90 results on '"Chen, Qing-Yu"'
Search Results
52. Columnar Structured YSZ Coating Formation by PS-PVD Using Conventional Plasma Spray System Assisted Through Droplet Filtering
- Author
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Chen, Qing-Yu, additional, Li, Cheng-Xin, additional, Li, Chang-Jiu, additional, Luo, Xiao-Tao, additional, and Yang, Guan-Jun, additional
- Published
- 2015
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53. Microstructure of YSZ Coatings Deposited by PS-PVD Using 45 kW Shrouded Plasma Torch
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Chen, Qing-Yu, primary, Li, Cheng-Xin, additional, Zhao, Jian-Zhuang, additional, Yang, Guan-Jun, additional, and Li, Chang-Jiu, additional
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- 2015
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54. Controlling grain size in columnar YSZ coating formation by droplet filtering assisted PS-PVD processing
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Chen, Qing-Yu, primary, Li, Cheng-Xin, additional, Wei, Tao, additional, Sun, Hai-Bin, additional, Zhang, Shan-Lin, additional, Luo, Xiao-Tao, additional, Yang, Guan-Jun, additional, Li, Chang-Jiu, additional, and Liu, Mei-Lin, additional
- Published
- 2015
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55. Microstructure of YSZ Coatings Deposited by PS-PVD Using 45 kW Shrouded Plasma Torch.
- Author
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Chen, Qing-Yu, Li, Cheng-Xin, Zhao, Jian-Zhuang, Yang, Guan-Jun, and Li, Chang-Jiu
- Subjects
YTTRIA stabilized zirconium oxide ,PLASMA sprayed coatings ,PLASMA spraying ,PLASMA torch ,MICROSTRUCTURE ,PLASMA jets ,EMISSION spectroscopy ,EQUIPMENT & supplies - Abstract
In this study, a conventional 80 kW class plasma spraying system was used to produce yttria-stabilized-zirconia (YSZ) coatings by PS-PVD at a pressure of 100 Pa. A shroud was attached in the front of the plasma nozzle to restrain expansion of plasma jet. The torch was operated at an arc power of 45 kW and YSZ coatings were deposited at a powder feed rate of 0.2 g/min. Optical emission spectroscopy was used to diagnose the particle state in plasma jet. The surface morphology and cross-sectional morphology of coatings was characterized by field emission scanning electron microscope. It is found that the amount of YSZ evaporation is significantly enhanced through using a shroud. The coatings with a hybrid microstructure of splats and nanoclusters were deposited perpendicular to the coatings. The nanostructured clusters deposited out of the vapor are presented at splat interfaces. It is evident that using powders specially designed for PS-PVD and controlling heating of plasma jet to spray particles, PS-PVD deposition for a hybrid microstructure consisting of vapor phase deposit can be realized through conventional plasma spray system. Columnar grain structured YSZ can also be deposited by pure vapor phase at the side surface of the substrate. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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56. Controlling grain size in columnar YSZ coating formation by droplet filtering assisted PS-PVD processing.
- Author
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Chen, Qing-Yu, Li, Cheng-Xin, Wei, Tao, Sun, Hai-Bin, Zhang, Shan-Lin, Luo, Xiao-Tao, Yang, Guan-Jun, Li, Chang-Jiu, and Liu, Mei-Lin
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- 2016
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57. Antitumor and anti-angiogenesis effects of thymoquinone on osteosarcoma through the NF-κB pathway
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PENG, LEI, primary, LIU, AN, additional, SHEN, YUE, additional, XU, HUA-ZI, additional, YANG, SHI-ZHOU, additional, YING, XIAO-ZHOU, additional, LIAO, WEI, additional, LIU, HAI-XIAO, additional, LIN, ZHONG-QIN, additional, CHEN, QING-YU, additional, CHENG, SHAO-WEN, additional, and SHEN, WEI-DONG, additional
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- 2012
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58. Indentation Deformation and Microcracking in β‐Si3N4‐Based Nanoceramic
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Lee, Ching‐Huan, primary, Liu, Hao‐Chih, additional, Lu, Horng‐Hwa, additional, Goto, Takashi, additional, Tu, Rong, additional, Wang, Chang‐An, additional, Pavol, Sajalik, additional, Ruan, Jian‐Long, additional, Nayak, Pramoda K., additional, Chen, Jian‐Horn, additional, Chen, Qing‐Yu, additional, and Huang, Jow‐Lay, additional
- Published
- 2012
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59. Influence of Powder Type and Substrate Hardness on the Deposition Behavior of WC-17Co Particle in Cold Spraying
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Wang, Hong Tao, primary, Ji, Gang Chang, additional, Chen, Xiao, additional, Chen, Qing Yu, additional, Bai, Xiao Bo, additional, Yang, Gui Bin, additional, Yang, Feng Gen, additional, and Deng, Bang Hua, additional
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- 2011
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60. The Microstructure and Erosion Wear Performance of HVOF-Sprayed Cr3C2-NiCr Coatings with Different Feedstock Powder
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Wang, Hong Tao, primary, Ji, Gang Chang, additional, Chen, Qing Yu, additional, Du, Xue Fei, additional, and Fu, Wei, additional
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- 2011
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61. Microstructure Characterization and Abrasive Wear Performance of HVOF Sprayed WC-Co Coatings
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Wang, Hong Tao, primary, Ji, Gang Chang, additional, Chen, Qing Yu, additional, Du, Xue Fei, additional, and Fu, Wei, additional
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- 2011
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62. Microstructure and Abrasive Wear Behaviour of HVOF Sprayed Carbide-Based Cermet Coatings
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Wang, Hong Tao, primary, Ji, Gang Chang, additional, Chen, Qing Yu, additional, Du, Xue Fei, additional, and Fu, Wei, additional
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- 2011
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63. Nitroglycerin in the Prevention of Post-ERCP Pancreatitis: A Meta-Analysis
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Shao, Li-Ming, primary, Chen, Qing-Yu, additional, Chen, Miao-Yan, additional, and Cai, Jian-Ting, additional
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- 2009
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64. Proliferation, differentiation and BMP-2 expression of osteoblasts cultured in the plane under suitable electrical stimulation.
