51. Comparison of adequacy between transbronchial lung cryobiopsy samples and endobronchial ultrasound-guided transbronchial needle aspiration samples for next-generation sequencing analysis
- Author
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Jonsu Minami, Toshio Kumasaka, Nobuyasu Awano, Kazushi Fujimoto, Naoyuki Kuse, Kohei Takada, Yutaka Muto, Takehiro Izumo, Minoru Inomata, and Mari Tone
- Subjects
0301 basic medicine ,Pulmonary and Respiratory Medicine ,Male ,medicine.medical_specialty ,Lung Neoplasms ,cryobiopsy ,next‐generation sequencing ,Tumor cells ,03 medical and health sciences ,0302 clinical medicine ,Bronchoscopy ,medicine ,Humans ,Endobronchial ultrasound ,Lung cancer ,Endoscopic Ultrasound-Guided Fine Needle Aspiration ,Aged ,Retrospective Studies ,Aged, 80 and over ,Lung ,medicine.diagnostic_test ,business.industry ,High-Throughput Nucleotide Sequencing ,Retrospective cohort study ,General Medicine ,Original Articles ,Middle Aged ,medicine.disease ,lung cancer ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,endobronchial ultrasound‐guided transbronchial needle aspiration ,030220 oncology & carcinogenesis ,Female ,Original Article ,Radiology ,business - Abstract
Background Most lung cancer patients present with lesions in both lung fields and lymphadenopathy. Thus, transbronchial lung cryobiopsy (TBLC) and endobronchial ultrasound‐guided transbronchial needle aspiration (EBUS‐TBNA) are commonly performed for diagnosing lung cancer. However, the adequacy of these samples for next‐generation sequencing (NGS) analysis remains unclear. This study aimed to compare the adequacy between TBLC and EBUS‐TBNA samples for NGS analysis. Methods This retrospective cohort study included patients whose lung samples were collected via TBLC or EBUS‐TBNA and analyzed using NGS. Out of 46 genes, the number of genes in TBNA and TBLC samples that could not be assessed via NGS analysis was mainly evaluated. Results A total of 37 patients were included and classified into two groups (TBLC group, n = 18 and TBNA group, n = 19). The mean number of genes that could not be evaluated via NGS analysis was significantly lower in the TBLC group than in the TBNA group (0.9 vs. 10.3, P = 0.024). The median total area of tumor cells in TBLC samples was significantly greater than that in TBNA samples (6.3 [1.6–4.2] vs. 2.6 [0.2–17.3] mm2, P, The adequacy for next generation sequencing (NGS) in transbronchial lung cryobiopsy (TBLC) and endobronchial ultrasound‐guided transbronchial needle aspiration (EBUS‐TBNA) samples remains unclear. We found the mean number of genes which could not be assessed via NGS was significantly lower in TBLC samples than EBUS‐TBNA samples. TBLC could obtain adequate samples with a high concentration of uncrushed tumor cells for NGS.
- Published
- 2020