244 results on '"Poetsch, M."'
Search Results
52. Clarification of dubious karyotypes in Hodgkin’s disease by simultaneous fluorescence immunophenotyping and interphase cytogenetics (FICTION)
- Author
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Weber-Matthiesen, K., primary, Deerberg, J., additional, Poetsch, M., additional, Grote, W., additional, and Schlegelberger, B., additional
- Published
- 1995
- Full Text
- View/download PDF
53. Down-regulation of the metastasis suppressor protein KAI1/CD82 correlates with occurrence of metastasis, prognosis and presence of HPV DNA in human penile squamous cell carcinoma.
- Author
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C. Protzel, B. Kleist, M. Poetsch, J. Giebel, Protzel, C, Kakies, C, Kleist, B, Poetsch, M, and Giebel, J
- Abstract
In penile squamous cell carcinoma (PSCC), the outcome largely depends on early detection and resection of inguinal lymph node metastases. We investigated the role of metastasis suppressor protein kang ai 1 (KAI1)/cluster of differentiation 82 (CD82), which is known to be of prognostic significance for a wide variety of cancers. Moreover, we analysed the tumours for human papillomavirus (HPV) DNA and loss of heterozygosity at the 11p11.2 locus. Tissue samples of 30 primary PSCCs were investigated immunohistochemically using an anti-KAI1/CD82 polyclonal antibody. The expression was assessed according to the degree of KAI1/CD82-positive tumour cells as positive, decreased or negative. The presence of HPV6/11, HPV16 and HPV18 DNA was analysed by polymerase chain reaction. All patients with decreased or negative expression of KAI1/CD82 in primary lesions had lymph node metastases (p = 0.0002). Patients with positive KAI1/CD82 expression showed a significant better prognosis for survival compared to the other groups (p = 0.0042). Presence of HPV DNA was associated with decreased or negative KAI1/CD82 expression. Lacking or decreased expression of metastasis suppressor gene KAI1/CD82 appears to be a prognostic parameter for the occurrence of lymph node metastases in PSCC. Our study suggests an association of decreased KAI1/CD82 expression with tumour progression, development of metastases and disease-specific death. [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
- View/download PDF
54. Different risk factors in basaloid and common squamous head and neck cancer.
- Author
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Kleist B, Bankau A, Lorenz G, Jäger B, and Poetsch M
- Published
- 2004
55. Intranodal palisaded myofibroblastoma with overexpression of cyclin d1.
- Author
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Kleist B, Poetsch M, and Schmoll J
- Abstract
Intranodal palisaded myofibroblastoma (IPM) is a rare benign spindle-cell tumor of lymph nodes with myofibroblastic/ smooth muscle differentiation. We present another case of IPM that confirms the myofibroblastic differentiation of the tumor cells and identifies the so-called amianthoid fibers as collagen deposits by immunohistochemical and ultrastructural techniques. Because IPM shares histomorphologic characteristics with an inflammatory myofibroblastic tumor that has been associated with a virus-induced alteration of cell cycle regulation, the diagnostic approach was extended in this case. We were able to demonstrate cyclin D1 overexpression but could detect neither amplification of the CCND1 gene nor allelic loss at chromosomes 9p22-21 and 13q (surrounding the genes p16 and Rb, respectively). Furthermore, no evidence of human herpesvirus-8 or Epstein-Barr virus infection could be found by polymerase chain reaction or immunostaining. Nevertheless, our results point to the cell cycle regulatory genes as a factor in the pathogenesis of IPM. [ABSTRACT FROM AUTHOR]
- Published
- 2003
- Full Text
- View/download PDF
56. Significance of the small subtelomeric area of chromosome 1 (1p36.3) in the progression of malignant melanoma: FISH deletion screening with YAC DNA probes.
- Author
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Poetsch, Micaela, Woenckhaus, Christian, Dittberner, Thomas, Pambor, Manfred, Lorenz, Gerd, Herrmann, Falko H., Poetsch, M, Woenckhaus, C, Dittberner, T, Pambor, M, Lorenz, G, and Herrmann, F H
- Abstract
The short arm of chromosome 1 (1p), especially the subtelomeric region of 1p36, is a common site for abnormalities in malignant melanoma of the skin. In a recent study nodular melanomas displayed deletions of 1p36 in an augmented percentage of cases. To evaluate the dimension of these deletions and to study their significance for the progression of malignant melanoma we analyzed seven melanoma cell lines, 32 primary tumors, and 32 metastatic tumors by fluorescence in situ hybridization with the DNA probe D1Z2 in 1p36.3 and eight YAC DNA probes hybridizing to 1p36, 1p32, 1p31, and 1p21. All cell lines, 91% of the metastatic tumors and 63% of nodular melanomas showed a deletion of 1p36.3. In the YAC hybridization experiments, the most frequent deletions were found in 1p36 in all cell lines, in 13% of nodular melanoma, and in 44% of metastatic tumors. Deletions in 1p36 were mostly confined to a rather small area near the locus D1Z2. The frequent occurrence of this deletion in melanomas with a high metastatic potential and the abundant accumulation of this deletion in metastasis point to genes located on 1p36, which might be of significance for the metastatic capability of malignant melanoma. [ABSTRACT FROM AUTHOR]
- Published
- 1999
- Full Text
- View/download PDF
57. Lhermitte-Duclos Disease in 3 Children: A Clinical Long-Term Observation
- Author
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Capone Mori, A., Hoeltzenbein, M., Poetsch, M., Schneider, J. F., Brandner, S., and Boltshauser, E.
- Published
- 2003
- Full Text
- View/download PDF
58. Analysis of microsatellite polymorphism in red deer, roe deer, and fallow deer - possible employment in forensic applications
- Author
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Poetsch, M., Seefeldt, S., Maschke, M., and Lignitz, E.
- Published
- 2001
- Full Text
- View/download PDF
59. PTEN/MMAC1 in malignant melanoma and its importance for tumor progression
- Author
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Poetsch, M., Dittberner, T., and Woenckhaus, C.
- Published
- 2001
- Full Text
- View/download PDF
60. Numerical Chromosome Aberrations Are Present Within the CD30+Hodgkin and Reed-Sternberg Cells in 100% of Analyzed Cases of Hodgkin's Disease
- Author
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Weber-Matthiesen, K., Deerberg, J., Poetsch, M., Grote, W., and Schlegelberger, B.
- Abstract
In Hodgkin's disease, cytogenetically aberrant clones have been demonstrated in a minority of cases studied. In the remaining cases, only normal metaphases have been found, but it is questionable whether normal karyotypes actually correspond to the pathognomonic Hodgkin and Reed-Sternberg (HRS) cells. Numerical aberrations could be studied by fluorescence in situ hybridization (FISH). However, in Hodgkin's disease, the percentage of tumor cells is mostly below the detection limit of FISH, which is near 1%. With the technique of simultaneous fluorescence immunophenotyping and interphase cytogenetic analysis (FICTION), this problem can be overcome. By FICTION, hybridization signals can selectively be evaluated within the CD30a+cell population. We have studied 30 cytogenetically analyzed cases of Hodgkin's disease by means of FICTION. In all cases, we found numerical chromosome aberrations within the majority of CD30+HRS cells. In cases with complex and hyperdiploid karyotypes, the cytogenetic results agreed with the FICTION data. There was considerable variability in the chromosome numbers, demonstrating that karyotype instability is an in vivo phenomenon of HRS cells. Lymphocytes never displayed numerical chromosome changes. Our results indicate that HRS cells regularly exhibit numerical chromosome aberrations and that the chromosome numbers are always in the hyper-ploid range.
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- 1995
- Full Text
- View/download PDF
61. An Increased Frequency of Numerical Chromosomal Abnormalities and 1p36 Deletions in Isolated Cells from Paraffin Sections of Malignant Melanomas by Means of Interphase Cytogenetics
- Author
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Poetsch, M., Woenckhaus, C., Dittberner, T., Pambor, M., Lorenz, G., and Herrmann, F. H.
