247 results on '"Maxime Breban"'
Search Results
52. [Diagnosis of spondyloarthritis: when to think about it?]
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Maxime, Breban
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Arthritis, Rheumatoid ,Spondylarthritis ,Humans - Abstract
Diagnosis of spondyloarthritis: when to think about it? Spondyloarthritis represents the second most frequent chronic inflammatory rheumatism, along with rheumatoid arthritis. It characteristically affects joints with axial distribution predominance. The paucity of physical examination and imaging findings during the first years of evolution frequently exposes to deleterious misdiagnosis. In order to improve its recognition and to optimize its care, it is necessary to seek for characteristic past or present clinical manifestations, the combination of which will contribute to establish the diagnostic conviction. In some instances, therapeutic test(s) using anti-inflammatory drug(s) or even biotherapies could be required to reinforce it, even if all complementary examinations are negative.Diagnostic d’une spondyloarthrite : quand y penser ? Les spondyloarthrites représentent le deuxième rhumatisme inflammatoire chronique le plus fréquent avec la polyarthrite rhumatoïde. Elles se caractérisent par une atteinte articulaire prédominant sur le squelette axial. La discrétion des signes d’examens physique et d’imagerie durant les premières années d’évolution est souvent responsable d’une errance diagnostique préjudiciable. Pour en améliorer la reconnaissance et permettre une prise en charge optimale, il faut s’appuyer sur l’interrogatoire, à la recherche des manifestations caractéristiques, présentes ou passées, dont la combinaison permet d’asseoir la conviction diagnostique. Des tests thérapeutiques par antiinflammatoires, voire biothérapie peuvent permettre de le confirmer, même quand tous les examens complémentaires sont négatifs.
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- 2019
53. Veillonella parvula spondylodiscitis
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Robert Carlier, Maxime Breban, A. Felter, I. Padovano, M. de Laroche, H. Gouze, L. Noussair, E. Salomon, Clara Duran, A. Dinh, Université de Versailles Saint-Quentin-en-Yvelines - UFR Sciences de la santé Simone Veil (UVSQ Santé), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Service de médecine interne [CHU Ambroise Paré], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Ambroise Paré [AP-HP], Hôpital Ambroise Paré [AP-HP], Service de Microbiologie [Garches], and Hôpital Raymond Poincaré [AP-HP]
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Adult ,medicine.medical_specialty ,Discitis ,[SDV]Life Sciences [q-bio] ,Coccus ,Veillonella ,Joint infections ,Veillonella parvula ,03 medical and health sciences ,stomatognathic system ,Internal medicine ,Female patient ,Back pain ,Medicine ,Vertebral osteomyelitis ,Humans ,Risk factor ,0303 health sciences ,Lumbar Vertebrae ,biology ,030306 microbiology ,business.industry ,Osteomyelitis ,biology.organism_classification ,medicine.disease ,3. Good health ,stomatognathic diseases ,Infectious Diseases ,Female ,medicine.symptom ,business ,Gram-Negative Bacterial Infections ,Immunocompetence - Abstract
Objectives Veillonella parvula is an anaerobic Gram-negative coccus rarely involved in bone and joint infections. Patients and method We report the case of a Veillonella parvula vertebral osteomyelitis (VO) in a female patient without any risk factor. Results The 35-year-old patient was immunocompetent and presented with Veillonella parvula VO. She was admitted to hospital for inflammatory lower back pain. The discovertebral sample was positive for Veillonella parvula. Literature data on Veillonella VO is scarce. Reported cases usually occurred in immunocompromised patients. Diagnosis delay can be up to four months. Patients are usually afebrile. Outcome with antimicrobial treatment alone is favorable in half of cases. Other patients must undergo surgery. Conclusions Veillonella VO may occur in immunocompetent patients and have a clinical spectrum of mechanical lower back pain.
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- 2019
54. Three Multicenter, Randomized, Double-Blind, Placebo-Controlled Studies Evaluating the Efficacy and Safety of Ustekinumab in Axial Spondyloarthritis
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Atul Deodhar, Lianne S. Gensler, Joachim Sieper, Michael Clark, Cesar Calderon, Yuhua Wang, Yiying Zhou, Jocelyn H. Leu, Kim Campbell, Kristen Sweet, Diane D. Harrison, Elizabeth C. Hsia, Désirée Heijde, Frederico Ariel, Cecilia Adma Asnal, Alberto Berman, Gustavo Citera, Graciela Rodriguez, Veronica Gabriela Savio, Paul Bird, Hedley Griffiths, David Nicholls, Maureen Rischmueller, Jane Zochling, Kurt De Vlam, Michel Malaise, Adrien Nzeusseu Toukap, Filip Van den Bosch, Johan Vanhoof, Rubens Bonfiglioli, Mauro Keiserman, Antonio Scafuto Scotton, Ricardo Xavier, Antonio Carlos Ximenes, Assen Atanasov, Ivan Goranov, Ivan Kazmin, Rodina Nestorova Licheva, Nikolay Nikolov, Boycho Oparanov, Rumen Stoilov, Louis Bessette, Jude Rodrigues, Ladislav Bortilik, Eva Dokoupilova, Zdenek Dvoarak, Dagmar Galatikova, Petr Nemec, Lucie Podrazilova, Gabriela Simkova, Zuzana Stejfova, Radka Moravcova, Petr Vitek, Alain Cantagrel, Athan Baillet, Beatrice Banneville, Bernard Combe, Maxime Breban, Minh Nguyen, Philippe Goupille, Juergen Braun, Andrea Everding, Joern Kekow, Ramona Koenig, Andrea Rubbert‐Roth, Torsten Witte, Attila Bartha, Edit Drescher, Kata Kerekes, Attila Kovacs, Judit Pulai, Bernadette Rojkovich, Sandor Szanto, Edit Toth, Hilario Avila, Igancio Garcia Torre, Fedra Irazoque, Marco Maradiaga, Cesar Pacheco, Marek Brzosko, Anna Dudek, Slawomir Jeka, Marek Krogulec, Brygida Kwiatkowska, Piotr Wiland, Rafal Wojciechowski, Agnieszka Zielinska, Helena Santos, Olga Bugrova, Valery Christyakov, Vladimir Gorbunov, Elena Ilivanova, Elena Zemerova, Rima Kamalova, Tatyana Kameneva, Galina Macievskaya, Irina Marusenko, Alexey Maslyansky, Svetlana Myasoedova, Leisan Myasoutova, Boris Nemtsov, Olga Nesmeyanova, Tatyana Plaksina, Tatyana Pokrovskaya, Svetlana Polyakova, Andrey Rebrov, Liudmila Savina, Svetlana Smakotina, Marina Stanislav, Olga Ukhanova, Irina Vinogradova, Elena Zonova, Han Joo Baek, Tae‐Hwan Kim, ChangKeun Lee, SangHeon Lee, Sang‐Hoon Lee, Shin‐Seok Lee, Sung‐Hwan Park, YeongWook Song, Chang‐Hee Suh, Juan Amarelo Ramos, Franciso Javier Blanco, Eduardo Collantes, Miguel Consuelo Diaz, Maria Luz Garcia Vivar, Jordi Gratacos, Xavier Juanola, Der‐Yuan Chen, Hsiang‐Cheng Chen, Kun‐Hung Chen, Ying‐Chou Chen, Ying‐Ming Chiu, Shue‐Fen Luo, Shih‐Tzu Tsai, Jui‐Cheng Tseng, Cheng‐Chung Wei, Meng‐Yu Weng, Orest Abrahamovych, Dmytro Reshotko, Oleksandr Golovchenko, Ihor Hospodarsky, Oleg Iaremenko, Olena Levchenko, Oleksandr Dudnyk, Olena Garmish, Olena Grishyna, Galyna Protsenko, Dmytro Rekalov, Svitlana Smiyan, Mykola Stanislavchuk, Svitlana Trypilka, Vira Tseluyko, Samvel Turianytsia, Viktoriya Vasylets, Nataliya Virstyuk, Yaroslav Kleban, Coziana Ciurtin, Karl Gaffney, Wiranthi Gunasekera, Kirsten Mackay, Jon Packham, Raj Sengupta, Hasan Tahir, Jacob Aelion, Ralph Bennett, Julio Gonzalez‐Paoli, Robert M. Griffin, Michael Grisanti, Jyothi Mallepalli, Eric Peters, Joy Schechtman, and Atul Singhal
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0301 basic medicine ,Ankylosing ,Adult ,Male ,medicine.medical_specialty ,Immunology ,Placebo ,Antibodies ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Rheumatology ,Randomized controlled trial ,Double-Blind Method ,law ,Internal medicine ,Ustekinumab ,Monoclonal ,Clinical endpoint ,medicine ,Immunology and Allergy ,Humans ,Spondylitis, Ankylosing ,Adverse effect ,Spondylitis ,030203 arthritis & rheumatology ,Ankylosing spondylitis ,business.industry ,Antibodies, Monoclonal ,Middle Aged ,medicine.disease ,Injection Site Reaction ,Clinical trial ,030104 developmental biology ,Treatment Outcome ,Antirheumatic Agents ,Spondylarthropathies ,Female ,business ,medicine.drug - Abstract
Author(s): Deodhar, Atul; Gensler, Lianne S; Sieper, Joachim; Clark, Michael; Calderon, Cesar; Wang, Yuhua; Zhou, Yiying; Leu, Jocelyn H; Campbell, Kim; Sweet, Kristen; Harrison, Diane D; Hsia, Elizabeth C; van der Heijde, Desiree | Abstract: ObjectiveTo evaluate the efficacy and safety of ustekinumab in 3 randomized, placebo-controlled studies in patients with axial spondyloarthritis (SpA). Studies 1 and 2 included patients with radiographic axial SpA (anti-tumor necrosis factor [anti-TNF]-naive patients and patients with inadequate response or intolerance to anti-TNF, respectively); study 3 patients had nonradiographic axial SpA.MethodsIn all 3 studies, patients were randomly assigned (1:1:1) to receive subcutaneous ustekinumab at 45 mg or 90 mg or placebo up to 24 weeks, after which placebo-treated patients were rerandomized to receive ustekinumab at 45 mg or 90 mg. The primary end point in studies 1 and 2 was the proportion of patients who met the Assessment of SpondyloArthritis international Society criteria for 40% improvement in disease activity (achieved an ASAS40 response). The primary end point in study 3 was the proportion of patients who achieved an ASAS20 response. Other disease activity and safety measures were also evaluated. A week 24 analysis of study 1 was preplanned to determine continuation of studies 2 and 3.ResultsFor study 1, the primary and major secondary end points were not met, and the study was discontinued. As a result, studies 2 and 3 were prematurely discontinued before they were fully enrolled. For all 3 studies, neither ustekinumab dose group demonstrated clinically meaningful improvement over placebo on key efficacy end points. The proportion of patients experiencing adverse events in the ustekinumab groups was consistent with that in previous studies.ConclusionIn these 3 placebo-controlled trials, efficacy of ustekinumab in the treatment of axial SpA was not demonstrated. The safety profile was consistent with that of studies in other indications.
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- 2019
55. Ankylosing Spondylitis and Related Immune-Mediated Disorders
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Maria Teresa Fiorillo, Nigil Haroon, Francesco Ciccia, Maxime Breban, Fiorillo, M. T., Haroon, N., Ciccia, F., and Breban, M.
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lcsh:Immunologic diseases. Allergy ,anti-TNF therapy ,Immunology ,ERAP ,HLA-B27 alleles ,ankylosing spondylitis ,anti-CD74 autoantibodies ,biomarkers ,enthesitis ,functional genomics ,enthesiti ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,medicine ,Interleukin 23 ,functional genomic ,Humans ,Immunology and Allergy ,Spondylitis, Ankylosing ,Axial spondyloarthritis ,Spondylitis ,030304 developmental biology ,0303 health sciences ,Ankylosing spondylitis ,business.industry ,Enthesitis ,Disease Management ,medicine.disease ,anti-CD74 autoantibodie ,3. Good health ,ankylosing spondyliti ,Editorial ,Immune System Diseases ,030220 oncology & carcinogenesis ,biomarker ,Anti-TNF therapy ,Disease Susceptibility ,Interleukin 17 ,medicine.symptom ,lcsh:RC581-607 ,business - Published
- 2019
56. Microbiote intestinal et rhumatismes inflammatoires
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Maxime Breban
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0301 basic medicine ,Gynecology ,03 medical and health sciences ,medicine.medical_specialty ,030104 developmental biology ,Rheumatology ,business.industry ,medicine ,business - Abstract
Resume La cause des maladies inflammatoires chroniques reste dans la plupart des cas meconnue. Parmi les hypotheses possibles, celle d’une infection chronique reste soulevee. L’evolution recente des techniques permet d’etudier de facon approfondie le contenu microbien – ou microbiote – de l’intestin et d’y decrire une grande variete de germes qui sont pour la plupart non cultivables. Les maladies inflammatoires de l’intestin ont ete associees a des perturbations caracteristiques du microbiote intestinal, qui persistent en dehors des periodes inflammatoires et pourraient contribuer a la pathogenie de ces maladies. Les relations entre microbiote intestinal et inflammation articulaire, mises en evidence dans des modeles murins d’arthrite, restent encore peu explorees chez les patients. Des etudes recentes revelent toutefois l’existence de perturbations significatives de ce microbiote aussi bien au cours de la polyarthrite rhumatoide que du rhumatisme psoriasique et des spondyloarthrites, avec des differences selon les pathologies et les etudes. De facon interessante, certaines des anomalies decrites (restriction de la diversite microbienne, reduction de la frequence de groupes bacteriens du phylum des Firmicutes connus pour leurs proprietes immuno-regulatrices) ressemblent a celles maintenant bien etablies au cours des maladies inflammatoires de l’intestin. Les perspectives ouvertes par ces resultats sont importantes aussi bien pour la comprehension de la maladie que pour le developpement de biomarqueurs ou d’approches therapeutiques innovantes.
