183 results on '"Cazier F"'
Search Results
52. Ground vibration test of F-16 airplane with initial decoupler pylon
- Author
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Cazier, F. W., Jr and Kehoe, M. W
- Subjects
Aircraft Design, Testing And Performance - Abstract
A ground vibration test was conducted on an F-16 airplane loaded on each wing with a 370-gal tank mounted on a standard pylon, a GBU-8 store mounted on a decoupler pylon, and an AIM-9J missile mounted on a wing-tip launcher. The decoupler pylon is a passive wing/store flutter-suppression device. The test was conducted prior to initial flight tests to determine the modal frequencies, mode shapes, and structural damping coefficients. The data presented include frequency response plots, force effect plots, and limited mode shape data.
- Published
- 1984
53. Flutter and steady/unsteady aerodynamic characteristics of supercritical and conventional transport wings
- Author
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Grosser, W. F, Britt, R. T, Childs, C. B, Crooks, O. J, and Cazier, F. W., Jr
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Aircraft Stability And Control - Abstract
This paper presents the technical details and results of a high-speed wind-tunnel test program of an aeroelastic cantilevered transport type wing with two pylon-mounted engines. The tests were conducted in the NASA-Langley 16-foot Transonic Dynamic tunnel (TDT) during December 1981. Flutter of identical planforms, mass properties, and stiffness. The test parameters included different values of model stiffness and wing loading at various angles of attack. The models were instrumented at span-wise wing stations to determine bending and torsion deflections and vertical accelerations. At two model wing stations, pressure transducers were distributed along the chord to record static and unsteady oscillatory pressures during the approach to and onset of flutter. This paper presents the test program with results of the flutter characteristics and selected steady and unsteady aerodynamic data for both airfoils at different angles of attack for various Mach numbers and dynamic pressures.
- Published
- 1983
54. Flutter clearance of the horizontal tail of the Bellanca Skyrocket II airplane
- Author
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Ricketts, R. H, Cazier, F. W., Jr, and Farmer, M. G
- Subjects
Aircraft Design, Testing And Performance - Abstract
The Skyrocket II is an all composite constructed experimental prototype airplane. A flutter clearance program was conducted on the horizontal tail so that the airplane could be safely flown to acquire natural laminar flow aerodynamic data. Ground vibration test data were used in a lifting surface flutter analysis to predict symmetric and antisymmetric flutter boundaries. Subcritical response data which were acquired during flight tests are compared with the analytical results. The final flutter clearance placard speed was based on flight test data.
- Published
- 1982
55. Static and unsteady pressure measurements on a 50 degree clipped delta wing at M = 0.9
- Author
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Hess, R. W, Wynne, E. C, and Cazier, F. W
- Subjects
Aerodynamics - Abstract
Pressures were measured with Freon as the test medium. Data taken at M = 0.9 is presented for static and oscillatory deflections of the trailing edge control surface and for the wing in pitch. Comparisons of the static measured data are made with results computed using the Bailey-Ballhaus small disturbance code.
- Published
- 1982
56. Measured transonic unsteady pressures on an energy efficient transport wing with oscillating control surfaces
- Author
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Cazier, F. W., Jr, Watson, J. J, Doggett, R. V., Jr, Sandford, M. C, and Ricketts, R. H
- Subjects
Aerodynamics - Abstract
Highlight results are presented from subsonic and transonic pressure measurement studies conducted in the Langley Transonic Dynamics Tunnel on a supercritical wing model representative of an energy efficient transport design. Steady- and unsteady-pressure data were acquired on the upper and lower wing surface at an off-design Mach number of 0.60 and at the design Mach number of 0.78, for a Reynolds number of 2.2 x 10(6) (based on the wing average chord). The model configuration consisted of a sidewall-Mounted half-body fuselage and a semi-span wing with an aspect ratio of 10.76, a leading-edge sweepback angle of 28.8 degrees, and supercritical airfoil sections. The wing is instrumented with 252 static pressure orifices and 164 dynamic pressure gages. Model test variables included wing angle of attack, control-surface mean deflection angle, control-surface oscillating deflection angle and frequency, and phasing between oscillating leading-edge and trailing-edge controls when used together.
- Published
- 1981
57. Transonic steady- and unsteady-pressure measurements on a high-aspect-ratio supercritical-wing model with oscillating control surfaces
- Author
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Sandford, M. C, Ricketts, R. H, and Cazier, F. W., Jr
- Subjects
Aerodynamics - Abstract
A supercritical wing with an aspect ratio of 10.76 and with two trailing-edge oscillating control surfaces is described. The semispan wing is instrumented with 252 static orifices and 164 in situ dynamic-pressure gages for studying the effects of control-surface position and motion on steady- and unsteady-pressures at transonic speeds. Results from initial tests conducted in the Langley Transonic Dynamics Tunnel at two Reynolds numbers are presented in tabular form.
- Published
- 1980
58. Passive control of wing/store flutter
- Author
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Reed, W. H., III, Cazier, F. W., Jr, and Foughner, J. T., Jr
- Subjects
Aerodynamics - Abstract
Results are presented for a passive flutter suppression approach known as the decoupler pylon. The decoupler pylon dynamically isolates the wing from store pitch inertia effects by means of soft spring/damper elements assisted by a low frequency feedback control system which minimizes static pitch deflections of the store because of maneuvers and changing flight conditions. Wind tunnel tests and analyses show that this relatively simple pylon suspension system provides substantial increases in flutter speed and reduces the sensitivity of flutter to changes in store inertia and center of gravity. Flutter characteristics of F-16 and YF-17 flutter models equipped with decoupler pylon mounted stores are presented and compared with results obtained on the same model configuration with active flutter suppression systems. These studies show both passive and active concepts to be effective in suppressing wing/store flutter. Also presented are data showing the influence of pylon stiffness nonlinearities on wing/store flutter.
- Published
- 1980
59. Transonic unsteady airloads on an energy efficient transport wing with oscillating control surfaces
- Author
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Sandford, M. C, Ricketts, R. H, Cazier, F. W., Jr, and Cunningham, H. J
- Subjects
Aerodynamics - Abstract
An aspect ratio 10.8 supercritical wing with oscillating control surfaces is described. The wing is instrumental with 252 static orifices and 164 in situ dynamic pressure transducers for studying the effects of control surface deflection on steady and unsteady pressures at transonic speeds. Results from initial wind tunnel tests conducted in the Langley Transonic Dynamics Tunnel are discussed. Unsteady pressure results are presented for two trailing edge control surfaces oscillating separately at the design Mach number of 0.78. Some experimental results are compared with analytical results obtained by using linear lifting surface theory.
- Published
- 1980
60. Behavior of Trifolium repens and Lolium perenne growing in a heavy metal contaminated field: Plant metal concentration and phytotoxicity
- Author
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Bidar, G., primary, Garçon, G., additional, Pruvot, C., additional, Dewaele, D., additional, Cazier, F., additional, Douay, F., additional, and Shirali, P., additional
- Published
- 2007
- Full Text
- View/download PDF
61. Results of ground vibration tests on a YF-12 airplane
- Author
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Wilson, R. J, Cazier, F. W., Jr, and Larson, R. R
- Subjects
Structural Mechanics - Abstract
Ground vibration tests were conducted on a YF-12 airplane. To approximate a structural free-free boundary condition during the tests, each of the landing gears was supported on a support system designed to have a low natural frequency. The test equipment and the procedures used for the ground vibration tests are described. The results are presented in the form of frequency response data, measured mode lines, and elastic mode shapes for the wing/body, rudder, and fuselage ventral fin. In the frequency range between 3.4 cps and 28.8 cps, nine symmetrical wing/body modes, six antisymmetrical wing/body modes, two rudder modes, and one ventral fin mode were measured.