- Published
- 1998
- Full Text
- View/download PDF
62. Quark charge retention in final state hadrons form deep inelastic muon scattering
- Author
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Albanese, J.P., Blum, D., Heusse, P., Jaffre, M., Jacholkowska, A., Pascaud, C., Bouard, X. De, Broll, C., Coignet, G., Eszes, G., Favier, J., Jancso, G., Maire, M., Nagy, E., Pessard, H., Ribarics, P., Schneegans, M., Thenard, J.M., Toth, J., Urban, L., Brasse, F.W., Flauger, W., Gayler, J., Goessling, C., Korbel, V., Nassalski, J., Poensgen, B., Zank, P., Carr, J., Chima, J.S., Clifft, R., Edwards, M., Norton, P.R., Oakham, F.G., Thompson, J.C., Dobinson, R.W., Dosseli, U., Gabathuler, E., Kellner, G., Montgomery, H.E., Osborne, A.M., Figiel, J., Hoppe, C., Janata, F., Preissner, H., Rondio, E., Studt, M., La Torre, A. De, Dengler, F., Derado, I., Eckardt, V., Manz, A., Schmitz, N., Shiers, J., Wolf, G., Arneodo, M., Arvidson, A., Aubert, J.J., Becks, K.H., Bee, C., Benchouk, C., Bird, I., Boehm, E., Braun, H., Brown, S., Brueck, H., Calen, H., Callebaut, D., Cobb, J.H., Combley, F., Coughlan, J., Court, G.R., d'Agostini, G., Dahlgren, S., Davies, J.K., Dau, W.D., Dreyer, T., Drees, J., Dumont, J.J., Dueren, M., Edwards, A., Ernst, T., Ferrero, M.I., Foster, J., Gamet, R., Geddes, N., Giubellino, P., Grafstroem, P., Grard, F., Gustafsson, L., Haas, J., Hagberg, E., Hasert, F.J., Hayman, P., Johnson, A.S., Kabuss, E.M., Krueger, J., Kullander, S., Landgraf, U., Lanske, D., Loken, J., Long, K., Mohr, W., Montanet, F., Mount, R.P., Paul, L., Payre, P., Peroni, C., Pettingale, J., Poetsch, M., Renton, P., Rith, K., Schlagboehmer, A., Schroeder, T., Schultze, K., Sloan, T., Stier, H.E., Stockhausen, W., Taylor, G., Wahlen, H., Wallucks, W., Whalley, M., Williams, W.S.C., Wheeler, S., Wimpenny, S., Windmolders, R., Centre de Physique des Particules de Marseille (CPPM), Aix Marseille Université (AMU)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de l'Accélérateur Linéaire (LAL), Université Paris-Sud - Paris 11 (UP11)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Paris-Sud - Paris 11 (UP11), and Starita, Sabine
- Subjects
[PHYS.HEXP] Physics [physics]/High Energy Physics - Experiment [hep-ex] ,[PHYS.HEXP]Physics [physics]/High Energy Physics - Experiment [hep-ex] - Published
- 1984
63. The vertex and large angle detectors of a spectrometer system for high energy muon physics
- Author
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Davis, A., Dobinson, R.W., Dosselli, U., Edwards, A., Gabathuler, E., Kellner, G., Montgomery, H.E., Mueller, H., Osborne, A.M., Scaramelli, A., Watson, E., Brasse, F.W., Falley, G., Flauger, W., Gayler, J., Goessling, C., Koll, J., Korbel, V., Nassalski, J., Singer, G., Thiele, K., Zank, P., Figiel, J., Janata, F., Rondio, E., Studt, M., Torre, A. De La, Bernaudin, B., Blum, D., Heusse, P., Jaffre, M., Noppe, J.M., Pascaud, C., Bertsch, Y., Bouard, X. De, Broll, C., Coignet, G., Favier, J., Jansco, G., Lebeau, M., Maire, M., Minssieux, H., Montanet, F., Moynot, M., Nagy, E., Payre, P., Perrot, G., Pessard, H., Ribarics, P., Schneegans, M., Thenard, J.M., Botterill, D., Carr, J., Clifft, R., Edwards, M., Norton, P.R., Rousseau, M.D., Sproston, M., Thompson, J.C., Albanese, J.P., Allkofer, O.C., Arneodo, M., Aubert, J.J., Becks, K.H., Bee, C., Benchouk, C., Bianchi, F., Bibby, J., Bird, I., Boehm, E., Braun, H., Brown, S., Brueck, H., Callebaut, D., Cobb, J.H., Combley, F., Cornelssen, M., Costa, F., Coughlan, J., Court, G.R., D'Agostini, G., Dau, W.D., Davies, J.K., Dengler, F., Derado, I., Drees, J., Dumont, J.J., Eckardt, V., Ferrero, M.I., Gamet, R., Gebauer, H.J., Haas, J., Hasert, F.J., Hayman, P., Johnson, A.S., Kabuss, E.M., Kahl, T., Krueger, J., Landgraf, U., Lanske, D., Loken, J., Manz, A., Mermet-Guyennet, M., Mohr, W., Moser, K., Mount, R.P., Paul, L., Peroni, C., Pettingale, J., Poetsch, M., Preissner, H., Renton, P., Rith, K., Roehner, F., Schlagboehmer, A., Schmitz, N., Schultze, K., Shiers, J., Sloan, T., Smith, R., Stier, H.E., Stockhausen, W., Wahlen, H., Wallucks, W., Whalley, M., Williams, D.A., Williams, W.S.C., Wimpenny, S., Windmolders, R., Winkmueller, G., Wolf, G., Laboratoire de l'Accélérateur Linéaire (LAL), Université Paris-Sud - Paris 11 (UP11)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Centre de Physique des Particules de Marseille (CPPM), Aix Marseille Université (AMU)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), and Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Paris-Sud - Paris 11 (UP11)
- Subjects
[PHYS.PHYS.PHYS-INS-DET]Physics [physics]/Physics [physics]/Instrumentation and Detectors [physics.ins-det] - Published
- 1983
64. Quark and diquark fragmentation into neutral strange particles as observed in muon-proton interactions at 280 GeV
- Author
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Arneodo, M., Blum, D., Heusse, P., Jaffre, M., Jacholkowska, A., Pascaud, C., Bouard, X. De, Broll, C., Coignet, G., Eszes, G., Favier, J., Jansco, G., Maire, M., Nagy, E., Osbourne, A.M., Pessard, H., Ribarics, P., Schneegans, M., Thenard, J.M., Toth, J., Urban, L., Flauger, W., Gayler, J., Goessling, C., Korbel, V., Nassalski, J., Poensgen, B., Zank, B., Carr, J., Chima, J.S., Clifft, R., Edwards, M., Norton, P.R., Oakham, F.G., Thompson, J.C., Dosseli, V., Gabathuler, E., Haas, J., Kellner, G., Montgomery, H.E., Figiel, J., Hoppe, C., Janata, F., Rondio, E., Studt, M., Torre, A. de La, Arvidson, A., Aubert, J.J., Beaufays, J., Becks, K.H., Bee, C., Benchouk, C., Bird, I., Boehm, E., Braun, H., Brown, S., Brueck, H., Calen, H., Callebaut, D., Cobb, J.H., Combley, F., Coughlan, J., Court, G.R., d'Agostini, G., Dahlgren, S., Davies, J.K., Dau, W.D., Dengle, R.F., Derado, I., Dreyer, T., Drees, J., Dumont, J.J., Dueren, M., Eckhart, V., Edwards, A., Ernst, T., Ferrero, M.I., Foster, J., Gamet, R., Geddes, N., Giubellino, P., Grafstroem, P., Grard, F., Gustaffson, L., Hagberg, E., Hasert, F.J., Haymann, Jean-Philippe, Johnson, A.S., Kabuss, E.M., Krueger, J., Kullander, S., Landgraf, U., Lanske, D., Loken, J., Long, K., Manz, A., Mohr, W., Montanet, F., Mount, R.P., Paul, L., Payre, P., Peroni, C., Pettingale, J., Pietrzyk, B., Poetsch, M., Preissner, H., Renton, P., Rith, K., Schlagboehmer, A., Schmitz, N., Schroeder, T., Schultze, K., Shiers, J., Sloan, T., Stier, H.E., Stockhausen, W., Taylor, G.N., Wahlen, H., Wallucks, W., Whalley, M., Wheeler, S., Williams, W.S.C., Williamson, J., Wimpenny, S., Windmolders, R., Wittek, W., Wolf, G., Laboratoire de l'Accélérateur Linéaire (LAL), Université Paris-Sud - Paris 11 (UP11)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Annecy de Physique des Particules (LAPP), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS), Centre de Physique des Particules de Marseille (CPPM), Aix Marseille Université (AMU)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), EMC, and Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Paris-Sud - Paris 11 (UP11)
- Subjects
[PHYS.HEXP]Physics [physics]/High Energy Physics - Experiment [hep-ex] - Published
- 1984
65. Investigation of the W and Q2 dependence of charged pion distributions in $\mu$p scattering
- Author
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Arneodo, M., Ferrero, M.I., Giubellino, P., Peroni, C., Arvidson, A., Badelek, B., Calen, H., Dahlgren, S., Grafstroem, P., Hagberg, E., Kullander, S., Aubert, J.J., Benchouk, C., D'Agostini, G., Montanet, F., Payre, P., Pietrzyk, B., Beaufays, J., Kellner, G., Montgomery, H.E., Osborne, A.M., Chima, J.S., Clifft, R., Norten, P.R., Oakham, F.G., Thompson, J.C., Berghoff, G., Dueren, M., Hasert, F.J., Lanske, D., Scheer, M., Scholz, M., Schultze, K., Bee, C.P., Bird, I., Blum, D., Coughlan, J., Sloan, T., Heusse, P., Jaffre, M., Jacholkowska, A., Pascaud, C., Boehm, E., Bouard, X. De, Broll, C., Coignet, G., Eszes, G., Favier, J., Maire, M., Nagy, E., Pessard, H., Ribarics, P., Schneegans, M., Thenard, J.M., Toth, J., Urban, L., Brasse, F.W., Flauger, W., Gayler, J., Korbel, V., Nassalski, J., Braun, H., Brueck, H., Drees, J., Edwards, A., Krueger, J., Poetsch, M., Brown, S., Gabathuler, E., Gamet, R., Hayman, P., Pettingale, J., Wimpenny, S.J., Ciborowski, J., Fiegiel, J., Gajewski, J., Hoppe, C., Janata, F., Poensgen, B., Rondio, E., Schiemann, H., Studt, M., Torre, A. De La, Combley, F., Foster, J., Whalley, M., Wheeler, S., Dengler, F., Derado, I., Jansco, G., Malecki, P., Manz, A., Maselli, S., Eckhardt, V., Pawlik, B., Schmitz, N., Schouten, M., Wolf, G., Dreyer, T., Ernst, T., Haas, J., Kabuss, E.M., Centre de Physique des Particules de Marseille (CPPM), Aix Marseille Université (AMU)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de l'Accélérateur Linéaire (LAL), Université Paris-Sud - Paris 11 (UP11)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), and Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Paris-Sud - Paris 11 (UP11)
- Subjects
[PHYS.HEXP]Physics [physics]/High Energy Physics - Experiment [hep-ex] - Published
- 1986
66. Studies of quark and diquark fragmentation into identified hadrons in deep inelastic muon-proton scattering
- Author
