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Composition, density and specimen sizes of pelagic polychaete assemblages were analyzed in the Southern Adriatic Sea. The study was based on finely stratified vertical (0–1100 m) and spatial sampling (17 stations) representing spring conditions. Holoplanktonic polychaetes were distributed in both neritic and pelagic waters, although the highest densities were observed along the Otranto Channel. Analysis of the size frequency distribution revealed a trend with depth only for some species. Spatial distribution of holoplanktonic polychaete density was not related to bottom depth, being the organisms mainly concentrated in the epipelagic layer (0–100 m). The most abundant species showed maximum values below or within the thermocline and within the Deep Chlorophyll Maximum or just above it. Relations between polychaete presence and the underlying oceanographic mechanisms regulating the circulation in the Otranto Channel were discussed. The presence of several non-determined polychaete larvae (e.g. Syllidae) in the pelagic waters at 800–1100 m depths suggests the importance of the role of Levantine waters as main actual and potential carrier of species in the area, though a relevant contribution comes also from North Adriatic dense waters through deep spilling and cascading in the Southern Adriatic pit. These findings increase the knowledge on holoplanktonic polychaetes ecology within the South Adriatic Sea, and represent significant data in the monitoring of changes in biodiversity.

Pollution deriving from textile wastes, including industrial and household waste, is recently of great interest due to their environmental impacts. Anthropogenic and synthetic fibers are responsible for negative effects on the quality of water and soil, and, also, their presence damages plant and animal health. In this work, the authors revealed the occurrence of man-made cellulose fibers in specimens of Boops boops from the Northern Sicilian coasts. Bogue was chosen as target species as it has been used as an indicator within the European Marine Strategy Framework Directive (MSFD 2008/56/EC) in order to value the “microplastics status” in the stomach contents. Of the 30 specimens examined, 63.3% of these had ingested fibers items. The number of fibers ranged from 1 to 10 per specimens with an average of 2.7 items/specimen. Fibers length ranged from 0.5 to 30 mm, most of them were black (95%), and a small percentage was red (5%). The ingestion of man-made cellulose fibers, observed for the first time, in Boops boops in the Mediterranean Sea wake-up call and it should attract the attention of the EU for new guidelines where this new type of contaminant is classified harmful as well as plastics.

Vanadium accumulation levels in different tissues (muscle and organs) of the striped mullet Mugil cephalus (Linnaeus, 1758) and possible relationships with blood parameters were evaluated in a Natural Protected Area (Lake Faro, Sicily, Italy), during the winter of 2017. Hematological parameters (red blood cell, RBC; white blood cell, WBC; hemoglobin concentration, Hb; hematocrit, Hct; mean corpuscular volume, MCV; mean corpuscular hemoglobin, MCH; mean corpuscular hemoglobin concentration, MCHC; thrombocytes, TC), biometric indices (weight, total and fork length), and vanadium levels in muscles and organs (gills, liver, stomach and intestine) were determined. Statistical analyses showed significant differences in concentrations of vanadium of the analyzed tissue of M. cephalus and a positive relationship between vanadium concentration in the liver and some hematological parameters (RBC, Hb and Hct) and biometric indices. Our results underline the importance of fish blood parameters as sensitive indicators of toxic impact of environmental factors such as metals. This study, focusing on an ongoing topic, represents a valuable contribution to research concerning the monitoring and prevention of vanadium pollution in aquatic organisms and environments.

Otoliths are used in taxonomy and ichthyology as they can provide a wide range of information about specimens. They are an essential tool to monitor the most sensitive species for a sustainable exploitation level. Despite the increasing use of sagittae in research, their inter- and intra-specific variability and eco-functionality are still poorly explored. This paper aims to investigate the inter- and intra-specific variability of Mugilidae sagittae using morphological and morphometrical analysis, as well as scanning electron microscopy and shape analysis. The sagittae of 74 specimens belonging to three different Mugilidae species, collected from a coastal lagoon, were analyzed to give an accurate description of their morphology, morphometry, shape and crystalline habits. The results highlighted the intra- and inter-specific variability of sagittae, showing morphometrical differences among species and slight differences between left and right sagittae in C. labrosus individuals. Moreover, SEM images showed a peculiar crystal organization, with several different crystal habits and polymorphs. This study provides an accurate description of sagittae in the studied species, deepening the knowledge on inter- and intra-specific variations and crystal habits and providing data which will be useful for future studies on otoliths. With this data, it will be possible to improve conservation and exploitation sustainability in sensitive habitats.

Microplastics (MPs) pollution is a threat to the marine environment worldwide (Andrady, 2011) from the tropics to the poles (Waller et al., 2017). The Mediterranean Sea, a semi-closed basin, is heavily affected by this pollution as well (Llorca et al., 2020). The aim of this special issue was to collect the latest studies on MPs pollution in the Mediterranean Sea. MPs are plastic particles smaller than 5 mm in size that derive from industrial products (primary microplastics) or from the fragmentation of large plastic pieces (secondary microplastics) (Auta et al., 2017). MPs are found floating on the surface, along with those in the water column, in sediments, and up to the deep sea (Van Cawenberghe et al., 2013). MPs can be ingested by aquatic biota and they can be a vehicle for other pollutants such as persistent organic pollutants (POPs), that can be adsorbed and concentrated from the surrounding seawater. As a result, MPs can be transfer several toxic pollutants via the food chain (Fytianos et al., 2020). This editorial introduces to the Research Topic “Microplastics in the Mediterranean Sea,” highlighting the pollution of MPs in this peculiar ecosystem and aiming at a better understanding of the occurrence, distribution, and toxicity presence and the study of MPs in the Mediterranean Sea.

