciana: FISABIO (UGP 15-230, UGP-15-244, and UGP-15-249), Alicia Koplowitz Foundation 2017, CIBERESP, Department of Health of the Basque Government (2013111089, 2009111069, 2013111089, 2015111065 and 2018111086), Provincial Government of Gipuzkoa (DFG06/002, DFG08/001, DFG15/221 and DFG 89/17) and annual agreements with the municipalities of the study area (Zumarraga, Urretxu , Legazpi, Azkoitia y Azpeitia y Beasain). We acknowledge support from the Spanish Ministry of Science and Innovation and the State Research Agency through the 'Centro de Excelencia Severo Ochoa 2019-2023' Program (CEX2018-000806-S), and support from the Generalitat de Catalunya through the CERCA Program. The work was also supported by MICINN [MTM2015-68140- R] and Centro Nacional de Genotipado- CEGEN- PRB2- ISCIII (Spain). The Rhea project was financially supported by European projects, and the Greek Ministry of Health (Program of Prevention of obesity and neurodevelopmental disorders in preschool children, in Heraklion district, Crete, Greece: 2011–2014, 'Rhea Plus': Primary Prevention Program of Environmental Risk Factors for Reproductive Health, and Child Health: 2012–15). This paper presents independent research funded by the National Institute for Health Research (NIHR) under its Collaboration for Applied Health Research and Care (CLAHRC) for Yorkshire and Humber (UK). The EDEN study was supported by Foundation for medical research (FRM), National Agency for Research (ANR), National Institute for Research in Public health (IRESP: TGIR cohorte santé 2008 program), French Ministry of Health (DGS), French Ministry of Research, INSERM Bone and Joint Diseases National Research (PRO-A), and Human Nutrition National Research Programs, Paris-Sud University, Nestlé, French National Institute for Population Health Surveillance (InVS), French National Institute for Health Education (INPES), the European Union FP7 programs (ESCAPE, ENRIECO, Medall projects), Diabetes National Research Program (through a collaboration with the French Association of Diabetic Patients (AFD)), French Agency for Environmental Health Safety and French National Agency for Food Security (now ANSES), Mutuelle Générale de l’Education Nationale a complementary health insurance (MGEN), French-speaking association for the study of diabetes and metabolism (ALFEDIAM). Core support for Born in Bradford is also provided by the Wellcome Trust (WT101597MA, UK). Born in Bradford (BiB) is only possible because of the enthusiasm and commitment of the children and parents in BiB. We are grateful to all the participants, health professionals, schools and researchers who have made BiB happen. BiB receives funding from the ESRC/MRC, the Wellcome Trust (WT101597MA) and the National Institute for Health Research Yorkshire and Humber ARC (reference: NIHR20016). M. Mon-Williams was supported by a Fellowship from the Alan Turing Institute. Additional funding from the National Institute of Environmental Health Science supported Dr Chatzi (R01ES030691, R01ES029944, R01ES030364, R21ES029681, and P30ES007048). The views expressed are those of the authors, and not necessarily those of the NHS or the NIHR. None of the funders were involved in designing the study, collecting the data, analyzing or interpreting the data, deciding to submit the article for publication, or the writing of the report., HESAM Université (HESAM)-HESAM Université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Singapore Institute for Clinical Sciences [Singapour] (SICS), Bradford Institute for Health Research [Bradford, UK], Bradford Teaching Hospitals NHS Foundation Trust [Bradford, UK] (BTHFT), University of Leeds, Universitat de València (UV), Karolinska Institutet [Stockholm], Binter, Anne-Claire, Child and Adolescent Psychiatry / Psychology, Erasmus MC other, European Commission, Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Santé et de la Recherche Médicale (INSERM)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Université de Rennes 1 (UR1), and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Université d'Angers (UA)
Subjects: Urban environment, 010501 environmental sciences, 01 natural sciences, 0302 clinical medicine, Cognition, Pregnancy, Cognició en els infants, 11. Sustainability, GE1-350, 030212 general & internal medicine, Children, Motor skill, ComputingMilieux_MISCELLANEOUS, Desenvolupament urbà, General Environmental Science, SDG 15 - Life on Land, 2. Zero hunger, education.field_of_study, Air Pollutants, 4. Education, motor function, Cohort, cohort, SDG 11 - Sustainable Cities and Communities, 3. Good health, Child, Preschool, Birth Cohort, Female, Cognitive function, Psychology, Population, Gross motor skill, 03 medical and health sciences, children, Urban planning, Air Pollution, medicine, Humans, education, Motor function, cognitive function, 0105 earth and related environmental sciences, Environmental Exposure, medicine.disease, urban environment, Confidence interval, Environmental sciences, Spain, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, Particulate Matter, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Demography
Abstract: [EN]Background: The urban environment may influence neurodevelopment from conception onwards, but there is no evaluation of the impact of multiple groups of exposures simultaneously. We investigated the association between early-life urban environment and cognitive and motor function in children. Methods: We used data from 5403 mother-child pairs from four population-based birth-cohorts (UK, France, Spain, and Greece). We estimated thirteen urban home exposures during pregnancy and childhood, including: built environment, natural spaces, and air pollution. Verbal, non-verbal, gross motor, and fine motor functions were assessed using validated tests at five years old. We ran adjusted multi-exposure models using the Deletion-Substitution-Addition algorithm. Results: Higher greenness exposure within 300 m during pregnancy was associated with higher verbal abilities (1.5 points (95% confidence interval 0.4, 2.7) per 0.20 unit increase in greenness). Higher connectivity density within 100 m and land use diversity during pregnancy were related to lower verbal abilities. Childhood exposure to PM2.5 mediated 74% of the association between greenness during childhood and verbal abilities. Higher exposure to PM2.5 during pregnancy was related to lower fine motor function (-1.2 points (-2.1, -0.4) per 3.2 mu g/m3 increase in PM2.5). No associations were found with non-verbal abilities and gross motor function. Discussion: This study suggests that built environment, greenness, and air pollution may impact child cognitive and motor function at five years old. This study adds evidence that well-designed urban planning may benefit children's cognitive and motor development. Acknowledgements We are grateful to all the participating children, parents, practi-tioners and researchers in the four countries who took part in this study. This work was supported by funding from the European Community's Seventh Framework Programme [FP7/2007-206 n 308333; the HELIX project] . This INMA cohort was funded by grants from Instituto de Salud Carlos III (Red INMA G03/176; CB06/02/0041; PI041436; PI081151 incl. FEDER funds, FIS PI06/0867, FIS-PI09/00090, FIS and FIS-PI18/01142 incl. FEDER funds, FIS-FEDER: PI03/1615, PI04/1509, PI04/1112, PI04/1931, PI05/1079, PI05/1052, PI06/1213, PI07/0314, PI09/02647, PI11/01007, PI11/02591, PI11/02038, PI13/1944, PI13/2032, PI14/00891, PI14/01687, PI16/1288, PI16/00118 and PI17/00663; FIS-FSE: 17/00260; Miguel Servet-FEDER CP11/00178, CP15/00025, CPII16/00051, and CPII18/00018) , from UE (FP7-ENV-2011 cod 282957, HEALTH.2010.2.4.5-1, and H2020 n 824989) , Generalitat de Catalunya-CIRIT 1999SGR 00241, Fundacio La marato de TV3 (090430) , Generalitat Valenciana: FISABIO (UGP 15-230, UGP-15-244, and UGP-15-249) , Alicia Koplowitz Foundation 2017, CIBERESP, Department of Health of the Basque Government (2013111089, 2009111069, 2013111089, 2015111065 and 2018111086) , Provincial Government of Gipuzkoa (DFG06/002, DFG08/001, DFG15/221 and DFG 89/17) and annual agreements with the municipalities of the study area (Zumarraga, Urretxu , Legazpi, Azkoitia y Azpeitia y Beasain) . We acknowledge support from the Spanish Ministry of Science and Inno-vation and the State Research Agency through the "Centro de Excelencia Severo Ochoa 2019-2023" Program (CEX2018-000806-S) , and support from the Generalitat de Catalunya through the CERCA Program. The work was also supported by MICINN [MTM2015-68140-R] and Centro Nacional de Genotipado-CEGEN-PRB2-ISCIII (Spain) . The Rhea project was financially supported by European projects, and the Greek Ministry of Health (Program of Prevention of obesity and neurodevelopmental disorders in preschool children, in Heraklion district, Crete, Greece: 2011-2014; "Rhea Plus": Primary Prevention Program of Environmental Risk Factors for Reproductive Health, and Child Health: 2012-15) . This paper presents independent research funded by the National Institute for Health Research (NIHR) under its Collaboration for Applied Health Research and Care (CLAHRC) for Yorkshire and Humber (UK) . The EDEN study was supported by Foundation for medical research (FRM) , National Agency for Research (ANR) , National Institute for Research inPublic health (IRESP: TGIR cohorte sante 2008 program) , French Min-istry of Health (DGS) , French Ministry of Research, INSERM Bone and Joint Diseases National Research (PRO-A) , and Human Nutrition Na-tional Research Programs, Paris-Sud University, Nestle, French National Institute for Population Health Surveillance (InVS) , French National Institute for Health Education (INPES) , the European Union FP7 pro-grams (ESCAPE, ENRIECO, Medall projects) , Diabetes National Research Program (through a collaboration with the French Association of Diabetic Patients (AFD) ) , French Agency for Environmental Health Safety and French National Agency for Food Security (now ANSES) , Mutuelle Generale de l'Education Nationale a complementary health insurance (MGEN) , French-speaking association for the study of diabetes and metabolism (ALFEDIAM) . Core support for Born in Bradford is also provided by the Wellcome Trust (WT101597MA, UK) . Born in Bradford (BiB) is only possible because of the enthusiasm and commitment of the children and parents in BiB. We are grateful to all the participants, health professionals, schools and researchers who have made BiB happen. BiB receives funding from the ESRC/MRC, the Wellcome Trust (WT101597MA) and the National Institute for Health Research York-shire and Humber ARC (reference: NIHR20016) . M. Mon-Williams was supported by a Fellowship from the Alan Turing Institute. Additional funding from the National Institute of Environmental Health Science supported Dr Chatzi (R01ES030691, R01ES029944, R01ES030364, R21ES029681, and P30ES007048) . The views expressed are those of the authors, and not necessarily those of the NHS or the NIHR. None of the funders were involved in designing the study, collecting the data, analyzing or interpreting the data, deciding to submit the article for publication, or the writing of the report. Data sharing statement The HELIX data warehouse has been established as an accessible resource for collaborative research involving researchers external to the project. Access to HELIX data is based on approval by the HELIX Project Executive Committee and by the individual cohorts. Further details on the content of the data warehouse (data catalogue) and procedures for external access are described on the project website (http:// www.proj-ecthelix.eu/index.php/es/data-inventory) .
