72 results on '"Kawasaki, G."'
Search Results
52. Diffuse chronic sclerosing osteomyelitis of the mandible with synovitis, acne, pustulosis, hyperostosis, and osteitis: report of a long-term follow-up case.
- Author
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Yanamoto S, Kawasaki G, Yoshitomi I, and Mizuno A
- Subjects
- Chronic Disease, Female, Humans, Middle Aged, Radiography, Panoramic, Tomography, X-Ray Computed, Acquired Hyperostosis Syndrome diagnosis, Mandibular Diseases diagnosis, Osteomyelitis diagnosis
- Published
- 2010
- Full Text
- View/download PDF
53. Ribonucleotide reductase small subunit p53R2 promotes oral cancer invasion via the E-cadherin/beta-catenin pathway.
- Author
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Yanamoto S, Kawasaki G, Yamada S, Yoshitomi I, Yoshida H, and Mizuno A
- Subjects
- Cell Line, Tumor, Collagen, Down-Regulation, Drug Combinations, Humans, Immunoblotting, Laminin, Matrix Metalloproteinases metabolism, Mouth Neoplasms pathology, Neoplasm Invasiveness, Proteoglycans, RNA, Small Interfering, Cadherins metabolism, Mouth Neoplasms metabolism, Neoplasm Proteins metabolism, Ribonucleotide Reductases metabolism, beta Catenin metabolism
- Abstract
The p53-inducible p53R2 gene has been isolated and shown to play a crucial role in DNA repair and synthesis after DNA damage. Moreover, the expression and activity of p53R2 has been reported to be associated with the anticancer agent resistance of human cancer cells. Previously, we reported that the presence of p53R2 expression was a predictive factor for regional lymph node metastasis in oral squamous cell carcinoma; however, the mechanism of cancer metastasis by p53R2 expression is still unclear. In the present study, we analyzed the correlation of p53R2 expression with cancer invasion in vitro. Three human oral cancer cell lines (SAS, HSC-3 and Ca9-22) were cultured, and the invasive potential of these cancer cells was evaluated using Matrigel invasion assay. To investigate the effect of p53R2 on cancer invasion, the down-regulation of p53R2 was examined by small interfering RNA (siRNA). Moreover, we examined the intracellular localization of cell adhesion molecules (E-cadherin and beta-catenin) in subcellular extractions of cancer cells by immunoblotting. The proteolytic activity of matrix metalloproteinases (MMPs) was assessed by gelatin zymography. Down-regulation of p53R2 significantly enhanced the invasion potential (p<0.01), and enhanced nuclear translocation of beta-catenin with loss of total cellular E-cadherin expression in p53 mutant cancer cells, but not in p53 wild-type cancer cells. These changes in the invasion index by p53R2 siRNA transfection were not accompanied by alterations in MMP activity and expression. These results suggested that the expression of p53R2 could be associated with the invasion of cancer cells, and indicated that p53R2 might promote cancer invasion via the E-cadherin/beta-catenin pathway without the alteration of MMP activity.
- Published
- 2009
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54. Clinicopathologic significance of EpCAM expression in squamous cell carcinoma of the tongue and its possibility as a potential target for tongue cancer gene therapy.