- Published
- 1973
62. Multiple component analysis of cigarette smoke using THz spectroscopy, comparison with standard chemical analytical methods
- Author
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Bigourd, D., primary, Cuisset, A., additional, Hindle, F., additional, Matton, S., additional, Bocquet, R., additional, Mouret, G., additional, Cazier, F., additional, Dewaele, D., additional, and Nouali, H., additional
- Published
- 2006
- Full Text
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63. Occurrence of Two Metabolic Pathways in Benzo[a]pyrene Degradation by a Deuteromycete Fungus Fusarium solani
- Author
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Rafin, C., primary, Veignie, E., additional, Woisel, P., additional, Cazier, F., additional, and Surpateanu, G., additional
- Published
- 2006
- Full Text
- View/download PDF
64. Pollution photo oxydante et modélisation lagrangienne. campagne de mesures dans le Nord - Pas de Calais
- Author
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Coddeville, P., Guillermo, R., DECHAUX, J. C., ZIMMERMANN, v., Cazier, F., École des Mines de Douai (Mines Douai EMD), Institut Mines-Télécom [Paris] (IMT), Université de Lille, Sciences et Technologies, Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), and Centre commun de mesures (CCM-ULCO)
- Subjects
[SDE]Environmental Sciences ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 1990
65. Synthesis and modification of oxetane based oligomers with 3-ethoxypropylamine by focused microwave irradiation
- Author
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Baudel, V., primary, Cazier, F., additional, Woisel, P., additional, and Surpateanu, G., additional
- Published
- 2002
- Full Text
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66. Metabolization of the Polycyclic Aromatic Hydrocarbon Benzo( A )Pyrene by a Non-White Rot Fungus ( Fusarium Solani ) in a Batch Reactor
- Author
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Veignie, E., primary, Rafin, C., additional, Woisel, P., additional, Sahraoui, A. Lounes-Hadj, additional, and Cazier, F., additional
- Published
- 2002
- Full Text
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67. AN EFFICIENT SYNTHESIS OF 4 -ARYL-4H -[I,2,4]TRIAZOLES UNDER MICROWAVE IRRADIATION IN DRY MEDIA
- Author
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Woisel, P., primary, Cazier, F., additional, Surpateanu, G., additional, Baudel, V., additional, and Boursier, V., additional
- Published
- 2002
- Full Text
- View/download PDF
68. P21-36 Effects of air pollution in asthmatic children: personal exposure to pollutants and biomarkers of effect in exhaled breath condensates.
- Author
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Verdin, A., Migan, M., Cazier, F., Verbrugghe, N., Jaber, N., Dega, A., Kakpo, A., Adonouhoue, L., Sagbo, F., Dewaele, D., Aissi, F., Cachon, B., Patinvoh, U., Agodokpessi, G., Atindehou, C., Fiogbe, A., Lalou, R., and Courcot, D.
- Subjects
- *
BIOMARKERS , *POLLUTANTS , *AIR pollution - Published
- 2024
- Full Text
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69. Élaboration d'une stratégie de lutte contre les oxydants photochimiques et les pluies acides, dépérissement des forêts attribué à la pollution atmosphérique
- Author
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DECHAUX, Jean-Claude, Coddeville, P., ZIMMERMANN, v., Cazier, F., HOUDRET, J.-L., Guillermo, R., Perros, P., Belin, C., PAYEN, M., Université de Lille, Sciences et Technologies, Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Centre commun de mesures (CCM-ULCO), École des Mines de Douai (Mines Douai EMD), Institut Mines-Télécom [Paris] (IMT), laboratoire des acides minéraux, LA79 Montpellier, Laboratoire Interuniversitaire des Systèmes Atmosphériques (LISA (UMR_7583)), and Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[SDE]Environmental Sciences ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience; abstract simple
- Published
- 1988
70. In vitro cytotoxic effects of PM2.5 from the city of Abidjan (Ivory Coast) on human A549 lung cells,Effets cytotoxiques in vitro des PM2,5 de la ville d'Abidjan (Côte-d'Ivoire) sur des cellules pulmonaires humaines
- Author
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Kouassi, K. -S, Billet, S., Garçon, G., Verdini, A., Diouf, A., Cazier, F., Djaman, J., Dominique Courcot, and Shirali, P.
71. Effect of wood pre-treatment on operating conditions, gaseous and particulate emissions during combustion in a pellet stove - first analytical campaign
- Author
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Genevray, P., Schmidt, G., Cazier, F., Dewaele, D., Vandenbilcke, C., Leyssens, G., Trouvé, G., Tschamber, V., Cornelius Schönnenbeck, Allgaier, O., Labbé, S., Balay, F., Denance, Y., Faivre, E., Le-Dreff, C., and Adam, N.
- Subjects
Biomass ,complex mixtures - Abstract
This work aims at studying the influence of the nature of biomass fuel and its pre-treatment on both emission factors of gaseous and particulate pollutants generated by new domestic heating systems in real use conditions. Indeed, last years, domestic wood heating manufacturers have highly optimised combustion conditions, thanks to new technologies development, to respect the new European legislation proposed in 2022. Furthermore, environmental performance can be improved by modifying biomass properties (type, size, chemical composition, moisture rate, ash content …). Here, the influence of washing pretreatment process was studied on three different wood varieties: fir, beech, oak and one standard pellet (DIN CERTCO). Both untreated and washed woods were combusted in a pellet stove in order to compare the environmental impact of the wood washing process on gaseous and particulate emissions. Fuel properties have slightly changed after washing the different woods and impacts have been observed on particulate and gaseous (NOx, CO, PAH) emissions from washed fuels., Proceedings of the 25th European Biomass Conference and Exhibition, 12-15 June 2017, Stockholm, Sweden, pp. 1737-1739
72. In vitro evaluation of the genotoxicity of polycyclic aromatic hydrocarbonscoated onto airborne PM2.5,Génotoxicité des hydrocarbures aromatiques polycycliques adsorbes à la surface d'un aérosol urbano-industriel (PM 2,5)
- Author
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Billet, S., Imane Abbas, Le Goff, J., Verdi, A., Andre, V., Cazier, F., Sichel, F., Shirali, P., and Garçon, G.
73. Static and unsteady pressure measurements on a 50 degree clipped delta wing at M = 0.9
- Author
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HESS, R., primary, WYNNE, E., additional, and CAZIER, F., additional
- Published
- 1982
- Full Text
- View/download PDF
74. Benzene monitoring using laser difference frequency spectroscopy
- Author
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Chen, W., primary, Cazier, F., additional, Boucher, D., additional, and Tittel, F.K., additional
- Full Text
- View/download PDF
75. Benzene monitoring using laser difference frequency spectroscopy.
- Author
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Chen, W., Cazier, F., Boucher, D., and Tittel, F.K.
