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56. A Heparan Sulfate Device for the Regeneration of Osteochondral Defects

Author

Lee, Jonathan H., primary, Luo, Xiaoman, additional, Ren, Xiafei, additional, Tan, Tuan Chun, additional, Smith, Raymond A.A., additional, Swaminathan, Kunchithapadam, additional, Sekar, Sakthivel, additional, Bhakoo, Kishore, additional, Nurcombe, Victor, additional, Hui, James H., additional, and Cool, Simon M., additional

Published
2019
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58. Telomere length analysis of human mesenchymal stem cells by quantitative PCR

Author

Samsonraj, Rebekah M, Raghunath, Michael, Hui, James H, Ling, Ling, Nurcombe, Victor, Cool, Simon M, Samsonraj, Rebekah M, Raghunath, Michael, Hui, James H, Ling, Ling, Nurcombe, Victor, and Cool, Simon M

Abstract

Human mesenchymal stem cells (hMSCs) have attracted much attention for tissue repair and wound healing because of their self-renewal capacity and multipotentiality. In order to mediate an effective therapy, substantial numbers of cells are required, which necessitates extensive sub-culturing and expansion of hMSCs. Throughout ex vivo expansion, the cells undergo telomere shortening, and critically short telomeres can trigger loss of cell viability. Telomeres are nucleoprotein structures that cap the ends of chromosomes, and serve to protect the DNA from the degradation which occurs due to the end-replication problem in all eukaryotes. As hMSCs have only a finite ability for self-renewal like most somatic cells, assaying for telomere length in hMSCs provides critical information on the replicative capacity of the cells, an important criterion in the selection of hMSCs for therapy. Telomere length is generally quantified by Southern blotting and fluorescence in situ hybridization, and more recently by PCR-based methods. Here we describe the quantification of hMSC telomere length by real-time PCR; our results demonstrate the effect of telomere shortening on the proliferation and clonogenicity of hMSCs. Thus, this assay constitutes a useful tool for the determination of relative telomere length in hMSCs.

Published
2018

59. Establishing criteria for human mesenchymal stem cell potency

Author

Samsonraj, Rebekah M., Rai, Bina, Sathiyanathan, Padmapriya, Puan, Kia Joo, Rötzschke, Olaf, Hui, James H., Raghunath, Michael, Stanton, Lawrence W., Nurcombe, Victor, Cool, Simon M., Samsonraj, Rebekah M., Rai, Bina, Sathiyanathan, Padmapriya, Puan, Kia Joo, Rötzschke, Olaf, Hui, James H., Raghunath, Michael, Stanton, Lawrence W., Nurcombe, Victor, and Cool, Simon M.

Abstract

This study sought to identify critical determinants of mesenchymal stem cell (MSC) potency using in vitro and in vivo attributes of cells isolated from the bone marrow of age‐ and sex‐matched donors. Adherence to plastic was not indicative of potency, yet capacity for long‐term expansion in vitro varied considerably between donors, allowing the grouping of MSCs from the donors into either those with high‐growth capacity or low‐growth capacity. Using this grouping strategy, high‐growth capacity MSCs were smaller in size, had greater colony‐forming efficiency, and had longer telomeres. Cell‐surface biomarker analysis revealed that the International Society for Cellular Therapy (ISCT) criteria did not distinguish between high‐growth capacity and low‐growth capacity MSCs, whereas STRO‐1 and platelet‐derived growth factor receptor alpha were preferentially expressed on high‐growth capacity MSCs. These cells also had the highest mean expression of the mRNA transcripts TWIST‐1 and DERMO‐1. Irrespective of these differences, both groups of donor MSCs produced similar levels of key growth factors and cytokines involved in tissue regeneration and were capable of multilineage differentiation. However, high‐growth capacity MSCs produced approximately double the volume of mineralized tissue compared to low‐growth capacity MSCs when assessed for ectopic bone‐forming ability. The additional phenotypic criteria presented in this study when combined with the existing ISCT minimum criteria and working proposal will permit an improved assessment of MSC potency and provide a basis for establishing the quality of MSCs prior to their therapeutic application.