Published: 2022
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96. Sorting Nexin 27 Enables MTOC and Secretory Machinery Translocation to the Immune Synapse

Author: Natalia González-Mancha, Cristina Rodríguez-Rodríguez, Andrés Alcover, Isabel Merida, JEANNOT, FLORENCE, Innovative doctoral programme for talented early-stage researchers in Spanish host organisations excellent in the areas of Science, Technology, Engineering and Mathematics (STEM). H2020-EU.1.3.4 - INPhINIT - 713673 - INCOMING, Centro Nacional de Biotecnología [Madrid] (CNB-CSIC), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Biologie Cellulaire des Lymphocytes - Lymphocyte Cell Biology, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), NG-M received funding from the European Union Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement 713673 and 'La Caixa' Foundation (ID 100010434), with the fellowship code being LCF/BQ/DI17/11620027, as well as an EMBO short-term fellowship. CR-R held a predoctoral fellow of the Álvaro Entrecanales and Jérôme Lejeune Foundations. Research in IM’s lab is funded by grants from the Spanish Association Against Cancer (AECC, CICPF18), Aplastic Anemia and MDS International Foundation (AAMDSIF, OPE01644), Spanish Ministry of Science and Innovation (PID2019-108357RB-100/AEI/10.13039/501100011033), and the Madrid regional government (IMMUNOTHERCAM Consortium S2010/BMD-2326) to IM. Research in AA’s lab is funded by the French Ligue Nationale Contre le Cancer, Equipe Labellisée 2018)., and European Project: 713673,INPhINIT
Subjects: Diacylglycerol Kinase, Immunological Synapses, T-Lymphocytes, [SDV]Life Sciences [q-bio], Immunology, diacylglycerol kinase z, Vesicular Transport Proteins, T lymphocytes, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Lymphocyte Activation, Models, Biological, immune response, Diglycerides, Jurkat Cells, Cell Line, Tumor, Immunology and Allergy, Humans, Sorting Nexins, SNX27, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, Original Research, polarization, Secretory Pathway, Biological Transport, RC581-607, [SDV] Life Sciences [q-bio], centrosome, Protein Biosynthesis, Cytokines, diacylglycerol kinase ζ, Immunologic diseases. Allergy, retromer, Microtubule-Organizing Center, Signal Transduction
Abstract: Sorting nexin 27 (SNX27) association to the retromer complex mediates intracellular trafficking of cargoes containing PSD95/Dlg1/ZO-1 (PDZ)-binding C-terminal sequences from endosomes to the cell surface, preventing their lysosomal degradation. Antigen recognition by T lymphocyte leads to the formation of a highly organized structure named the immune synapse (IS), which ensures cell-cell communication and sustained T cell activation. At the neuronal synapse, SNX27 recycles PDZ-binding receptors and its defective expression is associated with synaptic dysfunction and cognitive impairment. In T lymphocytes, SNX27 was found localized at recycling endosomal compartments that polarized to the IS, suggesting a function in polarized traffic to this structure. Proteomic analysis of PDZ-SNX27 interactors during IS formation identify proteins with known functions in cytoskeletal reorganization and lipid regulation, such as diacylglycerol (DAG) kinase (DGK) ζ, as well as components of the retromer and WASH complex. In this study, we investigated the consequences of SNX27 deficiency in cytoskeletal reorganization during IS formation. Our analyses demonstrate that SNX27 controls the polarization towards the cell-cell interface of the PDZ-interacting cargoes DGKζ and the retromer subunit vacuolar protein sorting protein 26, among others. SNX27 silencing abolishes the formation of a DAG gradient at the IS and prevents re-localization of the dynactin complex component dynactin-1/p150Glued, two events that correlate with impaired microtubule organizing center translocation (MTOC). SNX27 silenced cells show marked alteration in cytoskeleton organization including a failure in the organization of the microtubule network and defects in actin clearance at the IS. Reduced SNX27 expression was also found to hinder the arrangement of signaling microclusters at the IS, as well as the polarization of the secretory machinery towards the antigen presenting cells. Our results broaden the knowledge of SNX27 function in T lymphocytes by showing a function in modulating IS organization through regulated trafficking of cargoes.
Published: 2022
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97. The risk of COVID-19 death is much greater and age-dependent with type I IFN autoantibodies

Author: Manry, Jérémy, Bastard, Paul, Gervais, Adrian, Le Voyer, Tom, Rosain, Jérémie, Philippot, Quentin, Michailidis, Eleftherios, Hoffmann, Hans-Heinrich, Eto, Shohei, Garcia-Prat, Marina, Bizien, Lucy, Parra-Martínez, Alba, Yang, Rui, Haljasmägi, Liis, Migaud, Mélanie, Särekannu, Karita, Maslovskaja, Julia, de Prost, Nicolas, Tandjaoui-Lambiotte, Yacine, Luyt, Charles-Edouard, Amador-Borrero, Blanca, Gaudet, Alexandre, Poissy, Julien, Morel, Pascal, Richard, Pascale, Cognasse, Fabrice, Troya, Jesús, Trouillet-Assant, Sophie, Belot, Alexandre, Saker, Kahina, Garçon, Pierre, Rivière, Jacques, Lagier, Jean-Christophe, Gentile, Stéphanie, Rosen, Lindsey, Shaw, Elana, Morio, Tomohiro, Tanaka, Junko, Dalmau, David, Tharaux, Pierre-Louis, Sene, Damien, Stepanian, Alain, Mégarbane, Bruno, Triantafyllia, Vasiliki, Fekkar, Arnaud, Heath, James, Franco, José Luis, Anaya, Juan-Manuel, Solé-Violán, Jordi, Imberti, Luisa, Biondi, Andrea, Bonfanti, Paolo, Castagnoli, Riccardo, Delmonte, Ottavia, Zhang, Yu, Snow, Andrew, Holland, Steven, Biggs, Catherine, Moncada-Vélez, Marcela, Arias, Andrés Augusto, Lorenzo, Lazaro, Boucherit, Soraya, Anglicheau, Dany, Planas, Anna, Haerynck, Filomeen, Duvlis, Sotirija, Ozcelik, Tayfun, Keles, Sevgi, Bousfiha, Ahmed, El Bakkouri, Jalila, Ramirez-Santana, Carolina, Paul, Stéphane, Pan-Hammarström, Qiang, Hammarström, Lennart, Dupont, Annabelle, Kurolap, Alina, Metz, Christine, Aiuti, Alessandro, Casari, Giorgio, Lampasona, Vito, Ciceri, Fabio, Barreiros, Lucila, Dominguez-Garrido, Elena, Vidigal, Mateus, Zatz, Mayana, van de Beek, Diederik, Sahanic, Sabina, Tancevski, Ivan, Stepanovskyy, Yurii, Boyarchuk, Oksana, Nukui, Yoko, Tsumura, Miyuki, Vidaur, Loreto, Tangye, Stuart, Burrel, Sonia, Duffy, Darragh, Quintana-Murci, Lluis, Klocperk, Adam, Kann, Nelli, Shcherbina, Anna, Lau, Yu-Lung, Leung, Daniel, Coulongeat, Matthieu, Marlet, Julien, Koning, Rutger, Reyes, Luis Felipe, Chauvineau-Grenier, Angélique, Venet, Fabienne, Monneret, Guillaume, Nussenzweig, Michel, Arrestier, Romain, Boudhabhay, Idris, Baris-Feldman, Hagit, Hagin, David, Wauters, Joost, Meyts, Isabelle, Dyer, Adam, Kennelly, Sean, Bourke, Nollaig, Halwani, Rabih, Sharif-Askari, Fatemeh Saheb, Dorgham, Karim, Sallette, Jérôme, Sedkaoui, Souad Mehlal, Alkhater, Suzan, Rigo-Bonnin, Raúl, Morandeira, Francisco, Roussel, Lucie, Vinh, Donald, Erikstrup, Christian, Condino-Neto, Antonio, Prando, Carolina, Bondarenko, Anastasiia, Spaan, András, Gilardin, Laurent, Fellay, Jacques, Lyonnet, Stanislas, Bilguvar, Kaya, Lifton, Richard, Mane, Shrikant, Anderson, Mark, Boisson, Bertrand, Béziat, Vivien, Zhang, Shen-Ying, Andreakos, Evangelos, Hermine, Olivier, Pujol, Aurora, Peterson, Pärt, Mogensen, Trine, Rowen, Lee, Mond, James, Debette, Stéphanie, de Lamballerie, Xavier, Burdet, Charles, Bouadma, Lila, Zins, Marie, Soler-Palacin, Pere, Colobran, Roger, Gorochov, Guy, Solanich, Xavier, Susen, Sophie, Martinez-Picado, Javier, Raoult, Didier, Vasse, Marc, Gregersen, Peter, Piemonti, Lorenzo, Rodríguez-Gallego, Carlos, Notarangelo, Luigi, Su, Helen, Kisand, Kai, Okada, Satoshi, Puel, Anne, Jouanguy, Emmanuelle, Rice, Charles, Tiberghien, Pierre, Zhang, Qian, Casanova, Jean-Laurent, Abel, Laurent, Cobat, Aurélie, Zhang, Peng, Seeleuthner, Yoann, Talouarn, Estelle, Marchal, Astrid, Matuozzo, Daniela, de la Chapelle, Aliénor, Chen, Jie, Chrabieh, Maya, Liu, Dana, Nemirowskaya, Yelena, Cruz, Inés Marín, Materna, Marie, Pelet, Sophie, Thibault, Chloé, Liu, Zhiyong, Abad, Jorge, Accordino, Giulia, Achille, Cristian, Aguilera-Albesa, Sergio, Aguiló-Cucurull, Aina, Özkan, Esra Akyüz, Darazam, Ilad Alavi, Albisures, Jonathan Antonio Roblero, Aldave, Juan, Ramos, Miquel Alfonso, Khan, Taj Ali, Aliberti, Anna, Nadji, Seyed Alireza, Alkan, Gulsum, Allardet-Servent, Jerome, Allende, Luis, Alonso-Arias, Rebeca, Alshahrani, Mohammed, Alsina, Laia, Alyanakian, Marie-Alexandra, Borrero, Blanca Amador, Amoura, Zahir, Antolí, Arnau, Aubart, Mélodie, Auguet, Teresa, Avramenko, Iryna, Aytekin, Gökhan, Azot, Axelle, Bahram, Seiamak, Bajolle, Fanny, Baldanti, Fausto, Baldolli, Aurélie, Ballester, Maite, Feldman, Hagit Baris, Barrou, Benoit, Barzaghi, Federica, Basso, Sabrina, Bayhan, Gulsum Iclal, Bezrodnik, Liliana, Bilbao, Agurtzane, Blanchard-Rohner, Geraldine, Blanco, Ignacio, Blandinières, Adeline, Blázquez-Gamero, Daniel, Bleibtreu, Alexandre, Bloomfield, Marketa, Bolivar-Prados, Mireia, Borghesi, Alessandro, Borie, Raphael, Botdhlo-Nevers, Elisabeth, Bousquet, Aurore, Boutolleau, David, Bouvattier, Claire, Bravais, Juliette, Briones, M. Luisa, Brunner, Marie-Eve, Bruno, Raffaele, Bueno, Maria Rita P., Bukhari, Huda, Bustamante, Jacinta, Agra, Juan José Cáceres, Capra, Ruggero, Carapito, Raphael, Carrabba, Maria, Casasnovas, Carlos, Caseris, Marion, Cassaniti, Irene, Castelle, Martin, Castelli, Francesco, de Vera, Martín Castillo, Castro, Mateus, Catherinot, Emilie, Celik, Jale Bengi, Ceschi, Alessandro, Chalumeau, Martin, Charbit, Bruno, Cheng, Matthew, Clavé, Pere, Clotet, Bonaventura, Codina, Anna, Cohen, Yves, Comarmond, Cloé, Combes, Alain, Comoli, Patrizia, Corsico, Angelo, Coşkuner, Taner, Cvetkovski, Aleksandar, Cyrus, Cyril, Danion, François, Darley, David Ross, Das, Vincent, Dauby, Nicolas, Dauger, Stéphane, de Munter, Paul, de