- Author
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Yanamoto S, Kawasaki G, Yoshitomi I, Iwamoto T, Hirata K, and Mizuno A
- Subjects
- Aged, Analysis of Variance, Antigens, Neoplasm genetics, Case-Control Studies, Cell Adhesion Molecules genetics, Cell Proliferation, Epithelial Cell Adhesion Molecule, Female, Genetic Therapy methods, Humans, Immunohistochemistry, Lymphatic Metastasis, Male, Middle Aged, Neoplasm Invasiveness, Proportional Hazards Models, RNA Interference, Reverse Transcriptase Polymerase Chain Reaction, Tongue Neoplasms therapy, Translocation, Genetic, beta Catenin genetics, Antigens, Neoplasm analysis, Biomarkers, Tumor analysis, Carcinoma, Squamous Cell metabolism, Cell Adhesion Molecules analysis, Gene Expression Regulation, Neoplastic, Tongue Neoplasms metabolism
- Abstract
Epithelial adhesion molecule (EpCAM) is a transmembrane glycoprotein involved in intercellular adhesion. In particular, EpCAM appears to be overexpressed by the majority of human epithelial carcinomas, including colorectal, breast, head and neck, and hepatic carcinomas. We therefore hypothesized that EpCAM would be a good molecular target for cancer gene therapy. EpCAM protein expression in 48 primary tongue cancers and 10 normal oral mucosa was evaluated using anti-EpCAM immunohistochemistry, and correlation was examined with the clinicopathologic factors. In four human tongue cancer cell lines (SAS, HSC-2, OSC19 and OSC20), we investigated EpCAM expression by reverse transcription-polymerase chain reaction (RT-PCR). The invasive potential of cancer cells was evaluated using Matrigel invasion assay. Moreover, the effect of EpCAM inhibition was analyzed using RNA interference (RNAi). EpCAM overexpression was detected in 30 of 48 tongue cancers (62.5%), and was significantly higher in primary squamous cell carcinoma (SCC) of the tongue than in normal oral mucosa. The expression of EpCAM was significantly associated with tumor size, regional lymph node metastasis, histological differentiation and invasion pattern. Cancer cell lines with higher EpCAM expression had more invasive potential. Moreover, RNAi-mediated EpCAM reduction decreased the invasion potential and proliferation activity. These results indicated that the overexpression of EpCAM was correlated with a more aggressive phenotype of tongue cancer. Moreover, we suggested that EpCAM could be a molecular target, and that RNAi targeting EpCAM could be useful for tongue cancer gene therapy.
- Published
- 2007
- Full Text
- View/download PDF
55. Rapidly growing mass of the anterior maxillary gingiva.
- Author
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Yanamoto S, Kawasaki G, Yoshida H, Yoshitomi I, Iwamoto T, Mizuno A, and Fujita S
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- Aged, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Carcinoma, Squamous Cell drug therapy, Carcinoma, Squamous Cell radiotherapy, Carcinoma, Squamous Cell surgery, Chemotherapy, Adjuvant, Diagnosis, Differential, Gingival Neoplasms drug therapy, Gingival Neoplasms radiotherapy, Gingival Neoplasms surgery, Granulomatosis with Polyangiitis diagnosis, Humans, Male, Maxillary Sinus Neoplasms drug therapy, Maxillary Sinus Neoplasms pathology, Maxillary Sinus Neoplasms radiotherapy, Maxillary Sinus Neoplasms surgery, Mycoses diagnosis, Nose Neoplasms drug therapy, Nose Neoplasms pathology, Nose Neoplasms radiotherapy, Nose Neoplasms surgery, Palatal Neoplasms drug therapy, Palatal Neoplasms pathology, Palatal Neoplasms radiotherapy, Palatal Neoplasms surgery, Radiotherapy, Adjuvant, Tuberculosis, Oral diagnosis, Carcinoma, Squamous Cell pathology, Gingival Neoplasms pathology, Maxilla surgery
- Published
- 2007
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56. High-grade papillary cystadenocarcinoma of the sublingual gland: a case report.
- Author
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Yamada S, Matsuo T, Baba N, Rokutanda S, Kawasaki G, Mizuno A, and Fujita S
- Subjects
- Aged, Cystadenocarcinoma, Papillary secondary, Diagnosis, Differential, Follow-Up Studies, Humans, Lymphatic Metastasis pathology, Lymphoma diagnosis, Male, Neoadjuvant Therapy, Neoplasm Recurrence, Local pathology, Tomography, X-Ray Computed, Cystadenocarcinoma, Papillary diagnosis, Sublingual Gland Neoplasms diagnosis
- Published
- 2007
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57. Orotate phosphoribosyl transferase mRNA expression in oral squamous cell carcinoma and its relationship with the dihydropyrimidine dehydrogenase expression and the clinical effect of 5-fluorouracil.
