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Resveratrol protects cardiomyocytes from oxidative stress through SIRT1 and mitochondrial biogenesis signaling pathways
- Source :
- Biochemical and biophysical research communications. 438(2)
- Publication Year :
- 2013
-
Abstract
- Reactive oxygen species (ROS) is generated by oxidative stress and plays an important role in various cardiac pathologies. The SIRT1 signaling pathway and mitochondrial biogenesis play essential roles in mediating the production of ROS. SIRT1 activated by resveratrol protects cardiomyocytes from oxidative stress, but the exact mechanisms by which SIRT1 prevents oxidative stress, and its relationship with mitochondrial biogenesis, remain unclear. In this study, it was observed that after stimulation with 50μMH2O2 for 6h, H9C2 cells produced excessive ROS and downregulated SIRT1. The mitochondrial protein NDUFA13 was also downregulated by ROS mediated by SIRT1. Resveratrol induced the expression of SIRT1 and mitochondrial genes NDUFA1, NDUFA2, NDUFA13 and Mn-SOD. However, the production of these genes was reversed by SIRT1 inhibitor nicotinamide. These results suggest that resveratrol inhibits ROS generation in cardiomyocytes via SIRT1 and mitochondrial biogenesis signaling pathways.
- Subjects :
- NDUFA13
Mitochondrial DNA
endocrine system diseases
Cell Survival
Biophysics
Mitochondrion
Resveratrol
Biology
medicine.disease_cause
Biochemistry
Antioxidants
Cell Line
chemistry.chemical_compound
Sirtuin 1
Stilbenes
medicine
Animals
Myocytes, Cardiac
Molecular Biology
chemistry.chemical_classification
Reactive oxygen species
food and beverages
NADH Dehydrogenase
Cell Biology
Hydrogen Peroxide
Mitochondrial Turnover
Mitochondria
Rats
enzymes and coenzymes (carbohydrates)
Oxidative Stress
Mitochondrial biogenesis
chemistry
biological phenomena, cell phenomena, and immunity
Signal transduction
Reactive Oxygen Species
hormones, hormone substitutes, and hormone antagonists
Oxidative stress
Signal Transduction
Subjects
Details
- ISSN :
- 10902104
- Volume :
- 438
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....a097b5ba732718f3dc93a89c67c44dc6