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Submitted by Mario BC (mario@bc.ufrpe.br) on 2022-09-09T17:34:55Z No. of bitstreams: 1 Millena Maria Monteiro.pdf: 1011534 bytes, checksum: 49be078ce0b4b8b2e0e322c00d683060 (MD5) Made available in DSpace on 2022-09-09T17:34:55Z (GMT). No. of bitstreams: 1 Millena Maria Monteiro.pdf: 1011534 bytes, checksum: 49be078ce0b4b8b2e0e322c00d683060 (MD5) Previous issue date: 2021-12-20 This study aimed to investigate the effect of antifreeze protein type III (AFP III) added to cryopreservation extender of goat sperm on post-thawing sperm viability, with the objective of improving semen cryopreservation protocols. In the first experiment, 16 testicular pairs were collected in a slaughterhouse and transported at approximately 5 °C in a thermal box around 10 hours, until processing in the laboratory. Epididymis spermatozoa were recovered by washing and evaluated with phase contrast microscope. Then cryopreserved in Egg Yolk based extender , supplemented with antifreeze protein type III (0; 1; 10; 100 μg/mL), using automated system. After thawing (37 C /30 sec), spermatic kinetics were evaluated by CASA automated system, acrosome plasma membrane integrity, mitochondrial membrane potential and intracellular ROS production by flow cytometry. No difference (P ≥ 0.05) was seen between the experimental groups for the parameters of spermatic kinetics, mitochondrial membrane potential and ROS production. However, the integrity of plasma membrane and acrosome of frozen sperm with 100 μg/mL of AFP III was lower (P < 0.05) when compared to control group. The addition of AFP III to tris-egg yolk extender, used in the freezing of sperm obtained from the epididymis of goats, does not favor preservation of goat sperm recovered from epididymis. In addition, when at high concentration (100 μg/mL) it compromises the integrity of the plasma and acrosome membrane of these cells. The second experiment was divided into two parts: Experiment I and II, where the effect of AFP III on extenders based on Tris- egg yolk and skimmed milk was investigated, respectively. The semen of four Saanen goats (6 replicates) was used for pool formation and used in Egg Tris-yolk Diluent (Experiment I) and Skim Milk Based Diluent (Experiment II), both supplemented with AFP III concentrations, obtaining the following sample groups: 0; 1; 10; 100 μg/mL. After dilution (200 x 106 sperm/mL), the samples were filled in 0.25 mL straws and frozen (-196 ºC). For each group, two straws were thawed (37 ºC for 30 s) and grouped for in vitro analysis of spermatic kinetics, through CASA; and plasma membrane integrity (IMP) and acrosome integrity (IAC) by epifluorescence microscopy. Two straws were thawed for ultrastructural analysis of spermatozoa by scanning electron microscopy. No difference (P ≥ 0.05) was seen between experimental groups from the two experiments for the parameters of kinetics, plasma membrane integrity and acrosome. Except for the progressive motility that the concentration of 1 and 100 μg/mL differed (P < 0.05) from each other when skim milk Based Diluent was used. The results of the ultrastructural evaluation showed that regardless the diluent used, AFP III damaged the plasma membrane of the spermatic cells in a dose-dependent manner. Based on the results of the present study, it is concluded that AFP III in the concentrations used, in extenders based on egg tris-yolk and skimmed milk, does not improve seminal quality. Este estudo teve por objetivo investigar o efeito da proteína anticongelante do tipo III, adicionada ao diluidor de criopreservação de espermatozoide caprino, sobre a viabilidade espermática pós-descongelação. No primeiro experimento, 16 pares de testículos e epididimos foram obtidos em abatedouro, e transportados a, aproximadamente, 5 °C em caixa térmica em torno de 10 horas, até o recebimento e processamento do mesmo. Os espermatozoides foram recuperados e avaliados em microscópio de contraste de fase. Congelados em diluidor à base de Tris-gema de ovo, suplementado com proteína anticongelante tipo III – PAC III (0; 1; 10; 100 μg/mL), utilizando o sistema automatizado. Após descongelação (37 oC/30 seg), foram avaliadas cinética espermática, pelo sistema automatizado CASA, integridade de membrana plasmática e acrossomal, potencial de membrana mitocondrial e produção intracelular de ROS, por citometria de fluxo. Não houve diferença (P ≥ 0,05) entre os grupos experimentais para os parâmetros de cinética espermática, potencial de membrana mitocondrial e produção de ROS. A integridade de membrana plasmática e acrossomal de espermatozoides congelados com 100 μg/mL de PAC III foi inferior (P< 0,05) ao grupo controle. O segundo experimento foi dividido em duas partes, onde foi investigado o efeito da PAC III em diluidor à base de Tris-gema de ovo (Experimento I) e Leite desnatado (Experimento II). O sêmen de quatro bodes Saanen (6 repetições) foi utilizado para formação do pool e testado nos Diluentes Tris-gema de ovo (Experimento I) ou Leite desnatado (Experimento II), ambos suplementados com concentrações da PAC III (0; 1; 10; 100 μg/mL). Após a diluição (200 x 106 espermatozoides/mL), as amostras foram envasadas em palhetas (0,25 mL) e congeladas (-196 ºC). Para cada grupo, duas palhetas foram descongeladas (37 ºC por 30 s) e agrupadas para análise in vitro da cinética espermática (CASA) e da integridade de membrana plasmática e de acrossoma (microscopia de epifluorescência). Duas palhetas foram descongeladas para análise ultraestrutural dos espermatozoides por microscopia eletrônica de varredura. Não houve diferença (P ≥ 0,05) entre os grupos experimentais dos dois Experimentos para os parâmetros de cinética espermática, integridade de membrana plasmática e acrossomal. Os resultados da avaliação ultraestrutural mostraram que independente do diluidor utilizado, a PAC III danificou a membrana plasmática das células espermáticas. Baseado nos resultados do presente estudo, conclui-se que a adição da PAC III ao diluidor Tris-gema de ovo não favorece a criopreservação de espermatozoides epididimários caprinos, além disso, quando em alta concentração (100 μg/mL) compromete a integridade de membrana plasmática e acrossomal dessas células. Ainda, que a PAC III, nas concentrações utilizadas, em diluidor à base de Tris-gema de ovo ou leite desnatado, não preserva a qualidade seminal de espermatozoides ejaculados caprinos.

Submitted by (lucia.rodrigues@ufrpe.br) on 2023-02-02T19:24:06Z No. of bitstreams: 1 Antonio Matos Fraga Junior.pdf: 630876 bytes, checksum: d3b2d1c4f4b9cace9af897d0713b3d3f (MD5) Made available in DSpace on 2023-02-02T19:24:06Z (GMT). No. of bitstreams: 1 Antonio Matos Fraga Junior.pdf: 630876 bytes, checksum: d3b2d1c4f4b9cace9af897d0713b3d3f (MD5) Previous issue date: 2020-03-11 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES One of the main biotechnologies to improve reproductive rates generating greater economic return for cattle production is artificial insemination, but these indexes are still low, mainly caused by problems in the detection of estrous, late puberty, and postpartum anestrus that affect directly reproductive performance. The size of the ovulatory follicle at the time of insemination among other factors influences the conception rate and reproductive efficiency in fixed-time Artificial Insemination (FTAI) programs. The anti-Müllerian hormone (AMH) is a component of the β transforming growth factor family (TGF-β), known for its attributions in sexual differentiation in males and females, secreted by granulosa and cumulus cells, for exerting impacts on the follicular sensitivity to FSH hormone and initial recruitment of follicles. They regulate the efficiency of follicular selection and recruitment. The objective of this work was to evaluate whether there is a relationship between AMH concentrations with the conception rate in Girolando cows inseminated FTAI in blocks. A total of 327 Girolando cows (Bos taurus indicus x Bos taurus taurus) was used in the experiment. The females were submitted to the following synchronization protocols: for primiparous and multiparous females, the protocol began in D0 with the application of intravaginal progesterone device (CIDR®, 1.9 g, Zoetis) and intramuscular application (IM) 2 mg estradiol benzoate (Gonadiol®, 2 mL, Zoetis); in the D7, application (IM) of 25 mg dinoprost (Lutalyse® 5 mL, Zoetis); D9: application (IM) of 12.5 mg dinoprost (Lutalyse®, 2.5 mL, Zoetis) and 1 mg estradiol cypionate (E.C.P® 0.5 mL Zoetis). For nulliparous cows, changes in the previous protocol were in the Application D7 (IM) of only 12.5 mg dinoprost (Lutalyse®, 2.5mL) and D9 (IM): Application of 12.5 mg dinoprost (Lutalyse®, 2.5 mL) and 0.6 mg of estradiol cypionate (E.C.P® 0.3 mL). After eleven days from the beginning of the protocol (D11), the animals were randomly divided into two groups. Control Group (n:150) and Block Group (n:179). Ultrasound evaluations were initiated an hour earlier from conventional FTAI. After ultrasound evaluation, animals of the Block Group were divided into 4 blocks according to the size of the dominant follicle. Blood collections were performed on the D11 of the protocol, by the coccygeal vein, with heparinized vacuntainer tube. After collection, blood was centrifuged (900 x G for 15 minutes) for plasma removal, which was stored in freezer at -80º C until the analyses were performed. Serum AMH concentrations (ng/mL) were analyzed using bovine anti-Mullerian hormone (Webster, TX). The data were submitted to ANOVA, when significant effect was observed, tukey's test was performed at 5% significance for this, the statistical program RBio was used. The pregnancy rate of the Block Group (70.99%) was 28.71% higher than the Control Group (42.28%). The AMH assay has a measurable analytical interval of 20 ng/mL-0.312 ng/mL. Intraassay coefficients were 0,05), assim como na taxa de concepção, entretanto exerceram influência positiva (p

Submitted by Mario BC (mario@bc.ufrpe.br) on 2019-05-21T13:00:41Z No. of bitstreams: 1 Aline Saraiva de Oliveira.pdf: 952124 bytes, checksum: 62b016e88a0f2cc16e274bac13ed9f25 (MD5) Made available in DSpace on 2019-05-21T13:00:41Z (GMT). No. of bitstreams: 1 Aline Saraiva de Oliveira.pdf: 952124 bytes, checksum: 62b016e88a0f2cc16e274bac13ed9f25 (MD5) Previous issue date: 2018-03-01 Aiming to localize the Na+, K+-ATPase in ram sperm (raw, post-thaw and capacitated in vitro), and to evaluate the influience of ouabain addition on kinematic sperm parameters, two experiments were performed. Exp.1: semen samples (four rams, seven replicates) were harvested, diluted (Tris-gema, final concentration 200 x 106 sperm/mL) and frozen at -196 C°. Exp. 2: sêmen samples from four rams (five replicates) were diluted in TALPm (50 x106 sperm/mL) and aliquot for two precedures: in vitro capacitation (addition of 2,5% of estrous sheep serum - ESS) or ouabain addition (10-4 M), in order to block the Na+,K+-ATPase. Semen aliquots from both experiments were evaluated using CASA system for kinematic parameters and with aid of flow cytometry it was determined localization of Na+, K+-ATPase (Bodipy FL Ouabaína), evaluated plasmatic (IP) and acrosomal membrande (FITC-PNA) integrity, viability and stability of sperm plasma membrane (YO-PRO e M540). During Exp. 2, it was also evaluated the mitochondrial membrane potential (JC-1). For Exp. 2, all analysis were performed at 0h and 3h of incubation time (36 °C). These are the results: 1) The Na+, K+-ATPase was localized on middle piece of sperm flagellum, despite sample status (raw, post-thaw and capacitated); 2) the freezing-thawing proccess reduced (P

Submitted by Mario BC (mario@bc.ufrpe.br) on 2018-06-13T13:25:06Z No. of bitstreams: 1 Lucia Cristina Pereira Arruda.pdf: 996319 bytes, checksum: 616f336889e4a925389d4de5babc78e7 (MD5) Made available in DSpace on 2018-06-13T13:25:06Z (GMT). No. of bitstreams: 1 Lucia Cristina Pereira Arruda.pdf: 996319 bytes, checksum: 616f336889e4a925389d4de5babc78e7 (MD5) Previous issue date: 2018-02-23 Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq Ram semen was diluted (200 x 106 sperm/mL) in Tris-egg yolk (5% glycerol), added of myicetin (0.1, 10, 20, 30, 40, 100, 200, 300, 400 and 1000 nM) and frozen (-196 ° C). After thawing (37°C/30s), kinetics, iMPA, PMM, iROS, LPO, eMP and fertility in vivo (0 and 100 nM myricetin) were evaluated. The analyzes of 0, 1, 10, 100 and 1000 nM of myricetin showed: 10nM myricetin showed lower % RAP (P≤0.05), than 1000nM. Samples of control showed higher (P≤0.05) VAP than 10nM, while samples with 10, 100 and 1000nM of myicetin showed higher BCF (P≤0.05), when compared to control. Miricetin 1000nM presented higher percentage (P 0.05) for kinetics and flow cytometry (P> 0.05), there were also no interactions between treatment and time (P> 0.05). For both there was effect of the incubation time, in some evaluated parameters, the PM, VCL, VSL, VAP, LIN, ALH, percentage of cells with intact plasma and acrosomal membrane, oxidative stress and membrane stability were reduced (P 0,05) para cinética e citometria de fluxo (P>0,05), também não houve interações entre tratamento x tempo (P>0,05). Para ambos houve efeito do tempo de incubação, em alguns parâmetros avaliados a MP, VCL, VSL, VAP, LIN, ALH, porcentual de células com membrana plasmática e acrossomal intactas, estresse oxidativo e estabilidade de membrana foram reduzidos (P