Pontual, Loic, Dehban, Amin, Delplancq, Geoffroy, Demoule, Alexandre, Desguerre, Isabelle, Di Sabatino, Antonio, Diehl, Jean-Luc, Dobbelaere, Stephanie, Domínguez-Garrido, Elena, Dubost, Clément, Ekwall, Olov, Bozdemir, Şefika Elmas, Elnagdy, Marwa, Emiroglu, Melike, Endo, Akifumi, Erdeniz, Emine Hafize, Aytekin, Selma Erol, Lasa, Maria Pilar Etxart, Euvrard, Romain, Fabio, Giovanna, Faivre, Laurence, Falck, Antonin, Fartoukh, Muriel, Faure, Morgane, Arquero, Miguel Fernandez, Ferrer, Ricard, Ferreres, Jose, Flores, Carlos, Francois, Bruno, Fumadó, Victoria, Fung, Kitty, Fusco, Francesca, Gagro, Alenka, Solis, Blanca Garcia, Gaussem, Pascale, Gayretli, Zeynep, Gil-Herrera, Juana, Gatineau, Audrey Giraud, Girona-Alarcón, Mònica, Godínez, Karen Alejandra Cifuentes, Goffard, Jean-Christophe, Gonzales, Nacho, Gonzalez-Granado, Luis, González-Montelongo, Rafaela, Guerder, Antoine, Gülhan, Belgin, Gumucio, Victor Daniel, Hanitsch, Leif Gunnar, Gunst, Jan, Gut, Marta, Hadjadj, Jérôme, Hancerli, Selda, Hariyan, Tetyana, Hatipoglu, Nevin, Heppekcan, Deniz, Hernandez-Brito, Elisa, Ho, Po-Ki, Holanda-Peña, María Soledad, Horcajada, Juan, Hraiech, Sami, Humbert, Linda, Hung, Ivan, Iglesias, Alejandro, Íñigo-Campos, Antonio, Jamme, Matthieu, Arranz, María Jesús, Jimeno, Marie-Thérèse, Jordan, Iolanda, Kanık-Yüksek, Saliha, Kara, Yalcin Burak, Karahan, Aydın, Karbuz, Adem, Yasar, Kadriye Kart, Kasapcopur, Ozgur, Kashimada, Kenichi, Demirkol, Yasemin Kendir, Kido, Yasutoshi, Kizil, Can, Kılıç, Ahmet Osman, Daganou, Maria, Koukaki, Evangelia, Koutsoukou, Antonia, Rapti, Vasiliki, Syrigos, Konstantinos, Król, Zbigniew, Ksouri, Hatem, Kuentz, Paul, Kwan, Arthur, Kwan, Yat Wah M., Kwok, Janette, Lam, David, Conti, Francesca, Pession, Andrea, Lampropoulou, Vicky, Lanternier, Fanny, Bourgeois, Fleur Le, Leo, Yee-Sin, Lopez, Rafael Leon, Levin, Michael, Levy, Michael, Lévy, Romain, Li, Zhi, Lilleri, Daniele, Lima, Edson Jose Adrian Bolanos, Linglart, Agnes, López-Collazo, Eduardo, Lorenzo-Salazar, José, Louapre, Céline, Lubetzki, Catherine, Lung, Kwok-Cheung, Lye, David, Magnone, Cinthia, Mansouri, Davood, Marchioni, Enrico, Marioli, Carola, Marjani, Majid, Marques, Laura, Pereira, Jesus Marquez, Martín-Nalda, Andrea, Pueyo, David Martínez, Marzana, Iciar, Mata-Martínez, Carmen, Mathian, Alexis, Matos, Larissa Rb, Matthews, Gail, Mayaux, Julien, Mclaughlin-Garcia, Raquel, Meersseman, Philippe, Mège, Jean-Louis, Mekontso-Dessap, Armand, Melki, Isabelle, Meloni, Federica, Meritet, Jean-François, Merlani, Paolo, Akcan, Özge Metin, Mezidi, Mehdi, Migeotte, Isabelle, Millereux, Maude, Million, Matthieu, Mirault, Tristan, Mircher, Clotilde, Mirsaeidi, Mehdi, Mizoguchi, Yoko, Modi, Bhavi, Mojoli, Francesco, Moncomble, Elsa, Melián, Abián Montesdeoca, Martinez, Antonio Morales, Morange, Pierre-Emmanuel, Mordacq, Clémence, Morelle, Guillaume, Mouly, Stéphane, Muñoz-Barrera, Adrián, Nafati, Cyril, Nagashima, Shintaro, Nakagama, Yu, Neven, Bénédicte, Neves, João Farela, Ng, Lisa, Ng, Yuk-Yung, Nielly, Hubert, Medina, Yeray Novoa, Cuadros, Esmeralda Nuñez, Ocejo-Vinyals, J. Gonzalo, Okamoto, Keisuke, Oualha, Mehdi, Ouedrani, Amani, Özçelik, Tayfun, Ozkaya-Parlakay, Aslinur, Pagani, Michele, Papadaki, Maria, Parizot, Christophe, Parola, Philippe, Pascreau, Tiffany, Paz-Artal, Estela, Pedraza-Sánchez, Sigifredo, Gálvez-Romero, José Luis, Pellecer, Nancy Carolina González, Pellegrini, Silvia, Diego, Rebeca Pérez De, Pérez-Fernández, Xosé Luis, Philippe, Aurélien, Picod, Adrien, de Chambrun, Marc Pineton, Piralla, Antonio, Planas-Serra, Laura, Ploin, Dominique, Poncelet, Géraldine, Poulakou, Garyphallia, Pouletty, Marie, Pourshahnazari, Persia, Qiu-Chen, Jia Li, Quentric, Paul, Rambaud, Thomas, Raoult, Violette, Rebillat, Anne-Sophie, Redin, Claire, Resmini, Léa, Ricart, Pilar, Richard, Jean-Christophe, Rivet, Nadia, Rocamora-Blanch, Gemma, Rodero, Mathieu, Rodrigo, Carlos, Rodriguez, Luis Antonio, Rodriguez-Gallego, Carlos, Rodriguez-Palmero, Agustí, Romero, Carolina Soledad, Rothenbuhler, Anya, Roux, Damien, Rovina, Nikoletta, Rozenberg, Flore, Ruch, Yvon, Ruiz, Montse, Prado, Maria Yolanda Ruiz Del, Ruiz-Rodriguez, Juan Carlos, Sabater-Riera, Joan, Saks, Kai, Salagianni, Maria, Sanchez, Oliver, Sánchez-Montalvá, Adrián, Sánchez-Ramón, Silvia, Schidlowski, Laire, Schluter, Agatha, Schmidt, Julien, Schmidt, Matthieu, Schuetz, Catharina, Schweitzer, Cyril, Scolari, Francesco, Sediva, Anna, Seijo, Luis, Seminario, Analia Gisela, Seng, Piseth, Senoglu, Sevtap, Seppänen, Mikko, Llovich, Alex Serra, Shahrooei, Mohammad, Siguret, Virginie, Siouti, Eleni, Smadja, David, Smith, Nikaia, Sobh, Ali, Soler, Catherine, Soler-Palacín, Pere, Sözeri, Betül, Stella, Giulia Maria, Stepanovskiy, Yuriy, Stoclin, Annabelle, Taccone, Fabio, Taupin, Jean-Luc, Tavernier, Simon, Terrier, Benjamin, Thiery, Guillaume, Thorball, Christian, Thorn, Karolina, Thumerelle, Caroline, Tipu, Imran, Tolstrup, Martin, Tomasoni, Gabriele, Toubiana, Julie, Alvarez, Josep Trenado, Tsang, Owen, Tserel, Liina, Tso, Eugene, Tucci, Alessandra, Öz, Şadiye Kübra Tüter, Ursini, Matilde Valeria, Utsumi, Takanori, Uzunhan, Yurdagul, Vabres, Pierre, Valencia-Ramos, Juan, van den Rym, Ana Maria, Vandernoot, Isabelle, Velez-Santamaria, Valentina, Veliz, Silvia Patricia Zuniga, Viel, Sébastien, Villain, Cédric, Vilaire-Meunier, Marie, Villar-García, Judit, Vincent, Audrey, Vogt, Guillaume, Voiriot, Guillaume, Volokha, Alla, Vuotto, Fanny, Wauters, Els, Wu, Alan, Wu, Tak-Chiu, Yahşi, Aysun, Yesilbas, Osman, Yildiz, Mehmet, Young, Barnaby, Yükselmiş, Ufuk, Ghirardello, Stefano, Zuccaro, Valentina, Andrés, Ana De, van Praet, Jens, Lambrecht, Bart, van Braeckel, Eva, Bosteels, Cédric, Hoste, Levi, Hoste, Eric, Bauters, Fré, Clercq, Jozefien De, Heijmans, Cathérine, Slabbynck, Hans, Naesens, Leslie, Florkin, Benoit, Boulanger, Cécile, Vanderlinden, Dimitri, Foti, Giuseppe, Bellani, Giacomo, Citerio, Giuseppe, Contro, Ernesto, Pesci, Alberto, Valsecchi, Maria Grazia, Cazzaniga, Marina, Danielson, Jeffrey, Dobbs, Kerry, Kashyap, Anuj, Ding, Li, Dalgard, Clifton, Sottini, Alessandra, Quaresima, Virginia, Quiros-Roldan, Eugenia, Rossi, Camillo, Bettini, Laura Rachele, D’angio, Mariella, Beretta, Ilaria, Montagna, Daniela, Licari, Amelia, Marseglia, Gian Luigi, Batten, Isabella, Reddy, Conor, Mcelheron, Matt, Noonan, Claire, Connolly, Emma, Fallon, Aoife, Storgaard, Merete, Jørgensen, Sofie, Pedersen, Ole Birger, Sørensen, Erik, Mikkelsen, Susan, Dinh, Khoa Manh, Larsen, Margit Anita Hørup, Paulsen, Isabella Worlewenut, von Stemann, Jakob Hjorth, Hansen, Morten Bagge, Ostrowski, Sisse Rye, Townsend, Liam, Ni Cheallaigh, Cliona, Bergin, Colm, Martin-Loeches, Ignacio, Dunne, Jean, Conlon, Niall, O’farrelly, Cliona, Allavena, Clotilde, Andrejak, Claire, Angoulvant, François, Azoulay, Cecile, Bachelet, Delphine, Bartoli, Marie, Basmaci, Romain, Behillill, Sylvie, Beluze, Marine, Benech, Nicolas, Benkerrou, Dehbia, Bhavsar, Krishna, Bitker, Laurent, Bouscambert-Duchamp, Maude, Paz, Pauline Caraux, Cervantes-Gonzalez, Minerva, Chair, Anissa, Chirouze, Catherine, Coelho, Alexandra, Cordel, Hugues, Couffignal, Camille, Couffin-Cadiergues, Sandrine, D’ortenzio, Eric, de Montmollin, Etienne, Debard, Alexa, Debray, Marie-Pierre, Deplanque, Dominique, Descamps, Diane, Desvallée, Mathilde, Diallo, Alpha, Diouf, Alphonsine, Dorival, Céline, Dubos, François, Duval, Xavier, Eloy, Philippine, Enouf, Vincent, Epaulard, Olivier, Esperou, Hélène, Esposito-Farase, Marina, Etienne, Manuel, Garot, Denis, Gault, Nathalie, Gaymard, Alexandre, Ghosn, Jade, Gigante, Tristan, Gilg, Morgane, Goehringer, François, Guedj, Jérémie, Hoctin, Alexandre, Hoffmann, Isabelle, Houas, Ikram, Hulot, Jean-Sébastien, Jaafoura, Salma, Kafif, Ouifiya, Kaguelidou, Florentia, Kali, Sabrina, Kerroumi, Younes, Khalil, Antoine, Khan, Coralie, Kimmoun, Antoine, Laine, Fabrice, Laouénan, Cédric, Laribi, Samira, Le, Minh, Le Bris, Cyril, Le Gac, Sylvie, Le Hingrat, Quentin, Le Mestre, Soizic, Le Nagard, Hervé, Lemaignen, Adrien, Lemee, Véronique, Lescure, François-Xavier, Letrou, Sophie, Levy, Yves, Lina, Bruno, Lingas, Guillaume, Lucet, Jean Christophe, Machado, Moïse, Malvy, Denis, Mambert, Marina, Manuel, Aldric, Mentré, France, Meziane, Amina, Mouquet, Hugo, Mullaert, Jimmy, Neant, Nadège, Nguyen, Duc, Noret, Marion, Papadopoulos, Aurélie, Paul, Christelle, Peiffer-Smadja, Nathan, Peigne, Vincent, Petrov-Sanchez, Ventzislava, Peytavin, Gilles, Pham, Huong, Picone, Olivier, Piquard, Valentine, Puéchal, Oriane, Rosa-Calatrava, Manuel, Rossignol, Bénédicte, Rossignol, Patrick, Roy, Carine, Schneider, Marion, Su, Richa, Tardivon, Coralie, Tellier, Marie-Capucine, Téoulé, François, Terrier, Olivier, Timsit, Jean-François, Tual, Christelle, Tubiana, Sarah, van der Werf, Sylvie, Vanel, Noémie, Veislinger, Aurélie, Visseaux, Benoit, Wiedemann, Aurélie, Yazdanpanah, Yazdan, Annereau, Jean-Philippe, Briseño-Roa, Luis, Gribouval, Olivier, Jaïs, Jean-Philippe, Pelet, Anna, Alcover, Andres, Aschard, Hugues, Bousso, Philippe, Brodin, Petter, Bruhns, Pierre, Cerf-Bensussan, Nadine, Cumano, Ana, D’enfert, Christophe, Deriano, Ludovic, Dillies, Marie-Agnès, Di Santo, James, Dromer, Françoise, Eberl, Gérard, Enninga, Jost, Gomperts-Boneca, Ivo, Hasan, Milena, Hedestam, Gunilla Karlsson, Hercberg, Serge, Ingersoll, Molly, Lantz, Olivier, Kenny, Rose Anne, Ménager, Mickaël, Michel, Frédérique, Patin, Etienne, Pellegrini, Sandra, Rausell, Antonio, Rieux-Laucat, Frédéric, Rogge, Lars, Fontes, Magnus, Sakuntabhai, Anavaj, Schwartz, Olivier, Schwikowski, Benno, Shorte, Spencer, Tangy, Frédéric, Toubert, Antoine, Touvier, Mathilde, Ungeheuer, Marie-Noëlle, Zimmer, Christophe, Albert, Matthew, Alavoine, Loubna, Behillil, Sylvie, Charpentier, Charlotte, Dechanet, Aline, Ecobichon, Jean-Luc, Frezouls, Wahiba, Houhou, Nadhira, Lehacaut, Jonathan, Lucet, Jean-Christophe, Manchon, Pauline, Nouroudine, Mariama, Quintin, Caroline, Thy, Michael, Vignali, Valérie, Chahine, Abir, Waucquier, Nawal, Migaud, Maria-Claire, Djossou, Félix, Mergeay-Fabre, Mayka, Lucarelli, Aude, Demar, Magalie, Bruneau, Léa, Gérardin, Patrick, Maillot, Adrien, Payet, Christine, Laviolle, Bruno, Paris, Christophe, Desille-Dugast, Mireille, Fouchard, Julie, Pistone, Thierry, Perreau, Pauline, Gissot, Valérie, Le