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Directing the Differentiation of Parthenogenetic Stem Cells into Tenocytes for Tissue-Engineered Tendon Regeneration
- Source :
- Stem Cells Translational Medicine
- Publication Year :
- 2016
- Publisher :
- Oxford University Press (OUP), 2016.
-
Abstract
- Uniparental parthenogenesis yields pluripotent stem cells without the political and ethical concerns surrounding the use of embryonic stem cells (ESCs) for biomedical applications. In the current study, we hypothesized that parthenogenetic stem cells (pSCs) could be directed to differentiate into tenocytes and applied for tissue-engineered tendon. We showed that pSCs displayed fundamental properties similar to those of ESCs, including pluripotency, clonogenicity, and self-renewal capacity. pSCs spontaneously differentiated into parthenogenetic mesenchymal stem cells (pMSCs), which were positive for mesenchymal stem cell surface markers and possessed osteogenic, chondrogenic, and adipogenic potential. Then, mechanical stretch was applied to improve the tenogenic differentiation of pMSCs, as indicated by the expression of tenogenic-specific markers and an increasing COL1A1:3A1 ratio. The pSC-derived tenocytes could proliferate and secrete extracellular matrix on the surface of poly(lactic-co-glycolic) acid scaffolds. Finally, engineered tendon-like tissue was successfully generated after in vivo heterotopic implantation of a tenocyte-scaffold composite. In conclusion, our experiment introduced an effective and practical strategy for applying pSCs for tendon regeneration.
- Subjects :
- Mechanical stretch
0301 basic medicine
Parthenogenesis
Mice, Nude
Biology
Tendons
Extracellular matrix
03 medical and health sciences
Translational Research Articles and Reviews
Polylactic Acid-Polyglycolic Acid Copolymer
Tissue Engineering and Regenerative Medicine
Animals
Regeneration
Induced pluripotent stem cell
Cell Proliferation
Tissue Engineering
Tissue Scaffolds
Regeneration (biology)
Mesenchymal stem cell
Parthenogenetic stem cells
Cell Differentiation
Mesenchymal Stem Cells
Cell Biology
General Medicine
Chondrogenesis
Embryonic stem cell
Cell biology
Tenocytes
Mice, Inbred C57BL
Tendon regeneration
Phenotype
030104 developmental biology
Adipogenesis
Differentiation
Immunology
Stem cell
Developmental Biology
Subjects
Details
- ISSN :
- 21576580 and 21576564
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Stem Cells Translational Medicine
- Accession number :
- edsair.doi.dedup.....aa9019d00155c190dca09d053ab66b8a