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- 2016
57. A family-based genome-wide association study reveals an association of spondyloarthritis with MAPK14
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Gilles Chiocchia, Diana Zelenika, Céline Charon, Michael H. Weissman, Roula Said-Nahal, Maxime Breban, Elena Zinovieva, Brigitte Izac, Ariane Leboime, Henri Jean Garchon, Ivo Gut, Félicie Costantino, John D. Reveille, and Alice Talpin
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Adult ,Male ,0301 basic medicine ,Immunology ,Genome-wide association study ,Single-nucleotide polymorphism ,Major histocompatibility complex ,Polymorphism, Single Nucleotide ,General Biochemistry, Genetics and Molecular Biology ,Mitogen-Activated Protein Kinase 14 ,03 medical and health sciences ,0302 clinical medicine ,Rheumatology ,Polymorphism (computer science) ,Spondylarthritis ,medicine ,Humans ,Immunology and Allergy ,Genetic Predisposition to Disease ,Genetic association ,030203 arthritis & rheumatology ,Genetics ,Ankylosing spondylitis ,biology ,business.industry ,Middle Aged ,medicine.disease ,030104 developmental biology ,Cohort ,biology.protein ,Female ,Gene polymorphism ,business ,Genome-Wide Association Study - Abstract
ObjectiveMore than 40 loci have been associated with ankylosing spondylitis (AS), but less is known about genetic associations in spondyloarthritis (SpA) as a whole. We conducted a family-based genome-wide association study (GWAS) to identify new non-major histocompatibility complex (MHC) genetic factors associated with SpA.Methods906 subjects from 156 French multiplex families, including 438 with SpA, were genotyped using Affymetrix 250K microarrays. Association was tested with Unphased. The best-associated non-MHC single nucleotide polymorphisms (SNPs) were then genotyped in two independent familial cohorts (including 215 French and 294 North American patients with SpA, respectively) to replicate associations.Results43 non-MHC SNPs yielded an association signal with SpA in the discovery cohort (p−4). In the extension studies, association was replicated at a nominal p value of pMAPK14, and SpA (p=3.5×10−7). Such association appeared to be independent of HLA-B27.ConclusionsWe report here for the first time a family-based GWAS study on SpA and identified an associated polymorphism near MAPK14. Further analyses are needed to better understand the functional basis of this genetic association.
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- 2016
58. Two Phenotypes Are Identified by Cluster Analysis in Early Inflammatory Back Pain Suggestive of Spondyloarthritis: Results From the DESIR Cohort
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Maria Antonietta D'Agostino, Félicie Costantino, Philippe Aegerter, Maxime Breban, and Maxime Dougados
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030203 arthritis & rheumatology ,Ankylosing spondylitis ,Pathology ,medicine.medical_specialty ,business.industry ,Inflammatory back pain ,Immunology ,Desir cohort ,Disease ,medicine.disease ,Phenotype ,03 medical and health sciences ,0302 clinical medicine ,Rheumatology ,Multiple correspondence analysis ,Internal medicine ,Cohort ,Back pain ,Immunology and Allergy ,Medicine ,030212 general & internal medicine ,medicine.symptom ,business - Abstract
Objective To determine whether disease manifestations at baseline would combine according to distinguishable ordered phenotypes in patients with early inflammatory back pain (IBP) suggestive of spondyloarthritis (SpA). Methods Baseline clinical and demographic characteristics as well as imaging features and biologic data on patients included in the French multicenter Devenir des Spondyloarthropathies Indiffererenciees Recentes cohort were analyzed by multiple correspondence analysis and cluster analysis to identify subgroups of patients based on shared characteristics. Results Cluster analysis allowed us to classify the 679 patients with no missing data into 2 major groups-one with a predominance of isolated axial manifestations and the other with associated peripheral symptoms. The application of the same analysis to selected subsets of the cohort, such as HLA-B27-positive and -negative patients and patients fulfilling the Assessment of SpondyloArthritis international Society classification criteria for axial SpA, resulted again in an optimal division of the samples into 2 recurrent clusters of patients similar to those observed in the whole cohort. Conclusion Cluster analysis of SpA manifestations among patients with early IBP highly suggestive of SpA allowed us to clearly identify at baseline 2 different clinical phenotypes-one with predominant axial manifestations and the other with predominant peripheral manifestations. Ongoing follow-up will allow us to determine whether these clusters correspond to different patterns of disease severity.
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- 2016
59. Présentation clinique des patients souffrant de rachialgie inflammatoire chronique récente évocatrice de spondyloarthrite : la cohorte Desir
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Sophie Bouvet, Bruno Fautrel, Christian Roux, Alain Saraux, Jean-Pierre Daurès, Maxime Breban, Maria Antonietta D'Agostino, Bernard Combe, Pascal Claudepierre, Francis Berenbaum, Sandrine Alonso, Anna Molto, Daniel Wendling, Désirée van der Heijde, Adrien Etcheto, Jean-Marc Tréluyer, Paul Landais, Philippe Goupille, Maxime Dougados, Pascal Richette, Thao Pham, and Antoine Feydy
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Rheumatology - Abstract
Resume Objectifs Desir est une etude de cohorte prospective, longitudinale, multicentrique, francaise de patients souffrant de rachialgie inflammatoire evocatrice de spondyloarthrite, avec un suivi de 10 ans. Le but est d’evaluer les performances des differents systemes de criteres de classification de la spondyloarthrite axiale, et de decrire la frequence et les caracteristiques des signes cliniques de spondyloarthrite axiale. Methodes Les donnees demographiques et les items permettant la classification et le calcul des indices ont ete recueillis, ainsi que les donnees biologiques et d’imagerie. Les donnees a l’inclusion furent analysees. La performance de plusieurs systemes de criteres de classification fut evaluee (LR, rapport de vraisemblance) avec le diagnostic du medecin comme « gold standard ». Concernant la presentation clinique de la spondyloarthrite axiale, une analyse descriptive a ete realisee. Resultats Parmi les patients, 708 sont inclus ; 92 % d’entre eux satisfont a au moins un systeme de criteres de classification : mNY 26 %, 79 % Amor, ESSG 78 %, ASAS 70 % ; le niveau de confiance du medecin est de 6,8 ± 2,7. Parmi les patients, 81 et 83 % remplissent les criteres modifies (y compris IRM) d’Amor ou de l’ESSG. Une atteinte axiale est presente dans 100 % des cas. Les AINS sont pris par 90 % des patients, avec un score d’AINS de 50 ± 46. Un BASDAI superieur a 40 est note dans 60 % des cas et une CRP elevee dans 30 % des cas. L’antigene HLA-B27 est present dans 58 % des cas. Suivant l’ASDAS CRP, 12,7 % des patients ont une maladie inactive, 63 % une activite de la maladie tres elevee ; le BASFI moyen etait de 30. Une atteinte peripherique est presente dans 57 % des cas, avec une arthrite dans 37 % de ceux-ci. Une enthesite est notee chez 49 % des patients, et constitue le premier symptome chez 22,5 % des cas ; la participation de la paroi thoracique anterieure est notee dans 44,6 % des cas, et une dactylite dans 13 %. Parmi les manifestations extra-articulaires supplementaires, le psoriasis est repertorie dans 16 % des cas, une uveite dans 8,5 % et une maladie inflammatoire chronique intestinale (MICI) dans 5,1 %. Le tabagisme est present dans 36,3 % des cas et une hypertension arterielle dans 5,1 % des cas. Conclusion Ces donnees representent la base de l’evaluation du suivi de cette cohorte, permettant de futures etudes specifiques.
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- 2015
60. Genomewide Association Study of Acute Anterior Uveitis Identifies New Susceptibility Loci
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Maria A. Fiatarone Singh, Maxime Breban, Peter McCluskey, Irene van der Horste-Bruinsma, Michael M. Ward, Xiu-Feng Huang, Gareth T. Jones, Matthew A. Brown, Denis Wakefield, Finbar O'Shea, David Hughes, Zhixiu Li, Yorgi Mavros, Philip Robinson, MinJae Lee, Lianne S. Gensler, Gary J. Macfarlane, B. Paul Wordsworth, Michael H. Weisman, Mohammad H. Rahbar, James T. Rosenbaum, Zi-Bing Jin, Erika De Guzman, Eva Klingberg, Paul Leo, Helena Forsblad-d'Elia, Helena Marzo-Ortega, Jeff S. Coombes, John D. Reveille, Nicole Vlahovich, Tammy M. Martin, Amsterdam Movement Sciences, AII - Inflammatory diseases, Rheumatology, and AMS - Tissue Function & Regeneration
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Adult ,Male ,0301 basic medicine ,Genotyping Techniques ,Locus (genetics) ,Single-nucleotide polymorphism ,Genome-wide association study ,heritability ,Biology ,Polymorphism, Single Nucleotide ,03 medical and health sciences ,0302 clinical medicine ,ankylosing spondylitis ,Genetics ,Odds Ratio ,GWAS ,Humans ,SNP ,Genetic Predisposition to Disease ,Spondylitis, Ankylosing ,Genotyping ,030203 arthritis & rheumatology ,genetic risk scores ,acute anterior uveitis ,Odds ratio ,Middle Aged ,Uveitis, Anterior ,030104 developmental biology ,Genetic Loci ,HLA-B Antigens ,Case-Control Studies ,Acute Disease ,Female ,Imputation (genetics) ,Genome-Wide Association Study ,SNP array - Abstract
PURPOSE. Acute anterior uveitis (AAU) is a common intraocular inflammatory disease. AAU occurs in 30% to 50% of patients with ankylosing spondylitis (AS), and both conditions are strongly associated with human leukocyte antigen (HLA)-B27, implying a shared etiology. This study aims to apply genomewide association study (GWAS) to characterize the genetic associations of AAU and their relationship to the genetics of AS. METHODS. We undertook the GWAS analyses in 2752 patients with AS with AAU (cases) and 3836 patients with AS without AAU (controls). There were 7,436,415 single-nucleotide polymorphisms (SNPs) available after SNP microarray genotyping, imputation, and quality-control filtering. RESULTS. We identified one locus associated with AAU at genomewide significance: rs9378248 (P = 2.69 × 10−8, odds ratio [OR] = 0.78), lying close to HLA-B. Suggestive association was observed at 11 additional loci, including previously reported AS loci ERAP1 (rs27529, P = 2.19 × 10−7, OR = 1.22) and NOS2 (rs2274894, P = 8.22 × 10−7, OR = 0.83). Multiple novel suggestive associations were also identified, including MERTK (rs10171979, P = 2.56 × 10−6, OR = 1.20), KIFAP3 (rs508063, P = 5.64 × 10−7, OR = 1.20), CLCN7 (rs67412457, P = 1.33 × 10−6, OR = 1.25), ACAA2 (rs9947182, P = 9.70 × 10−7, OR = 1.37), and 5 intergenic loci. The SNP-based heritability is approximately 0.5 for AS alone, and is much higher (approximately 0.7) for AS with AAU. Consistent with the high heritability, a genomewide polygenic risk score shows strong power in identifying individuals at high risk of either AS with AAU or AS alone. CONCLUSIONS. We report here the first GWAS for AAU and identify new susceptibility loci. Our findings confirm the strong overlap in etiopathogenesis of AAU with AS, and also provide new insights into the genetic basis of AAU.