- Author
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Yoshitomi I, Kawasaki G, Yanamoto S, and Mizuno A
- Subjects
- Adult, Aged, Antineoplastic Agents therapeutic use, Carcinoma, Squamous Cell drug therapy, Carcinoma, Squamous Cell pathology, Case-Control Studies, Chi-Square Distribution, Dihydrouracil Dehydrogenase (NADP) analysis, Enzyme Activation, Female, Fluorouracil therapeutic use, Gene Expression, Humans, Immunohistochemistry, In Situ Hybridization methods, Male, Middle Aged, Mouth Neoplasms drug therapy, Mouth Neoplasms pathology, Neoplasm Staging, Orotate Phosphoribosyltransferase analysis, Regression Analysis, Reverse Transcriptase Polymerase Chain Reaction, Tumor Cells, Cultured, Carcinoma, Squamous Cell enzymology, Dihydrouracil Dehydrogenase (NADP) genetics, Mouth Neoplasms enzymology, Orotate Phosphoribosyltransferase genetics, RNA, Messenger analysis
- Abstract
In this study, we investigated whether orotate phosphoribosyl transferase (OPRT) correlates with the clinicopathological features and effect of 5-fluorouracil (5-FU) in human oral carcinoma. We examined the expression of OPRT mRNA by in situ hybridization in surgical specimens of oral squamous cell carcinoma. The expression of OPRT mRNA in oral carcinoma was observed in all specimens and such expression was higher than that seen in normal control tissue specimens. There was no correlation between the expression of OPRT mRNA and clinical factors, but the expression of OPRT mRNA was significantly associated with histological differentiation. The expression of OPRT mRNA showed correlation with effect of 5-FU for oral carcinoma in either in vivo or in vitro. These results suggest that the OPRT expressions may therefore be a prognostic factor of 5-FU efficacy in patients with oral squamous cell carcinoma.
- Published
- 2006
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58. Silencing of the p53R2 gene by RNA interference inhibits growth and enhances 5-fluorouracil sensitivity of oral cancer cells.
- Author
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Yanamoto S, Iwamoto T, Kawasaki G, Yoshitomi I, Baba N, and Mizuno A
- Subjects
- Cell Line, Tumor, Cell Proliferation drug effects, Humans, Mouth Neoplasms pathology, RNA, Messenger analysis, Fluorouracil pharmacology, Gene Silencing, Genes, p53, Genetic Therapy, Mouth Neoplasms therapy, RNA Interference
- Abstract
The p53R2 gene encodes the ribonucleotide reductase (RR) small subunit 2 homologue, and is induced by several stress signals activating p53, such as DNA-damaging agents. The p53R2 gene product causes an increase in the deoxynucleotide triphosphate (dNTP) pool in the nucleus, which facilitates DNA repair and synthesis. We hypothesized that p53R2 would be a good molecular target for cancer gene therapy. In this study, three human oral cancer cell lines (SAS, HSC-4 and Ca9-22), a human breast cancer cell line MCF-7, and a normal human fibroblast cell line NHDF were tested. We silenced the expression of p53R2 with the highly specific post-transcriptional suppression of RNA interference (RNAi). We investigated p53R2 expression with the reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. The sensitivity to anticancer agents was evaluated by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The expression of p53R2 showed no association with the mutational status of p53. The cancer cell lines with higher p53R2 expression were more resistant to 5-FU. RNAi-mediated p53R2 reduction selectivity inhibited growth and enhanced chemosensitivity in cancer cell lines but not in normal fibroblasts. These results suggest that basal transcription of p53R2 could be associated with the sensitivity to anticancer agents. Moreover, we assessed the possibility that p53R2 would be a good molecular target, and report that RNAi targeting of p53R2 could be useful for oral cancer gene therapy.
- Published
- 2005
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59. Expression of p53R2, newly p53 target in oral normal epithelium, epithelial dysplasia and squamous cell carcinoma.