Goas, Carole, Montagne, Samatha, Richard, Lucie, Bouiller, Kévin, Desmarets, Maxime, Meunier, Alexandre, Bourgeon, Marilou, Lefèvre, Benjamin, Jeulin, Hélène, Legrand, Karine, Lomazzi, Sandra, Tardy, Bernard, Gagneux-Brunon, Amandine, Bertholon, Frédérique, Botelho-Nevers, Elisabeth, Kouakam, Christelle, Leturque, Nicolas, Roufai, Layidé, Amat, Karine, Espérou, Hélène, Hendou, Samia, van Agtmael, Michiel, Algera, Anne Geke, Appelman, Brent, van Baarle, Frank, Bax, Diane, Beudel, Martijn, Bogaard, Harm Jan, Bomers, Marije, Bonta, Peter, Bos, Lieuwe, Botta, Michela, de Brabander, Justin, de Bree, Godelieve, de Bruin, Sanne, Buis, David, Bugiani, Marianna, Bulle, Esther, Chouchane, Osoul, Cloherty, Alex, Dijkstra, Mirjam, Dongelmans, Dave, Dujardin, Romein, Elbers, Paul, Fleuren, Lucas, Geerlings, Suzanne, Geijtenbeek, Theo, Girbes, Armand, Goorhuis, Bram, Grobusch, Martin, Hafkamp, Florianne, Hagens, Laura, Hamann, Jorg, Harris, Vanessa, Hemke, Robert, Hermans, Sabine, Heunks, Leo, Hollmann, Markus, Horn, Janneke, Hovius, Joppe, de Jong, Menno, Lim, Endry, van Mourik, Niels, Nellen, Jeaninne, Nossent, Esther, Paulus, Frederique, Peters, Edgar, Pina-Fuentes, Dan, van der Poll, Tom, Preckel, Bennedikt, Prins, Jan, Raasveld, Jorinde, Reijnders, Tom, de Rotte, Maurits, Schinkel, Michiel, Schultz, Marcus, Schrauwen, Femke, Schuurman, Alex, Schuurmans, Jaap, Sigaloff, Kim, Slim, Marleen, Smeele, Patrick, Smit, Marry, Stijnis, Cornelis, Stilma, Willemke, Teunissen, Charlotte, Thoral, Patrick, Tsonas, Anissa, Tuinman, Pieter, van der Valk, Marc, Veelo, Denise, Volleman, Carolien, de Vries, Heder, Vught, Lonneke, van Vugt, Michèle, Wouters, Dorien, Zwinderman, A., Brouwer, Matthijs, Wiersinga, W. Joost, Vlaar, Alexander, Al-Muhsen, Saleh, Al-Mulla, Fahd, Arias, Andrés, Bogunovic, Dusan, Bolze, Alexandre, Bryceson, Yenan, Bustamante, Carlos, Butte, Manish, Chakravorty, Samya, Christodoulou, John, Constantinescu, Stefan, Cooper, Megan, Desai, Murkesh, Drolet, Beth, El Baghdadi, Jamila, Espinosa-Padilla, Sara, Froidure, Antoine, Henrickson, Sarah, Hsieh, Elena, Husebye, Eystein, Imai, Kohsuke, Itan, Yuval, Jarvis, Erich, Karamitros, Timokratis, Ku, Cheng-Lung, Ling, Yun, Lucas, Carrie, Maniatis, Tom, Maródi, László, Milner, Joshua, Mironska, Kristina, Novelli, Antonio, Novelli, Giuseppe, de Diego, Rebeca Perez, Perez-Tur, Jordi, Arkin, Lisa, Asano, Takaki, Oriol, Roger Colobran, Renia, Laurent, Resnick, Igor, Sancho-Shimizu, Vanessa, Seppänen, Mikko R.J., Shahrooei, Mohammed, Slaby, Ondrej, Tayoun, Ahmad Abou, Ramaswamy, Sathishkumar, Turvey, Stuart, Uddin, K., Uddin, Mohammed, von Bernuth, Horst, Zawadzki, Pawel, Grimbacher, Bodo, Pape, Jean, Perlin, David, Pesole, Graziano, García, Paula Andrea Gaviria, López, Gustavo Andrés Salguero, Rojas-Villaraga, Adriana, Vélez, Verónica Posada, Landinez, Lina Marcela Acevedo, Correales, Luisa Paola Duarte, Gómez, Oscar, Guaqueta, Jeser Santiago Grass, Pérez, Cristian Alejandro Ricaurte, Carrillo, Jorge, Vergara, José Alejandro Daza, Landinez, Sandra, Mantilla, Rubén, Yepes, Jairo David Torres, Ricaurte, Oscar Andrés Briceño, Pérez-Díaz, Carlos, Mateus, Yady Nataly, Navarro, Laura Mancera, Rodríguez, Yhojan, Acosta-Ampudia, Yeny, Monsalve, Diana, Rojas, Manuel, Nadif, Rachel, Goldberg, Marcel, Ozguler, Anna, Henny, Joseph, Lemonnier, Sylvie, Coeuret-Pellicer, Mireille, Got, Stéphane Le, Tzourio, Christophe, Dufouil, Carole, Soumaré, Aïcha, Lachaize, Morgane, Fievet, Nathalie, Flaig, Amandine, Martin, Fernando, Bonneaudeau, Brigitte, Cannet, Dorothée, Gallian, Pierre, Jeanne, Michel, Perroquin, Magali, Hamzeh-Cognasse, Hind, Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Rockefeller University [New York], Hiroshima University, Vall d’Hebron Research Institute (VHIR), University of Tartu, CHU Henri Mondor, Hôpital Avicenne [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut de cardiologie [CHU Pitié-Salpêtrière], CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Hôpital Lariboisière-Fernand-Widal [APHP], Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Etablissement Français du Sang [La Plaine Saint-Denis] (EFS), Etablissement français du sang - Auvergne-Rhône-Alpes (EFS), Hospital Universitario Infanta Leonor [Madrid], Hospices Civils de Lyon (HCL), Grand Hôpital de l'Est Francilien (GHEF), Vall d'Hebron University Hospital [Barcelona], Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Assistance Publique - Hôpitaux de Marseille (APHM), National Institutes of Health [Bethesda] (NIH), Tokyo Medical and Dental University [Japan] (TMDU), Universitat de Barcelona (UB), Institut National de la Santé et de la Recherche Médicale (INSERM), Biomedical Research Foundation of the Academy of Athens (BRFAA), Institute for Systems Biology [Seattle] (ISB), Universidad de Antioquia = University of Antioquia [Medellín, Colombia], Universidad del Rosario [Bogota], Hospital Universitario de Gran Canaria Dr Negrin, Azienda Socio Sanitaria Territoriale Spedali Civili di Brescia [Brescia], Università degli Studi di Milano-Bicocca = University of Milano-Bicocca (UNIMIB), National Institute of Allergy and Infectious Diseases [Bethesda] (NIAID-NIH), Uniformed Services University of the Health Sciences (USUHS), University of British Columbia (UBC), CHU Necker - Enfants Malades [AP-HP], Spanish National Research Council (CSIC), Ghent University Hospital, Goce Delchev University (UGD), Invitae Corporation, Bilkent University [Ankara], Necmettin Erbakan University [Konya, Turquie], Centre Hospitalier Universitaire Hassan II (CHU HII), CHU Ibn Rochd [Casablanca], Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Karolinska Institute, Réseau International des Instituts Pasteur (RIIP), Tel Aviv Sourasky Medical Center [Te Aviv], The Feinstein Institute for Medical Research, IRCCS San Raffaele Scientific Institute [Milan, Italie], IRCCS Ospedale San Raffaele [Milan, Italy], Universidade de São Paulo = University of São Paulo (USP), Fundacion Rioja Salud, Amsterdam Neuroscience [Pays-Bas], Vrije Universiteit Amsterdam [Amsterdam] (VU)-University of Amsterdam [Amsterdam] (UvA)-VU University Medical Center [Amsterdam], Innsbruck Medical University = Medizinische Universität Innsbruck (IMU), Shupyk National Medical Academy of Postgraduate Education [Kiev] (SNMAPE), I.Horbachevsky Ternopil State Medical University, Ternopil, Ukraine, Hospital Donostia, Garvan Institute of medical research, Sorbonne Université (SU), Immunologie Translationnelle - Translational Immunology lab, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Institut Pasteur [Paris] (IP), Charles University [Prague] (CU), University Hospital Motol [Prague], Dmitriy Rogachev National Center for Pediatric Hematology, Oncology and Immunology [Moscow, Russia], The University of Hong Kong (HKU), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Morphogénèse et antigénicité du VIH et du virus des Hépatites (MAVIVH - U1259 Inserm - CHRU Tours ), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)-Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), University of La Sabana = Universitad de la Sabana, Hôpital Robert Ballanger [Aulnay-sous-Bois], Sackler Faculty of Medicine, Tel Aviv University (TAU), Catholic University of Leuven - Katholieke Universiteit Leuven (KU Leuven), University Hospitals Leuven [Leuven], Tallaght Hospital, Trinity College Dublin, University of Sharjah (UoS), Centre d'Immunologie et des Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Laboratoire CERBA [Saint Ouen l'Aumône], King Fahad University, Institut d'Investigació Biomèdica de Bellvitge [Barcelone] (IDIBELL), McGill University = Université McGill [Montréal, Canada], Aarhus University [Aarhus], Instituto de Pesquisa Pelé Pequeno Principe, Hôpital Jean Verdier [AP-HP], Ecole Polytechnique Fédérale de Lausanne (EPFL), Yale University [New Haven], University of California [San Francisco] (UC San Francisco), University of California (UC), Academy of Athens, Institució Catalana de Recerca i Estudis Avançats (ICREA), Bordeaux population health (BPH), Université de Bordeaux (UB)-Institut de Santé Publique, d'Épidémiologie et de Développement (ISPED)-Institut National de la Santé et de la Recherche Médicale (INSERM), Aix Marseille Université (AMU), Centre d'investigation Clinique [CHU Bichat] - Épidémiologie clinique (CIC 1425), AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM), CIC Hôpital Bichat, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-UFR de Médecine, Université Paris-Saclay, Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), European Genomic Institute for Diabetes - FR 3508 (EGID), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), IrsiCaixa (Institut de Recerca de la Sida), Hôpital Foch [Suresnes], Etablissement Français du Sang, EFS, Institut Necker Enfants-Malades (INEM - UM 111 (UMR 8253 / U1151)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Howard Hughes Medical Institute, Rockefeller University, St. Giles Foundation, National Institutes of Health (US), George Mason University, Yale University, National Human Genome Research Institute (US), Fisher Center for Alzheimer's Research Foundation, Meyer Foundation, JPB Foundation, Agence Nationale de la Recherche (France), Fondation pour la Recherche Médicale, Agence Nationale de Recherches sur le SIDA et les Hépatites Virales (France), Scientific Committee on Oceanic Research, Ministre de l'Enseignement Supérieur, de la Recherche et de l'Innovation (France), Institut National de la Santé et de la Recherche Médicale (France), Université de Paris, Fondation Bettencourt Schueller, Centro