51. Transmission of Severe Acute Respiratory Syndrome Coronavirus 2 via Close Contact and Respiratory Droplets Among Human Angiotensin-Converting Enzyme 2 Mice
- Author
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Xing Liu, Feifei Qi, Chong Xiao, Zhiguang Xiang, Linlin Bao, Wei Deng, Jing Xue, Kaili Lin, Fengli Li, Hong Gao, Wenjie Zhao, Yanfeng Xu, Shunyi Wang, Pin Yu, Jiangning Liu, Mingya Liu, Guanpeng Wang, Qi Lv, Zhiqi Song, Yunlin Han, Yajin Qu, Chuan Qin, Haisheng Yu, Shuran Gong, and Qiang Wei
- Subjects
0301 basic medicine ,Male ,Time Factors ,aerosol ,Respiratory System ,Anal Canal ,Antibodies, Viral ,Immunoglobulin G ,respiratory droplets ,Mice ,0302 clinical medicine ,Chlorocebus aethiops ,Immunology and Allergy ,030212 general & internal medicine ,Respiratory system ,Lung ,biology ,Brief Report ,Viral Load ,Specific Pathogen-Free Organisms ,medicine.anatomical_structure ,AcademicSubjects/MED00290 ,Infectious Diseases ,Angiotensin-converting enzyme 2 ,RNA, Viral ,Female ,Angiotensin-Converting Enzyme 2 ,Antibody ,Coronavirus Infections ,Viral load ,Risk ,Pneumonia, Viral ,Mice, Transgenic ,SARS-COV-2 ,Peptidyl-Dipeptidase A ,Microbiology ,close contact ,03 medical and health sciences ,Betacoronavirus ,transmission routes ,hACE2 transgenic mice ,Weight Loss ,medicine ,Animals ,Humans ,AcademicSubjects/MED00860 ,Pandemics ,Vero Cells ,Aerosols ,business.industry ,COVID-19 ,Angiotensin-converting enzyme ,030104 developmental biology ,biology.protein ,Vero cell ,Pharynx ,business - Abstract
We simulated 3 transmission modes, including close-contact, respiratory droplets and aerosol routes, in the laboratory. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can be highly transmitted among naive human angiotensin-converting enzyme 2 (hACE2) mice via close contact because 7 of 13 naive hACE2 mice were SARS-CoV-2 antibody seropositive 14 days after being introduced into the same cage with 3 infected-hACE2 mice. For respiratory droplets, SARS-CoV-2 antibodies from 3 of 10 naive hACE2 mice showed seropositivity 14 days after introduction into the same cage with 3 infected-hACE2 mice, separated by grids. In addition, hACE2 mice cannot be experimentally infected via aerosol inoculation until continued up to 25 minutes with high viral concentrations., Severe acute respiratory syndrome coronavirus 2 can be highly transmitted among naive angiotensin-converting enzyme 2 mice via close-contact spreading by respiratory droplets. However, these mice cannot be experimentally infected through aerosol inoculation unless it is continued for 25 minutes with a high viral concentration.
- Published
- 2020
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