Submitted by Mario BC (mario@bc.ufrpe.br) on 2018-06-14T13:12:29Z No. of bitstreams: 1 Thalles Cloves Maciel de Moura.pdf: 598623 bytes, checksum: 1334de126ab769ec1f7cbbe5e338795e (MD5) Made available in DSpace on 2018-06-14T13:12:29Z (GMT). No. of bitstreams: 1 Thalles Cloves Maciel de Moura.pdf: 598623 bytes, checksum: 1334de126ab769ec1f7cbbe5e338795e (MD5) Previous issue date: 2017-08-02 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES The objective of the study was to investigate the effects of different concentrations of sucrose on equine semen quality after thawing. Twenty-four ejaculates were obtained from 6 Mangalarga Marchador and Campolina stallions, in which each ejaculate was divided into 5 groups: control (freezing diluent without sucrose), positive control (Botucrio®®) and three groups consisting of the freezing diluent supplemented with Saccharose at concentrations of 25 mM, 50 mM and 100 mM. The semen samples were obtained with artificial vagina, evaluated and transported (5 ºC) to the laboratory. The semen samples were diluted (200x106 sperm / mL), packed in vales and subjected to freezing. Immediately after thawing (37 ° C, 30s), the semen samples were evaluated for kinetics, plasma and acrosomal membrane integrity, membrane stability and mitochondrial membrane potential. The addition of 50 and 100 mM sucrose to the freezing diluent increased (P

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-01-24T14:13:56Z No. of bitstreams: 1 2015 - Sandra Maria Morgado Ferreira.pdf: 5136516 bytes, checksum: e234c7bb0d1e27b7d6b27300db893f18 (MD5) Made available in DSpace on 2017-01-24T14:13:56Z (GMT). No. of bitstreams: 1 2015 - Sandra Maria Morgado Ferreira.pdf: 5136516 bytes, checksum: e234c7bb0d1e27b7d6b27300db893f18 (MD5) Previous issue date: 2015-09-30 This research was conducted at the Graduate Program in Biotechnology of the Northeast Biotechnology Network - (PPGB - RENORBIO), Pernambuco, at Federal Rural University of Pernambuco, focusing graduate students of agriculture and animal husbandry research area during the period 2011-2014, as well as coordinators and teachers from the same area. The main objective was to demonstrate the impact of the former students performance in Pernambuco state. In order to complete this research objective, it was adopted a methodology of data collection in documentation research and descriptive field research. The investigative tools were semi-structured interviews; survey of Lattes curriculum and records and official documents of the graduated students of the program and the biotechnology area. The quantitative analysis of the collected data was conducted through programs like Excel, Adobe InDdesign and Adobe Illustrator. The qualitative analysis was performed by categorical analysis technique where the information is grouped by categories. The study was carried out on the profile of graduated students and their contribution for the development of the Pernambuco state, as well as the perception of the coordinators and teachers about the social and economic impact of the program for the state. The results showed the relevance of the program referred to the training of qualified personnel; to increase the scientific publication with the dissemination of new knowledge to society in general. Also, it was demonstrated the social and economic benefits for the people with new techniques, processes and products generated through the results of students' surveys Esta pesquisa foi realizada no Programa de P?s-Gradua??o em Biotecnologia da Rede Nordeste de Biotecnologia (PPGB - RENORBIO), Ponto Focal Pernambuco, na Universidade Federal Rural de Pernambuco, cujo universo de estudo foram os egressos da ?rea de concentra??o em agropecu?ria, durante o per?odo 2011-2014, bem como os coordenadores e professores da mesma ?rea. O objetivo principal foi demonstrar o impacto da atua??o dos egressos do PPGB ? RENORBIO no Estado de Pernambuco. A fim de atingir seus objetivos, foi adotada, como metodologia de coleta de dados, a pesquisa documental e a pesquisa de campo descritiva, cujas ferramentas investigativas foram: entrevistas semiestruturadas; levantamento dos curr?culos Lattes e fichas de registros de matr?cula dos egressos e documentos oficiais do programa e da ?rea de biotecnologia da CAPES. A an?lise quantitativa dos dados coletados foi realizada nos programas Excel, Adobe InDdesign e Adobe Illustrator. Para a an?lise qualitativa optou-se pela utiliza??o da t?cnica da an?lise categorial, onde as informa??es s?o agrupadas por categorias. O estudo foi desenvolvido sobre o perfil dos alunos egressos e a contribui??o da atua??o dos mesmos para o desenvolvimento do Estado de Pernambuco, bem como a percep??o dos coordenadores e professores quanto ao impacto social e econ?mico do programa para o estado. Como resultado, constatou-se a relev?ncia do programa para a forma??o de pessoal qualificado; o aumento do acervo bibliogr?fico com a divulga??o de novos conhecimentos para a sociedade em geral; e os benef?cios sociais e econ?micos gerados ? popula??o com as novas t?cnicas, processos e produtos oriundos da aplica??o pr?tica dos resultados das pesquisas dos egressos

Submitted by (lucia.rodrigues@ufrpe.br) on 2016-05-31T12:03:46Z No. of bitstreams: 1 Anna Kelly de Lima Pontes Venancio.pdf: 1035454 bytes, checksum: 3e71ac64c4a92627a7ead6a5f6bc4e4a (MD5) Made available in DSpace on 2016-05-31T12:03:46Z (GMT). No. of bitstreams: 1 Anna Kelly de Lima Pontes Venancio.pdf: 1035454 bytes, checksum: 3e71ac64c4a92627a7ead6a5f6bc4e4a (MD5) Previous issue date: 2015-05-29 Aiming to evaluate the different levels of alfalfa hay on morphometric and qualitative parameters of the testicular parenchyma and serum testosterone levels in sheep (Ovisaries) of mixed breeds, 40 sheep were used in growth, with average body weight of 26 ± 1.85 kg to approximately eight months of age. The animals were subjected to 100 days confinement (with 44 days for adaptation to management and 56 days for the administration of the experimental diets). The treatments consisted of four levels of alfalfa hay: 0%, 20%, 40% and 60%, so was maintained proportion of 60% forage for all diets. At the end of the experiment, blood collection was performed for serum testosterone measurement and weighing of animals. After slaughter, the complex testicle-epididymis were removed and individually weighed, after we calculated the GSI. Histological sections were obtained (4 mm) of testicle, which were stained with hematoxylin-Phloxine and Toluidine Blue, mounted with entelan and analyzed morphologically and morphometrically in optical microscope. The body weight, scrotal circumference and IGS showed no statistical difference neither regression effect. Among the tubular parameters, height and epithelium area suffered decreasing linear effect. The volumetric ratio, the tunic, the individual volumes and the total Leydig cells as well as blood vessels suffered increasing linear regression effect. In sperm production there was also much effect regression as statistical difference, besides the regression suffered by the rounded number of spermatids per section. Thus, it was concluded that the inclusion of alfalfa a ratio above 20% is not recommended for breeding sheep because it compromises the testicular parenchyma. Objetivando-se avaliar os diferentes níveis de feno de alfafa sobre os parâmetros morfométricos e qualitativos do parênquima testicular e níveis séricos de testosterona em ovinos (ovis aries) sem raça definida, foram utilizados 40 carneiros em crescimento, com peso corporal médio inicial de 26 ± 1,85 kg e oito meses de idade aproximadamente. Os animais foram submetidos ao confinamento de 100 dias(sendo 44 dias para adaptação ao manejo e 56 dias para a administração das dietas experimentais). Os tratamentos foram compostos por quatro níveis de feno alfafa: 0%, 20%, 40% e 60%, de modo que foi mantida proporção de 60% de volumoso para todas as rações. Ao final do confinamento, foi realizada a coleta de sangue para dosagem de testosterona sérica e pesagem dos animais. Após o abate, os complexos testículo-epidídimo foram removidos e pesados individualmente, logo após foi calculado o índice gonadossomático. Foram obtidos cortes histológicos (4 μm) do testículo, os quais foram corados em Hematoxilina-Floxina e Azul de Toluidina, montados com Entelan e analisados morfologicamente e morfometricamente em microscópio Óptico.O peso corporal, biometria testicular e IGS não apresentaram diferença estatística nem efeito de regressão. Dentre os parâmetros tubulares, a altura e área de epitélio sofreram efeito linear decrescente. Na proporção volumétrica,a túnica própria, os volumes total e individual das células de Leydig,assim como os vasos sanguíneos sofreram efeito de regressão linear crescente. Na produção espermática também houve tanto efeito de regressão quanto diferença estatística, além da regressão sofrida pelo número de espermátides arredondadas por secção. Desta forma, foi possível concluir que a inclusão de alfafa numa proporção acima de 20% não é recomendada para ovinos reprodutores, pois compromete o parênquima testicular.

Submitted by (lucia.rodrigues@ufrpe.br) on 2017-02-10T13:08:46Z No. of bitstreams: 1 Robespierre Augusto Joaquim Araujo Silva.pdf: 876319 bytes, checksum: 0f2c3e9ca22ca2262229c589230deed3 (MD5) Made available in DSpace on 2017-02-10T13:08:46Z (GMT). No. of bitstreams: 1 Robespierre Augusto Joaquim Araujo Silva.pdf: 876319 bytes, checksum: 0f2c3e9ca22ca2262229c589230deed3 (MD5) Previous issue date: 2015-03-26 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES The objective of this study was to evaluate the influence of different extenders [Tris buffer (Tris), Tris-egg yolk (EY) and Tris-soybean lecithin 1% (SL1) and 2% (SL2)] and the removal of seminal plasma process in goat sperm stored at 5 ºC for 48 hours. Semen was collected four goats (two Toggenburg and two American Alpine), using an artificial vagina twice a week for four weeks. Ejaculates with greater than 60% motility were pooled and each pool seminal (n = 8) was used in a repeat. The pool was divided equally, half diluted without removal of seminal plasma (non washed semen – NW), and packaged in straws of 0.25 mL (200x106 sperm/mL). The second half of the pool was subjected to removal of the seminal plasma (washed semen - W) by double centrifugation (2200 g/10 min) then diluted and packaged as mentioned above. Then, the samples were chilled to 5 °C (90 min) and kept under refrigeration for 48 hours. Computer analysis of sperm kinetics (CASA) and the evaluation of the plasma membrane (PMi) and acrosomal (ACi) integrity and mitochondrial membrane potential (MMP) were determined within five minutes after reaching 5 °C (T0), and after 24 (T24) and 48 (T48) hours of storage. No influence extender (p>0.05) was observed for sperm kinetic variables of NW semen, however, independent extender, progressive motility (PM), linearity (LIN), straightness (STR), straightline velocity (VSL) and average path velocity (VAP) were lower (p0.05) in any of the kinetic variables SL2 group. However, LIN, STR, VSL values for Tris-EY and SL1 groups, and PM and VAP values for Tris-EY group, were lower in T48 compared to T0. It was observed also that in the T48 TM, PM, VSL and VAP variables of LS2 group, and PM variable of Tris-EY group were higher in semen washed when compared to semen non whased. The PMi was not influenced by the type of extender, but in NW semen PMi of Tris-EY and SL2 groups was lower (p 0.05) by extender, or by storage time or the removal of seminal plasma. Thus, it is concluded that the soybean lecithin can be used as a substitute for egg yolk for cooling of goat semen and which removal of the seminal plasma enhances the preservation of the caprine semen. O objetivo deste estudo foi avaliar a influência de diferentes diluidores [Tris tampão (Tris), Tris-Gema (TG) e Tris-lecitina de soja 1% (LS1) e 2% (LS2)] e do processo de remoção do plasma seminal no sêmen caprino armazenado a 5 ºC por 48 horas. O sêmen foi colhido de quatro caprinos (dois Toggenburg e dois Alpino Americano), com vagina artificial, duas vezes por semana, durante quatro semanas. Ejaculados com motilidade superior a 60% foram agrupados e cada pool seminal (n=8) foi utilizado em uma repetição. O pool foi igualmente dividido, sendo metade diluído, sem remoção do plasma seminal (sêmen não lavado – NL), e envasado em palhetas de 0,25 mL (200x106 espermatozoides/mL). A segunda metade do pool foi submetida a remoção do plasma seminal (sêmen lavado – L) por dupla centrifugação (2200 g/10min) e depois diluída e envasada como citado anteriormente. Em seguida, as amostras foram refrigeradas até 5 ºC (90 min) e mantidas sob refrigeração por 48 horas. Análise computadorizada da cinética espermática (CASA) e a avaliação da integridade de membrana plasmática (iMP) e acrossomal (iAC) e do potencial de membrana mitocondrial (PMM) foram determinadas cinco minutos após atingir 5 ºC (T0), após 24 (T24) e 48 (T48) horas de armazenamento. Nenhuma influência do diluidor (p>0,05) foi observada para as variáveis de cinética espermática do sêmen NL, porém, independente do diluidor, motilidade progressiva (MP), linearidade (LIN), retilinearidade (STR), velocidade em linha reta (VSL) e velocidade média do percurso (VAP) foram menores (p0,05) sobre quaisquer das variáveis cinéticas do grupo LS2. Entretanto, os valores de LIN, STR, VSL para os grupos Tris-Gema e LS1, e MP e VAP para o grupo Tris-Gema, foram inferiores no T48 em relação ao T0. Observou-se, ainda, que no T48 as variáveis de MT, MP, VSL e VAP do grupo LS2, e MP do grupo Tris-Gema foram superior no sêmen lavado quando comparado ao sêmen não lavado. A iMP não foi influenciada pelo tipo de diluidor, porém no sêmen NL a iMP dos grupos Tris-Gema e LS2 foi inferior (p0,05) pelo diluidor, nem pelo tempo de armazenamento ou pela remoção do plasma seminal. Assim, conclui-se que a lecitina de soja pode ser utilizada como substituto à gema de ovo para refrigeração do sêmen caprino e que a remoção do plasma seminal melhora a conservação do sêmen dessa espécie.

