224 results on '"Fulin Chen"'
Search Results
52. Glycogen synthase kinase-3β: a promising candidate in the fight against fibrosis
- Author
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Zhenlong Xin, Zhi Yang, Hongbo Liu, Fulin Chen, Yang Yang, Jihong Cui, Hanxue Zheng, and Yuan Yu
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0301 basic medicine ,Aging ,Glycogen synthase kinase-3β ,Epithelial-Mesenchymal Transition ,Medicine (miscellaneous) ,Review ,Kidney ,Extracellular matrix ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Mediator ,Fibrosis ,GSK-3 ,medicine ,Animals ,Humans ,Epithelial–mesenchymal transition ,Glycogen synthase ,Lung ,Protein Kinase Inhibitors ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Glycogen Synthase Kinase 3 beta ,biology ,Signaling pathway ,Kinase ,Myocardium ,medicine.disease ,Cell biology ,Disease Models, Animal ,030104 developmental biology ,Liver ,030220 oncology & carcinogenesis ,biology.protein ,Signal transduction ,Signal Transduction - Abstract
Fibrosis exists in almost all organs/tissues of the human body, plays an important role in the occurrence and development of diseases and is also a hallmark of the aging process. However, there is no effective prevention or therapeutic method for fibrogenesis. As a serine/threonine (Ser/Thr)-protein kinase, glycogen synthase kinase-3β (GSK-3β) is a vital signaling mediator that participates in a variety of biological events and can inhibit extracellular matrix (ECM) accumulation and the epithelial-mesenchymal transition (EMT) process, thereby exerting its protective role against the fibrosis of various organs/tissues, including the heart, lung, liver, and kidney. Moreover, we further present the upstream regulators and downstream effectors of the GSK-3β pathway during fibrosis and comprehensively summarize the roles of GSK-3β in the regulation of fibrosis and provide several potential targets for research. Collectively, the information reviewed here highlights recent advances vital for experimental research and clinical development, illuminating the possibility of GSK-3β as a novel therapeutic target for the management of tissue fibrosis in the future.
- Published
- 2020
53. CD226 deletion improves post-infarction healing via modulating macrophage polarization in mice
- Author
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Jun Li, Yun Song, Jingyi Jin, Congye Li, Guoxu Zheng, Yong-Zheng Guo, Ya-Jie Lu, Lihua Chen, Jin-Rui Zhang, Min Jia, Jianming Pei, Shumiao Zhang, Jinglong Zhang, Feng Fu, Hong-Yu Yi, Chao Gao, Guo-Hua Li, Lu Yang, and Fulin Chen
- Subjects
Antigens, Differentiation, T-Lymphocyte ,0301 basic medicine ,CD31 ,Angiogenesis ,CD226 ,Myocardial Infarction ,Macrophage polarization ,Medicine (miscellaneous) ,Spleen ,Inflammation ,macrophage ,030204 cardiovascular system & hematology ,Monocytes ,Mice ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Animals ,Ventricular remodeling ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Mice, Knockout ,polarization ,Wound Healing ,Ventricular Remodeling ,business.industry ,Macrophages ,Myocardium ,Endothelial Cells ,Macrophage Activation ,healing ,medicine.disease ,Mice, Inbred C57BL ,Phenotype ,030104 developmental biology ,medicine.anatomical_structure ,inflammation ,Cancer research ,medicine.symptom ,business ,Wound healing ,Research Paper - Abstract
Macrophages are essential for wound repair after myocardial infarction (MI). CD226, a member of immunoglobulin superfamily, is expressed on inflammatory monocytes, however, the role of CD226 in infarct healing and the effect of CD226 on macrophage remain unknown. Methods: Wild type and CD226 knockout (CD226 KO) mice were subjected to permanent coronary ligation. CD226 expression, cardiac function and ventricular remodeling were evaluated. Profile of macrophages, myofibroblasts, angiogenesis and monocytes mobilization were determined. Results: CD226 expression increased in the infarcted heart, with a peak on day 7 after MI. CD226 KO attenuated infarct expansion and improved infarct healing after MI. CD226 deletion resulted in increased F4/80+ CD206+ M2 macrophages and diminished Mac-3+ iNOS+ M1 macrophages accumulation in the infarcted heart, as well as enrichment of α-smooth muscle actin positive myofibroblasts and Ki67+ CD31+ endothelial cells, leading to increased reparative collagen deposition and angiogenesis. Furthermore, CD226 deletion restrained inflammatory monocytes mobilization, as revealed by enhanced retention of Ly6Chi monocytes in the spleen associated with a decrease of Ly6Chi monocytes in the peripheral blood, whereas local proliferation of macrophage in the ischemic heart was not affected by CD226 deficiency. In vitro studies using bone marrow-derived macrophages showed that CD226 deletion potentiated M2 polarization and suppressed M1 polarization. Conclusion: CD226 expression is dramatically increased in the infarcted heart, and CD226 deletion improves post-infarction healing and cardiac function by favoring macrophage polarization towards reparative phenotype. Thus, inhibition of CD226 may represent a novel therapeutic approach to improve wound healing and cardiac function after MI.
- Published
- 2020
54. Copper‐Catalyzed Cyclization of Aryl Amines and Aryldiazonium Salts under Air: Access to N ‐2‐Aryl‐Naphthotriazoles
- Author
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Chi Liu, Huanfeng Jiang, Fulin Chen, Chuanle Zhu, and Hao Zeng
- Subjects
chemistry.chemical_compound ,Chemistry ,Aryl ,Polymer chemistry ,Copper catalyzed ,chemistry.chemical_element ,General Chemistry ,Copper - Published
- 2019
55. Enhanced CO2 hydrate formation via biopromoter coupled with initial stirring activation
- Author
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Yongtao Zhang, Fulin Chen, Yan He, and Fei Wang
- Subjects
Fuel Technology ,General Chemical Engineering ,Organic Chemistry ,Energy Engineering and Power Technology - Published
- 2022
56. Assessment of Financial Toxicity Among Patients With Advanced Lung Cancer in Western China
- Author
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Tianqi Xu, Leidi Xu, Hangtian Xi, Yong Zhang, Ying Zhou, Ning Chang, Wenhui Yang, Yan Zhang, Ming Wang, Qing Ju, Xuemin Yang, Xiangxiang Chen, Yinggang Che, Fulin Chen, Shuoyao Qu, and Jian Zhang
- Subjects
China ,Lung Neoplasms ,financial toxicity ,Adolescent ,medical cost ,Public Health, Environmental and Occupational Health ,Financial Stress ,financial burden ,lung cancer ,Cost of Illness ,Quality of Life ,Humans ,health-related quality of life (HRQL) ,Public Health ,Public aspects of medicine ,RA1-1270 ,Original Research - Abstract
Background: Lung cancer is the leading source of cancer-caused disability-adjusted life years. Medical cost burden impacts the well-being of patients through reducing income, cutting daily expenses, curtailing leisure activities, and depleting exhausting savings. The COmprehensive Score for Financial Toxicity (COST) was created and validated by De Souza and colleagues. Our study intends to measure the financial burdens of cancer therapy and investigate the link between financial toxicity and health-related quality of life (HRQoL) in an advanced lung cancer population.Methods: Patients aged ≥ 18 years with confirmed stage III to IV lung cancer were eligible. The COST questionnaire verified by de Souza et al. was used to identify financial toxicity. Multivariable linear regression analysis with log transformation univariate analysis and Pearson correlations were used to perform the analysis.Results: The majority of the patients (90.8%, n = 138/152) had an annual income of $50,000 ($7,775). The cohort's insurance situation was as follows: 64.5% of the cohort had social insurance, 20.4% had commercial insurance, and 22.0% had both. Patients who were younger age (50–59, P < 0.001), employed but on sick leave, and had lower income reported increased levels of financial toxicity (P < 0.05). The risk factors for high financial toxicity: (i) younger age (50–59), (ii) Conclusion: Poorer psychological status and specific demographics are linked to increased financial toxicity (lower COST). Financial toxicity has a modest relationship with HRQoL and may have a clear link with HRQoL measurements.
- Published
- 2021
57. Characterization and Transcriptome Analysis of a Long-Chain
- Author
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Weina, Kong, Cheng, Zhao, Xingwang, Gao, Liping, Wang, Qianqian, Tian, Yu, Liu, Shuwen, Xue, Zhuang, Han, Fulin, Chen, and Shiwei, Wang
- Subjects
Biodegradation, Environmental ,transcriptome analysis ,Acinetobacter ,Acinetobacter pittii ,whole-genome sequencing ,Gene Expression Profiling ,RNA, Ribosomal, 16S ,Alkanes ,alkane hydroxylase ,long-chain n-alkanes ,lipids (amino acids, peptides, and proteins) ,Article - Abstract
Strain sw-1, isolated from 7619-m seawater of the Mariana Trench, was identified as Acinetobacter pittii by 16S rRNA gene and whole-genome sequencing. A. pittii sw-1 was able to efficiently utilize long-chain n-alkanes (C18–C36), but not short- and medium-chain n-alkanes (C8–C16). The degradation rate of C20 was 91.25%, followed by C18, C22, C24, C32, and C36 with the degradation rates of 89.30%, 84.03%, 80.29%, 30.29%, and 13.37%, respectively. To investigate the degradation mechanisms of n-alkanes for this strain, the genome and the transcriptome analyses were performed. Four key alkane hydroxylase genes (alkB, almA, ladA1, and ladA2) were identified in the genome. Transcriptomes of strain sw-1 grown in C20 or CH3COONa (NaAc) as the sole carbon source were compared. The transcriptional levels of alkB and almA, respectively, increased 78.28- and 3.51-fold in C20 compared with NaAc, while ladA1 and ladA2 did not show obvious change. The expression levels of other genes involved in the synthesis of unsaturated fatty acids, permeases, membrane proteins, and sulfur metabolism were also upregulated, and they might be involved in n-alkane uptake. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) confirmed that alkB expression was significantly induced by C20, C24, and C32, and almA induction extent by C24 and C32 was higher than that with C20. Furthermore, ladA2 expression was only induced by C32, and ladA1 expression was not induced by any of n-alkanes. In addition, A. pittii sw-1 could grow with 0%–3% NaCl or 8 out of 10 kinds of the tested heavy metals and degrade n-alkanes at 15 °C. Taken together, these results provide comprehensive insights into the degradation of long-chain n-alkanes by Acinetobacter isolated from the deep ocean environment.