Otolith features are useful tools for studying taxonomy, ecology, paleontology, and fish biology since they represent a permanent record of life history. Nevertheless, the functional morphology of otoliths remains an open research question that is useful to completely understand their eco-morphology. This study aims to deepen the knowledge of intra- and interspecific variation in sagitta morphology in three congeneric seabreams, to understand how such variability could be related to the lifestyles of each species. Therefore, the sagittae (n = 161) of 24 Pagellus bogaraveo, 24 Pagellus acarne, and 37 Pagellus erythrinus specimens, collected from the south Tyrrhenian Sea, were analyzed using scanning electron microscopy and a stereomicroscope to assess morphometric features, variability between otolith pairs and the external crystalline structure the of sulcus acusticus. Statistical analysis demonstrated that, between the species, variability in sagittal otolith rostral length growth and sulcus acusticus features, correlated with increased fish total length and body weight. Moreover, slight differences between otolith pairs were detected in P. acarne and P. erythrinus (P

The present investigation focuses on Boops boops specimens gathered in the Gulf of Patti in 2010. Providing a snapshot from the past, this paper represents, chronologically, the first record of microplastic ingestion in the Mediterranean bogue. The plastic abundance and composition in gastrointestinal tracts of the bogue was assess, in order to improve the knowledge on spatial-temporal variability of microplastics pollution in the Mediterranean basin and in particular, in the southern Tyrrhenian Sea. In a total of 65 specimens, 180 particles of plastic (2.8 items/specimens), mainly belonging to microplastics class, were found. Fragments (63%) and fibres (30%) were the predominant shape categories. Eleven polymers were identified: polypropylene and polyethylene were the most abundant. Several synthetic polymers belonging to the class of elastomers were also observed. The study area is strongly influenced by the absence of trawl fishing activities and a low mixing level of the seabed that, together with the confluence of different watercourses and the presence of different kind of anthropic impact, including motorway, could make it a 'waste disposal site'. Finally, our results suggest the usefulness to retrieve older samples to better understand spatial-temporal changes in marine litter pollution over time.

The aim of this study was to evaluate the daily total locomotor activity in captive Chrysocyon brachyurus. Three maned wolves were housed individually under natural light–dark cycle and environment...

Cetacean mechanical senses, such as hearing, echolocation, active touch and the perception of water movements, are essential for their survival. Dolphins skin possesses dense packing of dermal papillae associated with the cutaneous ridges that suggests a sensory function, furthermore they are well innervated and very sensitive to touch. This is mediated by mechanoreceptors, abundant in the region of the head and in the dorsal part of the body. Most odontocetes possess vibrissae (i.e., sensory hair) that have been well described in literature and present a microanatomy similar to that of terrestrial mammals. The aim of this study was to characterize Merkel cell through use of specific antibodies: Substance P, Anti-calbindin DK28, Anti-5HT, Leu- enkephalin, Protein Gene Product 9.5 (PGP9.5) and Anti-Human Neuronal Protein, for the first time. Merkel cells (MCs) in the dolphin skin are specialized skin receptors, characterized by their particular location and close association with nerve terminals. The presence of neuroendocrine markers and different neuropeptides confirms that MCs play also neuroendocrine function and are considered as part of the diffuse neuroendocrine system. Furthermore, the presence of Leu-enkephalin in Merkel cells could involve these cells in inflammatory responses in the skin.

Metal contamination is a serious environmental concern in the Middle East. Herein, geochemical fractionation distribution and potential sources of thirteen metals (Fe, Al, Mn, Zn, Cu, Co, Cr, Ni, V, As, Hg, Pb and Cd) were investigated in sediments from ten coral reef Islands in the Persian Gulf, Iran. To properly assess availability and mobility of elements, enrichment factor (EF), pollution load index (PLI), pollution index (PI), contamination index (CI), sediment pollution index (SPI) and ecological risk assessment were provided. Sediment grain size showed an outstanding role in controlling the levels of potentially toxic inorganic elements (PTIEs). The highest values of total organic matter (TOM) were detected in Kharg and Lavan Islands. Different metals fractionation distribution was found across sites. As was noticed in carbonate (F2), exchangeable (F1), Fe-Mn oxy-hydroxide (F3), organic (F4) and residual (F5) fractions, Hg primarily associated with F2 and F1, whereas Pb and Cd with F2, followed by F1, F3, F5 and F4. Conversely, Ni and V accumulated in F1, suggesting their high mobility and bioavailability, and thus environmental risk to aquatic biota. All metals (except Al, Fe and As) had geological and anthropogenic sources. Based on modified risk assessment analysis, the sediments from Kharg, Lavan, Siri and Lark Islands showed medium adverse effects. Overall, results from this study corroborate that petroleum industry is the main source of pollution of PTIEs in the Persian Gulf, and offer a scientific basis for monitoring and preventing metal pollution in the environment.

We reviewed literature on the diet of the Galeus melastomus Rafinesque, 1810, from the Mediterranean Sea. Specific keywords (“Galeus melastomus diet”, “feeding habits”, “trophic position”, “biology”, “deep environment adaptation”) in the principal data sources, such as Web of Science, PubMed, and Google Scholar were used. Seventeen studies conducted on the diet and trophic position of G. melastomus have been considered for Mediterranean Sea regions. The feeding habits have been analyzed in many areas of the western basin; instead, for the Tyrrhenian, Adriatic, and central Mediterranean Seas, information is outdated and fragmentary. In all investigated sub areas, the data showed that G. melastomus is an opportunistic demersal supra benthic predator, benthic feeder, and scavenger, that adapts its diet to the seasonal and geographical fluctuations of the prey availability. It occupies a generalist niche showing individual specialization. In all reviewed Mediterranean sub areas, the most important prey groups were crustaceans, cephalopods, and teleost fishes. Taxa percentage in its diet composition can vary depending on different habitats with ontogenetic development of individuals, depth (that is correlated with the ontogenetic development), seasonal availability, and distribution of different prey groups. Widening knowledge of G. melastomus feeding habits is a fundamental tool for better understand meso and bathy-pelagic ecosystems.

Data on the biology and ecology of Galeus melastomus are old/absent for the Southern Tyrrhenian Sea, despite there being numerous studies in the wider area. A total of 127 specimens of G. melastomus from the southern Tyrrhenian Sea, collected in 2018–2019 using trawling nets, were analyzed to investigate size at sexual maturity, sex ratio, length–weight relationships, and feeding habits. To our best knowledge, this is the first time in which all these features were investigated in the Southern Tyrrhenian Sea for G. melastomus. The stomach content analysis showed that G. melastomus had intermediate feeding habits, preying on a great variety of species, especially Cephalopoda, Osteichthyes, and Crustacea. The Levin’s index value (Bi) was 0.53. Sex ratio was 0.92:1, with females slightly more abundant and bigger than males. The results also showed a decrease (33.7 cm for females, 31.1 cm for males) in length at 50% maturity (L50). This could be a result of anthropogenic stressors, such as overfishing and/or and environmental changes, which can induce physiological responses in several species. Our results highlighted the differences related to sexual maturity, growth, and feeding habits of the blackmouth catshark in the studied area, providing reference data to allow comparison with future studies on this species adaptations to this and other deep-sea areas in the Mediterranean Sea.

