262 results on '"Peluso I"'
Search Results
52. Interleukin-21 regulates the T helper cell type 1 transcription factor T-bet in celiac disease mucosa
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Gioia, V., Peluso, I., Caruso, R., Fina, D., Stolfi, C., Tosti, C., Andrei, F., Del Vecchio Blanco, G., Naccari, G.C., Bellinvia, S., Pallone, F., and Monteleone, G.
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- 2006
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53. A functional role of interleukin-21 (IL-21) in promoting matrix metalloproteinases synthesis by gastric epithelial cells during helicobacter pylori infection
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Caruso, R., Tosti, C., Fina, D., Peluso, I., Gioia, V., Stolfi, C., Andrei, F., MacDonald, T., Pallone, F., and Monteleone, G.
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- 2006
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54. Regulation of matrix metalloproteinases (MMPs) synthesis by interleukin-21 (IL-21) in human intestinal fibroblasts
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Peluso, I., Caruso, R., Fina, D., Gioia, V., Stolfi, C., MacDonald, T., Pallone, F., and Monteleone, G.
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- 2006
- Full Text
- View/download PDF
55. Blockade of interleukin (IL)-21 enhances CD3+T cell death in the gut of patients with inflammatory bowel disease (IBD)
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Fina, D., Peluso, I., Caruso, R., Gioia, V., Stolfi, C., Tosti, C., Andrei, F., Naccari, G.C., Bellinvia, S., Pallone, F., and Monteleone, G.
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- 2006
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56. Cyclooxygenase-2 (Cox-2) independent inhibition of proliferation of colon cancer cells by 5-aminosalicylic acid (5-ASA)
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Stolfi, C., Fina, D., Caruso, R., Peluso, I., Gioia, V., Naccari, G.C., Bellinvia, S., Andrei, F., Tosti, C., Pallone, F., and Monteleone, G.
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- 2006
- Full Text
- View/download PDF
57. Regulation of treg cells by lactobacillus paracasei B21060
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Peluso, I., Fina, D., Caruso, R., Gioia, V., Stolti, C., Caspani, G., Grossi, E., Pallone, E., and Monteleone, G.
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- 2006
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- View/download PDF
58. An extensive search for RFLP in the human glucose-6-phosphate dehydrogenase locus has revealed a silent mutation in the coding sequence
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D Urso, M., Luzzatto, L., Perroni, L., Alfredo Ciccodicola, Gentile, G., Peluso, I., Persico, M. G., Pizzella, T., Toniolo, D., and Vulliamy, T. J.
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Male ,Polymorphism, Genetic ,X Chromosome ,Genes ,Mutation ,Humans ,Female ,DNA Restriction Enzymes ,Glucosephosphate Dehydrogenase ,Polymorphism, Restriction Fragment Length ,Research Article ,Pedigree - Abstract
The genetic polymorphism of an approximately 100-kb DNA region comprising and flanking the glucose-6-phosphate dehydrogenase (G6PD) gene on human chromosome Xq28 has been analyzed in detail. By using 14 unique sequence probes and 18 restriction enzymes, we have characterized 257 restriction fragments or 370 restriction sites. On testing 12-57 individual X chromosomes, all sites but one were nonpolymorphic. However, a PstI site that maps to exon 10 of the G6PD gene, which is still monomorphic in all British and Italian subjects tested, is polymorphic in west-African people. Specifically, it is absent from 22% of Nigerian X chromosomes. By sequence analysis we have shown that the absence of this PstI site results from a G----A replacement at position 1116, corresponding to the third base of a glutamine codon; no amino acid change is produced in the protein. Thus, a polymorphic silent mutation is demonstrated in a human gene.
59. The Ceratitis capitata homologue of the Drosophila sex-determining gene Sex-lethal is structurally conserved, but not sex-specifically regulated
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giuseppe saccone, Peluso, I., Artiaco, D., Giordano, E., Bopp, D., Polito, L. C., University of Zurich, and Saccone, G
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1309 Developmental Biology ,1312 Molecular Biology ,570 Life sciences ,biology ,10124 Institute of Molecular Life Sciences
60. High fat meal increase of il-17 is prevented by ingestion of fruit juice drink in healthy overweight subjects
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Serafini, M., Peluso, I., Raguzzini, A., Roberts, S., Villano, D., Cesqui, E., Catasca, G., Toti, E., and Miglio, C.
61. Health benefits of tea
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Mauro Serafini, Del Rio, D., N Dri, Y. D., Bettuzzi, S., and Peluso, I.
62. ACQUIRED HEMOPHILIA IN ELDERLY PATIENTS WITH NON HODGKIN'S LYMPHOMA
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Claudio Cerchione, Luponio, S., Della Pepa, R., Migliaccio, I., Beneduce, G., Pugliese, N., Seneca, E., Cimino, E., Coppola, A., Cerbone, A. M., Tufano, A., Notarangelo, M., Peluso, I., Di Minno, G., Pane, F., and Renzo, A.
63. CARFILZOMIB-LENALIDOMIDE-DEXAMETHASONE IN THE MANAGEMENT OF LENALIDOMIDE-REFRACTORY MULTIPLE MYELOMA: A REAL-LIFE EXPERIENCE
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Claudio Cerchione, Ferrara, K., Peluso, I., Di Perna, M., Zacheo, I., Nappi, D., Pareto, A. E., Pane, F., and Catalano, L.
64. BENDAMUSTINE-BORTEZOMIB-DEXAMETHASONE (BVD) IN THE MANAGEMENT OF RELAPSED AND REFRACTORY MULTIPLE MYELOMA: A REAL-LIFE EXPERIENCE
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Claudio Cerchione, Catalano, L., Pareto, A. E., Nappi, D., Di Perna, M., Basile, S., Marano, L., Peluso, I., Simeone, L., Vitagliano, O., Palmieri, S., Rocco, S., Ferrara, F., and Pane, F.
65. POMALIDOMIDE-DEXAMETHASONE IN THE MANAGEMENT OF HEAVILY PRETREATED MULTIPLE MYELOMA
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Claudio Cerchione, Nappi, D., Pareto, A. E., Migliaccio, I., Zacheo, I., Di Perna, M., Peluso, I., Ferrara, K., Pane, F., and Catalano, L.
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Cancer Research ,Oncology ,Hematology
66. Insulin Resistance, Acanthosis Nigricans, and Polycystic Ovaries Associated with a Circulating Inhibitor of Postbinding Insulin Action
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HARRISON, L.C., primary, DEAN, B., additional, PELUSO, I., additional, CLARK, S., additional, and WARD, G., additional
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- 1985
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67. miRNeye: a microRNA expression atlas of the mouse eye
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Verde Roberta, Bilio Marchesa, Gennarino Vincenzo A, Peluso Ivana, Karali Marianthi, Lago Giampiero, Dollé Pascal, and Banfi Sandro
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Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background MicroRNAs (miRNAs) are key regulators of biological processes. To define miRNA function in the eye, it is essential to determine a high-resolution profile of their spatial and temporal distribution. Results In this report, we present the first comprehensive survey of miRNA expression in ocular tissues, using both microarray and RNA in situ hybridization (ISH) procedures. We initially determined the expression profiles of miRNAs in the retina, lens, cornea and retinal pigment epithelium of the adult mouse eye by microarray. Each tissue exhibited notably distinct miRNA enrichment patterns and cluster analysis identified groups of miRNAs that showed predominant expression in specific ocular tissues or combinations of them. Next, we performed RNA ISH for over 220 miRNAs, including those showing the highest expression levels by microarray, and generated a high-resolution expression atlas of miRNAs in the developing and adult wild-type mouse eye, which is accessible in the form of a publicly available web database. We found that 122 miRNAs displayed restricted expression domains in the eye at different developmental stages, with the majority of them expressed in one or more cell layers of the neural retina. Conclusions This analysis revealed miRNAs with differential expression in ocular tissues and provided a detailed atlas of their tissue-specific distribution during development of the murine eye. The combination of the two approaches offers a valuable resource to decipher the contributions of specific miRNAs and miRNA clusters to the development of distinct ocular structures.
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- 2010
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68. The clot gene of Drosophila melanogaster encodes a conserved member of the thioredoxin-like protein superfamily
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A. Digilio, M. Furia, R. Rendina, E. Giordano, I. Peluso, Giordano, Ennio, Peluso, I, Rendina, R, Digilio, A., Furia, Maria, Peluso, I., and Rendina, R.
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Mutant ,Molecular Sequence Data ,Genes, Insect ,Protein Structure, Secondary ,Mice ,Thioredoxins ,Glutaredoxin ,Genetics ,Animals ,Drosophila Proteins ,Humans ,Amino Acid Sequence ,Allele ,Sepia ,Cloning, Molecular ,Molecular Biology ,Gene ,Conserved Sequence ,Glutaredoxins ,Cloning ,biology ,Base Sequence ,Sequence Homology, Amino Acid ,Pteridines ,Genetic Complementation Test ,Gene Expression Regulation, Developmental ,Proteins ,General Medicine ,DNA ,Protein superfamily ,biology.organism_classification ,Glutathione ,Cell biology ,Drosophila melanogaster ,Oxidoreductases ,Oxidation-Reduction - Abstract
The conversion of pyruvoyl-H4-pterin to pyrimidodiazepine (PDA), which is an essential step in the biosynthesis of the red components of Drosophila eye pigments known as drosopterins, requires the products of the genes sepia and clot. While the product of sepia has been shown to correspond to the enzyme PDA-synthase, the role of clot remains unknown, although the clot 1 allele was one of the first eye-color mutants to be isolated in Drosophila melanogaster, and much genetic and biochemical data has become available since. Here we report the cloning of the clot gene, describe its molecular organization and characterize the sequence alterations associated with the alleles cl 1 and cl 2 . The coding properties of the gene show that it encodes a protein related to the Glutaredoxin class of the Thioredoxin-like enzyme superfamily, conserved members of which are found in human, mouse and plants. We suggest that the Clot protein is an essential component of a glutathione redox system required for the final step in the biosynthetic pathway for drosopterins.
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- 2002
69. Role of FAM134 paralogues in endoplasmic reticulum remodeling, ER-phagy, and Collagen quality control
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Giorgia Di Lorenzo, Alessio Reggio, Carmine Cirillo, Elena Polishchuk, Adeela Hussain, Mariana Tellechea, Paolo Grumati, Alexandra Stolz, Marianna Esposito, Ivana Peluso, Antje K. Huebner, Christian A. Hübner, Viviana Buonomo, Carmine Settembre, Rayene Berkane, Ramachandra M. Bhaskara, Gerhard Hummer, Reggio, A., Buonomo, V., Berkane, R., Bhaskara, R. M., Tellechea, M., Peluso, I., Polishchuk, E., Di Lorenzo, G., Cirillo, C., Esposito, M., Hussain, A., Huebner, A. K., Hubner, C. A., Settembre, C., Hummer, G., Grumati, P., and Stolz, A.