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Arneodo, M., Ferrero, M.I., Giubellino, P., Peroni, C., Blum, D., Heusse, P., Jaffre, M., Jacholskowska, A., Pascaud, C., Bouard, X. De, Broll, C., Coignet, G., Eszes, G., Favier, J., Jancso, G., Maire, M., Montanet, F., Nagy, E., Pessard, H., Ribarics, P., Schneegans, M., Thenard, J.M., Toth, J., Brasse, F.W., Flauger, W., Gajewski, J., Gayler, J., Goessling, C., Korbel, V., Nassalski, J., Poensgen, B., Carr, J., Chima, J.S., Clifft, R., Edwards, M., Norton, P.R., Oakham, F.G., Thompson, J.C., Ciborowski, J., Figiel, J., Hoppe, C., Janata, F., Rondio, E., Schiemann, H., Studt, M., De La Torre, A., Dosselli, U., Gabathuler, E., Haas, J., Kellner, G., Montgomery, H.E., Osborne, A.M., Arvidson, A., Aubert, J.J., Badelek, B., Beaufays, J., Becks, K.H., Bee, C., Benchouk, C., Berghoff, G., Bird, I., Boehm, E., Braun, H., Brown, S., Brueck, H., Calen, H., Callebaut, D., Cobb, J.H., Combley, F., Coughlan, J., Court, G.R., Dahlgren, S., Davies, J.K., Dengler, F., Derado, I., Dreyer, T., Drees, J., Dumont, J.J., Dueren, M., Eckardt, V., Ernst, T., Foster, J., Gamet, R., Geddes, N., Grafstroem, P., Grard, F., Gustafsson, L., Hagberg, E., Hasert, F.J., Hayman, P., Johnson, A.S., Kabuss, E.M., Krueger, J., Kullander, S., Landgraf, U., Lanske, D., Loken, J., Long, K., Manz, A., Mohr, W., Mount, R.P., Paul, L., Pawlik, B., Payre, P., Pettingale, J., Pietrzyk, B., Poetsch, M., Preissner, H., Renton, P., Rith, K., Schlagboehmer, A., Schmitz, N., Schroeder, T., Schultze, K., Shiers, J., Sloan, T., Stier, H.E., Stockhausen, W., Taylor, G.N., Urban, L., Wahlen, H., Wallucks, W., Whalley, M., Wheeler, S., Williams, W.S.C., Wimpenny, S., Windmolders, R., Wolf, G., Laboratoire de l'Accélérateur Linéaire (LAL), Université Paris-Sud - Paris 11 (UP11)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Annecy de Physique des Particules (LAPP), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS), Centre de Physique des Particules de Marseille (CPPM), Aix Marseille Université (AMU)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), EMC, and Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Paris-Sud - Paris 11 (UP11)
- Subjects
[PHYS.HEXP]Physics [physics]/High Energy Physics - Experiment [hep-ex] - Published
- 1985
67. Charge and transverse momentum correlations in deep inelastic muon-proton scattering
- Author
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Arneodo, M., Ferrero, M.I., Maselli, S., Peroni, C., Arvidson, A., Badelek, B., Calen, H., Dahlgren, S., Grafstroem, P., Hagberg, E., Kullander, S., Aubert, J.J., Benchouk, C., d'Agostini, G., Montanet, F., Payre, P., Pietrzyk, B., Beaufays, J., Kellner, G., Osborne, A.M., Bee, C., Chima, J.S., Clifft, R., Edwards, M., Norton, P.R., Oakham, F.G., Thompson, J.C., Berghoff, G., Dueren, M., Hasert, F.J., Lanske, D., Scheer, M., Scholz, M., Schultze, K., Bird, I., Sloan, T., Blum, D., Heusse, P., Jaffre, M., Jacholkowska, A., Pascaud, C., Boehm, E., Coughlan, J., Bouard, X. De, Broll, C., Coignet, G., Eszes, G., Favier, J., Maire, M., Nagy, E., Pessard, H., Ribarics, P., Schneegans, M., Thenard, J.M., Toth, J., Urban, L., Brasse, F.W., Flauger, W., Gayler, J., Korbel, V., Nassalski, J., Braun, H., Brueck, H., Drees, J., Edwards, A., Krueger, J., Poetsch, M., Brown, S., Gabathuler, E., Gamet, R., Hayman, P., Pettingale, J., Wimpenny, S., Ciborowski, J., Figiel, J., Gajewski, J., Janata, F., Poensgen, B., Rondio, E., Schiemann, H., Studt, M., Torre, A. de La, Combley, F., Foster, J., Whalley, M., Wheeler, S., Dengler, F., Derado, I., Jancso, G., Malecki, P., Manz, A., Eckardt, V., Pawlik, B., Schmitz, N., Schouten, M., Wolf, G., Dreyer, T., Ernst, T., Haas, J., Kabuss, E.M., Landgraf, U., Mohr, W., Rith, K., Starita, Sabine, Centre de Physique des Particules de Marseille (CPPM), Aix Marseille Université (AMU)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de l'Accélérateur Linéaire (LAL), Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Paris-Sud - Paris 11 (UP11), and Université Paris-Sud - Paris 11 (UP11)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[PHYS.HEXP] Physics [physics]/High Energy Physics - Experiment [hep-ex] ,[PHYS.HEXP]Physics [physics]/High Energy Physics - Experiment [hep-ex] - Published
- 1986
68. Transverse momentum and its compensation in current and target jets in deep inelastic muon-proton scattering
- Author
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Arneodo, M., Giubellino, P., Peroni, C., Dosseli, U., Haas, J., Kellner, G., Montgomery, H.E., Osborne, A.M., Brasse, F.W., Flauger, W., Goessling, C., Korbel, V., Nassalski, J., Fiegiel, J., Hoppe, C., Janata, F., Rondio, E., Studt, M., Torre, A. De La, Blum, D., Heusse, P., Jaffre, M., Jacholkowska, A., Pascaud, C., Bouard, X. De, Broll, C., Coignet, G., Eszes, G., Favier, J., Jancso, G., Maire, M., Nagy, E., Pessard, H., Ribarics, P., Schneegans, M., Thenard, J.M., Toth, J., Urban, L., Dengler, F., Derado, I., Eckardt, V., Manz, A., Pawlik, B., Schmitz, N., Shiers, J., Wolf, G., Carr, J. Chima J.S., Clifft, R., Edwards, M., Norton, P.R., Oakham, F.G., Thompson, J.C., Arvidson, A., Aubert, J.J., Beaufays, J., Becks, K.H., Bee, C., Benchouk, C., Bird, I., Boehm, E., Braun, H., Brown, S., Brueck, H., Calen, H., Callebaut, D., Cobb, J.H., Combley, F., Coughlan, J., Court, G.R., D'Agostini, G., Dahlgren, S., Davies, J.K., Drees, J., Dumont, J.J., Dueren, M., Edwards, A., Ferrerro, M.I., Foster, J., Gabathuler, E., Gamet, R., Geddes, N., Grafstroem, P., Grard, F., Gustafsson, L., Hagberg, E., Hasert, F.J., Hayman, P., Johnson, A.S., Krueger, J., Kullander, S., Lanske, D., Loken, J., Long, K., Montanet, F., Mount, R.P., Paul, L., Payre, P., Pettingale, J., Pietrzyk, B., Poetsch, M., Preissner, H., Renton, P., Schultze, K., Sloan, T., Stockhausen, W., Taylor, G.N., Wahlen, H., Whalley, M., Wheeler, S., Williams, W.S.C., Wimpenny, S., Windmolders, R., Starita, Sabine, Laboratoire de l'Accélérateur Linéaire (LAL), Université Paris-Sud - Paris 11 (UP11)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Centre de Physique des Particules de Marseille (CPPM), Aix Marseille Université (AMU)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), and Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Paris-Sud - Paris 11 (UP11)
- Subjects
[PHYS.HEXP] Physics [physics]/High Energy Physics - Experiment [hep-ex] ,[PHYS.HEXP]Physics [physics]/High Energy Physics - Experiment [hep-ex] - Published
- 1984
69. Clarification of dubious karyotypes in Hodgkin's disease by simultaneous fluorescence immunophenotyping and interphase cytogenetics (FICTION).
- Author
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Weber-Matthiesen, K., Deerberg, J., Poetsch, M., Grote, W., and Schlegelberger, B.
- Published
- 1995
- Full Text
- View/download PDF
70. ChemInform Abstract: AMICLENOMYCIN PEPTIDES - ISOLATION AND STRUCTURE ELUCIDATION OF NEW BIOTIN ANTIMETABOLITES
- Author
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KERN, A., primary, KABATEK, U., additional, JUNG, G., additional, WERNER, R. G., additional, POETSCH, M., additional, and ZAEHNER, H., additional
- Published
- 1985
- Full Text
- View/download PDF
71. Does the PITSLRE gene complex contribute to the pathogenesis of malignant melanoma of the skin? A study of patient-derived tumor samples
- Author
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Poetsch, M., Dittberner, T., and Woenckhaus, C.
- Published
- 2001
- Full Text
- View/download PDF
72. Measurements of the nucleon structure function in the range 0.002 < x < 0.17 and 00.2 < Q2 < 8 GeV2 in deuterium, carbon and calcium
- Author
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Arneodo, M., Arvidson, A., Aubert, J.J., Badelek, B., Beaufays, J., Bee, C.P., Benchouk, C., Berghoff, G., Bird, I.G., Blum, D., Böhm, E., de Bouard, X., Brasse, F.W., Braun, H., Broll, C., Brown, S.C., Brück, H., Calén, H., Chima, J.S., Ciborowski, J., Clifft, R., Coignet, G., Combley, F., Coughlan, J., d'Agostini, G., Dahlgren, S., Derado, I., Dreyer, T., Drees, J., Düren, M., Eckardt, V., Edwards, A., Edwards, M., Ernst, T., Eszes, G., Favier, J., Ferrero, M.I., Figiel, J., Flauger, W., Foster, J., Gabathuler, E., Gajewski, J., Gamet, R., Geddes, N., Grafström, P., Gustafsson, L., Haas, J., Hagberg, E., Hasert, F.J., Hayman, P., Heusse, P., Jaffre, M., Jacholkowska, A., Janata, F., Jancso, G., Johnson, A.S., Kabuss, E.M., Kellner, G., Krüger, A., Krüger, J., Kullander, S., Landgraf, U., Lanske, D., Loken, J., Long, K., Maire, M., Malecki, P., Manz, A., Maselli, S., Mohr, W., Montanet, F., Montgomery, H.E., Nagy, E., Nassalski, J., Norton, P.R., Oakham, F.G., Osborne, A.M., Pascaud, C., Pawlik, B., Payre, P., Peroni, C., Peschel, H., Pessard, H., Pettingale, J., Pietrzyk, B., Poensgen, B., Pötsch, M., Renton, P., Ribarics, P., Rith, K., Rondio, E., Sandacz, A., Scheer, M., Schlagböhmer, A., Schiemann, H., Schmitz, N., Schneegans, M., Scholz, M., Schouten, M., Schröder, T., Schultze, K., Sloan, T., Stier, H.E., Studt, M., Taylor, G.N., Thenard, J.M., Thompson, J.C., de la Torre, A., Toth, J., Urban, L., Wallucks, W., Whalley, M., Wheeler, S., Williams, W.S.C., Wimpenny, S.J., Windmolders, R., and Wolf, G.