- Published
- 2000
- Full Text
- View/download PDF
76. Individual exposure level following indoor and outdoor air pollution exposure in Dakar (Senegal)
- Author
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Dorothée Dewaele, Guillaume Garçon, Fabrice Cazier, Jean-Marc Lo-Guidice, Anne Garat, A. Ndong Ba, Mathilde Cabral, Anthony Verdin, Paul Genevray, Dominique Courcot, J. Thomas, Amadou Diouf, Mamadou Fall, M. Gualtieri, Delphine Allorge, Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV), Université du Littoral Côte d'Opale (ULCO), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Impact de l'environnement chimique sur la santé humaine - ULR 4483 (IMPECS), Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Centre commun de mesures (CCM-ULCO), Ndong Ba, A, Verdin, A, Cazier, F, Garcon, G, Thomas, J, Cabral, M, Dewaele, D, Genevray, P, Garat, A, Allorge, D, Diouf, A, Loguidice, J, Courcot, D, Fall, M, and Gualtieri, M
- Subjects
Rural Population ,Urban Population ,010504 meteorology & atmospheric sciences ,Health, Toxicology and Mutagenesis ,Air pollution exposure ,Air pollution ,010501 environmental sciences ,Toxicology ,medicine.disease_cause ,01 natural sciences ,Incense ,Human health ,Exposure level ,Urinary levels ,Personal exposure evaluation ,11. Sustainability ,Indoor and outdoor air pollution exposure ,Cooking ,Volatile organic compounds exposure ,Air Pollutants ,Carbon Monoxide ,General Medicine ,Pollution ,Senegal ,3. Good health ,8-Hydroxy-2'-Deoxyguanosine ,Air Pollution, Indoor ,[SDE]Environmental Sciences ,Environmental Monitoring ,Nitric Oxide ,Biomarkers of exposure ,Environmental health ,medicine ,Humans ,Air quality index ,0105 earth and related environmental sciences ,Pollutant ,Volatile Organic Compounds ,Interleukin-6 ,Interleukin-8 ,Deoxyguanosine ,Benzene ,Environmental Exposure ,Carbon Dioxide ,Oxidative Stress ,Cross-Sectional Studies ,13. Climate action ,Environmental science ,Particulate Matter ,DNA Damage - Abstract
International audience; The consequences of indoor and outdoor air pollution on human health are of great concern nowadays. In this study, we firstly evaluated indoor and outdoor air pollution levels (CO, CO2, NO, NO2, PM10) at an urban site in Dakar city center and at a rural site. Then, the individual exposure levels to selected pollutants and the variations in the levels of biomarkers of exposure were investigated in different groups of persons (bus drivers, traders working along the main roads and housemaids). Benzene exposure levels were higher for housemaids than for bus drivers and traders. High indoor exposure to benzene is probably due to cooking habits (cooking with charcoal), local practices (burning of incense), the use of cleaning products or solvent products which are important emitters of this compound. These results are confirmed by the values of S-PMA, which were higher in housemaids group compared to the others. Urinary 1-HOP levels were significantly higher for urban site housemaids compared to semirural district ones. Moreover, urinary levels of DNA oxidative stress damage (8-OHdG) and inflammatory (interleukin-6 and -8) biomarkers were higher in urban subjects in comparison to rural ones. The air quality measurement campaign showed that the bus interior was more polluted with PM10, CO, CO2 and NO than the market and urban or rural households. However, the interior of households showed higher concentration of VOCs than outdoor sites confirming previous observations of higher indoor individual exposure level to specific classes of pollutants.
- Published
- 2019
77. Physico-chemical characterization and in vitro inflammatory and oxidative potency of atmospheric particles collected in Dakar city's (Senegal)
- Author
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Awa Ndong Ba, Anthony Verdin, Guillaume Garçon, Mamadou Fall, Amadou Diouf, Maurizio Gualtieri, Mathilde Cabral, Fabrice Cazier, Lucie Courcot, Dominique Courcot, Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV), Université du Littoral Côte d'Opale (ULCO), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Impact de l'environnement chimique sur la santé humaine - ULR 4483 (IMPECS), Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 (LOG), Centre National de la Recherche Scientifique (CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut national des sciences de l'Univers (INSU - CNRS), Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Centre commun de mesures (CCM-ULCO), Institut national des sciences de l'Univers (INSU - CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Nord]), Ndong Ba, A, Cazier, F, Verdin, A, Garcon, G, Cabral, M, Courcot, L, Diouf, A, Courcot, D, Gualtieri, M, and Fall, M
- Subjects
25%22">PM>25 ,PM ,010504 meteorology & atmospheric sciences ,Health, Toxicology and Mutagenesis ,Inflammatory response ,Air pollution ,Oxidative phosphorylation ,010501 environmental sciences ,Toxicology ,medicine.disease_cause ,Oxidative damages ,01 natural sciences ,> ,Cell Line ,BEAS-2B cells ,BEAS-2B cell ,Oxidative damage ,11. Sustainability ,DNA adduct ,medicine ,Humans ,Potency ,Particle Size ,Polycyclic Aromatic Hydrocarbons ,Vehicle Emissions ,0105 earth and related environmental sciences ,Pollutant ,Air Pollutants ,Chemistry ,General Medicine ,Pollution ,Senegal ,3. Good health ,Africa, Western ,Oxidative Stress ,West african ,Metals ,2.5 ,13. Climate action ,Environmental chemistry ,Atmospheric pollutants ,[SDE]Environmental Sciences ,Cytokines ,PM 2.5 ,Particulate Matter ,Physicochemical characterization ,Oxidation-Reduction - Abstract
Exposure to atmospheric pollutants has been recognized as a major risk factor of respiratory and cardiovascular diseases. Fine particles (PM2.5) and a coarser fraction (PM>2.5) sampled at an urban site in Dakar (HLM), characterized by high road traffic emissions, were compared with particles sampled at a rural area, Toubab Dialaw located about 40 km from Dakar. The physicochemical characteristics of samples revealed that PMs differ for their physical (surface area) and chemical properties (in terms of CHN, metals, ions, paraffins, VOCs and PAHs) that were 65–75% higher in urban samples. Moreover the fine PMs contain higher amounts of anthropogenic related pollutants than the PM>2.5 one. These differences are sustained by the ratios reported for the analysed PAHs which suggest as predominant primary emission sources vehicle exhausts at urban site and biomass combustion at the rural site. The inflammatory response and the oxidative damages were evaluated in BEAS-2B cells by the quantification of 4 selected inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-8) and of total carbonylated proteins and the oxidative DNA adduct 8-OHdG after 8 or 24 h exposure. In accordance with the different sources and different physical and chemical properties, the inflammatory response and the oxidative damages were found higher in bronchial cells exposed to urban PMs. These data confirm the importance, also for West African countries, to evaluate the correlation between PM physico-chemical properties and potential biological impacts. Dakar air pollution, influenced by anthropogenic activities, causes inflammatory responses and oxidative damages in vitro.
- Published
- 2019
78. Comparative toxicity of air pollution particulate matter (PM2.5) sampled under urban, industrial or rural influence in human bronchial epithelial lung cells (BEAS-2B)
- Author
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Garcon, G., Dergham, M., Billet, S., Lepers, C., Verdin, A., Courcot, D., Cazier, F., and Shirali, P.
- Published
- 2011
- Full Text
- View/download PDF
79. Low cost and renewable H 2 S-biofilter inoculated with Trichoderma harzianum .
- Author
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Chaghouri M, Gennequin C, Tidahy LH, Cazier F, Abi-Aad E, Veignie E, and Rafin C
- Subjects
- Biofuels, Sulfur, Hydrogen Sulfide chemistry, Hydrogen Sulfide metabolism, Hypocreales metabolism
- Abstract
The use of biogas to produce hydrogen is currently gaining more attention. One of the drawbacks for the valorization of biogas is the presence of H
2 S, a hazardous molecule that can cause damage in the metallic internal structures of industries. In this study, the H2 S-removal performance of a fungi-based biofilter was investigated. First, an H2 S-resistant fungal species was isolated from an industrial digestate and identified as Trichoderma harzianum . The capacity of this microorganism to metabolize H2 S in a mineral medium was confirmed. Then, a bioreactor was constructed and put in place to monitor the elimination of gaseous H2 S. A mix of cardboard, perlite, woodchips, and wood pellets was used as filling. Microbial development and the outlet gas composition were monitored during a 60-day experimental process during which H2 S was completely removed. 97% of the introduced sulphur was detected in the used filling material (fungal species + packing material) by elemental analysis. 24% of the detected sulphur was identified by ion-exchange chromatography as SO4 2- . Elemental analysis, gas chromatography, and ion-exchange chromatography were used to determine the bioreactor sulphur balance. Metagenomic analysis underlined that H2 S elimination was due to the presence of Trichoderma harzianum with a H2 S-specific bacterial consortium.- Published