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- 2020
61. FRI0449 MANAGEMENT AND OUTCOME OF SEPTIC ARTHRITIS OF NATIVE JOINT: A NATIONWIDE SURVEY
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G. Bart, M. Beaufrere, Emmanuel Hoppé, Elisabeth Gervais, C. Darrieutort, Marion Couderc, P. Richebé, Emmanuelle Dernis, R. Brault, M. Ardizzone, Maxime Breban, Edouard Pertuiset, V. Deprez, S. Ottaviani, Pascal Guggenbuhl, L. Pauvele, J.-E. Gottenberg, S. Godot, Adeline Ruyssen-Witrand, Vincent Goëb, R.M. Flipo, S. Derolez, S. Urien, Daniel Wendling, C. Salliot, P. Coquerelle, R. Seror, Jean-Marc Ziza, C. Roux, A. Michaut, Guillaume Coiffier, and Denis Mulleman
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High rate ,medicine.medical_specialty ,business.industry ,General surgery ,Immunology ,Nationwide survey ,medicine.disease ,General Biochemistry, Genetics and Molecular Biology ,Rheumatology ,Current management ,Streptococcus sp ,Bacteremia ,Charlson comorbidity index ,medicine ,Immunology and Allergy ,Septic arthritis ,business ,Joint immobilization - Abstract
Background:Objectives:To describe current management and outcome of septic arthritis on native joint in French rheumatology departments.Methods:Retrospective, nation-wide multicentric study. 127 French rheumatology departments were contacted to report 10 successive cases of septic arthritis on native joint that occurred between the 01/01/16 to 31/12/17 (excluding mycobacteria). Characteristics, diagnosis procedure, therapeutic management and outcome were recorded.Results:52 centers included 363 patients (mean age 64± 18.7 years, mean Charlson comorbidity index 4±3). 28.3% patients had a preexisting arthropathy on affected joint. Monoarthritis was observed in 89.6% patients, knee was the most frequent site (38.9%). The most frequent pathogens wereStaphylococcus sp(50.7%) andStreptococcus sp.(23.3%). Bacteremia was found in 156 (45.1%) patients and endocarditis in only 12 (3.0%). Management was heterogeneous. All patients received antibiotics for a mean duration of 46.7±22 days (including intravenous route: 17.3±15.4 d). An initial monotherapy was administered in 42.3% of patients. Surgical procedure (mostly lavage 70.6%) was performed in 171 (48.3%), joint immobilization in 128 (35.3%) (median duration of 21.7±14.1 days). 94 (29.2%) patients have had serious complications including 29 (9.5%) death. Factors associated with death are reported in the table.Conclusion:This study shows that management of septic arthritis is very heterogenous with a still high rate of morbidity and mortality. We identified age, comorbidities, bacteremia and recent antibiotherapy were associated with mortality. Of note, duration of antibiotics was not. Thus, new guidelines are needed in order to facilitate septic arthritis management.Table:FactorsSurvivor(N=276)Dead(N=29)Univariate analysispAdjusted Odds ratio (95%IC)Multivariate analysispAge65 (16-97)82 (32-98)1,07 (1,03-1,12)< 0.001Charlson’s index1 (0-12)2 (0-9)0,00011,3 (1,05-1,63)0,018Delay before antibiotic initiation8,5 (0-310)5 (0-75)0,04840,99 (0,96-1,02)0,562Corticosteroid in the previous 3 months13,9%33,3%0,01842,56 (0,75-8,74)0,133Bacteriemia42,4%71,4%0,00615,07 (1,4-18,370,013Antibiotics in the previous 3 months26,6%56,6%0,00566,7 (2,04-22,01)0,002Disclosure of Interests:Pauline Richebé: None declared, Sophie Godot: None declared, Guillaume Coiffier: None declared, Pascal GUGGENBUHL: None declared, Denis Mulleman: None declared, Marion Couderc: None declared, Emmanuelle Dernis Speakers bureau: Lilly, Novartis, Valentine Deprez: None declared, Carine Salliot: None declared, Saik Urien: None declared, Rachel Brault: None declared, Adeline Ruyssen-Witrand Grant/research support from: Abbvie, Pfizer, Consultant of: Abbvie, BMS, Lilly, Mylan, Novartis, Pfizer, Sandoz, Sanofi-Genzyme, Emmanuel Hoppe: None declared, Jacques-Eric Gottenberg Grant/research support from: BMS, Pfizer, Consultant of: BMS, Sanofi-Genzyme, UCB, Speakers bureau: Abbvie, Eli Lilly and Co., Roche, Sanofi-Genzyme, UCB, Christian Roux: None declared, Sebastien Ottaviani: None declared, Maxime Breban: None declared, Marie Beaufrere: None declared, Alexia Michaut: None declared, Loic Pauvele: None declared, Christelle Darrieutort: None declared, Daniel Wendling: None declared, Pascal COQUERELLE: None declared, Géraldine Bart: None declared, Elisabeth Gervais: None declared, Vincent Goeb: None declared, Marc Ardizzone: None declared, Edouard Pertuiset: None declared, Sophie Derolez: None declared, Jean Marc Ziza: None declared, René-Marc Flipo Consultant of: Johnson and Johnson, MSD France, Novartis, Sanofi, Speakers bureau: Johnson and Johnson, MSD France, Novartis, Sanofi, Raphaèle Seror Consultant of: BMS UCB Pfizer Roche
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- 2020
62. Preface
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Tejpal, Gill and Maxime, Breban
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Rheumatology - Published
- 2019
63. Patients with ankylosing spondylitis have been breast fed less often than healthy controls: a case–control retrospective study
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S. Guis, M. C. Guzian, Jean Roudier, J Montoya, Nathalie Balandraud, Jean-Pierre Mattei, Maxime Breban, Nathalie C. Lambert, P Suchon, and N B Matta
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Adult ,Male ,0301 basic medicine ,medicine.medical_specialty ,Time Factors ,Adolescent ,Immunology ,Breastfeeding ,General Biochemistry, Genetics and Molecular Biology ,Arthritis, Rheumatoid ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Rheumatology ,Internal medicine ,Epidemiology ,medicine ,Humans ,Immunology and Allergy ,Genetic Predisposition to Disease ,Spondylitis, Ankylosing ,Young adult ,HLA-B27 Antigen ,Aged ,Retrospective Studies ,Aged, 80 and over ,030203 arthritis & rheumatology ,Ankylosing spondylitis ,business.industry ,Siblings ,Retrospective cohort study ,Middle Aged ,medicine.disease ,Bottle Feeding ,Gastrointestinal Microbiome ,Surgery ,Breast Feeding ,030104 developmental biology ,Rheumatoid arthritis ,Female ,business ,Breast feeding - Abstract
Objective Ankylosing spondylitis (AS) is a chronic inflammatory disease affecting the spine and pelvis of young adults. On the HLA-B27 genetic background, the occurrence of AS is influenced by the intestinal microbiota. The goal of our study was to test whether breast feeding, which influences microbiota, can prevent the development of AS. Methods First, 203 patients with HLA-B27-positive AS fulfilling the modified New York criteria were recruited in the Department of Rheumatology, Ste Marguerite hospital in Marseilles. A total of 293 healthy siblings were also recruited to make up a control group within the same families. Second, 280 healthy controls, and 100 patients with rheumatoid arthritis and their siblings were recruited. The data collected were age, gender, number of brothers and sisters, age at disease onset, type and duration of feeding (breast or bottle). Results Patients with AS had been breast fed less often than healthy controls. In families where children were breast fed, the patients with AS were less often breast fed than their healthy siblings (57% vs 72%), giving an OR for AS onset of 0.53 (95% CI (0.36 to 0.77), p value=0.0009). Breast feeding reduced familial prevalence of AS. The frequency of breast feeding was similar in the AS siblings and in the 280 unrelated controls. However, patients with AS were less often breast fed compared with the 280 unrelated controls (OR 0.6, 95% CI (0.42 to 0.89), p Conclusions Our study suggests a breastfeeding-induced protective effect on the occurrence of AS. To our knowledge, this is the first study of breastfeeding history in patients with AS.
- Published
- 2015
64. Inefficacy of ultrasound-guided local injections of autologous conditioned plasma for recent epicondylitis: results of a double-blind placebo-controlled randomized clinical trial with one-year follow-up
- Author
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Maxime Breban, B. Montalvan, Philippe Hardy, Delphine Borgel, Patrick Le Goux, and Shahnaz Klouche
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Male ,medicine.medical_specialty ,Time Factors ,Visual analogue scale ,medicine.medical_treatment ,Placebo ,Injections ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Double-Blind Method ,Rheumatology ,Randomized controlled trial ,law ,medicine ,Tennis elbow ,Humans ,Pharmacology (medical) ,Saline ,Ultrasonography, Interventional ,Retrospective Studies ,030222 orthopedics ,Platelet-Rich Plasma ,business.industry ,Epicondylitis ,Tennis Elbow ,030229 sport sciences ,Middle Aged ,medicine.disease ,Extensor carpi radialis brevis muscle ,Surgery ,Treatment Outcome ,Platelet-rich plasma ,Female ,business ,Follow-Up Studies - Abstract
Objectives The aim was to assess the efficacy of two intra-tendinous injections of platelet-rich plasma (PRP) on epicondylitis of recent evolution (≤3 months). Methods Our study was a double-blind placebo-controlled randomized trial. Two US-guided injections of either PRP (autologous conditioned plasma) or saline solution were performed with an interval of 4 weeks. The exclusion criterion was previous CS infiltration. Patients were monitored by an independent evaluator blinded to treatment at baseline and 1, 3, 6 and 12 months of follow-up. The primary evaluation criterion was the relative improvement from baseline to 6 months in pain score on visual analog scale (0-10). Secondary criteria were the Roles-Maudsley score and the assessment of pain on isometric contraction of extensor carpi radialis brevis and extensor digitorum communis. Results Twenty-five patients were randomly assigned to each group. Three patients in each arm dropped out before 6 months. In both groups, the pain score [mean (s.d.)] decreased significantly between two consecutive visits from 6.8 (0.8) (PRP) and 7 (1) (saline) at baseline to 2.5 (1.6) and 1.6 (1.5) (PRP) and to 2.1 (1.6) and 1.8 (2.1) (saline) at 6 and 12 months, respectively. At 6 months, no statistically significant difference was found between groups for relative improvement in pain score [autologous conditioned plasma: -63.2 (22.4%); saline: -69.7 (25.1%); P = 0.24]. No significant difference was found for the secondary criteria. Conclusion Two US-guided PRP injections for epicondylitis of recent evolution were not more efficacious than saline injections, until 6- and 12-months follow-up. Trial registration ClinicalTrials.gov; https://clinicaltrials.gov/; NCT02378285.
- Published
- 2015
65. Brief Report: Nonsteroidal Antiinflammatory Drug-Sparing Effect of Tumor Necrosis Factor Inhibitors in Early Axial Spondyloarthritis: Results From the DESIR Cohort
- Author
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Anna Moltó, Laure Gossec, Maxime Breban, Benjamin Granger, Maxime Dougados, and Daniel Wendling
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musculoskeletal diseases ,medicine.medical_specialty ,Nonsteroidal ,business.industry ,medicine.medical_treatment ,Immunology ,digestive system diseases ,TNF inhibitor ,Surgery ,chemistry.chemical_compound ,Rheumatology ,chemistry ,Internal medicine ,Propensity score matching ,Cohort ,Immunology and Allergy ,Medicine ,Tumor necrosis factor alpha ,Observational study ,Axial spondyloarthritis ,skin and connective tissue diseases ,business ,Spondylarthropathies - Abstract
Objective To evaluate the effect of tumor necrosis factor (TNF) inhibitors on nonsteroidal antiinflammatory drug (NSAID) intake in a cohort of patients with early axial spondyloarthritis (SpA) over the first 2 years of followup. Methods The Devenir des Spondylarthropathies Indifferenciees Recentes (DESIR) cohort is a prospective, multicenter, observational study cohort of patients with early inflammatory back pain. The management and treatment of these patients were decided by their treating rheumatologists. Data regarding NSAID intake (yes/no) and the Assessment of SpondyloArthritis international Society NSAID score were collected at each visit over 2 years of followup. Patients receiving a TNF inhibitor were matched with those receiving usual care, based on a propensity score. The NSAID-sparing effect of TNF inhibitors was estimated by comparing the percentage of patients reaching several end points (e.g., a decrease in the NSAID score to 50% or to
- Published
- 2015
66. ERAP1Gene Expression Is Influenced by Nonsynonymous Polymorphisms Associated With Predisposition to Spondyloarthritis
- Author
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Zeyna Ka, Alice Talpin, Félicie Costantino, Maxime Breban, Franck Letourneur, Gilles Chiocchia, Amir Kadi, Irini Evnouchidou, Henri-Jean Garchon, Tifenn Leturcq, Ariane Leboime, Peter van Endert, Nelly Bonilla, and Roula Said-Nahal
- Subjects
Genetics ,medicine.medical_specialty ,Lymphoblast ,Immunology ,Haplotype ,Biology ,Molecular biology ,medicine.anatomical_structure ,Rheumatology ,Molecular genetics ,Gene expression ,Genotype ,medicine ,Immunology and Allergy ,Allele ,Gene ,B cell - Abstract
Objective Several polymorphisms in ERAP1 are strongly associated with susceptibility to spondyloarthritis (SpA). The combination of rs17482078, rs10050860, and rs30187 results in the construction of 3 major haplotypes that are associated with SpA (the “protective” haplotype T/T/C, the “neutral” haplotype C/C/C, and the “susceptibility” haplotype C/C/T). The aim of the present study was to determine whether such haplotypes might affect endoplasmic reticulum aminopeptidase 1 (ERAP-1) messenger RNA (mRNA) expression, protein level, and/or enzymatic activity in antigen-presenting cells, a type of cell that is potentially relevant to disease pathogenesis. Methods Monocyte-derived dendritic cells (DCs) were generated in 2 cohorts (a discovery cohort and a replication cohort) comprising a total of 23 SpA patients and 44 healthy controls. Lymphoblastoid B cell lines were established from individuals who were homozygous for the risk, the neutral, or the protective ERAP1 haplotype, respectively. In those samples, we investigated the relationship between ERAP1 haplotypes and mRNA expression level. We also used Western blot analysis to measure the relative protein expression of ERAP-1 and a fluorogenic assay to measure its enzymatic activity. Results In monocyte-derived DCs, there was a strong association between ERAP1 haplotypes and the ERAP-1 mRNA expression level, with higher levels in subjects harboring the susceptibility haplotype (P = 0.001 and P = 5.6 × 10−7 in the discovery and replication cohorts, respectively). In lymphoblastoid B cell lines, we observed a significant correlation between haplotype risk score and ERAP1 transcript or protein level (P = 0.003, ρ = 0.92 for both). Enzymatic activity followed a similar trend both in monocyte-derived DCs and in lymphoblastoid B cell lines. Conclusion These data provide strong evidence that SpA-associated ERAP1 polymorphisms affect the level of gene expression in antigen-presenting cells. How increased production/activity of ERAP-1 may influence susceptibility to SpA remains to be determined.
- Published
- 2015
67. 02.22 Taking fly for understanding the molecular role of hla-b27/hb2m in spondyloarthritis
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Gilles Chiocchia, Benjamin Grandon, Claudine André, Isabelle Guénal, Nadège Jah, Aurore Rincheval-Arnold, Maxime Breban, and Sébastien Gaumer
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Mutation ,Pathology ,medicine.medical_specialty ,biology ,business.industry ,Transgene ,Chromosome ,Human leukocyte antigen ,medicine.disease_cause ,engrailed ,Cell biology ,Chromosome 3 ,medicine ,biology.protein ,Allele ,Antibody ,business - Abstract
Background After more than 40 years of research, the mechanisms underlying the association of HLA-B27 with spondyloarthritis (SpA) remain poorly understood. We hypothesised that Drosophila might be a relevant model to study HLA-B27 and particularly for deciphering the cellular cascade and the genetic pathways affected by HLA-B27 mutation. To do so, we developed HLA-B2705, HLA-B0702 (control) and human Beta-2-microglobulin (hB2m) transgenic Drosophila. Methods Gateway Technology and UAS-Gal4 system used for developing transgenic HLA-B/human hB2m Drosophila. UAS-hB2m transgene was inserted in the long arm of chromosome 3 and UAS-HLA-B2705 and UAS-HLA-B0702 were alternatively inserted at another position in the short arm of the same chromosome. For each construct, various transgenic lines were obtained and crossed with several tissue specific driver strains, to induce the expression of the sequence coding for HLA-B0702 and hB2m or HLA-B2705 and hB2m placed under the control of UAS sequences. Results HLA-B2705/hB2m transgenes were first expressed in Drosophila thanks to the vestigialGa4 driver line allowing to produce targeted proteins in the Vestigial domain (dorso-ventral frontier of the wing epithelia). We observed positive staining with HC10 antibodies (class I heavy chain) and W6/32 antibody (HLA-A, HLA-B and HLA-C conformation) for both tested HLA-B/hB2m but only HLA-B27/hB2m was labelled with ME1 (anti-HLA B/C) antibodies, suggesting a different conformation of HLA-B27 and HLA-B7 with hB2m in the wing epithelia. Furthermore, by mean of the nubbin or engrailed drivers which drive the expression in a larger part of the wing, we observed specific loss of the posterior cross-vein following HLA-B27/hB2m expression but not HLA-B7/hB2m. Conclusion Drosophila lines were established allowing tissue specific expression of different HLA-B alleles. Our data suggest that the expression of HLA-B/hB2m transgenes in epithelial cells leads to a plasma membrane localization for HLA-B2705/hB2m but not for HLA-B0702/hB2m. Furthermore, we observed that tissue specific expression of HLA-B27/hB2m but not HLA-B7/hB2m induced specific loss of cross-vein suggesting it interferes with developmental pathways involved in differentiation. This is the first time that differences in localization between HLA-B2705, a subtype associated with SpA, and HLA-B0702, which is not associated with the disease are reported. These results show that transgenic Drosophila might be a pertinent model to decipher molecular mechanisms involved in HLA-B27 trafficking and to better understand potential different behaviours of HLA-B subtypes.