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Proteomics ,Quality Control ,autophagy ,ER-phagy ,Cellular homeostasis ,Endoplasmic Reticulum ,Biochemistry ,Article ,03 medical and health sciences ,ddc:570 ,Genetics ,ddc:610 ,Fragmentation (cell biology) ,Receptor ,ER‐phagy ,Molecular Biology ,Membrane Protein ,030304 developmental biology ,Organelles ,0303 health sciences ,Chemistry ,Endoplasmic reticulum ,030302 biochemistry & molecular biology ,Autophagy ,Membrane Proteins ,Articles ,FAM134 ,Cell biology ,Cell culture ,Unfolded protein response ,ER stre ,Autophagy & Cell Death ,Collagen ,ER stress ,Flux (metabolism) - Abstract
Degradation of the endoplasmic reticulum (ER) via selective autophagy (ER‐phagy) is vital for cellular homeostasis. We identify FAM134A/RETREG2 and FAM134C/RETREG3 as ER‐phagy receptors, which predominantly exist in an inactive state under basal conditions. Upon autophagy induction and ER stress signal, they can induce significant ER fragmentation and subsequent lysosomal degradation. FAM134A, FAM134B/RETREG1, and FAM134C are essential for maintaining ER morphology in a LC3‐interacting region (LIR)‐dependent manner. Overexpression of any FAM134 paralogue has the capacity to significantly augment the general ER‐phagy flux upon starvation or ER‐stress. Global proteomic analysis of FAM134 overexpressing and knockout cell lines reveals several protein clusters that are distinctly regulated by each of the FAM134 paralogues as well as a cluster of commonly regulated ER‐resident proteins. Utilizing pro‐Collagen I, as a shared ER‐phagy substrate, we observe that FAM134A acts in a LIR‐independent manner and compensates for the loss of FAM134B and FAM134C, respectively. FAM134C instead is unable to compensate for the loss of its paralogues. Taken together, our data show that FAM134 paralogues contribute to common and unique ER‐phagy pathways., Selective degradation of the ER is essential to maintain ER homeostasis. This study characterizes FAM134A and FAM134C as ER‐phagy receptors involved in Collagen quality control and identifies protein clusters differentially regulated by FAM134 paralogues.
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- 2021
70. PD-L1 Dysregulation in COVID-19 Patients
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Francesco Sabbatino, Valeria Conti, Gianluigi Franci, Carmine Sellitto, Valentina Manzo, Pasquale Pagliano, Emanuela De Bellis, Alfonso Masullo, Francesco Antonio Salzano, Alessandro Caputo, Ilaria Peluso, Pio Zeppa, Giosuè Scognamiglio, Giuseppe Greco, Carla Zannella, Michele Ciccarelli, Claudia Cicala, Carmine Vecchione, Amelia Filippelli, Stefano Pepe, Sabbatino, F., Conti, V., Franci, G., Sellitto, C., Manzo, V., Pagliano, P., De Bellis, E., Masullo, A., Salzano, F. A., Caputo, A., Peluso, I., Zeppa, P., Scognamiglio, G., Greco, G., Zannella, C., Ciccarelli, M., Cicala, C., Vecchione, C., Filippelli, A., and Pepe, S.
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Adult ,Male ,PD-L1 ,0301 basic medicine ,Immunology ,B7-H1 Antigen ,Pathogenesis ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Downregulation and upregulation ,Humans ,Immunology and Allergy ,Medicine ,Lung ,Aged ,Original Research ,Aged, 80 and over ,Innate immune system ,biology ,SARS-CoV-2 ,business.industry ,immune checkpoint molecule ,COVID-19 ,Epithelial Cells ,RC581-607 ,Middle Aged ,Acquired immune system ,adaptive immune response ,Up-Regulation ,030104 developmental biology ,immune checkpoint molecules ,030220 oncology & carcinogenesis ,Leukocytes, Mononuclear ,innate immune response ,biology.protein ,Female ,ARDS ,prognosis ,Sample collection ,Immunologic diseases. Allergy ,business ,prognosi - Abstract
The COVID-19 pandemic has reached direct and indirect medical and social consequences with a subset of patients who rapidly worsen and die from severe-critical manifestations. As a result, there is still an urgent need to identify prognostic biomarkers and effective therapeutic approaches. Severe-critical manifestations of COVID-19 are caused by a dysregulated immune response. Immune checkpoint molecules such as Programmed death-1 (PD-1) and its ligand programmed death-ligand 1 (PD-L1) play an important role in regulating the host immune response and several lines of evidence underly the role of PD-1 modulation in COVID-19. Here, by analyzing blood sample collection from both hospitalized COVID-19 patients and healthy donors, as well as levels of PD-L1 RNA expression in a variety of model systems of SARS-CoV-2, including in vitro tissue cultures, ex-vivo infections of primary epithelial cells and biological samples obtained from tissue biopsies and blood sample collection of COVID-19 and healthy individuals, we demonstrate that serum levels of PD-L1 have a prognostic role in COVID-19 patients and that PD-L1 dysregulation is associated to COVID-19 pathogenesis. Specifically, PD-L1 upregulation is induced by SARS-CoV-2 in infected epithelial cells and is dysregulated in several types of immune cells of COVID-19 patients including monocytes, neutrophils, gamma delta T cells and CD4+ T cells. These results have clinical significance since highlighted the potential role of PD-1/PD-L1 axis in COVID-19, suggest a prognostic role of PD-L1 and provide a further rationale to implement novel clinical studies in COVID-19 patients with PD-1/PD-L1 inhibitors.
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- 2021
71. Mediterranean Diet for the Prevention of Gestational Diabetes in the Covid-19 Era: Implications of Il-6 In Diabesity
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Antonio Schiattarella, Elisabetta Toti, Ilaria Peluso, Maddalena Morlando, Anna Raguzzini, Anna Lucia Fedullo, Pasquale De Franciscis, Fedullo, A. L., Schiattarella, A., Morlando, M., Raguzzini, A., Toti, E., De Franciscis, P., and Peluso, I.
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Mediterranean diet ,Review ,Diet, Mediterranean ,lcsh:Chemistry ,chemistry.chemical_compound ,0302 clinical medicine ,Pregnancy ,oxidative stress ,plant foods ,030212 general & internal medicine ,lcsh:QH301-705.5 ,Spectroscopy ,chemistry.chemical_classification ,nutraceuticals ,biology ,General Medicine ,Plant food ,Gestational Weight Gain ,Computer Science Applications ,Gestational diabetes ,Female ,Nutraceutical ,Polyunsaturated fatty acid ,Human ,medicine.medical_specialty ,030209 endocrinology & metabolism ,Catalysis ,Inorganic Chemistry ,03 medical and health sciences ,Insulin resistance ,Diabetes mellitus ,Internal medicine ,medicine ,Animals ,Humans ,Obesity ,Physical and Theoretical Chemistry ,cardio-diabesity ,Interleukin 6 ,Molecular Biology ,Life Style ,business.industry ,Animal ,Interleukin-6 ,Organic Chemistry ,COVID-19 ,medicine.disease ,Diabetes, Gestational ,Endocrinology ,chemistry ,lcsh:Biology (General) ,lcsh:QD1-999 ,inflammation ,biology.protein ,Oxidative stre ,Glycated hemoglobin ,business - Abstract
The aim of this review is to highlight the influence of the Mediterranean Diet (MedDiet) on Gestational Diabetes Mellitus (GDM) and Gestational Weight Gain (GWG) during the COVID-19 pandemic era and the specific role of interleukin (IL)-6 in diabesity. It is known that diabetes, high body mass index, high glycated hemoglobin and raised serum IL-6 levels are predictive of poor outcomes in coronavirus disease 2019 (COVID-19). The immunopathological mechanisms of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection include rising levels of several cytokines and in particular IL-6. The latter is associated with hyperglycemia and insulin resistance and could be useful for predicting the development of GDM. Rich in omega-3 polyunsaturated fatty acids, vitamins, and minerals, MedDiet improves the immune system and could modulate IL-6, C reactive protein and Nuclear Factor (NF)-κB. Moreover, polyphenols could modulate microbiota composition, inhibit the NF-κB pathway, lower IL-6, and upregulate antioxidant enzymes. Finally, adhering to the MedDiet prior to and during pregnancy could have a protective effect, reducing GWG and the risk of GDM, as well as improving the immune response to viral infections such as COVID-19.
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- 2021
72. Plant-Derived Bioactives and Oxidative Stress-Related Disorders: A Key Trend towards Healthy Aging and Longevity Promotion
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Abhay Prakash Mishra, Elena Azzini, Maria Magdalena Quetglas-Llabrés, Ilaria Peluso, Natália Martins, Marc El Beyrouthy, Youssef El Rayess, Nanjangud V. Anil Kumar, Elena Maria Varoni, Patrick Valere Tsouh Fokou, Paolo Zucca, Mehdi Sharifi-Rad, William N. Setzer, Bahare Salehi, Marcello Iriti, Letizia Polito, Manisha Nigam, Leticia M. Estevinho, Javad Sharifi-Rad, Luciana Dini, Miquel Martorell, Antoni Sureda, Jovana Rajkovic, Elisa Panzarini, Instituto de Investigação e Inovação em Saúde, Salehi B., Azzini E., Zucca P., Varoni E.M., Kumar N.V.A., Dini L., Panzarini E., Rajkovic J., Fokou P.V.T., Peluso I., Mishra A.P., Nigam M., Rayess Y.E., Beyrouthy M.E., Setzer W.N., Polito L., Iriti M., Sureda A., Quetglas-Llabres M.M., Martorell M., Martins N., Sharifi-Rad M., Estevinho L.M., and Sharifi-Rad J.