- 2024
- Full Text
- View/download PDF
80. Road-traffic-related air pollution contributes to skin barrier alteration and growth defect of sensory neurons.
- Author
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Le Gall-Lanotto C, Verdin A, Cazier F, Bataille-Savattier A, Guéré C, Dorr MM, Fluhr JW, Courcot D, Vié K, and Misery L
- Subjects
- Rats, Animals, Humans, Particulate Matter toxicity, Skin metabolism, Sensory Receptor Cells, Air Pollutants toxicity, Air Pollution
- Abstract
The effects of air pollution on health are gaining increasing research interest with limited data on skin alterations available. It was suggested that air pollution is a trigger factor for sensitive skin (SS). However, this data was based on surveys with a lack of experimental data. SS is related to altered skin nerve endings and cutaneous neurogenic inflammation. TTe present study was to assess the in vitro effect of particulate matter (PM) on epidermis and nerve ending homeostasis. PM samples were collected according to a validated protocol. Reconstructed human epidermis (RHE, Episkin®) was exposed to PM and subsequently the supernatants were transferred to a culture of PC12 cells differentiated into sensory neurons (SN). Cell viability, axonal growth and neuropeptide-release were measured. The modulation of the expression of different inflammatory, keratinocytes differentiation and neurites growth markers was assessed. PM samples contained a high proportion of particles with a size below 1 μm and a complex chemical composition. Transcriptomic and immunohistochemical analyses revealed that PM altered keratinocytes terminal differentiation and induced an inflammatory response. While viability and functionality of the SN were not modified, their outgrowth was significantly decreased after incubation with PM-exposed Episkin® supernatants. This was closely related to the modification of nerve growth factor/semaphorin 3A balance. This study showed that air pollutants have negative effects on keratinocytes and sensory nerve endings including inflammatory responses. These effects are probably involved in the SS pathophysiology and might be involved in inflammatory skin disorders., (© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2024
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- View/download PDF
81. Acute and repeated exposures of normal human bronchial epithelial (NHBE) cells culture to particles from a coloured pyrotechnic smoke.
- Author
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de Lagarde VM, Chevalier L, Méausoone C, Cazier F, Dewaele D, Cazier-Dennin F, Janona M, Logie C, Achard S, André V, Rogez-Florent T, Monteil C, and Corbiere C
- Subjects
- Humans, Bronchi, Epithelial Cells, Tobacco Products, Cell Culture Techniques, Smoke adverse effects
- Abstract
Coloured pyrotechnic smokes are frequently used in the military field and occasionally by civilians, but their health hazards have been little studied. The main concern could rise from inhalation of smoke particles. Our previous study showed that acute exposure to particles from a red signalling smoke (RSS) induced an antioxidant and inflammatory responses in small airway epithelial cells. The aim of this study was to further explore the toxicity of RSS particles at a more proximal level of the respiratory tract, using normal human bronchial epithelial cells grown at the Air-Liquid Interface. Acute exposure (24 h) induced an oxidative stress that persisted 24 h post-exposure, associated with particle internalization and epithelium morphological changes (cuboidal appearance and loss of cilia). Repeated exposures (4×16h) to RSS particles did not trigger oxidative stress but cell morphological changes occurred. Overall, this study provides a better overview of the toxic effects of coloured smoke particles., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
82. Oxidative stress response in pulmonary cells exposed to different fractions of PM 2.5-0.3 from urban, traffic and industrial sites.
- Author
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Moufarrej L, Verdin A, Cazier F, Ledoux F, and Courcot D
- Subjects
- Reactive Oxygen Species metabolism, Oxidative Stress, Lung metabolism, 8-Hydroxy-2'-Deoxyguanosine, Particulate Matter analysis, Air Pollutants toxicity, Air Pollutants analysis
- Abstract
The aim of this work was to study the relationship between oxidative stress damages and particulate matter (PM) chemical composition, sources, and PM fractions. PM
2.5-0.3 (PM with equivalent aerodynamic diameter between 2.5 and 0.3 μm) were collected at urban, road traffic and industrial sites in the North of France, and were characterized for major and minor chemical species. Four different fractions (whole PM2.5-0.3 , organic, water-soluble and non-extractable matter) were considered for each of the PM2.5-0.3 samples from the three sites. After exposure of BEAS-2B cells to the four different fractions, oxidative stress was studied in cells by quantifying reactive oxygen species (ROS) accumulation, oxidative damage to proteins (carbonylated proteins), membrane alteration (8-isoprostane) and DNA damages (8-OHdG). Whole PM2.5-0.3 was capable of inducing ROS overproduction and caused damage to proteins at higher levels than other fractions. Stronger cell membrane and DNA damages were found associated with PM and organic fractions from the urban site. ROS overproduction was correlated with level of expression of carbonylated proteins, DNA damages and membrane alteration markers. The PM2.5-0.3 collected under industrial influence appears to be the less linked to cell damages and ROS production in comparison with the other influences., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Inc. All rights reserved.)- Published
- 2023
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83. Oxidative potential and in vitro toxicity of particles generated by pyrotechnic smokes in human small airway epithelial cells.
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Martin de Lagarde V, Rogez-Florent T, Cazier F, Dewaele D, Cazier-Dennin F, Ollivier A, Janona M, Achard S, André V, Monteil C, and Corbière C
- Subjects
- Epithelial Cells, Humans, Oxidative Stress, Smoke adverse effects, Smoke analysis, Smoking, Air Pollutants toxicity, Antioxidants
- Abstract
Pyrotechnic smokes are widely used in civilian and military applications. The major issue arise from the release of particles after smoke combustion but the health risks related to their exposure are poorly documented whereas toxicity of airborne particles on the respiratory target are very well known. Therefore, this study aimed to explore the in vitro toxicity of the particle fraction of different pyrotechnic smokes. Particles from a red signalling smoke (RSS), an hexachloroethane-based obscuring smoke (HC-OS) and an anti-intrusion smoke (AIS) were collected from the cloud. RSS particles displayed the highest organic fraction (quinones and polycyclic aromatic hydrocarbons) of the three samples characterized. AIS particles contained K and cholesterol derivatives. HC-OS particles were mainly metallic with very high concentrations of Al, Fe and Ca. Intrinsic oxidative potential of smoke particles was measured with two assays. Depletions of DTT by RSS particles was greater than depletion obtained with AIS and HC-OS particles but depletion of acid ascorbic (AA) was only observed with HC-OS particles. In vitro toxicity was assessed by exposing human small airway epithelial cells (SAEC) to various concentrations of particles. After 24 h of exposure, cell viability was not affected but significant modifications of mRNA expression of antioxidant (SOD-1 and -2, catalase, HO-1, NQO-1) and inflammatory markers (IL-6, IL-8, TNF-α) were observed and were dependent on smoke type. Particles rich in metal, such as HC-OS, induced a greatest depletion of AA and a greatest inflammatory response, whereas particles rich in organic compounds, such as RSS, induced a greatest DTT depletion and a greatest antioxidant response. In conclusion, the three smoke particles have an intrinsic oxidative potential and triggered a cell adaptive response. Our study improved the knowledge of particle toxicity of pyrotechnic smokes and scientific approach developed here could be used to study other type of particles., (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2022
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84. Toxicological responses of BEAS-2B cells to repeated exposures to benzene, toluene, m-xylene, and mesitylene using air-liquid interface method.
