Uso de sacarose como suplemento em diluidor para criopreservação de sêmen de garanhões

Submitted by Mario BC (mario@bc.ufrpe.br) on 2018-06-14T13:12:29Z No. of bitstreams: 1 Thalles Cloves Maciel de Moura.pdf: 598623 bytes, checksum: 1334de126ab769ec1f7cbbe5e338795e (MD5) Made available in DSpace on 2018-06-14T13:12:29Z (GMT). No. of bitstreams: 1 Thalles Cloves Maciel de Moura.pdf: 598623 bytes, checksum: 1334de126ab769ec1f7cbbe5e338795e (MD5) Previous issue date: 2017-08-02 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES The objective of the study was to investigate the effects of different concentrations of sucrose on equine semen quality after thawing. Twenty-four ejaculates were obtained from 6 Mangalarga Marchador and Campolina stallions, in which each ejaculate was divided into 5 groups: control (freezing diluent without sucrose), positive control (Botucrio®®) and three groups consisting of the freezing diluent supplemented with Saccharose at concentrations of 25 mM, 50 mM and 100 mM. The semen samples were obtained with artificial vagina, evaluated and transported (5 ºC) to the laboratory. The semen samples were diluted (200x106 sperm / mL), packed in vales and subjected to freezing. Immediately after thawing (37 ° C, 30s), the semen samples were evaluated for kinetics, plasma and acrosomal membrane integrity, membrane stability and mitochondrial membrane potential. The addition of 50 and 100 mM sucrose to the freezing diluent increased (P