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367 results on '"Pas, Hendri H."'

55. Hyperkeratotic hand eczema: Eczema or not?

Author

Politiek, Klaziena, primary, Loman, Laura, additional, Pas, Hendri H., additional, Diercks, Gilles F.H., additional, Lemmink, Henny H., additional, Jan, Sabrina Z., additional, Akker, Peter C., additional, Bolling, Maria C., additional, and Schuttelaar, Marie L.A., additional

Published
2020
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57. Revertant mosaicism in epidermolysis bullosa caused by mitotic gene conversion

Author

Jonkman, Marcel F., Scheffer, Hans, Stulp, Rein, Pas, Hendri H., Nijenhuis, Miranda, Heeres, Klaas, Owaribe, Katsushi, Pulkkinen, Leena, and Uitto, Jouni

Subjects
Cell culture -- Usage, Immunofluorescence -- Usage, Electron microscopy -- Usage, Western immunoblotting -- Usage, Gene mutations -- Analysis, Keratinocytes -- Analysis, Polymerase chain reaction -- Usage, Mosaicism -- Research, Epidermolysis bullosa -- Analysis, Biological sciences
Abstract

Molecular biological techniques were used to investigate revertant mosaicism observed in a patient with generalized atrophic benign epidermolysis bullosa. The results confirmed that the clinical condition is caused by mitotic gene conversion of one of the two mutated COL17A1 alleles. Such reverse mutation was shown to be due to the nonreciprocal transfer of a part of one parental allele for the other.

Published
1997

64. The expression pattern of N‐acetyltransferase 1 in healthy human skin.

Author

Amerongen, Cynthia C. A., Kramer, Duco, Pas, Hendri H., and Schuttelaar, Marie L. A.

Subjects
HAIR dyeing & bleaching, BIOCONVERSION, AROMATIC amines, HUMAN beings, SAMPLE size (Statistics)
Abstract

Background: N‐acetyltransferase 1 (NAT1) is an enzyme expressed among others in keratinocytes in human skin. NAT1 is important in the biotransformation of aromatic amines, an important example being p‐phenylenediamine (PPD), a hair dye molecule. Unoxidized PPD penetrates the skin and is N‐acetylated by NAT1. Objectives: To investigate in detail the expression pattern of NAT1 in human skin. Materials and Methods: Cryosections obtained from healthy human skin were stained for NAT1 and expression patterns were observed. NAT1 double stainings were performed with antibodies against different cellular organelles to determine expression patterns. Result: A speckled, granular expression of NAT1 was seen predominantly in the stratum basale. NAT1 was expressed in a cytoplasmic pattern, perinuclear, and in the nucleus. No co‐localisation was seen with the selected cellular organelles. Local differences in NAT1 expression patterns were observed between donors and between different biopsies obtained from the same donor. Conclusions: NAT1 is expressed predominantly in the stratum basale and can be found in the cytoplasm, nucleus, and perinuclear in human skin. Further studies should be performed to investigate expression of NAT1 in a larger sample size. [ABSTRACT FROM AUTHOR]

Published
2021
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83. Particle Bombardment of Ex Vivo Skin to Deliver DNA and Express Proteins

Author

Sokol, Ena, Nijenhuis, Miranda, Sjollema, Klaas A, Jonkman, Marcel F, Pas, Hendri H, Giepmans, Ben N G, Clausen, Björn E., Laman, Jon D., Translational Immunology Groningen (TRIGR), Microbes in Health and Disease (MHD), Center for Liver, Digestive and Metabolic Diseases (CLDM), and ​Basic and Translational Research and Imaging Methodology Development in Groningen (BRIDGE)

Subjects
0301 basic medicine, Human skin, Transfection, Biology, Molecular biology, Green fluorescent protein, 030207 dermatology & venereal diseases, 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, 0302 clinical medicine, Plasmid, chemistry, Live cell imaging, Complementary DNA, Biophysics, Ex vivo, DNA
Abstract

Particle bombardment of gold microparticles coated with plasmids, which are accelerated to high velocity, is used for transfection of cells within tissue. Using this method, cDNA encoding proteins of interest introduced into ex vivo living human skin enables studying of proteins of interest in real time. Here, technical aspects of particle bombardment of ex vivo skin are described using green fluorescent protein (GFP) as readout for efficiency. This method can be applied on numerous tissues, including in living model animals.