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Wang Wei, Li Xiu-Cui, Cheng Shao-Wen, Chen Qing-Yu, Peng Lei, Lin Zhong-Qin, Zhang Wei, and Kou Dong-Quan
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- 2012
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65. Use of titanium elastic intramedullary nails in the treatment of adult midshaft clavicular fractures: technical skills, precautions and complications.
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Zhang Wei, Chen Qing-yu, Kou Dong-quan, Cheng Shao-wen, Zhao Jing-kai, Qi Ying-jie, and Peng Lei
- Published
- 2012
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66. Indentation Deformation and Microcracking in β- Si3 N4-Based Nanoceramic.
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Lee, Ching-Huan, Liu, Hao-Chih, Lu, Horng-Hwa, Goto, Takashi, Tu, Rong, Wang, Chang-An, Pavol, Sajalik, Ruan, Jian-Long, Nayak, Pramoda K., Chen, Jian-Horn, Chen, Qing-Yu, Huang, Jow-Lay, and Pharr, G.
- Abstract
The effects of microstructure on the mechanical responses and damage evolution of spark-plasma-sintered β- Si
3 N4 -based ceramics has been evaluated through indentation tests. It was found that the nanoceramic and its coarse-grained counterpart exhibit similar elastoplastic behavior in their indentation responses. However, the increased hardness and ratio of elastic work to total work done in the nanoceramic suggest that resistance to plastic deformation is greater than that in the coarser-grained one. The microcracking behavior within the indented materials is also proposed. A finite-element analysis is used to estimate the distribution of compressive and shear stresses within the indented materials. The smaller grain size in bridging ceramic not only enhances the energy dissipation by formation of a higher density of intergranular microcracks along the weak grain boundary phase but also toughens the cracked solid through increasing resistance to plastic sliding and multi-cracks propagation. Prior to formation of the radial cracks, better damage tolerance of nanoceramic is thought to be achieved in this study. [ABSTRACT FROM AUTHOR]- Published
- 2012
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67. A Feminist Interpretation of Jungian Psychology.
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Guo Ai-Mei and Chen Qing-Yu
- Subjects
JUNGIAN psychology ,FEMINISM ,PERSONALITY ,GENDER ,PSYCHOTHERAPY - Abstract
In this paper, we re-examined Jungian analytical psychology from a feminist perspective. When reflecting on and exploring the feminist thoughts embedded in Jungian psychology, we focused on its influence on the development of feminism and post modernism. Jungian androgynous personality theory makes it possible for us to understand the social construction of gender, and his archetypal and goddess psychology has promoted the development of cultural feminism and feminist archetypal psychology. Jung's theory of self dovetails with the postmodern theory of self and his views on psychotherapy are also in line with feminist psychotherapies. [ABSTRACT FROM AUTHOR]
- Published
- 2012
68. Effects of nicotine on bone marrow stromal cells proliferation and differentiation of chondrocyte in vitro.
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Ying Xiaozhou, Peng Lei, Cheng Shao-wen, Chen Qing-yu, Zhang Wei, Kou Dong-quan, and Shen Yue
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- 2011
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69. Intramedullary nailing of clavicular midshaft fractures in adults using titanium elastic nail.
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Qing-yu, Chen, Dong-quan, Kou, Xiao-jie, Cheng, Wei, Zhang, Wei, Wang, Zhang-qin, Lin, Shao-wen, Cheng, Yue, Shen, Xiao-zhou, Ying, Lei, Peng, Chuan-zhu, Lü, Yuan, Tian, Chen, Qing-Yu, Kou, Dong-Quan, Cheng, Xiao-Jie, Zhang, Wei, Wang, Wei, Lin, Zhang-Qin, Cheng, Shao-Wen, and Shen, Yue
- Abstract
Objective: Studies showed elastic stable intramedullary nailing (ESIN) of displaced midclavicular fractures has excellent outcomes, as well as high complication rates and specific problems. The aim was to discuss ESIN of midshaft clavicular fractures.Methods: Totally 60 eligible patients (aged 18-63 years) were randomized to either ESIN group or non-operative group between January 2007 and May 2008. Clavicular shortening was measured after trauma and osseous consolidation. Radiographic union and complications were assessed. Function analysis including Constant shoulder scores and disabilities of the arm, shoulder and hand (DASH) scores were performed after a 15-month follow-up.Results: ESIN led to a signifcantly shorter time to union, especially for simple fractures. In ESIN group, all patients got fracture union, of which 5 cases had medial skin irritation and 1 patient needed revision surgery because of implant failure. In the nonoperative group, there were 3 nonunion cases and 2 symptomatic malunions developed requiring corrective osteotomy. At 15 months after intramedullary stabilization, patients in the ESIN group were more satisfied with the appearance of the shoulder and overall outcome, and they benefited a lot from the great improvement of post-traumatic clavicular shortening. Furthermore, DASH scores were lower and Constant scores were significantly higher in contrast to the non-operative group.Conclusion: ESIN is a safe minimally invasive surgical technique with lower complication rate, faster return to daily activities, excellent cosmetic and better functional results, restoration of clavicular length for treating mid-shaft clavicular fractures, resulting in high overall satisfaction, which can be regard as an alternative to plate fixation or nonoperative treatment of mid-shaft clavicular fractures. [ABSTRACT FROM AUTHOR]- Published
- 2011
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70. Osteogenic capability of autologous rabbit adipose-derived stromal cells in repairing calvarial defects.