de Investigación Biomédica en Red Enfermedades Raras (España), European Commission, G. Harold & Leila Y. Mathers Foundation, Hospital Universitario Infanta Leonor, National Institute of Allergy and Infectious Diseases (US), National Institute of Dental and Craniofacial Research (US), Estonian Research Council, Al Jalila Foundation, American University of Sharjah, National Health and Medical Research Council (Australia), University of New South Wales (Australia), Regione Lombardia, Instituto de Salud Carlos III, Japan Agency for Medical Research and Development, Sorbonne Université, Université de Bordeaux, National Cancer Institute (US), Research Foundation - Flanders, Hellenic Foundation for Research and Innovation, Sao Paulo Research Foundation, The Meath Foundation, Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Research Unit on Cardiovascular and Metabolic Diseases (ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut de Cardiométabolisme et Nutrition = Institute of Cardiometabolism and Nutrition [CHU Pitié Salpêtrière] (IHU ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), INSERM UMRS-1144, Université Paris Cité, Réanimation Médicale et Toxicologique, Hôpital Lariboisière, Génétique Evolutive Humaine - Human Evolutionary Genetics, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), The Laboratory of Human Genetics of Infectious Diseases is supported by the Howard Hughes Medical Institute, The Rockefeller University, the St. Giles Foundation, the NIH (Grants R01AI088364 and R01AI163029), the National Center for Advancing Translational Sciences, NIH Clinical and Translational Science Awards program (Grant UL1 TR001866), a Fast Grant from Emergent Ventures, Mercatus Center at George Mason University, the Yale Center for Mendelian Genomics and the Genome Sequencing Program Coordinating Center funded by the National Human Genome Research Institute (Grants UM1HG006504 and U24HG008956), the Yale High Performance Computing Center (Grant S10OD018521), the Fisher Center for Alzheimer’s Research Foundation, the Meyer Foundation, the JPB Foundation, the French National Research Agency (ANR) under the 'Investments for the Future' program (Grant ANR-10-IAHU-01), the Integrative Biology of Emerging Infectious Diseases Laboratory of Excellence (Grant ANR-10-LABX-62-IBEID), the French Foundation for Medical Research (FRM) (Grant EQU201903007798), the French Agency for Research on AIDS and Viral hepatitis (ANRS) Nord-Sud (Grant ANRS-COV05), the ANR GENVIR (Grant ANR-20-CE93-003), AABIFNCOV (Grant ANR-20-CO11-0001), CNSVIRGEN (Grant ANR-19-CE15-0009-01), and GenMIS-C (Grant ANR-21-COVR-0039) projects, the Square Foundation, Grandir–Fonds de solidarité pour l’Enfance, the Fondation du Souffle, the SCOR Corporate Foundation for Science, The French Ministry of Higher Education, Research, and Innovation (Grant MESRI-COVID-19), Institut National de la Santé et de la Recherche Médicale (INSERM), REACTing-INSERM, and the University Paris Cité. P. Bastard was supported by the FRM (Award EA20170638020). P. Bastard., J.R., and T.L.V. were supported by the MD-PhD program of the Imagine Institute (with the support of Fondation Bettencourt Schueller). Work at the Neurometabolic Disease lab received funding from Centre for Biomedical Research on Rare Diseases (CIBERER) (Grant ACCI20-767) and the European Union's Horizon 2020 research and innovation program under grant agreement 824110 (EASI Genomics). Work in the Laboratory of Virology and Infectious Disease was supported by the NIH (Grants P01AI138398-S1, 2U19AI111825, and R01AI091707-10S1), a George Mason University Fast Grant, and the G. Harold and Leila Y. Mathers Charitable Foundation. The Infanta Leonor University Hospital supported the research of the Department of Internal Medicine and Allergology. The French COVID Cohort study group was sponsored by INSERM and supported by the REACTing consortium and by a grant from the French Ministry of Health (Grant PHRC 20-0424). The Cov-Contact Cohort was supported by the REACTing consortium, the French Ministry of Health, and the European Commission (Grant RECOVER WP 6). This work was also partly supported by the Intramural Research Program of the National Institute of Allergy and Infectious Diseases and the National Institute of Dental and Craniofacial Research, NIH (Grants ZIA AI001270 to L.D.N. and 1ZIAAI001265 to H.C.S.). This program is supported by the Agence Nationale de la Recherche (Grant ANR-10-LABX-69-01). K.K.’s group was supported by the Estonian Research Council, through Grants PRG117 and PRG377. R.H. was supported by an Al Jalila Foundation Seed Grant (Grant AJF202019), Dubai, United Arab Emirates, and a COVID-19 research grant (Grant CoV19-0307) from the University of Sharjah, United Arab Emirates. S.G.T. is supported by Investigator and Program Grants awarded by the National Health and Medical Research Council of Australia and a University of New South Wales COVID Rapid Response Initiative Grant. L.I. reports funding from Regione Lombardia, Italy (project 'Risposta immune in pazienti con COVID-19 e co-morbidità'). This research was partially supported by the Instituto de Salud Carlos III (Grant COV20/0968). J.R.H. reports funding from Biomedical Advanced Research and Development Authority (Grant HHSO10201600031C). S.O. reports funding from Research Program on Emerging and Re-emerging Infectious Diseases from Japan Agency for Medical Research and Development (Grant JP20fk0108531). G.G. was supported by the ANR Flash COVID-19 program and SARS-CoV-2 Program of the Faculty of Medicine from Sorbonne University iCOVID programs. The 3C Study was conducted under a partnership agreement between INSERM, Victor Segalen Bordeaux 2 University, and Sanofi-Aventis. The Fondation pour la Recherche Médicale funded the preparation and initiation of the study. The 3C Study was also supported by the Caisse Nationale d’Assurance Maladie des Travailleurs Salariés, Direction générale de la Santé, Mutuelle Générale de l’Education Nationale, Institut de la Longévité, Conseils Régionaux of Aquitaine and Bourgogne, Fondation de France, and Ministry of Research–INSERM Program 'Cohortes et collections de données biologiques.' S. Debette was supported by the University of Bordeaux Initiative of Excellence. P.K.G. reports funding from the National Cancer Institute, NIH, under Contract 75N91019D00024, Task Order 75N91021F00001. J.W. is supported by a Research Foundation - Flanders (FWO) Fundamental Clinical Mandate (Grant 1833317N). Sample processing at IrsiCaixa was possible thanks to the crowdfunding initiative YoMeCorono. Work at Vall d’Hebron was also partly supported by research funding from Instituto de Salud Carlos III Grant PI17/00660 cofinanced by the European Regional Development Fund (ERDF/FEDER). C.R.-G. and colleagues from the Canarian Health System Sequencing Hub were supported by the Instituto de Salud Carlos III (Grants COV20_01333 and COV20_01334), the Spanish Ministry for Science and Innovation (RTC-2017-6471-1, AEI/FEDER, European Union), Fundación DISA (Grants OA18/017 and OA20/024), and Cabildo Insular de Tenerife (Grants CGIEU0000219140 and 'Apuestas científicas del ITER para colaborar en la lucha contra la COVID-19'). T.H.M. was supported by grants from the Novo Nordisk Foundation (Grants NNF20OC0064890 and NNF21OC0067157). C.M.B. is supported by a Michael Smith Foundation for Health Research Health Professional-Investigator Award. P.Q.H. and L. Hammarström were funded by the European Union’s Horizon 2020 research and innovation program (Antibody Therapy Against Coronavirus consortium, Grant 101003650). Work at Y.-L.L.’s laboratory in the University of Hong Kong (HKU) was supported by the Society for the Relief of Disabled Children. MBBS/PhD study of D.L. in HKU was supported by the Croucher Foundation. J.L.F. was supported in part by the Evaluation-Orientation de la Coopération Scientifique (ECOS) Nord - Coopération Scientifique France-Colombie (ECOS-Nord/Columbian Administrative department of Science, Technology and Innovation [COLCIENCIAS]/Colombian Ministry of National Education [MEN]/Colombian Institute of Educational Credit and Technical Studies Abroad [ICETEX, Grant 806-2018] and Colciencias Contract 713-2016 [Code 111574455633]). A. Klocperk was, in part, supported by Grants NU20-05-00282 and NV18-05-00162 issued by the Czech Health Research Council and Ministry of Health, Czech Republic. L.P. was funded by Program Project COVID-19 OSR-UniSR and Ministero della Salute (Grant COVID-2020-12371617). I.M. is a Senior Clinical Investigator at the Research Foundation–Flanders and is supported by the CSL Behring Chair of Primary Immunodeficiencies (PID), by the Katholieke Universiteit Leuven C1 Grant C16/18/007, by a Flanders Institute for Biotechnology-Grand Challenges - PID grant, by the FWO Grants G0C8517N, G0B5120N, and G0E8420N, and by the Jeffrey Modell Foundation. I.M. has received funding under the European Union’s Horizon 2020 research and innovation program (Grant Agreement 948959). E.A. received funding from the Hellenic Foundation for Research and Innovation (Grant INTERFLU 1574). M. Vidigal received funding from the São Paulo Research Foundation (Grant 2020/09702-1) and JBS SA (Grant 69004). The NH-COVAIR study group consortium was supported by a grant from the Meath Foundation., HGID Lab, COVID Clinicians, COVID-STORM Clinicians, NIAID Immune Response to COVID Group, NH-COVAIR Study Group, Danish CHGE, Danish Blood Donor Study, St. James's Hospital, SARS CoV2 Interest Group, French COVID Cohort Study Group, Imagine COVID-Group, Milieu Intérieur Consortium, CoV-Contact Cohort, Amsterdam UMC Covid-19 Biobank Investigators, COVID Human Genetic Effort, CP-COVID-19 Group, CONSTANCES cohort, 3C-Dijon Study, Cerba Health-Care, Etablissement Français du Sang Study group, ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), ANR-20-COVI-0003,GENCOVID,Identification des défauts monogéniques de l'immunité responsables des formes sévères de COVID-19 chez les patients précédemment en bonne santé(2020), ANR-20-CE93-0003,GENVIR,Analyse multi-omique de l'immunité anti-virale: de l'identification des circuits biologiques pertinents à la découverte de défauts monogéniques héréditaires de l'immunité chez les patients avec infections virales sévères(2020), ANR-19-CE15-0009,CNSVIRGEN,Déficits immunitaires innés dans les infections sévères du tronc cérébral(2019), ANR-20-CO11-0001,AABIFNCOV,Bases génétiques et immunologiques des auto-anticorps contre les interférons de type I prédisposant aux formes sévères de COVID-19.