Microplastics (MPs) are widespread in the aquatic environmental due to anthropogenic pollution and are ingested indifferently by fishes that confuse them with food. In aquatic environment, mechanical stress, UV radiations, chemical and biological actions cause a constant degradation and breakdown of plastic objects into smaller fragments. Mediterranean Sea is characterized by the highest densities of plastics in the world being a closed basin with a complex hydrodynamics. The aim of this work is to evidence the occurrence of microbial adhesions on MPs found in edible Pagellus erythrinus (Linnaeus, 1758) bought in local Sicilian supermarkets and therefore highlight the potential role of microplastics in conveying antibiotic resistance by ingestion of food by humans. The composition and structural-morphological properties of MPs found in the excised gastrointestinal tract (GIT) and gills are identified using different techniques. In particular, microparticles of different nature (plastics, organic components, cellulose-based materials) have been determined following specific Raman signals on a large spectral range (300-3500 cm-1). The Pagellus was dissected, open longitudinally, within a sterile glass Petri dish. The components deemed exogenous to the normal structure of the gills and GIT were collected. Optical microscopy images showed that both the GIT and the gill of Pagellus erythrinus contain MPs of different colors (black, dark blu) and mainly with a fibrous shape. At the moment, microbial assays show the adhesion of Citrobacter and E. coli in some fibers extracted by gills while Vibrio spp was mainly detected in the fibers present in GIT. Bacterial isolates were screened for susceptibility to antibiotics using the Kirby-Bauer test, choosing the molecules most used in human therapy. The results obtained suggest that plastics may contribute to the spread of multiple antibiotic resistance in marine environments underline the relevance of future studies on this topic. Key messages Plastics can serve as vectors for the spread of multiple resistances to antibiotics across marine environments. Further studies on possible vehicles of multidrug-resistant germs carried by food of various kinds are desirable.

The Strait of Messina (Sicily, Italy) attracts the interest of marine ecologists for the presence of a large variety of habitat and mutually-interacting communities. Among them, beachrock formations, despite their wide geographic distribution, which also includes the Mediterranean area, have been poorly investigated from the biotic viewpoint. In this paper, the spatial and seasonal variability of benthic megafauna from the Messina microtidal beachrock is described. Combining in situ collected data (measurements of abiotic parameters and underwater visual census) with theoretical post-processing analyses (analysis of similarity percentages and cluster analysis), we deduced the possibility to model the dynamics observed between the most dominant species, a top snail, Phorcus turbinatus (Born, 1778), and a hermit crab, Clibanarius erythropus (Latreille, 1818), in terms of a prey-predator interaction. These species gave rise to different intriguing dynamical regimes (including periodic oscillations) that were qualitatively captured by a mathematical model focused on the respective trophic chain levels. The identification of all model parameters and the use of numerical simulations complemented the above analysis and allowed to gain more insights into the complex dynamics of these oligotypic communities and on the most relevant factors determining the ecosystem equilibria.

This study reports the returning of the seahorse in the Faro Lake and the most recent finding in a brackish coastal Mediterranean lagoon. After about 50 years from the first documented occurrence o...

This study highlights plastics occurrence in five demersal fish species from the Southern Tyrrhenian Sea: the Red mullet Mullus barbatus barbatus, the Piper gurnard Trigla lyra, the Blackmouth catshark Galeus melastomus, the Lesser spotted dogfish Scyliorhinus canicula and the Brown ray Raja miraletus. Overall, 125 fish were examined: 21 Red mullets, 16 Piper gurnards, 75 Blackmouth catsharks, 72 Dogfish and 1 Brown ray. The percentage of fish with ingested plastics was 14.4% with 0.24 items per specimen. The majority of the debris were fibers and the application of infrared and Raman spectroscopy allowed the identification and discrimination of plastic and non-plastic fibers. The plastic debris isolated were mainly microplastics (94.1%), while macroplastics occurrence was very low (5.9%). The plastics were identified as polypropylene, Teflon, nylon, kraton G (triblock copolymer) and polyethylene. Also cellulose was detected. S. canicula was the species with the highest number of plastic pollutants.

The phylum Cnidaria is one of the most important contributors in providing abundance of bio- and chemodiversity. In this study, a comprehensive chemical investigation on the nutritional and nutraceutical properties of Mediterranean jellyfish Pelagia noctiluca was carried out. Also, compositional differences between male and female organisms, as well as between their main anatomical parts, namely bell and oral arms, were explored in an attempt to select the best potential sources of nutrients and/or nutraceuticals from jellyfish. With the exception of higher energy densities and total phenolic contents observed in females than males, no statistically significant differences related to the specimen&rsquo, s sex were highlighted for the other compound classes. Rather, the distribution of the investigated chemical classes varied depending on the jellyfish&rsquo, s body parts. In fact, crude proteins were more abundant in oral arms than bells, saturated fatty acids were more concentrated in bells than oral arms, whereas polyunsaturated fatty acids were distributed in the exact opposite way. On the other hand, major elements and trace elements demonstrated an opposite behavior, being the latter most accumulated in oral arms than bells. Additionally, important nutraceuticals, such as eicosapentaenoic and docosahexaenoic acids, and antioxidant minerals, were determined. Overall, obtained data suggest the potential employment of the Mediterranean P. noctiluca for the development of natural aquafeed and food supplements.