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Shao-wen, Cheng, Wei, Wang, Wei, Zhang, Dong-quan, Kou, Xiao-zhou, Ying, Qing-yu, Chen, Yue, Shen, Xiao-jie, Cheng, Zhong-qin, Lin, Lei, Peng, Chuan-zhu, Lü, Cheng, Shao-Wen, Lin, Zhong-Qin, Wang, Wei, Zhang, Wei, Kou, Dong-Quan, Ying, Xiao-Zhou, Chen, Qing-Yu, Shen, Yue, and Cheng, Xiao-Jie
- Abstract
Objective: To evaluate the in vitro and in vivo osteogenic capability of adipose-derived stromal cells (ASCs).Methods: ASCs were isolated from New Zealand white rabbits and determined by alkaline phosphatase (ALP) staining, von Kossa staining and alizarin red staining. Some specific markers of osteogenic differentiation, including ALP, osteocalcin (OCN), osteopontin (OPN) were examined by reverse transcription-polymerase chain reaction (RT-PCR). In vivo, demineralized bone matrix (DBM)-ASCs composites were implanted into the rabbit calvarial defects created at each side of the longitudinal midline. After 6 weeks, histologic properties of the transplants were analyzed.Results: ASCs were successfully induced into osteogenesis. ALP staining, von Kossa staining and alizarin red staining showed positive results. The expressions of ALP, OCN and OPN were detected in ASCs after cultivation in osteogenic medium. Extensive new bone was observed in the defects transplanted with DBM-ASCs composites.Conclusion: ASCs have the potential to differentiate into osteogenic lineage and DBM-ASCs constructs are a promising method for regeneration in bone defects. [ABSTRACT FROM AUTHOR]- Published
- 2011
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71. Nitroglycerin in the prevention of post-ERCP pancreatitis: a meta-analysis.
- Author
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Li-Ming Shao, Qing-Yu Chen, Miao-Yan Chen, Jian-Ting Cai, Shao, Li-Ming, Chen, Qing-Yu, Chen, Miao-Yan, and Cai, Jian-Ting
- Subjects
ENDOSCOPIC retrograde cholangiopancreatography ,THERAPEUTIC use of nitroglycerin ,CONDOMS ,PANCREATITIS ,META-analysis ,RANDOMIZED controlled trials ,DATABASES ,EQUIPMENT & supplies ,PREVENTION ,DUODENUM ,NITROGLYCERIN ,SYSTEMATIC reviews - Abstract
The objective of this research paper is to evaluate the effect of prophylactic nitroglycerin in the prevention of post-endoscopic retrograde cholangiopancreatography (ERCP) pancreatitis (PEP) by performing a meta-analysis of randomized controlled trials (RCTs). Electronic databases, including PubMed, EMBASE, the Cochrane library, and the Science Citation Index, were searched to retrieve relevant trials. Outcome measures were the incidence of PEP. Four RCTs, enrolling a total of 856 patients, were included. Meta-analysis of these trials indicated a significant association between the use of nitroglycerin and the reduction of PEP (RR 0.60; 95%CI: 0.39-0.92; P = 0.02). However, subsequent sensitive analysis failed to confirm that nitroglycerin was statistically superior to a placebo in reducing PEP (RR 0.68; 95%CI: 0.41-1.11; P = 0.12). Based on the limitations in this meta-analysis, prophylactic use of nitroglycerine for all patients who underwent ERCP is not recommended. Further clinical trials are required to confirm the effect of nitroglycerin in the prevention of PEP. [ABSTRACT FROM AUTHOR]
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- 2010
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72. Coccygectomy for stubborn coccydynia
- Author
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CHENG Shao-wen, CHEN Qing-yu, LIN Zhong-qin, WANG Wei, ZHANG Wei, KOU Dong-quan, SHEN Yue, YING Xiao-zhou, CHENG Xiao-jie, LÜ Chuan-zhu, and Peng Lei
- Subjects
lcsh:R5-920 ,Surgical procedures, operative ,Coccyx ,Pain ,lcsh:Medicine (General) - Abstract
【Abstract】Objective: To evaluate the preliminary clinical outcomes of coccygectomy in patients with coccydynia after a failure of conservative treatment. Methods: From May 2002 to January 2010, 31 patients with coccydynia were treated by coccygectomy in our department after conservative measures had failed to produce significant relief. A questionnaire, which included the extent of relief in the painful area, improvement in quality of life, intensity of pain in the sitting position, and pain score during daily activities, was used to evaluate the results. Results: All patients were followed up for 1 to 6 years (mean 3.3 years). The results were excellent in 20 patients (64.5%), good in 7 patients (22.6%), moderate in 3 patients (9.7%) and poor in 1 patient (3.2%). The excellent and good rates amounted to 87.1%. All patients except one had complete resolution of their symptoms and were subjectively highly satisfied with the outcomes of the surgery. Only 2 cases of superficial infection were observed postoperatively. Conclusion: Coccygectomy is a feasible management option for patients with coccygodynia that has no response to conservative treatments. Key words: Coccyx; Pain; Surgical procedures, operative
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73. K-wire and tension band wire fixation in treating sternoclavicular joint dislocation
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CHEN Qing-yu, CHENG Shao-wen, WANG Wei, LIN Zhong-qin, ZHANG Wei, KOU Dong-quan, SHEN Yue, YING Xiao-zhou, CHENG Xiao-jie, LÜ Chuan-zhu, and PENG Lei
- Subjects
Sternoclavicular joint ,lcsh:R5-920 ,Dislocations ,lcsh:Medicine (General) ,Bone wires ,Fracture fixation, internal - Abstract
【Abstract】Objective: To evaluate the feasibility and therapeutic effect of treating sternoclavicular joint dislocation by K-wire and tension band wire fixation, and to improve the safety and stability of this technique. Methods: This study consisted of 9 cases, 6 males and 3 females with the mean age of 25 years (range, 9-62 years). The causes were traffic accident in 7 cases, falling in 1 case and fight in 1 case. The duration from injury to operation was 2 hours to 7 days. There were 5 left dislocations and 4 right dislocations; 8 anterior dislocations and 1 posterior dislocation, including one combined with left scapular fracture and one with left olecranon fracture. Open reduction and internal fixation using K-wires and tension band wires were performed to treat dislocations. Results: All patients were followed up for 6 to 24 months, 10 months on average. According to Rockwood’s rating scale on postoperative sternoclavicular joint, 8 cases achieved excellent outcomes with an average score of 13.88, and the rest case achieved a good outcome with the score of 12. Anatomical reduction was obtained in all cases. There were no such postoperative complications as severe infection, injury to blood vessel and nerve, failure of fixation, etc. Patients were all satisfied with the anatomical reduction and functional recovery. Conclusions: The technique of K-wire and tension band wire fixation is safe, simple, effective, less invasive and has been successfully used in orthopedic surgery. It is effective in treating sternoclavicular joint dislocation though it has some disadvantages. Key words: Sternoclavicular joint; Dislocations; Bone wires; Fracture fixation, internal
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74. Osteogenic capability of autologous rabbit adipose-derived stromal cells in repairing calvarial defects
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CHENG Shao-wen, LIN Zhong-qin, WANG Wei, ZHANG Wei, KOU Dong-quan, YING Xiao-zhou, CHEN Qing-yu, SHEN Yue, CHENG Xiao-jie, PENG Lei, and LV Chuan-zhu
75. Long Noncoding RNA MSL3P1 Regulates CUL3 mRNA Cytoplasmic Transport and Stability and Promotes Lung Adenocarcinoma Metastasis.
