51. Layered acoustofluidic resonators for the simultaneous optical and acoustic characterisation of cavitation dynamics, microstreaming, and biological effects
- Author
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Eleanor Stride, Oliver Vince, Miles Aron, Michel Versluis, Guillaume Lajoinie, Anjali Seth, Constantin C. Coussios, M. de Saint Victor, Christophoros Mannaris, Dario Carugo, Valerio Pereno, and Physics of Fluids
- Subjects
0301 basic medicine ,Fluid Flow and Transfer Processes ,Materials science ,business.industry ,Acoustics ,Microfluidics ,Dynamics (mechanics) ,Ultrasound ,Biomedical Engineering ,Ultrasound exposure ,02 engineering and technology ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,03 medical and health sciences ,Resonator ,030104 developmental biology ,Colloid and Surface Chemistry ,Cavitation ,Microscopy ,Microbubbles ,General Materials Science ,0210 nano-technology ,business ,Regular Articles - Abstract
The study of the effects of ultrasound-induced acoustic cavitation on biological structures is an active field in biomedical research. Of particular interest for therapeutic applications is the ability of oscillating microbubbles to promote both cellular and tissue membrane permeabilisation and to improve the distribution of therapeutic agents in tissue through extravasation and convective transport. The mechanisms that underpin the interaction between cavitating agents and tissues are, however, still poorly understood. One challenge is the practical difficulty involved in performing optical microscopy and acoustic emissions monitoring simultaneously in a biologically compatible environment. Here we present and characterise a microfluidic layered acoustic resonator (μLAR) developed for simultaneous ultrasound exposure, acoustic emissions monitoring, and microscopy of biological samples. The μLAR facilitates in vitro ultrasound experiments in which measurements of microbubble dynamics, microstreaming velocity fields, acoustic emissions, and cell-microbubble interactions can be performed simultaneously. The device and analyses presented provide a means of performing mechanistic in vitro studies that may benefit the design of predictable and effective cavitation-based ultrasound treatments.
- Published
- 2018