- Author
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Yanamoto S, Kawasaki G, Yoshitomi I, and Mizuno A
- Subjects
- Gene Expression Regulation, Neoplastic, Humans, In Situ Hybridization, Mouth Mucosa cytology, Mouth Mucosa metabolism, Mouth Mucosa pathology, Mouth Neoplasms genetics, Mouth Neoplasms radiotherapy, Neoplasms, Squamous Cell genetics, Neoplasms, Squamous Cell radiotherapy, Polymorphism, Single-Stranded Conformational, Prognosis, RNA, Messenger genetics, RNA, Messenger metabolism, Ribonucleotide Reductases genetics, Survival Analysis, Tumor Suppressor Protein p53 genetics, Cell Cycle Proteins, Epithelium metabolism, Epithelium pathology, Mouth Neoplasms metabolism, Mouth Neoplasms pathology, Neoplasms, Squamous Cell metabolism, Neoplasms, Squamous Cell pathology, Ribonucleotide Reductases metabolism, Tumor Suppressor Protein p53 metabolism
- Abstract
Recently, the p53R2 gene has been isolated and shown to play a crucial role in DNA repair after DNA damage. The p53R2 gene encodes the p53 inducible ribonucleotide reductase small subunit 2 homologue, which is part of the p53 pathway. However, the function of p53R2 in human cancer is still unclear. We investigated p53R2 mRNA expression in human oral normal epithelium, epithelial dysplasias and squamous cell carcinomas (SCCs). Surgical or biopsy-proven specimens of 10 normal epithelium, 48 epithelial dysplasias and 63 SCCs were collected in our department. Then, p53R2 was identified by in situ hybridization to visualize and localize the expression of specific mRNAs. The authors examined the p53 gene mutation by polymerase chain reaction-single strand conformation polymorphism analysis. p53, mdm2, p21(WAF1/CIP1) and Ki-67 expression was detected by immunohistochemistry. p53R2 expression was detected in none of ten normal epithelium (0%), ten of 48 dysplasias (20.8%) and 33 of 63 SCCs (52.4%). In oral SCC, the expression of p53R2 was significantly associated with tumor size, lymph node metastasis and histological differentiation (P=0.014, 0.046 and 0.022, respectively). p53R2 expression was significantly associated with p53 abnormality in epithelial dysplasia and SCC (P=0.034 and 0.009, respectively). Of 63 patients, 37 received preoperative radiochemotherapy. p53R2 mRNA expression was significantly associated with the pathologic response to radiochemotherapy (P=0.031). This study suggested that p53R2 expression could be associated with oral carcinogenesis. The presence of p53R2 mRNA expression would be a predictive factor for tumor development, tumor cell differentiation and the sensitivity to radiochemotherapy in oral SCC.
- Published
- 2003
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60. Thymidylate synthase and dihydropyrimidine dehydrogenase expression in oral squamous cell carcinoma: an immunohistochemical and clinicopathologic study.