The pen shell Pinna nobilis (Linnaeus, 1758) is the largest endemic bivalve mollusc of the Mediterranean Sea, listed as an endangered species in the European Union. Because no information is available about the adaptation of pen shells to different habitats, herein the fundamental conditions of adaptation of P. nobilis to peculiar natural environments, such as the Strait of Messina (SM) and the meromictic Faro Lake (FL; Sicily, Italy), were explored by assessing the morphology, mucous production, osmoregulation and neurotransmission of their gills. Although gills of the pen shells from both sites exhibited a regular morphology, a higher presence of acid mucous cells was detected in P. nobilis from FL than SM, as well as higher levels of osmolytes but without interfering the osmoregulatory processes. About the functioning of gills, the cholinergic (i.e. acetylcholine and AChE) neuronal system was unaltered between individuals from the two sites, whereas the GABAergic neurotransmission (i.e. 4-aminobutyrate or GABA) was significantly augmented in gills of P. nobilis from FL than SM. This may be an adaptive response to hypoxic conditions in FL, as supported by the increased hypoxia-inducible factor (HIF-1α) in gills of pen shells from FL than SM. Noteworthy, this study reports for the first time the presence of the GABA neurotransmitter within the metabolite profile, obtained by application of a protonic Nuclear Magnetic Resonance (1H NMR)-based metabolomics approach, of a marine bivalve. Therefore, GABA may be suggested as a metabolite biomarker in pen shells. Overall, findings from this study provide new insights on the behavioural and adaptive responses of the pen shell Pinna nobilis settled in different peculiar environments.

The present study investigates the occurrence of plastic pollution in two commercially important marine teleosts ( Zeus faber and Lepidopus caudatus ) from the northern coasts of Sicily (Tyrrhenian Sea). Plastics occurrence in the gastrointestinal tract was higher in Lepidopus caudatus (78.1%) than Zeus faber (51.4%). Debris characterization, carried out by micro-Raman spectroscopy, allowed identified the main types of found polymers as: polypropylene (PP), polyamide (PA), nylon and, to a lesser extent, polyethylene (PE). Of the two fish species studied, the silver scabbardfish appeared to be the more vulnerable to plastic ingestion. Our study represents a starting point that may pave the way for future investigation of the fate, accumulation and transfer of plastic debris to upper trophic levels, to verify their potential toxicity and to better understand strategies to mitigate this phenomenon.

Sponges represent important habitats for a community of associated (micro)organisms. Even if sponges dominate vast areas of the Antarctic shelves, few investigations have been performed on Antarctic sponge-associated bacteria. Using a culture-dependent approach, the composition of the bacterial communities associated with 14 Antarctic sponge species from different sites within the Terra Nova Bay (Ross Sea) area was analyzed. Overall, isolates were mainly affiliated to Gammaproteobacteria, followed by Actinobacteria and CF group of Bacteroidetes, being the genera Pseudoalteromonas, Arthrobacter and Gillisia predominant, respectively. Alphaproteobacteria and Firmicutes were less represented. Cluster analyses highlighted similarities/differences among the sponge-associated bacterial communities, also in relation to the sampling site. The gammaproteobacterial Pseudoalteromonas sp. SER45, Psychrobacter sp. SER48, and Shewanella sp. SER50, and the actinobacterial Arthrobacter sp. SER44 phylotypes occurred in association with almost all the analyzed sponge species. However, except for SER50, these phylotypes were retrieved also in seawater, indicating that they may be transient within the sponge body. The differences encountered within the bacterial communities may depend on the different sites of origin, highlighting the importance of the habitat in structuring the composition of the associated bacterial assemblages. Our data support the hypothesis of specific ecological interactions between bacteria and Porifera.

In this work it is reported for the first time the characterization of microplastics from sea water samples and in two congener species of seabreams: Pagellus erythrinus and P. bogaraveo, Mediterranean fish species of great commercial importance. An experimental survey was conducted on May-June 2017 in the southernmost part of the Tyrrhenian Sea. Microplastics found in the sea water and in the grastrointestinal tract of two teleosts were characterized by Raman and IR spectroscopies. Microplastics found in sea water samples appeared in the form of fragments made of plastics of low and high density (PVC and LPDE). All the microplastics found in fish belonged to Nylon 66, typical fibers used in industry and in fisheries. Our findings highlighted the importance of further studies along the food web chain for a better understanding of the diffusion and possible consequences of this terrible threat.

In the present study, it has been evaluated how 10 µm of polyethylene microspheres can be ingested by Artemia salina (Linnaeus, 1758) larvae within the first 7 days of the life cycle, and the impact on their health. Twelve A. salina larvae (instar I) groups were exposed to different microplastics (MPs) concentrations (0-1-10-102-103-104 MPs/mL), with and without Dunaliella salina as a food source. The results highlighted that A. salina larvae ingest MPs in relation to the exposure times in a dose-dependent manner and are significantly influenced by food availability. The highest contamination found was 306.2 MPs/individual at 104 MPs/mL exposure without a food source. No MPs were found in the presence of the food source from 1 to 102 MPs/mL, while contamination was detected at all concentrations of MPs without a food source. The worst effect on the developmental stages was evaluated at 168 h with a food source, with a delay compared to the control of I and II instars at 103 and 104 MPs/mL, respectively. Furthermore, microalgal feeding was significantly reduced for about 50% in the presence of 104 MPs/mL. These results highlight that aquatic microplastics pollution could affect the A. salina’s feeding behavior and life cycle.

Trabajo presentado en el 11º Congreso Ibérico, 8º Iberoamericano de Contaminación y Toxicología (CICTA 2018) celebrado en la Escuela Técnica Superior de Ingeniería de Montes, Forestal y del Medio Natural, Madrid del 11 al 13 de julio de 2018, There is a rising interest for both the conservation and biotechnological application of Sea anemones belonging to Anthozoa Class, (Cnidaria Phylum). Global warming has been recognised as one of the most important hazards for some species but the risk posed by pharmaceuticals and other emergent compounds present in the coastal waters they inhabited have been less studied. Anemonia sulcata and Actinia equina are widely distributed species in the Mediterranean Sea. The objective of this work has been to determine the physiological and biochemical effects of carbamazepine (CBZ) exposure as well as to characterize the degradation routes followed by this compound in water and tissues (biotransformation) of the two anemones species. Anemones provided by Oceanografic of Valencia Aquarium were acclimatized to laboratory conditions for two weeks and exposed to 1 ¿g/L and 100 ¿g/L of CBZ in artificial sea water in a semi-static system. Small portion of the tentacles were taken after two and seven days. Ion transport (measured as NKATPase activity), energetic metabolism (measured as glucose and lactate levels) and nitrogen excretion (measured as ammonia concentration in tissues) were determined. Treated samples of A. sulcata and A. equina were analysed by ultra-high-performance liquid chromatographyhigh-resolution mass spectrometry on a quadrupole-time-of-flight (QqTOF) (TripleTOF®, ABsciex). The structures of metabolites have been proposed using HRMS and HRMS/MS data. In particular, the combination of mass accuracy and the fragmentation patterns of metabolites and parent compound allowed proposing plausible structures for each metabolite. CBZ metabolites originated from aromatic and benzylic hydroxylation and epoxidation. The analysis of the water samples saved from each aquarium pointed out that a small percentage of CBZ can be degraded by hydrolysis and/or oxidation to the hydroxylated metabolites but not to the epoxidized ones. The current work constitutes the first study on the identification of Cnidaria metabolites of CBZ in species of the Anthozoa Class.

