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Particle Bombardment of Ex Vivo Skin to Deliver DNA and Express Proteins
- Source :
- Inflammation: Methods and Protocols, 1559(1), 107-118, Inflammation ISBN: 9781493967841
- Publication Year :
- 2017
-
Abstract
- Particle bombardment of gold microparticles coated with plasmids, which are accelerated to high velocity, is used for transfection of cells within tissue. Using this method, cDNA encoding proteins of interest introduced into ex vivo living human skin enables studying of proteins of interest in real time. Here, technical aspects of particle bombardment of ex vivo skin are described using green fluorescent protein (GFP) as readout for efficiency. This method can be applied on numerous tissues, including in living model animals.
- Subjects :
- 0301 basic medicine
Human skin
Transfection
Biology
Molecular biology
Green fluorescent protein
030207 dermatology & venereal diseases
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
0302 clinical medicine
Plasmid
chemistry
Live cell imaging
Complementary DNA
Biophysics
Ex vivo
DNA
Subjects
Details
- Language :
- English
- ISBN :
- 978-1-4939-6784-1
- ISBNs :
- 9781493967841
- Database :
- OpenAIRE
- Journal :
- Inflammation: Methods and Protocols, 1559(1), 107-118, Inflammation ISBN: 9781493967841
- Accession number :
- edsair.doi.dedup.....df1bfcb723d3aab2e0cd012c3840a3a3