Published
2018

61. Validation of Osteogenic Properties of Cytochalasin D by High-Resolution RNA-Sequencing in Mesenchymal Stem Cells Derived from Bone Marrow and Adipose Tissues

Author

Samsonraj, Rebekah M., primary, Paradise, Christopher R., additional, Dudakovic, Amel, additional, Sen, Buer, additional, Nair, Asha A., additional, Dietz, Allan B., additional, Deyle, David R., additional, Cool, Simon M., additional, Rubin, Janet, additional, and van Wijnen, Andre J., additional

Published
2018
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64. Extracellular matrix protein production in human adipose-derived mesenchymal stem cells on three-dimensional polycaprolactone (PCL) scaffolds responds to GDF5 or FGF2

Author

Su, Yan, primary, Denbeigh, Janet M., additional, Camilleri, Emily T., additional, Riester, Scott M., additional, Parry, Joshua A., additional, Wagner, Eric R., additional, Yaszemski, Michael J., additional, Dietz, Allan B., additional, Cool, Simon M., additional, van Wijnen, Andre J., additional, and Kakar, Sanjeev, additional

Published
2018
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67. Immobilization of vitronectin‐binding heparan sulfates onto surfaces to support human pluripotent stem cells

Author

Yap, Lynn, primary, Murali, Sadasivam, additional, Bhakta, Gajadhar, additional, Titmarsh, Drew M., additional, Chen, Allen Kuan‐Liang, additional, Chiin Sim, Lyn, additional, Bardor, Muriel, additional, Lim, Yu Ming, additional, Goh, James C.H., additional, Oh, Steve K.W., additional, Choo, Andre B.H., additional, van Wijnen, Andre J., additional, Robinson, David E., additional, Whittle, Jason D., additional, Birch, William R., additional, Short, Robert D., additional, Nurcombe, Victor, additional, and Cool, Simon M., additional

Published
2017
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68. Long-Term Culture of Self-renewing Pancreatic Progenitors Derived from Human Pluripotent Stem Cells

Author

Trott, Jamie, primary, Tan, Ee Kim, additional, Ong, Sheena, additional, Titmarsh, Drew M., additional, Denil, Simon L.I.J., additional, Giam, Maybelline, additional, Wong, Cheng Kit, additional, Wang, Jiaxu, additional, Shboul, Mohammad, additional, Eio, Michelle, additional, Cooper-White, Justin, additional, Cool, Simon M., additional, Rancati, Giulia, additional, Stanton, Lawrence W., additional, Reversade, Bruno, additional, and Dunn, N. Ray, additional

Published
2017
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71. A genomic biomarker that identifies human bone marrow‐derivedmesenchymal stem cells with high scalability

Author

Sathiyanathan, Padmapriya, Samsonraj, Rebekah M., Tan, Clarissa L. L., Ling, Ling, Lezhava, Alexander, Nurcombe, Victor, Stanton, Lawrence W., and Cool, Simon M.

Abstract

Although the application of human mesenchymal stem cells (hMSCs) to repair damaged or diseased tissues has proven relatively effective, both the donor‐to‐donor variability in ex vivo expansion rates and the maintenance of stemness remain a bottleneck to widespread translation. Previous work from this laboratory stratified donors into those yielding hMSCs with high‐ or low‐growth capacity; global transcriptomic analysis revealed that high‐growth‐capacity hMSCs were characterized by a loss of the gene encoding glutathione S‐transferase theta 1 (GSTT1). These GSTT1‐null hMSCs demonstrated increased proliferative rates, clonogenic potential, and longer telomeres compared with low‐growth capacity hMSCs that were GSTT1‐positive. Thus, this study identifies GSTT1as a novel genomic DNA biomarker for hMSC scalability. GSTT1is a novel genomic biomarker of human mesenchymal stem cell (hMSC) scalability. GSTT1‐null (GSTT1*0/*0) hMSCs possess higher clonogenicity and proliferative capacity in comparison to homozygous positive (GSTT1*1/*1) cells.

Published
2020
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72. Heparan sulfate proteoglycans and human breast cancer epithelial cell tumorigenicity

Author

Okolicsanyi, Rachel K., van Wijnen, Andre J., Cool, Simon M., Stein, Gary S., Griffiths, Lyn R., and Haupt, Larisa M.