Submitted by (edna.saturno@ufrpe.br) on 2016-06-13T13:41:47Z No. of bitstreams: 1 Cibele Cavalcanti Souza de Melo.pdf: 1262965 bytes, checksum: a0b751f71b7ca0dbc7f0a215b3c2b60f (MD5) Made available in DSpace on 2016-06-13T13:41:47Z (GMT). No. of bitstreams: 1 Cibele Cavalcanti Souza de Melo.pdf: 1262965 bytes, checksum: a0b751f71b7ca0dbc7f0a215b3c2b60f (MD5) Previous issue date: 2015-02-27 Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq The aim was to evaluate the effect of the Aloe vera gel (Aloe barbadensis Miller), in association with the Tris base (hydroxymethyl aminomethane) or powdered coconut water (ACP- 106®) in canine semen conservation, as well as the action of this gel in the renewal process of the extender. Semen samples from five dogs, of breed Basset Hound, were used. In Experiment 1, samples were diluted in duplicate, using Tris plus 20% egg yolk (G1 and G2) or 5% Aloe vera (G3 and G4) and evaluated at 0, 48, 72 and 96 hours. After 48 hours, all samples were centrifuged for 10 minutes (400g) in a cooled centrifuge (5 °C). In the groups G1 and G3 the supernatant was removed and a new extender was added. In the other groups (G2 and G4) pellets were re-diluted in the same supernatant, without renewal. For Experiment 2, samples were divided into two equal aliquots, according to the treatments (G1: Tris + 20% egg yolk, control G2: Tris + 5% Aloe vera; G3: ACP-106® + 5% Aloe vera) and evaluated at 0, 24, 48 and 72 hours after cooling. In both experiments were performed sperm kinetics and membrane integrity analysis (iMP). In Experiment 1 results the diluent renewal did not affect on any parameter analyzed in the group using egg yolk (G1), shown when comparing the two treatments of this substance. The G2 (without renewal) did not demonstrate a significant difference when compared to G1 (renewal). However, groups with Tris-Aloe vera (G3 and G4) were lower (P

Submitted by Mario BC (mario@bc.ufrpe.br) on 2016-06-14T12:47:57Z No. of bitstreams: 1 Juliana Arandas Borba Monteiro.pdf: 831567 bytes, checksum: 0515256d8d8121fa8eba5bbaa130cfe9 (MD5) Made available in DSpace on 2016-06-14T12:47:57Z (GMT). No. of bitstreams: 1 Juliana Arandas Borba Monteiro.pdf: 831567 bytes, checksum: 0515256d8d8121fa8eba5bbaa130cfe9 (MD5) Previous issue date: 2015-02-27 The aim was to carry out a bibliographic study about the sperm and the influence of reactive oxygen species (ROS) and antioxidant therapies on ram semen cryopreservation, as wel as evaluated experimentally the effects of supplementary feeding with commercial rice oil in ram semen freezability. For the latter purpose, were used 16 crossbred sheep, with a mean weight of 35 kg and average age of 15 months, previously evaluated clinical and andrologicaly. These animals were divided into two groups according to the quality of semen, where the control group included animals with approved seminal parameters, and the treatment group for individuals with lower semen quality. The animals from treatment group were supplemented daily, by oral way, with 100 mL per day commercial rice oil (Horse Range), during 63 days. In turn, animals in the control group received 100 mL of saline solution daily, by oral way, during the same period. Semen and blood samples were obtained by artificial vagina and jugular venipuncture, respectively, at wich 21 days, where the first sample was taken immediately before treatment start (day zero D0). Semen from each breeder was subjectively evaluated and individually processed for freezing (-196 oC), while from the blood was recovered the serum, which was stored under freezing (- 4 oC). After thawing, the semen was evaluated for sperm kinematics in the computerized system (CASA), as well as for plasma membrane and acrosomal integrity in a flow cytometry. The blood serum was used for testosterone determination by electrochemiluminescence. At D0, total (MT) and progressive (MP) motility, VCL, VAP and VSL were higher (P 0.05 ) between the experimental groups with respect to serum testosterone concentration, regardless of evaluation time. Thus, it is concluded that oral administration of rice oil acts positively on the kinematics parameters and acrosomal integrity of ram semen subjected to cryopreservation, and that it not influence the plasma levels of testosterone. Neste trabalho objetivou-se avaliar os efeitos da alimentação suplementar com óleo de arroz comercial na congelabilidade do sêmen ovino, onde foram utilizados 16 ovinos mestiços, com peso de 35 kg e idade de 15 meses, em média, previamente submetidos à avaliação clínico-andrológica. Os animais foram separados em dois grupos experimentais, de acordo com a qualidade do sêmen, onde o grupo controle foi formado por animais aprovados quanto aos parâmetros seminais e o grupo tratamento por indivíduos com qualidade de sêmen inferior. Os animais do grupo tratamento foram suplementados diariamente, por via oral, com 100 mL por dia de óleo de arroz comercial (Gama Horse), durante 63 dias. Os animais do grupo controle receberam diariamente 100 mL, por via oral, de soro fisiológico durante o mesmo período. Colheitas de sêmen e sangue foram realizadas por vagina artificial e venopunção da jugular, respectivamente, a cada 21 dias, tendo sido a primeira colheita realizada imediatamente antes do início do tratamento (dia zero: D0). O sêmen de cada reprodutor foi avaliado subjetivamente e processado individualmente para a congelação (-196 oC), enquanto que da amostra de sangue foi recuperado o soro, o qual foi armazenado sob congelação (- 4 oC). Após descongelação, o sêmen foi avaliado quanto à cinética espermática no sistema computadorizado (CASA), bem como a integridade de membrana acrossomal e plasmática com fluorocromos no citômetro de fluxo. O soro sanguíneo foi utilizado para a dosagem de testosterona por eletroquimioluminescência. No D0, as motilidades total (MT) e progressiva (MP), VCL, VAP e VSL foram maiores (P0,05) entre os grupos experimentais com relação à concentração sérica de testosterona, independente do tempo de avaliação. Assim, conclui-se que a administração oral de óleo de arroz age positivamente sobre os parâmetros cinéticos e de integridade do acrossoma de espermatozoides de carneiros submetidos à criopreservação, e que não influencia os níveis séricos de testosterona.

Submitted by Mario BC (mario@bc.ufrpe.br) on 2017-05-04T12:12:28Z No. of bitstreams: 1 Pabola Santos Nascimento.pdf: 865543 bytes, checksum: 47ce2118a4d55e4ada483756d00b9ae6 (MD5) Made available in DSpace on 2017-05-04T12:12:28Z (GMT). No. of bitstreams: 1 Pabola Santos Nascimento.pdf: 865543 bytes, checksum: 47ce2118a4d55e4ada483756d00b9ae6 (MD5) Previous issue date: 2014-08-28 The use of sexed semen for in vitro embryo production (IVP) is a potentially effective means for obtaining the progeny with predetermined sex. For years, animal owners wanted a methodology that pre determine the sex of offspring for their herds. Rates of cleavage, morula and blastocyst seem to be affected not only by the damages caused by sexing, but it is believed that the bull factor, and other aspects that have not been fully elucidated, directly influencing its successful use. The aim of this study was to evaluate the influence of type of semen (sexed /conventional) and a bull factor over blastocyst rates when submitting bovine oocytes obtained from slaughterhouse to IVP, and also compare these results with analyzes of sperm kinetics. Oocytes (n = 959) were matured, fertilized with sexed and non-sexed semen from three 5/8 Girolando bulls. A straw of each type of semen was assessed with use of "computer-assisted semen analysis" (CASA) and fluorescence microscopy. Three replicates were performed during the experiment. Data were analyzed by SPSS 16.0 statistical program employing analysis of variance (ANOVA). The Student t test was used to detect differences between groups. The Chi-square test was used to analyze the results of embryo production. For all analyzes, values were considered significant (P

Submitted by (edna.saturno@ufrpe.br) on 2016-06-13T14:10:30Z No. of bitstreams: 1 Ellen Cordeiro Bento da Silva.pdf: 1569026 bytes, checksum: 6366a64f83b2fc475c8cead9d8dc65aa (MD5) Made available in DSpace on 2016-06-13T14:10:30Z (GMT). No. of bitstreams: 1 Ellen Cordeiro Bento da Silva.pdf: 1569026 bytes, checksum: 6366a64f83b2fc475c8cead9d8dc65aa (MD5) Previous issue date: 2014-02-26 Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES It was aim to evaluate the effect of different concentrations of phenolic antioxidants on freezability of goats sperm, as well as their action during centrifugation in continuous Percoll density gradients and after selected semen freezing. Six goat semen pools were used for each of the three experiments. In the first two experiments, each semen pool was processed and diluted in the skimmed milk based extender (7 % glycerol), with or without antioxidants, according to experiment and experimental group [1 Experiment: 0, 15, 25, 50, 75 or 100 μM catechin or epigallocatechin gallate (EGCG); Experiment 2: 0, 15, 25, 50, 75 or 100 μM resveratrol or quercetin]. In Experiment 3, each semen pool was centrifugated in continuous Percoll density gradients and frozen (with or without antioxidants) according to experimental groups (NS=non selected; S= selected; SC75= selected with 75 μM catechin; SE100= selected with 100 μM EGCG; SQ25=selected with 25 μM quercetin; SR25=selected with 25 μM resveratrol; SR75=selected with 75 μM resveratrol). In Experiment 1, immediately after thawing, the straight linear velocity (VSL) and average path velocity (VAP) were greater (P

Submitted by (edna.saturno@ufrpe.br) on 2016-07-25T11:36:05Z No. of bitstreams: 1 Lucia Cristina Pereira Arruda.pdf: 816795 bytes, checksum: 9fa61d0ae8d88b3e46fe1ef1a41a264d (MD5) Made available in DSpace on 2016-07-25T11:36:05Z (GMT). No. of bitstreams: 1 Lucia Cristina Pereira Arruda.pdf: 816795 bytes, checksum: 9fa61d0ae8d88b3e46fe1ef1a41a264d (MD5) Previous issue date: 2014-02-25 Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq With the aim of identify the best method to assess lipid peroxidation in goat and sheep sperm, semen samples were diluted in skimmed milk (Glycerol 7%) and Tris-egg yolk (Glycerol 5%) extenders, respectively, and frozen (-196 °C). After thawing (37°C/30s), samples were analyzed to lipid peroxidation by spectrophotometry and high performance liquid chromatography – DAD (TBARS method) and flow cytometry (C11-BODIPY581/591). It was observed that peroxidation levels obtained by spectrophotometry were significantly higher than those found by HPLC method. C11-BODIPY581/591 complemented the data obtained by TBARS (HLPC) method, helping to better understand the results and providing an overview of damaged caused to cells. In conclusion, the dosage method of TBARS by HPLC and C11-BODIPY581 are more appropriated to assess lipid peroxidation in goat and sheep sperm, and it can be used with success together or separated,. After determine the best method to assess plasma membrane lipid peroxidation of goat and sheep sperm cryopreserved with Trolox, semen samples were diluted in skimmed milk (goat) or tris-egg yolk (sheep) extenders, without antioxidants (control group) or with Trolox at 20 μM or 40 μM/mL. After thawing at 37oC for 30 seconds, samples were submitted to assessment of lipid peroxidation by high performance liquid chromatography (HPLC) and flow cytometry (C11-BODIPY581/591), as well as plasma membrane integrity, acrosomal integrity and sperm kinematics. Semen extenders and fresh semen samples were also assessed by HPLC. No significant difference (P>0.05) was observed among experimental groups of both species to sperm kinematics, plasma membrane and acrosomal integrity. Significant difference also was not observed (P>0.05) among treatment groups to lipid peroxidation reductionIn conclusion, the cryopreservation process did not trigger lipid peroxidation on goat and sheep sperm plasma membrane, independent of Trolox addiction (20 e 40 μM). Objetivando identificar o melhor método para avaliar a peroxidação lipídica em espermatozoides de caprinos e ovinos, amostras de sêmen foram diluídas em leite desnatado (Glicerol 7%) e Tris-gema de ovo (Glicerol 5%), respectivamente, e congeladas (-196 °C). Após descongelação (37 °C/30s), as amostras foram submetidas à avaliação da peroxidação lipídica por espectrofotometria e cromatografia líquida de alta performance - DAD (método de TBARS) e citometria de fluxo (C11-BODIPY581/591). Observou-se que as concentrações de malonaldeído obtidas por espectrofotometria foram significativamente maiores que as encontradas pelo método de HPLC. O C11-BODIPY581/591 complementou os dados obtidos pelo método do TBARS (HPLC), auxiliando na melhor compreensão dos resultados, evidenciando danos ocorridos às células. Concluindo-se que o método de dosagem de TBARS pelo HPLC e C11-BODIPY581/591 são os mais indicados para avaliação da peroxidação lipídica em espermatozoides de caprinos e ovinos podendo ser utilizados com sucesso juntas ou isoladamente. Determinados os melhores métodos, avaliou-se a ocorrência de peroxidação lipídica da membrana plasmática de espermatozoides caprinos e ovinos criopreservados com diluidor suplementado com Trolox, onde amostras de sêmen foram diluídas em leite desnatado (caprino) ou tris-gema de ovo (ovinos), sem antioxidante (grupo controle) ou adicionadas de Trolox nas concentrações de 20μM ou 40 μM /mL (tratamentos). Após descongelação a 37 °C/30s, as amostras foram submetidas à avaliação da peroxidação lipídica por cromatografia líquida de alta performance (HPLC-DAD) e citometria de fluxo (C11-BODIPY581/591). Analisou-se ainda a integridade de membrana plasmática, acrossomal e cinética espermática. Amostras dos diluidores e do sêmen fresco foram também avaliadas por HPLC. Não foi constatada diferença significativa (P>0,05) entre os grupos experimentais de ambas as espécies para os parâmetros de cinética espermática e integridade de membrana plasmática e acrossomal. Também não foi observada diferença significativa (P>0,05) entre os tratamentos para redução da peroxidação lipídica. Conclui-se que o processo de criopreservação não desencadeia a peroxidação lipídica da membrana plasmática de espermatozoides caprinos e ovinos independente da adição de Trolox (20 e 40 μM).