Published
2017
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87. Whole-Genome Expression Profiling in Skin Reveals SYK As a Key Regulator of Inflammation in Experimental Epidermolysis Bullosa Acquisita

Author

Samavedam, Unni K., primary, Mitschker, Nina, additional, Kasprick, Anika, additional, Bieber, Katja, additional, Schmidt, Enno, additional, Laskay, Tamás, additional, Recke, Andreas, additional, Goletz, S., additional, Vidarsson, Gestur, additional, Schulze, Franziska S., additional, Armbrust, Mikko, additional, Schulze Dieckhoff, Katharina, additional, Pas, Hendri H., additional, Jonkman, Marcel F., additional, Kalies, Kathrin, additional, Zillikens, Detlef, additional, Gupta, Yask, additional, Ibrahim, Saleh M., additional, and Ludwig, Ralf J., additional

Published
2018
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88. Human retinal Müller cells synthesize collagens of the vitreous and vitreoretinal interface in vitro

Author

Ponsioen, Theodorus L., van Luyn, Marja J. A., van der Worp, Roelofje J., Pas, Hendri H., Hooymans, Johanna M. M., Los, Leonoor I., Restoring Organ Function by Means of Regenerative Medicine (REGENERATE), and Translational Immunology Groningen (TRIGR)

Subjects
EXPRESSION, EPIRETINAL MEMBRANE, Reverse Transcriptase Polymerase Chain Reaction, II PROCOLLAGEN, Blotting, Western, PROLIFERATION, In Vitro Techniques, DETACHMENT, Immunohistochemistry, AGE-RELATED-CHANGES, Retina, Culture Media, NEURAL RETINA, Vitreous Body, ASCORBIC-ACID, RABBIT, Humans, Tissue Distribution, sense organs, BODY, Collagen, RNA, Messenger, Cells, Cultured, Research Article
Abstract

Purpose: To investigate the capacity of cultured Muller cells to synthesize collagens, since previous studies indicated that Muller cells could be involved in collagen remodeling at the vitreoretinal border in adult human eyes. Methods: Spontaneously immortalized cultured human Muller cells were analyzed for the presence of mRNA of types I-VII, IX, XI, and XVII collagen by RT-PCR. Furthermore, Muller cells were immunocytochemically stained for light microscopic (LM) evaluation of these collagens and their main characteristics. Finally, cell extracts and culture medium were evaluated by western blot (WB) analysis using anticollagen antibodies. Results: Cultured Muller cells contained mRNA for types I-VII, IX, and XI collagen, but not for type XVII collagen. LM and WB confirmed the intracellular expression of all the above-mentioned collagens with the exception of type XVII. Collagen secretion into the medium was established for types I-VII, IX, and XI collagen. Conclusions: Cultured Muller cells can synthesize internal limiting lamina and vitreous collagens. Possible collagen production by Muller cells could explain and expand on previous in vivo morphological findings in the embryonic and postnatal period and in pathologic conditions.

Published
2008

89. Conditional depletion of mast cells has no impact on the severity of experimental epidermolysis bullosa acquisita

Author

Kasprick, Anika, Yu, Xinhua, Scholten, Julia, Hartmann, Karin, Pas, Hendri H., Zillikens, Detlef, Ludwig, Ralf J., Petersen, Frank, Microbes in Health and Disease (MHD), and Translational Immunology Groningen (TRIGR)

Subjects
conditional depletion, C-KIT EXPRESSION, autoimmunity, mast cells, KIT(W-SH/W-SH) MICE, epidermolysis bullosa acquisita, W-SH, DISEASE, Animal models, DEFICIENT W/WV, VII-COLLAGEN, EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS, PASSIVE TRANSFER, MUTANT MICE, IN-VIVO
Abstract

The role of mast cells (MCs) in autoimmunity is the matter of an intensive scientific debate. Based on observations in different MC-deficient mouse strains, MCs are considered as fundamental players in autoimmune diseases. However, most recent data suggest that the outcome of such diseases is strongly affected by the individual mouse strain used. By the use of two c-Kit mutant MC-deficient mouse strains and one c-Kit-independent strain, we here investigated the role of MCs in a systemic Ab transfer model of epidermolysis bullosa acquisita, a subepidermal autoimmune blistering skin disease characterized by autoantibodies against type VII collagen. While C57BL/6J-Kit(W-sh/W-sh) mice developed an unexpected increased blistering phenotype, no significant differences to WT controls were seen in WBB6F(1)-Kit(W/W-v) or the novel Mcpt5-Cre iDTR animals. Interestingly, in a local Ab transfer model, which induces a localized disease, we showed that application of high concentrations of anti-COL7 (where COL7 is type VII collagen) Abs induced MC activation and MC-dependent edema formation that did, however, not contribute to blister induction. Our results indicate that in the autoimmune disorder epidermolysis bullosa acquisita MCs do not contribute to the immune-mediated tissue injury. Modern c-Kit mutant-independent MC-deficient mouse strains will help to further redefine the role of MCs in autoimmunity.

