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Giant vesicles as cell models to quantify bio-effects in ultrasound mediated drug delivery
- Source :
- The Journal of the Acoustical Society of America. 140:3025-3025
- Publication Year :
- 2016
- Publisher :
- Acoustical Society of America (ASA), 2016.
-
Abstract
- The biophysical mechanisms that underpin the interaction between microbubbles and cells in the context of ultrasound-mediated drug delivery are still poorly understood. To aid the identification of these mechanisms, giant unilamellar vesicles (GUVs) were used as cell models to quantify changes in membrane properties as a result of the interaction with ultrasound (1 MHz, 150 kPa, and 60 s continuous wave) and phospholipid-shelled microbubbles (DSPC-PEG40S 9:1 molar ratio), either alone or in combination. The spatial quantification of the vesicle lipid order was performed via spectral microscope imaging, by measuring the contours of generalized polarisation (GP) from the emission spectrum of c-Laurdan, a polarity-sensitive dye. Preliminary data show synergistic mechanical and chemical effects on membranes: ultrasound exposure and shear flow alone generally decrease the vesicle lipid packing, while exposures involving microbubbles reveal contrasting effects depending on the initial vesicle composition and ac...
- Subjects :
- Acoustics and Ultrasonics
business.industry
Chemistry
Vesicle
Cell
Ultrasound
Context (language use)
Nanotechnology
medicine.anatomical_structure
Membrane
Arts and Humanities (miscellaneous)
Giant vesicles
Drug delivery
medicine
Biophysics
Microbubbles
lipids (amino acids, peptides, and proteins)
business
Subjects
Details
- ISSN :
- 00014966
- Volume :
- 140
- Database :
- OpenAIRE
- Journal :
- The Journal of the Acoustical Society of America
- Accession number :
- edsair.doi...........81632dad8b5f018f9527ca92a8e8517c