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Functional analysis of regulatory elements controlling the expression of the ecdysone-regulated Drosophila ng-1 gene
- Source :
- Mechanisms of development, info:cnr-pdr/source/autori:Crispi S, Giordano E, D'Avino PP, Peluso I, Furia M./titolo:Functional analysis of regulatory elements controlling the expression of the ecdysone-regulated Drosophila ng-1 gene/doi:/rivista:Mechanisms of development (Print)/anno:2001/pagina_da:25/pagina_a:35/intervallo_pagine:25–35/volume:100
- Publication Year :
- 2001
- Publisher :
- Elsevier Scientific Ireland, Shannon , Paesi Bassi, 2001.
-
Abstract
- The steroid hormone ecdysone controls multiple aspects of insect development, including larval moults and metamorphosis, and can induce specific genetic responses in different tissues. The definition of the molecular mechanisms able to mediate this tissue-specific responsiveness may greatly contribute to understanding how such an accurate genetic response is achieved. In this work we have identified, by transgenic analysis, the regulatory elements directing the expression of ng-1, an ecdysone-regulated Drosophila gene showing a highly specific developmental expression profile. Our results show that an ecdysone-responsive element located within the ng-1 coding region is necessary for high-level gene expression, whereas the gene's spatial and temporal expression profile is fully controlled by a distinct upstream regulatory region. This region binds a set of transcriptional factors, including the FKH regulatory protein, which can potentially modulate the ecdysone genetic regulated response.
- Subjects :
- Ecdysone
Embryology
Time Factors
Transcription, Genetic
Transgene
media_common.quotation_subject
Molecular Sequence Data
Biology
Animals, Genetically Modified
chemistry.chemical_compound
Sequence Homology, Nucleic Acid
Gene expression
Animals
Drosophila Proteins
Coding region
Amino Acid Sequence
Salivary Proteins and Peptides
Metamorphosis
Gene
Transcription factor
media_common
Regulation of gene expression
Genetics
Base Sequence
Models, Genetic
Gene Expression Regulation, Developmental
Nuclear Proteins
Forkhead Transcription Factors
Blotting, Northern
beta-Galactosidase
Drosophila melanogaster
Lac Operon
chemistry
RNA
Transcription Factors
Developmental Biology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Mechanisms of development, info:cnr-pdr/source/autori:Crispi S, Giordano E, D'Avino PP, Peluso I, Furia M./titolo:Functional analysis of regulatory elements controlling the expression of the ecdysone-regulated Drosophila ng-1 gene/doi:/rivista:Mechanisms of development (Print)/anno:2001/pagina_da:25/pagina_a:35/intervallo_pagine:25–35/volume:100
- Accession number :
- edsair.doi.dedup.....67d571849559106cb700c57284cc0f6d