- Published
- 2021
58. Dramatic promotion of wound healing using a recombinant human-like collagen and bFGF cross-linked hydrogel by transglutaminase
- Author
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Xiaoyan Zheng, Yan Li, Daidi Fan, Bing Xu, He Si, Yayuan Guo, Yihang Wang, Zhuoyue Chen, and Fulin Chen
- Subjects
Tissue transglutaminase ,0206 medical engineering ,Basic fibroblast growth factor ,Biomedical Engineering ,Biophysics ,Biocompatible Materials ,Bioengineering ,02 engineering and technology ,Cell Line ,law.invention ,Biomaterials ,Mice ,chemistry.chemical_compound ,law ,Animals ,Humans ,Medicine ,Skin repair ,Drug Carriers ,Wound Healing ,Transglutaminases ,integumentary system ,biology ,business.industry ,Hydrogels ,021001 nanoscience & nanotechnology ,020601 biomedical engineering ,Recombinant Proteins ,Cell biology ,Cross-Linking Reagents ,chemistry ,cardiovascular system ,Recombinant DNA ,biology.protein ,Fibroblast Growth Factor 2 ,Collagen ,0210 nano-technology ,Wound healing ,business - Abstract
The basic fibroblast growth factor (bFGF) plays an important role in the wound repair process. However, lacking of better biomaterials to carry bFGF still is a challenge in skin repair and regeneration. In this study, the human-like collagen (HLC) cross-linked with transglutaminase (TG) to fabricate a HLC/TG hydrogel to load bFGF. The physical properties of hydrogel, such as interior structure, mechanical property, were characterized
- Published
- 2019
59. Integrative Analysis of MicroRNAome, Transcriptome, and Proteome during the Limb Regeneration of Cynops orientalis
- Author
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Xin Xie, Jihong Cui, Jie Tang, Jiaojiao Su, Yuan Yu, and Fulin Chen
- Subjects
0301 basic medicine ,Luciferase reporter ,Proteome ,030102 biochemistry & molecular biology ,Regeneration (biology) ,Gene Expression Regulation, Developmental ,Urodela ,Extremities ,Differentially expressed mirnas ,General Chemistry ,Computational biology ,Biology ,biology.organism_classification ,Biochemistry ,Transcriptome ,Cynops orientalis ,MicroRNAs ,03 medical and health sciences ,030104 developmental biology ,microRNA ,Animals ,Regeneration ,KEGG - Abstract
Salamanders completely regenerate their limbs after amputation. Thus, these animals are unique models to investigate the mechanisms modulating regeneration in vertebrates. To investigate the influence of microRNAs (miRNAs) on newt limb regeneration, the miRNAs and mRNAs were simultaneously profiled using Illumina HiSeq 2500 System during limb regeneration of Cynops orientalis at 3, 7, 14, 30 and 42 days postamputation. A total of 203 miRNAs and 4230 mRNAs were identified to be differentially expressed. Together with the proteomic data obtained from our previous study, integrative analysis of multiple profiling data sets was performed to construct an interaction network of differentially expressed miRNAs, mRNAs and proteins. Results of GO and KEGG analyses showed that the differentially expressed miRNA targets were mainly directed to cytoskeletal remodeling and carbohydrate metabolism. The stage-specific regulation of miRNAs on their targets was analyzed by hierarchical clustering analysis and validated by qRT-PCR. The negative regulation of miR-223 and miR-133a on their targets was tested by performing dual luciferase reporter assay. The integration analysis will provide a powerful tool to identify the regulatory mechanisms of miRNAs and their targets. The results may have implications in understanding the complex mechanisms underlying newt limb regeneration.
- Published
- 2019
60. Ultrafine nanoparticles of W-doped SnO2for durable H2S sensors with fast response and recovery
- Author
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Wenjie Liang, Xiaoyan Zheng, Junfeng Hui, Tingbiao Yuan, Peng Chen, Pengjian Wang, Yuxin Zhao, Shi Hu, Yue Su, and Fulin Chen
- Subjects
Detection limit ,Materials science ,General Chemical Engineering ,Doping ,Nanoparticle ,Response time ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Durability ,Hydrothermal circulation ,0104 chemical sciences ,Hydrogen sulfide sensor ,Chemical engineering ,Particle size ,0210 nano-technology - Abstract
Ultrafine nanoparticles of W-doped SnO2 with an average diameter of 6 nm were fabricated via a facile hydrothermal method. The material shows a reduced particle size and enhanced response to H2S gas as compared to the pristine SnO2 nanoparticles. The detection limit can be down to 100 ppb while the response time and recovery time of the 5%-doped one are reduced to 17 s and 7 s respectively. In addition, the material shows impressive long-term stability of the response through 40 cycles of injection with 10 ppm H2S, which is attractive for designing a durable hydrogen sulfide sensor. The doping of W results in the reduction of size and modification of the electronic band structure of SnO2, which reduces the response time and recovery time and further improves the sensing durability of the materials.
- Published
- 2019
61. Transition-metal free selective C(α)–C(β) bond cleavage of trifluoromethyl ketones with amidines under air: facile access to 5-trifluoromethylated Imidazol-4-ones
- Author
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Chi Liu, Hao Zeng, Huanfeng Jiang, Chuanle Zhu, and Fulin Chen
- Subjects
chemistry.chemical_compound ,Trifluoromethyl ,Transition metal ,chemistry ,010405 organic chemistry ,Organic Chemistry ,Reaction system ,Molecular oxygen ,010402 general chemistry ,01 natural sciences ,Medicinal chemistry ,Bond cleavage ,0104 chemical sciences - Abstract
A novel and efficient strategy for the transformation of trifluoromethyl ketones is reported. The selective C(α)–C(β) bond cleavage of trifluoromethyl ketones is realized with amidines under air. This transition-metal free reaction system employs green molecular oxygen as an oxidant as well as an O-source, delivering various useful 5-trifluoromethylated imidazol-4-ones in high yields. Preliminary mechanism studies indicate that this reaction proceeds through a 1,2-aryl migration pathway rather than a radical mechanism.
- Published
- 2019
62. Forkhead box O4 transcription factor in human neoplasms: Cannot afford to lose the novel suppressor
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Yuehu Han, Tian Li, Fulin Chen, Jianjun Lv, Zhi Yang, Chenxi Lu, Yuan Yu, Yang Yang, and Shuai Jiang
- Subjects
0301 basic medicine ,Physiology ,Clinical Biochemistry ,Cell Cycle Proteins ,Biology ,medicine.disease_cause ,Metastasis ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,law ,Neoplasms ,medicine ,Transcriptional regulation ,Animals ,Humans ,Transcription factor ,Tumor Suppressor Proteins ,Cancer ,FOXO Family ,Forkhead Transcription Factors ,Cell Biology ,Prognosis ,medicine.disease ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,030220 oncology & carcinogenesis ,FOXO4 ,Cancer research ,Suppressor ,Carcinogenesis ,Signal Transduction - Abstract
Forkhead box O4 (FOXO4), a member of FOXO family, has been highlighted as an essential transcriptional regulator in many diverse carcinomas. Accumulated studies have demonstrated that FOXO4 is downregulated and associated with tumorigenesis, invasiveness, and metastasis of most human cancer. FOXO4 alteration is also closely linked to the prognosis of various types of cancer. The aim of this review is to comprehensively present the clinical and pathological significance of FOXO4 in human cancer. Additionally, the potential clinical applications of future FOXO4 research are discussed. Collectively, the information reviewed here should increase the potential of FOXO4 as a therapeutic target for cancer.
- Published
- 2018
63. Intermolecular C(sp 3 )−H Amination Promoted by Internal Oxidants: Synthesis of Trifluoroacetylated Hydrazones
- Author
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Hao Zeng, Zhiyi Yang, Fulin Chen, Yingying Cai, Chuanle Zhu, and Huanfeng Jiang
- Subjects
chemistry.chemical_classification ,Trifluoromethyl ,Chemistry ,010405 organic chemistry ,education ,Intermolecular force ,Hydrazone ,General Chemistry ,General Medicine ,Bond formation ,010402 general chemistry ,Medicinal chemistry ,01 natural sciences ,Catalysis ,0104 chemical sciences ,Metal ,chemistry.chemical_compound ,visual_art ,Reagent ,visual_art.visual_art_medium ,Amination - Abstract
An intermolecular C(sp3 )-H amination reaction is reported that is promoted by internal oxidants and occurs via a C=N bond formation step. This intermolecular C(sp3 )-H amination reaction of trifluoromethyl ketones and aryldiazonium tetrafluoroborates affords various valuable trifluoroacetylated hydrazones in high yields with excellent E/Z selectivities. The salient features of this reaction type is that it is free of metal, catalyst, directing groups, and azides, and that it can be carried out under mild conditions, is operationally simple and efficient, gram-scalable, and it tolerates various functional groups. Remarkably, an ectoparasites-controlling agent was smoothly synthesized on a gram scale using our method. Aryldiazonium tetrafluoroborates served as the aminating reagents as well as an internal oxidant.
- Published
- 2018
64. DDX21 promotes gastric cancer proliferation by regulating cell cycle
- Author
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Nan Wu, Yanan Pan, Yuying Han, Qiuqiu Hou, Xin Xie, Jiayi Cao, Yu Zhao, and Fulin Chen
- Subjects
Male ,0301 basic medicine ,Carcinogenesis ,Biophysics ,Mice, Nude ,Biology ,medicine.disease_cause ,Biochemistry ,DEAD-box RNA Helicases ,03 medical and health sciences ,Cyclin D1 ,Stomach Neoplasms ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Molecular Biology ,Cell Proliferation ,Mice, Inbred BALB C ,Cell growth ,Cell Cycle ,Cyclin-Dependent Kinase 2 ,Cancer ,Cell Biology ,Middle Aged ,Cell cycle ,medicine.disease ,RNA Helicase A ,030104 developmental biology ,Cancer cell ,Cancer research ,Female ,Ectopic expression - Abstract
DEAD (Asp-Glu-Ala-Asp) cassette helicase 21 (DDX21) is an ATP-dependent RNA helicase that is overexpressed in various malignancies. There is increasing evidence that DDX21 is involved in carcinogenesis and cancer progression by promoting cell proliferation. However, the functional role of DDX21 in gastric cancer is largely unknown. In this study, we observed that DDX21 was significantly up-regulated in gastric cancer tissues compared to paired adjacent normal tissues. The expression of DDX21 was closely related to the pathological stage of gastric cancer. In vitro and in vivo studies had shown that knockdown of DDX21 inhibited gastric cancer cell proliferation, colony formation, G1/S cell cycle transition and xenograft growth, while ectopic expression of DDX21 promoted these cell functions. Mechanically, DDX21 induced gastric cancer cell growth by up-regulating levels of Cyclin D1 and CDK2. Taken together, these results revealed a novel role for DDX21 in the proliferation of gastric cancer cells via the Cyclin D1 and CDK2 pathways. Therefore, DDX21 can be used as a therapeutic target for gastric cancer.