Subjects
HEPARAN SULFATE PROTEOGLYCANS, MCF‐7, GENE EXPRESSION, BREAST CANCER, Wnt SIGNALING, MDA‐MB‐231, 060103 Cell Development Proliferation and Death
Abstract

Heparan sulfate proteoglycans (HSPGs) are key components of the extracellular matrix that mediate cell proliferation, invasion, and cellular signaling. The biological functions of HSPGs are linked to their co-stimulatory effects on extracellular ligands (e.g., WNTs) and the resulting activation of transcription factors that control mammalian development but also associated with tumorigenesis. We examined the expression profile of HSPG core protein syndecans (SDC1–4) and glypicans (GPC1–6) along with the enzymes that initiate or modify their glycosaminoglycan chains in human breast cancer (HBC) epithelial cells. Gene expression in relation to cell proliferation was examined in the HBC cell lines MCF-7 and MDA-MB-231 following treatment with the HS agonist heparin. Heparin increased gene expression of chain initiation and modification enzymes including EXT1 and NDST1, as well as core proteins SDC2 and GPC6. With HS/Wnt interactions established, we next investigated WNT pathway components and observed that increased proliferation of the more invasive MDA-MB-231 cells is associated with activation of the Wnt signaling pathway. Specifically, there was substantial upregulation (>5-fold) of AXIN1, WNT4A, and MYC in MDA-MB-231 but not in MCF-7 cells. The changes in gene expression observed for HSPG core proteins and related enzymes along with the associated Wnt signaling components suggest coordinated interactions. The influence of HSPGs on cellular proliferation and invasive potential of breast cancer epithelial cells are cell and niche specific. Further studies on the interactions between HSPGs and WNT ligands may yield clinically relevant molecular targets, as well as new biomarkers for characterization of breast cancer progression.

Published
2014

74. The synovial microenvironment of osteoarthritic joints alters RNA-seq expression profiles of human primary articular chondrocytes

Author

Lewallen, Eric A., primary, Bonin, Carolina A., additional, Li, Xin, additional, Smith, Jay, additional, Karperien, Marcel, additional, Larson, A. Noelle, additional, Lewallen, David G., additional, Cool, Simon M., additional, Westendorf, Jennifer J., additional, Krych, Aaron J., additional, Leontovich, Alexey A., additional, Im, Hee-Jeong, additional, and van Wijnen, Andre J., additional

Published
2016
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75. Affinity Selection of FGF2-Binding Heparan Sulfates for Ex Vivo Expansion of Human Mesenchymal Stem Cells

Author

Wijesinghe, Sampath Jeewantha, primary, Ling, Ling, additional, Murali, Sadasivam, additional, Qing, Yeong Hui, additional, Hinkley, Simon F.R., additional, Carnachan, Susan M., additional, Bell, Tracey J., additional, Swaminathan, Kunchithapadam, additional, Hui, James H., additional, van Wijnen, Andre J., additional, Nurcombe, Victor, additional, and Cool, Simon M., additional

Published
2016
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76. Effect of heparin on the biological properties and molecular signature of human mesenchymal stem cells

Author

Ling, Ling, primary, Camilleri, Emily T., additional, Helledie, Torben, additional, Samsonraj, Rebekah M., additional, Titmarsh, Drew M., additional, Chua, Ren Jie, additional, Dreesen, Oliver, additional, Dombrowski, Christian, additional, Rider, David A., additional, Galindo, Mario, additional, Lee, Ian, additional, Hong, Wanjin, additional, Hui, James H., additional, Nurcombe, Victor, additional, van Wijnen, Andre J., additional, and Cool, Simon M., additional

Published
2016
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78. Immobilization of vitronectin‐binding heparan sulfates onto surfaces to support human pluripotent stem cells.

Author

Yap, Lynn, Murali, Sadasivam, Bhakta, Gajadhar, Titmarsh, Drew M., Chen, Allen Kuan‐Liang, Chiin Sim, Lyn, Bardor, Muriel, Lim, Yu Ming, Goh, James C. H., Oh, Steve K. W., Choo, Andre B. H., van Wijnen, Andre J., Robinson, David E., Whittle, Jason D., Birch, William R., Short, Robert D., Nurcombe, Victor, and Cool, Simon M.

