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The associations between autonomic function and biventricular function in patients with the indeterminate form of Chagas disease remains to be elucidated.In 42 asymptomatic patients and 19 healthy volunteers, the autonomic function was assessed by time domain indices of heart rate variability (HRV), analyzed for 24 h; the right ventricular function was assessed by fraction area change, right ventricle shortening, and systolic excursion of the tricuspid valve; and the left ventricular function was assessed by ejection fraction and transmitral flow velocities. Data were expressed as mean+/-SD or medians (including the lower quartile and upper quartile). Groups were compared by Student's t or Mann-Whitney U test. Autonomic and ventricular function were correlated by Pearson's or Spearman's correlation coefficient. The level of significance was 5%.Right and left ventricular systolic function indexes were comparable between groups. Transmitral flow velocities were decreased in the Chagas disease group (p0.05). The patients presented impaired HRV as indicated by the values of SDNN-day (80 (64-99) ms vs. 98 (78-127) ms; p=0.045), SDNNI-24 h (54 (43-71) vs. 65 (54-105) ms; p=0.027), SDNNI-day (49 (42-64) vs. 67 (48-76) ms; p=0.045), pNN50-day (2.2 (0.7-5)% vs. 10 (3-11)%; p=0.033); and pNN50-24 h (3 (1-7)% vs. 12 (8-19)%; p=0.013). There were no correlations between the left ventricular diastolic indices and autonomic dysfunctional indices (p0.05).Patients with the indeterminate form of Chagas disease have both dysautonomia and left ventricular diastolic dysfunction. However, the right ventricular function is preserved. Importantly, ventricular diastolic dysfunction and dysautonomia are independent phenomena.

To evaluate tuberculin test results and relate them to the presence or absence of active tuberculosis, as well as to CD4+ and CD8+ T-lymphocyte counts.The charts of 802 patients with acquired immunodeficiency syndrome treated between August of 1985 and March of 2003 were reviewed. Of the 185 patients submitted to tuberculin tests (23.1%), 107 (57.8%) were male, and 78 (42.2%) were female. Patients were divided into two study groups: tuberculin test reactors (n = 28); and tuberculin test non-reactors (n = 157). Among the reactors, the mean age was 30.60 years, with a standard deviation of 6.62 years, compared with 34.45 years, with a standard deviation of 10.32 years, among the non-reactors.Most of the individuals tested presented only a mild response to the tuberculin test. We found that, at the time of the test, the percentage of individuals with active tuberculosis was greater in the reactor group than in the non-reactor group. During the test period, 10 reactor group patients and 11 non-reactor group patients presented some clinical form of active tuberculosis. In addition, CD4+ T-lymphocyte counts were lower than 200 cells/mm(3) in 6 reactor group patients and in 8 non-reactor group patients.Indurations greater than 5 mm were unrelated to higher absolute CD4+ T-cell counts.

In order to asses the soroprevalence of HTLV- I/II, HIV, siphylis, and toxoplasmosis infection among pregnant women seen at Basic Health Units in the municipality of Botucatu - São Paulo - Brazil, and risk factors for HTLV- I/II infection, sorologic surveys were carried out and results of sorologic surveys carried in pregnance routine evaluation were assessed. Seroprevalence was 0.1% (1 in 913) for both HTLV-I and HTLV-II. Seroprevalence was 0.1% (1 in 913) for both HTLV-I and HTLV-II. Siphylis, toxoplasmosis and HIV were identified. None of the factors or characteristics risks was found to be useful to identify those prenant women HTLV-II infected. Infection among pregnant women and southeast brazilian blood donnors were not diferent in statistical evaluation.

Seventy-nine HIV-1 infected patients were studied in three groups: Group G1 - 11 patients with no antiretroviral therapy; G2 - 40 patients undergoing antiretroviral therapy, 33 with only two nucleoside reverse transcriptase inhibitors (NRTI), and seven with two NRTI and one protease inhibitor (PI), all with viral load (VL) equal or higher than 80 copies of plasma RNA/ml; Group G3 - 28 patients, 23 on highly active antiretroviral therapy (HAART), 18 with two NRTI and one PI, and five with two NRTI and one non-nucleoside reverse transcriptase inhibitor (NNRTI), the remaining five with combination of two NRTI. All G3 patients had undetectable viral load for at least the past six months. The control group (Gc) included 20 normal blood donors without clinical complaints or signs of disease and negative for anti-HIV-1/2 antibodies. Serum cytokine levels pg/ml (TNF-alpha, INF-gamma, IL-2, IL-4, and IL-10) were determined in all patients including controls. CD4+ T and CD8+ T lymphocyte counts were made in the 79 patients by flow cytometry; VL determination was by NASBA technology. Analysis of results showed that the number of CD4+ T and CD8+ T lymphocytes were higher in G2 than G1, while VL was 0.5 log lower. G3 patients had similar lymphocyte values to G2, however they were chosen for G3 because their VL was undetectable, different by 4.0 log to G2. These results show the effect of antiretroviral treatment in G2 and G3 patients with better performance in the latter. Statistical difference was seen between the three groups and controls for serum cytokine behavior: TNF-alpha [H=48.323; pGc]; INF-gamma[H=28.992; pGc]; IL-4[H=48.323; pGc]; IL-10[H=47.256; pGc. There was no statistical difference in IL-2 values between all groups (H=6.071; p>0.10; G1=G2=G3=Gc). In absolute values however, G3 showed slightly lower TNF-alpha, IL-4, and IL-10, and higher INF-gamma and IL-2, to G1 and G2. This suggests a better performance in G3 patients, especially in IL-2 behavior. For cytokine profile, the three groups showed mature Th0 subset. In G1 72.73% were mature Th0, and 27.27% Th2; G2, 72.50% mature Th0, and 27.50% Th2; and G3, 89.29% mature Th0, and 10.71% Th2. There was no statistical difference between groups (chi22=3.014; p>0.10; G1=G2=G3). Statistical difference was seen between G2 and G3 for antiretroviral regimes used (chi21=27.932; pG2); HAART with PI predominated in G3, suggesting that it was responsible for this better performance. Linear correlation between pairs of variables was made with patient groups only, excluding controls. This was made separately for G1 and G2, 51 patients with detectable VL, and G1, G2, and G3 also including those with undetectable VL. The results showed a strong positive correlation between TNF-alpha and IL-4; TNF-alpha and IL-10; INF-gamma and IL-2; IL-4 and IL-10; IL-2 and CD4+. Weak negative correlation was seen between IL-2 and VL. Considering all correlations together, we found that IL-2 had the most correlations - eleven - strong, weak, positive, and negative; it was the only one that correlated with CD4+ (positively) and LV (negatively). The number of correlations allowed us to evaluate qualitative aspects such as IL-2 correlated positively with INF-gamma and CD4+ and negatively with LV; this somehow expresses the compatible profile with subset Th1, which could signify a tendency towards immune response recovery. Determination of cytokine serum values, especially IL-2, could be useful in follow-up of HIV-1 infected patients under HAART together with CD4+ and VL count.