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Méausoone C, Landkocz Y, Cazier F, Seigneur M, Courcot D, and Billet S
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- Benzene administration & dosage, Benzene Derivatives administration & dosage, Blotting, Western, Bronchi cytology, Cell Line, Gene Expression drug effects, Humans, Inflammation chemically induced, Respiratory Mucosa cytology, Respiratory Mucosa drug effects, Toluene administration & dosage, Xylenes administration & dosage, Benzene toxicity, Benzene Derivatives toxicity, Bronchi drug effects, Toluene toxicity, Xylenes toxicity
- Abstract
In order to reduce exposure to toxic chemicals, the European REACH regulation (1907/2006) recommends substituting toxic molecules with compounds that are less harmful to human health and the environment. Toluene is one of the most frequently used solvents in industries despite its toxicity. The objective of this study is to better understand and compare the toxicity of toluene and its homologues in a bronchial cell model. Thus, human bronchial BEAS-2B cells were exposed to steams of toluene, m-xylene, mesitylene (1,3,5-trimethylbenzene), and benzene (20 and 100 ppm). Exposure was carried out using an air-liquid interface (ALI) system (Vitrocell) during 1 h/day for 1, 3, or 5 days. Cytotoxicity, xenobiotic metabolism enzyme gene expression, and inflammatory response were evaluated following cell exposures. BEAS-2B cell exposure to toluene and its homologues revealed the involvement of major (CYP2E1) and minor metabolic pathways (CYP1A1). A late induction of genes (EPHX1, DHDH, ALDH2, and ALDH3B1) was measured from Day 3 and can be linked to the formation of metabolites. An increase in the secretion level of inflammatory markers (TNF-α, IL-6, IL-8, MCP-1, and GM-CSF) was also observed. In parallel, regulation between inflammatory mediators and the expression of transmembrane glycoprotein mucin MUC1 was also studied. This in vitro approach with ALI system points out the relevance of conducting repeated exposures to detect potential late effects. The difference recorded after cell exposure to toluene and its homologues highlights the importance of substitution principle., (© 2020 John Wiley & Sons, Ltd.)
- Published
- 2021
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85. Influence of the environmental relative humidity on the inflammatory response of skin model after exposure to various environmental pollutants.
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Seurat E, Verdin A, Cazier F, Courcot D, Fitoussi R, Vié K, Desauziers V, Momas I, Seta N, and Achard S
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- Humans, Humidity, Particulate Matter analysis, Particulate Matter toxicity, Air Pollutants analysis, Air Pollutants toxicity, Environmental Pollutants toxicity, Volatile Organic Compounds analysis, Volatile Organic Compounds toxicity
- Abstract
The skin is an essential barrier, protecting the body against the environment and its numerous pollutants. Several environmental pollutants are known to affect the skin, inducing premature aging through mechanisms including oxidative stress, inflammation, and impairment of skin functions. Even climate conditions can impact the skin. Therefore, using a Reconstructed Human Epidermis (RHE), we tested the effect of two samples of fine particulate matters (PM
0.3-2.5 - one metals-rich sample and the other organic compounds-rich), two Volatile Organic Compounds mixtures (VOCs - from a solvent-based paint and a water-based paint) and Tobacco Smoke (TS). All pollutants affected cellular functionality, but to a lesser extent for the water-based paint VOC. This effect was enhanced when RHE were preconditioned for 2 h by a semi-dry airflow (45% relative humidity) before pollutants application, compared to preconditioning by a humid airflow (90% relative humidity). In the absence of preconditioning, IL-1α, IL-6, IL-8, and RANTES were almost systematically induced by pollutants. When RHE were preconditioned by a semi-dry or humid airflow before being subjected to pollutants, the increase of IL-1α, IL-8, and RANTES falls into two groups. Similarly to RHE not treated with pollutants, RHE treated with VOCs after preconditioning by a semi-dry airflow showed increased IL-1α, IL-8, and RANTES release. On the contrary, RHE treated with PM or TS after preconditioning by a semi-dry airflow show a lower increase in IL-1α, IL-8, and RANTES compared to preconditioning by a humid airflow. The effect of real environmental relative humidity conditions of the air, combined with acute exposure to various environmental pollutants, seemed to relate mainly to structural changes of the skin, determining the outcome of the inflammatory response depending on the physicochemical characteristics of pollutants., (Copyright © 2020 Elsevier Inc. All rights reserved.)- Published
- 2021
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86. Renal impairment assessment on adults living nearby a landfill: Early kidney dysfunction biomarkers linked to the environmental exposure to heavy metals.
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Cabral M, Garçon G, Touré A, Bah F, Dewaele D, Bouhsina S, Cazier F, Faye A, Fall M, Courcot D, and Verdin A
- Abstract
The aim of this study was to assess the integrity and kidney overall functional capacity of subjects exposed to landfill emissions. Urine and blood levels of Pb and Cd, and several of the newly biomarkers of nephrotoxicity (Kim Injury Molecule 1 (KIM-1), alpha-1 Microglobulin (α1 M), beta-2 Microglobulin (β2 M), Cystatin-C (Cyst C), Clusterin, alpha-glutathione S-transferase (GSTα), pi-glutathione S-transferase (GSTπ), Tissue Inhibitor of Metalloproteinase-1 (TIMP1), Calbindin, Neutrophil Gelatinase-Associated Lipocalin (NGAL), Osteopontin (OPN), (Retinol Binding Protein(RBP), Liver-type Fatty Acid-Binding Protein (FABP-1), Trefoil Factor 3 (TFF3), Collagen VI) were measured in order to assess glomerular and tubule damage in adults living near a landfill. Our results indicate glomerular dysfunction in exposed subjects, and supported evidence of necrosis of proximal and distal tubule epithelial cells as specific biomarkers began to appear in the urine. Positive correlation by Pearson test were obtained between : blood Pb and B-OPN, B-Cyst C, Calbindin, U-KIM-1, TIMP1, U-OPN, and U-Clusterin; and also, between urinary Cd and TIMP1, B-Clusterin, U-OPN, FABP-1, Albumin, and U-Clusterin. The relation between biomarkers of Cd/Pb exposure and early effect biomarkers in this study clearly predicts the future risk of severe kidney injury in subjects living close to the landfill., Competing Interests: The authors report no declarations of interest., (© 2021 Published by Elsevier B.V.)
- Published
- 2021
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87. Comparative study on gene expression profile in rat lung after repeated exposure to diesel and biodiesel exhausts upstream and downstream of a particle filter.
- Author
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Lecureur V, Monteil C, Jaguin M, Cazier F, Preterre D, Corbière C, Gosset P, Douki T, Sichel F, and Fardel O
- Subjects
- Animals, Gasoline analysis, Particulate Matter analysis, Rats, Transcriptome, Vehicle Emissions analysis, Air Pollutants analysis, Biofuels analysis
- Abstract
Biodiesel is considered as a valuable and less toxic alternative to diesel. However, cellular and molecular effects of repeated exposure to biodiesel emissions from a recent engine equipped with a diesel particle filter (DPF) remain to be characterized. To gain insights about this point, the lung transcriptional signatures were analyzed for rats (n = 6 per group) exposed to filtered air, 30% rapeseed biodiesel (B30) blend or reference diesel (RF0), upstream and downstream a DPF, for 3 weeks (3 h/day, 5 days/week). Genomic analysis revealed a modest regulation of gene expression level (lower than a 2-fold) by both fuels and a higher number of genes regulated downstream the DPF than upstream, in response to either RF0 or to B30 exhaust emissions. The presence of DPF was found to notably impact the lung gene signature of rats exposed to B30. The number of genes regulated in common by both fuels was low, which is likely due to differences in concentrations of regulated pollutants in exhausts, notably for compound organic volatiles, polycyclic aromatic hydrocarbons, NO or NOx. Nevertheless, we have identified some pathways that were activated for both exhaust emissions, such as integrin-, IGF-1- and Rac-signaling pathways, likely reflecting the effects of gas phase products. By contrast, some canonical pathways relative to "oxidative phosphorylation" and "mitochondrial dysfunction" appear as specific to B30 exhaust emission; the repression of transcripts of mitochondrial respiratory chain in lung of rats exposed to B30 downstream of DPF supports the perturbation of mitochondria function. This study done with a recent diesel engine (compliant with the European IV emission standard) and commercially-available fuels reveals that the diesel blend composition and the presence of an after treatment system may modify lung gene signature of rats repeatedly exposed to exhaust emissions, however in a rather modest manner., Competing Interests: Declaration of competing interest The authors have no conflict to interest to declare., (Copyright © 2020. Published by Elsevier Ltd.)