- Published
- 2018
65. Expression and Functional Characterization of c-Fos Gene in Chinese Fire-Bellied Newt Cynops Orientalis
- Author
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Du Wang, Zhaoxiang Mi, Shuai Lin, Yalong Feng, Yuan Yu, Gang Ye, Fulin Chen, and Jihong Cui
- Subjects
0301 basic medicine ,limb regeneration ,lcsh:QH426-470 ,c-Fos ,Biology ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Complementary DNA ,Genetics ,Coding region ,Cynops orientalis ,Peptide sequence ,Gene ,Genetics (clinical) ,biology.organism_classification ,Cell biology ,body regions ,Heptad repeat ,lcsh:Genetics ,030104 developmental biology ,expression patterns ,skin wound healing ,Wound healing ,030217 neurology & neurosurgery - Abstract
c-Fos is an immediate-early gene that modulates cellular responses to a wide variety of stimuli and also plays an important role in tissue regeneration. However, the sequence and functions of c-Fos are still poorly understood in newts. This study describes the molecular cloning and characterization of the c-Fos gene (Co-c-Fos) of the Chinese fire-bellied newt, Cynops orientalis. The full-length Co-c-Fos cDNA sequence consists of a 1290 bp coding sequence that encoded 429 amino acids. The alignment and phylogenetic analyses reveal that the amino acid sequence of Co-c-Fos shared a conserved basic leucine zipper domain, including a nuclear localization sequence and a leucine heptad repeat. The Co-c-Fos mRNA is widely expressed in various tissues and is highly and uniformly expressed along the newt limb. After limb amputation, the expression of Co-c-Fos mRNA was immediately upregulated, but rapidly declined. However, the significant upregulation of Co-c-Fos protein expression was sustained for 24 h, overlapping with the wound healing stage of C. orientalis limb regeneration. To investigate if Co-c-Fos participate in newt wound healing, a skin wound healing model is employed. The results show that the treatment of T-5224, a selective c-Fos inhibitor, could largely impair the healing process of newt&rsquo, s skin wound, as well as the injury-induced matrix metalloproteinase-3 upregulation, which is fundamental to wound epithelium formation. These data suggest that Co-c-Fos might participate in wound healing by modulating the expression of its potential target gene matrix metalloproteinase-3. Our study provides important insights into mechanisms that are responsible for the initiation of newt limb regeneration.
- Published
- 2021
66. Fabricating a novel HLC-hBMP2 fusion protein for the treatment of bone defects
- Author
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He Si, Xin Xie, Fulin Chen, Zhaoyue Wang, Zhen Zhang, Yihang Wang, Jiawei Wu, Daidi Fan, Zhuoyue Chen, and Lijun Shang
- Subjects
Biocompatibility ,Pharmaceutical Science ,Bone Morphogenetic Protein 2 ,02 engineering and technology ,Bone morphogenetic protein ,Pichia pastoris ,law.invention ,03 medical and health sciences ,In vivo ,law ,Osteogenesis ,Transforming Growth Factor beta ,Animals ,Cytotoxicity ,dewey570 ,030304 developmental biology ,0303 health sciences ,biology ,dewey540 ,Chemistry ,Mesenchymal stem cell ,Mesenchymal Stem Cells ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Fusion protein ,Cell biology ,Rats ,Bone Morphogenetic Proteins ,Saccharomycetales ,Recombinant DNA ,Collagen ,0210 nano-technology ,dewey660 - Abstract
Treating serious bone trauma with an osteo-inductive agent such as bone morphogenetic proteins (BMPs) has been considered as an optimized option when delivered via a collagen sponge (CS). Previous works have shown that the BMP concentration and release rate from approved CS carriers is difficult to control with precision. Here we presented the fabrication of a recombinant fusion protein from recombinant human-like collagen (HLC) and human BMP-2 (hBMP2). The fusion protein preserved the characteristic of HLC allowing the recombinant protein to be expressed in Yeast (such as Pichia pastoris GS115) and purified rapidly and easily with mass production after methanol induction. It also kept the stable properties of HLC and hBMP2 in the body fluid environment with good biocompatibility and no cytotoxicity. Moreover, the recombinant fusion protein fabricated a vertical through-hole structure with improved mechanical properties, and thus facilitated migration of bone marrow mesenchymal stem cells (MSCs) into the fusion materials. Furthermore, the fusion protein degraded and released hBMP-2 in vivo allowing osteoinductive activity and the enhancement of utilization rate and the precise control of the hBMP2 release. This fusion protein when applied to cranial defects in rats was osteoinductively active and improved bone repairing enhancing the repairing rate 3.5- fold and 4.2- fold when compared to the HLC alone and the control, respectively. There were no visible inflammatory reactions, infections or extrusions around the implantation sites observed. Our data strongly suggests that this novel recombinant fusion protein could be more beneficial in the treatment of bone defects than the simple superposition of the hBMP2/collagen sponge.
- Published
- 2020
67. Densification behavior and the interfacial reaction of Ti/β-Ti laminated composite by spark plasma sintering
- Author
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Peng Yang, Weidong Zhang, Fulin Chen, Zhenggang Wu, Daixiu Wei, and Xiaofeng Li
- Subjects
Mechanics of Materials ,Mechanical Engineering ,Materials Chemistry ,Metals and Alloys - Published
- 2022
68. Twisted quantum affinizations and quantization of extended affine Lie algebras
- Author
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Fulin Chen, Naihuan Jing, Fei Kong, and Shaobin Tan
- Subjects
Applied Mathematics ,General Mathematics ,Mathematics - Quantum Algebra ,FOS: Mathematics ,Quantum Algebra (math.QA) ,Mathematics::Representation Theory - Abstract
In this paper, for an arbitrary Kac-Moody Lie algebra $\mathfrak g$ and a diagram automorphism $\mu$ of $\mathfrak g$ satisfying certain natural linking conditions, we introduce and study a $\mu$-twisted quantum affinization algebra $\mathcal U_\hbar\left(\hat{\mathfrak g}_\mu\right)$ of $\mathfrak g$. When $\mathfrak g$ is of finite type, $\mathcal U_\hbar\left(\hat{\mathfrak g}_\mu\right)$ is Drinfeld's current algebra realization of the twisted quantum affine algebra. When $\mu=\mathrm{id}$ and $\mathfrak g$ in affine type, $\mathcal U_\hbar\left(\hat{\mathfrak g}_\mu\right)$ is the quantum toroidal algebra introduced by Ginzburg, Kapranov and Vasserot. As the main results of this paper, we first prove a triangular decomposition for $\mathcal U_\hbar\left(\hat{\mathfrak g}_\mu\right)$. Second, we give a simple characterization of the affine quantum Serre relations on restricted $\mathcal U_\hbar\left(\hat{\mathfrak g}_\mu\right)$-modules in terms of "normal order products". Third, we prove that the category of restricted $\mathcal U_\hbar\left(\hat{\mathfrak g}_\mu\right)$-modules is a monoidal category and hence obtain a topological Hopf algebra structure on the "restricted completion" of $\mathcal U_\hbar\left(\hat{\mathfrak g}_\mu\right)$. Last, we study the classical limit of $\mathcal U_\hbar\left(\hat{\mathfrak g}_\mu\right)$ and abridge it to the quantization theory of extended affine Lie algebras. In particular, based on a classification result of Allison-Berman-Pianzola, we obtain the $\hbar$-deformation of all nullity $2$ extended affine Lie algebras., Comment: 66 pages. Final version
- Published
- 2020
69. An array of 60,000 antibodies for proteome-scale antibody generation and target discovery
- Author
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Fei Lin, Xuemei Du, Weining Weng, Qinghua Nie, Yang Li, Shiwei Wang, Qingyou Xia, Zhiqing Li, Yiqiang Wang, Jian Yan, Mingqiao Wang, Nan Wang, Yang-Rui Li, L. Ma, Xiquan Zhang, Qin Zhang, Sheng-Ce Tao, Ziqing Chen, Yuemeng Wang, Zhaohui Wang, Kenneth D. Poss, Xuefan Xu, Fulin Chen, Mira I. Pronobis, Dongliang Huang, Rong Pan, Meng Xun, Zhiqiang Wang, Susan Zheng, Hou Bing, Xiaodong Zhao, Ying Zhang, Kun Wang, Yu-Xian Zhu, Wenfang Zeng, Huaping Dai, Li Jiang, Ying Lin, Yuan Yu, Yanfang Tang, Jing Geng, Guangcun Cheng, and Zheng Yuan
- Subjects
Proteome ,THP-1 Cells ,medicine.drug_class ,Immunology ,HL-60 Cells ,Immunofluorescence ,Monoclonal antibody ,Epitope ,Antibodies, Monoclonal, Murine-Derived ,Epitopes ,Jurkat Cells ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Antigen ,Research Methods ,Human Umbilical Vein Endothelial Cells ,medicine ,Animals ,Humans ,Research Articles ,030304 developmental biology ,0303 health sciences ,Multidisciplinary ,medicine.diagnostic_test ,biology ,Systems Biology ,fungi ,SciAdv r-articles ,Hep G2 Cells ,U937 Cells ,Cell biology ,Chromatin ,HEK293 Cells ,Membrane protein ,A549 Cells ,030220 oncology & carcinogenesis ,PC-3 Cells ,MCF-7 Cells ,biology.protein ,Antibody ,K562 Cells ,Peptides ,HeLa Cells ,Research Article - Abstract
A massively parallel array of monoclonal antibodies enables rapid antibody development and target discovery across species., Antibodies are essential for elucidating gene function. However, affordable technology for proteome-scale antibody generation does not exist. To address this, we developed Proteome Epitope Tag Antibody Library (PETAL) and its array. PETAL consists of 62,208 monoclonal antibodies (mAbs) against 15,199 peptides from diverse proteomes. PETAL harbors binders for a great multitude of proteins in nature due to antibody multispecificity, an intrinsic antibody feature. Distinctive combinations of 10,000 to 20,000 mAbs were found to target specific proteomes by array screening. Phenotype-specific mAb-protein pairs were found for maize and zebrafish samples. Immunofluorescence and flow cytometry mAbs for membrane proteins and chromatin immunoprecipitation–sequencing mAbs for transcription factors were identified from respective proteome-binding PETAL mAbs. Differential screening of cell surface proteomes of tumor and normal tissues identified internalizing tumor antigens for antibody-drug conjugates. By finding high-affinity mAbs at a fraction of current time and cost, PETAL enables proteome-scale antibody generation and target discovery.