- Author
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Kawasaki G, Yoshitomi I, Yanamoto S, and Mizuno A
- Subjects
- Adult, Aged, Aged, 80 and over, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Carcinoma, Squamous Cell drug therapy, Carcinoma, Squamous Cell pathology, Case-Control Studies, Dihydrouracil Dehydrogenase (NADP), Female, Humans, Immunoenzyme Techniques, Ki-67 Antigen analysis, Male, Middle Aged, Mouth Neoplasms drug therapy, Mouth Neoplasms pathology, Neoplasm Staging, Tegafur administration & dosage, Uracil administration & dosage, Carcinoma, Squamous Cell enzymology, Mouth Neoplasms enzymology, Oxidoreductases biosynthesis, Thymidylate Synthase biosynthesis
- Abstract
Objective: Thymidylate synthase (TS) is the target enzyme for 5-fluorouracil (5-FU), and dihydropyrimidine dehydrogenase (DPD) is the first enzyme that metabolizes 5-fluorouracil. Until now, only the enzyme activities of TS and DPD have been investigated; however, there are few reports about the immunohistochemistry of TS and DPD and none regarding oral carcinoma. The purpose of this article was to investigate the expression of TS and DPD in oral squamous cell carcinoma., Study Design: In this study, 109 oral squamous cell carcinomas were investigated for the immunohistochemical expression of TS and DPD proteins., Results: The expressions of TS in carcinoma cases was significantly higher than in controls (P <.05, t test). DPD was expressed both in carcinomas and in areas adjacent to the carcinomas. There was no correlation between the clinical factors and the TS labeling index or between the clinical factors and the DPD labeling index (DPD-LI). Pathologically, DPD-LI was significantly different in both the World Health Organization classification and Anneroth's classification. The TS labeling index was significantly correlated with the Ki-67 LI (P <.05, Pearson's correlation coefficient). Although TS showed no correlation between tegafur-uracil response and TS labeling index, there was a significant correlation between the tegafur-uracil response and DPD-LI., Conclusions: TS may reveal tumor cell proliferation, but DPD-LI may correlate with a response to anticancer drug treatment.
- Published
- 2002
- Full Text
- View/download PDF
61. p53, mdm2, and p21 expression in oral squamous cell carcinomas: relationship with clinicopathologic factors.
- Author
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Yanamoto S, Kawasaki G, Yoshitomi I, and Mizuno A
- Subjects
- Aged, Biomarkers, Tumor analysis, Carcinoma, Squamous Cell genetics, Carcinoma, Squamous Cell pathology, Cyclin-Dependent Kinase Inhibitor p21, Cyclins biosynthesis, Female, Gene Expression Regulation, Neoplastic, Humans, Immunohistochemistry, Ki-67 Antigen analysis, Male, Middle Aged, Mouth Neoplasms genetics, Mouth Neoplasms pathology, Proto-Oncogene Proteins biosynthesis, Proto-Oncogene Proteins c-mdm2, Statistics, Nonparametric, Survival Analysis, Tumor Suppressor Protein p53 biosynthesis, Carcinoma, Squamous Cell metabolism, Cell Cycle Proteins biosynthesis, Mouth Neoplasms metabolism, Neoplasm Proteins biosynthesis, Nuclear Proteins
- Abstract
Objective: The purpose of this study was to clarify the correlation of expression of cell cycle-associated gene proteins with clinicopathologic factors in oral squamous cell carcinoma (SCC)., Study Design: Formalin-fixed paraffin-embedded tissues from 69 oral SCC cases and 10 normal mucosa cases were stained by immunohistochemistry (IHC) for p53, mdm 2, and p21 proteins., Results: We found p53, mdm 2, and p21 expression in 44 of 69 (63.8%), 25 of 69 (36.2%), and 37 of 69 (53.6%) oral SCCs, respectively. Ki-67-labeling index of combined p53(+)/mdm 2(+) expression cases was significantly higher than those that lacked combined expression (P =.004). Combined p53(+)/p21(+) expression showed a significant association with lymph node metastasis (P =.019). In survival analysis, combined p53(+)/p21(+) and p53(+)/mdm 2(+)/p21(+) expression was associated with poor clinical outcome (P =.018 and.012, respectively)., Conclusion: Combined p53/mdm 2 expression was associated with tumor proliferation in oral SCC. Combined p53/p21 and p53/mdm 2/p21 expression may be a predictive factor in lymph node metastasis.