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0301 basic medicine ,Bioactive molecules ,phenolic compounds ,Disease ,Bioinformatics ,medicine.disease_cause ,lcsh:Technology ,lcsh:Chemistry ,0302 clinical medicine ,Promotion (rank) ,Bioactive molecule ,oxidative stress ,General Materials Science ,Medicinal plants ,lcsh:QH301-705.5 ,Instrumentation ,media_common ,Chronic disorder ,Fluid Flow and Transfer Processes ,education.field_of_study ,Medicinal plant ,General Engineering ,Longevity ,lcsh:QC1-999 ,health maintenance ,3. Good health ,Computer Science Applications ,antioxidants ,030220 oncology & carcinogenesis ,Antioxidant ,media_common.quotation_subject ,Population ,Biology ,03 medical and health sciences ,longevity ,bioactive molecules ,medicine ,Healthy aging ,education ,lcsh:T ,Process Chemistry and Technology ,030104 developmental biology ,lcsh:Biology (General) ,lcsh:QD1-999 ,lcsh:TA1-2040 ,Oxidative stre ,lcsh:Engineering (General). Civil engineering (General) ,chronic disorders ,lcsh:Physics ,Oxidative stress ,medicinal plants - Abstract
Plants and their corresponding botanical preparations have been used for centuries due to their remarkable potential in both the treatment and prevention of oxidative stress-related disorders. Aging and aging-related diseases, like cardiovascular disease, cancer, diabetes, and neurodegenerative disorders, which have increased exponentially, are intrinsically related with redox imbalance and oxidative stress. Hundreds of biologically active constituents are present in each whole plant matrix, providing promissory bioactive effects for human beings. Indeed, the worldwide population has devoted increased attention and preference for the use of medicinal plants for healthy aging and longevity promotion. In fact, plant-derived bioactives present a broad spectrum of biological effects, and their antioxidant, anti-inflammatory, and, more recently, anti-aging effects, are considered to be a hot topic among the medical and scientific communities. Nonetheless, despite the numerous biological effects, it should not be forgotten that some bioactive molecules are prone to oxidation and can even exert pro-oxidant effects. In this sense, the objective of the present review is to provide a detailed overview of plant-derived bioactives in age-related disorders. Specifically, the role of phytochemicals as antioxidants and pro-oxidant agents is carefully addressed, as is their therapeutic relevance in longevity, aging-related disorders, and healthy-aging promotion. Finally, an eye-opening look into the overall evidence of plant compounds related to longevity is presented., N. Martins thank to the Portuguese Foundation for Science and Technology (FCT-Portugal) for Strategic project ref. UID/BIM/04293/2013 and NORTE2020-Programa Operacional Regional do Norte (NORTE-01-0145-FEDER-000012). A. Sureda was supported by Instituto de Salud Carlos III, Grant Number: CIBEROBN CB12/03/30038. Also, this work was supported by CONICYT PIA/APOYO CCTE AFB170007.
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- 2020
73. RNAi Triggered by Symmetrically Transcribed Transgenes in Drosophila melanogaster
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Ennio Giordano, Ivana Peluso, Maria Furia, Rosaria Rendina, Giordano, Ennio, Rendina, R., Peluso, I., and Furia, Maria
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Transcription, Genetic ,Biology ,chemistry.chemical_compound ,RNA interference ,Transcription (biology) ,Genetics ,Animals ,Drosophila Proteins ,Gene silencing ,Gene Silencing ,Transgenes ,Cloning, Molecular ,Gene ,Crosses, Genetic ,Hydro-Lyases ,RNA, Double-Stranded ,Regulation of gene expression ,fungi ,Nuclear Proteins ,RNA-Binding Proteins ,biology.organism_classification ,Drosophila melanogaster ,Gene Expression Regulation ,chemistry ,RNAi ,Insect Proteins ,Female ,Drosophila ,covergent trasncription ,vector ,Drosophila Protein ,DNA ,Research Article - Abstract
Specific silencing of target genes can be induced in a variety of organisms by providing homologous double-stranded RNA molecules. In vivo, these molecules can be generated either by transcription of sequences having an inverted-repeat (IR) configuration or by simultaneous transcription of sense-antisense strands. Since IR constructs are difficult to prepare and can stimulate genomic rearrangements, we investigated the silencing potential of symmetrically transcribed sequences. We report that Drosophila transgenes whose sense-antisense transcription was driven by two convergent arrays of Gal4-dependent UAS sequences can induce specific, dominant, and heritable repression of target genes. This effect is not dependent on a mechanism based on homology-dependent DNA/DNA interactions, but is directly triggered by transcriptional activation and is accompanied by specific depletion of the endogenous target RNA. Tissue-specific induction of these transgenes restricts the target gene silencing to selected body domains, and spreading phenomena described in other cases of post-transcriptional gene silencing (PTGS) were not observed. In addition to providing an additional tool useful for Drosophila functional genomic analysis, these results add further strength to the view that events of sense-antisense transcription may readily account for some, if not all, PTGS-cosuppression phenomena and can potentially play a relevant role in gene regulation.
- Published
- 2002
74. minifly, A Drosophila Gene Required for Ribosome Biogenesis
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Ennio Giordano, Ivana Peluso, Stefania Senger, Maria Furia, Giordano, Ennio, Peluso, I., Senger, S., and Furia, Maria
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Mutant ,Molecular Sequence Data ,Genes, Insect ,medicine.disease_cause ,snoRNA ,Dyskerin ,medicine ,Gene family ,Animals ,Drosophila Proteins ,Humans ,Amino Acid Sequence ,Small nucleolar RNA ,rRNA ,nucleolus ,RNA Processing, Post-Transcriptional ,Gene ,Hydro-Lyases ,DNA Primers ,Genetics ,Mutation ,biology ,Base Sequence ,Sequence Homology, Amino Acid ,Gene Expression Regulation, Developmental ,Nuclear Proteins ,RNA-Binding Proteins ,Cell Biology ,biology.organism_classification ,Rats ,Drosophila melanogaster ,Phenotype ,ribosome ,Insect Proteins ,Nucleic Acid Conformation ,RNA ,Drosophila ,Female ,Ribosomes ,Drosophila Protein ,Regular Articles - Abstract
We report here the genetic, molecular, and functional characterization of the Drosophila melanogaster minifly (mfl) gene. Genetic analysis shows that mfl is essential for Drosophila viability and fertility. While P-element induced total loss-of-function mutations cause lethality, mfl partial loss-of-function mutations cause pleiotropic defects, such as extreme reduction of body size, developmental delay, hatched abdominal cuticle, and reduced female fertility. Morphological abnormalities characteristic of apoptosis are found in the ovaries, and a proportion of eggs laid by mfl mutant females degenerates during embryogenesis. We show that mfl encodes an ubiquitous nucleolar protein that plays a central role in ribosomal RNA processing and pseudouridylation, whose known eukaryotic homologues are yeast Cfb5p, rat NAP57 and human dyskerin, encoded by the gene responsible for the X-linked dyskeratosis congenita disease. mfl genetic analysis represents the first in vivo functional characterization of a member of this highly conserved gene family from higher eukaryotes. In addition, we report that mfl hosts an intron encoded box H/ACA snoRNA gene, the first member of this class of snoRNAs identified so far from Drosophila.
- Published
- 1999
75. Molecular diagnosis of usher syndrome: application of two different next generation sequencing-based procedures
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Emmanouil Athanasakis, Ivana Peluso, Angela D'Eustacchio, Vincenzo Nigro, Antonella Fabretto, Carmela Ziviello, Nienke Wieskamp, Danilo Licastro, Margherita Mutarelli, Francesca Simonelli, Paolo Gasparini, Rossella Rispoli, F. d’Amico, Kornelia Neveling, Diego Vozzi, Mariateresa Pizzo, Hans Scheffer, Sandro Banfi, Licastro, Danilo, Mutarelli, Margherita, Peluso, Ivana, Neveling, Kornelia, Wieskamp, Nienke, Rispoli, Rossella, Vozzi, Diego, Athanasakis, Emmanouil, D'Eustacchio, Angela, Pizzo, Mariateresa, D'Amico, Francesca, Ziviello, Carmela, Simonelli, Francesca, Fabretto, Antonella, Scheffer, Han, Gasparini, Paolo, Banfi, Sandro, Nigro, Vincenzo, Licastro, D, Mutarelli, M, Peluso, I, Neveling, K, Wieskamp, N, Rispoli, R, Vozzi, D, Athanasakis, E, D'Eustacchio, A, Pizzo, M, D'Amico, F, Ziviello, C, Fabretto, A, Scheffer, H, and Gasparini, P
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Genetics and Molecular Biology (all) ,Genetic Screens ,Gene Identification and Analysis ,lcsh:Medicine ,Pilot Projects ,Biochemistry ,Genome Databases ,Exome ,Genome Sequencing ,lcsh:Science ,Child ,Exome sequencing ,Genetics ,0303 health sciences ,Multidisciplinary ,Massive parallel sequencing ,Genome ,Medicine (all) ,030305 genetics & heredity ,High-Throughput Nucleotide Sequencing ,Genomics ,3. Good health ,Molecular Diagnostic Techniques ,Child, Preschool ,Medicine ,Usher Syndrome ,Genome, Human ,Humans ,Sequence Analysis, DNA ,Usher Syndromes ,Agricultural and Biological Sciences (all) ,Biochemistry, Genetics and Molecular Biology (all) ,Sequence Analysis ,Research Article ,Human ,Molecular Diagnostic Technique ,Sequence Databases ,Genetic Counseling ,Biology ,DNA sequencing ,Genomic disorders and inherited multi-system disorders DCN MP - Plasticity and memory [IGMD 3] ,Genomic disorders and inherited multi-system disorders [IGMD 3] ,Molecular Genetics ,03 medical and health sciences ,Pilot Project ,Genetic Testing ,Preschool ,Genotyping ,030304 developmental biology ,Clinical Genetics ,lcsh:R ,Personalized Medicine ,Human Genetics ,DNA ,Molecular diagnostics ,Otorhinolaryngology ,Genetics of Disease ,Mutation Databases ,Human genome ,lcsh:Q - Abstract
Contains fulltext : 108716.pdf (Publisher’s version ) (Open Access) Usher syndrome (USH) is a clinically and genetically heterogeneous disorder characterized by visual and hearing impairments. Clinically, it is subdivided into three subclasses with nine genes identified so far. In the present study, we investigated whether the currently available Next Generation Sequencing (NGS) technologies are already suitable for molecular diagnostics of USH. We analyzed a total of 12 patients, most of which were negative for previously described mutations in known USH genes upon primer extension-based microarray genotyping. We enriched the NGS template either by whole exome capture or by Long-PCR of the known USH genes. The main NGS sequencing platforms were used: SOLiD for whole exome sequencing, Illumina (Genome Analyzer II) and Roche 454 (GS FLX) for the Long-PCR sequencing. Long-PCR targeting was more efficient with up to 94% of USH gene regions displaying an overall coverage higher than 25x, whereas whole exome sequencing yielded a similar coverage for only 50% of those regions. Overall this integrated analysis led to the identification of 11 novel sequence variations in USH genes (2 homozygous and 9 heterozygous) out of 18 detected. However, at least two cases were not genetically solved. Our result highlights the current limitations in the diagnostic use of NGS for USH patients. The limit for whole exome sequencing is linked to the need of a strong coverage and to the correct interpretation of sequence variations with a non obvious, pathogenic role, whereas the targeted approach suffers from the high genetic heterogeneity of USH that may be also caused by the presence of additional causative genes yet to be identified.