- Published
- 2020
70. Novel role of forkhead box O 4 transcription factor in cancer: Bringing out the good or the bad
- Author
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Yang Yang, Shouyin Di, Fulin Chen, Shuai Jiang, Zhi Yang, Wei Hu, and Zhiqiang Ma
- Subjects
0301 basic medicine ,Cancer Research ,Carcinogenesis ,Cell Cycle Proteins ,Biology ,medicine.disease_cause ,Metastasis ,03 medical and health sciences ,Neoplasms ,medicine ,Humans ,Genes, Tumor Suppressor ,Transcription factor ,Cancer ,FOXO Family ,Forkhead Transcription Factors ,Hypoxia (medical) ,Cell cycle ,medicine.disease ,Oxidative Stress ,030104 developmental biology ,FOXO4 ,Cancer research ,Tumor Hypoxia ,medicine.symptom ,Transcription Factors - Abstract
Forkhead box O (FOXO) family has recently been highlighted as important transcriptional regulators associated with many diverse carcinomas. Although redundant functionality between FOXO family members with cancer is known, regulatory ability of FOXO4 for tumorigenesis and tumor metastasis is still on the way. FOXO4 significantly regulates cell cycle, resists oxidative stress, and responses to hypoxia. FOXO4 alteration is closely linked to the progression of human cancer. In this review, we introduce the regulation of FOXO4 in physiological and pathological conditions. Particularly, the pathophysiological processes and molecular pathways regulated by FOXO4 in the development and progression of cancer are also summarized. Moreover, whether FOXO4 acts as a tumor-suppressor or pro-tumoral factor in tumors and the potential directions of future FOXO4 research are discussed. The information reviewed here may assist in the experimental design and increase the potential of FOXO4 as a therapeutic target for cancer.
- Published
- 2018
71. Molecular cloning, characterization, and expression analysis of the three cysteine and glycine-rich protein genes in the Chinese fire-bellied newt Cynops orientalis
- Author
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Yuan Yu, Yalong Feng, Wenjun Wang, Hanxue Zheng, Juantao Feng, Fulin Chen, and Jihong Cui
- Subjects
0301 basic medicine ,Glycine ,Molecular cloning ,03 medical and health sciences ,Genetics ,Animals ,Amino Acid Sequence ,Cysteine ,Cloning, Molecular ,CSRP3 ,Gene ,chemistry.chemical_classification ,Messenger RNA ,Base Sequence ,biology ,Nuclear Proteins ,Sequence Analysis, DNA ,General Medicine ,Salamandridae ,biology.organism_classification ,Molecular biology ,Amino acid ,Cynops orientalis ,Open reading frame ,030104 developmental biology ,chemistry ,Sequence Alignment - Abstract
The cysteine- and glycine-rich protein (CRP) family members, including the cysteine- and glycine-rich protein 1 (CSRP1), cysteine- and glycine-rich protein 2 (CSRP2), and the cysteine- and glycine-rich protein 3 (CSRP3), have exhibited various cellular functions during cell development and differentiation. However, the sequences of the three CSRP genes and their functions are still poorly understood in newts. In this study, we cloned the complete open reading frame (ORF) sequences of the three CSRP genes from the Chinese fire-bellied newt, Cynops orientalis (C. orientalis). The complete ORF sequences of Co-CSRP1, Co-CSRP2, and Co-CSRP3 were 582, 582, and 576bp, respectively, and encoded 193, 193, and 191 amino acids, respectively. The deduced amino acid sequences of the three CRP members showed high similarities with that of other species, particularly, with amphibians. Co-CSRP1 was highly expressed in the kidney, limb, and stomach, however, the expression was low in the spleen, heart, intestine, liver, and tail (P
- Published
- 2018
72. Copper-catalyzed coupling of oxime acetates and aryldiazonium salts: an azide-free strategy toward N-2-aryl-1,2,3-triazoles
- Author
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Chuanle Zhu, Chi Liu, Rui Zhu, Wanqing Wu, Huanfeng Jiang, Hao Zeng, and Fulin Chen
- Subjects
Tetrafluoroborate ,010405 organic chemistry ,Carbazole ,Aryl ,Organic Chemistry ,Tetraphenylethylene ,010402 general chemistry ,Oxime ,01 natural sciences ,0104 chemical sciences ,chemistry.chemical_compound ,chemistry ,Polymer chemistry ,Copper catalyzed ,Organic chemistry ,Azide ,Bond cleavage - Abstract
An azide-free strategy for the synthesis of N-2-aryl-1,2,3-triazoles via copper-catalyzed coupling of oxime acetates and aryldiazonium tetrafluoroborates is reported. With the magic p-methoxyphenyldiazonium tetrafluoroborate as the reaction partner, this copper-catalyzed system favored the desired single electron transfer process via N–O bond cleavage of oxime acetates, while the classic single electron transfer process via C–N2 bond cleavage of aryldiazonium tetrafluoroborates was sufficiently inhibited. Significantly, various oxime acetates were suitable for this reaction, delivering the corresponding 4-substituted-N-2-aryl-1,2,3-triazoles and 4,5-disubstituted-N-2-aryl-1,2,3-triazoles in high yield. Primary mechanism studies indicated that this reaction involved a radical procedure. Furthermore, various novel and attractive tetraphenylethylene and carbazole based N-2-aryl-1,2,3-triazoles were synthesized smoothly.
- Published
- 2018
73. Targeting Gas6/TAM in cancer cells and tumor microenvironment
- Author
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Tian Li, Chao Deng, Wei Hu, Zhiqiang Ma, Fulin Chen, Shuai Jiang, Guiling Wu, Yicheng Cheng, and Yang Yang
- Subjects
0301 basic medicine ,Cancer Research ,Cancer cells ,Cell Survival ,medicine.medical_treatment ,Review ,C-Mer Tyrosine Kinase ,Biology ,lcsh:RC254-282 ,Metastasis ,Targeted therapy ,03 medical and health sciences ,0302 clinical medicine ,Cell Line, Tumor ,Neoplasms ,Proto-Oncogene Proteins ,medicine ,Biomarkers, Tumor ,Tumor Microenvironment ,Animals ,Humans ,Molecular Targeted Therapy ,Neoplasm Metastasis ,Cell Proliferation ,Neoplasm Staging ,Tumor microenvironment ,Tumour microenvironment ,c-Mer Tyrosine Kinase ,GAS6 ,Research ,Growth arrest-specific 6 ,Cancer ,Receptor Protein-Tyrosine Kinases ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Axl Receptor Tyrosine Kinase ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,Cancer cell ,Cancer research ,Molecular Medicine ,Intercellular Signaling Peptides and Proteins ,TYRO3 - Abstract
Growth arrest-specific 6, also known as Gas6, is a human gene encoding the Gas6 protein, which was originally found to be upregulated in growth-arrested fibroblasts. Gas6 is a member of the vitamin K-dependent family of proteins expressed in many human tissues and regulates several biological processes in cells, including proliferation, survival and migration, by binding to its receptors Tyro3, Axl and Mer (TAM). In recent years, the roles of Gas6/TAM signalling in cancer cells and the tumour microenvironment have been studied, and some progress has made in targeted therapy, providing new potential directions for future investigations of cancer treatment. In this review, we introduce the Gas6 and TAM receptors and describe their involvement in different cancers and discuss the roles of Gas6 in cancer cells, the tumour microenvironment and metastasis. Finally, we introduce recent studies on Gas6/TAM targeting in cancer therapy, which will assist in the experimental design of future analyses and increase the potential use of Gas6 as a therapeutic target for cancer.
- Published
- 2018
74. Nucleophilic trifluoromethylthiolation of bromoalkynones with AgSCF3: C(sp)–SCF3 bond formation towards ynonyl trifluoromethyl sulfides
- Author
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Huanfeng Jiang, Wanqing Wu, Rui Zhu, Fulin Chen, and Chuanle Zhu
- Subjects
chemistry.chemical_compound ,Trifluoromethyl ,Nucleophile ,010405 organic chemistry ,Chemistry ,Organic Chemistry ,Physical and Theoretical Chemistry ,Bond formation ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Medicinal chemistry ,0104 chemical sciences - Abstract
An AgSCF3 mediated nucleophilic trifluoromethylthiolation reaction for C(sp)–SCF3 bond formation is reported. This nucleophilic trifluoromethylthiolation reaction of bromoalkynones afforded various useful ynonyl trifluoromethyl sulfides in high yields. Interestingly, the over-addition of AgSCF3 is avoided in our approach.
- Published
- 2018
75. FOXO1/3: Potential suppressors of fibrosis
- Author
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Guozhan Jia, Wei Hu, Shuai Jiang, Fulin Chen, Tian Li, Zhi Yang, Zhiqiang Ma, Zhenlong Xin, and Yang Yang
- Subjects
Liver Cirrhosis ,0301 basic medicine ,Aging ,Pulmonary Fibrosis ,FOXO1 ,Disease ,Biology ,Kidney ,Biochemistry ,law.invention ,Extracellular matrix ,03 medical and health sciences ,Fibrosis ,law ,medicine ,Animals ,Humans ,Fibroblast ,Molecular Biology ,Lung ,Forkhead Box Protein O1 ,Myocardium ,Forkhead Box Protein O3 ,food and beverages ,medicine.disease ,Extracellular Matrix ,030104 developmental biology ,medicine.anatomical_structure ,Neurology ,Immunology ,Cancer research ,Suppressor ,Biotechnology - Abstract
Fibrosis is a universally age-related disease that involves nearly all organs. It is typically initiated by organic injury and eventually results in organ failure. There are still few effective therapeutic strategy targets for fibrogenesis. Forkhead box proteins O1 and O3 (FOXO1/3) have been shown to have favorable inhibitory effects on fibroblast activation and subsequent extracellular matrix production and can ameliorate fibrosis levels in numerous organs, including the heart, liver, lung, and kidney; they are therefore promising targets for anti-fibrosis therapy. Moreover, we can develop appropriate strategies to make the best use of FOXO1/3's anti-fibrosis properties. The information reviewed here should be significant for understanding the roles of FOXO1/3 in fibrosis and should contribute to the design of further studies related to FOXO1/3 and the fibrotic response and shed light on a potential treatment for fibrosis.