Published
2015

90. Direct Immunofluorescence of Mechanobullous Epidermolysis Bullosa Acquisita, Porphyria Cutanea Tarda and Pseudoporphyria.

Author

DE GROOT, Heleen J., JONKMAN, Marcel F., PAS, Hendri H., and DIERCKS, Gilles F. H.

Subjects
EPIDERMOLYSIS bullosa, IMMUNOFLUORESCENCE, PSEUDOPORPHYRIA, SKIN diseases, FLUORESCENCE
Abstract

Mechanobullous epidermolysis bullosa acquisita (mEBA) can have a clinical presentation that is very similar to other blistering diseases, such as porphyria cutanea tarda (PCT) and pseudoporphyria. Direct immunofluorescence is an important feature in the diagnosis of mEBA, although features that overlap with PCT and pseudoporphyria have been reported. This retrospective observational study investigated whether direct immunofluorescence can discriminate mEBA from PCT and pseudoporphyria. Biopsies of 13 patients with mEBA, 10 with PCT and 10 with pseudoporphyria were included. In 7 cases of PCT and 4 of pseudoporphyria, direct immunofluorescence showed a pattern at the dermal-epidermal junction that appeared similar to the u-serrated pattern in mEBA. Vessel wall depositions were observed in all 3 diseases, but were more frequent and more intense in PCT and pseudoporphyria than in mEBA. Careful examination of direct immunofluorescence of mEBA vs. PCT and pseudoporphyria revealed different staining patterns, although overlapping features were present. Therefore, integrating all clinical and laboratory data is essential to differentiate between mEBA, PCT and pseudoporphyria. [ABSTRACT FROM AUTHOR]

Published
2019
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91. The smallest resonance energy transfer acceptor for tryptophan

Author

Broos, Jaap, Pas, Hendri H., and Robillard, George T.

Subjects
Tryptophan -- Spectra, Spectrum analysis -- Analysis, Energy transfer -- Analysis, Chemistry
Abstract

A study which reports about the utility of the diazirinyl group as acceptor in resonance energy transfer (RET) distance measurements with trytophan as donor is presented. The versatility of tryptophan (Trp) as an intrinsic spectroscopic probe in protein chemistry and the small size of the daizirinyl group makes a very attractive donor-acceptor couple for accurate RET distance information in protein chemistry.

Published
2002

93. PDE4 Inhibition as Potential Treatment of Epidermolysis Bullosa Acquisita

Author

Koga, Hiroshi, primary, Recke, Andreas, additional, Vidarsson, Gestur, additional, Pas, Hendri H., additional, Jonkman, Marcel F., additional, Hashimoto, Takashi, additional, Kasprick, Anika, additional, Ghorbanalipoor, Saeedeh, additional, Tenor, Hermann, additional, Zillikens, Detlef, additional, and Ludwig, Ralf J., additional

Published
2016
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96. Epiplakin Is a Paraneoplastic Pemphigus Autoantigen and Related to Bronchiolitis Obliterans in Japanese Patients

Author

Tsuchisaka, Atsunari, primary, Numata, Sanae, additional, Teye, Kwesi, additional, Natsuaki, Yohei, additional, Kawakami, Tamihiro, additional, Takeda, Yoshito, additional, Wang, Wenqing, additional, Ishikawa, Kazushi, additional, Goto, Mizuki, additional, Koga, Hiroshi, additional, Sogame, Ryosuke, additional, Ishii, Norito, additional, Takamori, Shinzo, additional, Hoshino, Tomoaki, additional, Brandt, Oliver, additional, Pas, Hendri H., additional, Fujiwara, Sakuhei, additional, and Hashimoto, Takashi, additional

Published
2016
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100. Autoantibodies to Multiple Epitopes on the Non-Collagenous-1 Domain of Type VII Collagen Induce Blisters

Author

Vorobyev, Artem, primary, Ujiie, Hideyuki, additional, Recke, Andreas, additional, Buijsrogge, Jacqueline J.A., additional, Jonkman, Marcel F., additional, Pas, Hendri H., additional, Iwata, Hiroaki, additional, Hashimoto, Takashi, additional, Kim, Soo-Chan, additional, Hoon Kim, Jong, additional, Groves, Richard, additional, Samavedam, Unni, additional, Gupta, Yask, additional, Schmidt, Enno, additional, Zillikens, Detlef, additional, Shimizu, Hiroshi, additional, and Ludwig, Ralf J., additional

Published
2015
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