To describe the acquired immunodeficiency syndrome (AIDS) epidemic in Brazil and analyze the impact of federal government measures addressing the problem since its onset.Retrospective review of AIDS epidemic data from its onset in 1980 up to the last published data in June 2001.AIDS was first reported in Brazil in 1980. By 1988, all 27 Brazilian states had diagnosed cases, and until June 2000 more than half of Brazilian municipalities had recorded at least one case of the disease. The AIDS incidence reached its peak between 1996 and 1997 (14.7 per 100,000 population), and then declined between 1998 and 2000 to 9 per 100,000 population. In the last two decades, the proportion of deaths has been also significantly reduced. These were not random events, but reflected the efficiency of the program implemented by the Brazilian Health Ministry's Coordination on Sexually Transmitted Diseases and AIDS. The program includes an epidemiological surveillance modeling system, which records cases from several regular epidemiological bulletins; national network of diagnosis and monitoring of HIV-infected individuals (ill or not); highly active antiretroviral therapy available free to all patients; mother-infant protection program; educational programs on condom use; the introduction of the female condom; development of AIDS studies in different areas to provide practical solutions; constant preoccupation about drug costs accounting for the patent breaking; and national production of many drugs currently in use.Well-planned and implemented national program against AIDS can significantly reduce the burden of this disease to the population.

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T19:05:52Z No. of bitstreams: 1 S0037-86821995000400002.pdf: 284157 bytes, checksum: ddc51c96ffb78e8673ad2f2a11047482 (MD5) Made available in DSpace on 2013-08-22T19:05:52Z (GMT). No. of bitstreams: 1 S0037-86821995000400002.pdf: 284157 bytes, checksum: ddc51c96ffb78e8673ad2f2a11047482 (MD5) Previous issue date: 1995-12-01 Made available in DSpace on 2013-09-30T18:19:32Z (GMT). No. of bitstreams: 1 S0037-86821995000400002.pdf: 284157 bytes, checksum: ddc51c96ffb78e8673ad2f2a11047482 (MD5) Previous issue date: 1995-12-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:34:41Z No. of bitstreams: 1 S0037-86821995000400002.pdf: 284157 bytes, checksum: ddc51c96ffb78e8673ad2f2a11047482 (MD5) Made available in DSpace on 2014-05-20T13:34:41Z (GMT). No. of bitstreams: 1 S0037-86821995000400002.pdf: 284157 bytes, checksum: ddc51c96ffb78e8673ad2f2a11047482 (MD5) Previous issue date: 1995-12-01 UNESP Faculdade de Medicina de Botucatu Departamento de Doenças Tropicais e Diagnóstico por Imagem UNESP Faculdade de Medicina de Botucatu Departamento de Doenças Tropicais e Diagnóstico por Imagem