- Published
- 2020
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88. Toxicological appraisal of the chemical fractions of ambient fine (PM 2.5-0.3 ) and quasi-ultrafine (PM 0.3 ) particles in human bronchial epithelial BEAS-2B cells.
- Author
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Badran G, Verdin A, Grare C, Abbas I, Achour D, Ledoux F, Roumie M, Cazier F, Courcot D, Lo Guidice JM, and Garçon G
- Subjects
- Bronchi, Epithelial Cells, Humans, Organic Chemicals, Oxidative Stress, Particulate Matter analysis, Air Pollutants analysis
- Abstract
New toxicological research is still urgently needed to improve the current knowledge about the induction of some underlying mechanisms of toxicity by the different chemical fractions of ambient particulate matter (PM). This in vitro study sought also to better evaluate and compare the respective toxicities of fine particles (PM
2.5-0.3 ) and their inorganic and organic chemical fractions, and the respective toxicities of the organic chemical fractions of PM2.5-0.3 and quasi-ultrafine particles (PM0.3 ). Human bronchial epithelial BEAS-2B cells were also exposed for 6-48 h to relatively low doses of PM2.5-0.3 and their organic extractable (OEM2.5-0.3 ) and non-extractable (NEM2.5-0.3 ) fractions, and the organic extractable fraction (OEM0.3 ) of PM0.3 . We reported that not only PM2.5-0.3 , but also, to a lesser extent, its inorganic chemical fraction, NEM2.5-0.3 , and organic chemical fraction, OEM2.5-0.3 , were able to significantly induce ROS overproduction and oxidative damage notwithstanding the early activation of NRF2 signaling pathway. Moreover, for any exposure, inflammatory and apoptotic events were noticed. Similar results were observed in BEAS-2B cells exposed to OEM0.3 , rich of polycyclic aromatic hydrocarbons and their nitrated and oxygenated derivatives. In BEAS-2B cells exposed for 24 and 48 h to OEM2.5-0.3 and OEM0.3 , to a higher extent, there was an alteration of the levels of some critical proteins even though crucial for the autophagy rather than a real reduction of autophagy. It is noteworthy that the toxicological effects were equal or mostly higher in BEAS-2B cells exposed for 6 and/or 24 h to PM2.5-0.3 from those exposed to NEM2.5-0.3 or OEM2.5-0.3 , and in BEAS-2B cells exposed for 6 and/or mostly 24 h to OEM0.3 from those exposed to OEM2.5-0.3 . Taken together, these results revealed the higher potentials for toxicity, closely linked to their respective physical and chemical characteristics, of PM2.5-0.3 vs NEM2.5-0.3 and/or OEM2.5-0.3 , and OEM0.3 vs OEM2.5-0.3 ., Competing Interests: Declaration of interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)- Published
- 2020
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89. A prospective pilot study of the T-lymphocyte response to fine particulate matter exposure.
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Al Zallouha M, Landkocz Y, Méausoone C, Ledoux F, Visade F, Cazier F, Martin PJ, Borgie M, Vitagliano JJ, Trémolet G, Cailliez JC, Gosset P, Courcot D, and Billet S
- Subjects
- Activation, Metabolic, Adult, Age Factors, Aged, Aged, 80 and over, Cytochrome P-450 CYP1A1 genetics, Cytochrome P-450 CYP1A1 metabolism, Cytochrome P-450 CYP1B1 genetics, Cytochrome P-450 CYP1B1 metabolism, Cytochrome P-450 Enzyme System genetics, Cytochrome P-450 Enzyme System metabolism, Cytokines metabolism, Female, Gene Expression Regulation, Enzymologic, Humans, Male, Middle Aged, Oxidative Stress drug effects, Particle Size, Pilot Projects, Prospective Studies, T-Lymphocytes immunology, T-Lymphocytes metabolism, Young Adult, Air Pollutants toxicity, Particulate Matter toxicity, T-Lymphocytes drug effects
- Abstract
Exposure to air pollution is associated with increased morbidity and mortality. Once the fine atmospheric particulate matter (FP) is inhaled, some of its compounds can pass through the lungs and reach the bloodstream where they can come into contact with immune cells. Exposure to FP particularly affects sensitive populations such as the elderly. Aging affects the immune system, making the elderly more vulnerable. The project aims to determine the effects of FP exposure on human T cells while looking for biomarkers associated with exposure. Blood samples from 95 healthy subjects in three different age groups (20-30, 45-55 and 70-85 years) were collected to determine a potential age effect. T lymphocytes were isolated to be exposed ex vivo for 72 hours to 45 μg/mL of FP collected in Dunkirk and chemically characterized. Overexpression of the CYP1A1, CYP1B1 and CYP2S1 genes was therefore measured after exposure of the T cells to FP. These genes code for enzymes known to be involved in the metabolic activation of organic compounds such as polycyclic aromatic hydrocarbons detected in the FP sample. T-cell profiling allowed us to suggest a mixed T-helper 1/2 profile caused by exposure to FP. With regard to the influence of age, we have observed differences in the expression of certain genes, as well as an increase in interleukin-4 and -13 concentrations in the elderly. These results showed that exposure of T lymphocytes to FP causes effects on both transcriptomic and cytokine secretion levels., (© 2020 John Wiley & Sons, Ltd.)
- Published
- 2020
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90. Cellular response and extracellular vesicles characterization of human macrophages exposed to fine atmospheric particulate matter.
- Author
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Martin PJ, Héliot A, Trémolet G, Landkocz Y, Dewaele D, Cazier F, Ledoux F, and Courcot D
- Subjects
- Air Pollutants metabolism, Cell Line, Cytokines metabolism, Gene Expression drug effects, Humans, Lung metabolism, Lung Diseases chemically induced, Oxidative Stress, Particle Size, Particulate Matter metabolism, Tumor Necrosis Factor-alpha metabolism, Air Pollutants toxicity, Epithelial Cells metabolism, Extracellular Vesicles metabolism, Macrophages, Alveolar metabolism, Particulate Matter toxicity
- Abstract
Exposure to fine atmospheric Particulate Matter (PM) is one of the major environmental causes involved in the development of inflammatory lung diseases, such as chronic obstructive pulmonary disease (COPD) or asthma. When PM is penetrating in the pulmonary system, alveolar macrophages represent the first line of defense, in particular by triggering a pro-inflammatory response, and also by their ability to recruit infiltrating macrophages from the bone marrow. The aim of this in vitro study was to evaluate the gene expression and cytokine production involved in the toxicological and inflammatory responses of infiltrating macrophages, as well as the Extracellular Vesicles (EVs) production, after their exposure to PM. The ability of these EVs to convey information related to PM exposure from exposed macrophages to pulmonary epithelial cells was also evaluated. Infiltrating macrophages respond to fine particles exposure in a conventional manner, as their exposure to PM induced the expression of Xenobiotic Metabolizing Enzymes (XMEs) such as CYP1A1 and CYP1B1, the enzymes involved in oxidative stress SOD2, NQO1 and HMOX as well as pro-inflammatory cytokines in a dose-dependent manner. Exposure to PM also induced a greater release of EVs in a dose-dependent manner. In addition, the produced EVs were able to induce a pro-inflammatory phenotype on pulmonary epithelial cells, with the induction of the release of IL6 and TNFα proinflammatory cytokines. These results suggest that infiltrating macrophages participate in the pro-inflammatory response induced by PM exposure and that EVs could be involved in this mechanism., (Copyright © 2019 Elsevier Ltd. All rights reserved.)