- Author
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Shao MM, Li X, Wei RQ, Chen QY, Zhang X, Qiao X, and Li H
- Subjects
- Humans, Animals, Mice, RNA, Messenger genetics, RNA, Messenger metabolism, Male, Cell Line, Tumor, Cytoplasm metabolism, Prognosis, Gene Expression Regulation, Neoplastic, Mice, Nude, Female, Epithelial-Mesenchymal Transition genetics, RNA, Long Noncoding genetics, RNA, Long Noncoding metabolism, Cullin Proteins metabolism, Cullin Proteins genetics, Adenocarcinoma of Lung genetics, Adenocarcinoma of Lung pathology, Adenocarcinoma of Lung metabolism, Lung Neoplasms genetics, Lung Neoplasms pathology, Lung Neoplasms metabolism, Lung Neoplasms secondary, RNA Stability, Neoplasm Metastasis
- Abstract
Lung adenocarcinoma (LUAD) is the most prevalent histological type of lung cancer. Previous studies have reported that specific long noncoding RNAs (lncRNA) are involved in cancer development and progression. The phenotype and mechanism of ENST00000440028, named MSL3P1, an lncRNA referred to as a cancer-testis gene with potential roles in tumorigenesis and progression, have not been reported. MSL3P1 is overexpressed in LUAD tumor tissues, which is significantly associated with clinical characteristics, metastasis, and poor clinical prognosis. MSL3P1 promotes the metastasis of LUAD in vitro and in vivo. The enhancer reprogramming in LUAD tumor tissue is the major driver of the aberrant expression of MSL3P1. Mechanistically, owing to the competitive binding to CUL3 mRNA with ZFC3H1 protein (a protein involved in targeting polyadenylated RNA to exosomes and promoting the degradation of target mRNA), MSL3P1 can prevent the ZFC3H1-mediated RNA degradation of CUL3 mRNA and transport it to the cytoplasm. This activates the downstream epithelial-to-mesenchymal transition signaling pathway and promotes tumor invasion and metastasis. Implications: This study indicates that lncRNA MSL3P1 regulates CUL3 mRNA stability and promotes metastasis and holds potential as a prognostic biomarker and therapeutic target in LUAD., (©2024 American Association for Cancer Research.)
- Published
- 2024
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76. Distributed Finite Time Consensus of Secondorder Multi-agent Systems via Pinning Control (August 2018).
- Author
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Zhou YJ, Jiang GP, Xu FY, and Chen QY
- Abstract
The robust distributed finite time consensus of second-order multi-agent systems via pinning control has been investigated in this paper. A new nonsingular finite time TSM control method is proposed for second-order single system with disturbances. Based on the pinning error function vector, robust distributed finite time consensus of second-order multi-agent systems via pinning control method is given. Simulations results are performed to validate the effectiveness of the theoretical results.
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- 2018
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77. Slow-pull and different conventional suction techniques in endoscopic ultrasound-guided fine-needle aspiration of pancreatic solid lesions using 22-gauge needles.
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Chen JY, Ding QY, Lv Y, Guo W, Zhi FC, Liu SD, and Cheng TM
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- Adult, Aged, Aged, 80 and over, Female, Humans, Male, Middle Aged, Neuroendocrine Tumors diagnosis, Pancreatic Neoplasms diagnosis, Reproducibility of Results, Retrospective Studies, Sensitivity and Specificity, Young Adult, Endoscopic Ultrasound-Guided Fine Needle Aspiration methods, Neuroendocrine Tumors pathology, Pancreatic Neoplasms pathology, Suction instrumentation, Suction methods
- Abstract
Aim: To evaluate the cytological diagnostic capacity and sample quality of the slow-pull technique and compare them with different suction techniques., Methods: From July 2010 to December 2015, 102 patients with pancreatic solid lesions who underwent endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) with 22-gauge needles were retrospectively evaluated. EUS-FNA diagnosis was based on a cytological examination, and final diagnosis was based on a comprehensive standard of cytological diagnosis, surgical pathology and clinical or imaging follow-up. Cytological specimens were characterized for cellularity and blood contamination. The cytological diagnostic capacity and sample quality of the slow-pull technique and suction techniques with 5-mL/10-mL/20-mL syringes were analyzed., Results: Of all of the EUS-FNA procedures, the slow-pull technique and suction techniques with 5-mL/10-mL/20-mL syringes were used in 31, 19, 34 and 18 procedures, respectively. There were significant differences between these four suction techniques in terms of cytological diagnostic accuracy (90.3% vs 63.2% vs 58.8% vs 55.6%, P = 0.019), sensitivity (88.2% vs 41.7% vs 40.0% vs 36.4%, P = 0.009) and blood contamination (score ≥ 2 for 29.0% vs 52.6% vs 70.6% vs 72.2%, P = 0.003). The accuracy and sensitivity of the slow-pull technique were significantly higher than those of the suction techniques using 5-mL ( P = 0.03, P = 0.014), 10-mL ( P = 0.005; P = 0.006) and 20-mL syringes ( P = 0.01, P = 0.01). Blood contamination was significantly lower in the slow-pull technique than in the suction techniques with 10-mL ( P = 0.001) and 20-mL syringes ( P = 0.007)., Conclusion: The slow-pull technique may increase the cytological diagnostic accuracy and sensitivity with slight blood contamination during EUS-FNA when using 22-gauge needles for solid pancreatic masses., Competing Interests: Conflict-of-interest statement: Each author certifies that he or she has no commercial associations that might pose a conflict of interest in connection with the submitted article.
