51. Decrease of GABA-immunoreactive neurons in the amygdala after electrical kindling in the rat
- Author
-
Kathryn A. Cunningham, Patricia Shinnick-Gallagher, Joseph M. Paris, and Patrick M. Callahan
- Subjects
Male ,medicine.medical_specialty ,3,3'-Diaminobenzidine ,In Vitro Techniques ,Amygdala ,Epileptogenesis ,Stereotaxic Techniques ,Epilepsy ,In vivo ,Internal medicine ,Kindling, Neurologic ,medicine ,Animals ,Electrodes ,Molecular Biology ,gamma-Aminobutyric Acid ,Neurons ,Chemistry ,Kindling ,General Neuroscience ,Rats, Inbred Strains ,medicine.disease ,Electric Stimulation ,Rats ,medicine.anatomical_structure ,Endocrinology ,nervous system ,Stereotaxic technique ,Immunohistochemistry ,Neurology (clinical) ,Neuroscience ,Nucleus ,Developmental Biology - Abstract
The present study was designed to investigate the effects of electrical kindling in vivo on GABA immunoreactivity (GABA-IR) of the lateral and basolateral amygdaloid nuclei 2-6 months post-stimulation. Male Sprague-Dawley rats were implanted with bipolar electrodes in the basolateral nucleus and stimulated once per day until 3-5 stage 5 seizures were observed. Coronal sections containing the amygdala were processed for GABA-IR using the contralateral side of the brain. Results indicate that, in comparison to controls, fully kindled animals showed a significant decrease in total number of GABA-IR amygdala neurons. Decreases in GABA-positive punctate structures surrounding unlabeled pyramidal cells were also observed, but not quantified. The present data suggest that epileptogenesis of the amygdala is associated with a significant reduction of GABA-IR in the lateral and basolateral areas throughout the contralateral amygdaloid nucleus.
- Published
- 1991