Submitted by (lucia.rodrigues@ufrpe.br) on 2017-02-08T14:39:09Z No. of bitstreams: 1 Felipe Costa Almeida.pdf: 906567 bytes, checksum: 56e2db3d9385785462dcff3582c0c339 (MD5) Made available in DSpace on 2017-02-08T14:39:09Z (GMT). No. of bitstreams: 1 Felipe Costa Almeida.pdf: 906567 bytes, checksum: 56e2db3d9385785462dcff3582c0c339 (MD5) Previous issue date: 2013-02-26 This work aimed to study fertility of sperm obtained from epididymis tail of Nelore bulls and subjected to cryopreservation in extenders with three different concentrations of glycerol, and stabilization times at 5 °C and antioxidants addition to semen extender. Epididymides were obtained from slaughterhouse up to one hour after the death of the animal, placed in individual plastic bags and transported in box at room temperature (28 °C). At the laboratory, spermatozoa were recovered from epididymis tail using flotation technique and then diluted in Tris-yolk egg. In experiment 1 three concentrations of glycerol (3%, 5% and 7%) was evaluated at different stabilization times (0h, 2h and 4h). In the second experiment was evaluated the effect of adding enzymatic antioxidants in epididymal spermatozoa cryopreservation, resulting in following experimental groups: (Control; CAT 50 and 100 U/mL, and SOD 50 and 100 U/mL) at a concentration of 60 x 106 sperm/mL, packaged in straws (0.25 mL) and frozen in automated system. In both experiments, semen samples were thawed at 37 °C/30 sec was evaluated for plasma membrane integrity (PMi), acrosomal integrity (Aci), mitochondrial membrane potential (MMP) and sperm kinematics. In Experiment 1, PMi and Aci analyzes demonstrated values lower (P 0.05) between control and treated groups. However, significant differences (P 0,05) entre o grupo controle e os grupos tratados. Todavia, diferenças significativas (P

Submitted by (lucia.rodrigues@ufrpe.br) on 2017-02-10T12:25:05Z No. of bitstreams: 1 Pedro Augusto Marinho Patriota Lima.pdf: 1417191 bytes, checksum: 7e1d18fc8ee49410d1d129b817022e3f (MD5) Made available in DSpace on 2017-02-10T12:25:05Z (GMT). No. of bitstreams: 1 Pedro Augusto Marinho Patriota Lima.pdf: 1417191 bytes, checksum: 7e1d18fc8ee49410d1d129b817022e3f (MD5) Previous issue date: 2013-02-08 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES The gossypol is a Polyphenolic yellow pigment (C30H30O8), toxic that is present in all parts of cotton plant and its derivatives. Experiments show the reproductive side effects caused by feed containing high percentage of bran or cottonseed and claim that gossypol might affect in different ways the reproductive system, causing sperm abnormalities and subsequent infertility. In this work, it was examined the effects caused by diet rich in cottonseed containing gossypol on hair sheep ram semen Santa Inês Breed. The work was performed with 22 rams, sexually mature, which were divided into 2 groups, being the animals confined in individual stocks. The gossypol group (Gg = 15), was supplemented with 500g of gossypol cottonseed and the control group (Gc = 7), supplemented with 500g of corn. The two groups were supplemented during 104 days and underwent semen collections, by the method of artificial vagina and measurements of scrotal circumference (C.E.), before (7 days), during (at intervals of 15 days) and after (10 days) the supplementation period. Non o statistical difference was seen in the mean C.E. group being treatment and control group respectively 27.07 cm and 27.08 cm. The variable observed in the semen were: color, aspect, volume, whirling, motility, concentration, vigor and sperm morphology. For color and aspect analyses were qualitative and no differences were seen in these parameters. The mean for seminal volume for animals who consumed cottonseed was 1.04 ml and for control group was 0.94 ml with no significant difference. In the variable whirling, motility, vigor, concentration and morphology there was no statistical difference and the results was respectively 4.12-81.6%-4.26-3.57 x 109/ml sptz – 6.7% and 3.91 control group – 74.77%-4.07-3.48 x 109 sptz/ml-5.97%. After the last semen collection 5 animals from gossypol group and 2 animals from control group was submitted to unilateral orchiectomy. The material was used to perform the comparative Histopathological examination in historesin, No difference in seminiferous tubule diameter was seen between control and gossypol group. There was a significant difference in seminiferous epithelium height, having a larger measurement at gossypol epithelium compared with control group. The height of seminiferous epithelium is an effective feature for sperm production assessment, however in this study there was no significant difference in sperm concentration between control and treatment group demonstrating that this difference did not affect sperm production. Two out of the three castrated animals from gossypol group were used as a fertility test exposing them to 10 fertile ewes during 90 days, resulting in 6pregnancies. The results showed that supplementation with cottonseed gossypol rich-diet did not influence sperm quality, testicular morphology, fertilization capacity and seminiferous tubules morphology. O gossipol é um pigmento polifenólico amarelo (C30H30O8), de natureza tóxica que está presente em todas as partes do algodoeiro e em seus derivados primários. Experimentos realizados mostram os efeitos colaterais na reprodução causadas por rações contendo alta porcentagem de farelo ou caroço de algodão e afirmam que o gossipol pode afetar de maneira multiforme o sistema reprodutivo, causando anormalidade nos espermatozoides e consequente infertilidade. Nesse trabalho foram analisados os efeitos causados pela dieta rica em caroço de algodão contendo gossipol no sêmen de ovinos deslanados da Raça Santa Inês. Para isso, o trabalho foi desenvolvido com 22 ovinos machos, em idade reprodutiva, os quais foram divididos em dois grupos, sendo os animais confinados em baias individuais. O grupo gossipol (Gg = 15), suplementado com 500g de caroço de algodão e o grupo controle (Gc = 7), suplementado com 500g de milho. Os dois grupos foram suplementados durante 104 dias e foram submetidos a coletas de sêmen, pelo método da vagina artificial e aferições de circunferência escrotal (C.E.), antes (7dias), durante (em intervalos de 15 dias) e depois (14 dias) do período de suplementação. Não houve diferença estatística na média de circunferência escrotal (C.E.) sendo a média do grupo tratamento e do grupo controle respectivamente 27,07 cm e 27,08 cm. As variáveis observadas no sêmen foram: coloração, aspecto, volume, turbilhonamento, motilidade, concentração, vigor e morfologia espermática. Para coloração e aspecto as análises foram qualitativas e não foram observadas diferenças nessas variáveis. A média do volume seminal para os animais que consumiram caroço de algodão foi de 1,04 ml e para os que não consumiram foi de 0,94 ml. Nas variáveis de turbilhonamento, motilidade, vigor, concentração e morfologia não houve diferença significativa sendo os valores do grupo tratamento respectivamente 4,12 – 81,6% - 4,26 – 3,57 x 109sptz /ml – 6,7% e do grupo controle 3,91 – 74,77% – 4,07 – 3,48 x 109sptz/ml – 5,97%. Após o término da última coleta de sêmen cinco animais sofreram orquiectomia unilateral, sendo dois do grupo controle e três do grupo gossipol. O material foi utilizado para realização de exame histopatológico comparativo em historesina, onde não apresentou diferença significativa entre tratamento e controle quanto ao diâmetro de túbulo seminífero, e apresentou diferença na altura do epitélio seminífero. Tendo o grupo gossipol maiores medidas de epitélio do que o grupo controle. A altura do epitélio seminífero é uma característica efetiva para a avaliação da produção espermática, entretanto nesse estudo não houve diferença significativa na concentração espermática entre grupo controle e tratamento demonstrando que essa diferença não afetou a produção espermática. Dois dos três animais castrados do grupo gossipol foram utilizados para um teste de fertilidade, onde cobriram 10 ovelhas férteis durante 90 dias, resultando em 6 prenheses positivas. Os resultados da pesquisa mostram que a suplementação com caroço de algodão não influenciou a qualidade espermática, a morfometria testicular, a capacidade fertilizante dos espermatozoides e a morfologia dos túbulos seminíferos.

Submitted by (edna.saturno@ufrpe.br) on 2016-10-14T15:47:28Z No. of bitstreams: 1 Lawrence de Oliveira Barros.pdf: 277797 bytes, checksum: 1f959a5c506b9c509d884cc0aa17f644 (MD5) Made available in DSpace on 2016-10-14T15:47:28Z (GMT). No. of bitstreams: 1 Lawrence de Oliveira Barros.pdf: 277797 bytes, checksum: 1f959a5c506b9c509d884cc0aa17f644 (MD5) Previous issue date: 2011-02-28 The cryopreservation of equine semen represents an important applied reproductive biotechnology of this species. When exposed to low temperatures, spermatozoa suffer structural modifications, occurring oxidative stress when there is imbalance between the production of reactive oxygen species (- ROS) and the amount of antioxidants present in seminal plasma. The objective of this study was to analyze the effect of the addition of different concentrations of the antioxidants glutathione peroxidase (GPx) and cysteine in equine frozen semen medium. Five Quarter Horse stallions, with proven fertility were used. Semen samples were collected with artificial vagina and diluted in Botu Crio added with antixodants: G1= Control (without antioxidants); G2= 0.5 mM of N-Acetil-Cysteine; G3= 1 mM of N-Acetil-Cysteine; G4 = 1 U of Glutathione Peroxidase (GPx) and G5= U of Glutathione Peroxidase (GPx). Semen samples were packed straws (0.5 mL; 150 x 106 concentration of spermatozoa/straw), frozen in automated method and stored in liquid Nitrogen (– 196 °C). Semen samples were thawed (37 °C per 30 seconds) and submitted to sperm analyses (kinematic, plasma membrane and acrosome integrity, and potential of mitocondrial membrane), immediately after thawing (T0) and after 60 minutes (T60). Semen samples supplemented with GPx 5 U and cysteine 0.5 mM did not show difference (P>0.05) on the percentage of sperms with high percentage of potential of mitocondrial membrane, as well as with intact acrosome and plasma membrane between T0 and T60 times. Frozen samples with cisteine 1 mM showed high (P0,05) na porcentagem de espermatozóides com alto potencial de membrana mitocondrial, assim como com acrossomas e membrana plasmática íntegras, entre os tempos T0 e T60. Amostras congeladas com cisteína 1 mM apresentaram maior (P

Submitted by (edna.saturno@ufrpe.br) on 2016-10-21T14:00:15Z No. of bitstreams: 1 Pedro Leopoldo Jeronimo Monteiro Junior.pdf: 660976 bytes, checksum: ed13725ce4ca27d354f333998ece958e (MD5) Made available in DSpace on 2016-10-21T14:00:15Z (GMT). No. of bitstreams: 1 Pedro Leopoldo Jeronimo Monteiro Junior.pdf: 660976 bytes, checksum: ed13725ce4ca27d354f333998ece958e (MD5) Previous issue date: 2011-02-18 The development of reproductive biotechnology has contributed to increase production and productivity of bovine livestock, enabling a more effective increase of production of genetically superior animal. Multiple Ovulation Embryo Transfer (MOET) enables the production of more than one calf per female per year. With the introduction of sexed semen in commercial scale, MOET increased its use, due to limiting factors of in vitro fertilization, associated with embryo cryopreservation. Despite considerable advances in the sexing process, fertility rate obtained using this type of semen is still less than that with use of conventional unsorted semen. The aim of this research was to study the effect of semen (unsorted or sorted) and Artificial Insemination (AI) time in the fertilization rate of superovulated Bos taurus cattle. Donors (n=9) were treated with intravaginal progesterone releasing device plus 3 mg estradiol benzoate at random stage of estrous cycle (Day 0). Superstimulation treatments began on Day 4, with 200 mg FSH-p, administered in decreasing doses, during four days with two applications daily, at 12 h interval. Along with the fifth and sixth FSH dose were administered 0,150 mg d-Cloprostenol. The intravaginal device was removed on the D7,5 and on D8,5 25 mg of LH were administered. The AI’s were performed according to the type of semen (unsorted or X-sorted) and AI time: G1= unsorted semen and AI 12 and 24 h after received LH (Control Group); G2= X-sorted semen and AI 12 and 24 h after received LH; G3= X-sorted semen and AI 24 and 36 h after received LH. Each cow was submitted to three superovulatory treatments (cross-over experimental design). On D15 embryos were recovered by nonsurgical method, evaluated and classified. The percentage of fertilized ova to G1, G2 and G3 groups were 60.87% (42/69), 10.00% (9/90) and 36.54% (19/52), respectively, being G1 group superior (p