- Published
- 2017
68. Génétique : les nouveaux éléments
- Author
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Maxime Breban and Félicie Costantino
- Subjects
Rheumatology ,Biology ,Humanities - Abstract
Resume La spondyloarthrite (SpA) est une maladie multifactorielle complexe possedant une forte composante hereditaire, reflet d’un terrain genetique predisposant. Les etudes familiales ont permis d’estimer l’heritabilite de la maladie a plus de 90 % et d’etablir son caractere multigenique. En effet, si l’antigene HLA-B27 est le facteur majeur de susceptibilite a la maladie, expliquant a lui seul 25 a 50 % de la predisposition genetique, plus de 20 autres locus de susceptibilite a la maladie ont ete identifies ces dernieres annees grâce a de vastes etudes d’association pan-genomiques (Genome Wide Association Studies [GWAS]). Un grand nombre de polymorphismes de susceptibilite sont partages entre la SpA et d’autres maladies inflammatoires qui lui sont frequemment associees comme les maladies inflammatoires chroniques de l’intestin ou le psoriasis. Par ailleurs, des etudes genetiques s’interessant a l’ensemble des sous-types de SpA ont permis de confirmer certaines des associations deja mises en evidence dans la spondylarthrite ankylosante (SA), sous-type emblematique de la maladie. L’ensemble de ces elements est en faveur d’un terrain genetique partage entre ces differentes pathologies. Ces decouvertes ont egalement permis d’identifier ou de confirmer de nouvelles voies intervenant dans la survenue de la SpA comme la voie de differenciation lymphocytaire Th17 ou la famille des aminopeptidases. Des etudes fonctionnelles ont egalement demontre les consequences de certains polymorphismes de susceptibilite (comme le polymorphisme rs30187 dans le gene ERAP1 ou rs11209026 dans le gene IL23R ) permettant de mieux comprendre l’implication des locus dans la maladie. Cependant, le polymorphisme causal reste a identifier pour la majorite des locus de susceptibilite. Malgre les decouvertes recentes, l’ensemble des variants identifies a ce jour n’explique qu’une tres faible partie de la predisposition genetique totale et le defi actuel consiste a identifier ce qui constitue encore la part « d’heritabilite manquante ». Le developpement rapide du sequencage haut debit pourrait aider a la decouverte de variants rares, difficiles a identifier par les approches GWAS classiques.
- Published
- 2014
69. Immunopathologie actuelle
- Author
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Maxime Breban
- Subjects
Rheumatology - Published
- 2014
70. Increased Production of Interleukin-17 Over Interleukin-10 by Treg Cells Implicates Inducible Costimulator Molecule in Experimental Spondyloarthritis
- Author
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Muriel Andrieu, Maxime Breban, Quentin Jouhault, Luiza M. Araujo, Gilles Chiocchia, Ingrid Fert, and Karine Labroquere
- Subjects
musculoskeletal diseases ,Cluster of differentiation ,medicine.drug_class ,medicine.medical_treatment ,Transgene ,Immunology ,chemical and pharmacologic phenomena ,hemic and immune systems ,Biology ,Monoclonal antibody ,Phenotype ,Proinflammatory cytokine ,Interleukin 10 ,Cytokine ,Rheumatology ,medicine ,Immunology and Allergy ,Interleukin 17 - Abstract
Objective HLA–B27/human β2-microglobulin (hβ2m)–transgenic (B27-transgenic) rats develop an inflammatory disorder resembling spondyloarthritis, with accumulation of proinflammatory Th17 cells. Because Treg cells and Th17 cells have opposing effects in inflammatory disorders, we sought to determine whether biased expansion of Th17 cells could result from altered Treg cell frequency and/or function in B27-transgenic rats. Methods We characterized the phenotype and function of Treg cells from B27-transgenic rats in comparison with those from control rats, by examining their expression of cell surface markers, suppressive activity, cytokine production, and differentiation pattern. Results In B27-transgenic rats, the preferential accumulation of CD4+ Teff cells over Treg cells was not associated with a defect in Treg cell differentiation or suppressive activity. The expression of Treg cell markers was similar between B27-transgenic and control rats, with the exception of the inducible costimulator (ICOS) molecule, which was overexpressed in B27-transgenic rats. High levels of ICOS are considered to be a hallmark of Treg cells with heightened suppressive activity and interleukin-10 (IL-10) expression. Paradoxically, the production of IL-10 by Treg cells was reduced in B27-transgenic rats, whereas the production of IL-17 was enhanced. Moreover, the addition of anti-ICOS monoclonal antibodies during Treg cell differentiation in the presence of dendritic cells from B27-transgenic rats reversed this cytokine profile, restoring the balance between IL-10 and IL-17 in Treg cells from B27-transgenic rats. Conclusion We observed dysregulated production of IL-10 and IL-17 by Treg cells from B27-transgenic rats, which may contribute to disease development. Moreover, our data highlight a key role for ICOS signaling in the generation of imbalanced production of IL-10 and IL-17 by Treg cells in this experimental model of spondyloarthritis.
- Published
- 2014
71. HLA-B27 Subtype Oligomerization and Intracellular Accumulation Patterns Correlate With Predisposition to Spondyloarthritis
- Author
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Aurélie Noteuil, Adèle Sourisce, Cindy Jeanty, Maxime Breban, N. Jah, Ingrid Fert, Claudine André, and Aurore Wielgosik
- Subjects
musculoskeletal diseases ,Yellow fluorescent protein ,biology ,Immunology ,Human leukocyte antigen ,Transfection ,Major histocompatibility complex ,Molecular biology ,Blot ,Rheumatology ,Complementary DNA ,Unfolded protein response ,biology.protein ,Immunology and Allergy ,Intracellular - Abstract
Objective Mechanisms underlying the striking association of spondyloarthritis (SpA) with the class I major histocompatibility complex molecule HLA–B27 remain poorly understood. SpA-like disease develops spontaneously in B*2705-transgenic rats, in conjunction with high HLA–B27 expression levels. This study was undertaken to examine the effects of increased expression of HLA–B27 alleles that are differentially associated with SpA on oligomerization and intracellular redistribution. Methods HeLa cells were transfected with complementary DNA encoding for HLA–B proteins fused to yellow fluorescent protein and/or Renilla luciferase and harvested at an early phase and a later phase of expression. We monitored HLA–B intracellular trafficking and localization by means of microscopy and live-cell imaging. Bioluminescence resonance energy transfer (BRET) and Western blotting were used to monitor HLA–B oligomerization. Results At low expression levels, BRET signals were similarly elevated for all SpA-associated HLA–B27 alleles tested, but were lower for the nonassociated B*2706. Of note, at higher expression levels, HLA–B27 signals remained steady while signal for HLA–B7 decreased sharply, reaching the level observed for B*2706. This was due at least in part to a decreased oligomer proportion without unfolded protein response outbreak. Such differential behavior was not abrogated by proteasome inhibition. With increased expression, all HLA–B proteins accumulated to a high density in cytoplasmic vesicles with labile form and size. The extent of this phenomenon was closely correlated with the level of association with predisposition to SpA. Conclusion To our knowledge, this is the first report of a correlation between the level of predisposition to SpA conferred by HLA–B27 alleles and their biochemical behavior. These findings open new perspectives for understanding the pathogenicity of HLA–B27.
- Published
- 2014
72. Editorial: Animal Models of Spondyloarthritis: Do They Faithfully Mirror Human Disease?
- Author
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Gilles Chiocchia, Maxime Breban, and Luiza M. Araujo
- Subjects
Pathology ,medicine.medical_specialty ,Human disease ,Rheumatology ,business.industry ,Immunology ,medicine ,MEDLINE ,Immunology and Allergy ,Arthritis ,medicine.disease ,Bioinformatics ,business - Published
- 2014
73. Reverse Interferon Signature Is Characteristic of Antigen-Presenting Cells in Human and Rat Spondyloarthritis
- Author
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Gilles Chiocchia, Maxime Breban, Sébastien Jacques, Robert A. Colbert, Ingrid Fert, Nicolas Cagnard, Judith A. Smith, Joel D. Taurog, Simon Glatigny, Luiza M. Araujo, and Franck Letourneur
- Subjects
musculoskeletal diseases ,medicine.medical_treatment ,Immunology ,Dendritic cell ,Biology ,Transcriptome ,Cytokine ,Rheumatology ,Interferon ,medicine ,Immunology and Allergy ,CXCL10 ,Interferon gamma ,SOCS3 ,Antigen-presenting cell ,medicine.drug - Abstract
Objective In HLA–B27–transgenic rats, the development of a disorder that mimics spondyloarthritis (SpA) is highly correlated with dendritic cell (DC) dysfunction. The present study was undertaken to analyze the underlying mechanisms of this via transcriptome analysis. Methods Transcriptome analysis of ex vivo–purified splenic CD103+CD4+ DCs from B27-transgenic rats and control rats was performed. Transcriptional changes in selected genes were confirmed by quantitative reverse transcriptase–polymerase chain reaction. A meta-analysis of our rat data and published data on gene expression in macrophages from ankylosing spondylitis (AS) patients was further performed. Results Interferon (IFN) signaling was the most significantly affected pathway in DCs from B27-transgenic rats; the majority of genes connected to IFN were underexpressed in B27-transgenic rats as compared to controls. This pattern was already present at disease onset, persisted over time, and was conserved in 2 disease-prone B27-transgenic rat lines. In DCs from B27-transgenic rats, we further found an up-regulation of suppressor of cytokine signaling 3 (which may account for reverse IFN signaling) and a down-regulation of interleukin-27 (a cytokine that opposes Th17 differentiation and promotes Treg cells). The meta-analysis of data on conventional DCs from rats and data on monocyte-derived macrophages from humans revealed 7 IFN-regulated genes that were negatively regulated in both human and rat SpA (i.e., IRF1, STAT1, CXCL9, CXCL10, IFIT3, DDX60, and EPSTI1). Conclusion Our results suggest that expression of HLA–B27 leads to a defect in IFNγ signaling in antigen-presenting cells in both B27-transgenic rats and SpA patients, which may result in Th17 expansion and Treg cell alteration (as shown in B27-transgenic rats) and contribute to disease pathogenesis.
- Published
- 2014
74. Faecal microbiota study reveals specific dysbiosis in spondyloarthritis
- Author
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Ariane Leboime, Maxime Breban, Philippe Langella, Julien Tap, Gilles Chiocchia, Harry Sokol, Jean-Pierre Furet, Roula Said-Nahal, Université Paris Descartes, Sorbonne Paris Cité, INSERM UMR-S 606, Hôpital Lariboisière, Assistance Publique-Hôpitaux de Paris, PRES Sorbonne Paris-Cité, and Université Paris Denis Diderot, Université Paris Diderot - Paris 7 (UPD7), Hôpital Ambroise Paré [AP-HP], Université de Versailles Saint-Quentin-en-Yvelines - Institut d'études culturelles et internationales (UVSQ IECI), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris Saclay (COmUE), CEREST-TC [CHU Saint-Antoine], Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Université Paris Descartes - Paris 5 (UPD5), Microorganismes et physiopathologie intestinale (ERL INSERM U1157 - CNRS UMR 7203), Laboratoire des biomolécules (LBM UMR 7203), Université Pierre et Marie Curie - Paris 6 (UPMC)-Département de Chimie - ENS Paris, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Département de Chimie - ENS Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Cellule Pasteur UPMC, Institut Pasteur [Paris] (IP)-Sorbonne Université (SU), Laboratoire d'excellence Inflamex, Sorbonne Paris Cité, Service de Rhumatologie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Ambroise Paré [AP-HP], MetaGenoPolis, Institut National de la Recherche Agronomique (INRA), CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Département de Chimie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)
- Subjects
0301 basic medicine ,rheumatoid arthritis ,Male ,[SDV]Life Sciences [q-bio] ,Disease ,Gut flora ,Inflammatory bowel disease ,Pathogenesis ,Arthritis, Rheumatoid ,Cohort Studies ,Feces ,0302 clinical medicine ,Ruminococcus gnavus ,RNA, Ribosomal, 16S ,Ruminococcus ,Immunology and Allergy ,HLA-B27 Antigen ,biology ,spondyloarthritis ,Middle Aged ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,[SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system ,Female ,musculoskeletal diseases ,Adult ,Immunology ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Rheumatology ,ankylosing spondylitis ,medicine ,Humans ,Aged ,030203 arthritis & rheumatology ,Ankylosing spondylitis ,Siblings ,Case-control study ,medicine.disease ,biology.organism_classification ,Inflammatory Bowel Diseases ,infection ,Gastrointestinal Microbiome ,030104 developmental biology ,Cross-Sectional Studies ,inflammation ,Case-Control Studies ,Multivariate Analysis ,Dysbiosis ,Spondylarthropathies - Abstract
ObjectiveAltered microbiota composition or dysbiosis is suspected to be implicated in the pathogenesis of chronic inflammatory diseases, such as spondyloarthritis (SpA) and rheumatoid arthritis (RA).Methods16S ribosomal RNA gene sequencing was performed on faecal DNA isolated from stool samples in two consecutive cross-sectional cohorts, each comprising three groups of adult volunteers: SpA, RA and healthy controls (HCs). In the second study, HCs comprised a majority of aged-matched siblings of patients with known HLA-B27 status. Alpha and beta diversities were assessed using QIIME, and comparisons were performed using linear discriminant analysis effect size to examine differences between groups.ResultsIn both cohorts, dysbiosis was evidenced in SpA and RA, as compared with HCs, and was disease specific. A restriction of microbiota biodiversity was detected in both disease groups. The most striking change was a twofold to threefold increased abundance of Ruminococcus gnavus in SpA, as compared with both RA and HCs that was significant in both studies and positively correlated with disease activity in patients having a history of inflammatory bowel disease (IBD). Among HCs, significant difference in microbiota composition were also detected between HLA-B27+ and HLA-B27 negative siblings, suggesting that genetic background may influence gut microbiota composition.ConclusionOur results suggest that distinctive dysbiosis characterise both SpA and RA and evidence a reproducible increase in R. gnavus that appears specific for SpA and a marker of disease activity. This observation is consistent with the known proinflammatory role of this bacteria and its association with IBD. It may provide an explanation for the link that exists between SpA and IBD.