Abstract

Abstract: Functionalizing medical devices with polypeptides to enhance their performance has become important for improved clinical success. The extracellular matrix (ECM) adhesion protein vitronectin (VN) is an effective coating, although the chemistry used to attach VN often reduces its bioactivity. In vivo, VN binds the ECM in a sequence‐dependent manner with heparan sulfate (HS) glycosaminoglycans. We reasoned therefore that sequence‐based affinity chromatography could be used to isolate a VN‐binding HS fraction (HS9) for use as a coating material to capture VN onto implant surfaces. Binding avidity and specificity of HS9 were confirmed by enzyme‐linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR)‐based assays. Plasma polymerization of allylamine (AA) to tissue culture‐treated polystyrene (TCPS) was then used to capture and present HS9 as determined by radiolabeling and ELISA. HS9‐coated TCPS avidly bound VN, and this layered surface supported the robust attachment, expansion, and maintenance of human pluripotent stem cells. Compositional analysis demonstrated that 6‐O‐ and N‐sulfation, as well as lengths greater than three disaccharide units (dp6) are critical for VN binding to HS‐coated surfaces. Importantly, HS9 coating reduced the threshold concentration of VN required to create an optimally bioactive surface for pluripotent stem cells. We conclude that affinity‐purified heparan sugars are able to coat materials to efficiently bind adhesive factors for biomedical applications. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1887–1896, 2018. [ABSTRACT FROM AUTHOR]

Published
2018
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81. Polyelectrolyte Complex Carrier Enhances Therapeutic Efficiency and Safety Profile of Bone Morphogenetic Protein-2 in Porcine Lumbar Interbody Fusion Model

Author

Wang, Ming, primary, Abbah, Sunny Akogwu, additional, Hu, Tao, additional, Lam, Raymond Wing Moon, additional, Toh, Soo Yein, additional, Liu, Tong, additional, Cool, Simon M., additional, Bhakoo, Kishore, additional, Li, Jun, additional, Goh, James Cho Hong, additional, and Wong, Hee-Kit, additional

Published
2015
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82. Establishing Criteria for Human Mesenchymal Stem Cell Potency

Author

Samsonraj, Rebekah M., primary, Rai, Bina, additional, Sathiyanathan, Padmapriya, additional, Puan, Kia Joo, additional, Rötzschke, Olaf, additional, Hui, James H., additional, Raghunath, Michael, additional, Stanton, Lawrence W., additional, Nurcombe, Victor, additional, and Cool, Simon M., additional

Published
2015
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83. Novel Protamine-Based Polyelectrolyte Carrier Enhances Low-Dose rhBMP-2 in Posterolateral Spinal Fusion

Author

Hu, Tao, primary, Abbah, Sunny Akogwu, additional, Wang, Ming, additional, Toh, Soo Yein, additional, Lam, Raymond Wing Moon, additional, Naidu, Mathanapriya, additional, Bhakta, Gajadhar, additional, Cool, Simon M., additional, Bhakoo, Kishore, additional, Li, Jun, additional, Goh, James Cho-Hong, additional, and Wong, Hee-Kit, additional

Published
2015
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85. Affinity Selection of FGF2-Binding Heparan Sulfates for Ex Vivo Expansion of Human Mesenchymal Stem Cells.

Author

Wijesinghe, Sampath Jeewantha, Ling, Ling, Murali, Sadasivam, Qing, Yeong Hui, Hinkley, Simon F.R., Carnachan, Susan M., Bell, Tracey J., Swaminathan, Kunchithapadam, Hui, James H., van Wijnen, Andre J., Nurcombe, Victor, and Cool, Simon M.

Subjects
MESENCHYMAL stem cells, CELLULAR therapy, FIBROBLAST growth factors, HEPARAN sulfate, CHROMATOGRAPHIC analysis
Abstract