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- 2012
76. A high-resolution anatomical atlas of the transcriptome in the mouse embryo
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Graciana Diez-Roux, Sandro Banfi, Marc Sultan, Lars Geffers, Santosh Anand, David Rozado, Alon Magen, Elena Canidio, Massimiliano Pagani, Ivana Peluso, Nathalie Lin-Marq, Muriel Koch, Marchesa Bilio, Immacolata Cantiello, Roberta Verde, Cristian De Masi, Salvatore A Bianchi, Juliette Cicchini, Elodie Perroud, Shprese Mehmeti, Emilie Dagand, Sabine Schrinner, Asja Nürnberger, Katja Schmidt, Katja Metz, Christina Zwingmann, Norbert Brieske, Cindy Springer, Ana Martinez Hernandez, Sarah Herzog, Frauke Grabbe, Cornelia Sieverding, Barbara Fischer, Kathrin Schrader, Maren Brockmeyer, Sarah Dettmer, Christin Helbig, Violaine Alunni, Marie-Annick Battaini, Carole Mura, Charlotte N Henrichsen, Raquel Garcia-Lopez, Diego Echevarria, Eduardo Puelles, Elena Garcia-Calero, Stefan Kruse, Markus Uhr, Christine Kauck, Guangjie Feng, Nestor Milyaev, Chuang Kee Ong, Lalit Kumar, MeiSze Lam, Colin A Semple, Attila Gyenesei, Stefan Mundlos, Uwe Radelof, Hans Lehrach, Paolo Sarmientos, Alexandre Reymond, Duncan R Davidson, Pascal Dollé, Stylianos E Antonarakis, Marie-Laure Yaspo, Salvador Martinez, Richard A Baldock, Gregor Eichele, Andrea Ballabio, Telethon Institute for Genetics and Medicine, Telethon Institute, Max Planck Institute for Molecular Genetics (MPIMG), Max-Planck-Gesellschaft, Genes and Behavior Department [Göttingen], Max Planck Institute for Biophysical Chemistry (MPI-BPC), Max-Planck-Gesellschaft-Max-Planck-Gesellschaft, Primm, Department of Genetic Medicine and Development [Geneva], Université de Genève (UNIGE), Institut Clinique de la Souris (ICS), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Center for Integrative Genomics - Institute of Bioinformatics, Génopode (CIG), Swiss Institute of Bioinformatics [Lausanne] (SIB), Université de Lausanne (UNIL)-Université de Lausanne (UNIL), Experimental Embryology Lab, Universidad Miguel Hernández [Elche] (UMH)-Instituto de Neurociencias, ORGARAT, Human Genetics Unit, Medical Research Council, Deutsches Ressourcenzentrum für Genomforschung (RZPD), Deutsches Ressourcenzentrum für Genomforschung, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Medical Genetics, Università degli studi di Napoli Federico II, Department of Molecular and Human Genetics, Baylor College of Medicine (BCM), Baylor University-Baylor University, Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital [Houston, USA], This work was supported by the EC VI Framework Programme contract number LSHG-CT-2004-512003. The authors also acknowledge the support of: the Italian Telethon Foundation (AB, SB, and GD-R), the Swiss National Science Foundation (AR and SEA), the Max Planck Society (GE, M-LY, HL), MRC (RB, DD), Association pour la Recherche sur le Cancer (PD), and Ingenio 2010 MEC-CONSOLIDER CSD2007-00023, DIGESIC-MEC BFU2008-00588, CIBERSAM/ISCIII (SM)., Université de Genève = University of Geneva (UNIGE), Université de Lausanne = University of Lausanne (UNIL)-Université de Lausanne = University of Lausanne (UNIL), University of Naples Federico II = Università degli studi di Napoli Federico II, Autard, Delphine, Diez Roux, G, Banfi, Sandro, Sultan, M, Geffers, L, Anand, S, Rozado, D, Magen, A, Canidio, E, Pagani, M, Peluso, I, Lin Marq, N, Koch, M, Bilio, M, Cantiello, I, Verde, R, De Masi, C, Bianchi, Sa, Cicchini, J, Perroud, E, Mehmeti, S, Dagand, E, Schrinner, S, Nürnberger, A, Schmidt, K, Metz, K, Zwingmann, C, Brieske, N, Springer, C, Martinez Hernandez, A, Herzog, S, Grabbe, F, Sieverding, C, Fischer, B, Schrader, K, Bürsing, M, Schubert, S, Helbig, C, Alunni, V, Battaini, Ma, Mura, C, Henrichsen, Cn, Garcia Lopez, R, Echevarria, D, Puelles, E, Garcia Calero, E, Kruse, S, Uhr, M, Kauck, C, Feng, G, Milyaev, N, Ong, Ck, Kumar, L, Lam, M, Semple, Ca, Gyenesei, A, Mundlos, S, Radelof, U, Lehrach, H, Sarmientos, P, Reymond, A, Davidson, Dr, Dollé, P, Antonarakis, Se, Yaspo, Ml, Martinez, M, Baldock, Ra, Eichele, G, Ballabio, A., Banfi, S, Reymond, R, Martinez, S, Ballabio, Andrea, and Reymond, Alexandre
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Transcriptome ,Mice ,0302 clinical medicine ,Databases, Genetic ,Gene expression ,Animals ,Atlases as Topic ,Embryo, Mammalian ,Gene Expression Profiling ,Internet ,Mice/anatomy & histology ,Mice/embryology ,Mice, Inbred C57BL ,Organ Specificity ,ddc:576.5 ,MESH: Animals ,Biology (General) ,[SDV.BDD]Life Sciences [q-bio]/Development Biology ,MESH: Databases, Genetic ,MESH: Organ Specificity ,Genetics ,0303 health sciences ,Agricultural and Biological Sciences(all) ,General Neuroscience ,Wnt signaling pathway ,Genetics and Genomics/Gene Expression ,Genome project ,MESH: Internet ,General Agricultural and Biological Sciences ,Research Article ,Genetics and Genomics/Animal Genetics ,QH301-705.5 ,Neuroscience(all) ,education ,MESH: Atlases as Topic ,In situ hybridization ,Computational biology ,Biology ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,MESH: Gene Expression Profiling ,Mice/anatomy & histology/embryology/genetics ,MESH: Mice, Inbred C57BL ,Immunology and Microbiology(all) ,microRNA ,[SDV.BDD] Life Sciences [q-bio]/Development Biology ,Gene ,MESH: Mice ,030304 developmental biology ,General Immunology and Microbiology ,Biochemistry, Genetics and Molecular Biology(all) ,MESH: Embryo, Mammalian ,Gene expression profiling ,Genetics and Genomics/Genome Projects ,030217 neurology & neurosurgery ,Developmental Biology - Abstract
The manuscript describes the “digital transcriptome atlas” of the developing mouse embryo, a powerful resource to determine co-expression of genes, to identify cell populations and lineages and to identify functional associations between genes relevant to development and disease., Ascertaining when and where genes are expressed is of crucial importance to understanding or predicting the physiological role of genes and proteins and how they interact to form the complex networks that underlie organ development and function. It is, therefore, crucial to determine on a genome-wide level, the spatio-temporal gene expression profiles at cellular resolution. This information is provided by colorimetric RNA in situ hybridization that can elucidate expression of genes in their native context and does so at cellular resolution. We generated what is to our knowledge the first genome-wide transcriptome atlas by RNA in situ hybridization of an entire mammalian organism, the developing mouse at embryonic day 14.5. This digital transcriptome atlas, the Eurexpress atlas (http://www.eurexpress.org), consists of a searchable database of annotated images that can be interactively viewed. We generated anatomy-based expression profiles for over 18,000 coding genes and over 400 microRNAs. We identified 1,002 tissue-specific genes that are a source of novel tissue-specific markers for 37 different anatomical structures. The quality and the resolution of the data revealed novel molecular domains for several developing structures, such as the telencephalon, a novel organization for the hypothalamus, and insight on the Wnt network involved in renal epithelial differentiation during kidney development. The digital transcriptome atlas is a powerful resource to determine co-expression of genes, to identify cell populations and lineages, and to identify functional associations between genes relevant to development and disease., Author Summary In situ hybridization (ISH) can be used to visualize gene expression in cells and tissues in their native context. High-throughput ISH using nonradioactive RNA probes allowed the Eurexpress consortium to generate a comprehensive, interactive, and freely accessible digital gene expression atlas, the Eurexpress transcriptome atlas (http://www.eurexpress.org), of the E14.5 mouse embryo. Expression data for over 15,000 genes were annotated for hundreds of anatomical structures, thus allowing us to systematically identify tissue-specific and tissue-overlapping gene networks. We illustrate the value of the Eurexpress atlas by finding novel regional subdivisions in the developing brain. We also use the transcriptome atlas to allocate specific components of the complex Wnt signaling pathway to kidney development, and we identify regionally expressed genes in liver that may be markers of hematopoietic stem cell differentiation.
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- 2011
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77. miRNeye: a microRNA expression atlas of the mouse eye
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Vincenzo A. Gennarino, Sandro Banfi, Marianthi Karali, Marchesa Bilio, Roberta Verde, Ivana Peluso, Pascal Dollé, Giampiero Lago, Karali, M, Peluso, I, Gennarino, Va, Bilio, M, Verde, R, Lago, G, Dollé, P, Banfi, Sandro, Telethon Institute for Genetics and Medicine, Telethon Institute, Department of Molecular and Human Genetics, Baylor College of Medicine (BCM), Baylor University-Baylor University, Istituto di Chimica Biomolecolare (ICB), Centre National de la Recherche (CNR), Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), This work was supported by the European Community (EURExpress grant n. LSHG-CT-2004- 512003 and EVI-GENORET grant n. LSHG-CT-2005-512036) and the Fondazione Telethon. MK acknowledges financial support by a Marie Curie European Re-integration Grant (grant n. PERG03-GA-2008-231068)., and BMC, Ed.