- Published
- 2016
75. Hommage à Gilles Chiocchia
- Author
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Maxime Breban
- Subjects
Rheumatology - Published
- 2018
76. Value of Contrast-Enhanced Ultrasonography for the Detection and Quantification of Enthesitis Vascularization in Patients With Spondyloarthritis
- Author
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Philippe Aegerter, Maxime Breban, Maria Antonietta D'Agostino, Gaël Mouterde, and Jean-Michel Correas
- Subjects
medicine.medical_specialty ,Nonsteroidal ,business.industry ,Ultrasound ,Enthesitis ,Enthesis ,Power doppler ,chemistry.chemical_compound ,Rheumatology ,chemistry ,medicine ,Microbubbles ,In patient ,Radiology ,Ultrasonography ,medicine.symptom ,business - Abstract
Objective To evaluate if contrast-enhanced ultrasound (CEUS) can improve the detection and quantification of the vascularization of mild enthesitis in spondyloarthritis (SpA) and to evaluate the influence of nonsteroidal antiinflammatory drugs (NSAIDs) on such detection. Methods Fourteen patients with mildly active SpA were evaluated at 3 consecutive visits: at baseline while undergoing NSAID treatment (V1), after 1 week of stopping NSAIDs (V2), and after 1 week of resuming NSAIDs (V3). At each visit, enthesitis was evaluated clinically and by power Doppler US (PDUS). A selected enthesis with a doubtful PDUS vascularization signal was studied by CEUS in 2 steps: 1) using a dedicated technology that preserves microbubbles (Contrast Tuned Imaging technology [CEUS-CnTI]) and 2) using high PD (CEUS-PD) to destroy microbubbles. A linear mixed model statistical analysis, taking visits and contrast agent as fixed factors and the patient as a random factor, was used. Results Disease activity and PDUS findings increased between V1 and V2 and then decreased between V2 and V3. As compared with PDUS alone, CEUS-PD and CEUS-CnTI each detected 1 supplementary vascularized enthesis at V1, CEUS-PD detected 1 vascularized enthesis and CEUS-CnTI detected 3 vascularized entheses at V2, and CEUS-PD and CEUS-CnTI each detected 2 vascularized entheses at V3. The mean inflammation score was increased by the use of CEUS (P = 0.04). This score increased between V1 and V2 (P = 0.03 by CEUS-PD and P = 0.01 by CEUS-CnTI) and decreased between V2 and V3. Conclusion CEUS improved the detection of enthesitis in SpA patients by confirming all doubtful enthesitis signals and confirming the absence of enthesis vascularization. The use of NSAIDs influenced the detection of vascularization.
- Published
- 2013
77. Prevalence of spondyloarthritis in reference to HLA-B27 in the French population: results of the GAZEL cohort
- Author
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Gilles Chiocchia, Joseph Henny, Marcel Goldberg, Marie Zins, Félicie Costantino, Roula Said-Nahal, Maxime Breban, Henri-Jean Garchon, and Alice Talpin
- Subjects
Male ,musculoskeletal diseases ,medicine.medical_specialty ,Immunology ,Population ,White People ,General Biochemistry, Genetics and Molecular Biology ,Cohort Studies ,Psoriatic arthritis ,Rheumatology ,Internal medicine ,Spondylarthritis ,Epidemiology ,Prevalence ,medicine ,Humans ,Immunology and Allergy ,Genetic Predisposition to Disease ,education ,HLA-B27 Antigen ,Aged ,Ankylosing spondylitis ,HLA-B27 ,education.field_of_study ,business.industry ,Arthritis, Psoriatic ,Middle Aged ,medicine.disease ,Relative risk ,Cohort ,Physical therapy ,Female ,France ,business ,Cohort study - Abstract
Objective To estimate the prevalence of spondyloarthritis (SpA) in reference to HLA-B27 in the French population. Methods In 1989, 20 625 employees of the French national gas and electricity company aged 35–50 years were enrolled in the GAZEL cohort. In 2010, 18 757 still active participants were screened by a questionnaire validated for the detection of SpA. Responders with available DNA were retained for further studies. Pelvic radiograph and HLA-B27 typing were performed in all the self-reported cases of SpA or psoriatic arthritis. Self-reported diagnosis was verified by a qualified rheumatologist. HLA-B27 determination was also performed in subjects without any SpA feature. Results The target population consisted of 6556 responders with available DNA. Their male:female ratio was 3.6 and their mean age was 65.5±3.3 years. A diagnosis of SpA was confirmed in 32 of the 72 self-reported cases, 75% of them being HLA-B27 positive. Estimated SpA prevalence adjusted for sex was 0.43% (95% CI 0.26% to 0.70%). HLA-B27 positivity rate in 2466 healthy controls was 6.9% (95% CI 5.9% to 7.9%). The relative risk of SpA in HLA-B27 positive individuals was 39 (95% CI 17 to 86). Conclusions We estimated the prevalence of SpA in the French population in 2010 to 0.43%. With an estimated prevalence of 75.0% in SpA and 6.9% in healthy controls, HLA-B27 increased the disease risk 39-fold, as compared with HLA-B27 negative subjects.
- Published
- 2013
78. Influence of Environmental Factors on Disease Activity in Spondyloarthritis: A Prospective Cohort Study
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Roula Said Nahal, Maxime Breban, Jean François Vibert, Pierre Yves Boelle, Antoine Flahault, Thomas Hanslik, Emmanuelle Durand, Clément Turbelin, Alfred Mahr, Maria Antonietta D'agostino, Nadine Zeboulon-Ktorza, Homa Madrakian, and Odile Launay
- Subjects
Adult ,Male ,Questionnaires ,medicine.medical_specialty ,Settore MED/16 - REUMATOLOGIA ,Epidemiology ,Immunology ,Pain ,Environment ,Stress ,Severity of Illness Index ,Life Change Events ,Disease activity ,Diagnostic Self Evaluation ,Rheumatology ,Surveys and Questionnaires ,Stress, Psychological/*physiopathology ,Internal medicine ,Spondyloarthritis ,Spondylarthritis ,Humans ,Immunology and Allergy ,Medicine ,Prospective Studies ,Prospective cohort study ,BASDAI ,Generalized estimating equation ,Spondylarthritis/*physiopathology ,Pain Measurement ,Environmental Exposure ,Ankylosing spondylitis ,business.industry ,Repeated measures design ,Middle Aged ,medicine.disease ,Cohort ,Physical therapy ,Psychological ,Female ,Pain/physiopathology ,Life-change events ,BASFI ,business ,Stress, Psychological - Abstract
Objective.Spondyloarthritis (SpA) is a complex inflammatory disorder. We investigated the influence of environmental factors on SpA disease activity.Methods.A prospective cohort of adults with SpA was followed for 3 years. Patients logged on to a secured Website every 3 months to complete a questionnaire. They reported whether they had been exposed to environmental factors such as stressful or traumatic life events, infections, or vaccinations. Outcome variables included the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), the Bath Ankylosing Spondylitis Functional Index (BASFI), and pain and patient global assessment (PGA) on visual numerical scales (each rated 0–10). Analyses were performed using a generalized estimating equation for repeated measures, adjusted for the outcome variable collected by the previous questionnaire.Results.In total, 272 patients were included in the analysis, completing the questionnaire on 2240 occasions. The average time (mean ± SD) between 2 connections to the Website was 4.0 ± 2.0 months. Occurrence of life events was followed by an increase of 0.5 (95% CI 0.4–0.7) in the BASDAI, 0.5 (95% CI 0.3–0.6) in the BASFI, 0.7 (95% CI 0.5–0.9) in the PGA, and 0.8 (95% CI 0.6–1.0) for pain (p < 0.0001 for all variations). A moderately statistically significant link was found between vaccination and an elevation of the BASDAI of 0.3 (95% CI 0.0–0.5; p = 0.032). No influence of other factors was detected.Conclusion.This prospective study in a dedicated SpA cohort shows for the first time a link between stressful events and disease activity. Although this link was statistically highly significant, its clinical meaning remains to be determined because the average magnitude of variation of the different variables studied was rather mild.
- Published
- 2013
79. Are spondylarthritides related but distinct conditions or a single disease with a heterogeneous phenotype?
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Georg Schett, Maxime Breban, Joachim Sieper, Rik Lories, and Dominique Baeten
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business.industry ,Immunology ,Computational biology ,Disease ,Phenotype ,Spine ,Disease Models, Animal ,Genetic Heterogeneity ,Text mining ,Rheumatology ,Risk Factors ,Spondylarthritis ,Animals ,Humans ,Immunology and Allergy ,Medicine ,Spondylarthritides ,Pharmacology (medical) ,business - Published
- 2013
80. Efficacy of rituximab in systemic manifestations of primary Sjögren's syndrome: results in 78 patients of the AutoImmune and Rituximab registry
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Gaël Cinquetti, Eric Hachulla, Philippe Ravaud, Guy Kaplanski, Carine Salliot, Laurent Chiche, Edouard Pertuiset, S. Rist, Jean-Marie Berthelot, Adeline Deplas, Xavier Mariette, Muriel Piperno, Charles Ghiringhelli, Jean Leone, Olivier Meyer, Jacques-Eric Gottenberg, Philippe Gaudin, Charles Zarnitsky, Pascal Richette, Thierry Lequerré, Mohamed Hamidou, Maxime Breban, Christian Marcelli, Jean Sibilia, Philippe Goupille, Claire Larroche, Bernard Combe, Séverine Feuillet, Bruno Gombert, and Philippe Carli
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Adult ,Male ,medicine.medical_specialty ,Immunology ,Disease ,medicine.disease_cause ,General Biochemistry, Genetics and Molecular Biology ,Autoimmunity ,Disease activity ,Antibodies, Monoclonal, Murine-Derived ,Rheumatology ,Internal medicine ,medicine ,Humans ,Immunology and Allergy ,In patient ,Prospective Studies ,Registries ,Prospective cohort study ,Aged ,Aged, 80 and over ,business.industry ,Middle Aged ,Sjogren's Syndrome ,Treatment Outcome ,Antirheumatic Agents ,Female ,Rituximab ,Sjogren s ,business ,Follow-Up Studies ,medicine.drug - Abstract
ObjectivesTo evaluate the efficacy and safety of rituximab in patients with primary Sjögren's syndrome (pSS).MethodsThe AutoImmune and Rituximab registry has included 86 patients with pSS treated with rituximab, prospectivey followed up every 6 months for 5 years.ResultsSeventy-eight patients with pSS (11 men, 67 women), who already had at least one follow-up visit, were analysed. Median age was 59.8 years (29–83), median duration of disease was 11.9 years (3–32). Indications for treatment were systemic involvement for 74 patients and only severe glandular involvement in four patients. The median European Sjögren's Syndrome disease activity index (ESSDAI) was 11 (2–31). 17 patients were concomitantly treated with another immunosuppressant agent. Median follow-up was 34.9 months (6–81.4) (226 patient-years). Overall efficacy according to the treating physician was observed in 47 patients (60%) after the first cycle of rituximab. Median ESSDAI decreased from 11 (2–31) to 7.5 (0–26) (pConclusionsIn common practice, the use of rituximab in pSS is mostly restricted to patients with systemic involvement. This prospective study shows good efficacy and tolerance of rituximab in patients with pSS and systemic involvement.
- Published
- 2012
81. Pouvons-nous améliorer le diagnostic de spondylarthropathie chez les patients ayant un diagnostic incertain ? Présentation de la cohorte prospective multicentrique française EchoSpA
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Sandrine Guis, Anne-Christine Rat, O. Judet, Marie-Agnès Timsit, Alain Saraux, R. Said-Nahal, Isabelle Chary-Valckenaere, Christian Marcelli, Bertrand Lecoq, Alain Blum, Christophe Chagnaud, Philippe Aegerter, Dominique Monnet, Laurent Grange, M. C. Guzian, Damien Loeuille, Philippe Gaudin, Maxime Breban, Sandrine Jousse-Joulin, Ariane Leboime, M.A. D'Agostino, and C. Hacquard-Bouder
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Rheumatology - Abstract
Resume L’echographie par Power Doppler a ultrasons (PDUS) ou Doppler puissance a prouve sa sensibilite comme instrument pour evaluer les enthesites dans les spondylarthropathies (SpA). Chez les patients suspects de SpA, le diagnostic peut etre ameliore par la detection des enthesites par le PDUS. Objectifs Evaluer la performance du PDUS pour le diagnostic des SpA, seul ou combine avec les autres resultats cliniques, biologiques ou radiologiques chez les patients consultant pour une suspicion de SpA. Methodes Etude prospective, multicentrique, d’une cohorte francaise (Boulogne-Billancourt, Caen, Grenoble, Marseille et Nancy). Les patients ont ete recrutes en externe et suivis pendant au moins deux ans pour des symptomes suggerant une SpA (rachialgie inflammatoire (RI), arthrite ou arthralgie inflammatoire (AI), enthesite ou dactylite (ED), uveite B 27 positif (B27+U), antecedents familiaux de SpA (fam). La taille de l’echantillon a ete fixee a 500 patients (pour une prevalence estimee de SpA de 30 ± 5 % apres deux ans). Les patients ont ete soumis a un examen clinique standardise, radiographies du bassin, IRM. des articulations sacro-iliaques, typage HLA et tout autre examen juge utile pour le diagnostic. Pour chaque patient, un examen par PDUS en aveugle des 14 sites enthesitiques a ete effectue a l’inclusion et dans l’annee 1 et 2. Il est prevu de suivre les patients pendant cinq ans. Le diagnostic de SpA affirme par un comite d’experts ignorant les resultats du PDUS, apres au moins deux ans de suivi avec une reevaluation des patients douteux a cinq ans, sera utilise comme « gold standard » pour evaluer les performances diagnostiques du PDUS ainsi que la meilleure procedure en combinant les symptomes cliniques et les autres examens. Resultats Entre janvier 2005 et septembre 2007, 489 patients ont ete inclus (96 % de la population cible). Dix-neuf patients (0,2 %) ont retire leur consentement ou ont ete perdus de vue immediatement apres leur inclusion. A l’inclusion, la moyenne des 470 restants etait de 40 ans, la duree moyenne des symptomes de 6,1 ans ; 42 % d’entre eux etaient B27 positifs et 63 % etaient de sexe feminin. Le critere d’inclusion primaire etait une RI dans 53 %, une AI dans 27 %, une ED dans 9 %, U B27+ dans 8 % et fam dans 4 %. Le suivi est toujours en cours. Conclusion Nous avons mis en place une cohorte unique a visee diagnostique qui inclut l’eventail complet des manifestations de la SpA. En utilisant le PDUS nous nous attendons a ameliorer la procedure du diagnostic des SpA.