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T19:04:31Z No. of bitstreams: 1 S0036-46651995000100010.pdf: 763341 bytes, checksum: a02028edf62f655e6eab6954c10ae18b (MD5) Made available in DSpace on 2013-08-22T19:04:31Z (GMT). No. of bitstreams: 1 S0036-46651995000100010.pdf: 763341 bytes, checksum: a02028edf62f655e6eab6954c10ae18b (MD5) Previous issue date: 1995-02-01 Made available in DSpace on 2013-09-30T20:07:26Z (GMT). No. of bitstreams: 2 S0036-46651995000100010.pdf: 763341 bytes, checksum: a02028edf62f655e6eab6954c10ae18b (MD5) S0036-46651995000100010.pdf.txt: 7 bytes, checksum: 212b0306580d4f0044d18f9a3edcc832 (MD5) Previous issue date: 1995-02-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:17:14Z No. of bitstreams: 2 S0036-46651995000100010.pdf: 763341 bytes, checksum: a02028edf62f655e6eab6954c10ae18b (MD5) S0036-46651995000100010.pdf.txt: 7 bytes, checksum: 212b0306580d4f0044d18f9a3edcc832 (MD5) Made available in DSpace on 2014-05-20T15:17:14Z (GMT). No. of bitstreams: 2 S0036-46651995000100010.pdf: 763341 bytes, checksum: a02028edf62f655e6eab6954c10ae18b (MD5) S0036-46651995000100010.pdf.txt: 7 bytes, checksum: 212b0306580d4f0044d18f9a3edcc832 (MD5) Previous issue date: 1995-02-01 Os autores estudaram 32 doentes picados por serpentes venenosas, sendo 16 picados por Bothrops spp. e 16 por Crotalus durissus terrificus. Trinta doentes eram do sexo masculino e dois do feminino com idades variando entre 8 e 63 anos (méda 33±15). A prova da retenção da bromosulfaleína apresentou-se aumentada na maioria dos doentes picados por serpentes Crotalus durissus terrificus. Houve correlação positiva entre a retenção da bromosulfaleína e os níveis séricos de alanina aminotransferase e entre alanina e aspartato aminotransferase apenas nos doentes do grupo Crotalus. Um dos doentes evoluiu para o óbito e apresentou no exame anatomopatológico do fígado degeneração hidrópica e lesões mitocondriais. Os autores concluem que as alterações hepáticas são causadas por pelo menos dois mecanismos a saber: lesão mitocondrial por efeito do veneno crotálico; efeito das citoquinas, especialmente a interleucina-6. Thirty-two patients bitten by venomous snakes sixteen by Bothrops spp. and sixteen by Crotalus durissus terrificus were studied. The group comprised thirty males and two females, aged eight to sixty-three years (mean 33±15). Bromsulphalein tests were increased in the majority of patients bitten by Crotalus durissus terrificus. The correlation coefficient of Spearman was positive between bromsulphalein tests and alanine aminotransferase levels, and between alanine aminotransferase and aspartate aminotransferase levels only in the Crotalus group. The only patient who died was bitten by Crotalus durissus terrificus and showed hydropic degeneration and mitochondrial injury in the liver. It was concluded that the hepatic damage might have been caused by at least two possible mechanisms: venom effect on liver mitochondria and cytokine effects on hepatocyte, specially interleukin-6. UNESP Faculdade de Medicina de Botucatu Departamento de Doenças Tropicais e Diagnóstico CEVAP UNESP Centro de Estudos de Venenos e Animais Peçonhentos UNESP Faculdade de Medicina de Botucatu Departamento de Patologia UNESP Faculdade de Medicina Veterinária e Zootecnia de Botucatu Serviço de Estatística UNESP Faculdade de Medicina de Botucatu Departamento de Doenças Tropicais e Diagnóstico CEVAP UNESP Centro de Estudos de Venenos e Animais Peçonhentos UNESP Faculdade de Medicina de Botucatu Departamento de Patologia UNESP Faculdade de Medicina Veterinária e Zootecnia de Botucatu Serviço de Estatística

Made available in DSpace on 2021-07-14T10:38:01Z (GMT). No. of bitstreams: 0 Previous issue date: 1995 Thirty-one patients bitten by venomous snakes in Botucatu area (State of São Paulo - Brazil), sixteen by Bothrops spp. and fifteen by Crotalus durissus terrificus, were studied. The group comprised twenty-nine males and two females, ranging from fourteen to sixty-three years of age (mean 33 ± 15). Leukocytosis with neutrophilia and lymphopenia, increase of mucoproteins and C-reactive protein, decrease of total serum protein and albumin, were observed on the first day after the accident. In addition, increased serum levels of the cytokines IL-6 and IL-8, but not of IL-1b and TNF-a, were observed. The alterations were generally more intense in patients bitten by Crotalus durissus terrificus than by Bothrops spp. It is concluded that these snakebite envenomations closely resemble an acute trauma, inducing a typical acute-phase response. University of Göteborg, Sweden Universidad de Costa Rica, Instituto Clodomiro Picado, Facultad de Microbiologia , Department of Clinical Immunology School of Medicine of Botucatu, Department of Tropical Diseases CEVAP-UNESP, Center for the Study of Venoms and Venomous Animals CEVAP-UNESP, Center for the Study of Venoms and Venomous Animals

The presence of Paracoccidioides brasiliensis was determined in sputum samples from 50 patients with paracoccidioidomycosis using four different techniques: (a) cell-block preparations stained with silver methenamine, (b) direct microbiologie examination, (c) smears stained with Shorr, and (d) smears stained with silver methenamine. Overall, cell-block preparations and smears stained with silver methenamine proved to be the most sensitive techniques, followed by smears stained with Shorr and direct microbiologic examination in decreasing order of sensitivity. Sputum cytology tended to be less positive in patients with interstitial pulmonary lesions as determined by chest X-ray than in patients with alveolar lesions. In addition to its high sensitivity, cell-block preparation technique allows storage of blocks and slides for further studies.