- Published
- 1990
- Full Text
- View/download PDF
73. Measurements of the u valence quark distribution function in the proton and u quark fragmentation functions
- Author
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Arneodo, M., Arvidson, A., Aubert, J.J., Badelek, B., Beaufays, J., Bee, C.P., Benchouk, C., Berghoff, G., Bird, I.G., Blum, D., Böhm, E., De Bouard, X., Brasse, F.W., Braun, H., Broll, C., Brown, S.C., Brück, H., Calen, H., Chima, J.S., Ciborowski, J., Clifft, R., Coignet, G., Combley, F., Coughlan, J., D'Agostini, G., Dahlgren, S., Dengler, F., Derado, I., Dreyer, T., Drees, J., Düren, M., Eckardt, V., Edwards, A., Edwards, M., Ernst, T., Eszes, G., Favier, J., Ferrero, M.I., Figiel, J., Flauger, W., Foster, J., Gabathuler, E., Gajewski, J., Gamet, R., Gayler, J., Geddes, N., Grafström, P., Grard, F., Haas, J., Hagberg, E., Hasert, F.J., Hayman, P., Heusse, P., Jaffre, M., Jacholkowska, A., Janata, F., Jancso, G., Johnson, A.S., Kabuss, E.M., Kellner, G., Korbel, V., Krüger, A., Krüger, J., Kullander, S., Landgraf, U., Lanske, D., Loken, J., Long, K., Maire, M., Malecki, P., Manz, A., Maselli, S., Mohr, W., Montanet, F., Montgomery, H.E., Nagy, E., Nassalski, J., Norton, P.R., Oakham, F.G., Osborne, A.M., Pascaud, C., Pawlik, B., Payre, P., Peroni, C., Peschel, H., Pessard, H., Pettingale, J., Pietrzyk, B., Poensgen, B., Pötsch, M., Renton, P., Ribarics, P., Rith, K., Rondio, E., Sandacz, A., Scheer, M., Schlagböhmer, A., Schiemann, H., Schmitz, N., Schneegans, M., Scholz, M., Schouten, M., Schröder, T., Schultze, K., Sloan, T., Stier, H.E., Studt, M., Taylor, G.N., Thenard, J.M., Thompson, J.C., De la Torre, A., Toth, J., Urban, L., Wallucks, W., Whalley, M., Wheeler, S., Williams, W.S.C., Wimpenny, S.J., Windmolders, R., and Wolf, G.
- Published
- 1989
- Full Text
- View/download PDF
74. Shadowing in deep inelastic muon scattering from nuclear targets
- Author
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Arneodo, M., Arvidson, A., Aubert, J.J., Badelek, B., Beaufays, J., Bee, C.P., Benchouk, C., Berghoff, G., Bird, I., Blum, D., Böhm, E., De Bouard, X., Brasse, F.W., Braun, H., Broll, C., Brown, S., Brück, H., Brüll, A., Calen, H., Chima, J.S., Ciborowski, J., Clifft, R., Coignet, G., Combley, F., Coughlan, J., D'Agostini, G., Dahlgren, S., Dengler, F., Derado, I., Dreyer, T., Drees, J., Drobnitzki, M., Düren, M., Eckardt, V., Edwards, A., Edwards, M., Ernst, T., Eszes, G., Favier, J., Ferrero, M.I., Figiel, J., Foster, J., Ftacnik, J., Gabathuler, E., Gajewski, J., Gamet, R., Geddes, N., Grafström, P., Gustafsson, L., Haas, J., Hagberg, E., Hasert, F.J., Hayman, P., Heusse, P., Jaffré, M., Jacholkowska, A., Janata, F., Jancso, G., Johnson, A.S., Kabuss, E.M., Kaiser, R., Kellner, G., Krüger, A., Krüger, J., Kullander, S., Landgraf, U., Lanske, D., Loken, J., Long, K., Maire, M., Malecki, P., Manz, A., Maselli, S., Mohr, W., Montanet, F., Montgomery, H.E., Nagy, E., Nassalski, J., Norton, P.R., Oakham, F.G., Osborne, A.M., Pascaud, C., Pawlik, B., Payre, P., Peroni, C., Peschel, H., Pessard, H., Pettingale, J., Pietrzyk, B., Pietrzyk, U., Pönsgen, B., Pötsch, M., Renton, P., Ribarics, P., Rith, K., Rondio, E., Sandacz, A., Scheer, M., Schlagböhmer, A., Schiemann, H., Schmitz, N., Schneegans, M., Scholz, M., Schröder, T., Schultze, K., Seidel, A., Sloan, T., Stier, H.E., Studt, M., Taylor, G.N., Thénard, J.M., Thompson, J.C., De La Torre, A., Toth, J., Urban, L., Wallucks, W., Whalley, M., Wheeler, S., Williams, W.S.C., Wimpenny, S.J., Windmolders, R., Wolf, G., and Ziemons, K.
- Published
- 1988
- Full Text
- View/download PDF
75. Allelverteilung in X-chromosomalen Short-Tandem-Repeats bei Afrikanern : Daten aus Ghana und Marokko
- Author
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Stallmann, Daniela and Poetsch, M. (Akademische Betreuung)
- Subjects
Medizin - Abstract
Duisburg, Essen, Univ., Diss., 2012
- Published
- 2011
76. (Un)Reliable detection of menstrual blood in forensic casework - evaluation of the Seratec® PMB test with mock samples.
- Author
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Konrad H, Hartung B, and Poetsch M
- Subjects
- Humans, Male, Saliva chemistry, Bodily Secretions chemistry, Hemoglobins analysis, Forensic Genetics methods, Semen chemistry, Body Fluids chemistry
- Abstract
The identification of the type of body fluid in crime scene evidence may be crucial, so that the efforts are high to reduce the complexity of these analyses and to minimize time and costs. Reliable immunochromatographic rapid tests for specific and sensitive identification of blood, saliva, urine and sperm secretions are already routinely used in forensic genetics. The recently introduced Seratec® PMB test is said to detect not only hemoglobin, but also differentiate menstrual blood from other secretions containing blood (cells) by detecting D-dimers. In our experimental set-up, menstrual blood could be reliably detected in mock forensic samples. Here, the result was independent of sample age and extraction buffer volume. It was also successfully demonstrated that all secretions without blood cells were negative for both, hemoglobin (P) and D-dimer (M). However, several blood cell-containing secretions/tissues comprising blood (injury), nasal blood, postmortem blood and wound crust also demonstrated positive results for D-dimer (M) and were therefore false positives. For blood (injury) and nasal blood, this result was reproduced for different extraction buffer volumes. The results of this study clearly demonstrate that the Seratec® PMB test is neither useful nor suitable for use in forensic genetics because of the great risk of false positive results which can lead to false conclusions, especially in sexual offense or violent acts., (© 2023. The Author(s).)
- Published
- 2024
- Full Text
- View/download PDF
77. More than just blood, saliva, or sperm-setup of a workflow for body fluid identification by DNA methylation analysis.
- Author
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Konrad H, Jürgens L, Hartung B, and Poetsch M
- Subjects
- Humans, Male, Female, Semen chemistry, Workflow, Saliva chemistry, Body Fluids chemistry, Menstruation, Spermatozoa chemistry, Blood Chemical Analysis, Genetic Markers, DNA Fingerprinting methods, DNA Methylation, Cervix Mucus chemistry, CpG Islands, Forensic Genetics methods
- Abstract
The determination of cellular origin of DNA is a useful method in forensic genetics and complements identification of the DNA donor by STR analysis, since it could provide helpful information for the reconstruction of crime scenes and verify or disprove the descriptions of involved people. There already exist several rapid/pre-tests for several secretions (blood, sperm secretion, saliva, and urine), RNA-based expression analyses (blood, menstrual blood, saliva, vaginal secretion, nasal secretion, and sperm secretion), or specific CpG methylation analyses (nasal blood, blood, saliva, vaginal secretion, nasal secretion, and sperm secretion) for determining the cell type.To identify and to discriminate seven different body fluids and mixtures thereof in a simple workflow from each other, assays based on specific methylation patterns at several CpGs combined with pre-/rapid tests were set up in this study. For each of the seven secretions listed above, we selected the CpG marker achieving the highest possible discrimination (out of 30 markers tested). Validation studies confirmed a definite identification for saliva, vaginal secretion, and semen secretion in 100% of samples as well as discrimination from all other secretions. Moreover, the unambiguously correctly determined proportion of nasal samples, blood and menstrual blood varied between 61% (nasal blood) and 85% (nasal secretion).In summary, our workflow proved to be an easy and useful tool in forensic analysis for the identification and discrimination of seven different body fluids often found at a crime scene., (© 2023. The Author(s).)
- Published
- 2023
- Full Text
- View/download PDF
78. Knife wound or nosebleed-where does the blood at the crime scene come from?
- Author
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Konrad H, Lawniczek J, Bajramjan C, Weber L, Bajanowski T, and Poetsch M
- Subjects
- Female, Humans, Forensic Genetics, Saliva chemistry, Semen chemistry, DNA analysis, Crime, Epistaxis genetics, DNA Methylation
- Abstract
Secretion analysis is a useful tool in forensic genetics, since it establishes the (cellular) origin of the DNA prior in addition to the identification of the DNA donor. This information can be crucial for the construction of the crime sequence or verification of statements of people involved in the crime. For some secretions, rapid/pretests already exist (blood, semen, urine, and saliva) or can be determined via published methylation analyses or expression analyses (blood, saliva vaginal secretions, menstrual blood, and semen). To discriminate nasal secretion/blood from other secretions (like oral mucosa/saliva, blood, vaginal secretion, menstrual blood, and seminal fluid), assays based on specific methylation patterns at several CpGs were set up in this study. Out of an initial 54 different CpG markers tested, two markers showed a specific methylation value for nasal samples: N21 and N27 with a methylation mean value of 64.4% ± 17.6% and 33.2% ± 8.7%, respectively. Although identification or discrimination was not possible for all nasal samples (due to partial overlap in methylation values to other secretions), 63% and 26% of the nasal samples could be unambiguously identified and distinguished from the other secretions using the CpG marker N21 and N27, respectively. In combination with a blood pretest/rapid test, a third marker (N10) was able to detect nasal cells in 53% of samples. Moreover, the employment of this pretest increases the proportion of identifiable or discriminable nasal secretion samples using marker N27 to 68%. In summary, our CpG assays proved to be promising tools in forensic analysis for the detection of nasal cells in samples from a crime scene., (© 2023. The Author(s).)