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- 2012
82. Immune mechanisms: adaptive immunity
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Maxime Breban and Hill Gaston
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The role of adaptive immunity (i.e. the involvement of B and T lymphocytes) in the pathogenesis of axial spondyloarthritis has been investigated in both human disease and relevant animal models. Studies of B cell responses have not generally implicated an autoantibody in the disease, but there are abnormalities of antibody responses, particularly increased titres of antibodies to various gut bacteria. T cells are critical to the disease in animal models other than those where overexpression of a cytokine is engineered, suggesting that they are the drivers of the inflammatory response. There is convergent evidence from animal models, genetics in humans, and direct observation of human peripheral blood and joints to implicate T cells producing IL-17 under the influence of IL-23. These in turn may be responding to bacteria either in the gut or on the skin.
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- 2016
83. HLA-B27 heavy chain homodimers are expressed in HLA-B27 transgenic rodent models of spondyloarthritis and are ligands for paired Ig-like receptors
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Helen C. Bodmer, Maxime Breban, Lucy A. Bird, Cécile Hacquard-Bouder, Paul Bowness, Andrew J. McMichael, Hiromi Kubagawa, Matthew Roddis, and Simon Kollnberger
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musculoskeletal diseases ,medicine.drug_class ,Transgene ,Recombinant Fusion Proteins ,Immunology ,Cell ,Mice, Transgenic ,Biology ,Monoclonal antibody ,Ligands ,Transfection ,Cell Line ,Animals, Genetically Modified ,Mice ,Biopolymers ,Spondylarthritis ,medicine ,Immunology and Allergy ,Animals ,Biotinylation ,Spondylitis, Ankylosing ,Receptors, Immunologic ,skin and connective tissue diseases ,Receptor ,HLA-B27 Antigen ,Mice, Knockout ,Tumor Necrosis Factor-alpha ,Macrophages ,Antibodies, Monoclonal ,Dendritic Cells ,Molecular biology ,Precipitin Tests ,Lymphocyte Subsets ,Rats ,Disease Models, Animal ,medicine.anatomical_structure ,Cell culture ,biology.protein ,Tumor necrosis factor alpha ,Antibody ,beta 2-Microglobulin ,Dimerization ,Protein Binding - Abstract
HLA-B27 transgenic rats and strains of HLA-B27-transgenic β2-microglobulin (β2m)-deficient mice develop a multisystem inflammatory disease affecting the joints, skin, and bowel with strong similarity to human spondyloarthritis. We show that HLA-B27 transgenic mice and rats express HC10-reactive, β2m-free HLA-B27 homodimers (B272) and multimers, both intracellularly and at the cell surface of leukocytes, including rat dendritic cells. Fluorescent-labeled tetrameric complexes of HLA-B27 homodimers (B272 tetramers) bind to populations of lymphocytes, monocytes, and dendritic cells. The murine (and probably rat) paired Ig-like receptors (PIRs) are ligands for B272. Thus, B272 tetramers stain RBL cells transfected with murine activating PIR-A4 and inhibitory PIR-B receptors. Murine PIR-A and -B can be immunoprecipitated from the RAW264.7 macrophage cell line, and murine PIR-A can be immunoprecipitated from the J774.A1 line using B272. B272 tetramer staining corresponds to the distribution of PIR expression on lymphoid and myeloid cells and on murine macrophage cell lines. B272 can induce TNF-α release from the J774.A1 macrophage cell line. The binding of B272 to PIR is inhibited by HC10, an mAb that ameliorates arthritis in HLA-B27+ β2m−/− mice. The expression and PIR recognition of B272 could explain the pathogenesis of rodent spondyloarthritis.
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- 2016
84. Gut microbiota and inflammatory joint diseases
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Maxime Breban
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0301 basic medicine ,Male ,Firmicutes ,Arthritis ,Disease ,Gut flora ,digestive system ,Inflammatory bowel disease ,Risk Assessment ,Sensitivity and Specificity ,Arthritis, Rheumatoid ,03 medical and health sciences ,Psoriatic arthritis ,0302 clinical medicine ,Rheumatology ,Spondylarthritis ,medicine ,Humans ,030203 arthritis & rheumatology ,biology ,Incidence ,Gastrointestinal Microbiome ,Arthritis, Psoriatic ,medicine.disease ,biology.organism_classification ,Prognosis ,Chronic infection ,030104 developmental biology ,Immunology ,Chronic Disease ,Female - Abstract
In most chronic inflammatory diseases, the cause remains unknown. Chronic infection is, however, among the current hypotheses. Recent technological advances have allowed in-depth studies of the gut microflora, or microbiota, which contains a vast array of organisms, most of which cannot be cultured. Inflammatory bowel disease has been associated with distinctive changes in the gut microbiota, which persist between disease flares and may play a pathogenic role. Links have been demonstrated between the gut microbiota and joint inflammation in murine models of arthritis but have received little attention in human patients. Recent work has nevertheless demonstrated substantial alterations in the gut microbiota in patients with rheumatoid arthritis, psoriatic arthritis, or spondyloarthritis, with differences across diagnoses and studies. Interestingly, some of these alterations resemble those now firmly established in inflammatory bowel disease; examples include decreased microbial diversity and lower frequencies of bacterial groups belonging to the Firmicutes phylum known to have immunoregulatory properties. These new findings open up important new horizons both for understanding disease and for developing novel biomarkers and treatment strategies.
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- 2016
85. Prevalence of ultrasound synovial inflammatory findings in healthy subjects
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Ilaria Padovano, Félicie Costantino, Maria Antonietta D'Agostino, and Maxime Breban
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musculoskeletal diseases ,Adult ,Male ,medicine.medical_specialty ,Pathology ,Settore MED/16 - REUMATOLOGIA ,Adolescent ,Inflammatory arthritis ,Immunology ,Gastroenterology ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Power doppler ,Young Adult ,0302 clinical medicine ,Rheumatology ,Internal medicine ,Synovitis ,medicine ,Prevalence ,Immunology and Allergy ,Humans ,030212 general & internal medicine ,Pathological ,Ultrasonography ,Aged ,030203 arthritis & rheumatology ,Inflammation ,business.industry ,Arthritis ,Ultrasound ,Healthy subjects ,Ultrasonography, Doppler ,Middle Aged ,medicine.disease ,Healthy Volunteers ,Female ,Joints ,Abnormality ,business ,Grading scale - Abstract
Objective To evaluate the prevalence of joint inflammatory abnormalities and erosions detected by grey-scale and Doppler ultrasound (US) in the small joints of hands and feet in healthy subjects. Methods US of the dorsal surface of 32 joints (10 metacarpophalangeal, 10 proximal interphalangeal, 10 metatarsophalangeal (MTP) and 2 wrists) was performed in 207 healthy subjects without joint symptom. Synovial effusion (SE), synovial hypertrophy (SH) and power Doppler (PD) signal were scored using a semiquantitative grading scale (0–3) and erosion binary. Results One-hundred and eighty-two subjects had at least one US abnormality: 52% of the subjects had SE alone, 13% SH alone (5% with and 8% without PD) and 35% both SH and SE. US findings were detected in 9% of the total joints examined, mostly in the feet, and in particular in the MTP1 (33% of the positive joints). SE was the most frequently detected finding (68% of the positive joints), followed by SH (31%). Severity was mild (grade 1 in average) whatever the finding recorded (SH, SE or PD). Four erosions were detected (MTP1). Conclusions This study describes for the first time, in a large cohort of healthy subjects, the prevalence and location of US signs of joint inflammation and of structural damage in small joints of hands and feet. US abnormalities were quite common, and mostly located in the feet. Further studies are needed to define which US components may allow to discriminate between pathological and physiological findings in the joints commonly affected by inflammatory arthritis conditions.
- Published
- 2016
86. Association between the IL-1 family gene cluster and spondyloarthritis
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Nicolas Lebrun, Maxime Breban, Amir Kadi, Dominique Monnet, Gilles Chiocchia, Franck Letourneur, Brigitte Izac, Elena Zinovieva, and Roula Said-Nahal
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Linkage disequilibrium ,Interleukin-1beta ,Immunology ,Locus (genetics) ,Polymorphism, Single Nucleotide ,Linkage Disequilibrium ,General Biochemistry, Genetics and Molecular Biology ,Rheumatology ,Interleukin-1alpha ,Gene cluster ,Humans ,Immunology and Allergy ,Medicine ,SNP ,Spondylitis, Ankylosing ,Genetic Association Studies ,Family Health ,Genetics ,business.industry ,Haplotype ,Case-control study ,Interleukin 1 Receptor Antagonist Protein ,Variable number tandem repeat ,Haplotypes ,IL1A ,Case-Control Studies ,Multigene Family ,business ,Interleukin-1 - Abstract
Objective Spondyloarthritis is a group of articular disorders sharing a genetic background. Polymorphisms in the IL-1 gene cluster have previously been associated with ankylosing spondylitis (AS), a subset of spondyloarthritis. This study examined the association between several of these polymorphisms and the whole spondyloarthritis. Particular attention was devoted to genotype–phenotype correlations. Methods Seven single-nucleotide polymorphisms (SNP) and a variable number tandem repeat located in the IL-1 gene cluster were genotyped in 185 independent spondyloarthritis trios. Family-based association test (FBAT) was computed using the FBAT software. Analysis was carried in spondyloarthritis as a whole and also in AS. A case–control replication study was performed for four of the SNP, in an independent sample of 414 spondyloarthritis and 264 controls. A combined analysis of both studies was performed. Results The SNP rs2856836 in IL1A was significantly associated with spondyloarthritis (p=0.009) and AS (p=0.010) in the family study. The case–control study revealed an association between another IL1A variant (rs1894399) and AS (p=0.035), and between IL1F10.3 (rs3811058) and spondyloarthritis (p=0.041). By combining family and case–control studies an association between AS and IL1A was confirmed (rs1894399, p=0.024), whereas non-AS was more significantly associated with IL1F10.3 (p=0.0043). Family-based and case–control studies revealed significant association between the two most frequent haplotypes combining the four SNP of the replication study and both spondyloarthritis (p=0.0054 and p=0.038) and AS phenotypes (p=0.018 and 0.0036). Conclusion This study is the first to demonstrate an association between several polymorphisms located in the IL-1 gene cluster and spondyloarthritis as a whole. The IL1A locus was strongly associated with AS phenotype, whereas IL1F10 was associated with non-AS.