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- 2016
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78. Gastric foregut cystic developmental malformation: case series and literature review.
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Geng YH, Wang CX, Li JT, Chen QY, Li XZ, and Pan H
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- Biopsy, Cysts classification, Cysts diagnosis, Cysts therapy, Gastrectomy, Humans, Lymph Node Excision, Male, Middle Aged, Predictive Value of Tests, Stomach Diseases classification, Stomach Diseases diagnosis, Stomach Diseases surgery, Terminology as Topic, Tomography, X-Ray Computed, Treatment Outcome, Cysts congenital, Gastric Mucosa abnormalities, Stomach Diseases congenital
- Abstract
Foregut cystic developmental malformation (FCDM) is a very rare lesion of the alimentary tract, especially in the stomach. We discuss the concepts of gastric duplication cyst, bronchogenic cysts, and FCDM. Nomenclature has been inconsistent and confusing, but, by some definitions, gastric duplication cysts involve gastric mucosa and submucosal glands, bronchogenic cysts involve respiratory mucosa with underlying cartilage and glands, and FCDM lacks gastric mucosa or underlying glands or cartilage but has pseudostratified ciliated columnar epithelium (PCCE). We searched our departmental case files from the past 15 years and identified 12 cases of FCDM in the alimentary tract. We summarize the features of these 12 cases including a report in detail on a 52-year-old man with a submucosal cyst lined with simple PCCE and irregular and stratified circular muscle layers that merged with gastric smooth muscle bundles near the lesser curvature of the gastric cardia. A literature review of cases with this histology yielded 25 cases. We propose the term gastric-FCDM for such cases. Our own series of 12 cases confirms that preoperative recognition of the entity is infrequent and problematic. The rarity of this developmental disorder, as well as a lack of understanding of its embryologic origins, may contribute to missing the diagnosis. Not appreciating the diagnosis preoperatively can lead to an inappropriate surgical approach. In contrast, presurgical recognition of the entity will contribute to a good outcome and reduced risk of complications.
- Published
- 2015
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79. Antitumor and anti-angiogenesis effects of thymoquinone on osteosarcoma through the NF-κB pathway.
- Author
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Peng L, Liu A, Shen Y, Xu HZ, Yang SZ, Ying XZ, Liao W, Liu HX, Lin ZQ, Chen QY, Cheng SW, and Shen WD
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- Animals, Antigens, CD34 metabolism, Antineoplastic Agents pharmacology, Apoptosis drug effects, Benzoquinones pharmacology, Bone Neoplasms pathology, Caspase 3 metabolism, Cell Line, Tumor, Cell Proliferation drug effects, Cell Survival drug effects, Human Umbilical Vein Endothelial Cells drug effects, Humans, Inhibitor of Apoptosis Proteins metabolism, Ki-67 Antigen metabolism, Male, Mice, Mice, Inbred BALB C, Neovascularization, Pathologic drug therapy, Oligopeptides metabolism, Osteosarcoma pathology, Signal Transduction drug effects, Survivin, X-Linked Inhibitor of Apoptosis Protein metabolism, Antineoplastic Agents therapeutic use, Benzoquinones therapeutic use, Bone Neoplasms blood supply, Bone Neoplasms drug therapy, NF-kappa B metabolism, Osteosarcoma blood supply, Osteosarcoma drug therapy
- Abstract
Thymoquinone (TQ), the predominant bioactive constituent derived from the medicinal spice Nigella sativa (also known as black cumin), has been applied for medical purposes for more than 2,000 years. Recent studies reported that thymoquinone exhibited inhibitory effects on the cell proliferation of several cancer cell lines. This study was performed to investigate the antitumor and anti-angiogenic effects of thymoquinone on osteosarcoma in vitro and in vivo. Our results showed that thymoquinone induced a higher percentage of growth inhibition and apoptosis in the human osteosarcoma cell line SaOS-2 compared to that of control, and thymoquinone significantly blocked human umbilical vein endothelial cell (HUVEC) tube formation in a dose-dependent manner. To investigate the possible mechanisms involved in these events, we performed electrophoretic mobility shift assay (EMSA) and western blot analysis, and found that thymoquinone significantly downregulated NF-κB DNA-binding activity, XIAP, survivin and VEGF in SaOS-2 cells. Moreover, the expression of cleaved caspase-3 and Smac were upregulated in SaOS-2 cells after treatment with thymoquinone. In addition to these in vitro results, we also found that thymoquinone inhibits tumor angiogenesis and tumor growth through suppressing NF-κB and its regulated molecules. Collectively, our results demonstrate that thymoquinone effectively inhibits tumor growth and angiogenesis both in vitro and in vivo. Moreover, inhibition of NF-κB and downstream effector molecules is a possible underlying mechanism of the antitumor and anti-angiogenic activity of thymoquinone in osteosarcoma.
- Published
- 2013
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80. [Proliferation, differentiation and BMP-2 expression of osteoblasts cultured in the plane under suitable electrical stimulation].
- Author
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Wang W, Li XC, Cheng SW, Chen QY, Peng L, Lin ZQ, Zhang W, and Kou DQ
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- Animals, Cell Proliferation, Rabbits, Bone Morphogenetic Protein 2 metabolism, Cell Culture Techniques methods, Cell Differentiation, Electric Stimulation, Gene Expression Regulation, Osteoblasts cytology, Osteoblasts metabolism
- Abstract
Objective: To observe proliferation and differentiation of osteoblasts cultured in the plane on appropriate electrical stimulation, to specify whether it promote the proliferation, and observe expression of BMP-2 on electrical stimulation., Methods: Osteoblasts were extracted from the skull of rabbit offspring and cultured. Cells after the 2nd generations were cultured. In experimental group, cells had electrical stimulation, and same stimulation time and intensity were given. In control group cells had not electrical stimulation. The proliferation and differentiation were detected at different time, and BMP-2 protein expression was analyzed., Results: The cell morphology of experimental group in 8 days under the light microscope was observed and showed a lot of proliferation of osteoblasts, pleomorphic changes, in 6 to 8 days a small amount of Calcified spots was also observed; while in the control group, proliferation was slower. Differentiation of the experimental group was significantly, alkaline phosphatase staining and calcium nodules were positive, quantitative analysis of alkaline phosphatase increaseed significantly. Experimental group showed that BMP-2 was gradually increased by immunohistochemistry analysis., Conclusion: Electrical stimulation can promote the proliferation and differentiation of osteoblasts and achieved the increasement the number of cells in short-term, intracellular staining by immunohistochemistry showed the increasement in expression of BMP-2.