- Published
- 2023
- Full Text
- View/download PDF
79. About the influence of environmental factors on the persistence of DNA - a long-term study.
- Author
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Poetsch M, Markwerth P, Konrad H, Bajanowski T, and Helmus J
- Subjects
- Crime, Humans, Plastics, Saliva, DNA, DNA Fingerprinting
- Abstract
DNA persistence and DNA transfer are important features in the assessment of a crime scene. The question how long DNA may persist at a certain location is similarly important as the one how the DNA has been transferred to this location. Depending on the source of the DNA as well as the conditions at the crime scene, the answer to this question is quite difficult. In this study, persistence of DNA from epithelial abrasions, blood cells, and saliva cells in indoor and outdoor scenarios has been investigated with regard to exposure time and exposure conditions including sunlight, temperature, and humidity in summer and winter scenarios. Overall, we generated 338 epithelial samples, 572 blood samples, and 572 saliva samples. A complete profile of the cell/DNA donor after exposure could be obtained in 47%, 65%, and 58% of epithelial abrasions, blood samples, and saliva samples, respectively. Regarding blood samples, there were no differences between supporting materials cloth and plastic; however, the percentage of complete profiles was higher for saliva samples on plastic and for epithelial samples on cloth. In indoor scenarios, complete profiles could be recovered from nearly all blood and saliva samples up to 9 months, whereas the amount of epithelial complete profiles already started to decline after 3 months. In outdoor scenarios, we observed a tipping point at an exposure time of 3 months. Blood and saliva samples collected after this period displayed complete profiles in less than 25% of samples. After 12 months, no outdoor sample showed a complete profile. The results of this study facilitate decisions on the relevance of recovered DNA from crime scenes., (© 2022. The Author(s).)
- Published
- 2022
- Full Text
- View/download PDF
80. Vibration as a pitfall in pyrosequencing analyses.
- Author
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Konrad H, Schäfer L, Sturm H, Hördt L, Bajanowski T, and Poetsch M
- Subjects
- CpG Islands, High-Throughput Nucleotide Sequencing methods, Humans, Reproducibility of Results, Sequence Analysis, DNA methods, DNA Methylation, Vibration
- Abstract
Since methylation analysis has become an important tool in forensic genetics, the reliability and credibility of the method must be ensured. After a successful validation and establishment of several pyrosequencing assays using a PyroMark® Q48 Autoprep instrument (Qiagen, Hilden, Germany), we decided to expand the method further purchasing a second instrument. But after initializing this second instrument side by side with the first, the majority of analyses failed (97 samples of 133 samples (73%)). The number of error messages increased rapidly and the average RFU values decreased. After purchasing two anti-vibration weighing tables for the PyroMark® instruments and repeating the analyses under the same conditions and with identical samples the results improved considerably, 115 samples of 130 samples (88%) showed successful and reproducible results. These findings demonstrate the impact of vibrations and percussions on PyroMark® Q48 Autoprep performance and the reliability of methylation analyses., (© 2021. The Author(s).)
- Published
- 2022
- Full Text
- View/download PDF
81. Inter-laboratory adaption of age estimation models by DNA methylation analysis-problems and solutions.
- Author
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Pfeifer M, Bajanowski T, Helmus J, and Poetsch M
- Subjects
- Amidohydrolases analysis, Blood Chemical Analysis, Cyclic Nucleotide Phosphodiesterases, Type 4 analysis, Edar-Associated Death Domain Protein analysis, Fatty Acid Elongases analysis, Humans, Laboratories, Predictive Value of Tests, Saliva chemistry, Aging genetics, CpG Islands, DNA Methylation, Forensic Genetics methods, Genetic Markers
- Abstract
In recent years, a lot of age prediction models based on different CpG motives in different cell types were published determining the biological age of a person by DNA methylation. For a general employment of this technique, maybe even as a routine method, the cross-laboratory application of such models has to be examined. Therefore, we tested two different published age prediction models for blood and mouth swab samples with regard to prediction accuracy (Bekaert et al Epigenetics 10:922-930, 2015a; Bekaert et al Forensic Sci Int Genet Suppl Ser 5:e144-e145, 2015b). Both models are based on CpG sites of four genes (ASPA, EDARADD, PDE4-C, and ELOVL2), but with a different combination of CpGs for the two tissue types. A mean absolute difference (MAD) between chronological and predicted age of 9.84 and 8.32 years for blood and buccal swab models could be demonstrated, respectively, which is significantly worse than the published data, probably due to higher DNA methylation variances in some CpGs. By retraining both prediction models, the prediction accuracy could be improved to a MAD of 5.55 and 4.65 years for the renewed blood and buccal swab model, respectively. This study demonstrates the usefulness of effective DNA standards to normalize DNA methylation data for better comparison of study results.
- Published
- 2020
- Full Text
- View/download PDF
82. Cleaning a crime scene 2.0-what to do with the bloody knife after the crime?
- Author
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Helmus J, Poetsch J, Pfeifer M, Bajanowski T, and Poetsch M
- Subjects
- Adolescent, Female, Humans, Male, Middle Aged, Blood Stains, DNA isolation & purification, DNA Fingerprinting, Detergents, Epithelial Cells chemistry, Saliva chemistry
- Abstract
The persistence of DNA on washed items as well as the DNA transfer has become a major subject of research in recent years, especially after the detectability of minor DNA traces was heavily increased by sensitive analysis methods. Nowadays, the attribution of a DNA trace to an individual is only rarely questioned, whereas the way of application of this DNA to an item is subject to much discussion and speculation. Additionally, the removal of DNA by cleaning or its possible persistence on an item despite a cleaning process are often important problems in court. The aim of this study was to investigate whether DNA traces (blood, saliva, epithelial cells) on different objects (knives, plates, glasses, and plastic lids) can persist on the surface despite cleaning by different methods like hand-washing or the use of a dishwasher. In total, 120 samples were collected from artificially constructed blood, saliva, and epithelial cell stains on objects with smooth surfaces after washing and analyzed by STR amplification. Samples taken after rinsing or hand-washing resulted mainly in complete DNA profiles (62.5% of samples), while cleaning in the dishwasher rendered almost everything completely DNA-free. Since in the hand-washing experiments a secondary transfer of DNA through the water could not be ruled out, additional transfer experiments were conducted with blood and saliva samples on plates. Here, a carryover of DNA traces could be demonstrated up to the fifth washed item.
- Published
- 2020
- Full Text
- View/download PDF
83. Unintentional effects of cleaning a crime scene-when the sponge becomes an accomplice in DNA transfer.
- Author
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Helmus J, Pfeifer M, Feiner LK, Krause LJ, Bajanowski T, and Poetsch M
- Subjects
- Blood Chemical Analysis, Detergents, Forensic Sciences, Humans, Saliva chemistry, DNA analysis, DNA Fingerprinting
- Abstract
DNA transfer in aqueous solutions as well as the persistence of DNA on washed items has become a major subject of research in recent years and is often a significant problem in court. Despite these approaches, the question about the "mobility" of DNA especially in capital offenses cannot be answered in every case, since a variety of scenarios for DNA transfer are possible. The aim of this study was to investigate whether DNA traces could be distributed by cleaning an object. For this purpose, a large table surface and fabric piece were artificially provided with skin contact traces and body fluids (saliva and blood) in two series of experiments and then wiped off with water or with soap water (218 samples in total). These experiments resulted in a clear "carry over" of DNA traces especially for body fluid samples (100% of blood samples and 75% of saliva samples led to a complete profile). The results could be confirmed in a second experimental set-up with 384 samples using different cleaning agents and more intense cleaning actions. Even small amounts of 5-10 μl body fluid led to complete profiles in around 45% of the samples, while 20 μl led to nearly 65% complete profiles. A strong impact of the amount of traces and the chosen surface could be demonstrated, while the active component of the cleaning agent seemed to be of less influence with the explicit exception of chloric agents which rendered almost everything completely DNA-free. In summary, a distribution of DNA traces by wiping or scrubbing an object could be clearly proven.
- Published
- 2019
- Full Text
- View/download PDF
84. The association between clinical outcome and CD8 + lymphocytic infiltration in advanced stages of colorectal cancer differs by latent virus infection in tumour tissue.
- Author
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Kleist B, Bagdonas M, Oommen P, Schoenhardt I, Levermann J, and Poetsch M
- Subjects
- Adult, Aged, Aged, 80 and over, CD8-Positive T-Lymphocytes immunology, Cytomegalovirus Infections complications, Cytomegalovirus Infections immunology, Female, Herpes Simplex complications, Herpes Simplex immunology, Humans, Male, Middle Aged, Papillomavirus Infections complications, Papillomavirus Infections immunology, Tumor Virus Infections immunology, Virus Latency immunology, Colorectal Neoplasms immunology, Colorectal Neoplasms virology, Lymphocytes, Tumor-Infiltrating immunology, Tumor Virus Infections complications
- Abstract
Aims: In the near future, an immunoscore based on the quantification of lymphocytic populations can be expected as a fundamental supplement of colorectal cancer (CRC) classification. This study explored whether latent viral infection has an influence on prognostically relevant host immunity in CRC., Methods and Results: CD8
+ lymphocytic infiltration in three tumour compartments of 121 CRC was compared with clinical data and occurrence of latent infection with herpes simplex virus (HSV1, HSV2), cytomegalovirus (CMV), human papillomavirus (HPV16 and HPV18) in the tumour tissue, which was determined by polymerase chain reaction (PCR). Intraepithelial CD8+ lymphocytic infiltration (IECD 8+ ) showed a trend towards correlation with clinical stage (P = 0.073), significant differences between CRC with and without metastases (P = 0.001) and a significant correlation with overall survival (OS, P = 0.001). Each of these three clinical parameters showed a significant link to IECD 8+ in the virus DNA-negative (P-values: 0.001-0.036), but no significant differences in the virus DNA-positive subgroup, which is consistent with a moderating effect of virus DNA on these associations. A significant correlation of CD8+ infiltration in the invasive margin (IMCD 8+ ) with OS (P = 0.016) was also moderated by virus DNA., Conclusion: Our data suggest a possible influence of latent viral infection on the association between clinical outcome and CD8+ lymphocytic infiltration in CRC tissue. After confirmation of these results by large cohort studies, a potential interaction between microbial pathogens and host immunity in CRC and its impact on prognostic immunoscores and/or new therapeutic strategies should be investigated further., (© 2017 John Wiley & Sons Ltd.)- Published
- 2018
- Full Text
- View/download PDF
85. Persistence of DNA on clothes after exposure to water for different time periods-a study on bathtub, pond, and river.