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Time Factors ,Microarray ,genetic structures ,Iris ,Retinal Pigment Epithelium ,Proteomics ,Eye ,Cornea ,Mice ,0302 clinical medicine ,MESH: Gene Expression Regulation, Developmental ,Databases, Genetic ,MESH: Animals ,MESH: Organ Specificity ,MESH: Databases, Genetic ,In Situ Hybridization ,Genetics ,Regulation of gene expression ,0303 health sciences ,Gene Expression Regulation, Developmental ,MESH: Lens, Crystalline ,MESH: Retinal Pigment Epithelium ,medicine.anatomical_structure ,MESH: Internet ,Organ Specificity ,[SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,DNA microarray ,Biotechnology ,Research Article ,lcsh:QH426-470 ,lcsh:Biotechnology ,MESH: Eye ,In situ hybridization ,Computational biology ,Biology ,MESH: Ciliary Body ,03 medical and health sciences ,MESH: Gene Expression Profiling ,MESH: In Situ Hybridization ,MESH: Mice, Inbred C57BL ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,lcsh:TP248.13-248.65 ,microRNA ,Lens, Crystalline ,medicine ,Animals ,MESH: Mice ,030304 developmental biology ,Internet ,Retinal pigment epithelium ,Gene Expression Profiling ,MESH: Time Factors ,MESH: Cornea ,Ciliary Body ,MESH: Iris ,eye diseases ,Gene expression profiling ,Mice, Inbred C57BL ,MicroRNAs ,lcsh:Genetics ,030221 ophthalmology & optometry ,sense organs ,MESH: MicroRNAs - Abstract
Background MicroRNAs (miRNAs) are key regulators of biological processes. To define miRNA function in the eye, it is essential to determine a high-resolution profile of their spatial and temporal distribution. Results In this report, we present the first comprehensive survey of miRNA expression in ocular tissues, using both microarray and RNA in situ hybridization (ISH) procedures. We initially determined the expression profiles of miRNAs in the retina, lens, cornea and retinal pigment epithelium of the adult mouse eye by microarray. Each tissue exhibited notably distinct miRNA enrichment patterns and cluster analysis identified groups of miRNAs that showed predominant expression in specific ocular tissues or combinations of them. Next, we performed RNA ISH for over 220 miRNAs, including those showing the highest expression levels by microarray, and generated a high-resolution expression atlas of miRNAs in the developing and adult wild-type mouse eye, which is accessible in the form of a publicly available web database. We found that 122 miRNAs displayed restricted expression domains in the eye at different developmental stages, with the majority of them expressed in one or more cell layers of the neural retina. Conclusions This analysis revealed miRNAs with differential expression in ocular tissues and provided a detailed atlas of their tissue-specific distribution during development of the murine eye. The combination of the two approaches offers a valuable resource to decipher the contributions of specific miRNAs and miRNA clusters to the development of distinct ocular structures.
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- 2010
78. Biochemical characterization and homology modeling of a purine-specific ribonucleoside hydrolase from the archaeon Sulfolobus solfataricus: insights into mechanisms of protein stabilization
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Angelo Facchiano, Marina Porcelli, Iolanda Peluso, Anna Marabotti, Giovanna Cacciapuoti, Porcelli, Marina, Peluso, I, Marabotti, A, Facchiano, A, and Cacciapuoti, Giovanna
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Models, Molecular ,ved/biology.organism_classification_rank.species ,Molecular Sequence Data ,Biophysics ,Guanosine ,Biochemistry ,Genes, Archaeal ,Substrate Specificity ,chemistry.chemical_compound ,Catalytic Domain ,Hydrolase ,Enzyme Stability ,medicine ,Animals ,Homology modeling ,Amino Acid Sequence ,Cloning, Molecular ,Inosine ,Molecular Biology ,N-Glycosyl Hydrolases ,DNA Primers ,Crithidia fasciculata ,biology ,Base Sequence ,Sequence Homology, Amino Acid ,ved/biology ,Sulfolobus solfataricus ,Purine Nucleosides ,Ribonucleoside ,biology.organism_classification ,Recombinant Proteins ,Kinetics ,chemistry ,Thermodynamics ,Protein stabilization ,medicine.drug - Abstract
We report the biochemical and structural characterization of the purine-specific ribonucleoside hydrolase from the archaeon Sulfolobus solfataricus (SsIAG-NH). SsIAG-NH is a homodimer of 70 kDa specific for adenosine, guanosine and inosine. SsIAG-NH is highly thermophilic and is characterized by extreme thermodynamic stability (Tm, 107 °C), kinetic stability and remarkable resistance to guanidinium chloride-induced unfolding. A disulfide bond that, on the basis of SDS–PAGE is positioned intersubunits, plays an important role in thermal stability. SsIAG-NH shares 43% sequence identity with the homologous pyrimidine-specific nucleoside hydrolase from S. solfataricus (SsCU-NH). The comparative sequence alignment of SsIAG-NH, SsCU-NH, purine non-specific nucleoside hydrolase from Crithidia fasciculata and purine-specific nucleoside hydrolase from Trypanosoma vivax shows that, only few changes in the base pocket are responsible for different substrate specificity of two S. solfataricus enzymes. The structure of SsIAG-NH predicted by homology modeling allows us to infer the role of specific residues in substrate specificity and thermostability.
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- 2009
79. Identification and characterization of microRNAs expressed in the mouse eye
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Sandro Banfi, Ivana Peluso, Valeria Marigo, Marianthi Karali, Karali, M, Peluso, I, Marigo, V, and Banfi, Sandro
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Untranslated region ,retina ,genetic structures ,Databases, Factual ,Biology ,Retina ,Transcriptome ,Cornea ,Mice ,microRNA ,expression ,Gene expression ,Lens, Crystalline ,Animals ,Gene ,In Situ Hybridization, Fluorescence ,Genetics ,cDNA library ,RNA ,Gene Expression Regulation, Developmental ,Cell Differentiation ,eye diseases ,Cell biology ,MicroRNAs ,Microscopy, Fluorescence ,Eye development ,sense organs ,Oligonucleotide Probes - Abstract
PURPOSE. MicroRNAs (miRNAs) are a class of small, endogenous RNAs that negatively regulate gene expression post-transcriptionally by binding to target sites in the 3' untranslated region (UTR) of messenger RNAs. Although they have been found to regulate developmental and physiological processes in several organs and tissues, their role in the eye transcriptome is completely unknown. This study was conducted to gain understanding of their eye-related function in mammals, by looking for miRNAs significantly expressed in the mouse eye by means of high-resolution expression analysis. METHODS. The spatiotemporal localization of miRNAs was analyzed in the murine embryonic and postnatal eye by RNA in situ hybridization (ISH) using LNA-modified oligonucleotide probes. RESULTS. Seven miRNAs were expressed in the eye with diverse and partially overlapping patterns, which may reflect their role in controlling cell differentiation of the retina as well as of other ocular structures. Most eye-expressed miRNAs overlap with or are in the near vicinity of transcripts derived predominantly from eye cDNA libraries. We found that these transcripts share very similar cellular distribution with their corresponding miRNAs, suggesting that miRNAs may share common expression regulatory elements with their host genes. CONCLUSIONS. The data provide a detailed characterization of expression of eye-enriched miRNAs. Knowledge of the spatiotemporal distribution of miRNAs is an essential step toward the identification of their targets and eventually the elucidation of their biological role in eye development and function.
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- 2007
80. Functional analysis of regulatory elements controlling the expression of the ecdysone-regulated Drosophila ng-1 gene
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Ivana Peluso, Maria Furia, Stefania Crispi, Pier Paolo D'Avino, Ennio Giordano, Crispi, S., Giordano, Ennio, D'Avino, P. P., Peluso, I., and Furia, Maria
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Ecdysone ,Embryology ,Time Factors ,Transcription, Genetic ,Transgene ,media_common.quotation_subject ,Molecular Sequence Data ,Biology ,Animals, Genetically Modified ,chemistry.chemical_compound ,Sequence Homology, Nucleic Acid ,Gene expression ,Animals ,Drosophila Proteins ,Coding region ,Amino Acid Sequence ,Salivary Proteins and Peptides ,Metamorphosis ,Gene ,Transcription factor ,media_common ,Regulation of gene expression ,Genetics ,Base Sequence ,Models, Genetic ,Gene Expression Regulation, Developmental ,Nuclear Proteins ,Forkhead Transcription Factors ,Blotting, Northern ,beta-Galactosidase ,Drosophila melanogaster ,Lac Operon ,chemistry ,RNA ,Transcription Factors ,Developmental Biology - Abstract
The steroid hormone ecdysone controls multiple aspects of insect development, including larval moults and metamorphosis, and can induce specific genetic responses in different tissues. The definition of the molecular mechanisms able to mediate this tissue-specific responsiveness may greatly contribute to understanding how such an accurate genetic response is achieved. In this work we have identified, by transgenic analysis, the regulatory elements directing the expression of ng-1, an ecdysone-regulated Drosophila gene showing a highly specific developmental expression profile. Our results show that an ecdysone-responsive element located within the ng-1 coding region is necessary for high-level gene expression, whereas the gene's spatial and temporal expression profile is fully controlled by a distinct upstream regulatory region. This region binds a set of transcriptional factors, including the FKH regulatory protein, which can potentially modulate the ecdysone genetic regulated response.
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- 2001
81. Disruption of the IQSEC2 transcript in a female with X;autosome translocation t(X;20)(p11.2;q11.2) and a phenotype resembling X-linked infantile spasms (ISSX) syndrome
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David Chitayat, Ivana Peluso, Manuela Morleo, Brunella Franco, R. Marzella, Daniela Iaconis, Alessandra Renieri, Francesca Mari, M., Morleo, D., Iaconi, D., Chitayat, I., Peluso, R., Marzella, A., Renieri, F., Mari, Franco, Brunella, Morleo, M, Iaconis, D, Chitayat, D, Peluso, I, Marzella, R, Renieri, A, Mari, F, and Franco, B
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Genetics ,Cancer Research ,Candidate gene ,Oncogene ,Chromosomal translocation ,GTPase ,Biology ,Biochemistry ,Phenotype ,Hypsarrhythmia ,Oncology ,medicine ,Molecular Medicine ,Small GTPase ,medicine.symptom ,Molecular Biology ,Gene - Abstract
We report on a female patient with severe infantile spasms, profound global developmental arrest, hypsarrhythmia and severe mental retardation, associated with a de novo apparently balanced X;autosome translocation. Her neurological phenotype resembles that of X-linked infantile spasms (ISSX). Molecular study showed that the translocation disrupts a transcript involved in GTPases signalling, IQSEC2, mapped to the Xp11.22 region. Several genes involved in intracellular signalling pathways via Ras-homologous small GTPase have been implicated in X-linked neurological disorders. Expression studies revealed that the murine homolog of this transcript, Iqsec2, is highly expressed in the nervous system from the early stages of development. These data suggest that IQSEC2 could be considered a candidate gene for X-linked neurological disorders.