- Published
- 2012
81. [Use of titanium elastic intramedullary nails in the treatment of adult midshaft clavicular fractures: technical skills, precautions and complications].
- Author
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Zhang W, Chen QY, Kou DQ, Cheng SW, Zhao JK, Qi YJ, and Peng L
- Subjects
- Adult, Aged, Clavicle surgery, Female, Humans, Male, Middle Aged, Clavicle injuries, Fracture Fixation, Intramedullary methods, Fractures, Bone surgery
- Abstract
Objective: To discuss surgical skills,precautions and complications of using titanium elastic intramedullary nails in the treatment of adult midshaft clavicular fractures and evaluate the therapeutic efficacy., Methods: From June 2006 to January 2009, 28 patients with completely displaced midshaft clavicular fractures (15 simple fractures, 8 wedge fractures and 5 comminuted fractures) were enrolled in the study,included 19 males and 9 females with a mean age of 39.0 years (range 19-67 years). The injury was on the left side in 14 cases and on the right side in 14 cases. The mean course of disease was 2.9 days. The shoulders of the patients were swollen, deformed and disabled before operation. X-rays revealed complete displacement of the clavicle. The mean clavicular shortening after injury was 6.76% vs. that measured after bone healing. The Constant-Murley Shoulder Scoring System was used to assess shoulder function, and the DASH Score was used to assess the disability degree of the upper arm., Results: Closed operation with titanium elastic intramedullary nails was undertaken in 26 cases, and open reduction was performed in the remaining two cases. Satisfactory reduction was achieved in all patients, who were followed up for a mean of 10 months (range 6-15 months). The mean union time was 11.5 weeks. No severe complication occurred in any patient. The mean clavicular shortening after bone healing was 3.38%, which was significantly different as compared with the mean clavicular shortening after injury. Constant-Murley Shoulder Score was (97.0 +/- 4.2), and DASH score was (3.4 +/- 4.8). Anatomical reduction, functional recovery and appearance were satisfactory in all patients., Conclusion: Treatment of adult midshaft clavicular fractures with titanium elastic intramedullary nails is advantageous and may prove to be an alternative option for nonsurgical treatment and plate osteosynthesis of midshaft clavicular fractures in adults.
- Published
- 2012
82. [Effect of metoclopramide on capsule endoscopy examination: a randomized study].
- Author
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Chen JM, Zhong DD, Xie CG, Chen QY, Chen Y, Du Q, and Cai JT
- Subjects
- Adult, Female, Gastrointestinal Transit drug effects, Humans, Male, Middle Aged, Capsule Endoscopy, Metoclopramide therapeutic use
- Abstract
Objective: To investigate the effect of metoclopramide on capsule endoscopy (CE) examination., Methods: Total 116 patients referred for CE were randomized into two groups with 58 patients in each group. In treatment group patients received 10 mg metoclopramide intramuscular injection after swallowing the capsule and in control group no metoclopramide was administered. The gastric transit time, small bowel transit time, complete endoscopy rate were observed in both groups., Results: The CE examination was completed in 51 patients of treatment group (87.9%) and 48 of control group (84.2%). Mean gastric transit time was (32.45 ± 29.63) min in treatment group and (45.81 ± 40.01)min in control group, there was significant difference between two groups (P<0.05). Mean small bowel transit time was (252.69 ± 113.29) min in treatment group and (258.75 ± 83.83) min in control group, there was no significant difference between two groups (P>0.05)., Conclusion: Metoclopramide may reduces gastric transit time, but not effect small bowel transit time,which suggests that it might increase the likelihood of complete small-bowel examination in patients undergoing capsule endoscopy.
- Published
- 2012
- Full Text
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83. [Effects of nicotine on bone marrow stromal cells proliferation and differentiation of chondrocyte in vitro].
- Author
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Ying XZ, Peng L, Cheng SW, Chen QY, Zhang W, Kou DQ, and Shen Y
- Subjects
- Aggrecans genetics, Animals, Bone Marrow Cells physiology, Cell Differentiation drug effects, Cell Proliferation drug effects, Chondrocytes cytology, Collagen Type II genetics, Male, RNA, Messenger analysis, Rabbits, Stromal Cells drug effects, Stromal Cells physiology, Bone Marrow Cells drug effects, Chondrocytes drug effects, Nicotine pharmacology
- Abstract
Objective: To examine the effects of various concentration of nicotine on bone marrow stromal cells (BMSCs) proliferation and differentiation of cartilaginous in vitro., Methods: BMSCs was obtained from femoral bone and tibia of New-Zealand albino rabbit. The cells of the 3rd generation were used in study. Different concentration of nicotine (0, 1 x 10(-7), 1 x 10(-6), 1 x 10(-5) M) were added into BMSCs. BMSCs proliferation was analyzed by MTT assay at the 1, 4, 7, 14 days. The expression of collagen type II and aggrecan as the marker genes of cartilaginous differentiation from BMSCs were detected by reverse transcriptase-polymerase chain reaction (RT-PCR)., Results: Microscope showed that BMSCs transformed from round to fusiform shape. The concentration of nicotine in 1 x 10(-7), 1 x 10(-6) M had a significant positive effect on cell proliferation and the expression of type II collagen in a time-dependent manner when supplemented in commonly used induction media (P<0.05). Concentrations of nicotine in 1 x 10(-7) can promote the expression of aggrecan at the 7th day after induction,and in 1 x 10(-5) M may inhibit the expression of type II collagen and aggrecan., Conclusion: It was implied that local application of nicotine at an appropriate concentration may be a promising approach for enhancing cartilaginous differentiation capacity of BMSCs in cartilage tissue engineering.
