51. Ionizing radiation promotes CCL27 secretion from keratinocytes through the cross talk between TNF-α and ROS.
- Author
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Zhang Q, Zhu L, Wang G, Zhao Y, Xiong N, Bao H, and Jin W
- Subjects
- Dermatitis, Contact immunology, Dermatitis, Contact metabolism, Dermatitis, Contact pathology, Humans, Inflammation immunology, Inflammation pathology, Keratinocytes metabolism, Keratinocytes radiation effects, NF-kappa B metabolism, Psoriasis immunology, Psoriasis metabolism, Psoriasis pathology, Radiation, Ionizing, Receptors, CCR10 metabolism, T-Lymphocytes immunology, T-Lymphocytes radiation effects, Chemokine CCL27 metabolism, Immunity, Cellular drug effects, Inflammation metabolism, Reactive Oxygen Species metabolism, Tumor Necrosis Factor-alpha metabolism
- Abstract
The skin-associated chemokine CCL27 and its receptor CCR10 mediate the immune response of skin-homing T cells. The CCL27 secreted from keratinocytes was reportedly involved in inflammatory skin diseases such as atopic dermatitis, contact dermatitis, and psoriasis. However, whether ionizing radiation increases the levels of CCL27 secretion still remains unclear. In HaCaT cells, a human keratinocyte cell line, CCL27 secretion was markedly increased after X-ray irradiation. We further found that irradiation boosted the generation of reactive oxygen species (ROS), which was concomitant with the release of tumor necrosis factor-alpha (TNF-α). Moreover, alteration of ROS in irradiated HaCaT cells correlated with TNF-α secretion, indicating a positive loop of TNF-α secretion and ROS generation. This positive loop regulated the secretion of CCL27 from irradiated cells. We therefore concluded that the cross talk between TNF-α and ROS after keratinocytes was exposed to radiation, triggered CCL27 secretion for subsequent inflammation response., (© 2016 Wiley Periodicals, Inc.)
- Published
- 2017
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