82. PAR level mediates the link between ROS and inflammatory response in patients with type 2 diabetes mellitus.
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Zampieri M, Karpach K, Salerno G, Raguzzini A, Barchetta I, Cimini FA, Dule S, De Matteis G, Zardo G, Borro M, Peluso I, Cavallo MG, and Reale A
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- Humans, Male, Female, Middle Aged, Cross-Sectional Studies, Poly Adenosine Diphosphate Ribose metabolism, Superoxide Dismutase-1 metabolism, Superoxide Dismutase-1 genetics, Glutathione Peroxidase GPX1, Glutathione Peroxidase metabolism, Glutathione Peroxidase blood, Biomarkers blood, Adult, Aged, Tumor Necrosis Factor-alpha metabolism, Tumor Necrosis Factor-alpha blood, Catalase metabolism, Catalase blood, Diabetes Mellitus, Type 2 metabolism, Oxidative Stress, Reactive Oxygen Species metabolism, Inflammation metabolism, Leukocytes, Mononuclear metabolism
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Background: Type 2 diabetes mellitus (T2DM) is characterized by disrupted glucose homeostasis and metabolic abnormalities, with oxidative stress and inflammation playing pivotal roles in its pathophysiology. Poly(ADP-ribosyl)ation (PARylation) is a post-translational process involving the addition of ADP-ribose polymers (PAR) to target proteins. While preclinical studies have implicated PARylation in the interplay between oxidative stress and inflammation in T2DM, direct clinical evidence in humans remains limited. This study investigates the relationship between oxidative stress, PARylation, and inflammatory response in T2DM patients., Methods: This cross-sectional investigation involved 61 T2DM patients and 48 controls. PAR levels were determined in peripheral blood cells (PBMC) by ELISA-based methodologies. Oxidative stress was assessed in plasma and PBMC. In plasma, we monitored reactive oxygen metabolites (d-ROMs) and ferric-reducing antioxidant power. In PBMC, we measured the expression of antioxidant enzymes SOD1, GPX1 and CAT by qPCR. Further, we evaluated the expression of inflammatory mediators such as IL6, TNF-α, CD68 and MCP1 by qPCR in PBMC., Results: T2DM patients exhibited elevated PAR levels in PBMC and increased d-ROMs in plasma. Positive associations were found between PAR levels and d-ROMs, suggesting a link between oxidative stress and altered PAR metabolism. Mediation analysis revealed that d-ROMs mediate the association between HbA1c levels and PAR, indicating oxidative stress as a potential driver of increased PARylation in T2DM. Furthermore, elevated PAR levels were found to be associated with increased expression of pro-inflammatory cytokines IL6 and TNF-α in the PBMC of T2DM patients., Conclusions: This study highlights that hyperactivation of PARylation is associated with poor glycemic control and the resultant oxidative stress in T2DM. The increase of PAR levels is correlated with the upregulation of key mediators of the inflammatory response. Further research is warranted to validate these findings and explore their clinical implications., Competing Interests: Declaration of competing interest We wish to confirm that there are no known conflicts of interest associated with this publication and there has been no significant financial support for this work that could have influenced its outcome., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
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- 2024
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83. Upper-Limb Muscle Fatigability in Para-Athletes Quantified as the Rate of Force Development in Rapid Contractions of Submaximal Amplitude.
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Boccia G, Brustio PR, Beratto L, Peluso I, Ferrara R, Munzi D, Toti E, Raguzzini A, Sciarra T, and Rainoldi A
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This study aimed to compare neuromuscular fatigability of the elbow flexors and extensors between athletes with amputation (AMP) and athletes with spinal cord injury (SCI) for maximum voluntary force (MVF) and rate of force development (RFD). We recruited 20 para-athletes among those participating at two training camps (2022) for Italian Paralympic veterans. Ten athletes with SCI (two with tetraplegia and eight with paraplegia) were compared to 10 athletes with amputation (above the knee, N = 3; below the knee, N = 6; forearm, N = 1). We quantified MVF, RFD at 50, 100, and 150 ms, and maximal RFD (RFDpeak) of elbow flexors and extensors before and after an incremental arm cranking to voluntary fatigue. We also measured the RFD scaling factor (RFD-SF), which is the linear relationship between peak force and peak RFD quantified in a series of ballistic contractions of submaximal amplitude. SCI showed lower levels of MVF and RFD in both muscle groups (all p values ≤ 0.045). Despite this, the decrease in MVF (Cohen's d = 0.425, p < 0.001) and RFDpeak (d = 0.424, p = 0.003) after the incremental test did not show any difference between pathological conditions. Overall, RFD at 50 ms showed the greatest decrease (d = 0.741, p < 0.001), RFD at 100 ms showed a small decrease (d = 0.382, p = 0.020), and RFD at 150 ms did not decrease ( p = 0.272). The RFD-SF decreased more in SCI than AMP ( p < 0.0001). Muscle fatigability impacted not only maximal force expressions but also the quickness of ballistic contractions of submaximal amplitude, particularly in SCI. This may affect various sports and daily living activities of wheelchair users. Early RFD (i.e., ≤50 ms) was notably affected by muscle fatigability.
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- 2024
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84. Neuroprotective and anti-inflammatory effects of curcumin in Alzheimer's disease: Targeting neuroinflammation strategies.
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Azzini E, Peña-Corona SI, Hernández-Parra H, Chandran D, Saleena LAK, Sawikr Y, Peluso I, Dhumal S, Kumar M, Leyva-Gómez G, Martorell M, Sharifi-Rad J, and Calina D
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- Humans, Animals, Neuroinflammatory Diseases drug therapy, Antioxidants pharmacology, Curcuma chemistry, Biological Availability, Curcumin pharmacology, Curcumin therapeutic use, Alzheimer Disease drug therapy, Neuroprotective Agents pharmacology, Anti-Inflammatory Agents pharmacology
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Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterized by the accumulation of amyloid-beta plaques and neurofibrillary tangles, leading to neuronal loss. Curcumin, a polyphenolic compound derived from Curcuma longa, has shown potential neuroprotective effects due to its anti-inflammatory and antioxidant properties. This review aims to synthesize current preclinical data on the anti-neuroinflammatory mechanisms of curcumin in the context of AD, addressing its pharmacokinetics, bioavailability, and potential as a therapeutic adjunct. An exhaustive literature search was conducted, focusing on recent studies within the last 10 years related to curcumin's impact on neuroinflammation and its neuroprotective role in AD. The review methodology included sourcing articles from specialized databases using specific medical subject headings terms to ensure precision and relevance. Curcumin demonstrates significant neuroprotective properties by modulating neuroinflammatory pathways, scavenging reactive oxygen species, and inhibiting the production of pro-inflammatory cytokines. Despite its potential, challenges remain regarding its limited bioavailability and the scarcity of comprehensive human clinical trials. Curcumin emerges as a promising therapeutic adjunct in AD due to its multimodal neuroprotective benefits. However, further research is required to overcome challenges related to bioavailability and to establish effective dosing regimens in human subjects. Developing novel delivery systems and formulations may enhance curcumin's therapeutic potential in AD treatment., (© 2024 John Wiley & Sons Ltd.)
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- 2024
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85. Optimizing wheelchair basketball lineups: A statistical approach to coaching strategies.
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Cavedon V, Zuccolotto P, Sandri M, Manisera M, Bernardi M, Peluso I, and Milanese C
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- Humans, Male, Adult, Basketball, Wheelchairs, Athletic Performance, Mentoring methods
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This study was designed to support the tactical decisions of wheelchair basketball (WB) coaches in identifying the best players to form winning lineups. Data related to a complete regular season of a top-level WB Championship were examined. By analyzing game-related statistics from the first round, two clusters were identified that accounted for approximately 35% of the total variance. Cluster 1 was composed of low-performing athletes, while Cluster 2 was composed of high-performing athletes. Based on data related to the second round of the Championship, we conducted a two-fold evaluation of the clusters identified in the first round with the team's net performance as the outcome variable. The results showed that teams where players belonging to Cluster 2 had played more time during the second round of the championship were also those with the better team performance (R-squared = 0.48, p = 0.035), while increasing the playing time for players from Classes III and IV does not necessarily improve team performance (r2 = -0.14, p = 0.59). These results of the present study suggest that a collaborative approach between coaches and data scientists would significantly advance this Paralympic sport., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Cavedon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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- 2024
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86. Overlap of orthorexia, eating attitude and psychological distress in some Italian and Spanish university students.
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Aiello P, Toti E, Villaño D, Raguzzini A, and Peluso I
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Background: Orthorexia nervosa (ON) is the persistent concern of maintaining the self-imposed diet to improve one's health. Many factors have been associated to ON in univ-ersity students., Aim: To assess the prevalence of ON in Italian and Spanish university students in relation to eating attitude and psychological distress, and the possible overlaps between ON (evaluated with different scored questionnaires from the originally proposed ORTO-15), distress and risk of eating disorders., Methods: This study was carried out on 160 students recruited at La Sapienza University of Rome and at the Catholic University of Murcia. Questionnaires were administered to evaluate ON (ORTO-15 and sub-scores), body concerns (Multidimensional Body-Self Relations Questionnaire, MBSRQ, and Body Uneasiness test, BUT), psychological distress (Kessler Psychological Distress Scale, K10), physical activity (International Physical Activity Questionnaire, IPAQ), eating attitude (Eating Attitudes Test, EAT-26) and malnutrition (Starvation Symptom Inventory, SSI). Sex differences, within the same country, and differences between Italian and Spanish students, within the same sex, were evaluated., Results: The ORTO-15 positive subjects, assessed with the originally proposed cut-off, were above 70% in both Italian and Spanish students, with a higher prevalence in the Spanish sample (Italian females 76.3%, Italian males 70.7%; Spanish females 97.0%, Spanish males 96.3%). According to ORTO-7, about 30% of Italian and 48% of Spanish students were positive to ON with no significant sex differences. When excluding students underweight (UW), overweight (OW) or obese (OB), as well as those potentially at risk of eating disorders or presenting mild, moderate and severe distress, in the resultant normal weight (NW)-K10
neg -EAT-26neg subgroup, we did not find many correlations observed in the whole sample, including those between ORTO scores and BUT, SSI, Total MBSRQ and some of its components. Moreover, ORTO-7 resulted in the only ON score unrelated with Body Mass Index, MBSRQ components and IPAQ-assessed intense activity, in the NW-K10neg -EAT-26neg subgroup. After this sort of "exclusion diagnosis", the prevalence of ON of these students on the overall sample resulted in 16.9%, 12.2%, 15.2% and 25.9% for Italian females, Italian males, Spanish females and Spanish males, respectively., Conclusion: In some university students ON could be a symptom of other conditions related to body image concerns and distress, as well as to high physical activity and appearance, fitness, health or illness orientation (from MBSRQ). However, ORTO-7 became independent from these confounding variables, after the exclusion of UW, OW, OB and students positive to EAT-26 and K10, suggesting the possibility of identifying orthorexic subjects with this specific questionnaire., Competing Interests: Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article., (©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.)- Published
- 2022
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87. Sporting activity does not fully prevent bone demineralization at the impaired hip in athletes with amputation.
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Cavedon V, Sandri M, Peluso I, Zancanaro C, and Milanese C
- Abstract
There is lack of information about bone mineralization at the lumbar spine and bilateral hips of athletes with unilateral lower limb amputation. The present study assessed for the first time the areal bone mineral density at the lumbar spine and at the hip of the able and impaired leg by means of Dual-Energy X-Ray Absorptiometry using a large sample ( N = 40) of male athletes. Results showed that bone demineralization in athletes with unilateral lower limb amputation is found at the impaired hip but not at the lumbar spine and may therefore be site-specific. The extent of hip demineralization was influenced by the level of amputation, with about 80% of athletes with above knee amputation and 10% of athletes with below knee amputation showing areal bone mineral density below the expected range for age. Nevertheless, a reduced percentage of fat mass and a lower fat-to-lean mass ratio in the residual impaired leg as well as a greater amount of weekly training was positively associated with bone mineralization at the impaired hip (partial correlation coefficients = 0.377-0.525, p = 0.040-0.003). Results showed that participation in adapted sport has a positive effect on bone health in athletes with unilateral lower limb amputation but is not sufficient to maintain adequate levels of bone mineralization at the impaired hip in athletes with above-knee amputation. Accordingly, physical conditioners should consider implementing sporting programs, according to the severity of the impairment, aimed at improving bone mineralization at the impaired hip and improve body composition in the residual impaired leg., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Cavedon, Sandri, Peluso, Zancanaro and Milanese.)