The authors evaluated the relationships among renal function, acetylator phenotype and serum sulfadiazine levels in 22 patients with paracoccidioidomycosis treated with 1 tablet of cotrimazine (a combination of 820 mg sulfadiazine and 180 mg trimethoprim) administered orally every 12 hours. Fifteen patients (68.18%) presented free sulfadiazine levels above 50 micrograms/ml, 6(27.28%) presented serum levels above 40 micrograms/ml, and 1(4.54%), levels lower than 40 micrograms/ml, this being the patient in which treatment failed. The highest free sulfadiazine levels were obtained in slow acetylator patients with reduced renal function. One patient with neuroparacoccidioidomycosis presented free sulfadiazine levels in cerebrospinal fluid corresponding to 55% of the serum levels. Finally, the authors consider cotrimazine to be an important therapeutic alternative for neuroparacoccidioidomycosis and conclude that administration every 12 hours can provide therapeutic sulfadiazine levels. They also suggest that when the sulfadiazine-trimethoprim combination is used, the therapeutic levels of sulfadiazine should be above 40 micrograms/ml.

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T19:03:58Z No. of bitstreams: 1 S0036-46651988000500007.pdf: 1487021 bytes, checksum: 0e2401a88a0a70b9f3b328b7daa7adec (MD5) Made available in DSpace on 2013-08-22T19:03:58Z (GMT). No. of bitstreams: 1 S0036-46651988000500007.pdf: 1487021 bytes, checksum: 0e2401a88a0a70b9f3b328b7daa7adec (MD5) Previous issue date: 1988-10-01 Made available in DSpace on 2013-09-30T18:18:52Z (GMT). No. of bitstreams: 2 S0036-46651988000500007.pdf: 1487021 bytes, checksum: 0e2401a88a0a70b9f3b328b7daa7adec (MD5) S0036-46651988000500007.pdf.txt: 36021 bytes, checksum: e60fefb2f692d4995e0096154ad50957 (MD5) Previous issue date: 1988-10-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:32:34Z No. of bitstreams: 2 S0036-46651988000500007.pdf: 1487021 bytes, checksum: 0e2401a88a0a70b9f3b328b7daa7adec (MD5) S0036-46651988000500007.pdf.txt: 36021 bytes, checksum: e60fefb2f692d4995e0096154ad50957 (MD5) Made available in DSpace on 2014-05-20T13:32:34Z (GMT). No. of bitstreams: 2 S0036-46651988000500007.pdf: 1487021 bytes, checksum: 0e2401a88a0a70b9f3b328b7daa7adec (MD5) S0036-46651988000500007.pdf.txt: 36021 bytes, checksum: e60fefb2f692d4995e0096154ad50957 (MD5) Previous issue date: 1988-10-01 Os autores padronizaram métodos para a avaliação da atividade da glicose-6-fosfato desidrogenase e glutationa redutase. O princípio geral do primeiro método baseou-se na formação de metahemoglobina pelo nitrito de sódio, seguido da estimulação da via das pentoses pelo azul de metileno. Foram estudados 46 indivíduos adultos, sendo 23 do sexo masculino e 23 do feminino, não deficientes em glicose-6-fosfato desidrogenase (G6PD), com idades variando entre 20 e 30 anos. Os resultados revelaram que a redução da metahemoglobina pelo azul de metileno para sangue total, foram de 154.50 e 139.90 mg/min (p

Os autores estudaram a prevalência da deficiência de glicose-6-fosfato desidrogenase (G6PD), pelo método de BREWER et alii, em 141 indivíduos da população do município de Humaitá, Estado do Amazonas. Destes, 128 eram amazônides, 67 dos quais nunca tiveram malária, enquanto que 61 tinham tido ou estavam tendo a doença; os 13 restantes que estavam com malária não eram arnazônides. Os resultados revelaram que 7 arnazônides (4,96%), eram deficientes. Destes, 5 eram do sexo feminino e 2 do masculino. Em todos os indivíduos do sexo feminino o teste foi positivo com comportamento do tipo heterozigoto. Dos indivíduos deficientes, 4 nunca tinham tido malária; dos outros 3, 2 apresentavam a reação de hemaglutinação positiva com título 1/16 e o terceiro estava tendo malária causada pelo Plasmodium falciparum pela primeira vez. Este doente apresentou forma benigna de malária evoluindo para cura clínica e parasitológica no 3.° dia de tratamento com a clindamicina. Nenhum dos 13 doentes não arnazônides apresentava deficiência de G6PD. Dessa forma, não houve diferença na prevalência da deficiência de G6PD em arnazônides que nunca tiveram malária e em arnazônides que tinham tido ou estavam tendo a doença. Portanto, os indivíduos com deficiência de G6PD estão sujeitos a infecções por Plasmodium falciparum na mesma proporção que os não deficientes. Por outro lado, o aumento da prevalência da deficiência de G6PD, na amostra estudada, poderia estar relacionado com a pressão seletiva exercida pela malária em população submetida à homozigose. The authors studied the prevalence of glucoses-phosphate dehydrogenase (G6PD) deficiency by BREWER method et al, in 141 individuals from Humaita county, Amazonas State. From this total, 128 were born in Amazonian 67 had never had malaria, but 61 had already had this disease or were with; it the others thirteen left, who had this disease were not from Amazonian. The results revealed that 7 (4,96%) Amazonian individuals were deficients. From this total, there were 5 females and 2 males individuals. In all female individuals the test was positive according to heterozygote kind of behaviour. Among the deficient individuals, four had never had malaria from the 3 left, 2 presented a positive hemagglutination reaction with 1/16 tittle and the third was with malaria for the first time, caused by Plasmodium falciparum. This patient presented the benign form of malaria followed by clinical and parasitological remission in the third day of treatment with clindamycin. None of the 13 patients who weren't Amazonian individuals presented G6PD deficiency. Being so, there were no diference in the prevalence of G6PD in Amazonian individuals who had never had the disease and in Amazonian individuals who had already had or were with the disease. Thus, the individuals with G6PD deficiency are subject to contamination by Plasmodium falciparum in the same proportion to the no deficients. In the other hand, the increase of prevalence of G6PD deficiency in the sample studied, could be related to the seletive pressure on malaria in population submited to homozygous.