Submitted by (edna.saturno@ufrpe.br) on 2016-11-03T14:12:07Z No. of bitstreams: 1 Sildivane Valcacia Silva.pdf: 2091723 bytes, checksum: a3f1920b1bf7539ac7a5652276f36b57 (MD5) Made available in DSpace on 2016-11-03T14:12:07Z (GMT). No. of bitstreams: 1 Sildivane Valcacia Silva.pdf: 2091723 bytes, checksum: a3f1920b1bf7539ac7a5652276f36b57 (MD5) Previous issue date: 2010-04-30 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES The objective of this work was to evaluate the effect of addition of antioxidants superoxide dismutase (SOD), glutathione (GSH), catalase (CAT), vitamin E (Trolox), and the association between CAT and SOD to Tris egg-yolk ram semen freezing extender. We used five Santa Inês breed rams, with history of fertility, and the ejaculates obtained by artificial vagina. The pool of semen samples were diluted in Tris egg-yolk plus glycerol 5% supplemented with antioxidants, according to the experiments and experimental groups: Exp 1 (G1= control group, G2= 25 U/mL SOD; G3= 50 U/mL SOD; G4= 100 U/mL SOD; G5= 2 mM GSH; G6= 5 mM GSH and G7= 7 mM GSH) and Exp 2 (G1= control group, G2= 30 μM Trolox, G3= 60 μM Trolox, G4= 120 μM Trolox, G5= 25 U/mL CAT, G6= 50 U/mL CAT; G7= 100 U/mL CAT, G8= 100 U/mL SOD + 25 U/mL CAT), at concentration of 240 x 106 spermatozoa/mL. Semen was stored in straws (0.25 mL), frozen using an automated system and stored in liquid nitrogen (- 196 °C). After thawing (37 ºC/30 seconds), samples were analyzed for plasma membrane integrity (iMP), acrosome (IAC) and mitochondrial membrane potential (MMP) with fluorescent probes, kinematics sperm by CASA, and ultrastructure spermatozoa by transmission electron microscopy (TEM). In Exp 1, evaluation was performed in vivo, with the semen used in embryo transfer program. In the Exp 1, significant differences (P0.05). For Exp 2, CAT 100 U/mL showed a lower percentage (P

Submitted by (edna.saturno@ufrpe.br) on 2016-10-13T12:10:00Z No. of bitstreams: 1 Janaina Azevedo Guimaraes.pdf: 619062 bytes, checksum: 70849b74d6e467dc824f80f5259e1fb2 (MD5) Made available in DSpace on 2016-10-13T12:10:00Z (GMT). No. of bitstreams: 1 Janaina Azevedo Guimaraes.pdf: 619062 bytes, checksum: 70849b74d6e467dc824f80f5259e1fb2 (MD5) Previous issue date: 2010-02-26 To evaluate the effect of supplementation with selenium and vitamin E in the diet of goats submitted to scrotal insulation (IE) on the profile of biochemical markers of systemic oxidative metabolism, we used 12 animals which were randomly divided into two groups (G1 = no supplementation G2 = supplemented with selenium and vitamin E). The supplementation started 60 days before the induction of insulation (IE). At the end of the induction period of 30 days, began to IE with placement of plastic bags in the testes for 18 days. Supplementing with Selenium and Vitamin E for the animals of G2 was maintained for 42 days after the end of IE, corresponding to the post-insulation (PIE). Blood samples were obtained by jugular venipuncture for analysis of reduced glutathione (GSH), thiobarbituric acid reactive substance (TBARS-MDA) and determining the ferric reducing ability of plasma (FRAP). There was a significant decrease in GSH during the IE and then in the PIE, there was a gradual increase of this variable. There was no statistical difference between groups. The determination of TBARS, no significant differences between treatments and times of collection. We recorded significant differences in FRAP for collection times. As observed in the lower mean 12 and 18 days of IE. There were no differences between groups. The use of supplemental dietary selenium and vitamin E did not reduce the concentration of free radicals formed in a position to scrotal insulation, however the model of induction of testicular heat stress was effective in promoting systemic oxidative stress. The FRAP method can be recommended as a biochemical marker of oxidative metabolism in goats with insulation. Para avaliar o efeito da suplementação com Selênio e Vitamina E na dieta de caprinos submetidos à insulação escrotal (IE) sobre o perfil de indicadores bioquímicos do metabolismo oxidativo sistêmico, utilizaram-se 12 animais os quais foram distribuídos aleatoriamente em dois grupos (G1= sem suplementação; G2 = suplementados com Selênio e Vitamina E). A suplementação foi iniciada 60 dias antes da indução da insulação escrotal (IE). Ao término do período de adaptação de 30 dias, iniciou-se a IE com colocação de bolsas plásticas nos testículos durante 18 dias. A suplementação alimentar com Selênio e Vitamina E para os animais do G2 foi mantida durante 42 dias após o término da IE, correspondendo à fase pós-insulação (PIE). Amostras de sangue foram obtidas por venopunção jugular para análise de glutationa reduzida (GSH), substância reativa ao ácido tiobarbitúrico (TBARS-MDA) e determinação da habilidade de redução férrica plasmática (FRAP). Verificou-se diminuição significativa da GSH no período da IE e, posteriormente, no período PIE, ocorreu um aumento gradativo desta variável. Não houve diferença estatística entre grupos. Quanto a determinação de TBARS, não houve diferenças significativas entre tratamentos e momentos de coleta. Foram registradas diferenças estatísticas do FRAP para os momentos de coleta. Sendo observadas menores médias no 12º e 18º dia da IE. Não foram observadas diferenças entre os grupos. O uso suplementar dietético de Selênio e Vitamina E não diminuiu a concentração de radicais livres formados em condição de insulação escrotal, no entanto o modelo de indução de estresse térmico testicular foi eficiente em promover estresse oxidativo sistêmico. O método FRAP pode ser recomendado como indicador bioquímico do metabolismo oxidativo em caprinos com insulação escrotal.

Submitted by (edna.saturno@ufrpe.br) on 2016-10-06T12:44:45Z No. of bitstreams: 1 Ellen Cordeiro Bento Silva.pdf: 751581 bytes, checksum: b7e4f33697071f1105ffa3e9cf312bdc (MD5) Made available in DSpace on 2016-10-06T12:44:45Z (GMT). No. of bitstreams: 1 Ellen Cordeiro Bento Silva.pdf: 751581 bytes, checksum: b7e4f33697071f1105ffa3e9cf312bdc (MD5) Previous issue date: 2010-02-26 Aiming to evaluate the effect of adding different cryoprotectants (glycerol, ethylene glycol or acetamide) and antioxidants (resveratrol and quercetin) on in vitro viability of ram thawed semen samples, four adult males, Santa Ines crossbred, were used. After collect semen using an artificial vagina, aliquots of semen were evaluated macroscopic and microscopically and the pool of semen was divided and diluted in Tris-yolk medium, according to experiments and experimental groups. For the Experiment I, which evaluated the cryoprotectants addition, the experimental groups were classified as: G1 = Tris-yolk egg + 5% glycerol, G2 = Tris-yolk egg + 3% ethylene glycol, G3 = Tris-yolk egg + 5% ethylene glycol; G4 = Tris-yolk egg + 2% acetamide, G5 = Tris-yolk egg + 7% acetamide. However, the Experiment II, which were used the antioxidant treatments, was divided in three sub experiments: Exp 1 [resveratrol (0, 5, 10, 15 and 20 μg/mL, respectively, R0, R5, R10, R15 and R20)]; Exp 2 [quercetin (0, 5, 10, 15 and 20 μg/mL, respectively, Q0, Q5, Q10, Q15 and Q20)], and Exp 3 [without antioxidant (RQ0), 10 μg/mL of resveratrol (R10), 5 μg/mL of quercetin (Q5), and 10 μg/mL of resveratrol + 5 μg/mL of quercetin (R10Q5)]. After dilution, aliquots of semen were packed in straws (0.25 mL), frozen (-196 °C) and evaluated after thawing (37 oC/30 seconds) for progressive motility (PM), vigor, plasma membrane integrity (PMi), mitochondrial membrane potential (MMP) and acrosome integrity (ACi). It was found that in Experiment I, G1 showed higher PM (P0.05) among groups on the MMP and ACi parameters. In Experiment II, there was no difference among groups on the PM, vigor, PMi and ACi. MMP had significant difference (P0,05) para PMM e iAC. No Experimento II, não se verificou diferença significativa (P>0,05) entre os grupos para os parâmetros MP, vigor, iMP e iAC. O PMM apresentou diferença significativa (P

Submitted by (edna.saturno@ufrpe.br) on 2016-08-01T16:15:27Z No. of bitstreams: 1 Anny Kaline Gomes Andrade Amorim.pdf: 1952052 bytes, checksum: 10023cd9df1426aca187a1cf4d10b2da (MD5) Made available in DSpace on 2016-08-01T16:15:27Z (GMT). No. of bitstreams: 1 Anny Kaline Gomes Andrade Amorim.pdf: 1952052 bytes, checksum: 10023cd9df1426aca187a1cf4d10b2da (MD5) Previous issue date: 2010-02-25 Aiming to evaluate the ultrasonographic appearance of testis and epididymis of sheep in pubertal and peripubertal age in order to establish normal parameters for this stage of reproductive development, 38 animals clinically healthy were used, twenty Santa Inês breed and 18 crossbred (Dorper x Santa Inês). In Santa Ines lambs, periodic evaluations of the development of weight, measurements of biometric characteristics of the testes and ultrasound examinations of the testes and epididymis were performed from 84 to 280 days of age, at intervals of 28 days. In crossbreed lambs, the same evaluations were performed from 140 to 280 days. Was used Falco 100 (Pie Medical) ultrasound scanner and linear transducer of 8.0 MHz. Scans were performed in the sagittal, transverse, frontal and oblique planes on the testis and tails of right and left epididymis of each animal, evaluating the echotexture of the testicular parenchyma, mediastinum and epididymis cauda, and measuring the thickness mediastinum and testis width. The testicular parenchyma showed homogeneous echogenicity, ranging from low to moderate, which increased in direct proportion to the age, being higher in pubertal lambs when compared to pre-pubertal. The echogenicity and thickness of the mediastinum increased with age, being classified as diffuse, moderately and highly echogenic. The tail of the epididymis showed hypoechoic appearance in relation to the testicular parenchyma. It was observed mild calcification in the testis parenchyma of five crossbreed lambs as multifocal hyperechoic images. Results of this study showed that ultrasound imaging can be used as an additional resource in the selection and evaluation morphophisiological of breeding sheep in pubertal and peripubertal age , helping to identify and monitoring changes progressive occurring in the testes and epididymis at this stage of reproductive development. Objetivando-se avaliar o aspecto ultrassonográfico de testículos e epidídimos de ovinos em idade peri-puberal e puberal, visando o estabelecimento de parâmetros de normalidade para esta fase do desenvolvimento reprodutivo, foram utilizados 38 animais clinicamente sadios, dos quais 20 eram da raça Santa Inês e 18 mestiços (Dorper x Santa Inês). Nos cordeiros Santa Inês, avaliações periódicas do desenvolvimento ponderal, mensurações das características biométricas testiculares e exames ultrassonográficos dos testículos e epidídimos foram realizados dos 84 (desmame) aos 280 dias de idade, em intervalos de 28 dias. Nos mestiços, as mesmas avaliações foram realizadas dos 140 aos 280 dias. Foi utilizado aparelho de ultrassom Falco 100 (Pie Medical) e transdutor linear de 8,0 MHz. As varreduras foram realizadas nos planos sagital, transversal, frontal e oblíquo dos testículos e caudas dos epidídimos direito e esquerdo de cada animal, avaliando-se a ecotextura do parênquima testicular, mediastino e cauda do epidídimo, e aferindo-se a espessura do mediastino e a largura testicular. O parênquima testicular apresentou ecogenicidade homogênea, variando de baixa a moderada, que aumentou em proporção direta com a idade, sendo maior nos cordeiros púberes quando comparados aos pré-púberes. A ecogenicidade e espessura do mediastino também aumentaram com a idade, sendo classificado em difuso, moderadamente e altamente ecogênico. A cauda do epidídimo apresentou aspecto hipoecóico em relação ao parênquima testicular. Observaram-se calcificações de grau leve no parênquima testicular de cinco dos cordeiros mestiços avaliados, as quais foram visibilizadas como imagens multifocais hiperecóicas. Concluiu-se que a ultrassonografia por imagem pode ser utilizada como recurso complementar na seleção e avaliação morfofisiológica de reprodutores ovinos em idade puberal e peri-puberal, contribuindo na identificação e monitoramento das mudanças progressivas que ocorrem nos testículos e estruturas relacionadas nesta fase do desenvolvimento reprodutivo.

