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Anti-Inflammatory and Antioxidant Effects of Repeated Exposure to Cruciferous Allyl Nitrile in Sensitizer-Induced Ear Edema in Mice.
- Source :
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Medical science monitor basic research [Med Sci Monit Basic Res] 2016 Feb 29; Vol. 22, pp. 20-6. Date of Electronic Publication: 2016 Feb 29. - Publication Year :
- 2016
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Abstract
- Background: Skin sensitizers induce allergic reactions through the induction of reactive oxygen species. Allyl nitrile from cruciferous vegetables has been reported to induce antioxidants and phase II detoxification enzymes in various tissues. We assessed the effects of repeated exposure to allyl nitrile on sensitizer-induced allergic reactions.<br />Material and Methods: Mice were dosed with allyl nitrile (0-200 µmol/kg), and then received a dermal application of 1 of 3 sensitizers on the left ear or 1 of 2 vehicles on the right ear. Quantitative assessment of edema was carried out by measuring the difference in weight between the portions taken from the right and left ears. We tested enzymes (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and thiobarbituric acid reactive substances (TBARS) in ears.<br />Results: Repeated exposure to allyl nitrile reduced edemas induced by glutaraldehyde and by 2, 4-dinitrochlorobenzene (DNCB), but not by formaldehyde. The repeated exposure decreased levels of TBARS, a marker of oxidative stress, induced by glutaraldehyde and by DNCB, but not by formaldehyde. Allyl nitrile elevated SOD levels for the 3 sensitizers, and CAT levels for formaldehyde and DNCB. Allyl nitrile also increased GPx levels for formaldehyde and DNCB, but not for glutaraldehyde. The reduced edemas were associated with changes in oxidative stress levels and antioxidant enzymes.<br />Conclusions: Repeated exposure to allyl nitrile reduced allergic reactions induced by glutaraldehyde and by DNCB, but not by formaldehyde. This reduction was associated with changes in ROS levels and antioxidant enzyme activities.
- Subjects :
- Animals
Catalase metabolism
Dermatitis, Contact metabolism
Ear
Edema chemically induced
Edema prevention & control
Glutathione Peroxidase metabolism
Male
Mice
Oxidative Stress drug effects
Reactive Oxygen Species metabolism
Superoxide Dismutase metabolism
Thiobarbituric Acid Reactive Substances metabolism
Allyl Compounds pharmacology
Anti-Inflammatory Agents pharmacology
Antioxidants pharmacology
Dermatitis, Contact prevention & control
Nitriles pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 2325-4416
- Volume :
- 22
- Database :
- MEDLINE
- Journal :
- Medical science monitor basic research
- Publication Type :
- Academic Journal
- Accession number :
- 26932717
- Full Text :
- https://doi.org/10.12659/MSMBR.897771