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T19:04:02Z No. of bitstreams: 1 S1413-86702007000200012.pdf: 220443 bytes, checksum: 2c23469da3d575381bc17a3d982f0661 (MD5) Made available in DSpace on 2013-08-22T19:04:02Z (GMT). No. of bitstreams: 1 S1413-86702007000200012.pdf: 220443 bytes, checksum: 2c23469da3d575381bc17a3d982f0661 (MD5) Previous issue date: 2007-04-01 Made available in DSpace on 2013-09-30T18:19:31Z (GMT). No. of bitstreams: 2 S1413-86702007000200012.pdf: 220443 bytes, checksum: 2c23469da3d575381bc17a3d982f0661 (MD5) S1413-86702007000200012.pdf.txt: 40560 bytes, checksum: ea6e3c36f55f0eb685e8ad05b7043fd6 (MD5) Previous issue date: 2007-04-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:34:40Z No. of bitstreams: 2 S1413-86702007000200012.pdf: 220443 bytes, checksum: 2c23469da3d575381bc17a3d982f0661 (MD5) S1413-86702007000200012.pdf.txt: 40560 bytes, checksum: ea6e3c36f55f0eb685e8ad05b7043fd6 (MD5) Made available in DSpace on 2014-05-20T13:34:40Z (GMT). No. of bitstreams: 2 S1413-86702007000200012.pdf: 220443 bytes, checksum: 2c23469da3d575381bc17a3d982f0661 (MD5) S1413-86702007000200012.pdf.txt: 40560 bytes, checksum: ea6e3c36f55f0eb685e8ad05b7043fd6 (MD5) Previous issue date: 2007-04-01 Despite the existence of highly sensitive tests, inconclusive serological results are frequent in chronic chagasic infection. This study aimed to define a diagnostic conduct for 30 individuals with inconclusive serology (G3) for chagasic infection assisted at the Outpatient Unit for Infectious and Parasitic Diseases of the Botucatu School of Medicine. Twenty-one individuals with negative serology (G1) and 33 with positive serology (G2) were also studied. Serological methods ELISA, HAI, IFI and immunoblotting TESA-cruzi were used for G1, G2 and G3, and parasitological methods xenodiagnosis, hemoculture and PCR-LIT were used for G2 and G3 individuals. ELISA, HAI and IFI were performed in 5 different blood samples in G2 and G3. TESA-cruzi was carried out only once in G1, G2 and G3 and, since it is the most sensitive, it was utilized as standard. In G3, positivity for ELISA reached 86% in the fifth blood sample; the ELISA+HAI+IFI combination showed a maximum of 44.8% in the second sample; and TESA-cruzi, 76% in one single sample. Xenodiagnosis positivity was 9.4%; hemoculture showed 15.2%; and PCR-LIT exhibited 22% positivity in G2. Nevertheless, in G3, positivity percentage was 3.4% for xenodiagnosis, 6.7% for PCR-LIT, and no positive result was found for hemoculture. In G3, PCR-LIT resolved one case which was still inconclusive according to serology tests. In order to define inconclusive diagnoses, the results suggest the combined use of ELISA+HAI+IFI in 2 blood samples, decreasing the occurrence of false positive/negative results. If results remain inconclusive, the performance of TESA-cruzi and PCR-LIT, if necessary, is recommended. UNESP Botucatu School of Medicine Department of Tropical Diseases and Imaging Diagnosis UNESP Botucatu School of Medicine Department of Tropical Diseases and Imaging Diagnosis

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T19:05:39Z No. of bitstreams: 1 S0037-86821982000100013.pdf: 149802 bytes, checksum: 8cb2d95c8e86dd49468909fb55fc5eac (MD5) Made available in DSpace on 2013-08-22T19:05:39Z (GMT). No. of bitstreams: 1 S0037-86821982000100013.pdf: 149802 bytes, checksum: 8cb2d95c8e86dd49468909fb55fc5eac (MD5) Previous issue date: 1982-12-01 Made available in DSpace on 2013-09-30T20:09:35Z (GMT). No. of bitstreams: 2 S0037-86821982000100013.pdf: 149802 bytes, checksum: 8cb2d95c8e86dd49468909fb55fc5eac (MD5) S0037-86821982000100013.pdf.txt: 10648 bytes, checksum: b6c9a3298fcc46261020a6a3463c7814 (MD5) Previous issue date: 1982-12-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:17:44Z No. of bitstreams: 2 S0037-86821982000100013.pdf: 149802 bytes, checksum: 8cb2d95c8e86dd49468909fb55fc5eac (MD5) S0037-86821982000100013.pdf.txt: 10648 bytes, checksum: b6c9a3298fcc46261020a6a3463c7814 (MD5) Made available in DSpace on 2014-05-20T15:17:44Z (GMT). No. of bitstreams: 2 S0037-86821982000100013.pdf: 149802 bytes, checksum: 8cb2d95c8e86dd49468909fb55fc5eac (MD5) S0037-86821982000100013.pdf.txt: 10648 bytes, checksum: b6c9a3298fcc46261020a6a3463c7814 (MD5) Previous issue date: 1982-12-01 Universidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Medicina Departamento de Moléstias Infecciosas e Parasitárias, Dermatologia e Radiologia Universidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Medicina Departamento de Moléstias Infecciosas e Parasitárias, Dermatologia e Radiologia