Submitted by (edna.saturno@ufrpe.br) on 2016-10-18T17:08:25Z No. of bitstreams: 1 Maria Shirlei Barros.pdf: 885582 bytes, checksum: 12e5ba4dbe4dbc6811c0b54fef726658 (MD5) Made available in DSpace on 2016-10-18T17:08:25Z (GMT). No. of bitstreams: 1 Maria Shirlei Barros.pdf: 885582 bytes, checksum: 12e5ba4dbe4dbc6811c0b54fef726658 (MD5) Previous issue date: 2010-02-24 Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq With the objective of to evaluate mitochondrial membrane potential (MMP), kinetic, the structural and ultrastructural of goat sperm submitted to freezing in skimmed-milk and glycerol, superoxide dismutase and catalase. It was used five bucks of Boer race, submitted to semen collect by artificial vagina. Semen samples were diluted in skimm-milk plus glycerol (7%), aiming have 320x106 sperm/mL, supplemented with antioxidants according to the experimental groups: G1) extender (control), G2) extender SOD + 25 IU/mL; G3) extender + SOD 50 IU/mL; G4) extender + SOD 100 IU/mL; G5) extender + CAT 25 IU/mL; G6) extender + CAT 50 IU/mL; G7) extender + CAT 100 IU/mL and G8) extender + SOD 100 IU/mL + CAT 25 IU/mL. Then the samples were packed in straws (0.25 mL), frozen and stored in cold storage cylinder (-196 oC). After thawing (37 ºC/30 seconds), aliquots of semen frozen/thawed of each group were evaluated to PMM, kinetic (CASA), structure and ultrastructure of spermatozoa, where did not observe significant difference (P>0.05) among experimental groups in parameters PMM, kinetic, iMP and iAc. In ultrastructural evaluation, fresh sperm was morphologically preserved mainly on mitochondrial membrane. Analyzes of thawed cells showed greater quantity of damages on acrosome; however, in lower percentage on G8 cells. G7 cells showed ultrastructural damages in acrosomes of 92.31%ofspermatozoas. In contrast, great percentage of spermatozoa have plasma and acrossomal membranes intacts, mainly on G2, G4 and G8 groups. Based on the ultrastructural results, it can be recommended addition of SOD (100 U/mL) and CAT (25 U/mL) on the freezing goat semen diluents with skimmed-milk and glycerol; as well as other studies should be realized using higher concentrations than 100U/mL of SOD in this diluent to freezing goat semen, associated to in vivo evaluation of these antioxidant action. Objetivou-se com esse estudo avaliar o potencial de membrana mitocondrial (PMM), cinética, estrutura e ultraestrutura de espermatozoides caprinos, submetidos à criopreservação com diluente à base de leite desnatado e glicerol (7%), suplementado com superóxido dismutase (SOD) e catalase (CAT), em diferentes concentrações. Foram utilizados cinco reprodutores caprinos da raça Boer, submetidos à colheita de sêmen com vagina artificial. As amostras de sêmen foram diluídas em leite desnatado acrescido de glicerol (7%), de forma a apresentar 320x106 espermatozoides/mL, e suplementados com antioxidantes de acordo com os grupos experimentais: G1) diluente (Controle); G2) diluente + SOD 25 U/mL; G3) diluente + SOD 50 U/mL; G4) diluente + SOD 100 U/mL; G5) diluente + CAT 25 U/mL; G6) diluente + CAT 50 U/mL; G7) diluente + CAT 100 U/mL e G8) diluente + SOD 100 U/mL + CAT 25 U/mL. Em seguida, as amostras foram acondicionadas em palhetas (0,25 mL), congeladas e armazenadas em botijão criobiológico (-196o C). Após descongelação (37 oC/30 segundos), alíquotas de sêmen de cada grupo foram avaliadas quanto a PMM, cinética (CASA), iMP, iAc e ultraestrutura (microscopia eletrônica de transmissão). Não se constatou diferença significativa (P>0,05) entre os grupos experimentais dos parâmetros PMM, iMP, iAc e cinética espermática. Na avaliação ultraestrutural, espermatozoides in natura apresentaram-se morfologicamente preservados, principalmente na membrana mitocondrial. A análise das células pós-descongelação evidenciou maior quantidade de danos no acrossoma, todavia emmenor porcentual nas células do G8. O G7 apresentou dano ultraestrutural no acrossoma de 92,31% dos espermatozoides avaliados. Em contrapartida, grande porcentagem de espermatozóides apresentaram membrana plasmática e acrossomal intactas, principalmente nos grupos G2, G4, e G8. Com base nos resultados de ultraestrutura, é possível recomendar a adição de SOD (100U/mL) e CAT (25 U/mL) ao diluente de congelação do sêmen caprino à base de leite desnatado e glicerol; assim como outros estudos devem ser realizados utilizando concentrações maiores do que 100U/mL de SOD neste diluidor de congelação de sêmen caprino, associado à avaliação in vivo da ação deste antioxidante.

Submitted by (edna.saturno@ufrpe.br) on 2016-07-27T16:58:27Z No. of bitstreams: 1 Adriana Trindade Soares.pdf: 1620169 bytes, checksum: d8db8fd6a1234a1aaa06487952e44c4f (MD5) Made available in DSpace on 2016-07-27T16:58:27Z (GMT). No. of bitstreams: 1 Adriana Trindade Soares.pdf: 1620169 bytes, checksum: d8db8fd6a1234a1aaa06487952e44c4f (MD5) Previous issue date: 2010-02-23 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES The objetive of this study was to verify the effect of different concentrations of Trolox and Glutathione (GSH) it was used sperm from five bucks, collected by artificial vagina with six replicates. After subjective analysis(wave moving, motility, vigor and sperm concentration) of each ejaculate, a pool of samples was performed and diluted (240 x 106 sperm/mL) in skimmed-milk plus glycerol 7% with antioxidants, according to the experiment: Exp. 1) G1= Control, G2= Trolox 30μM, G3= Trolox 60μM and G4=Trolox 120μM; Exp. 2) G1= Control, G2= GSH 2mM, G3= GSH 5mM and G4= GSH 7mM. Samples were packed in straws (0.25 mL), frozen and stored in Liquid Nitrogen (-196 oC). After thawing (37 oC/30 seconds), semen aliquots of each group were submitted to iPM, iAc, MMP, kinematic by CASA and ultrastructure analysis. In the Exp. 1 and 2, it was observed that iMP, iAc, PMM and kinematics parameters showed no significant difference (P>0.05) among groups. Exp. 1, in ultrastructural analysis, acrosome, plasma membrane on the tail region and axoneme integrity did not differ (P>0.05) among groups, but they were observed in lower (P0.05) of the fresh samples. Higher percentage (P0.05)between Control and GSH (2, 5 and 7 mM/mL) groups, but they were lower (P0,05) entre os grupos. A ultraestrutura foi avaliada em amostras de sêmen in natura e pós descongelação. Nos Exps. 1 e 2, as análises de iMP, iAc, PMM e cinética espermática dos espermatozoides caprinos criopreservados não evidenciaram diferença significativa (P>0,05) entre os gruposControle e Trolox (30, 60 e 120 μM/mL). No Exp. 1, a análise ultraestrutural evidenciou que os porcentuais de acrossoma, membrana plasmática da região da cauda e axonema íntegros não diferiram (P>0,05) entre grupos (Controle e Trolox 30, 60 e 120 μM/mL), mas foram inferiores (P0,05) do sêmen in natura. Maior porcentagem (P0,05) entre os grupos Controle e GSH (2, 5 e 7mM/mL), mas foram inferiores (P

Submitted by (edna.saturno@ufrpe.br) on 2016-11-03T12:56:44Z No. of bitstreams: 1 Romulo Menna Barreto Valenca.pdf: 688661 bytes, checksum: d80c675dd68a0dfbf5ff078ecc666613 (MD5) Made available in DSpace on 2016-11-03T12:56:44Z (GMT). No. of bitstreams: 1 Romulo Menna Barreto Valenca.pdf: 688661 bytes, checksum: d80c675dd68a0dfbf5ff078ecc666613 (MD5) Previous issue date: 2009-10-05 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES The objective of this study was to determine the prevalence and identify the risk factors associated with infection by Leptospira spp. in commercial pig farms in the state of Alagoas, Brazil. To compose the study, were used 342 pigs (312 sows and 30 boars) from at seven swine herds distributed in five districts of the state of Alagoas. The serological diagnosis of infection was done by Microscopic Agglutination Test (SAM). The risk factors analysis was performed by the application of research questionnaires, consisting of objective questions relating to the designer, the general characteristics of the property, the production management, and reproductive health. Detected a prevalence of 16.10% (55/352) of pigs positive. The risk factors associated were: The not perform quarantine (p = 0.003, OR = 5.43, CI = 1.79 -16.41) and artificial insemination utilization (p = 0.023, OR = 3.38, CI = 1.18 - 9, 66). Was foundsignificant association of sows infection with the increased number of stillborn and mummified, and with the increase frequency of estrus recurrence and increased the numbers of days from weaning to service of seropositive sows. It is concluded that infection with Leptospira spp. is disseminated in commercial pig farms in the state of Alagoas, favoring the reproductive failures and decrease of zootechnical performance. The risk factors identified in this study are facilitators in the dissemination of infection and should be adjusted to control the disease in the herds studied. Objetivou-se com este estudo calcular a prevalência e identificar os fatores de risco associados à infecção por Leptospira spp. em suínos criados em granjas tecnificadas no Estado de Alagoas, Brasil. Para compor a amostra do estudo de prevalência foram utilizados 342 suínos, sendo 312 matrizes e 30 varrões, oriundos de sete granjas de ciclo completo e distribuídas em cinco municípios do Estado de Alagoas. O diagnóstico sorológico da infecção foi realizado pela técnica de Soroaglutinação Microscópica (SAM). A análise dos fatores de risco foi realizada por meio da aplicação de questionários constituídos por perguntas objetivas referentes ao criador, às características gerais da propriedade, ao manejo produtivo, reprodutivo e sanitário. Obteve-se uma prevalência de 16,10% (55/342) de suínos soropositivos. Os fatores associados foram: não realização de quarentena (p = 0,003; OR = 5,43; IC = 1,79 – 16,41) e a utilização da inseminação artificial (p = 0,023; OR = 3,38; IC = 1,18 – 9,66). Detectou-se associação entre a infecção dasmatrizes e o aumento do número de natimortos e mumificados, assim como com a maior freqüência de repetição de cio e aumento do intervalo desmama/estro nas matrizes soropositivas. Conclui-se que a infecção por Leptospira spp. encontra-se disseminada nas granjas suinícolas tecnificadas no Estado de Alagoas e contribui com a ocorrência de falhas reprodutivas e queda nos índices zootécnicos nessas propriedades. Os fatores de risco identificados nesse estudo são facilitadores na disseminação do agente e devem ser corrigidos para controlar a doença nos rebanhos estudados.

Submitted by (edna.saturno@ufrpe.br) on 2016-08-01T15:31:24Z No. of bitstreams: 1 Andre Mariano Batista.pdf: 715032 bytes, checksum: 92e93d004a4a86c672f3ad4c9e8e4af1 (MD5) Made available in DSpace on 2016-08-01T15:31:24Z (GMT). No. of bitstreams: 1 Andre Mariano Batista.pdf: 715032 bytes, checksum: 92e93d004a4a86c672f3ad4c9e8e4af1 (MD5) Previous issue date: 2009-02-12 Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq Sperm processing methods are routinely applied on the in vitro fertilization (IVF) systems for various species with the objective to remove seminal plasma/crioprotectant and to increase sperm quality. Aiming at study the sperm viability after use of the Swim-up or density gradient methods (Percoll® and CapriPure®) in frozen-thawed goat semen samples for use in assisted reproduction, six Boer goat semen samples were collected. Following, the samples were evaluated for concentration, progressive motility and morphology, diluted, packaged in straws (0.25 mL), frozen using machine and stored in liquid nitrogen. After thawing (37 oC for 30 s), sperm of all six goat were mixed-up making a pool and then were evaluated for progressive motility, membrane integrity (PI/DCF), acrossomal integrity (FITC/PNA) and mitochondrial membrane potential (JC-1). After that, the pool was split into two aliquots for the accomplishment of the sperm selection using the methods Swim-up and Percoll® (Experiment 1) and centrifugation in Percoll® and CapriPure® density gradient (Experiment 2). After use of each method of selection, the analyses was proceeded it from progressive motility, membrane integrity, acrossomal integrity and of mitochondrial integrity. Exp. 1 evidenced lower percentage of progressive motility spermatozoa (P0.05) if comparing submitted samples with the CapriPure® gradient. There were significant differences in the percentage of spermatozoa with high mitochondrial membrane potential inthe samples of semen submitted the selection using Percoll® (P0,05) ao se comparar às amostras submetidas ao gradiente CapriPure®. Houve diferença significativa nos porcentuais de espermatozóides com alto potencial de membrana mitocondrial nas amostras de sêmen submetidas à seleção utilizando os gradientes de densidade Percoll® (P0,05) foi observada entre os porcentuais de espermatozóides com alto potencial de membrana mitocondrial das amostras submetidas à seleção utilizando os gradientes de densidade Percoll® e CapriPure®. Com base nos resultados de espermatozóides móveis e com membranas intactas, é possível concluir que, para selecionar espermatozóides viáveis de amostras congeladas de sêmen caprino, deve-se usar a centrifugação em gradiente de densidade, e que o CapriPure® é uma boa alternativa ao uso doPercoll®.