Among filter-feeders, pennatulids are the most complex and polymorphic members of the cnidarian class Anthozoa. They display a wide distribution throughout all the oceans, constituting a significant component of the sessile megafauna from intertidal to abyssal depths. In this study, a total of 118 bacterial isolates from enrichment cultures, carried out with homogenates of the sea pen Pteroeides spinosum (Ellis, 1764), were screened for hydrocarbon utilization by using the 2,6-dichlorophenol indophenol assay. Among them, 83 hydrocarbon-oxidizing isolates were analyzed for biosurfactant production by standard screening tests (i.e., emulsifying activity, E24 detection, surface tension measurement, microplate assay). The 16S rRNA gene sequencing revealed the affiliation of the most promising isolates to the genera Brevibacterium and Vibrio. Biosurfactant production resulted strongly affected by salinity and temperature conditions, and occurred in the presence of diesel oil and/or crude oil, whereas no production was observed when isolates were grown on tetradecane. The strains resulted able to create stable emulsions, thus suggesting the production of biosurfactants. Further analyses revealed a glycolipidic nature of the biosurfactant extracted from Vibrio sp. PBN295, a genus that has been only recently reported as biosurfactant producer. Results suggest that pennatulids could represent a novel source for the isolation of hydrocarbon-oxidizing bacteria with potential in biosurfactant production.

We report the presence of microplastics on the external surface and in the gastrointestinal tract of white late-larval and juvenile stages (fry) of clupeid fishes caught in the Southern Tyrrhenian Sea. The average highest number of plastics debris was recorded on Sardina pilchardus (0.53 items/specimen); a lower average number of items was observed for Engraulis encrasicolus (0.26 items/specimen). The plastics were characterized by fibers that differed in shape, colour and composition. Polyester, polypropylene, polyacrylonitrile, polyethylene, polyamide, nylon, rayon and polyurethane segments were detected by Raman and FTIR spectroscopies. Traces of organic components and dyes, compounds that are generally included in the polymer matrix to modify its base properties, were also identified on microplastics. Our results raise concerns for the potential transfer of synthetic materials through the marine food web and into humans, given the prominent role of S. pilchardus and E. encrasicolus within the food web as main food source for many marine species.

Changes in the composition and biomass distribution of deep-living zooplankton over wide gradients of depth (0–1300 m) have been analyzed in the Central Ligurian Basin (Northwestern Mediterranean Sea), seeking the environmental variables responsible for these changes. Spring vertical distribution (early May 2013) and diel vertical migration (DVM) of some key species of macroplankton and micronekton communities were studied every 6 h during a 24 h cycle. The hauls, performed with the multiple opening/closing 230 µm BIONESS net system, made possible a detailed description of the vertical distribution of twenty-three species: one siphonophore, one pyrosomid, one salp, three pteropods, four amphipods, one mysid, eight euphausiids, two decapods, two fishes. Cluster analysis performed on major species of macroplankton and micronekton community assemblage mainly highlighted the presence of four well defined assemblages, based on the depth range in the water column: 0–60 m, 60–100 m, 100–600 m and from 600 to 1300 m. During daytime, vertical distributions of biomass exhibited similar profiles, with maxima in the 0–60 m layer. Highest abundances were recorded between 20–40 and 40–60 m depth, at midnight and in the morning. The highest values of both biodiversity and number of species were found in a deeper layer comprised between 400 and 600 m. Most of the species showed similar distributions throughout the sampled area, either with typical non-diel-migrating characters such as the euphausiid Stylocheiron longicorne and the fishes Cyclothone braueri and C. pygmaea, or with clear diel-migrant behaviour (e.g. the siphonophore Chelophyes appendiculata and the euphausiid Euphausia krohni). Some species (e.g. Vibilia armata, Phronima sedentaria, Scina crassicornis, Salpa fusiformis, Cavolinia inflexa, Gennadas elegans) exhibited a bimodal vertical distribution that could be attributed to different causes able to influence and modify their vertical migration. The lower values of chlorophylla concentrations found in the present studyinthe month of May corresponds to the transition period from a mesotrophic system to a rather oligotrophic one. Temperature and chlorophyll were the variables that principally influenced the distinction between shallow (0–60 m) and deep communities. Below 60 m depth, the dissolved oxygen and salinity become the most important variables affecting the zooplankton community.

The air-breathing specialization has evolved idependently in vertebrates, as many different organs can perfom gas exchange. The largest obligate air-breathing fish from South America Arapaima gigas breathe air using its gas bladder, and its dependence on air breathing increases during its growth. During its development, gill morphology shows a dramatic change, remodeling with a gradual reduction of gill lamellae during the transition from water breathing to air breathing . It has been suggested that in this species the gills remain the main site of O2 and CO2 sensing. Consistent with this, we demonstrate for the first time the occurrence of the neuroepithelial cells (NECs) in the glottis, and in the gill filament epithelia and their distal halves. These cells contain a broader spectrum of neurotransmitters (5-HT, acetylcholine, nNOS), G-protein subunits and the muscarininic receptors that are coupled to G proteins (G-protein coupled receptors). We report also for the first time the presence of G alpha proteins coupled with muscarinic receptors on the NECs, that are thought as receptors that initiate the cardiorespiratory reflexes in aquatic vertebrates. Based on the specific orientation in the epithelia and their closest vicinity to efferent vasculatures, the gill and glottal NECs of A. gigas could be regarded as potential O2 and CO2 sensing receptors. However, future studies are needed to ascertain the neurophysiological characterization of these cells.