Made available in DSpace on 2022-04-28T16:55:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-01-14. Added 1 bitstream(s) on 2022-04-28T17:10:48Z : No. of bitstreams: 1 S0100-55021980000300053.pdf: 2257901 bytes, checksum: dd3ea4f6d398a609314127319bacf17b (MD5) Faculdade de Medicina de Botucatu, Câmara de Graduação UNESP, IBBMA Faculdade de Medicina de Botucatu UNESP, IBBMA

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T19:05:49Z No. of bitstreams: 1 S0037-86821987000300005.pdf: 325013 bytes, checksum: 3c25efaefe7cf376eb253418ef178fd9 (MD5) Made available in DSpace on 2013-08-22T19:05:49Z (GMT). No. of bitstreams: 1 S0037-86821987000300005.pdf: 325013 bytes, checksum: 3c25efaefe7cf376eb253418ef178fd9 (MD5) Previous issue date: 1987-09-01 Made available in DSpace on 2013-09-30T18:24:55Z (GMT). No. of bitstreams: 1 S0037-86821987000300005.pdf: 325013 bytes, checksum: 3c25efaefe7cf376eb253418ef178fd9 (MD5) Previous issue date: 1987-09-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:38:56Z No. of bitstreams: 1 S0037-86821987000300005.pdf: 325013 bytes, checksum: 3c25efaefe7cf376eb253418ef178fd9 (MD5) Made available in DSpace on 2014-05-20T13:38:56Z (GMT). No. of bitstreams: 1 S0037-86821987000300005.pdf: 325013 bytes, checksum: 3c25efaefe7cf376eb253418ef178fd9 (MD5) Previous issue date: 1987-09-01 Em agosto de 1983 foram observados 85 habitantes do Município de Humaitá, Estado do Amazonas, Brasil, com a finalidade de estudar a prevalência dos antígenos de HLA -A, -B, -C e DR, dentre os quais 38 eram doentes com malária causada pelo Plasmodium falciparum Todos eles foram examinados para avaliação de esplenomegalia, exame parasitológico de sangue e pesquisa de anticorpos de malária. Foram constituídos três grupos: (I) 25 indivíduos nascidos na região Amazônica que nunca tiveram malária; (II) 38 indivíduos naturais da Amazônia que tinham sido tratados de malária no passado, ou que estavam tendo malária atual, e (III) 22 doentes com malária que contraíram na Amazônia e eram procedentes de outras regiões do Brasil. Foram colhidas amostras de sangue de cada um deles, separados os linfôcitos e os antígenos de HLA foram tipados pelo teste de microlinfocitotoxidade. Houve elevada freqüência de antígenos não identificados, nos grupos estudados, o que sugere ou a existência de homozigoze, oufenôtipo não identificado nessa população. Houve alta freqüência fenotípica de antígeno deAg(W24) (44,7%) no Grupo II, quando comparado ao Grupo 1(32%) ou Grupo III (9%). Os indivíduos do Grupo II mostraram também elevada freqüência do antígeno DR4 (80%) quando comparado ao Grupo 1(36,3%) ou Grupo III(16,6%). Essas observações sugerem a possibilidade de suscetibilidadegenética ã malária entre os nativos da Amazônia e indicam a necessidade da realização de inquéritos mais extensos sobre a freqüência de antígenos de HLA em habitantes de zona endêmica de malária. In August 1983,85 inhabitants of the municipality of Humaitá, Amazonas State, Brazil were studied to determine the prevalence of antigens to HLA-A, -B, -C and DR. Thirty-eight were sick with malaria due to Plasmodium falciparum. All subjects were examined for splenomegaly, blood parasitaemia and antibodies to malaria. They constituted three groups: 1) 25 subjects native to the Amazon region who had never had malaria; 2) 38 Amazonian subjects who had malaria in the past or currently had an infection; 3) 22 patients with malaria who had acquired the infection in the Amazon Region but came from other regions of Brazil. Blood was taken from each person, the lymphocytes were separated and typed by the test of microlymphocytotoxicity. There was a high frequency of antigens that could not be identified in the groups studied which suggests the existence of a homozygote or phenotype not identified in the population. There was a high frequency of the phenotype Ag(W24) (44.7%) in group 2 when compared with group 1 (32%) or group 3 (9%). Also the individuals in group 2 showed an elevated frequency of antigen DR(4)80%) when compared with group 1 (36.6%) or group 3 (16.6%). These observations suggest the possibility of a genetic susceptibility to malaria among Amazonian residents and indicate a necessity for more extensive studies of the frequency of HLA antigens among inhabitants of this endemic malarial zone. UNESP School of Medicine Department of Infectious and Parasitic Diseases UNESP School of Medicine Department of Urology Tokai University School of Medicine Transplantation Immunology Center Hospital do Servidor Público do Estado de São Paulo UNESP School of Medicine Department of Infectious and Parasitic Diseases UNESP School of Medicine Department of Urology

Hemoglobin rates, hematocrit and glucose-6-phosphate dehydrogenase (G6PD) and glutathione reductase activities were measured in 38 patients with paracoccidioidomycosis treated with ketoconazole or sulfadoxin, and in 13 normal individuals. Ketoconazole-treated patients showed reduced G6PD and glutathione reductase activities. One of these patients was found to be G6PD-deficient and suffered a hemolytic episode during treatment, which, however, did not require interruption of therapy. The authors suggest that patients showing an erythrocyte enzyme defect should be monitored hematologically during treatment with ketoconazole. They also suggest that ketoconazole is an oxidant drug in addition to being a possible inhibitor of antioxidant erythrocyte enzymes.