- Published
- 2012
87. Prevalence, Comorbidities and Burden of Severe Spondyloarthritis In France: Analysis of A National Public Health Insurance Database In 2012 In France
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C. Laurendeau, Julie Gourmelen, Francis Fagnani, Maxime Breban, Jean-Michel Joubert, P. Claudepierre, and T. de Chalus
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business.industry ,Public health insurance ,Environmental health ,Health Policy ,Public Health, Environmental and Occupational Health ,Medicine ,business - Published
- 2015
- Full Text
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88. Dermatite neutrophilique rhumatoïde
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Thierry Clérici, Gilles Hayem, Félicie Costantino, Raphaël André, Marie-Florence de Maleissye, Pierre Sohier, and Maxime Breban
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030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Rheumatology - Abstract
Revue du rhumatisme - In Press.Proof corrected by the author Available online since mercredi 11 janvier 2017
- Published
- 2017
89. The DESIR cohort: A 10-year follow-up of early inflammatory back pain in France: Study design and baseline characteristics of the 708 recruited patients
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Christian Roux, Isabelle Logeart, Alain Saraux, Maxime Dougados, Joelle Benessiano, Francis Berenbaum, Maria Antonietta D'Agostino, Jean-Marc Tréluyer, Pascal Claudepierre, Patricia Dargent-Molina, Véronique Leblanc, Philippe Goupille, Pascal Richette, Antoine Feydy, Martin Rudwaleit, Jean-Pierre Daurès, Maxime Breban, D. Wendling, Désirée van der Heijde, Bruno Fautrel, Bernard Combe, Thao Pham, Service de rhumatologie [CHU Cochin], Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Cochin [AP-HP], Service de Rhumatologie, Assistance publique - Hôpitaux de Paris (AP-HP)-Hôpital Ambroise Paré, CIC - CHU Bichat, Institut National de la Santé et de la Recherche Médicale ( INSERM ), Service de rhumatologie [CHU Henri Mondor], Assistance publique - Hôpitaux de Paris (AP-HP)-Hôpital Henri Mondor, Laboratoire d'Investigation Clinique ( LIC ), Université Paris-Est Créteil Val-de-Marne - Paris 12 ( UPEC UP12 ), Hôpital Lapeyronie [Montpellier] ( CHU ), Recherche Epidémiologique en Santé Périnatale et Santé des Femmes et des Enfants ( UMR_S 953 ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Université Paris-Sud - Paris 11 ( UP11 ), Aide à la Décision pour une Médecine Personnalisé - Laboratoire de Biostatistique, Epidémiologie et Recherche Clinique - EA 2415 ( AIDMP ), Université Montpellier 1 ( UM1 ) -Université de Montpellier ( UM ), Rhumatologie, Université Paris Diderot - Paris 7 ( UPD7 ), Service de radiologie, Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Cochin [AP-HP]-Université Paris Descartes - Paris 5 ( UPD5 ), Service de rhumatologie [Tours], CHU Trousseau [APHP], Laboratoire Merck Sharp & Dhome, Service de rhumatologie, Assistance Publique - Hôpitaux de Marseille ( APHM ) -Hôpital de la Conception [CHU - APHM] ( LA CONCEPTION ), Hôpital Lariboisière, Assistance publique - Hôpitaux de Paris (AP-HP)-Hôpital Lariboisière-Université Paris Diderot - Paris 7 ( UPD7 ), Optimisation continue des actions thérapeutiques par l'intégration d'informations, Université de Brest ( UBO ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Centre d'Investigation Clinique ( CIC - Brest ), CHRU Brest - Service de Rhumatologie ( CHU - BREST - Rhumato ), Centre Hospitalier Régional Universitaire de Brest ( CHRU Brest ), Immunologie et Pathologie ( EA2216 ), Université de Brest ( UBO ) -IFR148, Epilepsies de l'Enfant et Plasticité Cérébrale ( U1129 ), Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Service de Pharmacologie Clinique, Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Cochin [AP-HP]-Hôpital Saint-Vincent de Paul-Université Paris Descartes - Paris 5 ( UPD5 ), Department of Rheumatology, University Hospital Maastricht, Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ) -Hôpital Jean Minjoz, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Ambroise Paré [AP-HP], Institut National de la Santé et de la Recherche Médicale (INSERM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor, Laboratoire d'Investigation Clinique (LIC), Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Hôpital Lapeyronie [Montpellier] (CHU), Recherche Epidémiologique en Santé Périnatale et Santé des Femmes et des Enfants (UMR_S 953), Université Paris-Sud - Paris 11 (UP11)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Aide à la Décision pour une Médecine Personnalisé - Laboratoire de Biostatistique, Epidémiologie et Recherche Clinique - EA 2415 (AIDMP), Université Montpellier 1 (UM1)-Université de Montpellier (UM), Université Paris Diderot - Paris 7 (UPD7), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Merck & Co. Inc, Assistance Publique - Hôpitaux de Marseille (APHM)-Hôpital de la Conception [CHU - APHM] (LA CONCEPTION), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Lariboisière-Fernand-Widal [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7), Université de Brest (UBO)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre d'Investigation Clinique (CIC - Brest), CHRU Brest - Service de Rhumatologie (CHU - BREST - Rhumato), Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), Immunologie et Pathologie (EA2216), Université de Brest (UBO)-IFR148, Epilepsies de l'Enfant et Plasticité Cérébrale (U1129), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Saint-Vincent de Paul-Université Paris Descartes - Paris 5 (UPD5), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris-Sud - Paris 11 (UP11), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpital Ambroise Paré, Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpital Henri Mondor, Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Cochin [AP-HP]-Université Paris Descartes - Paris 5 (UPD5), Assistance Publique - Hôpitaux de Marseille (APHM)-Hôpital de la Conception [CHU - APHM] (LA CONCEPTION ), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpital Lariboisière-Université Paris Diderot - Paris 7 (UPD7), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Cochin [AP-HP]-Hôpital Saint-Vincent de Paul-Université Paris Descartes - Paris 5 (UPD5), and Centre Hospitalier Régional Universitaire [Besançon] (CHRU Besançon)-Hôpital Jean Minjoz
- Subjects
Male ,Ankylosing spondlyitis ,Settore MED/16 - REUMATOLOGIA ,MESH : Retrospective Studies ,MESH : Prospective Studies ,MESH: Comorbidity ,Comorbidity ,MESH: Magnetic Resonance Imaging ,Cohort Studies ,MESH : Back Pain ,0302 clinical medicine ,Bone Density ,Epidemiology ,MESH : Female ,Longitudinal Studies ,Prospective Studies ,MESH : Bone Density ,MESH: Longitudinal Studies ,Prospective cohort study ,MESH: Bone Density ,MESH: Cohort Studies ,Case report form ,BASDAI ,Ultrasonography ,MESH : Longitudinal Studies ,MESH : Prognosis ,MESH: Pelvic Bones ,Cohort ,MESH: Follow-Up Studies ,MESH : Spondylarthritis ,MESH : Adult ,Prognosis ,Magnetic Resonance Imaging ,3. Good health ,MESH : Comorbidity ,Female ,France ,MESH: Spine ,Cohort study ,Adult ,medicine.medical_specialty ,MESH: Spondylarthritis ,MESH : Male ,MESH : Cohort Studies ,MESH : Pelvic Bones ,MESH: Prognosis ,MESH: Social Class ,03 medical and health sciences ,Rheumatology ,MESH : Magnetic Resonance Imaging ,[ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathology ,Internal medicine ,Spondylarthritis ,Spondyloarthritis ,MESH : Social Class ,MESH : Spine ,medicine ,Humans ,Pelvic Bones ,MESH : France ,Retrospective Studies ,030203 arthritis & rheumatology ,MESH: Humans ,business.industry ,MESH : Humans ,MESH: Adult ,MESH: Retrospective Studies ,MESH : Follow-Up Studies ,Retrospective cohort study ,medicine.disease ,Spine ,MESH: Male ,MESH: Prospective Studies ,MESH: France ,Social Class ,Back Pain ,MESH: Back Pain ,Physical therapy ,business ,MESH: Female ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology ,Follow-Up Studies ,030215 immunology - Abstract
Objectives The French Society of Rheumatology has initiated a large national multicenter, longitudinal, prospective follow-up of patients presenting with early inflammatory back pain in order to set up a database to facilitate several investigations on diagnosis, prognosis, epidemiology, pathogenesis and medico-economics in the field of early inflammatory back pain and spondyloarthritis. Methods Patients were recruited if they had inflammatory back pain of more than 3 months and less than 3 years. Patients will be followed every 6 months during the first 2 years then every year during at least 5 years. Apart from information collected on a Case Report Form (demographics, disease activity, severity, co-morbidities, socio-economics, treatments, radiological and MRI evaluation of the spine and the pelvis according to the local investigators, and for some centers bone densitometry and ultrasonography of entheses), the digital X-rays and MRI of the spine and pelvis are stored using a specific software (Carestream) and the biological samples (DNA, RNA, sera, urines) are centralized at the Biological Resources Center (Bichat Hospital). Results The recruitment period of the 708 patients (mean age: 34 ± 9 years, female 54%, HLA-B27 positive: 57%) in the 25 centers was 26 months (from December 2007 to April 2010). The modified New York criteria, Amor criteria, ESSG criteria and axial ASAS criteria were fulfilled by 26%, 77%, 76% and 67% of the patients at entry, respectively. A history or current symptoms suggestive of peripheral arthritis, acute anterior uveitis and inflammatory bowel disease were observed in 21%, 9% and 4% of the patients, respectively. The disease was active (BASDAI: 45 ± 20) despite an NSAID intake in 66% of the patients. Conclusion This large cohort should facilitate the conduct of researches in different areas (clinical, medico-economics, translational) in order to improve our knowledge on the pathogenesis and natural history of axial spondyloarthritis.
- Published
- 2011
90. La cohorte DESIR : un suivi à dix ans des lombalgies inflammatoires récentes en France : méthodologie et caractéristiques initiales des 708 patients recrutés
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Bernard Combe, Christian Roux, Martin Rudwaleit, Jean-Marc Treluyer, Pascal Claudepierre, Jean-Pierre Daurès, Maxime Breban, Alain Saraux, Maria Antonietta D'Agostino, Pascal Richette, Thao Pham, Antoine Feydy, Maxime Dougados, Francis Berenbaum, Philippe Goupille, Joelle Benessiano, Daniel Wendling, Patricia Dargent-Molina, Isabelle Logeart, Véronique Leblanc, Bruno Fautrel, and Désirée van der Heijde
- Subjects
Rheumatology - Abstract
Resume Objectifs La Societe francaise de rhumatologie a mis en place une importante etude prospective, nationale, multicentrique, longitudinale, de suivi d’une cohorte de patients avec rachialgie inflammatoire recente, afin de constituer une base de donnees pour faciliter la recherche sur le diagnostic, le pronostic, l’epidemiologie, la physiopathogenie et les aspects medicoeconomique des rachialgies inflammatoires recentes et des spondylarthropathies. Methodes Les patients recrutes devaient avoir une rachialgie inflammatoire evoluant depuis plus de trois mois et moins de trois ans. Ils seront suivis tous les six mois pendant les deux premieres annees, puis chaque annee pendant au moins cinq ans. En plus des informations colligees sur un questionnaire individuel (caracteristiques demographiques, activite de la maladie, severite, comorbidites, aspects socioeconomique, traitements, radiographies standard et IRM du rachis et du pelvis par les investigateurs locaux, et pour certains centres, osteodensitometrie osseuse et echographie des entheses), les radiographies numerisees et l’IRM du rachis et du pelvis sont stockees grâce a un logiciel specifique (Carestream). Les echantillons biologiques (ADN, ARN, serum, urines) sont centralises au centre de ressources biologiques (CRB) de l’hopital Bichat. Resultats La periode de recrutement des 708 patients (moyenne d’âge : 34 ± 9 ans, femmes 54 %, HLA-B27 positif : 57 %) dans les 25 centres a ete de 26 mois (de decembre 2007 a avril 2010). Les criteres de New York modifies, les criteres d’Amor, les criteres ESSG et les criteres rachidiens de l’ASAS etaient remplis par respectivement 26 %, 77 %, 76 % et 67 % des patients a l’inclusion. Des antecedents de symptomes evocateurs d’arthrite peripherique, une uveite anterieure aigue et une maladie inflammatoire chronique de l’intestin etaient presents respectivement chez 21 %, 9 % et 4 % des patients. La maladie etait active (BASDAI : 45 ± 20 mm) malgre les AINS pris par 66 % des patients. Conclusion Cette grande etude devrait faciliter la realisation de travaux de recherche dans differents domaines (clinique, medicoeconomiques, recherche translationnelle) afin d’ameliorer notre connaissance sur la physiopathologie et l’evolution naturelle des spondylarthropathies axiales.
- Published
- 2011
91. Efficacy of rituximab in primary Sjögren's syndrome with peripheral nervous system involvement: results from the AIR registry
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Alain Cantagrel, Jean Leone, D. Launay, Pierre-Yves Hatron, B. Gombert, Christian Marcelli, P. Ravaud, M. Hamidou, Olivier Fain, Arsène Mekinian, Xavier Mariette, Claire Larroche, Maxime Breban, S. Rist, and J.-E. Gottenberg
- Subjects
Adult ,Male ,Vasculitis ,medicine.medical_specialty ,Pathology ,Immunology ,Treatment outcome ,Gastroenterology ,General Biochemistry, Genetics and Molecular Biology ,Disease activity ,Antibodies, Monoclonal, Murine-Derived ,Rheumatology ,Internal medicine ,medicine ,Humans ,Immunology and Allergy ,Registries ,Aged ,business.industry ,Peripheral Nervous System Diseases ,Middle Aged ,medicine.disease ,Sjogren's Syndrome ,Treatment Outcome ,medicine.anatomical_structure ,Cryoglobulinemia ,Multicenter study ,Antirheumatic Agents ,Peripheral nervous system ,Monoclonal ,Drug Evaluation ,Female ,Rituximab ,Sjogren s ,business ,medicine.drug - Abstract
ObjectiveTo evaluate rituximab (RTX) in primary Sjögren's syndrome (pSS) with peripheral nervous system (PNS) involvement.MethodsPatients with pSS and PNS involvement who were included in the French AIR registry were analysed.Results17 patients (age 60 years (44–78 years); 14 were female) were analysed.Neurological improvement was noted in 11 patients (65%) at 3 months. Rankin scale decreased from 3 (1–5) to 2 (1–5), 2 (1–5) and 2 (1–6) after 3, 6 and 9 months (p=0.02). European Sjögren's Syndrome Disease Activity Index decreased from 18 (10–44) to 11 (5–20), 11 (5–29) and 12 (5–30) after 3, 6 and 9 months (pRTX was effective in neurological involvement in 9/10 patients with vasculitis or cryoglobulinaemia (90%) (group 1) at 3 months and in 2/7 cases (29%) without cryoglobulinaemia and vasculitis (p=0.03). Rankin and European Sjögren's Syndrome Disease Activity Index scales decreased significantly in group 1.ConclusionRTX seems effective in cryoglobulinaemia or vasculitis-related PNS involvement in pSS.
- Published
- 2011
92. Systematic candidate gene investigations in the SPA2 locus (9q32) show an association between TNFSF8 and susceptibility to spondylarthritis
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Agathe Vigier, Elena Zinovieva, Maxime Breban, Fernando Pimentel-Santos, Dirk Elewaut, Nicolas Cagnard, Brigitte Izac, R. Said-Nahal, Kurt de Vlam, Amir Kadi, Gilles Chiocchia, and Franck Letourneur
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Adult ,Candidate gene ,Genotype ,Genetic Linkage ,Immunology ,Locus (genetics) ,Single-nucleotide polymorphism ,Polymorphism, Single Nucleotide ,Rheumatology ,Spondylarthritis ,Odds Ratio ,Humans ,Immunology and Allergy ,Medicine ,SNP ,Genetic Predisposition to Disease ,Pharmacology (medical) ,Allele ,Alleles ,Genetic Association Studies ,Genetics ,business.industry ,Haplotype ,Odds ratio ,Middle Aged ,Haplotypes ,CD30 Ligand ,business - Abstract
Objective Our group previously identified a new susceptibility region linked to spondylarthritis (SpA) on chromosome 9q31–34. Fine mapping of this SPA2 locus allowed us to refine the peak of linkage to a 1.3-Mb interval. The objective of this study was to resequence most positional candidate genes lying in that region, to identify polymorphisms, and to examine their association with SpA. Methods Variants screening was performed in 30 independent patients with SpA from families with a high linkage score to the SPA2 locus and 30 control subjects. The coding regions, intron–exon boundaries, and 5′- and 3′-flanking regions of ZNF618, A1L4R1_HUMAN (AF495724), AMBP, KIF12, ORM1, ORM2, C9ORF91, ENSESTG000000230601, and TNFSF8 were resequenced to identify polymorphisms. Selected variants were genotyped in an extended French cohort (442 patients and 268 control subjects overall). Replication was performed in a combined Belgian and Portuguese cohort (433 patients and 299 control subjects). Results Variants screening allowed us to identify 98 polymorphisms, 5 of which were selected for further studies, based on statistical significance. The rare intronic single-nucleotide polymorphism (SNP) rs3181357, located in TNFSF8, was significantly associated with SpA in the French and the replication cohorts (odds ratio [OR] 2.03, P = 0.009 and OR 2.26, P = 0.0014, respectively) and in the pooled analysis (OR 2.14, P = 0.0001). Conclusion Positional candidate gene screening in the SPA2 locus allowed us to identify and replicate an association between a rare SNP located in TNFSF8 and SpA. This new finding appears to be independent of an association with a haplotype near TNFSF15, which we recently reported.