(2020), ANR-21-COVR-0039,GenMIS-C,Recherche des Déficits immunitaires innées monogéniques prédisposant au syndrome inflammatoire multisystémique chez l'enfant.(2021), European Project: 948959,ERC-2020-STG,MORE2ADA2(2021), Manry, J, Bastard, P, Gervais, A, Le Voyer, T, Rosain, J, Philippot, Q, Michailidis, E, Hoffmann, H, Eto, S, Garcia-Prat, M, Bizien, L, Parra-Martínez, A, Yang, R, Haljasmägi, L, Migaud, M, Särekannu, K, Maslovskaja, J, de Prost, N, Tandjaoui-Lambiotte, Y, Luyt, C, Amador-Borrero, B, Gaudet, A, Poissy, J, Morel, P, Richard, P, Cognasse, F, Troya, J, Trouillet-Assant, S, Belot, A, Saker, K, Garçon, P, Rivière, J, Lagier, J, Gentile, S, Rosen, L, Shaw, E, Morio, T, Tanaka, J, Dalmau, D, Tharaux, P, Sene, D, Stepanian, A, Mégarbane, B, Triantafyllia, V, Fekkar, A, Heath, J, Franco, J, Anaya, J, Solé-Violán, J, Imberti, L, Biondi, A, Bonfanti, P, Castagnoli, R, Delmonte, O, Zhang, Y, Snow, A, Holland, S, Biggs, C, Moncada-Vélez, M, Arias, A, Lorenzo, L, Boucherit, S, Anglicheau, D, Planas, A, Haerynck, F, Duvlis, S, Ozcelik, T, Keles, S, Bousfiha, A, El Bakkouri, J, Ramirez-Santana, C, Paul, S, Pan-Hammarström, Q, Hammarström, L, Dupont, A, Kurolap, A, Metz, C, Aiuti, A, Casari, G, Lampasona, V, Ciceri, F, Barreiros, L, Dominguez-Garrido, E, Vidigal, M, Zatz, M, van de Beek, D, Sahanic, S, Tancevski, I, Stepanovskyy, Y, Boyarchuk, O, Nukui, Y, Tsumura, M, Vidaur, L, Tangye, S, Burrel, S, Duffy, D, Quintana-Murci, L, Klocperk, A, Kann, N, Shcherbina, A, Lau, Y, Leung, D, Coulongeat, M, Marlet, J, Koning, R, Reyes, L, Chauvineau-Grenier, A, Venet, F, Monneret, G, Nussenzweig, M, Arrestier, R, Boudhabhay, I, Baris-Feldman, H, Hagin, D, Wauters, J, Meyts, I, Dyer, A, Kennelly, S, Bourke, N, Halwani, R, Sharif-Askari, F, Dorgham, K, Sallette, J, Sedkaoui, S, Alkhater, S, Rigo-Bonnin, R, Morandeira, F, Roussel, L, Vinh, D, Erikstrup, C, Condino-Neto, A, Prando, C, Bondarenko, A, Spaan, A, Gilardin, L, Fellay, J, Lyonnet, S, Bilguvar, K, Lifton, R, Mane, S, Anderson, M, Boisson, B, Béziat, V, Zhang, S, Andreakos, E, Hermine, O, Pujol, A, Peterson, P, Mogensen, T, Rowen, L, Mond, J, Debette, S, de Lamballerie, X, Burdet, C, Bouadma, L, Zins, M, Soler-Palacin, P, Colobran, R, Gorochov, G, Solanich, X, Susen, S, Martinez-Picado, J, Raoult, D, Vasse, M, Gregersen, P, Piemonti, L, Rodríguez-Gallego, C, Notarangelo, L, Su, H, Kisand, K, Okada, S, Puel, A, Jouanguy, E, Rice, C, Tiberghien, P, Zhang, Q, Casanova, J, Abel, L, Cobat, A, Laboratory of Human Genetics of Infectious Diseases, Necker Branch, INSERM U1163, Imagine Institute, Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Unité des Virus Emergents (UVE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Cohortes épidémiologiques en population (CONSTANCES), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay-Université Paris Cité (UPCité), UFR Médecine [Santé] - Université Paris Cité (UFR Médecine UPCité), Université Paris Cité (UPCité), Neurology, AII - Infectious diseases, ANS - Neuroinfection & -inflammation, Graduate School, Université Paris Cité - UFR Médecine [Santé] (UPCité UFR Médecine), Gunst, Jan, Acibadem University Dspace, Manry, Jérémy, Bastard, Paul, Gervais, Adrian, Le Voyer, Tom, Rosain, Jérémie, Philippot, Quentin, Michailidis, Eleftherio, Hoffmann, Hans-Heinrich, Eto, Shohei, Garcia-Prat, Marina, Bizien, Lucy, Parra-Martínez, Alba, Yang, Rui, Haljasmägi, Lii, Migaud, Mélanie, Särekannu, Karita, Maslovskaja, Julia, de Prost, Nicola, Tandjaoui-Lambiotte, Yacine, Luyt, Charles-Edouard, Amador-Borrero, Blanca, Gaudet, Alexandre, Poissy, Julien, Morel, Pascal, Richard, Pascale, Cognasse, Fabrice, Troya, Jesú, Trouillet-Assant, Sophie, Belot, Alexandre, Saker, Kahina, Garçon, Pierre, Rivière, Jacques G, Lagier, Jean-Christophe, Gentile, Stéphanie, Rosen, Lindsey B, Shaw, Elana, Morio, Tomohiro, Tanaka, Junko, Dalmau, David, Tharaux, Pierre-Loui, Sene, Damien, Stepanian, Alain, Mégarbane, Bruno, Triantafyllia, Vasiliki, Fekkar, Arnaud, Heath, James R, Franco, José Lui, Anaya, Juan-Manuel, Solé-Violán, Jordi, Imberti, Luisa, Biondi, Andrea, Bonfanti, Paolo, Castagnoli, Riccardo, Delmonte, Ottavia M, Zhang, Yu, Snow, Andrew L, Holland, Steven M, Biggs, Catherine M, Moncada-Vélez, Marcela, Arias, Andrés Augusto, Lorenzo, Lazaro, Boucherit, Soraya, Anglicheau, Dany, Planas, Anna M, Haerynck, Filomeen, Duvlis, Sotirija, Ozcelik, Tayfun, Keles, Sevgi, Bousfiha, Ahmed A, El Bakkouri, Jalila, Ramirez-Santana, Carolina, Paul, Stéphane, Pan-Hammarström, Qiang, Hammarström, Lennart, Dupont, Annabelle, Kurolap, Alina, Metz, Christine N, Aiuti, Alessandro, Casari, Giorgio, Lampasona, Vito, Ciceri, Fabio, Barreiros, Lucila A, Dominguez-Garrido, Elena, Vidigal, Mateu, Zatz, Mayana, van de Beek, Diederik, Sahanic, Sabina, Tancevski, Ivan, Stepanovskyy, Yurii, Boyarchuk, Oksana, Nukui, Yoko, Tsumura, Miyuki, Vidaur, Loreto, Tangye, Stuart G, Burrel, Sonia, Duffy, Darragh, Quintana-Murci, Llui, Klocperk, Adam, Kann, Nelli Y, Shcherbina, Anna, Lau, Yu-Lung, Leung, Daniel, Coulongeat, Matthieu, Marlet, Julien, Koning, Rutger, Reyes, Luis Felipe, Chauvineau-Grenier, Angélique, Venet, Fabienne, Monneret, Guillaume, Nussenzweig, Michel C, Arrestier, Romain, Boudhabhay, Idri, Baris-Feldman, Hagit, Hagin, David, Wauters, Joost, Meyts, Isabelle, Dyer, Adam H, Kennelly, Sean P, Bourke, Nollaig M, Halwani, Rabih, Sharif-Askari, Fatemeh Saheb, Dorgham, Karim, Sallette, Jérôme, Sedkaoui, Souad Mehlal, Alkhater, Suzan, Rigo-Bonnin, Raúl, Morandeira, Francisco, Roussel, Lucie, Vinh, Donald C, Erikstrup, Christian, Condino-Neto, Antonio, Prando, Carolina, Bondarenko, Anastasiia, Spaan, András N, Gilardin, Laurent, Fellay, Jacque, Lyonnet, Stanisla, Bilguvar, Kaya, Lifton, Richard P, Mane, Shrikant, Anderson, Mark S, Boisson, Bertrand, Béziat, Vivien, Zhang, Shen-Ying, Andreakos, Evangelo, Hermine, Olivier, Pujol, Aurora, Peterson, Pärt, Mogensen, Trine H, Rowen, Lee, Mond, Jame, Debette, Stéphanie, de Lamballerie, Xavier, Burdet, Charle, Bouadma, Lila, Zins, Marie, Soler-Palacin, Pere, Colobran, Roger, Gorochov, Guy, Solanich, Xavier, Susen, Sophie, Martinez-Picado, Javier, Raoult, Didier, Vasse, Marc, Gregersen, Peter K, Piemonti, Lorenzo, Rodríguez-Gallego, Carlo, Notarangelo, Luigi D, Su, Helen C, Kisand, Kai, Okada, Satoshi, Puel, Anne, Jouanguy, Emmanuelle, Rice, Charles M, Tiberghien, Pierre, Zhang, Qian, Casanova, Jean-Laurent, Abel, Laurent, Cobat, Aurélie, Vougny, Marie-Christine, and Amsterdam Neuroscience - Neuroinfection & -inflammation
Subjects: Adult, Male, Risk, infection fatality rate, [SDV.IMM] Life Sciences [q-bio]/Immunology, autoantibodies, chronic mucocutaneous candidiasis, CHRONIC MUCOCUTANEOUS CANDIDIASIS, Autoimmunity, IMMUNITY, [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, Article, DISEASE, Basic medicine, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, INFECTION, Medicine and Health Sciences, Humans, IMUNOLOGIA, Aged, Autoantibodies, Aged, 80 and over, disease, [SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases, Multidisciplinary, SARS-CoV-2, type I IFNs, Age Factors, COVID-19, Type I IFNs, Middle Aged, autoantibodie, immunity, Antibodies, Neutralizing, infection, Infection fatality rate, Relative risk, relative risk, [SDV.TOX]Life Sciences [q-bio]/Toxicology, Interferon Type I, [SDV.IMM]Life Sciences [q-bio]/Immunology, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Female, type I IFN
Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection fatality rate (IFR) doubles with every 5 y of age from childhood onward. Circulating autoantibodies neutralizing IFN-α, IFN-ω, and/or IFN-β are found in ∼20% of deceased patients across age groups, and in ∼1% of individuals aged 4% of those >70 y old in the general population. With a sample of 1,261 unvaccinated deceased patients and 34,159 individuals of the general population sampled before the pandemic, we estimated both IFR and relative risk of death (RRD) across age groups for individuals carrying autoantibodies neutralizing type I IFNs, relative to noncarriers. The RRD associated with any combination of autoantibodies was higher in subjects under 70 y old. For autoantibodies neutralizing IFN-α2 or IFN-ω, the RRDs were 17.0 (95% CI: 11.7 to 24.7) and 5.8 (4.5 to 7.4) for individuals, The Laboratory of Human Genetics of Infectious Diseases is supported by the Howard Hughes Medical Institute; The Rockefeller University; the St. Giles Foundation; the NIH (Grants R01AI088364 and R01AI163029); the National Center for Advancing Translational Sciences; NIH Clinical and Translational Science Awards program (Grant UL1 TR001866); a Fast Grant from Emergent Ventures; Mercatus Center at George Mason University; the Yale Center for Mendelian Genomics and the Genome Sequencing Program Coordinating Center funded by the National Human Genome Research Institute (Grants UM1HG006504 and U24HG008956); the Yale High Performance Computing Center (Grant S10OD018521); the Fisher Center for Alzheimer’s Research Foundation; the Meyer Foundation; the JPB Foundation; the French National Research Agency (ANR) under the “Investments for the Future” program (Grant ANR-10-IAHU-01); the Integrative Biology of Emerging Infectious Diseases Laboratory of Excellence (Grant ANR-10-LABX-62-IBEID); the French Foundation for Medical Research (FRM) (Grant EQU201903007798); the French Agency for Research on AIDS and Viral hepatitis (ANRS) Nord-Sud (Grant ANRS-COV05); the ANR GENVIR (Grant ANR-20-CE93-003), AABIFNCOV (Grant ANR-20-CO11-0001), CNSVIRGEN (Grant ANR-19-CE15-0009-01), and GenMIS-C (Grant ANR-21-COVR-0039) projects; the Square Foundation; Grandir–Fonds de solidarité pour l’Enfance; the Fondation du Souffle; the SCOR Corporate Foundation for Science; The French Ministry of Higher Education, Research, and Innovation (Grant MESRI-COVID-19); Institut National de la Santé et de la Recherche Médicale (INSERM), REACTing-INSERM; and the University Paris Cité. P. Bastard was supported by the FRM (Award EA20170638020). P. Bastard., J.R., and T.L.V. were supported by the MD-PhD program of the Imagine Institute (with the support of Fondation Bettencourt Schueller). Work at the Neurometabolic Disease lab received funding from Centre for Biomedical Research on Rare Diseases (CIBERER) (Grant ACCI20-767) and the European Union's Horizon 2020 research and innovation program under grant agreement 824110 (EASI Genomics). Work in the Laboratory of Virology and Infectious Disease was supported by the NIH (Grants P01AI138398-S1, 2U19AI111825, and R01AI091707-10S1), a George Mason University Fast Grant, and the G. Harold and Leila Y. Mathers Charitable Foundation. The Infanta Leonor University Hospital supported the research of the Department of Internal Medicine and Allergology. The French COVID Cohort study group was sponsored by INSERM and supported by the REACTing consortium and by a grant from the French Ministry of Health (Grant PHRC 20-0424). The Cov-Contact Cohort was supported by the REACTing consortium, the French Ministry of Health, and the European Commission (Grant RECOVER WP 6). This work was also partly supported by the Intramural Research Program of the National Institute of Allergy and Infectious Diseases and the National Institute of Dental and Craniofacial Research, NIH (Grants ZIA AI001270 to L.D.N. and 1ZIAAI001265 to H.C.S.). This program is supported by the Agence Nationale de la Recherche (Grant ANR-10-LABX-69-01). K.K.’s group was supported by the Estonian Research Council, through Grants PRG117 and PRG377. R.H. was supported by an Al Jalila Foundation Seed Grant (Grant AJF202019), Dubai, United Arab Emirates, and a COVID-19 research grant (Grant CoV19-0307) from the University of Sharjah, United Arab Emirates. S.G.T. is supported by Investigator and Program Grants awarded by the National Health and Medical Research Council of Australia and a University of New South Wales COVID Rapid Response Initiative Grant. L.I. reports funding from Regione Lombardia, Italy (project “Risposta immune in pazienti con COVID-19 e co-morbidità”). This research was partially supported by the Instituto de Salud Carlos III (Grant COV20/0968). J.R.H. reports funding from Biomedical Advanced Research and Development Authority (Grant HHSO10201600031C). S.O. reports funding from Research Program on Emerging and Re-emerging Infectious Diseases from Japan Agency for Medical Research and Development (Grant JP20fk0108531). G.G. was supported by the ANR Flash COVID-19 program and SARS-CoV-2 Program of the Faculty of Medicine from Sorbonne University iCOVID programs. The 3C Study was conducted under a partnership agreement between INSERM, Victor Segalen Bordeaux 2 University, and Sanofi-Aventis. The Fondation pour la Recherche Médicale funded the preparation and initiation of the study. The 3C Study was also supported by the Caisse Nationale d’Assurance Maladie des Travailleurs Salariés, Direction générale de la Santé, Mutuelle Générale de l’Education Nationale, Institut de la Longévité, Conseils Régionaux of Aquitaine and Bourgogne, Fondation de France, and Ministry of Research–INSERM Program “Cohortes et collections de données biologiques.” S. Debette was supported by the University of Bordeaux Initiative of Excellence. P.K.G. reports funding from the National Cancer Institute, NIH, under Contract 75N91019D00024, Task Order 75N91021F00001. J.W. is supported by a Research Foundation - Flanders (FWO) Fundamental Clinical Mandate (Grant 1833317N). Sample processing at IrsiCaixa was possible thanks to the crowdfunding initiative YoMeCorono. Work at Vall d’Hebron was also partly supported by research funding from Instituto de Salud Carlos III Grant PI17/00660 cofinanced by the European Regional Development Fund (ERDF/FEDER). C.R.-G. and colleagues from the Canarian Health System Sequencing Hub were supported by the Instituto de Salud Carlos III (Grants COV20_01333 and COV20_01334), the Spanish Ministry for Science and Innovation (RTC-2017-6471-1; AEI/FEDER, European Union), Fundación DISA (Grants OA18/017 and OA20/024), and Cabildo Insular de Tenerife (Grants CGIEU0000219140 and “Apuestas científicas del ITER para colaborar en la lucha contra la COVID-19”). T.H.M. was supported by grants from the Novo Nordisk Foundation (Grants NNF20OC0064890 and NNF21OC0067157). C.M.B. is supported by a Michael Smith Foundation for Health Research Health Professional-Investigator Award. P.Q.H. and L. Hammarström were funded by the European Union’s Horizon 2020 research and innovation program (Antibody Therapy Against Coronavirus consortium, Grant 101003650). Work at Y.-L.L.’s laboratory in the University of Hong Kong (HKU) was supported by the Society for the Relief of Disabled Children. MBBS/PhD study of D.L. in HKU was supported by the Croucher Foundation. J.L.F. was supported in part by the Evaluation-Orientation de la Coopération Scientifique (ECOS) Nord - Coopération Scientifique France-Colombie (ECOS-Nord/Columbian Administrative department of Science, Technology and Innovation [COLCIENCIAS]/Colombian Ministry of National Education [MEN]/Colombian Institute of Educational Credit and Technical Studies Abroad [ICETEX, Grant 806-2018] and Colciencias Contract 713-2016 [Code 111574455633]). A. Klocperk was, in part, supported by Grants NU20-05-00282 and NV18-05-00162 issued by the Czech Health Research Council and Ministry of Health, Czech Republic. L.P. was funded by Program Project COVID-19 OSR-UniSR and Ministero della Salute (Grant COVID-2020-12371617). I.M. is a Senior Clinical Investigator at the Research Foundation–Flanders and is supported by the CSL Behring Chair of Primary Immunodeficiencies (PID); by the Katholieke Universiteit Leuven C1 Grant C16/18/007; by a Flanders Institute for Biotechnology-Grand Challenges - PID grant; by the FWO Grants G0C8517N, G0B5120N, and G0E8420N; and by the Jeffrey Modell Foundation. I.M. has received funding under the European Union’s Horizon 2020 research and innovation program (Grant Agreement 948959). E.A. received funding from the Hellenic Foundation for Research and Innovation (Grant INTERFLU 1574). M. Vidigal received funding from the São Paulo Research Foundation (Grant 2020/09702-1) and JBS SA (Grant 69004). The NH-COVAIR study group consortium was supported by a grant from the Meath Foundation.
Published: 2022
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98. Perceived social support and healthy eating self efficacy on the well-being of children and adolescents

Author: Villaecija, Joaquin, Luque, Barbara, Martinez, Sandra, Castillo-Mayen, Rosario, Cuadrado, Esther, Dominguez-Escribano, Marta, Tabernero, Carmen, [Villaecija, Joaquin] Univ Cordoba, Cordoba, Spain, [Luque, Barbara] Univ Cordoba, Cordoba, Spain, [Martinez, Sandra] Univ Cordoba, Cordoba, Spain, [Castillo-Mayen, Rosario] Univ Cordoba, Cordoba, Spain, [Cuadrado, Esther] Univ Cordoba, Cordoba, Spain, [Dominguez-Escribano, Marta] Univ Cordoba, Cordoba, Spain, [Villaecija, Joaquin] Inst Maimonides Invest Biomed Cordoba IMIBIC, Cordoba, Spain, [Luque, Barbara] Inst Maimonides Invest Biomed Cordoba IMIBIC, Cordoba, Spain, [Castillo-Mayen, Rosario] Inst Maimonides Invest Biomed Cordoba IMIBIC, Cordoba, Spain, [Cuadrado, Esther] Inst Maimonides Invest Biomed Cordoba IMIBIC, Cordoba, Spain, [Tabernero, Carmen] Univ Salamanca, Salamanca, Spain, [Tabernero, Carmen] Inst Neurociencias Castilla & Leon INCYL, Salamanca, Spain, Ministry of Science, Innovation and University of the Government of Spain, and Spanish Ministry of Science and Innovation
Subjects: Peer support, Predictors, Academic distress, Parent support, Body-mass index, Weight, Habits, Behaviors, Family, Quality-of-life, Obesity, Patterns, General Psychology, Body mass index
Abstract: Background: Unhealthy eating habits in children and adolescents and low personal judgment of efficacy in maintaining healthy eating behaviors have negative repercussions for health. These negative effects can have a differential affectation associated with psychosocial factors. Objectives/Method: The objectives were: to validate the Weight Efficacy Lifestyle (WEL) Questionnaire for Spanish children and adolescents; to analyze the relationship between well-being, socioeconomic level, body mass index, age, academic distress, social support for healthy and unhealthy eating, and self-efficacy; and to develop an explanatory model of well-being in children and adolescents based on their eating behaviors and other psychosocial behaviors. Results: Data were obtained from 299 children and adolescents (58.5% girls) aged from 9 to 18 years old (M-age = 12.92 years, SD = 2.74). Preliminary analysis showed adequate psychometric properties and results showed that perceived well-being was associated with lower academic distress and parent and peer social support for unhealthy eating, and with a better eating self-efficacy, parent support for healthy eating, and general weight management self-efficacy. Conclusions: Therefore, fostering confidence in children and adolescents about their weight management self-efficacy judgments may influence well-being, reduce body mass index, and prevent overweight and obesity.