A total of 207 patients with malaria caused by Plasmodium falciparum were submitted to 5 different treatment schedules with clindamycin from 1981 to 1984: A - 89 patients were treated intravenously and orally, or intramuscularly and orally with 20 mg/kg/day divided into two daily applications for 5 to 7 days; B-40 patients were treated orally with 20 mg/kg/day divided into two daily doses for 5 to 7 days; C-27 patients were treated with 20 mg/kg/day intravenously or orally divided into two daily applications for 3 days; D-16 patients were treated orally and/or intravenously with a single daily dose of 20 to 40 mg/kg/day for 5 to 7 days; E-35 patients were treated orally with 5 mg/kg/day divided into two doses for 5 days. Patients were examined daily during treatment and reexamined on the 7th, 24th, 21st, 28th and 35th day both clinically and parasitologically (blood test). Eighty three (40.1%) had moderate or severe malaria, and 97 (46.8%) had shown resistance to chloroquine or to the combination ofsulfadoxin and pyrimethamine. The proportion of cured patients was higher than 95% among patients submitted to schedules A and B. Side effects were only occasional and of low intensity. Three deaths occurred (1.4%), two of them involving patients whose signs and symptoms were already very severe when treatment was started. Thus, clindamycin proved to be very useful in the treatment of patients with malaria caused by Plasmodium falciparum and we recommend schedule A for moderate and severe cases and Bfor initial cases.De 1981 a 1984, 207 doentes com malária, causada pelo Plasmodium falciparum, foram tratados com 5 esquemas de clindamicina: A - 89 doente tratados com 20 mg/kg/dia, pelas vias endovenosa e oral, ou intramuscular e oral, em duas aplicações diárias, durante 5 a 7 dias; B - 40 doentes tratados com 20 mg/kg/dia, por via oral, em duas tomadas diárias, durante 5 a 7 dias; C - 27 doentes tratados com 20 mg/kg/dia, por via oral ou endovenosa, em duas tomadas diárias, durante 3 dias; D - 16 doentes tratados com 20 a 40 mg/kg/dia, por vias oral, e/ou, endovenosa, em uma única dose diária, durante 5 a 7 dias; E - 35 doentes tratados com 5 mg/kg/dia, por via oral, em duas doses diárias, durante 5 dias. Os doentes foram examinados, diariamente, durante o tratamento e reexaminados no 7.°, 14.°, 28.° e 35.° dias, tanto pelo exame clínico, quanto pelo parasitológico de sangue. Oitenta e três (40,1%) tinham malária moderada ou grave e 97 (46,8%) tinham apresentado resistência à cloroquina, ou à associação sulfadoxina e pirimetamina. Os resultados mostraram que a proporção de curados foi superior a 95% nos doentes tratados pelos esquemas A e B. Os efeitos colaterais observados foram ocasionais e de pequena intensidade. Houve três casos de óbito (1,4%), dois dos quais atendidos desde o inicio com quadro muito grave. A clindamicina: portanto, mostrou ser muito útil no tratamento de doentes com malária causada pelo Plasmodium falciparum, recomenda-se o esquema A para os casos moderados e o B para os benignos.