This study reports the returning of the seahorse in the Faro Lake and the most recent finding in a brackish coastal Mediterranean lagoon. After about 50 years from the first documented occurrence of

In the present study zebrafish (Danio rerio) has been used as model organism to establish the effects of dietary supplementation of Gracilaria gracilis powder (GP) on mucosal and innate immune parameters, antioxidant enzymes, and growth. In order to establish these features, zebrafish were fed for eight weeks with experimental diets containing different levels of Red algae, 0.25, 0.5 and 1% of GP; also, a group was fed with control diet. At the end of the experimental period the antioxidant superoxide dismutase and catalase (SOD, CAT) genes expression, interleukin 1 beta (il-1β), lysozyme (LYZ), tumor necrosis factor alpha (TNF-α) for immune-related genes expression, total immunoglobulin (Ig), total protein, alkaline phosphatase (ALP) activity for innate immune parameters, and growth performance have been established. The GP dietary supplementation showed differences in SOD and CAT expression in zebrafish whole body respect to the control group. Non-signifcant differences were noticed among the different groups in case of TNF-α, LYZ and il-1expression (P 0.05). The skin mucus total Ig and total protein in the group fed on 1% of GP were significantly higher respect to control group (P 0.05). 0.25 and 0.5% of GP dietary supplementation significantly enhanced skin mucus ALP activity levels (P 0.05). No significant differences were recorded for growth performances among groups (P 0.05). The results obtained in the present study revealed that G. gracilis could be takes in account as fishes diet supplementation for its immune system stimulants effects.

This study was aimed at gaining insights on the prokaryotic community (in terms of both taxonomic composition and activities) inhabiting the active layer at Edmonson Point, an ice-free area on the eastern slope at the foot of Mount Melbourne (Northern Victoria Land, Antarctica). Samples were collected during the thawing period, when microbial physiological activities are restored to utilize previously frozen organic substrates. Despite the very small cell sizes (600 daltons) substrates, as indicated also by the obtained rates of enzymatic hydrolytic activities over proteolytic, glycolitic and phosphoric compounds. Taxonomical composition showed that Proteobacteria, Actinobacteria and Firmicutes dominated the prokaryotic community, with most of their members playing crucial roles in organic matter turnover, as well as nitrogen cycling, or entering a viable but not cultivable state to cope with continuously changing environmental conditions, such as in the case of the active layer. Finally, non-autochthonous bacteria (mainly of marine origin) were detected and they probably contribute to the organic matter turnover within such cold terrestrial habitat. This research provides the first comprehensive account of the prokaryotic communities inhabiting the Antarctic permafrost and contributes to existing information on the response of their abundance and metabolism in a permafrost area that undergoes to seasonal changes (e.g. in terms of temperature, water availability and ice presence).

Enormous marine biodiversity offers an endless reservoir of chemicals for many applications. In this scenario, the extraction of seaweeds represents an interesting source of compounds displaying antimicrobial activity. In particular, among the different red algae, Gracilaria gracilis plays an important role due to the presence of important bioactives in its composition. In spite of these features, an efficient culture system is still absent. In the present study, a novel algal culture method was developed and compared to another more common cultural practice, widely reported in literature. A higher efficiency of the new method, both for daily growth rate and biomass, was assessed. Furthermore, the growth inhibitory activity of five extracts, obtained using ethanol, methanol, acetone, chloroform or diethyl ether as a solvent, from the cultured G. gracilis was tested against Gram-positive and Gram-negative pathogens. Algal extracts exhibited a considerable inhibitory activity against B. subtilis strains, while a slight inhibition was observed against V. fischeri. The different extracts showed significant differences in bacterial growth inhibition, with the highest activity that was recorded for the ethanol extract, followed by that of methanol. Based on the chemical characterization, these findings could be related to the antimicrobial activity played by the combination of total carbohydrates and polyphenols, which were determined at high levels in ethanol and methanol extracts, as well as by the highest number and levels of single polyphenols. Conversely, the lower growth inhibitory activities found in chloroform and diethyl ether extracts could be related to the isolation of minor lipid classes (e.g., neutral and medium polar lipids) composed by fatty acids, such as stearic, oleic and arachidonic acids, typically characterized by antimicrobial activity. In consideration of the results obtained, the present study has a double implication, involving both the field of cultural practices and the exploitation of natural sources for the isolation of antimicrobial agents useful both in pharmaceutical and food applications.

Physical disturbance by trawling can have both negative and positive effects on populations of scavenging benthic organisms. In the present study the impact of fishing activity on feeding behaviour of the two Astropectinids, Astropecten bispinosus and A. irregularis , was assessed based on stomach contents analysis. The study was carried out along trawled seabed highlighting the positive response of the two facultative scavengers to carrion generated by trawl disturbance. Furthermore, there was greater food specialization in areas that were more heavily exploited by trawling. This specialisation could be linked to the availability of certain prey that results from the passage of fishing gears across the seabed. Interestingly, differences between the two species analysed have been highlighted in term of population dynamic, feeding rate, diet composition and diet diversity, testifying their capacity to coexist in the same fishing grounds. Normal 0 false false false EL X-NONE X-NONE

The branchiuran parasite Argulus vittatus (Crustacea: Branchiura: Argulidae) was collected from the pectoral fin of the axillary bream Pagellus acarne, comber Serranus cabrilla, and greater amberjack Seriola dumerili from the northern coasts of Sicily (Mediterranean Sea), Italy. Pagellus acarne, Serranus cabrilla, and Seriola dumerili are new hosts for A. vittatus.

The ability to observe and predict trawling-induced patterns at spatial and temporal scales that are relevant to inform realistic management strategies is a challenge which scientists have consistently faced in recent decades. Here, we use fish feeding behaviour, a biological trait easily impaired by trawling disturbance, to depict alterations in fish condition (i.e. individual fitness) and feeding opportunities. The benthivorous fish Mullus barbatus barbatus was selected as a model species. The observed trends of responses to trawling in prey species confirmed the effectiveness of a non-trawled zone in sustaining higher levels of diet diversity (e.g. quantity and quality of ingested prey) and fish condition values (e.g. morphometric and physiological Condition Index). Changes observed in fish prey selection confirmed the role of trawling disturbance in modifying the local soft bottoms community, producing alterations of prey availability that trigger shifts in fish diet. Trawling-induced feeding patterns, mirrored through stomach contents, can positively or negatively affect fish condition, the main driver of population dynamics in maintaining carrying capacity levels. Due to the widespread socio-economic value of the red mullet fishery, and the current exploitation status, evidence gathered by the proposed bottom-up trait based approach might inform future trawling adaptation strategies, and tailor spatial conservation measures supporting an Ecosystem-Based Fisheries Management.