- Published
- 2019
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91. In vitro toxicological evaluation of emissions from catalytic oxidation removal of industrial VOCs by air/liquid interface (ALI) exposure system in repeated mode.
- Author
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Méausoone C, El Khawaja R, Tremolet G, Siffert S, Cousin R, Cazier F, Billet S, Courcot D, and Landkocz Y
- Subjects
- Air Pollutants chemistry, Aluminum Oxide chemistry, Catalysis, Cell Culture Techniques, Cell Line, Cell Survival drug effects, Cobalt chemistry, Cytochrome P-450 Enzyme System genetics, Gene Expression Regulation drug effects, Humans, Industrial Waste, Interleukin-6 metabolism, Interleukin-8 metabolism, Oxidation-Reduction, Toluene chemistry, Volatile Organic Compounds chemistry, Volatile Organic Compounds toxicity, Air Pollutants toxicity, Toluene toxicity
- Abstract
Toxicity of toluene and by-products formed during its catalytic oxidative degradation was studied in human bronchial BEAS-2B cells repeatedly exposed. BEAS-2B cells were exposed using an Air-Liquid Interface (ALI) System (Vitrocell®) for 1 h per day during 1, 3 or 5 days to gaseous flows: toluene vapors (100 and 1000 ppm) and outflow after catalytic oxidation of toluene (10 and 100%). After exposure to gaseous flow, cytotoxicity, inflammatory response and Xenobiotic Metabolism Enzymes (XME) gene expression were investigated. No significant cytotoxicity was found after 5 days for every condition of exposure. After cells exposure to catalytic oxidation flow, IL-6 level increased no significantly in a time- and dose-dependent way, while an inverted U-shaped profile of IL-8 secretion was observed. XME genes induction, notably CYP2E1 and CYP2F1 results were in line with the presence of unconverted toluene and benzene formed as a by-product, detected by analytical methods. Exposure to pure toluene also demonstrated the activation of these XMEs involved in its metabolism. Repeated exposure permits to show CYP1A1, CYP1B1 and CY2S1 expression, probably related to the formation of other by-products, as PAHs, not detected by standard analytical methods used for the development of catalysts., (Copyright © 2019 Elsevier Ltd. All rights reserved.)
- Published
- 2019
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92. Investigation on the near-field evolution of industrial plumes from metalworking activities.
- Author
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Setyan A, Flament P, Locoge N, Deboudt K, Riffault V, Alleman LY, Schoemaecker C, Arndt J, Augustin P, Healy RM, Wenger JC, Cazier F, Delbarre H, Dewaele D, Dewalle P, Fourmentin M, Genevray P, Gengembre C, Leonardis T, Marris H, and Mbengue S
- Abstract
In a context where a significant fraction of the population lives near industrial areas, the main objectives of this study are to provide (a) new data on PM
2.5 chemical compositions, heavy-metal concentrations and trace gases released by metalworking activities and (b) new information on the near-field evolution (up to about a thousand meters) of such industrial plumes in terms of particle chemical composition and size distribution. For that purpose, a one-month field campaign was performed in an industrial area near the city of Dunkirk (Northern France), combining measurements of atmospheric dynamics and physico-chemical characterization of air masses. Comparisons between several elemental ratios (mainly Mn/Fe), particle size distributions and volatile organic compound (VOC) concentrations at the stacks and at a near-field site suggest that plumes of a ferromanganese alloy plant were quickly mixed with pollutants emitted by other sources (mainly other industries, possibly traffic and sea spray), in particular a neighboring steelworks, before reaching the sampling site. This led to the emergence of secondary particles related to condensation and/or aggregation phenomena inside the plumes. Metalworking emissions were also identified as a source of new particle formation, formed through the emission of gaseous precursors and their fast transformation and condensation, over a timescale of minutes before reaching the near-field site 800 m downwind. Ultrafine particles emitted at the stacks also quickly agglomerated to form larger particles before reaching the near-field site. These results show that, even over short distances, the chemical composition and size distribution of metalworking plumes may evolve rapidly and the characteristics of particles at the boundary of an industrial area (especially in contiguous urban areas) may differ from those emitted directly at the stacks., (Copyright © 2019 Elsevier B.V. All rights reserved.)- Published
- 2019
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93. An in vitro model to evaluate the impact of environmental fine particles (PM 0.3-2.5 ) on skin damage.
- Author
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Verdin A, Cazier F, Fitoussi R, Blanchet N, Vié K, Courcot D, Momas I, Seta N, and Achard S
- Subjects
- Cell Culture Techniques, Cell Survival, Epidermis metabolism, Gene Expression Regulation drug effects, Humans, Keratinocytes drug effects, Oxidative Stress, Particle Size, Toxicity Tests methods, Air Pollutants toxicity, Epidermis drug effects, Keratinocytes physiology, Particulate Matter toxicity
- Abstract
Exposure to airborne particulate matter (PM) has significant effects on human health mainly leading to cardio-respiratory diseases. However very few data are available regarding the impact of PM on the skin, so to better understand the impact of fine particle (PM
0.3-2.5 ) on both inflammatory response and epidermal structure, we exposed a reconstructed human epidermis (RHE) to several doses of PM collected in Cotonou (Benin, West Africa). After 24 h of exposure, inflammatory response, histological observations, and gene expression related to oxidative stress, antioxidant defense and structural damages were determined. No PM-linked changes in tissue morphology or membrane integrity were observable. PM was however cytotoxic in a dose dependent manner. An inflammatory response appeared as shown by the increase in IL-1α and IL-8 cytokine productions. PM also induced oxidative stress, leading to an increase in 4-HNE immunostaining and to the up-regulation of HMOX1, MT1G and MT1E. Finally, PM had a negative impact on fundamental skin functions such as tissue anchorage, cell differentiation, cornification / skin desquamation and apoptosis. Our data show that airborne fine particles have an adverse effect on skin integrity, most probably leading to accelerated ageing., (Copyright © 2019 Elsevier B.V. All rights reserved.)- Published
- 2019
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94. An integrated functional and transcriptomic analysis reveals that repeated exposure to diesel exhaust induces sustained mitochondrial and cardiac dysfunctions.
- Author
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Karoui A, Crochemore C, Mulder P, Preterre D, Cazier F, Dewaele D, Corbière C, Mekki M, Vendeville C, Richard V, Vaugeois JM, Fardel O, Sichel F, Lecureur V, and Monteil C
- Subjects
- Air Pollutants analysis, Air Pollution adverse effects, Air Pollution analysis, Animals, Echocardiography, Male, Mitochondria metabolism, Particulate Matter analysis, Rats, Rats, Wistar, Vehicle Emissions analysis, Air Pollutants toxicity, Cardiovascular System pathology, Mitochondria pathology, Particulate Matter toxicity, Vehicle Emissions toxicity
- Abstract
Diesel exhaust (DE) contributes to air pollution, an important risk factor for cardiovascular diseases. However, the mechanisms by which DE exposure induces cardiovascular dysfunction remain unknown and there is still debate on the contribution of the primary particulate matter (PM) fraction compared to the gaseous phase. Although the mitochondria play a key role in the events leading to cardiovascular diseases, their role in DE-induced cardiovascular effects has not been investigated. The aim of this study was to highlight cardiac and mitochondrial events that could be disrupted following acute and/or repeated DE exposures and the contribution of gaseous pollutants to these effects. To address this question, Wistar rats were exposed to DE generated under strictly controlled and characterized conditions and extracted upstream or downstream of the diesel particulate filter (DPF). Evaluation of the cardiac function after acute DE exposure showed a disturbance in echocardiographic parameters, which persisted and worsened after repeated exposures. The presence of the DPF did not modify the cardiovascular dysfunction revealing an important implication of the gas phase in this response. Surprisingly, redox parameters were not altered by DE exposures while an alteration in mitochondrial oxidative capacity was observed. Exploration of the mitochondrial function demonstrated a more specific alteration in complex I of the respiratory chain after repeated exposures, which was further confirmed by transcriptional analysis of left ventricular (LV) tissue. In conclusion, this work provides new insights into cardiovascular effects induced by DE, demonstrating a cardiac mitochondrial impairment associated with the gaseous phase. These effects suggest deleterious consequences in terms of cardiac function for vulnerable populations with underlying energy deficit such as patients with heart failure or the elderly., (Copyright © 2018 Elsevier Ltd. All rights reserved.)