- Published
- 2018
76. Snapshot: Targeting Macrophages as a Candidate for Tissue Regeneration
- Author
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Tian Li, Fulin Chen, Jing Zhang, Yang Yang, and Zhi Yang
- Subjects
02 engineering and technology ,Biology ,Regenerative Medicine ,010402 general chemistry ,Candidate ,01 natural sciences ,Regenerative medicine ,Peripheral blood mononuclear cell ,Immune system ,Animals ,Humans ,Regeneration ,Wound Healing ,Targeting ,Tissue ,Exogenous antigen ,Guided Tissue Regeneration ,Macrophages ,Regeneration (biology) ,Snapshot ,General Medicine ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Cell biology ,Higher animals ,0210 nano-technology ,Wound healing - Abstract
Macrophages are a specific mononuclear cell group abundant in almost every organ of higher animals. This group is a pivotal part of the immune system and is involved in immune responses against exogenous antigen invasion. Recently, accumulating evidence has demonstrated that macrophages participate in wound repair and tissue regeneration. In this review, we will first introduce the influences of regeneration after injury in various tissues and organs among macrophage-depleted animal models. Second, the possible relationship between macrophages and reparation capacities will be discussed. Finally, we provide a general idea about the roles of macrophages in the injury- regeneration process and then discuss the current challenges and prospects of their clinical application. The information compiled here may be useful for regenerative research and may promote macrophages as a therapeutic target in regenerative medicine.
- Published
- 2018
77. Fluorescent probes guided by a new practical performance regulation strategy to monitor glutathione in living systems
- Author
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Shengyong Zhang, Tianyou Luo, Jianli Li, Jing Liu, Zhaohui Wang, Fulin Chen, Bing Yin, Ping Liu, and Mengyao She
- Subjects
Ester derivatives ,chemistry.chemical_compound ,Redox homeostasis ,010405 organic chemistry ,Chemistry ,Biophysics ,General Chemistry ,Glutathione ,010402 general chemistry ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,Living systems - Abstract
Glutathione (GSH) plays an important role in the body's biochemical defense system, and the detection of GSH in a physiological system is an important tool for understanding redox homeostasis. Protection–deprotection strategies have proven to be the most reliable, among existing detection methods. However, the understanding of how various electronic and steric effects influence a probe's ability to recognize a substrate is still lacking. In this study, we have analyzed various substituent effects on a GSH probe template via theoretical calculations and constructed the performance regulation and control strategy for this kind of probe. We then developed a series of guided probes using eighteen different acrylic ester derivatives to mask the fluorescence of fluorescein. The optical performance differences between the guided probes strongly supported the applicability of our proposed guiding strategy. Moreover, the positively guided probes are excellent for imaging GSH distribution in living cells and mice.
- Published
- 2018
78. Novel tissue-engineered skin equivalent from recombinant human collagen hydrogel and fibroblasts facilitated full-thickness skin defect repair in a mouse model
- Author
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Zhengyue Bian, Shuai Lin, Fulin Chen, Qian Xu, Juan Kang, Zhuoyue Chen, Yayuan Guo, Xue Wang, Jihong Cui, Zhaoxiang Mi, Xiaomin Wen, and Zhen Zhang
- Subjects
Materials science ,Biocompatibility ,Tissue transglutaminase ,Confocal ,Biocompatible Materials ,Bioengineering ,law.invention ,Biomaterials ,Mice ,In vivo ,law ,medicine ,Animals ,Humans ,Skin equivalent ,Fibroblast ,Skin ,Tissue Engineering ,biology ,Hydrogels ,Fibroblasts ,medicine.anatomical_structure ,Mechanics of Materials ,Recombinant DNA ,biology.protein ,Collagen ,Electron microscope ,Biomedical engineering - Abstract
Tissue-engineered skin equivalent (TESE) is an optimized alternative for the treatment of skin defects. Designing and fabricating biomaterials with desired properties to load cells is critical for the approach. In this study, we aim to develop a novel TESE with recombinant human collagen (rHC) hydrogel and fibroblasts to improve full-thickness skin defect repair. First, the bioactive effect of rHC on fibroblast proliferation, migration and phenotype was assayed. The results showed that rHC had good biocompatibility and could stimulate fibroblasts migration and secrete various growth factors. Then, rHC was cross-linked with transglutaminase (TG) to prepare rHC hydrogel. Rheometer tests indicated that 10% rHC/TG hydrogel could reach a oscillate stress of 251 Pa and remained stable. Fibroblasts were seeded into rHC/TG hydrogel to prepare TESE. Confocal microscope and scanning electronic microscope observation showed that seeded fibroblasts survived well in the hydrogel. Finally, the therapeutic effect of the newly prepared TESE was tested in a mouse full-thickness skin defect model. The results demonstrated that TESE could significantly improve skin defect repair in vivo. Conclusively, TESE prepared from rHC and fibroblasts in this study exhibits great potential for clinical application in the future.
- Published
- 2021
79. Genetic variations in TERC and TERT genes are associated with lung cancer risk in a Chinese Han population
- Author
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Gang Ye, Tianbo Jin, Mengdan Yan, Jingjie Li, Li Jing, Fulin Chen, Nan Tan, and Chenyang Meng
- Subjects
0301 basic medicine ,Oncology ,Linkage disequilibrium ,medicine.medical_specialty ,business.industry ,Haplotype ,medicine.disease ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,030220 oncology & carcinogenesis ,Internal medicine ,Genetic variation ,Genotype ,Genetic model ,medicine ,SNP ,Allele ,Lung cancer ,business - Abstract
The study was aimed to explore whether the TERT and TERC polymorphisms are associated with the lung cancer risk. Five TERC and TERT polymorphisms were genotyped from 554 lung cancer patients and 603 healthy controls. We used χ2 test, genetic model, linkage disequilibrium (LD) and haplotype analyses to evaluate the association between the polymorphisms and lung cancer risk. We found that the allele "C" of rs10936599 (TERC) and the allele "T" of rs10069690 (TERT) were associated with increased risk of lung cancer (OR = 1.32, 95% CI: 1.12-1.55, P = 0.001; OR = 1.41, 95% CI: 1.14-1.76, P = 0.002, respectively). The genotype of "CC" of rs10936599, genotype "CT" of rs10069690 and genotype "GG and "AG" of rs2853677 were also associated with increased the risk of lung cancer. In addition, rs10936599 under the dominant, recessive and log-additive models; rs10069690 under the dominant, overdominant and log-additive models; rs2853677 under the dominant and log-additive models were found to be associated with increased lung cancer risk. The SNP rs2242652 was found to be associated with an increased lung cancer risk under the dominant and overdominant models without adjustment. The haplotype "TA" of TERT was also associated with an increased risk of lung cancer. Our study indicated that rs10936599 (TERC) and rs10069690, rs2242652 and rs2853677 in TERT and haplotype "TA" of TERT were revealed as risk factors of lung cancer in a Chinese Han population. However, it required to verify our result and investigate the function genetic variants and mechanism of lung cancer.
- Published
- 2017
80. Design strategy and recent progress of fluorescent probe for noble metal ions (Ag, Au, Pd, and Pt)
- Author
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Jiao Chen, Quan-Quan Li, Mengyao She, Fulin Chen, Zhaohui Wang, Shengyong Zhang, Jianli Li, and Ping Liu
- Subjects
Development period ,010405 organic chemistry ,Chemistry ,Bioactive molecules ,Nanotechnology ,engineering.material ,010402 general chemistry ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,Ion ,Inorganic Chemistry ,Social life ,Human health ,Materials Chemistry ,engineering ,Noble metal ,Physical and Theoretical Chemistry - Abstract
Noble metals including Ag, Au, Pd, and Pt were widely applied in various aspects of human social life, industrial manufacture, healthcare, and scientific research. The ion form of these noble metals not only possess positive biological activity but also reveals serious biotoxicity owing to their outstanding coordination/reaction capability upon various bioactive molecules. Detecting noble metal ions in the environmental and biological systems is of great significance for environmental governance and human health. As an efficient method, assaying noble metal ions through the fluorescent probe is experiencing a vigorous development period and has emerged a large number of excellent researches. This review provides an overview of the design strategies of noble metal ion specific fluorescent probes based on various recognition mechanisms. Furthermore, the existing problems, challenges, and promising development directions in this area were presented.
- Published
- 2021
81. Copper-Catalyzed C(sp3)−H/C(sp3)−H Cross-Dehydrogenative Coupling with Internal Oxidants: Synthesis of 2-Trifluoromethyl-Substituted Dihydropyrrol-2-ols
- Author
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Hao Zeng, Wanqing Wu, Huanfeng Jiang, Fulin Chen, Chuanle Zhu, Chi Liu, and Rui Zhu
- Subjects
Trifluoromethyl ,010405 organic chemistry ,chemistry.chemical_element ,General Medicine ,General Chemistry ,010402 general chemistry ,Oxime ,01 natural sciences ,Medicinal chemistry ,Copper ,Catalysis ,0104 chemical sciences ,Pyridazine ,chemistry.chemical_compound ,chemistry ,Catalytic cycle ,Furan ,Thiophene ,Organic chemistry ,Pyrrole - Abstract
The first oxidative C(sp3)−H/C(sp3)−H cross-dehydrogenative coupling (CDC) reaction promoted by an internal oxidant is reported. This copper-catalyzed CDC reaction of oxime acetates and trifluoromethyl ketones provides a simple and efficient approach towards 2-trifluoromethyldihydropyrrol-2-ol derivatives in a highly diastereoselective manner by cascade C(sp3)−C(sp3) bond formation and cyclization. These products were further transformed into various significant and useful trifluoromethylated heterocyclic compounds, such as trifluoromethylated furan, thiophene, pyrrole, dihydropyridazine, and pyridazine derivatives. A trifluoromethylated analogue of an Aβ42 lowering agent was also synthesized smoothly. Preliminary mechanistic studies indicated that this reaction involves a copper(I)/copper(III) catalytic cycle with the oxime acetate acting as an internal oxidant.