Submitted by (edna.saturno@ufrpe.br) on 2016-08-11T13:50:55Z No. of bitstreams: 1 Bartira Pastor Andrade Sousa.pdf: 687607 bytes, checksum: 33d9827ebaf5d1b383b9c07059174be5 (MD5) Made available in DSpace on 2016-08-11T13:50:55Z (GMT). No. of bitstreams: 1 Bartira Pastor Andrade Sousa.pdf: 687607 bytes, checksum: 33d9827ebaf5d1b383b9c07059174be5 (MD5) Previous issue date: 2008-08-29 The aim of the present study was to assess the in vitro and in vivo viability of sperm cells following the addition of diluent in the refrigeration process of sheep semen and the fertilization of oocytes following the insemination of superovulated ewes. In Experiment I, three ejaculates from each of three Dorper breeders were used, collected with an artificial vagina during three repetitions with three-day intervals. The macroscopic and microscopic characteristics of the semen were analyzed before and after the pooling, analyzing sperm concentration, DNA integrity and acrosome integrity as well. The pooled semen was divided into five equal parts. Dilution was performed (1:3, semen:diluent) to establish the diluent groups: Equimix (DI), Laiciphos Green Ovine (DII), FR 4 (DIII), Equimix-Yolk [Equimix with 20% egg yolk (DIV)] and Tris-Yolk (DV; control). Each group was subdivided in quadruplicate, refrigerated and kept at 4 °C until the evaluations (MIP, vigor, DNA integrity and acrosome integrity), corresponding to 0, 12, 24, 36 and 48 hours. In the in vitro assessments, DI exhibited the greatest drop in MIP at 12 h in comparison to the other groups (p0.05). At 48 h, DII and DV were superior to the other groups (p0.05) between the types of semen and diluents. Among the total number of embryos, 86.4% exhibited quality Grades I and II, with the refrigerated semen of R-DI obtaining the best percentage (100%)(p0,05) entre si, enquanto que às 48 h o DII e o DV foram superiores (p0,05) entre os tipos de sêmen e de diluentes. No total dos embriões, 86,4% apresentaram qualidade de grau I e II, sendo o sêmen refrigerado do R-DI o de melhor percentual (100%) (p

Submitted by (edna.saturno@ufrpe.br) on 2016-07-28T13:59:26Z No. of bitstreams: 1 Andreia Fernandes de Souza.pdf: 1003019 bytes, checksum: ea7c3d1a2fe054ea8f10319bec70c776 (MD5) Made available in DSpace on 2016-07-28T13:59:26Z (GMT). No. of bitstreams: 1 Andreia Fernandes de Souza.pdf: 1003019 bytes, checksum: ea7c3d1a2fe054ea8f10319bec70c776 (MD5) Previous issue date: 2008-02-25 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES In Northeastern Brazil, the food lack on the low pluviometric index period is one of the factors that interfere on the goat reproduction, in spite of these animals be adapted to this region. The Aim of this study was to evaluating the effect of high (HPI) and low (LPI) pluviometric index on the proteins profile of goat semen of the American Alpine race raised on Camocim de São Félix, Pernambuco. It was realized semen collection by artificial vagina method during two years (2005, 2006). The semen samples were submitted to macroscopic (color, volume and appearance) and microscopic (progressive motility, vigor, concentration, morphology, and acrosome and DNA integrity) analyses and then divided into aliquots to realize the procedures: 1) freezing (0.5 mL), 2) protein dosages (1 mL) and 3) analysis of acrosome and DNA integrity (± 0.5 mL). The samples were packed in straws (0.25 mL), freezing in machine TK3000 (-0.25 °C/min, 25 °C to 5 °C, and - 20 °C/min of 5 °C to -120 °C) and stored in cryobiologic container (-196 C). After thawing (37 ° C, 30 seconds), the samples were evaluated to progressive motility (PM), vigor and acrosome and DNA integrity. The semen protein profile was conducted through the analysis of two-dimensional electrophoresis, after determination of total protein for standardization of the protein amount applied in gel electrophoresis. On the in natura semen, there was no significant difference (P>0.05) on the PM among animals and between LPI (79.82%) and HPI (81.25%) periods, as well as on sperm vigor on the LPI (4.17) and HPI (4.00) periods. However, it was observed higher(P0.05) on the cells with intact acrosome on the LPI (67.98%) and HPI (66.83%). In the protein samples extracted of in nature and thaw sperm membrane, 56 and 165 spots of proteins were identified with 25 to 103.0 kDa and 9.0 to 131.0 kDa, and with 3.36 to 9.74 and 3.37 to 8.80 pIs, respectively. On the evaluation of the seminal plasma proteins were detected 96 spots of protein, with 49 spots in the LPI period (15 to 97 kDa, 4.48 to 9.83 pI) and 47 spots in the HPI period (4 to 106 kDa and 3.00 to 8.96 pI). The periods HPI and LPI had 22 common spots of proteins, corresponding to 45.83% of similarity among spots analyzed. In the gel analysis of the thawed semen samples it was observed increase of protein spots number than in natura semen. It is suggested that groups of proteins of low molecular mass of seminal plasma membrane and the sperm of goats are responsible for sperm motility and the presence of a group of proteins on 14-15 kDa and 45kDa are responsible for the viability of the sperm cells in this experiment. Na região Nordeste do Brasil, a ausência de alimentos no período de baixo índice pluviométrico é um dos fatores que mais interferem na reprodução dos caprinos, apesar destes animais estarem bem adaptados a esta região. Objetivando avaliar o efeito dos períodos de alto (AIP) e baixo (BIP) índice pluviométrico no perfil de proteínas do sêmen de caprinos da raça Alpina Americana criados no Município de Camocim de São Felix (Pernambuco), foram realizadas colheitas de sêmen pelo método de vagina artificial em dois anos consecutivos (2005 e 2006). As amostras de sêmen foram submetidas à análise macroscópica (cor, volume e aspecto) e microscópica (motilidade progressiva, vigor, concentração, morfologia, integridade de acrossoma e DNA) e, a seguir, divididas em alíquotas com a finalidade de realizar os procedimentos de: 1) congelação (0,5 mL), 2) dosagem de proteínas (1 mL) e 3) análise de integridade de acrossoma e de DNA (± 0,5mL). As amostras foram envasadas em palhetas (0,25 mL), criopreservadas em máquina TK3000 (- 0,25 ºC/min, de 25 ºC a 5 ºC, e a – 20 ºC/ min, de 5 ºC a -120 ºC) e armazenadas em botijão criobiológico (-196 oC). Após descongelação (37 oC; 30 segundos), as amostras foram avaliadas quanto a motilidade progressiva (MP), vigor, integridade de acrossoma e de DNA. O perfil de proteínas das membranas espermáticas e do plasma seminal foi realizado através da análise de eletroforese bidimensional, após dosagem de proteína total para padronização da quantidade de proteínas aplicadas no gel de eletroforese. No sêmen in natura, não seobservou diferença significativa (P>0,05) na motilidade progressiva (MP) entre animais e entre os períodos BIP (79,82%) e AI (81,25%), assim como no vigor espermático nos períodos BIP (4,17) e AIP (4,00). Porém foi observado maior (P0,05) no porcentual de células com acrossomas íntegros no BIP (67,98%) e AIP (66,83%). Nas amostras de proteínas extraídas da membrana de espermatozóides in natura e descongelado, foram identificados 56 e 165 spots de proteínas com 25 a 103,0 kDa e 9,0 a 131,0 kDa, e com pIs 3,36 a 9,74 e de 3,37 a 8,80, respectivamente. Na avaliação das proteínas do plasma seminal foram detectados 96 spots de proteínas, sendo 49 spots no período BIP (15 a 97 kDa e pI de 4,48 a 9,83) e 47 spots no período AIP (4 a 106 kDa e pI de 3,00 a 8,96). Os períodos AIP e BIP apresentaram 22 spots de proteínas comuns, correspondendo a 45,83% de similaridade entre os spots analisados. Na análise dos géis das amostras de sêmen descongelado, constatou-se aumento do número de spots de proteínas focalizados. Sugere-se que os grupos de proteínas de baixa massa molecular do plasma seminal e da membrana espermática de caprinos interferem na motilidade espermática; e que um grupo de proteínas de 14-15 kDa e 45kDa atua na viabilidade das células espermáticas.

Submitted by (edna.saturno@ufrpe.br) on 2016-10-21T13:36:14Z No. of bitstreams: 1 Paulo Cesar Vasco de Albuquerque Peixoto.pdf: 344249 bytes, checksum: dd3eefd193ab58cfcdb099090c32ef23 (MD5) Made available in DSpace on 2016-10-21T13:36:14Z (GMT). No. of bitstreams: 1 Paulo Cesar Vasco de Albuquerque Peixoto.pdf: 344249 bytes, checksum: dd3eefd193ab58cfcdb099090c32ef23 (MD5) Previous issue date: 2008-02-15 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES In order to evaluate the effect of adding antioxidants Trolox and Glutathione reduced on the in vitro viability of dog cryopreserved sperm, semen samples were harvested from animals of the breeds French Bulldog (n=1), Basset Hound (n=2) e Rottweiler (n=1), through digital manipulation. Then proceeded to the evaluation macroscopic (color, appearance, volume) and microscopic (progressive motility, vigor, DNA, acrosome integrity and oxidative stress), followed by dividing the volume of semen on four aliquots, according to the experiments and experimental groups: Experimental 1 - G1 = Tris- Egg yolk (control group); G2 = Tris- Egg yolk + 200 uM of Trolox and G3 = Tris- Egg yolk + 300 uM of Trolox; Experimental 2 - G1 = Tris- Egg yolk (Control group); G2 = Tris- Egg yolk + 2 uM of GSH and G3 = Tris-Egg yolk + 5 uM of GSH; Experimental 3 - G1 = Tris- Egg yolk (control group); G2 = Tris- Egg yolk + 200 uM of Trolox; G3 = Tris- Egg yolk + 2 uM of GSH and G4 = Tris- Egg yolk + 200 uM of Trolox + 2 uM of GSH. The samples were packaged in straws (0.25 mL), cryopreserved in freezing machine (TK3000) and stored in liquid nitrogen (-196 oC). After thawing a 37 oC and incubation during 60 minutes, it was detected that MP, vigor and sperm with intact acrosomes had significant difference (P

Submitted by (edna.saturno@ufrpe.br) on 2016-11-07T15:17:13Z No. of bitstreams: 1 Thiago Augusto Pereira Moraes.pdf: 1403494 bytes, checksum: 178fa823d17d452549f791d6c6584857 (MD5) Made available in DSpace on 2016-11-07T15:17:13Z (GMT). No. of bitstreams: 1 Thiago Augusto Pereira Moraes.pdf: 1403494 bytes, checksum: 178fa823d17d452549f791d6c6584857 (MD5) Previous issue date: 2007-02-28 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES It was objectified to use increasing doses of pentoxifylline on the critical period of the testicular development of Wistar rats, aiming to keep high levels of AMPc in the Sertoli cells and to increase the intrinsic income of spermatogenesis and sperm production in adult rats. There were used 37 neonate Wistar rats, which were submitted to many treatments during first 21 days of life, according to experimental groups: control (n=10), 1mg/kg (n=10), 5mg/kg (n=9) and 10mg/kg (n=8) of pentoxifylline. The animal weight was obtained daily, in this period, on the control and treated groups. On the second experiment, it was used 39 adult Wistar rats, which were submitted to many treatments, between 90 and 150 days of life. in accordance with the experimental group: control (n=9), 15mg/kg (n=10), 30mg/kg (n=10) and 60mg/kg (n=10) of pentoxifylline. The animal weight was carried through daily, in this period, on the control and treated groups. At the 90 days (Exp. 1) and 150 days (Exp. 2) of the experimental period, the rats of each group were submitted to anesthesia and intracardiac perfusion. Later, the testis, epididimes and seminal vesicle were removed and weighted. The testis were fragmented (2mm) and put in perfusion solution. For studies with light microscope, the fragments were processed routinely for inclusion in plastic resin with glycol methacrylate. Histologic cuts (4 μm) were stained in blue of toluidine/borate of sodium (1%) and analyzed. On the Experiment 1, the testis weight of animals treated with 5mg/kg was higher (13%) than those observed in the animals treated with biggest dose. The seminal vesicle of the animals treated with 5mg/kg presented increase of 17% and 26% in weight in relation to the 1mg/kg and control groups, respectively. The net weight of testis had significant reduction in the group treated with 10 mg/kg when compared to the group treated with 5mg/kg. The seminiferous tubule and epithelium volumes increased in the group of 5mg/kg when compared to the control and 10mg/kg groups. The number of Sertoli cell per transversal section had significant reduction in the groups treated with 5mg/kg and 10mg/kg when compared to control and 1mg/kg groups. Spermatides rounded by transversal section had numerical reduction in the group treated with 10mg/kg when compared with animals treated with 1mg/kg. The index of Sertoli cell (ICS) significantly increased in the animals treated with 5mg/kg in comparison to the control group. On the animals that received increased doses of pentoxifylline between 90 and 150 days of life, the higer doses of pentoxifylline had decrease of testicular parenchyma on the lume of seminiferous tubules and increased the proper tunic theses tubules. On the intertubular compartment, the highest doses of this PDE’s inhibitor increased the conjutive tissue volume and decreased the lymphatic space. The volumetry of Leydig cells increased on the treated groups with the doses of 30 and 60 mg/kg de pentoxifyllina when compared with animals treated with lower doses. The number of spermatogonia A increased on the 30 and 60 mg/kg when compared to control group. In accordance with the results, the use of the pentoxifylline during the critical period of the neonatal testis development in Wistar rats was not capable to induce increase in the population of Sertoli cells, as well the pentoxifylline during 60 days in adult Wistar rats was not capable to increase the intrinsic income of spermatogenesis and sperm production. Objetivou-se estudar a influência da pentoxifilina no desenvolvimento testicular durante o período neonatal e no processo espermatogênico de ratos Wistar adultos. Foram utilizados 37 ratos neonatos Wistar, os quais foram submetidos aos diversos tratamentos, durante os primeiros 21 dias de vida, de acordo com o grupo experimental: controle (n=10), 1mg/kg (n=10), 5mg/kg (n=9) e 10mg/kg (n=8) de pentoxifilina. Para o segundo experimento, foram utilizados 39 ratos Wistar adultos, os quais foram submetidos aos diversos tratamentos, entre 90 e 150 dias de vida, de acordo com o grupo experimental: controle (n=9), 15mg/kg (n=10), 30mg/kg (n=10) e 60mg/kg (n=10) de pentoxifilina. A pesagem dos animais foi realizada diariamente, nos grupos controle e tratados de cada experimento. Para a realização das análises histológicas e histométricas, os ratos de cada experimento foram submetidos à heparinização e anestesia e, posteriormente, submetidos à perfusão intracardíaca com solução fisiológica de NaCl a 0,9%, acrescida de heparina sódica e nitroprussiato. Após a lavagem do sistema vascular, os animais foram perfundidos com solução fixadora de glutaraldeído a 4%, em tampão fosfato de sódio, pH 7,2 e 0,01M. Posteriormente, os testículos, epidídimos e vesícula seminal foram removidos e pesados. Os testículos foram seccionados em fragmentos de 2mm e refixados na mesma solução de perfusão. Para os estudos ao microscópio de luz, os fragmentos foram processados rotineiramente para inclusão em resina plástica à base de glicol metacrilato. Cortes histológicos de 4 μm de espessura foram corados em azul de toluidina/borato de sódio a 1% e analisados. Em relação ao primeiro experimento, o peso testicular dos animais tratados com 5mg/kg foi maior 13,3% do que o observado nos animais tratados com a maior dose. O número de células de Sertoli por secção transversal sofreu redução significativa nos grupos tratados com 5mg/kg e 10mg/kg em relação aos grupos controle e tratado com 1mg/kg. O índice de célula de Sertoli (ICS) aumentou significativamente nos animais tratados com 5mg/kg em comparação ao grupo controle. Em se tratando do segundo experimento, as maiores doses de pentoxifilina promoveram redução no volume do parênquima testicular alocado no lume dos túbulos seminíferos e aumentaram a túnica própria destes túbulos. A volumetria das células de Leydig aumentou nos grupos tratados com as doses de 30 e 60mg/kg de pentoxifilina quando comparada com os animais tratados com a menor dose. Conclui-se que a utilização da pentoxifilina durante o período crítico do desenvolvimento testicular neonatal em ratos Wistar não foi capaz de induzir aumento na população das células de Sertoli, assim como a utilização da pentoxifilina durante 60 dias em ratos Wistar adultos não foi capaz de aumentar o rendimento intrínseco da espermatogênese e produção espermática.