- Author
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Helmus J, Zorell S, Bajanowski T, and Poetsch M
- Subjects
- Adult, DNA blood, DNA Fingerprinting, Epithelial Cells chemistry, Female, Forensic Genetics, Humans, Male, Microsatellite Repeats, Middle Aged, Seasons, Time Factors, Baths, Clothing, DNA isolation & purification, Immersion, Ponds, Rivers
- Abstract
DNA traces on clothes of drowned bodies can provide important evidence for police investigations, especially in cases of suspected suicides or homicides. However, it is generally assumed that the water "erodes" a large part of the DNA depending especially on the exposure time. In forensic casework, DNA of suspects could be found frequently on clothes of drowned bodies after hours, sometimes days of exposure to water. This study was conducted to attempt a general statement about the conditions under which sufficient DNA remains can be expected for molecular genetic analysis. For this purpose, different scenarios were designed including DNA from three to five people, different types of waters (tap, pond, bathtub and river) for various time periods, with higher water pressure, different temperature, and soapy water (bathtub). Epithelial cells and blood cells were mounted on cotton cloths, and the DNA left after exposure was analyzed using the Powerplex® ESX17fast kit. In the indoor experiments, complete profiles could be seen even after 10 min rinsing of clothes under the tap and after 1 week in the bathtub. Outdoors, the results differed considerably between summer and winter as well as between pond and river. The longest exposure time still resulting in a complete profile was 2 weeks for a sample with skin cells in the pond during winter. In summer, the time period for erasing the bulk of DNA was 4 hours regarding epithelial samples and more than 1 day for blood samples in pond and river environments. All in all, the results demonstrate that DNA could still be recovered from clothes exposed to water for more than 1 week.
- Published
- 2018
- Full Text
- View/download PDF
86. Impact of several wearers on the persistence of DNA on clothes-a study with experimental scenarios.
- Author
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Poetsch M, Pfeifer M, Konrad H, Bajanowski T, and Helmus J
- Subjects
- Electrophoresis, Humans, Microsatellite Repeats, Multiplex Polymerase Chain Reaction, Clothing, DNA analysis, DNA Fingerprinting
- Abstract
The detection of DNA of a certain person on the inside of a piece of clothing involved in a crime scene is usually seen as confirmation that this person is the owner or bearer and therefore participated in this crime. However, besides the possibilities of secondary or even tertiary transfer of DNA, the accused often argues that he lent the garment to another person who by chance did not leave any DNA while committing the crime. Then, forensic genetic scientists have to answer the question how long DNA persists on an item used in daily routine and how long a piece of clothing must be worn to definitively leave detectable DNA behind. In an attempt to answer these questions, several scenarios with two or three individuals wearing the same sweatband for different time periods were set up. DNA left on the sweatbands was isolated, quantified, and then analyzed using the Powerplex® ESX17fast kit. The majority of samples displayed all alleles of both/all three wearers on the outside (67%) as well as on the inside (80%) of the sweatbands. In contrast, a single profile of the first wearer could only be found once among all 204 samples, a single profile of the second wearer in 7% of samples. Wearing the sweatband for only 10 min was enough to result in a complete profile of the second wearer in 79% of samples. So, it is highly unlikely to wear/use a piece of clothing for even a short period of time without leaving own DNA behind.
- Published
- 2018
- Full Text
- View/download PDF
87. Mitochondrial DNA alteration in primary and metastatic colorectal cancer: Different frequency and association with selected clinicopathological and molecular markers.
- Author
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Kleist B, Meurer T, and Poetsch M
- Subjects
- Adult, Aged, Aged, 80 and over, Class I Phosphatidylinositol 3-Kinases, Female, GTP Phosphohydrolases genetics, Humans, Lymphatic Metastasis genetics, Male, Membrane Proteins genetics, Middle Aged, Mutation Rate, Phosphatidylinositol 3-Kinases genetics, Proto-Oncogene Proteins B-raf genetics, Proto-Oncogene Proteins p21(ras) genetics, Biomarkers, Tumor genetics, Colorectal Neoplasms genetics, Colorectal Neoplasms pathology, DNA, Mitochondrial genetics, Microsatellite Instability
- Abstract
This study attempts to determine whether primary tumor tissue could reliably represent metastatic colorectal cancer in therapy-guiding analysis of mitochondrial microsatellite instability. Therefore, we investigated the concordance of microsatellite instability in D310, D514, and D16184 (mitochondrial DNA displacement loop), and its association with selected clinical categories and KRAS/NRAS/BRAF/PIK3CA/TP53 mutation status between primary and metastatic colorectal cancer tissue from 119 patients. Displacement loop microsatellite instability was significantly more frequently seen in lymph node metastases (53.1%) compared to primary tumors (37.5%) and distant metastases (21.4%) ( p = 0.0183 and p = 0.0005). The discordant rate was significantly higher in lymph node metastases/primary tumor pairs (74.6%) than in distant metastases/primary tumor pairs (52.4%) or lymph node metastases/distant metastases pairs (51.6%) ( p = 0.0113 and p = 0.0261) with more gain (86.7%) than loss (61.1%) of microsatellite instability in the discordant lymph node metastases ( p = 0.0024). Displacement loop instability occurred significantly more frequently in lymph node metastases and distant metastases of patients with early colorectal cancer onset age <60 years ( p = 0.0122 and p = 0.0129), was found with a significant high rate in a small cohort of TP53-mutated distant metastases ( p = 0.0418), and was associated with TP53 wild-type status of primary tumors ( p = 0.0009), but did not correlate with KRAS, NRAS, BRAF, or PIK3CA mutations. In conclusion, mitochondrial microsatellite instability and its association with selected clinical and molecular markers are discordant in primary and metastatic colorectal cancer, which could have importance for surveillance and therapeutic strategies.
- Published
- 2017
- Full Text
- View/download PDF
88. The role of known variants of KCNQ1, KCNH2, KCNE1, SCN5A, and NOS1AP in water-related deaths.
- Author
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Tzimas I, Zingraf JC, Bajanowski T, and Poetsch M
- Subjects
- Adaptor Proteins, Signal Transducing genetics, Adult, Case-Control Studies, Channelopathies genetics, ERG1 Potassium Channel genetics, Gene Frequency, Genotype, Humans, KCNQ1 Potassium Channel genetics, Middle Aged, NAV1.5 Voltage-Gated Sodium Channel genetics, Potassium Channels, Voltage-Gated genetics, Young Adult, Death, Sudden, Cardiac etiology, Drowning diagnosis, Polymorphism, Single Nucleotide
- Abstract
Drowning is one of the most frequent causes of accidental deaths worldwide, and still it remains a diagnosis of exclusion. Moreover, sudden cardiac deaths (SCD) or, if no actual cardiac alterations can be found, sudden unexplained deaths (SUD) represent a major group within mortality statistics as well. This leads to the assumption that there might be a general underlying cause for at least some cases of drowning, SCD, or SUD, for example, genetic aberrations in arrhythmia-associated genes. In the present study, blood samples of 171 corpses found in water (drowning, death after almost drowning, and unclear deaths) were analyzed in 19 known variants of the genes KCNQ1, KCNH2, KCNE1, SCN5A, and NOS1AP by minisequencing. In three variants of NOS1AP, significant differences of allele and/or genotype frequencies could be demonstrated between victims of drowning and published controls as well as own controls. Moreover, similar differences were found comparing unexplained deaths in water and controls. Regarding the other genes, especially one single nucleotide polymorphism (SNP) of KCNQ1 could be associated with drowning. These results propose that performing a molecular autopsy analyzing known variants of arrhythmia-associated genes, in particular NOS1AP, may assist in establishing a cause of death for bodies found in water without clear drowning signs.
- Published
- 2016
- Full Text
- View/download PDF
89. Effects of ionic strength and fulvic acid on adsorption of Tb(III) and Eu(III) onto clay.
- Author
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Poetsch M and Lippold H
- Subjects
- Adsorption, Calcium Chloride chemistry, Clay, Humic Substances, Magnesium Chloride chemistry, Models, Theoretical, Osmolar Concentration, Radioactive Waste, Sodium Chloride chemistry, Aluminum Silicates chemistry, Benzopyrans chemistry, Europium chemistry, Terbium chemistry
- Abstract
High salinity and natural organic matter are both known to facilitate migration of toxic or radioactive metals in geochemical systems, but little is known on their combined effect. We investigated complexation of Tb(III) and Eu(III) (as analogues for trivalent actinides) with fulvic acid and their adsorption onto a natural clay in the presence of NaCl, MgCl2 and CaCl2 up to very high ionic strengths. (160)Tb, (152)Eu and (14)C-labelled fulvic acid were employed as radiotracers, allowing investigations at very low concentrations according to probable conditions in far-field scenarios of nuclear waste repositories. A combined Kd approach (Linear Additive Model) was tested for suitability in predicting solid-liquid distribution of metals in the presence of organic matter based on the interactions in the constituent subsystems. In this analysis, it could be shown that high ionic strength does not further enhance the mobilizing potential of humic matter. A quantitative reproduction of the influence of fulvic acid failed for most systems under study. Assumptions and limitations of the model are discussed., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
90. Does zero really mean nothing?-first experiences with the new PowerQuant(TM) system in comparison to established real-time quantification kits.