Published: 2022

99. Prenatal exposure to mixtures of phthalates and phenols and body mass index and blood pressure in Spanish preadolescents

Author: Nuria Güil-Oumrait, German Cano-Sancho, Parisa Montazeri, Nikos Stratakis, Charline Warembourg, Maria-Jose Lopez-Espinosa, Jesús Vioque, Loreto Santa-Marina, Alba Jimeno-Romero, Rosa Ventura, Nuria Monfort, Martine Vrijheid, Maribel Casas, European Commission, Instituto de Salud Global - Institute For Global Health [Barcelona] (ISGlobal), Universitat Pompeu Fabra [Barcelona] (UPF), CIBER de Epidemiología y Salud Pública (CIBERESP), Laboratoire d'étude des Résidus et Contaminants dans les Aliments (LABERCA), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), École des Hautes Études en Santé Publique [EHESP] (EHESP), Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana [Espagne] (FISABIO), Universitat de València (UV), Universidad Miguel Hernández [Elche] (UMH), University of the Basque Country/Euskal Herriko Unibertsitatea (UPV/EHU), BIODonostia Research Institute, IMIM-Hospital del Mar, Generalitat de Catalunya, This study was funded by grants from Instituto de Salud Carlos III (Red INMA G03/176, CB06/02/0041, PI041436, PI081151 incl. FEDER funds), CIBERESP, Generalitat de Catalunya-CIRIT 1999SGR 00241, Generalitat de Catalunya-AGAUR 2009 SGR 501, Fundació La marató de TV3 (090430), EU Commission (261357, H2020 No 874583, the ATHLETE project, and No 825712, the OBERON project). Maribel Casas holds a Miguel Servet fellowship (MS16/00128) funded by Instituto de Salud Carlos III and co-funded by European Social Fund 'Investing in your future'. We acknowledge support from the Spanish Ministry of Science and Innovation and the State Research Agency through the 'Centro de Excelencia Severo Ochoa 2019-2023' Program (CEX2018-000806-S), and support from the Generalitat de Catalunya through the CERCA Program. INMA-Gipuzkoa: This study was funded by grants from Instituto de Salud Carlos III (FIS-PI13/02187 and FIS-PI18/01142 incl. FEDER funds), CIBERESP, Department of Health of the Basque Government (2015111065), and the Provincial Government of Gipuzkoa (DFG15/221) and annual agreements with the municipalities of the study area (Zumarraga, Urretxu, Legazpi, Azkoitia y Azpeitia y Beasain). INMA-Valencia: This study was funded by Grants from UE (FP7-ENV-2011 cod 282957 and HEALTH.2010.2.4.5-1), Spain: ISCIII (G03/176, FIS-FEDER: PI11/01007, PI11/02591, PI11/02038, PI12/00610, PI13/1944, PI13/2032, PI14/00891, PI14/01687, PI16/1288, and PI17/00663, and Miguel Servet-FEDER MS11/00178, MS15/00025, and MSII16/00051), Generalitat Valenciana (AICO/2021/182, and FISABIO: UGP 15-230, UGP-15-244, and UGP-15-249), and Alicia Koplowitz Foundation 2017.
Subjects: Pediatric Obesity, phthalates, blood pressure (BP), Phenol, [SDV]Life Sciences [q-bio], Phthalic Acids, Bayes Theorem, Blood Pressure, phenols, benzophenone-3, Body Mass Index, parabens, Cohort Studies, Pregnancy, Prenatal Exposure Delayed Effects, Humans, body mass index (BMI), Environmental Pollutants, Female, Child, General Environmental Science
Abstract: Background: Pregnant women are simultaneously exposed to several non-persistent endocrine-disrupting chem-icals, which may influence the risk of childhood obesity and cardiovascular diseases later in life. Previous prospective studies have mostly examined single-chemical effects, with inconsistent findings. We assessed the association between prenatal exposure to phthalates and phenols, individually and as a mixture, and body mass index (BMI) and blood pressure (BP) in preadolescents. Methods: We used data from the Spanish INMA birth cohort study (n = 1,015), where the 1st and 3rd-trimester maternal urinary concentrations of eight phthalate metabolites and six phenols were quantified. At 11 years of age, we calculated BMI z-scores and measured systolic and diastolic BP. We estimated individual chemical effects with linear mixed models and joint effects of the chemical mixture with hierarchical Bayesian kernel machine regression (BKMR). Analyses were stratified by sex and by puberty status. Results: In single-exposure models, benzophenone-3 (BP3) was nonmonotonically associated with higher BMI z -score (e.g. Quartile (Q) 3: beta = 0.23 [95% CI = 0.03, 0.44] vs Q1) and higher diastolic BP (Q2: beta = 1.27 [0.00, 2.53] mmHg vs Q1). Methyl paraben (MEPA) was associated with lower systolic BP (Q4: beta =-1.67 [-3.31,-0.04] mmHg vs Q1). No consistent associations were observed for the other compounds. Results from the BKMR confirmed the single-exposure results and showed similar patterns of associations, with BP3 having the highest importance in the mixture models, especially among preadolescents who reached puberty status. No overall mixture effect was found, except for a tendency of higher BMI z-score and lower systolic BP in girls. Conclusions: Prenatal exposure to UV-filter BP3 may be associated with higher BMI and diastolic BP during preadolescence, but there is little evidence for an overall phthalate and phenol mixture effect. We thank all study participants for their generous collaboration. INMA-Sabadell: This study was funded by grants from Instituto de Salud Carlos III (Red INMA G03/176; CB06/02/0041; PI041436; PI081151 incl. FEDER funds), CIBERESP, Generalitat de CatalunyaCIRIT 1999SGR 00241, Generalitat de Catalunya-AGAUR 2009 SGR 501, Fundacio La marato de TV3 (090430), EU Commission (261357, H2020 No 874583, the ATHLETE project, and No 825712, the OBERON project). Maribel Casas holds a Miguel Servet fellowship (MS16/00128) funded by Instituto de Salud Carlos III and co-funded by European Social Fund "Investing in your future". We acknowledge support from the Spanish Ministry of Science and Innovation and the State Research Agency through the "Centro de Excelencia Severo Ochoa 2019-2023" Program (CEX2018-000806-S), and support from the Generalitat de Catalunya through the CERCA Program. INMA-Gipuzkoa: This study was funded by grants from Instituto de Salud Carlos III (FIS-PI13/02187 and FIS-PI18/01142 incl. FEDER funds), CIBERESP, Department of Health of the Basque Government (2015111065), and the Provincial Government of Gipuzkoa (DFG15/221) and annual agreements with the municipalities of the study area (Zumarraga, Urretxu, Legazpi, Azkoitia y Azpeitia y Beasain). INMA-Valencia: This study was funded by Grants from UE (FP7-ENV-2011 cod 282957 and HEALTH.2010.2.4.5-1), Spain: ISCIII (G03/176; FIS-FEDER: PI11/01007, PI11/02591, PI11/02038, PI12/00610, PI13/1944, PI13/2032, PI14/00891, PI14/01687, PI16/1288, and PI17/00663; Miguel Servet-FEDER MS11/00178, MS15/00025, and MSII16/00051), Generalitat Valenciana (AICO/2021/182, and FISABIO: UGP 15-230, UGP-15-244, and UGP-15-249), and Alicia Koplowitz Foundation 2017.
Published: 2022
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100. Reaction of OH radicals with CH3NH2 in the gas phase: experimental (11.7-177.5 K) and computed rate coefficients (10-1000 K)

Author: Daniel González, Anxo Lema-Saavedra, Sara Espinosa, Emilio Martínez-Núñez, Antonio Fernández-Ramos, André Canosa, Bernabé Ballesteros, Elena Jiménez, Universidad de Castilla-La Mancha = University of Castilla-La Mancha (UCLM), Centro de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS ), Universidade de Santiago de Compostela [Spain] (USC ), Institut de Physique de Rennes (IPR), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Spanish Ministry of Science and Innovation (MICINN) through the CHEMLIFE project [PID2020-113936GB-I00], regional government of Castilla-La Mancha through the CINEMOL project [SBPLY/19/180501/000052], University of Castilla-La Mancha - UCLM [2021-GRIN-31279], UCLM (Plan Propio de Investigacion), CINEMOL project, Conselleria de Cultura, Educacion e Ordenacion Universitaria (Centro singular de investigacion de Galicia acreditacion 2019-2022) [ED431G 2019/03], Conselleria de Cultura, Educacion e Ordenacion Universitaria (Grupo de referencia competitiva) [ED431C 2021/40], European Regional Development Fund (ERDF), Ministerio de Ciencia e Innovacion [PID2019-107307RB-I00], and Xunta de Galicia
Subjects: CH3 NH2 en Fase Gas, [PHYS]Physics [physics], Reacción de Radicales OH, CH3 NH2 in the Gas Phase, General Physics and Astronomy, CH3NH2, Gas phase, Physical and Theoretical Chemistry, Fase gaseosa, CRESU, Molecular physics, Reaction of OH radicals
Abstract: Nitrogen-bearing molecules, like methylamine (CH3NH2), can be the building blocks of amino acids in the interstellar medium (ISM). At the ultralow temperatures of the ISM, it is important to know its gas-phase reactivity towards interstellar radicals and the products formed. In this work, the kinetics of the OH + CH3NH2 reaction was experimentally and theoretically investigated at low- and high-pressure limits (LPL and HPL) between 10 and 1000 K. Moreover, the CH2NH2 and CH3NH yields were computed in the same temperature range for both pressure regimes. A pulsed CRESU (French acronym for Reaction Kinetics in a Uniform Supersonic Flow) apparatus was employed to determine the rate coefficient, k(T), in the 11.7–177.5 K range. A drastic increase of k(T) when the temperature is lowered was observed in agreement with theoretical calculations, evaluated by the competitive canonical unified statistical (CCUS) theory, below 300 K in the LPL regime. The same trend was observed in the HPL regime below 350 K, but the theoretical k(T) values were higher than the experimental ones. Above 200 K, the calculated rate coefficients are improved with respect to previous computational studies and are in excellent agreement with the experimental literature data. In the LPL, the formation of CH3NH becomes largely dominant below ca. 100 K. Conversely, in the HPL regime, CH2NH2 is the only product below 100 K, whereas CH3NH becomes dominant at 298 K with a branching ratio similar to the one found in the LPL regime (≈70%). At T > 300 K, both reaction channels are competitive independently of the pressure regime, Las moléculas que contienen nitrógeno, como la metilamina (CH 3 NH 2 ), pueden ser los componentes básicos de los aminoácidos en el medio interestelar (ISM). A las temperaturas ultrabajas del ISM, es importante conocer su reactividad en fase gaseosa hacia los radicales interestelares y los productos formados. En este trabajo se investigó experimental y teóricamente la cinética de la reacción OH + CH 3 NH 2 a límites de baja y alta presión (LPL y HPL) entre 10 y 1000 K. Además, las reacciones CH 2 NH 2 y CH 3Los rendimientos de NH se calcularon en el mismo rango de temperatura para ambos regímenes de presión. Se empleó un aparato CRESU (acrónimo francés de Reaction Kinetics in a Uniform Supersonic Flow) pulsado para determinar el coeficiente de velocidad, k ( T ), en el rango de 11,7–177,5 K. De acuerdo con los cálculos teóricos, evaluados por la teoría de la estadística unificada canónica competitiva (CCUS), se observó un aumento drástico de k ( T ) cuando se baja la temperatura, por debajo de 300 K en el régimen LPL. La misma tendencia se observó en el régimen HPL por debajo de 350 K, pero el k teórico ( T) los valores fueron superiores a los experimentales. Por encima de 200 K, los coeficientes de velocidad calculados mejoran con respecto a estudios computacionales previos y están en excelente acuerdo con los datos de la literatura experimental. En el LPL, la formación de CH 3 NH se vuelve mayoritariamente dominante por debajo de ca. 100 K. Por el contrario, en el régimen HPL, CH 2 NH 2 es el único producto por debajo de 100 K, mientras que CH 3 NH se vuelve dominante a 298 K con una relación de ramificación similar a la encontrada en el régimen LPL (≈70%). A T > 300 K, ambos canales de reacción son competitivos independientemente del régimen de presión.
Published: 2022
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