The future of human mesenchymal stem cells (hMSCs) as a successful cell therapy relies on bioprocessing strategies to improve the scalability of these cells without compromising their therapeutic ability. The culture-expansion of hMSCs can be enhanced by supplementation with growth factors, particularly fibroblast growth factor 2 (FGF2). The biological activity of FGF2 is controlled through interactions with heparan sulfate (HS) that facilitates ligand-receptor complex formation. We previously reported on an FGF2-interacting HS variant (termed HS2) isolated from embryonic tissue by anionic exchange chromatography that increased the proliferation and potency of hMSCs. Here, we detail the isolation of an FGF2 affinity-purified HS variant (HS8) using a scalable platform technology previously employed to generate HS variants with increased affinity for BMP-2 or VEGF165. This process used a peptide sequence derived from the heparin-binding domain of FGF2 as a substrate to affinity-isolate HS8 from a commercially available source of porcine mucosal HS. Our data show that HS8 binds to FGF2 with higher affinity than to FGF1, FGF7, BMP2, PDGF-BB, or VEGF165. Also, HS8 protects FGF2 from thermal destabilization and increases FGF signaling and hMSC proliferation through FGF receptor 1. Long-term supplementation of cultures with HS8 increased both hMSC numbers and their colony-forming efficiency without adversely affecting the expression of hMSC-related cell surface antigens. This strategy further exemplifies the utility of affinity-purifying HS variants against particular ligands important to the stem cell microenvironment and advocates for their addition as adjuvants for the culture-expansion of hMSCs destined for cellular therapy. J. Cell. Physiol. 232: 566-575, 2017. © 2016 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]

Published
2017
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86. High-Resolution Molecular Validation of Self-Renewal and Spontaneous Differentiation in Clinical-Grade Adipose-Tissue Derived Human Mesenchymal Stem Cells

Author

Dudakovic, Amel, primary, Camilleri, Emily, additional, Riester, Scott M., additional, Lewallen, Eric A., additional, Kvasha, Sergiy, additional, Chen, Xiaoyue, additional, Radel, Darcie J., additional, Anderson, Jarett M., additional, Nair, Asha A., additional, Evans, Jared M., additional, Krych, Aaron J., additional, Smith, Jay, additional, Deyle, David R., additional, Stein, Janet L., additional, Stein, Gary S., additional, Im, Hee-Jeong, additional, Cool, Simon M., additional, Westendorf, Jennifer J., additional, Kakar, Sanjeev, additional, Dietz, Allan B., additional, and van Wijnen, Andre J., additional

Published
2014
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89. Complexation and sequestration of BMP-2 from an ECM mimetic hyaluronan gel for improved bone formation

Author

Kisiel, Marta, Klar, Agnieszka S, Ventura, Manuela, Buijs, Jos, Mafina, Marc-Krystelle, Cool, Simon M, Hilborn, Jöns, Kisiel, Marta, Klar, Agnieszka S, Ventura, Manuela, Buijs, Jos, Mafina, Marc-Krystelle, Cool, Simon M, and Hilborn, Jöns

Abstract

Bone morphogenetic protein-2 (BMP-2) is considered a promising adjuvant for the treatment of skeletal non-union and spinal fusion. However, BMP-2 delivery in a conventional collagen scaffold necessitates a high dose to achieve an efficacious outcome. To lower its effective dose, we precomplexed BMP-2 with the glycosaminoglycans (GAGs) dermatan sulfate (DS) or heparin (HP), prior to loading it into a hyaluronic acid (HA) hydrogel. In vitro release studies showed that BMP-2 precomplexed with DS or HP had a prolonged delivery compared to without GAG. BMP-2-DS complexes achieved a slightly faster release in the first 24 h than HP; however, both delivered BMP-2 for an equal duration. Analysis of the kinetic interaction between BMP-2 and DS or HP showed that HP had approximately 10 times higher affinity for BMP-2 than DS, yet it equally stabilized the protein, as determined by alkaline phosphatase activity. Ectopic bone formation assays at subcutaneous sites in rats demonstrated that HA hydrogel-delivered BMP-2 precomplexed with GAG induced twice the volume of bone compared with BMP-2 delivered uncomplexed to GAG.

Published
2013

90. FGFR1 Signaling Stimulates Proliferation of Human Mesenchymal Stem Cells by Inhibiting the Cyclin-Dependent Kinase Inhibitors p21Waf1 and p27Kip1

Author

Dombrowski, Christian, primary, Helledie, Torben, additional, Ling, Ling, additional, Grünert, Martin, additional, Canning, Claire A., additional, Jones, C. Michael, additional, Hui, James H., additional, Nurcombe, Victor, additional, van Wijnen, Andre J., additional, and Cool, Simon M., additional

Published
2013
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92. MicroRNA-34c Inversely Couples the Biological Functions of the Runt-related Transcription Factor RUNX2 and the Tumor Suppressor p53 in Osteosarcoma