- Author
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Poetsch M, Konrad H, Helmus J, Bajanowski T, and von Wurmb-Schwark N
- Subjects
- Humans, Microsatellite Repeats, Reproducibility of Results, DNA analysis, DNA Fingerprinting, Real-Time Polymerase Chain Reaction instrumentation
- Abstract
DNA quantification is an important step in the molecular genetic analysis of a forensic sample, hopefully providing reliable data on DNA content for a subsequent generation of reproducible STR profiles for identification. For several years, this quantification has usually been done by real-time PCR protocols and meanwhile a variety of assays are commercially available from different companies. The newest one is the PowerQuant(TM) assay by Promega Inc. which is advertised with the promise that a determined DNA concentration of 0 ng/μl in a forensic sample guarantees the impossibility to achieve true STR results, thus allowing to exclude such samples from STR analysis to save time and money. Thus, the goal of this study was to thoroughly verify the quantification step with regard to its suitability as a screening method. We have evaluated the precision and reliability of four different real-time PCR quantification assays by systematically testing DNA dilutions and forensic samples with various DNA contents. Subsequently, each sample was subjected to the Powerplex® ESX 17 fast kit to determine a reliable cutoff level for exclusion of definitely negative samples from STR analysis. An accurate quantification of different cell line DNA dilutions was not possible with any kit. However, at least the PowerQuant(TM) assay provided suitable data analyzing forensic samples, whereas in other systems up to 46 % of negative samples still displayed reliable STR analysis results. All in all, the PowerQuant(TM) assay represents a big step forward, but the evaluation of real-time PCR quantification results has still to be done with great care.
- Published
- 2016
- Full Text
- View/download PDF
91. Correlation between DPYD gene variation and KRAS wild type status in colorectal cancer.
- Author
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Kleist B, Kempa M, Meurer T, and Poetsch M
- Subjects
- Adult, Aged, Aged, 80 and over, Antimetabolites, Antineoplastic adverse effects, Antimetabolites, Antineoplastic metabolism, Colorectal Neoplasms drug therapy, Colorectal Neoplasms metabolism, Colorectal Neoplasms pathology, DNA Mutational Analysis, ErbB Receptors antagonists & inhibitors, ErbB Receptors metabolism, Female, Fluorouracil adverse effects, Fluorouracil metabolism, Gene Expression Profiling methods, Genetic Predisposition to Disease, Humans, Male, Middle Aged, Multiplex Polymerase Chain Reaction, Pharmacogenetics, Phenotype, Precision Medicine, Predictive Value of Tests, Protein Kinase Inhibitors therapeutic use, Colorectal Neoplasms genetics, Dihydrouracil Dehydrogenase (NADP) genetics, Mutation, Polymorphism, Genetic, Proto-Oncogene Proteins p21(ras) genetics
- Abstract
Aims: Failure and side effects of combined cytotoxic therapy are challenges in the treatment of metastatic colorectal cancer (CRC). DPYD gene variations can potentially predict toxicity to 5-fluorouracil (FU)-based therapy and KRAS-, NRAS-, BRAF-, PIK3CA-wild type status is a known prerequisite for epidermal growth factor receptor (EGFR) inhibitor therapy. This study was performed to search for a possible link between these therapeutic markers., Methods: The DPYD gene variations c.496A>G, c.1679T>G, c.2846A>T and KRAS/NRAS/BRAF/PIK3CA mutational status were determined in non-neoplastic, primary CRC and metastatic CRC tissue from 115 patients., Results: The polymorphism c.496A>G was the DPYD gene variant with the highest detection rate (12.9%), occurred predominantly in females (86.7%, p=0.0044) and was exclusively seen in KRAS wild type primary CRC (15/65 (23.1%) vs 0/51 (0%) in KRAS-mutated primary CRC, respectively, p=0.0001)., Conclusions: This genetic profile could define a patient group requiring alternative combined therapeutic approaches. Global testing of large patient cohorts is necessary to prove this concept., (Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/)
- Published
- 2016
- Full Text
- View/download PDF
92. The role of hereditary KCNQ1 mutations in water-related death.
- Author
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Tzimas I, Bajanowski T, and Poetsch M
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Arrhythmias, Cardiac genetics, Child, Child, Preschool, Exons, Female, Humans, Male, Middle Aged, Young Adult, Drowning mortality, KCNQ1 Potassium Channel genetics, Mutation
- Abstract
Drowning remains one of the major causes of death in most developed countries despite the fact that many of the victims are known to be at least moderate swimmers as well as healthy directly before the event. Here, fatal arrhythmias and especially the long QT syndrome (LQTS) have been proposed as the underlying mechanism which may be connected to mutations in one of the associated genes. The KCNQ1 gene is involved in the occurrence of LQT1 which may be triggered by swimming. Therefore, 176 cases of drowning were screened for mutations in the exons 3, 5, 6, 7, and 8 of the KCNQ1 gene which have been shown to harbor major mutation clusters. No variation to the published sequence could be found in the exonic DNA in any of the cases clearly disproving an involvement of these mutation clusters in cases of drowning.
- Published
- 2016
- Full Text
- View/download PDF
93. DNA transfer-a never ending story. A study on scenarios involving a second person as carrier.
- Author
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Helmus J, Bajanowski T, and Poetsch M
- Subjects
- Adult, Alleles, Epithelial Cells chemistry, Female, Humans, Male, Microsatellite Repeats, Middle Aged, Multiplex Polymerase Chain Reaction, Real-Time Polymerase Chain Reaction, DNA analysis, DNA Fingerprinting, Touch
- Abstract
The transfer of DNA directly from one item to another has been shown in many studies with elaborate discussions on the nature of the DNA donor as well as material and surface of the items or surrounding features. Every DNA transfer scenario one can imagine seems to be possible. This evokes more and more intricate scenarios proposed by lawyers or attorneys searching for an explanation of the DNA of a certain person on a distinct item with impact on a crime. At court, the forensic genetic scientist has to comment on the probability of these scenarios thus calling for extensive studies on such settings. Here, the possibility of an involvement of a second person as a carrier of the donor's DNA in a variety of different scenarios including three pairs of people and two kinds of items (textiles and plastic bags) was investigated. All transfer settings were executed with and without gloves on the carrier's hands. DNA left on the items was isolated and analyzed using the Powerplex® ESX17 kit. In 21 out of 180 samples, all alleles of the donor DNA could be obtained on the second item (12%), on eight samples, the donor's DNA was dominant compared to all other alleles (38% of samples with complete donor profile). Additionally, 51 samples displayed at least more than half of the donor's alleles (28%). The complete DNA profile of the carrier was found in 47 out of 180 samples (42 partial profiles). In summary, it could be shown that a transfer of donor DNA from epithelial cells through a carrier to a second item is possible, even if the carrier does not wear gloves.
- Published
- 2016
- Full Text
- View/download PDF
94. Neuroendocrine differentiation: The mysterious fellow of colorectal cancer.
- Author
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Kleist B and Poetsch M
- Subjects
- Animals, Biomarkers metabolism, Cell Lineage, Colorectal Neoplasms epidemiology, Colorectal Neoplasms metabolism, Colorectal Neoplasms therapy, Enteroendocrine Cells metabolism, Humans, Intestinal Mucosa metabolism, Neoplastic Stem Cells metabolism, Neuroendocrine Tumors epidemiology, Neuroendocrine Tumors metabolism, Neuroendocrine Tumors therapy, Phenotype, Prognosis, Signal Transduction, Cell Differentiation, Colorectal Neoplasms pathology, Enteroendocrine Cells pathology, Intestinal Mucosa pathology, Neoplastic Stem Cells pathology, Neuroendocrine Tumors pathology
- Abstract
Neuroendocrine differentiation in sporadic colorectal cancer has been recognized since decades, but its clinical impact is still controversially discussed. Detailed parameter analyses hint at the possibility that probably not neuroendocrine differentiation itself, but its association with poor grade of tumor differentiation, lymph node metastases, distant metastases and other unfavorable features contribute to worse clinical outcome. However, other studies deny a relationship between neuroendocrine differentiation and prognosis of colorectal cancer. This review elucidates, whether new insights into the origin of neuroendocrine differentiation in the intestinal epithelium, its regulation by mTOR pathway components and its possible link to the intestinal stem cell compartment could determine a role of neuroendocrine cells as prognostic marker and putative therapeutic target in sporadic colorectal cancer.
- Published
- 2015
- Full Text
- View/download PDF
95. About the power of biostatistics in sibling analysis-comparison of empirical and simulated data.
- Author
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von Wurmb-Schwark N, Podruks E, Schwark T, Göpel W, Fimmers R, and Poetsch M
- Subjects
- Genetic Markers, Humans, Multiplex Polymerase Chain Reaction, DNA Fingerprinting, Likelihood Functions, Microsatellite Repeats, Siblings, Twins, Dizygotic genetics
- Abstract
The determination of potential sibship is a common task in routine kinship analysis, but often the putative parents are not available for analysis anymore. Then, a sibling analysis has to be conducted investigating only the potential siblings, thus reducing the power of the conclusion. In an attempt to determine meaningfulness of biostatistical calculations, 346 dizygotic twin pairs, 30 confirmed half siblings, and 112 unrelated people (to generate 6216 pair comparisons) were studied, all genetically typed using at least the Powerplex® 16 STRs. From every pair, the probabilities for a full sibship (identical parents) and half sibship (different fathers) were calculated using a commercially available computer program. Additionally, we simulated marker data for one million pairs of full sibs, half sibs, and unrelated persons each. Ninety-five percent of full sibling pairs demonstrated a likelihood ratio (LR) > 9 (W-value > 90 %) and less than 4% of these showed a LR < 3 (W-value < 75%) for full sibship after analysis of 15 STRs. The results for half siblings are less unambiguous. Here, only 57% achieved a LR > 9 and 23% a LR < 3. Regarding the unrelated pairs, more than 90% had a LR < 1/9 and only 2% reached a LR > 9. All in all, our results show that 15 to 20 STRs have sufficient power for analyses in kinship. Moreover, our data provide a statistical basis for the determination of the information content of a LR/W-value in a sibship case. Investigating an identical number of full siblings and unrelated pairs, it could be shown that 92% of pairs with a LR > 9 for full sibship probability really are full siblings. So, setting a cutoff level for full sibship at LR > 9, less than 10% of pairs will be wrongly assumed as full siblings even though they are unrelated.