Submitted by (edna.saturno@ufrpe.br) on 2016-10-11T14:42:47Z No. of bitstreams: 1 Guadalupe de Carvalho Xavier.pdf: 1269249 bytes, checksum: 9e9b65665bfe96096243db3a76a3bafa (MD5) Made available in DSpace on 2016-10-11T14:42:47Z (GMT). No. of bitstreams: 1 Guadalupe de Carvalho Xavier.pdf: 1269249 bytes, checksum: 9e9b65665bfe96096243db3a76a3bafa (MD5) Previous issue date: 2007-02-27 Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq Aiming to evaluate the effect of diet supplementation with Selenium and Vitamin E (Selevit E®) on the testis and seminal parameters of goats induced to scrotal insulation, it were used 12 animals with age varying between seven and eight months, distributed randomly in two groups (G1= Control; G2= Selenium and Vitamin E) and submitted to adaptation period of 60 days. In this period the animals were submitted to two semen harvests and weighted each seven days to supplement and diet correction on the G2 animals. Before putting the testis plastic bags to SI, it was measured scrotal circumference of all animals. Animals of both groups were induced to SI during 18 days, period of which they were submitted to six semen harvests, one before SI (0d), one at the finish of SI (18d) and four on the post-insulation period (PSI). At the end of SI, it was chosen randomly three animals of each group to be submitted to bilateral orchiectomy. The G2 animals have diet supplementation with Selenium and Vitamin E during this period. The maintenance diet was offered to both groups during 42 days, corresponding to post-scrotal insulation (PSI). At the end of this period, the animals of G1 and G2 groups also were orchietomized to have biometric and volumetric testis parameters, where left and right testis as well as left and right epididymis were weighted and prepared to biometric and histometric analyses. The semen harvests were done by eletroejaculation method. The Seleniun and Vitamin E group animals have significantly high scrotal circumference at the end of SI, whereas the Selenium and Vitamin E group had values significantly higher of scrotal circumference at the end of SI, whereas the Control animals were more sensitive to degenerative effects of the SI on the same period. The SI time had significant effect on the quanti-qualitative characteristics of the semen on the animals of both groups (G1 and G2), with reduction of the evaluated parameters (PM, vigor, sperm concentration, acrosoma and DNA integrity), except to semen volume. At the same way it was observed damages like testis degeneration on orchiectomized animals of both groups. However, there was significant difference on the biometric and volumetric parameters of testis parenchyma only to blood vessel volume at this period. In contrast, the serum testosterone concentration increased highly at the end of SI and differed between groups (P

Submitted by (edna.saturno@ufrpe.br) on 2016-10-13T16:09:33Z No. of bitstreams: 1 Karen Mascaro Goncalves da Silva.pdf: 349376 bytes, checksum: df0b08aa2b38c52538bbf3e08f2e8413 (MD5) Made available in DSpace on 2016-10-13T16:09:33Z (GMT). No. of bitstreams: 1 Karen Mascaro Goncalves da Silva.pdf: 349376 bytes, checksum: df0b08aa2b38c52538bbf3e08f2e8413 (MD5) Previous issue date: 2007-02-26 Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES To evaluate the effect of the season in the sperm quality of the equine semen and the use of antioxidant substances in the extender before and after thawed, three experiments was performed. In the Exp. 1, in natura semen of three Mangalarga Marchador stallions, in the breeding season (BS) and not breedii station (NBS), which had been frooze in machine using comrnercial extender FR.5 (Tris-egg yolk with glicerol 5%). After thawed at 37 'C for 30 sec.. the samples had been evaluated to volume (Vo),concentration (Co), total motility (TM) and progressive motility (MP), vigor (v), acrossomal (Aci) and DNA (DNAi) integrity, in BS and NBS, as well as proteins of sperm membrane of in natura semen. The Vo and the Co at NBS were higher than BS.TM and PM in the in natura semen in the BS were higher (P

Submitted by (edna.saturno@ufrpe.br) on 2016-08-11T13:06:12Z No. of bitstreams: 1 Ana Lydia Vasco A Peixoto.pdf: 2392494 bytes, checksum: da169ddb32ab0f7830d6e65c01995e93 (MD5) Made available in DSpace on 2016-08-11T13:06:12Z (GMT). No. of bitstreams: 1 Ana Lydia Vasco A Peixoto.pdf: 2392494 bytes, checksum: da169ddb32ab0f7830d6e65c01995e93 (MD5) Previous issue date: 2007-02-12 The antioxidant substances prevent the oxidative damages during freezing/thawing processes and incubation period caused by ROS to sperm cells, improving the sperm parameters (vigor, motility and acrosome integrity), besides avoid damages to DNA. The aim of this study was to evaluate the effect of vitamin C and Trolox addition to diluent used to ram semen cryopreservation. The ejaculates of eleven rams were harvested by artificial vagina. After macrocospic (aspect, color and viscosity) and microscopica (motility and vigor) evaluation the pool was diluted in Tris-egg yolk (Exp. 1, 2 and 3) and Fiser (Exp. 2) and supplemented with antioxidant substances: G1) Diluent (Control); G2) Diluent + 600 mM/L of vitamin C; G3) Diluent + 60 mM/L of Trolox and G4) Diluent + 600 mM/L of vitamin C + 60 mM/L of Trolox. The freezing was done by automatized method using two curves: fast(C2= –0.5 oC/minute of 25 to 5 oC, and -12.5 oC/minute of 5 to –120 oC) to the Exps. 1 and 2, and slow (C1= –0.25 oC/minute of 25 to 5 oC, and –20 oC/minute of 5 to –120 oC) to the Exp. 2, and by conventional method (90 minutes), where after the refrigeration (5 °C) the samples were placed in liquid nitrogen during 10 minutes until reaching -120 oC (Exp. 2 and 3). The straws with 100 (Exp. 1), 75 (Exp. 2 and 3) and 200 (Exp. 2) x 106 spermatozoa were transferred into the liquid nitrogen storage container (-196 oC). The semen samples were evaluated after thawing (0 min; Exp. 1, 2, and 3) and after 30 (Exp. 1 and 2) and 60 min (Exp. 1, 2 and 3) of the incubation at 37 ºC according to progressive motility, vigor, oxidative stress, acrosome and DNA integrity (Exp. 1, 2 and 3) and sperm kinetics (MT, MT, VSL,VCL, VAP, LIN, STR, ALH, and BCF; Exp. 3). In the Exp. 1, there was significant difference (p0.05). However, in the Exp. 2 the percentages of PM, vigor and acrosome integrity had significant difference (p0,05). Todavia, no Exp. 2 os porcentuais para MP, vigor e integridade de acrossoma diferiram (p

Submitted by (edna.saturno@ufrpe.br) on 2016-11-03T15:47:06Z No. of bitstreams: 1 Suely Alves de Lima.pdf: 693035 bytes, checksum: 5d20ea90b7c3198f2a791b8b01b1996f (MD5) Made available in DSpace on 2016-11-03T15:47:06Z (GMT). No. of bitstreams: 1 Suely Alves de Lima.pdf: 693035 bytes, checksum: 5d20ea90b7c3198f2a791b8b01b1996f (MD5) Previous issue date: 2006-03-28 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES Two experiments were carried out at the farm of University of Western Australia, in Perth, Australia, with the objective of studying the temperament of Merino ewes in response to male effect. In the first one, 30 females were utilized (15 calm and 15 nervous), having as probable parameters of evaluation the changes in the secretion profile of LH. Another group of 30 ewes was also equally distributed according to the temperament (15 calms and 15 nervous) and was studied to evaluate the response to male effect through the analysis of the profile of progesterone production. The results showed that calm ewes exhibited a higher response to male effect (P0,05) related to weekly estrus distribution identified by the rams (P>0,05) neither in relation to the percentage of pregnancy detected by real time ultrasound (P>0,05). The experiment was done in the experimental farm of Embrapa Tabuleiros Costeiros, in Sergipe State. Santa Inês ewes were utilized from three different categories: post-partum (G1), maiden (G2) and single ewes (G3) which were exposed to male effect through the use of teasers for 45 days. Post-partum females showed a response to male effect similar to that observed in ewes during the season anoestrus, with the concentration of first estrus between Day 17 and 25 after teasers exhibition. On the other hand, maiden and single ewes presented a random estrus distribution concentrated in the first 18 days after the introduction of males. These results confirm the efficiency of male effect on anoestrus females, as well as indicate that animals with a calm temperament presented a higher response to this stimulation. Dois experimentos foram realizados na fazenda da Universidade de Western Australia, em Perth, Austrália, com o objetivo de estudar o temperamento de ovelhas Merino em resposta ao efeito macho. No primeiro, foram utilizadas 30 fêmeas (15 calmas e 15 nervosas), tendo como parâmetro de avaliação as prováveis mudanças no perfil de secreção de LH (Hormônio Luteinizante). Um outro grupo de 30 ovelhas, eqüitativamente distribuído de acordo com o temperamento (15 calmas e 15 nervosas) foi estudado para se avaliar a resposta ao efeito macho, através da análise do perfil de produção de progesterona. Os resultados mostraram que as ovelhas calmas exibiram uma maior resposta ao efeito macho (P0,05) quanto à distribuição dos estros semanais identificados pelos carneiros com marcadores, nem com relação ao porcentual de prenhez detectada por ultra-sonografia (P>0,05). O terceiro experimento foi executado no campo experimental da Embrapa Tabuleiros Costeiros, em Sergipe. Foram utilizadas ovelhas Santa Inês de três diferentes categorias, pós-parto (G1), ovelhas jovens (G2) e solteiras (G3) que foram expostas ao efeito macho com o uso de rufiões, durante 45 dias. As fêmeas no pós-parto apresentaram uma resposta ao efeito macho próxima daquela observada em ovelhas no anestro estacional, concentrando o primeiro estro entre o 17º e o 25º dia após a exposição aos rufiões. Enquanto as ovelhas jovens e ovelhas solteiras exibiram uma distribuição do primeiro estro aleatória e concentrada nos primeiros 18 dias após a introdução dos machos. Esses resultados confirmam a eficiência do efeito macho em fêmeas em anestro, assim como indicam que animais de temperamento calmo apresentaram uma maior resposta a esta bioestimulação.

Submitted by (edna.saturno@ufrpe.br) on 2016-11-09T12:30:04Z No. of bitstreams: 1 Zoraide Fernandes Coleto.pdf: 876143 bytes, checksum: b13d1564a69ff252704812c9a3bbebcd (MD5) Made available in DSpace on 2016-11-09T12:30:05Z (GMT). No. of bitstreams: 1 Zoraide Fernandes Coleto.pdf: 876143 bytes, checksum: b13d1564a69ff252704812c9a3bbebcd (MD5) Previous issue date: 2006-03-09 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES Antioxidants act as protective agents of spermatozoa preventing the reactive oxygen species formation and consequent lipidic peroxidation rising the motility and the vigor and avoiding the damage provoked to the DNA. The aiming of this work was to study the effect of different concentrations of vitamin C and Trolox (water soluble analogue of vitamin E) on the viability of dog sperm cells submitted to the cryopreservation process. The Pool with the second semen fraction of four dogs, harvested through digital manipulation was supplemented with vitamin C and Trolox on the concentration of 200 X 106 sperm cells/dose, according to each group: Exp 1: G1= Tris- egg yolk (Control); G2 = Tris-egg yolk + 1200M of vitamin C; G3= Tris-egg yolk + 2400M of vitamin C; G4= Tris-egg yolk + 100M of Trolox e G5= Tris-egg yolk + 200M of Trolox; Exp 2: G1 = Tris-egg yolk (Control); G2 = Tris-egg yolk + vitamin C (1200M); G3 = Tris-egg yolk + Trolox (200M) and G4 = Tris-egg yolk + vitamin C (1200M) + Trolox (200M); Exp 3: G1 = Tris-egg yolk or Kenney (Control); G2 = Tris-egg yolk or Kenney + vitamin C (1200 M); G3= Tris-egg yolk or Kenney + Trolox (200 M); G4= Tris-egg yolk or Kenney + vitamin C (1200 M) + Trolox (200 M). The samples were frozen using two freezing curves (Curve 1 = -0,25oC/min until 5 oC, -15 oC/min until -5 oC, -10 oC/min until –120 oC; Curve 2 = -0,25 oC/min until 5 oC, -20 oC/min until -5 oC, -20 oC/min until –120 oC), being C1 on the 1, 2, and 3 Experiments and C2 on the 3 Experiment. The samples were stored in liquid nitrogen. After thawed, it was obshigher (P