- Published
- 2011
84. [Nine cases of hookworm infection in the small intestine detected by capsule endoscopy].
- Author
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Chen JM, Chen QY, and Chen Y
- Subjects
- Adult, Aged, Ancylostomiasis parasitology, Female, Humans, Male, Middle Aged, Ancylostomiasis diagnosis, Capsule Endoscopy, Intestine, Small parasitology
- Published
- 2011
85. Intramedullary nailing of clavicular midshaft fractures in adults using titanium elastic nail.
- Author
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Chen QY, Kou DQ, Cheng XJ, Zhang W, Wang W, Lin ZQ, Cheng SW, Shen Y, Ying XZ, Peng L, and Lv CZ
- Subjects
- Adult, Clavicle, Humans, Nails, Treatment Outcome, Fracture Fixation, Intramedullary, Titanium
- Abstract
Objective: Studies showed elastic stable intramedullary nailing (ESIN) of displaced midclavicular fractures has excellent outcomes, as well as high complication rates and specific problems. The aim was to discuss ESIN of midshaft clavicular fractures., Methods: Totally 60 eligible patients (aged 18-63 years) were randomized to either ESIN group or non-operative group between January 2007 and May 2008. Clavicular shortening was measured after trauma and osseous consolidation. Radiographic union and complications were assessed. Function analysis including Constant shoulder scores and disabilities of the arm, shoulder and hand (DASH) scores were performed after a 15-month follow-up., Results: ESIN led to a signifcantly shorter time to union, especially for simple fractures. In ESIN group, all patients got fracture union, of which 5 cases had medial skin irritation and 1 patient needed revision surgery because of implant failure. In the nonoperative group, there were 3 nonunion cases and 2 symptomatic malunions developed requiring corrective osteotomy. At 15 months after intramedullary stabilization, patients in the ESIN group were more satisfied with the appearance of the shoulder and overall outcome, and they benefited a lot from the great improvement of post-traumatic clavicular shortening. Furthermore, DASH scores were lower and Constant scores were significantly higher in contrast to the non-operative group., Conclusion: ESIN is a safe minimally invasive surgical technique with lower complication rate, faster return to daily activities, excellent cosmetic and better functional results, restoration of clavicular length for treating mid-shaft clavicular fractures, resulting in high overall satisfaction, which can be regard as an alternative to plate fixation or nonoperative treatment of mid-shaft clavicular fractures.
- Published
- 2011
86. Osteogenic capability of autologous rabbit adipose-derived stromal cells in repairing calvarial defects.
- Author
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Cheng SW, Lin ZQ, Wang W, Zhang W, Kou DQ, Ying XZ, Chen QY, Shen Y, Cheng XJ, Peng L, and Lv CZ
- Subjects
- Animals, Cell Differentiation, Cells, Cultured, Rabbits, Stromal Cells, Adipose Tissue, Osteogenesis
- Abstract
Objective: To evaluate the in vitro and in vivo osteogenic capability of adipose-derived stromal cells (ASCs)., Methods: ASCs were isolated from New Zealand white rabbits and determined by alkaline phosphatase (ALP) staining, von Kossa staining and alizarin red staining. Some specific markers of osteogenic differentiation, including ALP, osteocalcin (OCN), osteopontin (OPN) were examined by reverse transcription-polymerase chain reaction (RT-PCR). In vivo, demineralized bone matrix (DBM)-ASCs composites were implanted into the rabbit calvarial defects created at each side of the longitudinal midline. After 6 weeks, histologic properties of the transplants were analyzed., Results: ASCs were successfully induced into osteogenesis. ALP staining, von Kossa staining and alizarin red staining showed positive results. The expressions of ALP, OCN and OPN were detected in ASCs after cultivation in osteogenic medium. Extensive new bone was observed in the defects transplanted with DBM-ASCs composites., Conclusion: ASCs have the potential to differentiate into osteogenic lineage and DBM-ASCs constructs are a promising method for regeneration in bone defects.
- Published
- 2011
87. [Studies on the spectral characteristics of efficient HIV-1 fusion inhibitor C22].
- Author
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Shi J, He JJ, Wang SL, Sun XY, and Chen QY
- Subjects
- Amino Acid Sequence, DNA, Bacterial, Electrophoresis, Polyacrylamide Gel, Escherichia coli, HIV-1 chemistry, Humans, Peptide Fragments analysis, Plasmids, Polymerase Chain Reaction, Protein Engineering methods, HIV Envelope Protein gp41 chemistry, HIV Fusion Inhibitors chemistry, HIV-1 genetics, Mass Spectrometry, Peptide Fragments chemistry
- Abstract
Acquired immune deficiency syndrome (AIDS) is increasing its negative influences on human society and economy. In the present paper, HIV-1 cell fusion peptide inhibitor C22 was expressed and purified based on the C-terminal sequence of HIV-1 membrane fusion glycoprotein gp41. The gene coding for C22 was totally synthesized using gp41 gene as a template and amplified by PCR The cloned C22 gene was confirmed by restriction endonuclease and sequence analysis and then cloned into plasmid pTMHa30-51. The prepared plasmid was transformed into E. coli BL21 (DE3) and the expressing products were analyzed on SDS-PAGE and tested with mass spectrum. The results indicated that C22 showed high HIV-1 fusion inhibiting capacity, meanwhile, with good thermal stability and water-solubility, and showed no cell toxicity in tested concentrations. The spectral characteristics were tested with circular dichroism (CD). When treated at different temperature in solution condition, the content of a helix of C22 increased at 37 degrees C while decreased sharply at 80 degrees C. The peak value changed significantly with different pH values. The content of a helix of C22 decreased as pH varied toward acid and alkali and the random coiling increased, which led to a relaxed structure. This result indicated that the C22 structure is stable with pH 6. This research may provide a theoretic foundation for the new type HIV-1 peptide inhibitor designing and spectral characteristics study.
- Published
- 2008
88. [Anti-oxidative responses of Agrocybe aegerita mycelia to cadmium stress].
