51. Analysis of excreta bacterial community after forced molting in aged laying hens
- Author
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Han Na Oh, Hwan Ku Kang, Woo Jun Sul, Kyu-Chan Lee, Dong Yong Kil, and Gi Ppeum Han
- Subjects
animal structures ,High-throughput 16S rRNA Sequencing ,Firmicutes ,lcsh:Animal biochemistry ,Article ,Animal science ,Lactobacillus ,Bacterial phyla ,lcsh:QP501-801 ,lcsh:SF1-1100 ,biology ,integumentary system ,fungi ,0402 animal and dairy science ,Bacteroidetes ,04 agricultural and veterinary sciences ,Nonruminant Nutrition and Feed Processing ,biology.organism_classification ,040201 dairy & animal science ,Forced molting ,Excreta Bacterial Community ,Aged Laying Hen ,Animal Science and Zoology ,lcsh:Animal culture ,Forced Molting ,Proteobacteria ,Enterococcus cecorum ,Moulting ,Food Science - Abstract
OBJECTIVE As laying hens become aged, laying performance and egg quality are generally impaired. One of the practical methods to rejuvenate production and egg quality of aged laying hens with decreasing productivity is a forced molting. However, the changes in intestinal microbiota after forced molting of aged hens are not clearly known. The aim of the present study was to analyze the changes in excreta bacterial communities after forced molting of aged laying hens. METHODS A total of one hundred 66-wk-old Hy-Line Brown laying hens were induced to molt by a 2-d water removal and an 11-d fasting until egg production completely ceased. The excreta samples of 16 hens with similar body weight were collected before and immediately after molting. Excreta bacterial communities were analyzed by high-throughput sequencing of bacterial 16S rRNA genes. RESULTS Bacteroidetes, Firmicutes, and Proteobacteria were the three major bacterial phyla in pre-molting and immediate post-molting hens, accounting for more than 98.0%. Lactobacillus genus had relatively high abundance in both group, but decreased by molting (62.3% in pre-molting and 24.9% in post-molting hens). Moreover, pathogenic bacteria such as Enterococcus cecorum and Escherichia coli were more abundant in immediate post-molting hens than in pre-molting hens. Forced molting influenced the alpha diversity, with higher Chao1 (p = 0.012), phylogenetic diversity whole tree (p = 0.014), observed operational taxonomic unit indices (p = 0.006), and Simpson indices (p
- Published
- 2019