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- 2022
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88. Body Composition and Metabolic Status of Italian and Spanish University Students: Relationship with Fruit and Vegetable Consumption.
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Aiello P, Peluso I, Di Giacomo S, Di Sotto A, and Villaño Valencia D
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- Body Composition, Diet, Fruit, Glucose, Humans, Students, Universities, Cardiovascular Diseases, Vegetables
- Abstract
Most university students do not follow recommendations for fruit and vegetable intake, with a consequent increase in the prevalence of cardiovascular disease (CVD) risk factors. The aim of this study was to compare obesity prevalence and biomarkers of metabolic status between Italian and Spanish university students, in relation with the consumption of fruits and vegetables. Food consumption, adherence to a Mediterranean diet (MD), level of physical activity (PA), blood glucose, total cholesterol, triglycerides and ketones, blood pressure, and body composition were evaluated. Among CVD risk factors, only glucose was significantly higher in Spaniards (SP), and only 3.1% of SP presented ketosis. SP had a higher percentage of energy from fat. Although adherence to MD and fruit and vegetable consumption did not differ between Italians and SP, students who consumed at least four servings of fruit and vegetables (FV group) showed better values for pressure and metabolic parameters than the no FV group. We observed an association between consumption of fruit and PA. Students who consumed more vegetables than fruit reported a better body composition profile and lower glucose concentrations. As previously suggested, in addition to PA, two servings of fruit and three servings of vegetables per day should be recommended.
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- 2022
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89. Total and Plant Protein Consumption: The Role of Inflammation and Risk of Non-Communicable Disease.
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Azzini E, Peluso I, Intorre F, Barnaba L, Venneria E, Foddai MS, Ciarapica D, Maiani F, Raguzzini A, and Polito A
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- Cross-Sectional Studies, Dietary Proteins, Female, Humans, Inflammation, Interleukin-15, Interleukin-6, Leptin, Male, Plant Proteins, Risk Factors, Tumor Necrosis Factor-alpha, Noncommunicable Diseases epidemiology
- Abstract
Background: Inflammatory cytokine levels are associated with Non-Communicable Diseases (NCDs) and can be influenced by a person’s macronutrient profile. This work aims to evaluate the relationship between the compliance with the age-specific recommended protein intake and the levels of inflammatory markers related to the risk of NCDs. Methods: The study participants included 347 participants (119 men and 228 women), ages 18 to 86 years. Cardio-metabolic risk evaluations, including an assessment of the prevalence of Metabolic Syndrome, were performed. Leptin, IL-15, IL-6, and TNF-α levels were measured. Results: The adequacy of the total protein (TP) intake was lower in old people compared to individuals aged <60 years, and only few volunteers consumed the suggested 50% plant protein (PP) for a healthy and sustainable diet. A lower risk of NCDs with a PP consumption above at least 40% was observed only in old individuals. A differential effect on TNF-α and IL-6 was observed for both TP and PP intake by gender and age class, whereas for leptin and IL-15 only significant interactions among sex and the class of age were found. Conclusion: Although our data suggest that consuming more than 40% of PP can reduce the risk of NCDs, the effect of gender differences on cytokine levels should be considered in larger studies.
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- 2022
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90. Polypoid arteriovenous malformation of the rectum: A case report.
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Krizzuk D, Cotesta M, Galiffa G, Peluso I, Falbo F, Biancucci A, Puscio S, Michelotto C, Pasecinic C, Montalto GM, and Sammartino F
- Abstract
Background: Intestinal arteriovenous malformation is an abnormal connection between arteries and veins that bypasses the capillary system and may be a cause of significant lower gastrointestinal bleeding. On endoscopy, arteriovenous malformations are usually flat or elevated, bright red lesions. Overall, rectal localization of arteriovenous malformations is rare. The same may be said about polypoid shape arteriovenous malformations. Herein, we present a case of a large rectal polypoid arteriovenous malformations., Methods: Clinical, diagnostic, and treatment modalities of the patient were reviewed. Pre- and post-operative parameters were collected and analyzed. The clinical English literature is also reviewed and discussed., Results: A 60-year-old female patient was admitted to our emergency department for rectorrhagia and anemia. Rectoscopy revealed a polypoid lesion in the rectum and the biopsy showed fibrosis, necrosis areas, and hyperplastic glands. A total body contrast-enhanced computed tomography (CT) was performed revealing a parietal pseudonodular thickening with concentric growth and contrast enhancement, extending for about 53 mm. The mass wasn't removed endoscopically due to concentric growth, sessile implant, and submucosal nature. The patient underwent an uneventful laparoscopic anterior rectal resection. The postoperative hospitalization was free of complications. Histology showed the presence of a polypoid AVM composed of dilated arteries, veins, capillaries, and lymphatics, engaging the submucosa, muscularis, and subserosa layer., Conclusion: After a review of the current English literature, we found only one case of rectal polypoid AVM. The scarcity of documented cases encumbers optimal diagnostic and treatment approaches., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (© 2022 Krizzuk, Cotesta, Galiffa, Peluso, Falbo, Biancucci, Puscio, Michelotto, Pasecinic, Montalto and Sammartino.)
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- 2022
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91. Genetic Analysis in Grain Legumes [ Vigna radiata (L.) Wilczek] for Yield Improvement and Identifying Heterotic Hybrids.
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Azam MG, Sarker U, Hossain MA, Iqbal MS, Islam MR, Hossain MF, Ercisli S, Kul R, Assouguem A, Al-Huqail AA, R H Mohamed H, and Peluso I
- Abstract
Six mungbean parental lines and their fifteen F
1 s produced from half-diallel mating design were investigated for combining ability and heterosis in terms of a yield and its components. Results showed highly significant variations among the parents and F1 s, suggesting a wide genetic variability for the studied characters. Analysis of variance indicated that genotypes mean square values, general combining ability (GCA) and specific combining ability (SCA) were highly significant ( p ≤ 0.001) for all measured traits except for days to flowering, days to maturity, and pod length indicating genetic diversity of parents and both additive and non-additive gene effects in the inheritance of the measured traits. A higher effect of SCA than GCA for plant height and seeds per pod suggests the preponderance of non-additive gene effects in the expression of characters. Based on per se performance and GCA, BARI Mung-1, PS-7, and BMXK1-14004 were the best general combiners for yield per plant. In the context of SCA, hybrids BMXK1-14004 × Sonali mung, BMXK1-14004 × PS-7, BMXK1-14004 × BINA Mung-8, Sukumar × PS-7, and BARI Mung-1 × BINA Mung-8 were good specific combiners. BMXK1-14004 × Sonali mung and BMXK1-14004 × PS-7 were the best heterotic hybrids for yield and yield-contributing traits. These parents and crosses could be utilized for further use in breeding programs to improve yields in mungbean crops.- Published
- 2022
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92. Synergistic Effect of Bioactive Monoterpenes against the Mosquito, Culex pipiens (Diptera: Culicidae).
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Ramzi A, El Ouali Lalami A, Annemer S, Ez Zoubi Y, Assouguem A, Almutairi MH, Kamel M, Peluso I, Ercisli S, and Farah A
- Subjects
- Animals, Eucalyptol pharmacology, Female, Larva, Monoterpenes pharmacology, Mosquito Vectors, Culex, Culicidae, Insecticides chemistry
- Abstract
Mosquitoes represent one of the most important vectors and are responsible for the transmission of many arboviruses that affect human and animal health. The chemical method using synthetic insecticides disturbs the environmental system and promotes the appearance of resistant insect species. Therefore, this study investigated the insecticidal effect of some binary monoterpene combinations (1,8 cineole + α-pinene and carvone + R (+)-pulegone) using a mixture design approach. The fumigant toxicity was evaluated against Culex pipiens female adults using glass jars. The results show that the toxicity varies according to the proportions of each compound. Indeed, Mixture 1 (1,8-cineole + α-pinene) displayed a strong toxic effect (51.00 ± 0.86% after 24 h and 100.00 ± 0.70% after 48 h) when the pure compounds were tested at 0.25/0.75 proportions of 1,8-cineole and α-pinene, respectively. Nevertheless, the equal proportion (0.5/0.5) of carvone and R (+)-pulegone in Mixture 2 exhibited a toxic effect of 54.35 ± 0.75% after 24 h and 89.96 ± 0.14% after 48 h, respectively. For Mixture 1, the maximum area of mortality that the proposed model indicated was obtained between 0/1 and 0.25/0.75, while the maximum area of mortality in the case of Mixture 2 was obtained between 0.25/0.75 and 0.75/0.25. Moreover, the maximum possible values of mortality that could be achieved by the validated model were found to be 51.44% (after 24 h) and 100.24% (after 48 h) for Mixture 1 and 54.67% (after 24 h) and 89.99% (after 48 h) for Mixture 2. It can be said that all purev molecules tested through the binary mixtures acted together, which enhanced the insecticide's effectiveness. These findings are very promising, as the chemical insecticide (deltamethrin) killed only 19.29 ± 0.01% and 34.05 ± 1.01% of the female adults after 24 h and 48 h, respectively. Thus, the findings of our research could help with the development of botanical insecticides that might contribute to management programs for controlling vectors of important diseases.
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- 2022
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93. Plant-Derived Smoke Solution Alleviates Cellular Oxidative Stress Caused by Arsenic and Mercury by Modulating the Cellular Antioxidative Defense System in Wheat.