Cnidarian toxins represent a rich source of biologically active compounds. Since they may act via oxidative stress events, the aim of the present study was to verify whether crude venom, extracted from the jellyfish Pelagia noctiluca, elicits inflammation and oxidative stress processes, known to be mediated by Reactive Oxygen Species (ROS) production, in rats. In a first set of experiments, the animals were injected with crude venom (at three different doses 6, 30 and 60 µg/kg, suspended in saline solution, i.v.) to test the mortality and possible blood pressure changes. In a second set of experiments, to confirm that Pelagia noctiluca crude venom enhances ROS formation and may contribute to the pathophysiology of inflammation, crude venom-injected animals (30 µg/kg) were also treated with tempol, a powerful antioxidant (100 mg/kg i.p., 30 and 60 min after crude venom). Administration of tempol after crude venom challenge, caused a significant reduction of each parameter related to inflammation. The potential effect of Pelagia noctiluca crude venom in the systemic inflammation process has been here demonstrated, adding novel information about its biological activity.

Historically the majority of Mediterranean trawl fisheries occur on the continental shelf with a smaller proportion focused on the shelf slope and deep sea areas. Understanding how trawl fisheries affect the wider ecosystem is an important component of the ecosystem-based approach to fisheries management. In this context the current study examined the impact of the otter trawl fishery on the infaunal communities found on the continental shelf and upper slope off the coast of Sicily and Calabria, Italy. A total of thirty six sites were sampled across a gradient of fishing intensity and from within a large bay from which trawling has been excluded for 22 years. Fishing intensities were ascertained post-hoc from vessel monitoring system data. Seabed characteristics of the sites studied were uniform across the continental shelf and slope areas that were studied, such that the only factor that varied was fishing intensity. The density index (DI) and total number of species ( S ) were significantly higher in the fishery closure area compared with other continental shelf sites. In particular, bioturbating decapod fauna occurred only within the fishery closure area. Fished sites were dominated primarily by burrowing deposit feeding worms, small bivalves and scavenging biota. In contrast, the response to fishing on the upper slope was less clear. This observation was treated with caution as the power to detect fishing effects was lower for the upper slope sites as a result of possible illegal fishing that had compromised two of the four replicate sites within the closed area. While the present study was able to quantify the effect of the demersal trawl fleet on the benthic infauna of the continental shelf, the effects of trawling on the upper shelf slope remain unclear and warrant further study.

The spatial representation of decapod crustaceans (Crustacea, Malacostraca) of the Strait of Sicily (Central Mediterranean Sea) is presented for the first time, together with comments on their vertical and horizontal distributions. The most relevant trawlable species were analysed using quantitative data gathered in 260 daylight lasting hauls, which covered a large portion (about 100 000 km2) of the investigated area. The index of local abundance, standardized to 1 km2, both in weight (Biomass Index), number (Density Index) and mean body weight were obtained for each species and for all species combined. A spatial representation of the Density Index was produced for all decapod crustaceans combined and for the most frequent species. In particular, the indices were transferred under a GIS system and spatial maps produced by applying the program ArcView™ 9.0. Different interpolators were tested and the most exact interpolator (IDW Inverse Distance Weighting) was chosen. Both historical (i.e., doubts about the presence or identification of the species) and spatial (i.e., differential distribution among different fishing areas) heterogeneity were detected and commented upon. Concerning the trawlable bottoms, very few species (such as Parapenaeus longirostris (Lucas, 1846), Aristaeomorpha foliacea (Risso, 1827), Aristeus antennatus (Risso, 1816), Nephrops norvegicus (Linnaeus, 1758) and Plesionika martia (A. Milne-Edwards, 1883)) are consistently and largely represented in the catches. A high spatial heterogeneity, likely reflecting the complex interaction between fishing pattern, hydrological features and bottom typology, was made evident.

In the Mediterranean sea, about 150 species of invasive macroalgae are recorded until now these alien species have produced serious effect due to their capability to modify the physical and chemical property of the invaded habitats and to compete with native assemblages so as threatening the biodiversity and the ecosystem functioning. Based on morphological characters, the invasive green alga Caulerpa taxifolia var. distichophylla, has been recognised for the first time in the Strait of Messina area, off the northeastern coast of Sicily (Tyrrhenian Sea, western Mediterranean). This record confirms the rapid spread of this taxon from the Levantine area towards the western basins, and suggests a human-mediated dispersion. The new invader occurs in different habitats than C. taxifolia (Vahl) C. Agardh, and the respective habitats do not overlap in the Strait of Messina. A previously undescribed association of this species with tropical–subtropical phanerogams and green algae, is a further example of the global change-mediated reorganisation of Mediterranean benthic assemblages. This record expands the known distribution range of this invasive green algae in Mediterranean Sea.

Among filter-feeders, pennatulids are the most complex and polymorphic members of the cnidarian class Anthozoa. They display a wide distribution throughout all the oceans, constituting a significant component of the sessile megafauna from intertidal to abyssal depths. In this study, a total of 118 bacterial isolates from enrichment cultures, carried out with homogenates of the sea pen Pteroeides spinosum (Ellis, 1764), were screened for hydrocarbon utilization by using the 2,6-dichlorophenol indophenol assay. Among them, 83 hydrocarbon-oxidizing isolates were analyzed for biosurfactant production by standard screening tests (i.e., emulsifying activity, E24 detection, surface tension measurement, microplate assay). The 16S rRNA gene sequencing revealed the affiliation of the most promising isolates to the genera Brevibacterium and Vibrio. Biosurfactant production resulted strongly affected by salinity and temperature conditions, and occurred in the presence of diesel oil and/or crude oil, whereas no production was observed when isolates were grown on tetradecane. The strains resulted able to create stable emulsions, thus suggesting the production of biosurfactants. Further analyses revealed a glycolipidic nature of the biosurfactant extracted from Vibrio sp. PBN295, a genus that has been only recently reported as biosurfactant producer. Results suggest that pennatulids could represent a novel source for the isolation of hydrocarbon-oxidizing bacteria with potential in biosurfactant production.