- Published
- 2011
93. Le certolizumab pegol dans le traitement de la spondyloarthrite axiale : résultats à 4 ans de l’étude RAPID-axSpA
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Maxime Breban, D. van der Heijde, Atulya (Atul) Deodhar, Luke Peterson, Walter P. Maksymowych, Maxime Dougados, F. Van den Bosch, J. Sieper, O. Davies, J. Braun, P. J. Mease, Robert Landewé, B. Hœpken, and M. Rudwaleit
- Subjects
030203 arthritis & rheumatology ,03 medical and health sciences ,0302 clinical medicine ,Rheumatology ,business.industry ,Medicine ,030212 general & internal medicine ,business - Published
- 2016
94. Effet modulateur de la molécule ICOS dans un modèle animal de spondyloarthrite (SpA)
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Gilles Chiocchia, Maxime Breban, I. Anegon, L. Araujo-Krause, A. Jobart-Malfait, B. Cherqaoui, and Q. Jouhault
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Rheumatology - Published
- 2016
95. Tocilizumab in refractory adult Still's disease
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Thierry Schaeverbeke, Olivier Fain, Eric Toussirot, Jean-Emmanuel Kahn, Michel DeBandt, Jean-Marie Berthelot, Maïté Longy-Boursier, Club Rhumatismes et Inflammation, L. Lequen, Jean Sibilia, Jean-Jacques Dubost, Maxime Breban, Xavier Puéchal, and Aleth Perdriger
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medicine.medical_specialty ,business.industry ,Still Disease ,medicine.disease ,Chest pain ,Rash ,Surgery ,chemistry.chemical_compound ,Tocilizumab ,Rheumatology ,chemistry ,Prednisone ,Internal medicine ,medicine ,medicine.symptom ,business ,Prospective cohort study ,Adverse effect ,Rheumatism ,medicine.drug - Abstract
Objective There is an unmet need for the treatment of adult Still's disease (ASD), the pathogenesis of which may involve interleukin-6 (IL-6). We report the first series of patients with ASD treated with tocilizumab (TCZ), a humanized anti–IL-6 receptor antibody. Methods All ASD patients treated with TCZ in France between July 2006 and July 2009 after failure to all available therapies were included in this cohort study. The main outcome measures were the European League Against Rheumatism (EULAR) improvement criteria and resolution of systemic symptoms at the 3- and 6-month followup periods. Results Fourteen patients with refractory ASD were included. At the start of TCZ treatment, despite a mean prednisone dosage of 23.3 mg/day, based on a 28-joint count, mean tender joints were 10.5, mean swollen joints were 7.9, and the mean Disease Activity Score in 28 joints was 5.61. Recurrent systemic involvement, including fever and rash, was present in 7 patients. TCZ was administered at 5–8 mg/kg every 2 or 4 weeks (8 mg/kg/month, n = 9). Eleven patients successfully completed the 6-month study; 1 withdrew due to necrotizing angiodermatitis, another due to chest pain at each TCZ infusion, and a third due to systemic flare. A good EULAR response was observed in 64% of patients (9 of 14) at 3 months and EULAR remission was observed in 57% (8 of 14) at 6 months. Systemic symptoms were resolved in 86% of patients (6 of 7). Moreover, corticosteroid dose was reduced by 56%. No other severe adverse effects occurred. Conclusion TCZ is a promising new treatment for ASD.
- Published
- 2010
96. Interleukin-1 Inhibitors and Dacryoadenitis in Adult-Onset Still Disease
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Gilles Hayem, Ilaria Padovano, Pierre Romero, Paul Breillat, Maxime Breban, Félicie Costantino, and Maylis Tourte
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030203 arthritis & rheumatology ,myalgia ,medicine.diagnostic_test ,business.industry ,Dacryoadenitis ,Arthritis ,Interleukin ,Inflammation ,General Medicine ,medicine.disease ,03 medical and health sciences ,0302 clinical medicine ,Edema ,Biopsy ,Immunology ,Internal Medicine ,Medicine ,030212 general & internal medicine ,medicine.symptom ,business ,Uveitis - Published
- 2017
97. Correction to: Quantitative metagenomics reveals unique gut microbiome biomarkers in ankylosing spondylitis
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Zhijun Xie, Qian Xu, Lin Liu, Tiejuan Shao, Zhijun Zheng, Jia Zhou, Linshuang Zhang, Shunfeng Cai, Chengping Wen, Haichang Li, Yongsheng Fan, Maxime Breban, Yun Huang, Changfeng Hu, Chunyan Wu, Zhixing He, Stanislav D Ehrlich, Dawei Wang, Wendi Zhong, and Nan Qin
- Subjects
0301 basic medicine ,Genetics ,Ankylosing spondylitis ,lcsh:QH426-470 ,Computational biology ,Biology ,medicine.disease ,Gut microbiome ,Human genetics ,03 medical and health sciences ,lcsh:Genetics ,030104 developmental biology ,lcsh:Biology (General) ,Metagenomics ,medicine ,Metagenomics: An Alternative Approach to Genomics ,Genome Biology ,lcsh:QH301-705.5 - Abstract
Upon publication of the original article [1], it was noted that references 11 and 22 were miscited in the reference list.
- Published
- 2017
98. Lack of association between Tenascin-C gene and spondyloarthritis
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R. Said-Nahal, Maxime Breban, Nicolas Lebrun, Franck Letourneur, Elena Zinovieva, Gilles Chiocchia, and F.-X. Laurent
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Adult ,Male ,Candidate gene ,Genotype ,Genetic Linkage ,Locus (genetics) ,Polymorphism, Single Nucleotide ,Exon ,Rheumatology ,Genetic linkage ,Spondylarthritis ,Humans ,Medicine ,Coding region ,Genetic Predisposition to Disease ,Pharmacology (medical) ,Genetic Testing ,Genetics ,business.industry ,Tenascin ,Transmission disequilibrium test ,Case-Control Studies ,Female ,business ,Candidate Disease Gene - Abstract
Objectives We previously identified a new susceptibility region linked to SpA in 9q31-34. Tenascin-C (TNC) appears as one of the best positional and functional candidate genes lying within this SPA2 locus. The objectives of the present study were to identify TNC polymorphisms, and to examine their putative association with SpA. Methods We first performed variants screening in 20 independent SpA patients from families with high linkage score to the SPA2 locus, and three unrelated controls: TNCs coding regions (28 exons), intron-exon boundaries and 5'- and 3'-flank regions were fully re-sequenced to identify polymorphisms. Then we genotyped selected variants in 183 independent trios, and assessed their intrafamilial association with SpA by transmission disequilibrium test. Results Variants screening allowed us to identify 26 polymorphisms, 7 of which were selected for further study, in addition to an intronic polymorphism previously reported as associated with Achilles tendon injuries. In intrafamilial association test, none of the variants showed significant transmission disequilibrium. Results from analysis restricted to AS were not different from those obtained on the whole SpA group. Conclusions TNC was one of the best positional and functional candidate genes within the SPA2 locus. Nevertheless, we found no association between polymorphisms in this gene and SpA. However, we cannot exclude that variants located in intronic regions or in the vicinity of TNC, which were not tested in the present study, could be implicated in the predisposition to SpA.
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- 2008
99. Spondylarthritis in the absence of B lymphocytes
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Maxime Breban, Elli Kruithof, Paul P. Tak, Dominique Baeten, Amsterdam institute for Infection and Immunity, and Clinical Immunology and Rheumatology
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Male ,musculoskeletal diseases ,Pathology ,medicine.medical_specialty ,Spondyloarthropathy ,Immunology ,Antineoplastic Agents ,Etanercept ,Antibodies, Monoclonal, Murine-Derived ,Rheumatology ,Agammaglobulinemia ,Spondylarthritis ,medicine ,Humans ,Immunology and Allergy ,Pharmacology (medical) ,B-cell lymphoma ,Aged ,B-Lymphocytes ,Ankylosing spondylitis ,Oligoarthritis ,business.industry ,Lymphoma, Non-Hodgkin ,Antibodies, Monoclonal ,Middle Aged ,medicine.disease ,Lymphoma ,stomatognathic diseases ,Antirheumatic Agents ,Rheumatoid arthritis ,Rituximab ,business ,medicine.drug - Abstract
The highly effective treatment of rheumatoid arthritis by B cell depletion and the presence of B cells in the peripheral and axial lesions of patients with spondylarthritis (SpA) raise the question as to whether B lymphocytes could also be an appropriate therapeutic target in the latter disease. We describe 2 male HLA-B27-positive patients who had active SpA despite absence of B cells. One patient developed SpA with sacroiliitis and asymmetric oligoarthritis after having been diagnosed as having severe Bruton agammaglobulinemia. Since extensive investigations excluded an infectious origin of the SpA, this case illustrates that functional B cells and/or gamma globulins are not strictly required for SpA pathogenesis. The second patient had severe axial and peripheral SpA that was treated successfully with etanercept. After discontinuation of etanercept treatment because of non-Hodgkin's B cell lymphoma, both axial and peripheral SpA symptoms relapsed rapidly, and this exacerbation of articular disease activity was not modulated by successful B cell depletion therapy for the lymphoma. Although case reports have obvious limitations, our clinical observations provide evidence that active SpA can occur in the absence of functional mature B cells and thus emphasize the need for systematic studies of the exact role and function of B lymphocytes in this disease.
- Published
- 2008
100. Recommandations de la Société française de rhumatologie pour l'utilisation des agents anti-TNF dans la spondylarthrite ankylosante et le rhumatisme psoriasique: mise à jour 2007
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Xavier Le Loët, Bruno Fautrel, Maxime Breban, Michel De Bandt, Xavier Mariette, Emmanuelle Dernis, Philippe Goupille, Francis Guillemin, Thierry Schaeverbeke, Corinne Miceli-Richard, Bernard Combe, Jean-Francis Maillefert, Daniel Wendling, Pascal Claudepierre, Thao Pham, Alain Saraux, Charles Masson, and Philippe Ravaud
- Subjects
Gynecology ,Anti tumor necrosis factor alpha ,Clinical Practice ,medicine.medical_specialty ,Rheumatology ,Anti tnf α ,business.industry ,medicine ,Anti tnf alpha ,business - Abstract
Resume Objectif Actualiser au nom de la Societe francaise de rhumatologie les recommandations pour l'utilisation en pratique quotidienne des anti-TNF α au cours de la spondylarthrite ankylosante (SA) et du rhumatisme psoriasique (RP). Methodes La methodologie recommandee par Shekelle et al., pour la mise a jour de recommandations, a ete utilisee : determination par un groupe restreint d'experts des items a reevaluer, analyse critique de la litterature, proposition de libelles par le comite restreint, validation interne et externe des recommandations. La structure restait identique, en trois themes : indication des anti-TNF α, initiation en pratique, surveillance et adaptation therapeutique. Resultats Pour retenir l'indication d'un traitement anti-TNF α : 1) le diagnostic de SA ou de RP doit etre certain. Cette certitude s'appuiera pour la SA : sur les criteres de New York modifies ou, en leur absence, sur des atteintes caracteristiques des sacro-iliaques, du rachis ou de sites peripheriques mises en evidence sur les radiographies ou le scanner (atteinte structurale) ou l'IRM (imagerie par resonance magnetique) [atteinte inflammatoire] ; pour le RP : sur des criteres valides, tels que ceux de Moll et Wright ou CASPAR ; 2) la maladie doit etre active depuis plus d'un mois avec : a) Bath ankylosing spondylitis disease activity index (BASDAI) superieur ou egal a 4 pour les formes a predominance axiale ou superieur ou egal a trois articulations douloureuses et gonflees (NAD et NAG) ; b) jugement global de l'activite par le medecin superieur ou egal a quatre (0–10) ; 3) un echec prealable a au moins trois AINS pour les formes axiales et a un traitement de fond pour les formes peripheriques (methotrexate, salazopyrine ou leflunomide) ; 4) les contre-indications doivent etre respectees. Lors de l'initiation d'un anti-TNF α : 1) un bilan pretherapeutique doit etre realise ; 2) il n'y a pas de hierarchie des molecules anti-TNF fondee sur l'efficacite. Le choix se fera dans le cadre d'une decision partagee avec le patient, en fonction des donnees de tolerance disponibles et des caracteristiques du patient ; 3) il n'y a pas d'arguments pour recommander une association systematique d'un traitement de fond conventionnel en terme d'efficacite ; 4) le suivi des patients doit etre regulier et standardise. L'adaptation d'un anti-TNF α se fondera sur les regles suivantes : 1) l'objectif therapeutique est : pour les formes axiales : amelioration du BASDAI superieure ou egale a deux points et pour les formes peripheriques : amelioration superieure ou egale a 30 % du NAD et du NAG ; 2) en cas de non-reponse, il n'y a pas d'arguments pour recommander l'introduction d'un traitement de fond. Il est possible de modifier la frequence des perfusions ou de la posologie de l'infliximab ou de remplacer un anti-TNF par un autre ; 3) en cas d'intolerance a l'un des anti-TNF, l'utilisation d'un autre anti-TNF est possible selon la nature de l'effet indesirable observe ; 4) en cas de remission, il est recommande d'envisager une reduction, voire un arret, du traitement anti-inflammatoire associe. En cas de remission prolongee, il est possible d'envisager une reduction de la posologie de l'anti-TNF.
- Published
- 2007
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