- Author
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Wang SH, Zhang H, Fu MQ, Chen QY, Zhou ZY, and Lu XM
- Subjects
- Agaricales enzymology, Agaricales metabolism, Catalase metabolism, Glutathione metabolism, Mycelium enzymology, Mycelium metabolism, Peroxidase metabolism, Superoxide Dismutase metabolism, Agaricales drug effects, Cadmium toxicity, Mycelium drug effects
- Abstract
This paper studied the effects of different concentration Cd on the anti-oxidative enzyme activities and glutathione content in Agrocybe aegerita cultivated in liquid medium. The results indicated that at low concentrations of Cd, the test enzyme activities increased with increasing Cd concentration, being the maximum at 0.1 mmol x L(-1) for CAT, at 0.2 mmol x L(-1) for POD, GR and LOX, and at 0.4 mmol x L(-1) for SOD. At 1.6 mmol x L(-1) of Cd, the activities of POD, CAT and SOD were inhibited markedly. 0.4-1.6 mmol x L(-1) of Cd resulted in an increase of glutathione content, but glutathione disulfide content was less affected. The ascorbate acid content and APX activity were too low to be detectable. The PAGE analysis revealed that 0.1-0.8 mmol x L(-1) of Cd induced the additional isozyme bands of POD, EST and LOX, and increased the intensity of the constitutive isozymes of CAT and SOD. 1.6 mmol x L(-1) of Cd decreased the intensity of the isozymes of POD, CAT and SOD significantly.
- Published
- 2007
89. [Comparison of clinical features of severe acute respiratory syndrome among different transmission generations].
- Author
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Wu W, Wang JF, Jiang SP, Liu PM, Chen QY, Chen WX, Yin SM, Yan L, Zhan J, Chen XL, and Li JG
- Subjects
- Adolescent, Adult, Female, Humans, Male, Middle Aged, Retrospective Studies, Severe Acute Respiratory Syndrome diagnosis, Severe Acute Respiratory Syndrome transmission
- Abstract
Objective: To investigate the differences among various transmission generation of severe acute respiratory syndrome (SARS) by comparing the corresponding clinical data., Methods: The clinical data of 84 patients with SARS were retrospectively studied, 66 women and 18 men, mean age (29.2 +/- 10.3) years old, 96.4% of whom were health care workers. All subjects had exposure to a source patient and the epidemic progressively propagated in a short time. For the infectious chain, we defined these patients who had exposure to a source patient as the primary cases, which included 35 patients (41.7%). Patients who got the disease by exposure to the primary cases were defined as secondary cases, which included 34 patients (40.5%). Similarly, the tertiary cases included 15 patients (17.9%)., Results: (1) No statistical differences in age, sex ratio, incubation period and hospitalization duration among various infectious generations were found (P > 0.05). (2) With descending in infectious generations, the initial temperature lowered, and cases with cough decreased (P < 0.05) with no statistical differences in the peak temperature, other accompanying symptoms and leukopenia (P > 0.05). (3) With descending in infectious generations, the course from the appearance of pulmonary lesions to their resolution shortened (P < 0.05). No differences were found in the maximal involved pulmonary fields, duration from initial fever to appearance of pulmonary lesions and course from the initial pulmonary lesions to their peak among the above three generations (P > 0.05). (4) No statistical differences were found in ways of oxygen therapy and classes of antibiotics prescribed (P > 0.05). With descending in infectious generation, cases treated with methylprednisolone, human gamma-immunoglobulin, interferon-alpha, and antiviral drugs (oral ribavirin or oseltamivir) increased (P < 0.05) and the duration of their use also increased (P < 0.05)., Conclusions: With descending in infectious generations, the clinical features of SARS may become ameliorated.
- Published
- 2004
90. Comparison of clinical course of patients with severe acute respiratory syndrome among the multiple generations of nosocomial transmission.
- Author
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Wu W, Wang JF, Liu PM, Jiang SP, Chen QY, Chen WX, Yin SM, Yan L, Zhan J, Chen XL, and Li JG
- Subjects
- Adult, Contact Tracing, Female, Humans, Male, Personnel, Hospital, Retrospective Studies, Cross Infection physiopathology, Severe Acute Respiratory Syndrome physiopathology, Severe Acute Respiratory Syndrome transmission
- Abstract
Background: Severe acute respiratory syndrome (SARS) is characterized by both an atypical pneumonia and efficient nosocomial transmission. However, it remains unknown whether the infectivity and the virulence of the pathogen will change throughout the successive transmission. This study was conducted to compare the clinical features and management regimens of patients with SARS among the multiple generations from nosocomial transmission initiated by a super-spreader., Methods: The clinical data of 84 epidemiologically-linked SARS patients from a hospital outbreak were retrospectively studied. All patients, in whom a clear-cut transmission generation could be noted, had a direct or indirect exposure to the index patient and the epidemic successively propagated through the multiple generations of cases within a short period of time., Results: There were 66 women and 18 men with mean age of (29.2 +/- 10.3) years in this cluster; and 96.4% of whom were health care workers. Detailed contact tracing identified 35 (41.7%) first-generation cases, 34 (40.5%) second-generation cases, and 15 (17.8%) third-generation cases. No statistical differences among the multiple generations of transmission were found in terms of age, gender, incubation period and length of hospital stay. With the advanced transmission generations, the initial temperature lowered, the number of cases with dry cough decreased. There were no statistical differences in the peak temperature and duration of fever, other accompanying symptoms, leucopenia; however, the time from initial pulmonary infiltrates to radiographic recovery shortened (P < 0.05). No differences were found in maximum number of lung fields involved, duration from the onset of fever to the occurrence of pulmonary infiltrates and time from the initial pulmonary infiltrate to its peak among the multiple transmission generations (P > 0.05). No statistical differences were found in modes of oxygen therapy and sorts of antibiotics prescribed among the various transmission generations (P > 0.05); however, as with the advanced transmission generations, the number of cases prescribed with methylprednisolone, human gamma-globulin, interferon-alpha, antiviral drugs (oral ribavirin or oseltamivir) increased (P < 0.05) and time from admission to starting these medication shortened (P < 0.05)., Conclusions: There is no evidence that SARS infection will evolve or transmit within a fashion that permits it to become less powerful throughout the successive transmission within a short time.
- Published
- 2004
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