- Published
- 2002
- Full Text
- View/download PDF
62. Cathepsin expression in oral squamous cell carcinoma: relationship with clinicopathologic factors.
- Author
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Kawasaki G, Kato Y, and Mizuno A
- Subjects
- Adult, Aged, Aged, 80 and over, Analysis of Variance, Biomarkers, Tumor analysis, Biomarkers, Tumor blood, Biomarkers, Tumor genetics, Carcinoma, Squamous Cell pathology, Carcinoma, Squamous Cell secondary, Cathepsin B analysis, Cathepsin B blood, Cathepsin B genetics, Cathepsin D analysis, Cathepsin D blood, Cathepsin D genetics, Cathepsin H, Cathepsin L, Cathepsins blood, Cathepsins genetics, Cysteine Endopeptidases analysis, Cysteine Endopeptidases blood, Cysteine Endopeptidases genetics, Enzyme Precursors analysis, Enzyme Precursors blood, Enzyme Precursors genetics, Female, Gene Expression Regulation, Enzymologic genetics, Gene Expression Regulation, Neoplastic genetics, Gingival Neoplasms enzymology, Gingival Neoplasms pathology, Humans, Linear Models, Male, Middle Aged, Mouth Floor pathology, Mouth Neoplasms pathology, Neoplasm Invasiveness, Neoplasm Staging, Prognosis, Proliferating Cell Nuclear Antigen analysis, Statistics as Topic, Survival Rate, Tongue Neoplasms enzymology, Tongue Neoplasms pathology, Carcinoma, Squamous Cell enzymology, Cathepsins analysis, Mouth Neoplasms enzymology
- Abstract
Objective: Proteases are involved in the invasion and metastasis of carcinoma cells. In vivo, oral carcinoma cells easily invade the bone tissue and metastasize to the submandibular and neck lymph nodes. Cathepsin expression has been shown in some neoplastic tissues and serves as a prognostic indicator. The purpose of this study was to investigate the relationship between clinicopathohistologic grades and cathepsin expressions in oral squamous cell carcinoma and to investigate which cathepsin provides prognostic information for patients with oral carcinoma., Study Design: Immunohistochemical studies were performed on 78 carcinoma samples with monoclonal antibodies against cathepsins B, H, and L, and a polyclonal antibody against cathepsin D. Serial sections were stained by hematoxylin-eosin staining and classified by Anneroth's classification. Cathepsin B, H, L and D activities of blood serum were determined. Positive results indicative of the presence of cathepsin were investigated to determine any correlation between a particular cathepsin and histologic malignancy grades, tumor cell growth, serum cathepsin activities, and clinical factors., Results: Cathepsins B, H, L, and D were positive in every case. Although the labeling indices for cathepsins B (CB-LI), H (CH-LI), and D (CD-LI) for the cancer cases showed significant differences from those of controls, cathepsin L (CL-LI) of cancer cases showed no difference from that of controls (P <.05). A close correlation was found between CD-LI and T categories of TNM classification (P <.05), and between CD-LI and PCNA-LI (P <.05). Furthermore, a close correlation was found between CD-LI and N categories in TNM classification (P <.05). Pathologically, a close correlation was found between CB-LI or CD-LI and the pattern and/or stage of invasion (P <.05)., Conclusion: Cathepsin D and B expression were closely correlated with carcinoma invasion and progression. These proteases may be useful in determining the prognoses of patients with oral carcinoma.
- Published
- 2002
- Full Text
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63. Juvenile hyaline fibromatosis complicated with oral squamous cell carcinoma: a case report.
- Author
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Kawasaki G, Yanamoto S, Mizuno A, and Fujita S
- Subjects
- Antimetabolites, Antineoplastic administration & dosage, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Contracture pathology, Fatal Outcome, Female, Gingival Hypertrophy pathology, Humans, Malocclusion pathology, Middle Aged, Tegafur administration & dosage, Uracil administration & dosage, Carcinoma, Squamous Cell pathology, Fibroma pathology, Neoplasms, Multiple Primary pathology, Palatal Neoplasms pathology, Palate, Hard pathology, Skin Neoplasms pathology
- Abstract
A 45-year-old woman was referred because of swelling of the palate, gingival hypertrophy, and multiple cutaneous tumors. She had many cutaneous tumors, which covered most of her body, and she also displayed contractures of the major joints. Maxillary and mandibular gingival hypertrophy, malposition of the teeth, and swelling of the hard palate were the oral findings. The histopathologic features of the cutaneous and gingival tumors were consistent with hyaline fibromatosis, and the swelling of the palate proved to be a squamous cell carcinoma. The carcinoma was treated with tegafur/uracil and seemed to respond to this therapy.