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T19:03:44Z No. of bitstreams: 1 S0036-46651985000500001.pdf: 608912 bytes, checksum: 5bcaae1b933a976b0e356929fbef54c0 (MD5) Made available in DSpace on 2013-08-22T19:03:44Z (GMT). No. of bitstreams: 1 S0036-46651985000500001.pdf: 608912 bytes, checksum: 5bcaae1b933a976b0e356929fbef54c0 (MD5) Previous issue date: 1985-10-01 Made available in DSpace on 2013-09-30T18:18:51Z (GMT). No. of bitstreams: 2 S0036-46651985000500001.pdf: 608912 bytes, checksum: 5bcaae1b933a976b0e356929fbef54c0 (MD5) S0036-46651985000500001.pdf.txt: 21906 bytes, checksum: 757c67446082dfae77deed92f28dc78f (MD5) Previous issue date: 1985-10-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:34:16Z No. of bitstreams: 2 S0036-46651985000500001.pdf: 608912 bytes, checksum: 5bcaae1b933a976b0e356929fbef54c0 (MD5) S0036-46651985000500001.pdf.txt: 21906 bytes, checksum: 757c67446082dfae77deed92f28dc78f (MD5) Made available in DSpace on 2014-05-20T13:34:16Z (GMT). No. of bitstreams: 2 S0036-46651985000500001.pdf: 608912 bytes, checksum: 5bcaae1b933a976b0e356929fbef54c0 (MD5) S0036-46651985000500001.pdf.txt: 21906 bytes, checksum: 757c67446082dfae77deed92f28dc78f (MD5) Previous issue date: 1985-10-01 Em agosto de 1983, os Autores, estudaram 36 doentes com infecção causada pelo Plasmodium falciparum e 14 indivíduos normais, nascidos na região de Humaitá, que nunca tiveram malária, sem esplenomegalia, com exame parasitológico de sangue e hemaglutinação passiva negativas. Todos eles foram submetidos à observação clínica completa, exame hematológico, exame parasitológico de sangue e tipagem de linfócitos. Os linfócitos foram isolados e congelados em nitrogênio líquido, para posterior tipagem pela formação de rosetas. Os doentes foram classificados em dois grupos de acordo com a presença (13 doentes), ou ausência (23 doentes) de gametócitos antes do tratamento. Houve predomínio de formas graves no grupo de doentes sem gametócitos. Os resultados mostraram diminuição do número de linfócitos T em ambos os grupos de doentes, com ou sem gametócitos antes do tratamento, enquanto que o número de linfócitos B foi normal apenas no grupo de doentes com gametócitos. Essas observações, assim como as que foram relatadas pelos Autores anteriormente, permitem associar a presença de gametócitos circulantes com o número normal de células B em doentes com formas leves de malária causada pelo Plasmodium falciparum. In August 1983 the Authors studied 36 patients with Plasmodium falciparum malaria and 14 normal individuals born in Humaita region who had never had malaria, had no spleen enlargement and had negative parasitemia as well as passive hemagglutination. Medical histories were obtained and complete physical examination were performed in all of them just as blood tests, parasite density and lymphocyte typing. The lymphocytes were separated and then frozen in liquid nitrogen for later typing by rosette formation. The patients were divided in two groups according to the presence (13 patients) or abscence (23 patients) of gametocytes before treatment. Severe malaria was predominant in the group without gametocytes. The results showed a decrease in the T-cell numbers in Plasmodium falciparum acute malaria patients both with or without gametocytes before the treatment, while B-cell numbers were normal only in the patients with gametocytes. These observations as like as those previously reported by the Authors, permit to associate the presence of gametocytes in peripheral blood and normal number of B-cells in patients with mild Plasmodium falciparum malaria. UNESP School of Medicine Botucatu Department of Infectious and Parasitologic Diseases, Dermatology and Radiology UNESP School of Medicine Botucatu Department of Surgery São Paulo State Public Functionary Hospital São Paulo State Public Functionary Hospital Department of Blood Bank UNESP Basic Institute of Medical and Agricultural Biology Department of Microbiology and Immunology UNESP School of Medicine Botucatu Department of Infectious and Parasitologic Diseases, Dermatology and Radiology UNESP School of Medicine Botucatu Department of Surgery UNESP Basic Institute of Medical and Agricultural Biology Department of Microbiology and Immunology

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T19:03:59Z No. of bitstreams: 1 S0036-46651988000500010.pdf: 401601 bytes, checksum: 9cf04531b0af31ba123b3d3b180ab9cc (MD5) Made available in DSpace on 2013-08-22T19:03:59Z (GMT). No. of bitstreams: 1 S0036-46651988000500010.pdf: 401601 bytes, checksum: 9cf04531b0af31ba123b3d3b180ab9cc (MD5) Previous issue date: 1988-10-01 Made available in DSpace on 2013-09-30T20:06:31Z (GMT). No. of bitstreams: 2 S0036-46651988000500010.pdf: 401601 bytes, checksum: 9cf04531b0af31ba123b3d3b180ab9cc (MD5) S0036-46651988000500010.pdf.txt: 11866 bytes, checksum: 3120b4bb760218d1fe810a91055ac5d3 (MD5) Previous issue date: 1988-10-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:36:03Z No. of bitstreams: 2 S0036-46651988000500010.pdf: 401601 bytes, checksum: 9cf04531b0af31ba123b3d3b180ab9cc (MD5) S0036-46651988000500010.pdf.txt: 11866 bytes, checksum: 3120b4bb760218d1fe810a91055ac5d3 (MD5) Made available in DSpace on 2014-05-20T13:36:03Z (GMT). No. of bitstreams: 2 S0036-46651988000500010.pdf: 401601 bytes, checksum: 9cf04531b0af31ba123b3d3b180ab9cc (MD5) S0036-46651988000500010.pdf.txt: 11866 bytes, checksum: 3120b4bb760218d1fe810a91055ac5d3 (MD5) Previous issue date: 1988-10-01 UNESP Faculty of Medicine of Botucatu Department of Neurology and Psychiatry UNESP Faculty of Medicine of Botucatu Department of Infectious and Parasitic Diseases UNESP) Faculty of Medicine of Botucatu Department of Ophthalmology and Otorhinolaryngology UNESP Faculty of Medicine of Botucatu UNESP Faculty of Medicine of Botucatu Department of Neurology and Psychiatry UNESP Faculty of Medicine of Botucatu Department of Infectious and Parasitic Diseases UNESP) Faculty of Medicine of Botucatu Department of Ophthalmology and Otorhinolaryngology UNESP Faculty of Medicine of Botucatu

Made available in DSpace on 2021-06-25T10:56:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Background: Despite the many benefits that follow antiretroviral therapy (ART) initiation, its chronic use contributes to the early aging of people living with HIV/AIDS (PLWHA). The aim of this cross-sectional study was to trace the prevalence of and investigate possible renal, bone and metabolic changes, as well as cardiovascular risk in 94 asymptomatic PLWHA, relating them to the duration of ART use. Methods: Four groups were evaluated according to ART use: G1 (n = 21), ART-naïve individuals; G2 (n = 17)