- Published
- 2017
82. Melatonin: does it have utility in the treatment of haematological neoplasms?
- Author
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Wei Hu, Shuai Jiang, Zhi Yang, Yang Yang, Wencheng Di, Fulin Chen, Tian Li, Russel J. Reiter, and Zhiqiang Ma
- Subjects
0301 basic medicine ,Pharmacology ,endocrine system ,Leukemia lymphoma ,Hematologic Neoplasms ,Biology ,Bioinformatics ,Melatonin metabolism ,Melatonin ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Immunology ,medicine ,Circadian rhythm ,Haematological malignancy ,hormones, hormone substitutes, and hormone antagonists ,030217 neurology & neurosurgery ,medicine.drug - Abstract
Melatonin, discovered in 1958 in the bovine pineal tissue, is an indoleamine that modulates circadian rhythms and has a wide variety of other functions. Haematological neoplasms are the leading cause of death in children and adolescents throughout the world. Research has demonstrated that melatonin is a low-toxicity protective molecule against experimental haematological neoplasms, but the mechanisms remain poorly defined. Here, we provide an introduction to haematological neoplasms and melatonin, especially as they relate to the actions of melatonin on haematological carcinogenesis. Secondly, we summarize what is known about the mechanisms of action of melatonin in the haematological system, including its pro-apoptotic, pro-oxidative, anti-proliferative and immunomodulatory actions. Thirdly, we discuss the advantages of melatonin in combination with other drugs against haematological malignancy, as well as its other benefits on the haematological system. Finally, we summarize the findings that are contrary to the suppressive effects of melatonin on cancers of haematological origin. We hope that this information will be helpful in the design of studies related to the therapeutic efficacy of melatonin in haematological neoplasms. LINKED ARTICLES: This article is part of a themed section on Recent Developments in Research of Melatonin and its Potential Therapeutic Applications. To view the other articles in this section visit http://onlinelibrary.wiley.com/doi/10.1111/bph.v175.16/issuetoc.
- Published
- 2017
83. Carbonyl Ylides Derived from Palladium Carbenes: The Impressive Fluorine Effect
- Author
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Wanqing Wu, Pengquan Chen, Fulin Chen, Huanfeng Jiang, Rui Zhu, and Chuanle Zhu
- Subjects
Trifluoromethyl ,010405 organic chemistry ,Substituent ,chemistry.chemical_element ,General Chemistry ,010402 general chemistry ,01 natural sciences ,Carbonyl group ,Medicinal chemistry ,0104 chemical sciences ,chemistry.chemical_compound ,chemistry ,Fluorine ,Organic chemistry ,Carbene ,Palladium - Abstract
The first example for the generation of carbonyl ylides from palladium carbenes is reported. Systematic investigation of the substituents attached to the carbonyl group indicates that the employment of trifluoromethyl group as the substituent is very critical for the success of this transformation. The remarkable fluorine effect on thereactivity is very impressive, which allows to the construction of various trifluoromethylated oxiranes in high yields and diastereoselectivities.
- Published
- 2017
84. Profiling of glycan alterations in regrowing limb tissues ofCynops orientalis
- Author
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Fulin Chen, Liyuan Jia, Jihong Cui, Wenjun Wang, Hongmin Li, Hanxue Zheng, Yalong Feng, and Jing Zhang
- Subjects
0301 basic medicine ,Glycan ,biology ,Microarray analysis techniques ,Regeneration (biology) ,Lectin ,Dermatology ,Anatomy ,Cell biology ,03 medical and health sciences ,030104 developmental biology ,Cytoplasm ,biology.protein ,Immunohistochemistry ,Surgery ,Signal transduction ,Blastema - Abstract
Glycans are known to play important roles in molecular recognition, cell-cell adhesion, molecular trafficking, receptor activation, and signal transduction during development and regeneration. Despite numerous investigations of regenerating salamander limbs, global analysis of the precise variation of glycans during the limb regeneration process has received little attention. Here, we have used lectin microarrays and lectin histochemistry to analyze the alterations and distribution of glycans during the early stages leading to blastema formation during Cynops orientalis limb regeneration in response to limb amputation. Compared with the control group, analysis at several time points (3, 7, and 14 days postamputation) using microarrays containing 37 lectins showed that limb tissues expressed significantly different complements of glycans recognized by 9 different lectins. Postamputation limb tissues showed higher expression of two glycan structures recognized by the lectins STL and LTL and lower expression of seven glycan structures recognized by PHA-E, MAL-I, SNA, UEA-I, PHA-E + L, VVA, and GNA. We also observed significant changes in glycans specifically at 7 days postamputation, including higher binding capacity by WFA, GSL-I, and NPA and lower binding capacity by PNA, HHL, ConA, LCA, GSL-II, and PWM. Next, we validated our lectin microarray data using lectin histochemistry in limb tissues. Glycans recognized by STL and GNA showed similar changes in signal intensity to those found in the lectin microarrays, with STL staining in the cytoplasm and GNA in the cytoplasm and nucleus. Our findings are the first report of significant postamputation changes in glycans in limb tissues and suggest that those glycans perform potentially important functions during the early stages of C. orientalis limb regeneration.
- Published
- 2017
85. ITRAQ-based quantitative proteomic analysis of Cynops orientalis limb regeneration
- Author
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Xin Xie, Jihong Cui, Mei Sun, Jie Tang, Fulin Chen, Yuan Yu, Hanxue Zheng, Wenguang Liu, Lu Yin, and Wenjun Wang
- Subjects
0301 basic medicine ,Proteomics ,Proteome ,lcsh:QH426-470 ,lcsh:Biotechnology ,Cathepsin B ,03 medical and health sciences ,lcsh:TP248.13-248.65 ,Keratin ,Protein Interaction Mapping ,Genetics ,Animals ,Regeneration ,chemistry.chemical_classification ,030102 biochemistry & molecular biology ,biology ,Limb regeneration ,Regeneration (biology) ,Asporin ,Extremities ,Salamandridae ,Molecular biology ,Cell biology ,Cynops orientalis ,lcsh:Genetics ,030104 developmental biology ,chemistry ,iTRAQ ,biology.protein ,Titin ,Wound healing ,Biotechnology ,Research Article - Abstract
Background Salamanders regenerate their limbs after amputation. However, the molecular mechanism of this unique regeneration remains unclear. In this study, isobaric tags for relative and absolute quantification (iTRAQ) coupled with liquid chromatography tandem mass spectrometry (LC-MS/MS) was employed to quantitatively identify differentially expressed proteins in regenerating limbs 3, 7, 14, 30 and 42 days post amputation (dpa). Results Of 2636 proteins detected in total, 253 proteins were differentially expressed during different regeneration stages. Among these proteins, Asporin, Cadherin-13, Keratin, Collagen alpha-1(XI) and Titin were down-regulated. CAPG, Coronin-1A, AnnexinA1, Cathepsin B were up-regulated compared with the control. The identified proteins were further analyzed to obtain information about their expression patterns and functions in limb regeneration. Functional analysis indicated that the differentially expressed proteins were associated with wound healing, immune response, cellular process, metabolism and binding. Conclusions This work indicated that significant proteome alternations occurred during salamander limb regeneration. The results may provide fundamental knowledge to understand the mechanism of limb regeneration. Electronic supplementary material The online version of this article (10.1186/s12864-017-4125-4) contains supplementary material, which is available to authorized users.
- Published
- 2017
86. Complete genome sequence of a versatile hydrocarbon degrader, Pseudomonas aeruginosa DN1 isolated from petroleum-contaminated soil
- Author
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Yanpeng Li, Fulin Chen, Wen Dong, Yanling Ma, Chunqiu He, and Chao Huang
- Subjects
0106 biological sciences ,0301 basic medicine ,Whole genome sequencing ,chemistry.chemical_classification ,Strain (chemistry) ,Pseudomonas aeruginosa ,Biodegradation ,Biology ,medicine.disease_cause ,01 natural sciences ,Genome ,Microbiology ,03 medical and health sciences ,030104 developmental biology ,Plasmid ,Hydrocarbon ,chemistry ,010608 biotechnology ,Genetics ,medicine ,Gene - Abstract
Pseudomonas aeruginosa DN1 was isolated from a petroleum-contaminated soil from Changqing Oilfield with its capability to degrade high molecular weight polycyclic aromatic hydrocarbons (HMW PAHs) and crude oil. Herein, the whole genome sequence analysis of P. aeruginosa strain DN1 was reported, consisting of a size of 6,641,902 bp chromosome assembled genome (67.09 mol% G + C content) and a 317,349 bp plasmid assembled genome (57.01 mol% G + C content). According to the genome information, strain DN1 encodes various genes related to degradation of aliphatic hydrocarbons and aromatic compounds. In addition, DN1 contains gene clusters for biosynthesis and regulation of biosurfactant rhamnolipids. These genes may serve as a basis of further elucidation of the genetic background of this promising strain, and provide insights into investigating the metabolic and regulatory mechanisms of hydrocarbon biodegradation.
- Published
- 2017
87. Preclinical study of mouse pluripotent parthenogenetic embryonic stem cell derivatives for the construction of tissue-engineered skin equivalent
- Author
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Fulin Chen, Mei Sun, Wenguang Liu, Jihong Cui, Ling Wang, Lu Yin, Yang Rao, Wei Liu, and Xingrong Yan
- Subjects
0301 basic medicine ,KOSR ,Cellular differentiation ,Parthenogenesis ,Medicine (miscellaneous) ,Parthenogenetic embryonic stem cells ,Embryoid body ,Biology ,Regenerative Medicine ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,lcsh:Biochemistry ,03 medical and health sciences ,Mice ,Animals ,Cell Lineage ,lcsh:QD415-436 ,Functional ability ,Embryoid Bodies ,Skin ,lcsh:R5-920 ,Tissue Engineering ,Research ,Mesenchymal stem cell ,Cell Differentiation ,Mouse Embryonic Stem Cells ,Cell Biology ,Anatomy ,Fibroblasts ,Embryonic stem cell ,Cell biology ,Mice, Inbred C57BL ,030104 developmental biology ,Tissue-engineered skin equivalents ,Differentiation ,Molecular Medicine ,Intercellular Signaling Peptides and Proteins ,Stem cell ,lcsh:Medicine (General) ,Germ Layers ,Adult stem cell - Abstract
Background Embryonic stem cell (ESC) derivatives hold great promise for the construction of tissue-engineered skin equivalents (TESE). However, harvesting of ESCs destroys viable embryos and may lead to political and ethical concerns over their application. In the current study, we directed mouse parthenogenetic embryonic stem cells (pESCs) to differentiate into fibroblasts, constructed TESE, and evaluated its function in vivo. Methods The stemness marker expression and the pluripotent differentiation ability of pESCs were tested. After embryoid body (EB) formation and adherence culture, mesenchymal stem cells (MSCs) were enriched and directed to differentiate into fibroblastic lineage. Characteristics of derived fibroblasts were assessed by quantitative real-time PCR and ELISA. Functional ability of the constructed TESE was tested by a mouse skin defects repair model. Results Mouse pESCs expressed stemness marker and could form teratoma containing three germ layers. MSCs could be enriched from outgrowths of EBs and directed to differentiate into fibroblastic lineage. These cells express a high level of growth factors including FGF, EGF, VEGF, TGF, PDGF, and IGF1, similar to those of ESC-derived fibroblasts and mouse fibroblasts. Seeded into collagen gels, the fibroblasts derived from pESCs could form TESE. Mouse skin defects could be successfully repaired 15 days after transplantation of TESE constructed by fibroblasts derived from pESCs. Conclusions pESCs could be induced to differentiate into fibroblastic lineage, which could be applied to the construction of TESE and skin defect repair. Particularly, pESC derivatives avoid the limitations of political and ethical concerns, and provide a promising source for regenerative medicine.