- Author
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Ibrahim M, Nawaz S, Iqbal K, Rehman S, Ullah R, Nawaz G, Almeer R, Sayed AA, and Peluso I
- Abstract
Heavy metal stress is a significant factor in diminishing crop yield. Plant-derived smoke (PDS) has been used as a growth promoter and abiotic stress alleviator for the last two decades. Although the roles of PDS have been determined in various plants, its role in ameliorating heavy metal stress in wheat has not been reported so far. Therefore, the present work was conducted to investigate the effect of smoke solution extracted from a wild lemongrass Cymbopogon jwarancusa ( C. jwarncusa ) on physiological and biochemical features of wheat under arsenic (As) and mercury (Hg) stress. The results showed that higher concentrations of As and Hg pose inhibitory effects on wheat seed germination and seedling growth, including shoot/root length and shoot/root fresh weight. Photosynthetic pigments, such as chlorophyll a and b and carotenoids, were significantly decreased under As and Hg stress. Importantly, the levels of H
2 O2 , lipid peroxidation, and TBARS were increased in wheat seedlings. The activity of antioxidant enzymes, such as CAT, was decreased by As and Hg stress, while the levels of SOD, POD, and APX antioxidant enzymes were increased in root and shoot. Interestingly, the application of PDS (2000 ppm), individually or in combination with either As or Hg stress, enhanced wheat seed germination rate, shoot/root length, and shoot/root fresh weight. However, the levels of H2 O2 , lipid peroxidation, and TBARS were decreased. Similarly, the levels of SOD, POD, and APX were decreased by PDS under As and Hg stress, while the level of CAT was enhanced by PDS under As and Hg stress. Interestingly, the levels of chlorophyll a and b, and total carotenoids were increased with the application of PDS under As and Hg stress. It is concluded that PDS has the capability to alleviate the phytotoxic effects of As and Hg stress in wheat by modulating the antioxidative defense system and could be an economical solution to reduce the heavy metal stress in crops.- Published
- 2022
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94. Chemometric Investigation and Antimicrobial Activity of Salvia rosmarinus Spenn Essential Oils.
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Annemer S, Farah A, Stambouli H, Assouguem A, Almutairi MH, Sayed AA, Peluso I, Bouayoun T, Talaat Nouh NA, El Ouali Lalami A, and Ez Zoubi Y
- Subjects
- Anti-Bacterial Agents chemistry, Anti-Bacterial Agents pharmacology, Chemometrics, Eucalyptol, Gas Chromatography-Mass Spectrometry methods, Plant Oils chemistry, Oils, Volatile chemistry, Oils, Volatile pharmacology, Rosmarinus chemistry, Salvia
- Abstract
To ensure the better production and sustainable management of natural resources, a chemometric investigation was conducted to examine the effect of cooperative and harvesting periods on the crop yields and chemical compositions of Salvia rosmarinus Spenn essential oils in the Oriental region of Morocco. The samples were collected from three cooperatives over nine time periods from January 2018 to April 2019. The chemical composition of Salvia rosmarinus Spenn essential oils was analyzed by gas chromatography coupled with mass spectrometry. The data from this study were processed by multivariate analyses, including principal component analysis (PCA) and hierarchical cluster analysis (HCA). The disc diffusion technique and a determination of the minimal inhibitory concentration were performed to study the antibacterial properties of the oils. Statistical analysis showed that the cooperative and harvest period have a significant effect on yields. The highest yield of essential oil was recorded in April 2019 at cooperative C1. The PCA and the HCA results were divided into two groups: Group A for the summer season and group B for the winter season. The samples collected during summer were characterized by a high amount of 1,8-cineole component and a high yield of essential oil, whereas the samples collected during winter were qualified by a high amount of α-pinene component and a low yield of essential oil. The antibacterial activity of Salvia rosmarinus Spenn essential oils showed that Mycobacterium smegmatis ATCC23857 and Bacillus subtilis ATCC 23857 are the most susceptible strains, stopping growth at 1/500 ( v / v ). The least susceptible strain is Escherichia coli ATCC25922, with an MIC value corresponding to 1/250 ( v / v ). The findings of this study could have a positive economic impact on the exploitation of rosemary in the Oriental region, especially during the best harvest periods, as they indicate how to obtain the best yields of oils richest in 1,8-cineole and α-pinene chemotypes.
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- 2022
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95. Editorial: Therapeutic Index for Nutraceuticals in Inflammation-Related Diseases: Efficacy, Bioavailability, Metabolism and Interactions With Drugs, Volume II.
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Peluso I and Palmery M
- Abstract
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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- 2022
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96. The Limited Impact of Low-Volume Recreational Dance on Three-Compartment Body Composition and Apparent Bone Mineral Density in Young Girls.
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Milanese C, Cavedon V, Peluso I, Toti E, and Zancanaro C
- Abstract
Recreational dance is practiced worldwide as a multidimensional physical activity with a potential for prevention of a sedentary lifestyle and overweight/obesity. This study explored in young (7−15 year; n = 21) girls the effect of long-term (>1 year) exposure to recreational (2 h/w) dancing on three-compartment body composition. Recreational dancers (RD) were compared with recreational (≤4 h/w) artistic gymnasts (RG, n = 22) and physically active young girls not involved in structured extracurricular physical activity (control; C, n = 22), adjusting for confounding variables (age, body mass, menarche). We hypothesized for RD an intermediate body composition between RG and C. The three groups had similar age and body mass index. Body composition indices in RD were intermediate between that of C and RG, but RD values were not statistically significantly different vs. C. This agreed with the not statistically significant higher energy expenditure (MET-min/w) in RD vs. C (1357.7 ± 805.32 and 1090.9 ± 596.63, p = 0.172). In conclusion, long-term recreational dance exposure at low volume had limited positive effect on body composition of young girls vs. unstructured extracurricular physical activity. Future work will explore the potential of recreational dance at higher volume (3−4 h/w) to improve body composition in young girls.
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- 2022
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97. Alcohol Consumption by Italian and Spanish University Students in Relation to Adherence to the Mediterranean Diet and to the Food Neophobia: A Pilot Study.
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Aiello P, Peluso I, and Villaño Valencia D
- Abstract
This work aimed to relate alcohol consumption with adherence to the Mediterranean diet (MD) and with food neophobia (FN) among Italian and Spanish university students. Volunteers ( n = 194, 108 Italian and 86 Spanish), recruited at the La Sapienza University of Rome and the Catholic University of Murcia, filled in standardized questionnaires to evaluate alcohol consumption (AUDIT), FN (FN Scale: FNS), and adherence to the MD (MDS-14, MED-55, QueMD). In addition to the previously reported QueMD sub-score (aMED), a sub-score for non-typical MD foods (ntMED, carbonated and/or sugar-sweetened beverages (soft drinks), butter, margarine, or cooking cream, and manufactured sweets, pastries, and cakes) was evaluated. Italian females had higher MED-55 and FNS scores, and a lower AUDIT score than Spaniards ( p < 0.01). Students who stayed with their family (resident) were more adherent to MD than those who moved away from home. Resident Italians consumed less beer, hard liquors, and cocktails than Spaniards on Saturday nights ( p < 0.01). There were negative correlations between AUDIT and QueMD (R squared: 0.137, p < 0.05), and AUDIT and ntMED (R squared: 0.201, p < 0.01) in Spaniards, however, there was no relationship between AUDIT and other MD scores. In conclusion, this pilot study suggests that non-typical MD foods and Saturday night consumptions, related to being far from home, have a great impact on alcohol consumption.
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- 2022
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98. Editorial: Biomolecules Against Coronaviruses: Molecular Aspects, Multi-Omics and Systems Pharmacology.
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Hano C, Peluso I, and Chen JT
- Abstract
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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- 2022
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99. Hemorrhagic Cholecystitis. Report of a case with comprensive literature review and treatment algorithm.
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Krizzuk D, Peluso I, Pisani G, Falbo F, Montalto GM, Illuminati G, and Sammartino F
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- Humans, Hemorrhage etiology, Hemorrhage surgery, Cholecystectomy adverse effects, Abdominal Pain, Cholecystitis complications, Cholecystitis diagnosis, Cholecystitis, Acute surgery
- Abstract
Hemorrhagic Cholecystitis is a rare condition and usually represents a complication of acute cholecystitis. The clinical presentation is quite overlapping and usually involves abdominal pain that may be associated with fever, jaundice, nausea, vomiting, and finally haemobilia. It frequently involves patients with preexisting conditions such as chronic kidney disease undergoing hemodialysis or anticoagulation therapy. Due to the deadly potential of this condition attention must be high during diagnostics and treatment in order to avoid an ill-fated conclusion. To our knowledge, there is a lack of a comprehensive review on the subject as most of the literature consists of case reports or small case series. In order to give a contribution to improving the treatment strategy of this condition, we report a case successfully treated with cholecystectomy, and performed a literature review. Using the term "Hemorrhagic Cholecystitis", on PubMed database we found 67 cases reported in the English literature. The cases were analyzed by two researchers and clinical information was extrapolated and organized, aiming to create a comprehensive review on the subject, that may be clear and useful in clinical practice. KEY WORDS: Hemorrhagic cholecystitis, Surgical treatment.
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- 2022
100. Dietary Intakes and Food Habits of Wheelchair Basketball Athletes Compared to Gym Attendees and Individuals who do not Practice Sport Activity.
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Toti E, Cavedon V, Raguzzini A, Fedullo AL, Milanese C, Bernardi E, Bellito S, Bernardi M, Sciarra T, and Peluso I
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- Adult, Body Composition physiology, Diet, Mediterranean statistics & numerical data, Exercise physiology, Exercise statistics & numerical data, Humans, Italy epidemiology, Male, Sedentary Behavior, Spinal Cord Injuries epidemiology, Spinal Dysraphism epidemiology, Surveys and Questionnaires, Young Adult, Basketball statistics & numerical data, Eating physiology, Feeding Behavior physiology, Para-Athletes statistics & numerical data, Wheelchairs
- Abstract
Background: Mediterranean diet (Med-D) has been previously suggested for athletes, but Paralympics usually have a low intake of plant foods. Orthorexia nervosa (ON) can drive dietary intake of both athletes and gym attendees., Objective: We aimed to compare dietary intakes and food habits of elite wheelchair basketball athletes (WBA) and able-bodied individuals who practice or not sport activity and with different fat mass percentage (FM%)., Methods: We recruited 15 WBA from the Italian National team and 3 control groups (15 each group): healthy individuals who do not practice any sports activity (NSA) and gym attendees with low (GAL, FM%<17) and high (GAH, FM%>18) FM%. Food consumption was monitored by a 3- d diary, while Med-D scores and ON score were evaluated through standardized questionnaires. In WBA we also assessed Neurogenic Bowel Dysfunction (NBD), GastroEsophageal Reflux Disease (GERD), allergy questionnaire for athletes (AQUA) and Starvation Symptoms Inventory (SSI)., Results: In WBA, ON correlated with GERD and SSI. WBA and GAH with eating behavior of ON had higher adherence to Med-D, whereas NSA had less adherence to Med-D. Sub-score, including fruits, vegetables and legumes, was higher in the GAL and GAH groups compared to the WBA and NSA groups. Med-D was inversely related to animal protein intake (PRO-AN) in NSA and GAL. FM% was inversely related to PRO-AN in WBA and GAH, and to ON only in GAH. In WBA, PRO-AN and vegetable protein intake correlated with both carbohydrate and energy intakes., Conclusion: In WBA, commitment to wellness (ON and Med-D adherence) could be a response to gastrointestinal and starvation symptoms. WBA should be involved in setting their own individualized dietary strategies., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
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- 2022
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