- Published
- 2001
- Full Text
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64. High-affinity peptide ligands to prostate-specific antigen identified by polysome selection.
- Author
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Gersuk GM, Corey MJ, Corey E, Stray JE, Kawasaki GH, and Vessella RL
- Subjects
- Amino Acid Sequence, Binding, Competitive, Cloning, Molecular, Ligands, Molecular Sequence Data, Peptides chemical synthesis, Peptides genetics, Protein Binding, Peptides metabolism, Polyribosomes metabolism, Prostate-Specific Antigen metabolism
- Abstract
We have used the polysome-selection method to isolate peptide ligands that bind with high affinity to Prostate-Specific Antigen (PSA), an important prostate-cancer marker. Two random libraries, each encoding approximately 10(12) random peptides, were transcribed into RNA and translated in vitro. Polysomes were panned by affinity selection of the nascent peptides against immobilized PSA. Over 30% of the selected species had significant affinity for PSA; the dissociation constant of the complex formed by the best isolate with PSA was < 10(-9) M. Formation of streptavidin conjugates of selected peptides improved their affinities and, in one case, virtually eliminated non-specific binding. The polysome-selection method can be used to produce high-affinity peptide ligands of potential use in diagnostic and therapeutic procedures.
- Published
- 1997
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65. Malignant lymphoma of the mandible: report of a case.
- Author
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Kawasaki G, Nakai M, Mizuno A, Nakamura T, and Okabe H
- Subjects
- Antineoplastic Combined Chemotherapy Protocols therapeutic use, Cyclophosphamide administration & dosage, Diagnosis, Differential, Doxorubicin administration & dosage, Female, Humans, Lymphoma, Non-Hodgkin drug therapy, Lymphoma, T-Cell drug therapy, Mandibular Neoplasms drug therapy, Middle Aged, Prednisolone administration & dosage, Remission Induction, Vincristine administration & dosage, Lymphoma, Non-Hodgkin pathology, Lymphoma, T-Cell pathology, Mandibular Neoplasms pathology
- Abstract
Extranodal malignant lymphoma is relatively rare. When it occurs in the oral region, it is sometimes misdiagnosed as inflammatory disease. This report details a case of malignant lymphoma that involved the fight mandibular region.
- Published
- 1997
- Full Text
- View/download PDF
66. Induction of drug metabolizing enzymes by polychlorinated biphenyl in the parotid gland and relation to changes in vitamin A content and morphological changes.
- Author
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Kawasaki G, Mataki S, and Takano K
- Subjects
- Animals, Biphenyl Compounds toxicity, Cytochrome c Group drug effects, Enzyme Induction drug effects, Male, Mice, Microscopy, Electron, Microsomes drug effects, Microsomes enzymology, Microsomes ultrastructure, Parotid Gland cytology, Parotid Gland embryology, Vitamin A metabolism, Biphenyl Compounds pharmacology, NADPH-Ferrihemoprotein Reductase metabolism, Parotid Gland drug effects
- Abstract
The relationship between the morphological changes and vitamin A content during the development of acute toxicity induced by polychlorinated biphenyl (PCB) in mouse parotid glands was investigated. PCB was administered intraperitoneally at a single dose of 2 mg/kg. Ultrastructural studies revealed remarkable morphological changes in the rough endoplasmic reticulum, nucleus, Golgi apparatus and the secretory granules at 7 days after the administration of PCB. The activities of adenosine monophosphatase (AMPase) and alkaline phosphatase were increased 1 day after PCB administration. Then the activity of NADPH-cytochrome c reductase increased 4 days after PCB administration. Subsequently, the vitamin A content of the parotid glands significantly decreased at 7 days compared with the control. These sequential changes in enzyme activities implied that the decrease of vitamin A content in the parotid glands may be partly due to catabolism of vitamin A by increased activities of microsomal enzymes induced by PCB. In conclusion, it is suggested that PCB also induces drug metabolizing enzymes in the parotid gland cells and that the acute toxicity of PCB on this tissue may occur, at least partly, through the reduction of vitamin A not only by the secondary effect from liver impairment but also by the locally accelerated catabolism of vitamin A in the mouse parotid gland.