- Published
- 2016
88. Copper-Catalyzed Intermolecular [4 + 2] Annulation Enabled by Internal Oxidant-Promoted C(sp
- Author
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Chuanle, Zhu, Hao, Zeng, Chi, Liu, Fulin, Chen, and Huanfeng, Jiang
- Abstract
A copper-catalyzed diastereoselective [4 + 2] cyclolization of α,β-unsaturated ketoxime acetates and trifluoromethyl ketones affords various 3-trifluoromethylated 3-hydroxy-cylcohexan-1-ones smoothly. This reaction features the selective functionalization of a less acidic C(sp
- Published
- 2019
89. Functionalization of a clustered TiO
- Author
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Qianli, Ma, Nan, Jiang, Shuang, Liang, Fulin, Chen, Liang, Fang, Xian, Wang, Jinjin, Wang, and Lihua, Chen
- Subjects
Titanium ,Osteogenesis ,Surface Properties ,Becaplermin ,Animals ,Bone Marrow Cells ,Cell Differentiation ,Mesenchymal Stem Cells ,Cell Proliferation ,Rats - Abstract
A multitude of micro- and nano-surface structures have been developed to improve the clinical performance of endosseous titanium (Ti) implants. However, most of these surface structures only simulate the topographic elements on a micro- or nano-scale. In this study, a nano-micro hierarchical TiO
- Published
- 2019
90. Ultrafine nanoparticles of W-doped SnO
- Author
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Pengjian, Wang, Junfeng, Hui, Tingbiao, Yuan, Peng, Chen, Yue, Su, Wenjie, Liang, Fulin, Chen, Xiaoyan, Zheng, Yuxin, Zhao, and Shi, Hu
- Abstract
Ultrafine nanoparticles of W-doped SnO
- Published
- 2019
91. Drinfeld type presentations of loop algebras
- Author
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Shaobin Tan, Fei Kong, Naihuan Jing, and Fulin Chen
- Subjects
Quantum affine algebra ,Loop algebra ,Applied Mathematics ,General Mathematics ,010102 general mathematics ,Subalgebra ,Universal enveloping algebra ,Type (model theory) ,Automorphism ,01 natural sciences ,Combinatorics ,High Energy Physics::Theory ,Vertex operator algebra ,Mathematics::Quantum Algebra ,Lie algebra ,Mathematics - Quantum Algebra ,FOS: Mathematics ,Quantum Algebra (math.QA) ,0101 mathematics ,Mathematics::Representation Theory ,Mathematics - Abstract
Let g \mathfrak {g} be the derived subalgebra of a Kac-Moody Lie algebra of finite-type or affine-type, let μ \mu be a diagram automorphism of g \mathfrak {g} , and let L ( g , μ ) \mathcal {L}(\mathfrak {g},\mu ) be the loop algebra of g \mathfrak {g} associated to μ \mu . In this paper, by using the vertex algebra technique, we provide a general construction of current-type presentations for the universal central extension g ^ [ μ ] \widehat {\mathfrak {g}}[\mu ] of L ( g , μ ) \mathcal {L}(\mathfrak {g},\mu ) . The construction contains the classical limit of Drinfeld’s new realization for (twisted and untwisted) quantum affine algebras [Soviet Math. Dokl. 36 (1988), pp. 212–216] and the Moody-Rao-Yokonuma presentation for toroidal Lie algebras [Geom. Dedicata 35 (1990), pp. 283–307] as special examples. As an application, when g \mathfrak {g} is of simply-laced-type, we prove that the classical limit of the μ \mu -twisted quantum affinization of the quantum Kac-Moody algebra associated to g \mathfrak {g} introduced in [J. Math. Phys. 59 (2018), 081701] is the universal enveloping algebra of g ^ [ μ ] \widehat {\mathfrak {g}}[\mu ] .
- Published
- 2019
92. Novel role of sex-determining region Y-box 7 (SOX7) in tumor biology and cardiovascular developmental biology
- Author
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Wenxia Zhang, Fulin Chen, Zhenxiao Jin, Yuehu Han, Baoping Xu, Wenwen Yang, and Wei Hu
- Subjects
0301 basic medicine ,Cancer Research ,Carcinogenesis ,Biology ,medicine.disease_cause ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Neoplasms ,microRNA ,medicine ,SOXF Transcription Factors ,Animals ,Humans ,Transcription factor ,Tumor biology ,Wnt signaling pathway ,030104 developmental biology ,Cardiovascular Diseases ,030220 oncology & carcinogenesis ,Cancer research ,Signal transduction ,Developmental biology ,Developmental Biology ,Signal Transduction - Abstract
The sex-determining region Y-box 7 (Sox7) is an important member of the SOX F family, which is characterized by a high-mobility-group DNA-binding domain. Previous studies have demonstrated the role of SOX7 in cardiovascular development. SOX7 expression could be detected in normal adult tissues. Furthermore, the expression levels of SOX7 were different in different tumors. Most studies showed the downregulation of SOX7 in tumors, while some studies reported its upregulation in tumors. In this review, we first summarized the upstream regulators (including transcription factors, microRNAs (miRNAs), long noncoding RNAs (lncRNAs) and some exogenous regulators) and downstream molecules (including factors in the Wnt/β-catenin signaling pathway and some other signaling pathways) of SOX7. Then, the roles of SOX7 in multiple tumors were presented. Finally, the significance of divergent SOX7 expression during cardiovascular development was briefly discussed. The information compiled in this study characterized SOX7 during tumorigenesis and cardiovascular development, which should facilitate the design of future research and promote SOX7 as a therapeutic target.
- Published
- 2019
93. Advances in antitumor effects of NSAIDs
- Author
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Fulin Chen, Lijun Shang, and Zhen Zhang
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Colorectal cancer ,NSAIDs ,aspirin ,Disease ,Review ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Primary prevention ,medicine ,cancer ,Intensive care medicine ,Aspirin ,Nonsteroidal ,Cancer prevention ,business.industry ,Cancer ,medicine.disease ,digestive system diseases ,030104 developmental biology ,Oncology ,chemistry ,030220 oncology & carcinogenesis ,platelets ,business ,medicine.drug - Abstract
In recent years, the reports on using nonsteroidal anti-inflammatory drugs (NSAIDs) for cancer prevention and treatment have been on the rise. In 2017, the US Preventive Services Working Group issued primary prevention guidelines on the use of NSAIDs, especially aspirin, for cardiovascular disease and colorectal cancer, and formally established the role and status of aspirin in cancer prevention. However, the mechanism of NSAIDs on preventing cancer is still not clear. In this paper, the progress of the application of NSAIDs, especially aspirin, in the prevention and treatment of tumors in recent years is summarized, and new ideas and directions for the follow-up study are also discussed.
- Published
- 2018
94. Genome Sequence and Metabolic Analysis of a Fluoranthene-Degrading Strain Pseudomonas aeruginosa DN1
- Author
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Chao Huang, Chunqiu He, Yanpeng Li, Yanling Ma, and Fulin Chen
- Subjects
0301 basic medicine ,Microbiology (medical) ,030106 microbiology ,lcsh:QR1-502 ,Microbiology ,Genome ,lcsh:Microbiology ,gene knockout ,03 medical and health sciences ,chemistry.chemical_compound ,Pseudomonas aeruginosa DN1 ,ORFS ,Gene ,Whole genome sequencing ,Fluoranthene ,fluoranthene degradation ,Strain (chemistry) ,biology ,Chemistry ,Pseudomonas ,genomic sequence analysis ,biology.organism_classification ,Open reading frame ,030104 developmental biology ,Biochemistry ,intermediate metabolites - Abstract
Pseudomonas aeruginosa DN1, isolated from petroleum-contaminated soil, showed excellent degradation ability toward diverse polycyclic aromatic hydrocarbons (PAHs). Many studies have been done to improve its degradation ability. However, the molecular mechanisms of PAHs degradation in DN1 strain are unclear. In this study, the whole genome of DN1 strain was sequenced and analyzed. Its genome contains 6,641,902 bp and encodes 6,684 putative open reading frames (ORFs), which has the largest genome in almost all the comparative Pseudomonas strains. Results of gene annotation showed that this strain harbored over 100 candidate genes involved in PAHs degradation, including those encoding 25 dioxygenases, four ring-hydroxylating dioxygenases, five ring-cleaving dioxygenases, and various catabolic enzymes, transcriptional regulators, and transporters in the degradation pathways. In addition, gene knockout experiments revealed that the disruption of some key PAHs degradation genes in DN1 strain, such as catA, pcaG, pcaH, and rhdA, did not completely inhibit fluoranthene degradation, even though their degradative rate reduced to some extent. Three intermediate metabolites, including 9-hydroxyfluorene, 1-acenaphthenone, and 1, 8-naphthalic anhydride, were identified as the dominating intermediates in presence of 50 μg/mL fluoranthene as the sole carbon source according to gas chromatography mass spectrometry analysis. Taken together, the genomic and metabolic analysis indicated that the fluoranthene degradation by DN1 strain was initiated by dioxygenation at the C-1, 2-, C-2, 3-, and C-7, 8- positions. These results provide new insights into the genomic plasticity and environmental adaptation of DN1 strain.