Persistent organic pollutants (POPs) are known to act as “obesogens”, being fat-soluble and affecting lipid metabolism. The Atlantic bluefin tuna, Thunnus thynnus, are top pelagic predators prone to bioaccumulate and biomagnify environmental contaminants. This study aimed at evaluating POPs-induced ectopic lipid accumulation in liver of adult tuna from the Mediterranean Sea. PCBs and organochlorine pesticides were measured in tuna liver, and marked morphological changes observed, namely poorly compacted tissues, intense vacuolization, erythrocyte infiltration and presence of melanomacrophages. The expression of perilipin, a lipid-droplet marker, positively correlated with the gene expression of PPARγ, a master regulator of adipogenesis, and its heterodimeric partner, RXRα. Changes in metabolites involved in fatty acid biosynthesis and ketogenesis were also observed. Although male bluefin tuna appeared to be more sensitive than females to the adverse effects of environmental obesogens, the alterations observed in tuna liver of both sexes suggest a potential onset of hepatic steatosis.

[A hitherto undescribed association between the comatulid, Antedon mediterranea and the galatheid crab, Munida intermedia, from the Tyrrhenian Sea (Mediterranean Sea), is herein reported.Three pairs of Antedon/Munida were sampled on muddy bottoms at a depth ranging from 10 to 95 m. The crabs collected were too small to establish their sex, and two of them were smaller than previously recorded. The specimens of M. intermedia were found on the external side of the arm base of the host and showed a cryptic coloration pattern. The relationship between A. mediterranea and young M. intermedia is not yet clear. The first seems to be a passive partner, while the latter probably takes advantage of its position on the crinoid's arm. The crab may find protection and shelter, and it may capture planktonic matter or larger living prey. In questa nota si riporta la segnalazione della associazione, ad oggi mai descritta, tra il comatulide Antedon mediterranea e il granchio galateide Munida intermedia, nel Mar Tirreno (Mar Mediterraneo). Sono state collezionate tre coppie di Antedon/Munida provenienti da fondi mobili tra 10 e 95 metri di profondita. I granchi sono risultati troppo piccoli per poterne determinare il sesso e due di essi sono risultati i piu piccoli mai registrati per questa specie. Gli esemplari di M. intermedia sono stati rinvenuti alla base del braccio dell'ospite, sulla parte esterna e presentavano un pattern di colorazione simile al crinoide. Le relazioni che possono intercorrere tra A. mediterranea e giovani di M. intermedia non sono ancora chiare.Quest'ultimo probabilmente trae vantaggio dalla sua posizione sul braccio del crinoide, che invece sembrerebbe un partner passivo. Il granchio puo trovare protezione e riparo e puo catturare materiale organico sospeso o prede vive di maggiori dimensioni., A hitherto undescribed association between the comatulid, Antedon mediterranea and the galatheid crab, Munida intermedia, from the Tyrrhenian Sea (Mediterranean Sea), is herein reported.Three pairs of Antedon/Munida were sampled on muddy bottoms at a depth ranging from 10 to 95 m. The crabs collected were too small to establish their sex, and two of them were smaller than previously recorded. The specimens of M. intermedia were found on the external side of the arm base of the host and showed a cryptic coloration pattern. The relationship between A. mediterranea and young M. intermedia is not yet clear. The first seems to be a passive partner, while the latter probably takes advantage of its position on the crinoid's arm. The crab may find protection and shelter, and it may capture planktonic matter or larger living prey. In questa nota si riporta la segnalazione della associazione, ad oggi mai descritta, tra il comatulide Antedon mediterranea e il granchio galateide Munida intermedia, nel Mar Tirreno (Mar Mediterraneo). Sono state collezionate tre coppie di Antedon/Munida provenienti da fondi mobili tra 10 e 95 metri di profondita. I granchi sono risultati troppo piccoli per poterne determinare il sesso e due di essi sono risultati i piu piccoli mai registrati per questa specie. Gli esemplari di M. intermedia sono stati rinvenuti alla base del braccio dell'ospite, sulla parte esterna e presentavano un pattern di colorazione simile al crinoide. Le relazioni che possono intercorrere tra A. mediterranea e giovani di M. intermedia non sono ancora chiare.Quest'ultimo probabilmente trae vantaggio dalla sua posizione sul braccio del crinoide, che invece sembrerebbe un partner passivo. Il granchio puo trovare protezione e riparo e puo catturare materiale organico sospeso o prede vive di maggiori dimensioni.]

In aquatic environments, bivalve molluscs are used as sentinel species for environmental biomonitoring. In this study Pinna nobilis specimens, the biggest Mediterranean bivalve, were collected in the Magaluf bay (Mallorca), a touristic location and in a pristine area of the Cabrera National Park as the control location. Histological and histochemical analysis in gills of specimens sampled from Magaluf exhibited evident tissue alterations with high presence of haemocytes. Lower acetylcholinesterase (AChE) activity and protein expression were also found in the gills of specimens collected from Magaluf compared with the control area. The determination of antioxidant enzyme activities, such as superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase, showed a higher activities of these antioxidant enzymes and total glutathione content in samples from Magaluf bay than in Cabrera. In conclusion, the present study demonstrated that human activities result in morphological tissue alterations and a reduced AChE activity in gills of P. nobilis. Moreover, these stressful environmental conditions induced an adaptive response in P. nobilis as evidenced by increased antioxidant defences and a decreased AChE activity. CAPSULE: The human activities induce oxidative stress in P. nobilis as evidenced by increased antioxidant defences and a decreased acetylcholinesterase activity., SI

The presence of extensive pennatulacean fields was highlighted in the southern part of the Strait of Messina, Central Mediterranean Sea, during a survey carried out by a Remotely Operated Vehicle (ROV). Pennatulaceans belonging to Pteroeides spinosum were observed from 30 to 160 m. The sea pens reached densities of ≤10 specimens m−2. Their main arrangement patterns are described and their dependence on the interaction between terrigenous organic input and sea-bottom currents is discussed in the light of the ecological importance of sea pen fields.