- Published
- 1994
- Full Text
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67. Nucleotide sequence of the triose phosphate isomerase gene of Saccharomyces cerevisiae.
- Author
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Alber T and Kawasaki G
- Subjects
- Amino Acid Sequence, Base Sequence, Triose-Phosphate Isomerase analysis, Carbohydrate Epimerases genetics, DNA, Fungal analysis, Genes, Saccharomyces cerevisiae genetics, Triose-Phosphate Isomerase genetics
- Abstract
The gene coding for the glycolytic enzyme triose phosphate isomerase (TPI1) was isolated from a yeast library in the shuttle vector pYE13. Selecting for a deletion mutant of the plasmid which enhances expression of the otherwise dormant yeast gene in E. coli facilitated the identification of the coding region. The DNA sequences of the wild type and mutant genes were determined by chemical methods. The 5' flanking region of the wild-type TPI1 resembles the analogous regions of the yeast genes coding for two other glycolytic enzymes. The sequence of the deletion mutant indicates that, upstream from -65 in the 5' flanking region, 3.3 kilobases have been lost from entirely within the yeast insert. The mutation reduces enzyme activity by tenfold in yeast, and its implications for the expression of the gene in yeast and E. coli are discussed. The amino acid sequence deduced from the nucleotide order is consistent with the electron density map of the protein as well as the sequence of its N-terminal 16 amino acids and amino acid composition. The amino acid sequence is approximately 50% homologous with the triose phosphate isomerases from rabbit, chicken, and coelacanth and 37% homologous with the Bacillus stearothermophilus enzyme. Residues which are thought to be catalytically important are conserved.
- Published
- 1982
68. Comment on antimicrobial susceptibility testing.
- Author
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Zeisler JA, Bugos DW, and Kawasaki G
- Subjects
- Drug Administration Schedule, Humans, Anti-Infective Agents administration & dosage, Microbial Sensitivity Tests
- Published
- 1983
- Full Text
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69. Solving the very big problems of the morbidly obese.
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Kawasaki G, Benz LA, Reeder L, and Provo L
- Subjects
- Equipment and Supplies, Hospital, Humans, Obesity drug therapy, Pharmaceutical Preparations administration & dosage, Obesity nursing
- Published
- 1980
- Full Text
- View/download PDF
70. Cloning of yeast glycolysis genes by complementation.
- Author
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Kawasaki G and Fraenkel DG
- Subjects
- Gene Expression Regulation, Genes, Glucose-6-Phosphate Isomerase genetics, Phosphoglycerate Kinase genetics, Phosphoglycerate Mutase genetics, Pyruvate Kinase genetics, Yeasts genetics, Cloning, Molecular methods, DNA, Glycolysis, Yeasts enzymology
- Published
- 1982
- Full Text
- View/download PDF
71. [Mercury pollution in the Jinzu River (author's transl)].
- Author
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Kawasaki G, Maeda S, Miyamoto I, Nishino H, and Arai M
- Subjects
- Animals, Fishes metabolism, Japan, Mercury analysis, Water Pollution, Chemical analysis
- Published
- 1973
- Full Text
- View/download PDF
72. [Analysis of cadmium, lead and zinc by atomic absorption spectrometry. 2. Simultaneous extraction of the heavy metals from soil].
- Author
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Kawasaki G, Senda I, Maeda S, Nishino H, and Arai M
- Subjects
- Absorption, Carbon Tetrachloride, Hydrogen-Ion Concentration, Indicators and Reagents, Japan, Cadmium analysis, Lead analysis, Soil analysis, Spectrophotometry, Zinc analysis
- Published
- 1970
- Full Text
- View/download PDF
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