- Published
- 2018
95. Copper-Catalyzed Unstrained C-C Single Bond Cleavage of Acyclic Oxime Acetates Using Air: An Internal Oxidant-Triggered Strategy toward Nitriles and Ketones
- Author
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Hao Zeng, Zhiyi Yang, Fulin Chen, Wanqing Wu, Chi Liu, Chuanle Zhu, and Huanfeng Jiang
- Subjects
chemistry.chemical_classification ,Ketone ,010405 organic chemistry ,Radical procedure ,Aryl ,Organic Chemistry ,010402 general chemistry ,Cleavage (embryo) ,Oxime ,01 natural sciences ,Medicinal chemistry ,0104 chemical sciences ,chemistry.chemical_compound ,chemistry ,Copper catalyzed ,Single bond ,Bond cleavage - Abstract
A copper-catalyzed aerobic oxidative C-C single bond cleavage of acyclic unstrained oxime acetates is reported, providing various aryl nitriles and ketones in good yields. Mechanistic studies indicate a radical procedure is involved in this transformation, and the oxygen atom in the ketone products is originated from O2 in the air. Oxime acetates as an internal oxidant have been proved to be an initiator, which may promote the discovery of novel protocol for C-C bond cleavage and dioxygen activation.
- Published
- 2018
96. Author Correction: CRISPR-assisted detection of RNA–protein interactions in living cells
- Author
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Wenju Sun, Jing Ye, Xi Wang, Jilin Zhang, Kui Ming Chan, Jussi Taipale, Linbu Liao, Jian Yan, Liang Zhang, Xiaoxuan Zhu, Jingyu Li, Xiaoyu Li, Fulin Chen, Ligang Fan, and Wenkai Yi
- Subjects
Rna protein ,CRISPR ,Cell Biology ,Computational biology ,Biology ,Molecular Biology ,Biochemistry ,Biotechnology - Published
- 2021
97. Defect‐Rich Adhesive Molybdenum Disulfide/rGO Vertical Heterostructures with Enhanced Nanozyme Activity for Smart Bacterial Killing Application
- Author
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Xin Yu, Rui Ji, Xuanyu Chen, Aizhu Wang, Jing Liu, Fene Gao, Longwei Wang, Gu Zhanjun, Hao Li, Bo Li, Chunying Chen, Fulin Chen, Xiao Zhang, and Hong Zheng
- Subjects
Materials science ,Surface Properties ,Bacterial killing ,Nanotechnology ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Hydrothermal circulation ,Catalysis ,Nanomaterials ,chemistry.chemical_compound ,General Materials Science ,Disulfides ,Molybdenum disulfide ,Material synthesis ,Molybdenum ,Microbial Viability ,Bacteria ,Mechanical Engineering ,Temperature ,Adhesiveness ,Heterojunction ,021001 nanoscience & nanotechnology ,Anti-Bacterial Agents ,Nanostructures ,0104 chemical sciences ,chemistry ,Mechanics of Materials ,Graphite ,Adhesive ,0210 nano-technology - Abstract
Nanomaterials with intrinsic enzyme-like activities, namely "nanozymes," are showing increasing potential as a new type of broad-spectrum antibiotics. However, their feasibility is still far from satisfactory, due to their low catalytic activity, poor bacterial capturing capacity, and complicated material design. Herein, a facile synthesis of a defect-rich adhesive molybdenum disulfide (MoS2 )/rGO vertical heterostructure (VHS) through a one-step microwave-assisted hydrothermal method is reported. This simple, convenient but effective method for rapid material synthesis enables extremely uniform and well-dispersed MoS2 /rGO VHS with abundant S and Mo vacancies and rough surface, for a performance approaching the requirements of practical application. It is demonstrated experimentally and theoretically that the as-prepared MoS2 /rGO VHS possesses defect and irradiation dual-enhanced triple enzyme-like activities (oxidase, peroxidase, and catalase) for promoting free-radical generation, owing to much more active edge sites exposure. Meanwhile, the VHS-achieved rough surface exhibits excellent capacity for bacterial capture, with elevated reactive oxygen species (ROS) destruction through local topological interactions. As a result, optimized efficacy against drug-resistant Gram-negative and Gram-positive bacteria can be explored by such defect-rich adhesive nanozymes, demonstrating a simple but powerful way to engineered nanozymes for alternative antibiotics.
- Published
- 2020
98. Directing the Differentiation of Parthenogenetic Stem Cells into Tenocytes for Tissue-Engineered Tendon Regeneration
- Author
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Qi Wei, Xingrong Yan, Fulin Chen, Wenguang Liu, Mei Sun, Jihong Cui, Lu Yin, Wei Liu, and Yang Rao
- Subjects
Mechanical stretch ,0301 basic medicine ,Parthenogenesis ,Mice, Nude ,Biology ,Tendons ,Extracellular matrix ,03 medical and health sciences ,Translational Research Articles and Reviews ,Polylactic Acid-Polyglycolic Acid Copolymer ,Tissue Engineering and Regenerative Medicine ,Animals ,Regeneration ,Induced pluripotent stem cell ,Cell Proliferation ,Tissue Engineering ,Tissue Scaffolds ,Regeneration (biology) ,Mesenchymal stem cell ,Parthenogenetic stem cells ,Cell Differentiation ,Mesenchymal Stem Cells ,Cell Biology ,General Medicine ,Chondrogenesis ,Embryonic stem cell ,Cell biology ,Tenocytes ,Mice, Inbred C57BL ,Tendon regeneration ,Phenotype ,030104 developmental biology ,Adipogenesis ,Differentiation ,Immunology ,Stem cell ,Developmental Biology - Abstract
Uniparental parthenogenesis yields pluripotent stem cells without the political and ethical concerns surrounding the use of embryonic stem cells (ESCs) for biomedical applications. In the current study, we hypothesized that parthenogenetic stem cells (pSCs) could be directed to differentiate into tenocytes and applied for tissue-engineered tendon. We showed that pSCs displayed fundamental properties similar to those of ESCs, including pluripotency, clonogenicity, and self-renewal capacity. pSCs spontaneously differentiated into parthenogenetic mesenchymal stem cells (pMSCs), which were positive for mesenchymal stem cell surface markers and possessed osteogenic, chondrogenic, and adipogenic potential. Then, mechanical stretch was applied to improve the tenogenic differentiation of pMSCs, as indicated by the expression of tenogenic-specific markers and an increasing COL1A1:3A1 ratio. The pSC-derived tenocytes could proliferate and secrete extracellular matrix on the surface of poly(lactic-co-glycolic) acid scaffolds. Finally, engineered tendon-like tissue was successfully generated after in vivo heterotopic implantation of a tenocyte-scaffold composite. In conclusion, our experiment introduced an effective and practical strategy for applying pSCs for tendon regeneration.
- Published
- 2016
99. Effects of nutrition optimization strategy on rhamnolipid production in a Pseudomonas aeruginosa strain DN1 for bioremediation of crude oil
- Author
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Wen Dong, Meng-Yan Sun, Yanling Ma, Fulin Chen, Chunqiu He, and Kuang-Yi Ma
- Subjects
0106 biological sciences ,0301 basic medicine ,Chromatography ,Strain (chemistry) ,Chemistry ,Electrospray ionization ,Rhamnolipid ,Bioengineering ,Mass spectrometry ,01 natural sciences ,Applied Microbiology and Biotechnology ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,Bioremediation ,Sodium nitrate ,010608 biotechnology ,Critical micelle concentration ,Energy source ,Agronomy and Crop Science ,Food Science ,Biotechnology - Abstract
An indigenous rhamnolipid biosurfactant-producing Pseudomonas aeruginosa strain DN1 was isolated from petroleum-contaminated soil samples which could use crude oil as the sole carbon and energy source. A high yield of 25.9 g/L rhamnolipid mixture was achieved in a shake flask by nutrition optimization strategy based on high throughput screening of strain DN1, supplemented with palm oil and 5.0 g/L sodium nitrate at a C/N ratio of 20. These rhamnolipids reduced the surface tension of water from 45.23 to 25.88 mN/m with a critical micelle concentration of 50 mg/L, and were able to emulsify several hydrocarbons and showed excellent emulsification (100%). A total number of 6 monorhamnolipid homologues (Rha-C8-C8; Rha-C10:1-C8; Rha-C12:2; Rha-C12; Rha-C14-C16; Rha-C16-C16) and 7 dirhamnolipid homologues (Rha-Rha-C10; Rha-Rha-C12; Rha-Rha-C14; Rha-Rha-C8-C10; Rha-Rha-C10-C8:1; Rha-Rha-C10-C10; Rha-Rha-C10-C12) were detected by liquid chromatography/ mass spectrometry analysis allied to electrospray ionization mass spectrometry. In addition, strain DN1 exhibited an enhanced capacity to degrade crude oil (90.52%) by its rhamnolipids. The results indicated that the use of nutrition optimization strategy will be the most popular process strategy for high rhamnolipids productivity by this strain for in situ bioremediation of crude oil.
- Published
- 2016
100. A novel approach for the cryodesiccated preservation of tissue-engineered skin substitutes with trehalose
- Author
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Fulin Chen, Jihong Cui, Man Jiang, Xingrong Yan, Wei Liu, Lu Yin, Qi Wei, Mei Sun, and Chunli Xu
- Subjects
Skin, Artificial ,0301 basic medicine ,Wound Healing ,Materials science ,Tissue Engineering ,Trehalose ,food and beverages ,Bioengineering ,Biomaterials ,03 medical and health sciences ,chemistry.chemical_compound ,Freeze-drying ,Freeze Drying ,030104 developmental biology ,Biochemistry ,chemistry ,Tissue engineering ,Mechanics of Materials ,MTT assay ,Tissue engineered skin ,Viability assay ,Wound healing ,Incubation - Abstract
Tissue-engineered skin (TES) holds great promise for wound healing in the clinic. However, optimized preservation methods remain an obstacle for its wide application. In this experimental work, we developed a novel approach to preserve TES in the desiccated state with trehalose. The uptake of trehalose by fibroblasts under various conditions, including the trehalose concentration, incubation temperature and time, was studied. The cell viability was investigated by the MTT assay and CFSE/PI staining after cryodesiccation and rehydration. TES was then prepared and incubated with trehalose, and the wound healing effect was investigated after desiccated preservation. The results showed that the optimized conditions for trehalose uptake by fibroblasts were incubation in 200 mM trehalose at 37 °C for 8 h. Cryodesiccated cells and TES maintained 37.55% and 28.31% viabilities of controls, respectively. Furthermore, cryodesiccated TES exhibited a similar wound healing effect to normal TES. This novel approach enabled the preservation and transportation of TES at ambient temperature with a prolonged shelf time, which provides great advantages for the application of TES.
- Published
- 2016
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