Back to Search
Start Over
Antitumor and anti-angiogenesis effects of thymoquinone on osteosarcoma through the NF-κB pathway.
- Source :
-
Oncology reports [Oncol Rep] 2013 Feb; Vol. 29 (2), pp. 571-8. Date of Electronic Publication: 2012 Dec 04. - Publication Year :
- 2013
-
Abstract
- Thymoquinone (TQ), the predominant bioactive constituent derived from the medicinal spice Nigella sativa (also known as black cumin), has been applied for medical purposes for more than 2,000 years. Recent studies reported that thymoquinone exhibited inhibitory effects on the cell proliferation of several cancer cell lines. This study was performed to investigate the antitumor and anti-angiogenic effects of thymoquinone on osteosarcoma in vitro and in vivo. Our results showed that thymoquinone induced a higher percentage of growth inhibition and apoptosis in the human osteosarcoma cell line SaOS-2 compared to that of control, and thymoquinone significantly blocked human umbilical vein endothelial cell (HUVEC) tube formation in a dose-dependent manner. To investigate the possible mechanisms involved in these events, we performed electrophoretic mobility shift assay (EMSA) and western blot analysis, and found that thymoquinone significantly downregulated NF-κB DNA-binding activity, XIAP, survivin and VEGF in SaOS-2 cells. Moreover, the expression of cleaved caspase-3 and Smac were upregulated in SaOS-2 cells after treatment with thymoquinone. In addition to these in vitro results, we also found that thymoquinone inhibits tumor angiogenesis and tumor growth through suppressing NF-κB and its regulated molecules. Collectively, our results demonstrate that thymoquinone effectively inhibits tumor growth and angiogenesis both in vitro and in vivo. Moreover, inhibition of NF-κB and downstream effector molecules is a possible underlying mechanism of the antitumor and anti-angiogenic activity of thymoquinone in osteosarcoma.
- Subjects :
- Animals
Antigens, CD34 metabolism
Antineoplastic Agents pharmacology
Apoptosis drug effects
Benzoquinones pharmacology
Bone Neoplasms pathology
Caspase 3 metabolism
Cell Line, Tumor
Cell Proliferation drug effects
Cell Survival drug effects
Human Umbilical Vein Endothelial Cells drug effects
Humans
Inhibitor of Apoptosis Proteins metabolism
Ki-67 Antigen metabolism
Male
Mice
Mice, Inbred BALB C
Neovascularization, Pathologic drug therapy
Oligopeptides metabolism
Osteosarcoma pathology
Signal Transduction drug effects
Survivin
X-Linked Inhibitor of Apoptosis Protein metabolism
Antineoplastic Agents therapeutic use
Benzoquinones therapeutic use
Bone Neoplasms blood supply
Bone Neoplasms drug therapy
NF-kappa B metabolism
Osteosarcoma blood supply
Osteosarcoma drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1791-2431
- Volume :
- 29
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Oncology reports
- Publication Type :
- Academic Journal
- Accession number :
- 23232982
- Full Text :
- https://doi.org/10.3892/or.2012.2165