- Published
- 2015
- Full Text
- View/download PDF
96. That's not it, either-neither polymorphisms in PHOX2B nor in MIF are involved in sudden infant death syndrome (SIDS).
- Author
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Poetsch M, Todt R, Vennemann M, and Bajanowski T
- Subjects
- Case-Control Studies, Female, Gene Frequency, Humans, Infant, Infant, Newborn, Male, Promoter Regions, Genetic, Homeodomain Proteins genetics, Intramolecular Oxidoreductases genetics, Macrophage Migration-Inhibitory Factors genetics, Polymorphism, Single Nucleotide, Sudden Infant Death genetics, Transcription Factors genetics
- Abstract
The occurrence of sudden infant death syndrome (SIDS) has been linked to several genetic risk factors, e.g. genes involved in the neuroadrenergic system, variations in serotonin reporter genes or mutations in long-QT syndrome genes. Additionally, polymorphisms in genes with impact in sleep disorder syndromes have been proposed to be of importance as genetic risk factors for SIDS. In this study, we investigated the polyalanine length variation of PHOX2B and the -794 CATT repeat in the MIF promoter region as well as single nucleotide polymorphisms (rs28462174, rs28727473, rs16853571, rs755622, rs12485058, rs12485068, rs4822444, rs4822445, rs4822446, rs4822447 and rs2012124) in both genes in 278 SIDS cases and 240 controls. No significant differences were found in allele distribution of neither length polymorphisms nor single nucleotide polymorphisms between SIDS cases or controls. Therefore, an importance of these variations for the occurrence of SIDS could be ruled out.
- Published
- 2015
- Full Text
- View/download PDF
97. Establishment of a Cell Line From Conjunctival Squamous Cell Carcinoma: PeCa-UkHb-01.
- Author
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Thomasen H, Müller B, Poetsch M, Steuhl KP, and Meller D
- Subjects
- Aged, Biomarkers, Tumor metabolism, Carcinoma, Squamous Cell genetics, Carcinoma, Squamous Cell metabolism, Cell Line, Tumor, Cell Proliferation, Cell Separation, Conjunctival Neoplasms genetics, Conjunctival Neoplasms metabolism, Fluorescent Antibody Technique, Indirect, Humans, Karyotyping, Male, Microsatellite Repeats genetics, Primary Cell Culture, Real-Time Polymerase Chain Reaction, Tissue Donors, Tumor Cells, Cultured, Carcinoma, Squamous Cell pathology, Conjunctival Neoplasms pathology
- Abstract
Purpose: Until now, no epithelial cell line from conjunctival squamous cell carcinoma (SCC), to our knowledge, has existed; therefore, the establishment of a model cell line would be a useful tool for further studies. In particular, the phenotypic and molecular characterization in comparison to other SCC cells is of high interest because this would enable the development of new treatment options for clinical application in ophthalmic oncology., Methods: Epithelial cells were isolated from a bulbar conjunctiva SCC obtained from a 74-year-old male, harvested by stepwise trypsinization and named PeCa-UkHb-01. Cell doubling and the number of passages were determined. Short tandem repeats (STR) and karyotype analyses were performed. Semiquantitative real-time PCR and immunocytochemical fluorescence staining were carried out to detect tumor and epithelial cell markers., Results: The cells had an epithelial and conjunctival phenotype. They grew above passage number 50 in a doubling time at approximately 34.5 hours. Short tandem repeat analyses confirmed the cell origin, although loss of heterozygosity occurred. Karyotype analyses revealed a heterogeneous composition of the cell culture and the karyogram itself showed aberrations and changes in the chromosome numbers. Real-time PCR and immunocytochemical fluorescence staining revealed the expression of the stem cell markers such as ABCG2, p63, OCT4, c-MYC, and SOX2 as well as the conjunctival cytokeratin K19., Conclusions: PeCa-UkHb-01 cells fulfill the criteria of a cell line. They display characteristics of ocular carcinoma cells and therefore the presented cell line might serve for further basic research in ophthalmic oncology.
- Published
- 2015
- Full Text
- View/download PDF
98. Everything clean? Transfer of DNA traces between textiles in the washtub.
- Author
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Kamphausen T, Fandel SB, Gutmann JS, Bajanowski T, and Poetsch M
- Subjects
- Adult, Blood Stains, DNA Fingerprinting, Electrophoresis, Epithelial Cells cytology, Female, Humans, Male, Middle Aged, Mouth Mucosa cytology, Multiplex Polymerase Chain Reaction, DNA isolation & purification, Hand Disinfection, Laundering, Textiles
- Abstract
Forensic genetic analysis of items possibly handled by a suspect or a victim is frequently inquired by the law enforcement authorities, since DNA left on touched objects can often be linked to an individual. Due to technical improvement, even poor traces, which seemed to be unsuitable for DNA analysis a few years ago, may be amplified successfully today. Yet, DNA can be transferred to a crime scene artificially or unintentionally without any primary contact between the individual and the object found at the crime scene, the so-called secondary transfer or indirect transfer in general. In this study, "secondary transfer" scenarios with cells and DNA of different origins under wet conditions were investigated. Transfer was simulated as either "washing by hand" in a washtub or as "machine laundry" in a washing machine. As expected, major differences were seen between blood stains and epithelial abrasions. DNA from blood donors could be detected clearly both on the donor and on the acceptor textile, regardless of washing method. Regarding epithelial abrasions, simulating worn clothes, after washing by hand, only little residual DNA was found, and partial profiles were displayed on the donor textile, while transfer to the acceptor textile occurred even less and not in noteworthy amount and quality. Single alleles could be found both on donor textiles and acceptor textiles after simulated machine wash, but no reliable DNA profile could be verified after laundry in machine. Therefore, a DNA transfer from one worn cloth (without blood stains) to another textile in the washing machine seems to be extremely unlikely.
- Published
- 2015
- Full Text
- View/download PDF
99. The auditory ossicles as a DNA source for genetic identification of highly putrefied cadavers.
- Author
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Schwark T, Modrow JH, Steinmeier E, Poetsch M, Hasse J, Fischer H, and von Wurmb-Schwark N
- Subjects
- Adult, Aged, Aged, 80 and over, Female, Forensic Genetics, Humans, Male, Microsatellite Repeats genetics, Middle Aged, Polymerase Chain Reaction, Reproducibility of Results, Young Adult, DNA Fingerprinting methods, Ear Ossicles metabolism, Pedigree, Postmortem Changes
- Abstract
Genetic identification of putrefied bodies is a common task in forensic medicine. With advancing putrefaction, however, DNA integrity is rapidly decreasing and genetic typing of tissue might be impaired or impossible. Since DNA stability is generally higher in hard tissues, bones or teeth are frequently used as DNA source in such cases. However, isolation of DNA from hard tissues is usually very time-consuming and labor-intensive. This can be especially important in (forensic) cases where time is short and identification has to be carried out as fast as possible. Here, we present the identification of dead bodies by analyzing DNA from the auditory ossicles. These minuscule bones provided DNA of sufficient quality and quantity for identification purposes in all 40 investigated cases. Additionally, processing of the bones proved to be amazingly easy and fast, and a successful extraction is possible using a variety of different methods. We present a detailed protocol, results, and cases in which this new method has been successfully applied.
- Published
- 2015
- Full Text
- View/download PDF
100. Comparison of neuroendocrine differentiation and KRAS/NRAS/BRAF/PIK3CA/TP53 mutation status in primary and metastatic colorectal cancer.
- Author
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Kleist B, Kempa M, Novy M, Oberkanins C, Xu L, Li G, Loland C, and Poetsch M
- Subjects
- Adult, Aged, Aged, 80 and over, Biopsy, Class I Phosphatidylinositol 3-Kinases, Colorectal Neoplasms enzymology, DNA Mutational Analysis, Female, Humans, Immunohistochemistry, Lymphatic Metastasis, Male, Middle Aged, Neuroendocrine Tumors enzymology, Polymorphism, Single Nucleotide, Predictive Value of Tests, Prognosis, Proto-Oncogene Proteins p21(ras), Biomarkers, Tumor genetics, Cell Differentiation, Colorectal Neoplasms genetics, Colorectal Neoplasms pathology, GTP Phosphohydrolases genetics, Membrane Proteins genetics, Mutation, Neuroendocrine Tumors genetics, Neuroendocrine Tumors secondary, Phosphatidylinositol 3-Kinases genetics, Proto-Oncogene Proteins genetics, Proto-Oncogene Proteins B-raf genetics, Tumor Suppressor Protein p53 genetics, ras Proteins genetics
- Abstract
Neuroendocrine differentiation of tumor tissue has been recognized as an important prerequisite for new targeted therapies. To evaluate the suitability of colorectal cancer (CRC) tissue for these treatment approaches and to find a possible link to pretherapeutic conditions of other targeted strategies, we compared neuroendocrine differentiation and KRAS/NRAS/BRAF/PIK3CA/TP53 mutational status in primary and metastatic CRC. Immunohistochemical expression analysis of neuroendocrine markers chromogranin A and synaptophysin was performed on archival CRC tissue, comprising 116 primary tumors, 258 lymph node metastases and 72 distant metastases from 115 patients. All CRC samples but 30 distant metastases were subjected to mutation analysis of KRAS, NRAS, BRAF, PIK3CA, and TP53. Neuroendocrine marker expression was found significantly less frequently in lymph node metastases compared to primary tumors and distant metastases (20%, 31%, 28%, respectively, P = 0.044). KRAS mutation rates increased significantly from primary tumors to lymph node metastases and distant metastases within the neuroendocrine negative CRC group (44%, 53%, 64%, respectively, P = 0.042). Neuroendocrine differentiation was significantly less concordant than KRAS/NRAS/BRAF/PIK3CA/TP53 mutational status in primary tumor/lymph node metastases pairs (65% versus 88%-99%; P < 0.0001) and primary tumor/distant metastases pairs (64% versus 83%-100%; P = 0.027 and P < 0.0001, respectively). According to these data, therapeutic targeting of neuroendocrine tumor cells can be considered only for a subset of CRC patients and biopsies from the metastatic site should be used to guide therapy. A possible importance of lacking neuroendocrine differentiation for progression of KRAS mutant CRC should be further investigated.
- Published
- 2014
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