Author

van der Deen, Margaretha, primary, Taipaleenmäki, Hanna, additional, Zhang, Ying, additional, Teplyuk, Nadiya M., additional, Gupta, Anurag, additional, Cinghu, Senthilkumar, additional, Shogren, Kristen, additional, Maran, Avudaiappan, additional, Yaszemski, Michael J., additional, Ling, Ling, additional, Cool, Simon M., additional, Leong, David T., additional, Dierkes, Christian, additional, Zustin, Jozef, additional, Salto-Tellez, Manuel, additional, Ito, Yoshiaki, additional, Bae, Suk-Chul, additional, Zielenska, Maria, additional, Squire, Jeremy A., additional, Lian, Jane B., additional, Stein, Janet L., additional, Zambetti, Gerard P., additional, Jones, Stephen N., additional, Galindo, Mario, additional, Hesse, Eric, additional, Stein, Gary S., additional, and van Wijnen, Andre J., additional

Published
2013
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95. Heparan Sulfate Enhances the Self-Renewal and Therapeutic Potential of Mesenchymal Stem Cells from Human Adult Bone Marrow

Author

Helledie, Torben, primary, Dombrowski, Christian, additional, Rai, Bina, additional, Lim, Zophia X.H., additional, Hin, Ian Lee Hock, additional, Rider, David A., additional, Stein, Gary S., additional, Hong, Wanjin, additional, van Wijnen, Andre J., additional, Hui, James H., additional, Nurcombe, Victor, additional, and Cool, Simon M., additional

Published
2012
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97. Functional maturation of isolated neural progenitor cells from the adult rat hippocampus

Author

Hogg, Ron C, Chipperfield, Hiram, Whyte, K A, Stafford, Mark R, Hansen, Mitchell A, Cool, Simon M, Nurcombe, Victor, Adams, David J, Hogg, Ron C, Chipperfield, Hiram, Whyte, K A, Stafford, Mark R, Hansen, Mitchell A, Cool, Simon M, Nurcombe, Victor, and Adams, David J

Abstract

Although neural progenitor cells (NPCs) may provide a source of new neurons to alleviate neural trauma, little is known about their electrical properties as they differentiate. We have previously shown that single NPCs from the adult rat hippocampus can be cloned in the presence of heparan sulphate chains purified from the hippocampus, and that these cells can be pushed into a proliferative phenotype with the mitogen FGF2 [Chipperfield, H., Bedi, K.S., Cool, S.M. & Nurcombe, V. (2002) Int. J. Dev. Biol., 46, 661-670]. In this study, the active and passive electrical properties of both undifferentiated and differentiated adult hippocampal NPCs, from 0 to 12 days in vitro as single-cell preparations, were investigated. Sparsely plated, undifferentiated NPCs had a resting membrane potential of ≈ -90 mV and were electrically inexcitable. In >70%, ATP and benzoylbenzoyl-ATP evoked an inward current and membrane depolarization, whereas acetylcholine, noradrenaline, glutamate and GABA had no detectable effect. In Fura-2-loaded undifferentiated NPCs, ATP and benzoylbenzoyl-ATP evoked a transient increase in the intracellular free Ca 2+ concentration, which was dependent on extracellular Ca 2+ and was inhibited reversibly by pyridoxalphosphate-6-azophenyl- 2′-4′-disulphonic acid (PPADS), a P2 receptor antagonist. After differentiation, NPC-derived neurons became electrically excitable, expressing voltage-dependent TTX-sensitive Na+ channels, low- and high-voltage-activated Ca2+ channels and delayed-rectifier K + channels. Differentiated cells also possessed functional glutamate, GABA, glycine and purinergic (P2X) receptors. Appearance of voltage-dependent and ligand-gated ion channels appears to be an important early step in the differentiation of NPCs.

Published
2004

99. Defining a Threshold Surface Density of Vitronectin for the Stable Expansion of Human Embryonic Stem Cells

Author

Yap, Lynn Y.W., primary, Li, Jian, additional, Phang, In Yee, additional, Ong, Lay Ting, additional, Ow, Jo'an Zhu-En, additional, Goh, James C.H., additional, Nurcombe, Victor, additional, Hobley, Jonathan, additional, Choo, Andre B.H., additional, Oh, Steve K.W., additional, Cool, Simon M., additional, and Birch, William R., additional

Published
2011
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