- Published
- 2019
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95. Physico-chemical characterization and in vitro inflammatory and oxidative potency of atmospheric particles collected in Dakar city's (Senegal).
- Author
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Ndong Ba A, Cazier F, Verdin A, Garcon G, Cabral M, Courcot L, Diouf A, Courcot D, Gualtieri M, and Fall M
- Subjects
- Africa, Western, Air Pollutants analysis, Air Pollution analysis, Cell Line, Humans, Metals adverse effects, Metals analysis, Oxidation-Reduction, Particle Size, Particulate Matter analysis, Polycyclic Aromatic Hydrocarbons adverse effects, Polycyclic Aromatic Hydrocarbons analysis, Senegal, Vehicle Emissions analysis, Air Pollutants adverse effects, Air Pollution adverse effects, Cytokines analysis, Oxidative Stress drug effects, Particulate Matter adverse effects
- Abstract
Exposure to atmospheric pollutants has been recognized as a major risk factor of respiratory and cardiovascular diseases. Fine particles (PM
2.5 ) and a coarser fraction (PM>2.5 ) sampled at an urban site in Dakar (HLM), characterized by high road traffic emissions, were compared with particles sampled at a rural area, Toubab Dialaw located about 40 km from Dakar. The physicochemical characteristics of samples revealed that PMs differ for their physical (surface area) and chemical properties (in terms of CHN, metals, ions, paraffins, VOCs and PAHs) that were 65-75% higher in urban samples. Moreover the fine PMs contain higher amounts of anthropogenic related pollutants than the PM>2.5 one. These differences are sustained by the ratios reported for the analysed PAHs which suggest as predominant primary emission sources vehicle exhausts at urban site and biomass combustion at the rural site. The inflammatory response and the oxidative damages were evaluated in BEAS-2B cells by the quantification of 4 selected inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-8) and of total carbonylated proteins and the oxidative DNA adduct 8-OHdG after 8 or 24 h exposure. In accordance with the different sources and different physical and chemical properties, the inflammatory response and the oxidative damages were found higher in bronchial cells exposed to urban PMs. These data confirm the importance, also for West African countries, to evaluate the correlation between PM physico-chemical properties and potential biological impacts., (Copyright © 2018 Elsevier Ltd. All rights reserved.)- Published
- 2019
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96. Influence of ship emissions on NO x , SO 2 , O 3 and PM concentrations in a North-Sea harbor in France.
- Author
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Ledoux F, Roche C, Cazier F, Beaugard C, and Courcot D
- Subjects
- France, Nitrogen analysis, Oxygen analysis, Ozone analysis, Particle Size, Particulate Matter analysis, Sulfur Dioxide analysis, Air Pollutants analysis, Air Pollution statistics & numerical data, Environmental Monitoring, Ships statistics & numerical data, Vehicle Emissions analysis
- Abstract
The influence of in-port ship emissions on gases and PM
10 concentrations has been estimated in the port city of Calais, northern France, one of the busiest harbor in Europe, with numerous rotations of ferries or roll-on/roll-off cargo in average per day. NOx , SO2 , O3 and PM10 concentrations were continuously measured over a three-month period, as well as real-time particle size distribution. A rural site located at Cape Gris-Nez, 20km from Calais, was considered to deduce intrinsic contribution of ship emissions at the harbor city. The average concentrations of the studied species as well as the pattern of the conditional bivariate probability function at the two sites evidenced that in-port shipping, especially during the maneuvering operations, has an important influence on the NOx and SO2 concentrations. The impact of shipping in the harbor of Calais on average concentrations was estimated to 51% for SO2 , 35% for NO, 15% for NO2 and 2% for PM10 in the studied period. Concentration peaks of SO2 and NOx associated with an O3 depletion appeared synchronized with departures and arrivals of ferries. For winds blowing from the harbor, when compared to the background level, the number of particles appeared 10 times higher, with the highest differences in the 30-67nm and the 109-167nm size ranges. The average impact of in-port ships on PM10 concentrations was estimated to +28.9μg/m3 and concerned mainly the PM1 size fraction (40%). Punctually, PM10 can potentially reach a concentration value close to 100μg/m3 ., (Copyright © 2018. Published by Elsevier B.V.)- Published
- 2018
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97. Chemical characterization of fine and ultrafine PM, direct and indirect genotoxicity of PM and their organic extracts on pulmonary cells.
- Author
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Billet S, Landkocz Y, Martin PJ, Verdin A, Ledoux F, Lepers C, André V, Cazier F, Sichel F, Shirali P, Gosset P, and Courcot D
- Subjects
- Cell Line, Humans, Lung, Air Pollutants toxicity, DNA Damage, Mutagenicity Tests, Particulate Matter toxicity
- Abstract
Particulate matter in ambient air constitutes a complex mixture of fine and ultrafine particles composed of various chemical compounds including metals, ions, and organics. A multidisciplinary approach was developed by studying physico-chemical characteristics and mechanisms involved in the toxicity of particulate atmospheric pollution. PM
0.3-2.5 and PM2.5 including ultrafine particles were sampled in Dunkerque, a French industrialized seaside city. PM samples were characterized from a chemical and toxicological point of view. Physico-chemical characterization evidenced that PM2.5 comes from several sources: natural ones, such as soil resuspension and marine sea-salt emissions, as well as anthropogenic ones, such as shipping traffic, road traffic, and industrial activities. Human BEAS-2B lung cells were exposed to PM0.3-2.5 , or to the Extractable Organic Matter (EOM) of PM0.3-2.5 and PM2.5 . These exposures induced several mechanisms of action implied in the genotoxicity, such as oxidative DNA adducts and DNA Damage Response. The toxicity of PM-EOM was higher for the sample including the ultrafine fraction (PM2.5 ) containing also higher concentrations of polycyclic aromatic hydrocarbons. These results evidenced the major role of organic compounds in the toxicity of PM., (Copyright © 2018. Published by Elsevier B.V.)- Published
- 2018
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98. Fine and ultrafine atmospheric particulate matter at a multi-influenced urban site: Physicochemical characterization, mutagenicity and cytotoxicity.
- Author
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Landkocz Y, Ledoux F, André V, Cazier F, Genevray P, Dewaele D, Martin PJ, Lepers C, Verdin A, Courcot L, Boushina S, Sichel F, Gualtieri M, Shirali P, Courcot D, and Billet S
- Subjects
- Air Pollutants toxicity, Air Pollution analysis, Cities, DNA Damage, Environmental Monitoring, Industry, Mutagenicity Tests, Mutagens toxicity, Particle Size, Particulate Matter toxicity, Photochemical Processes, Polycyclic Aromatic Hydrocarbons analysis, Polycyclic Aromatic Hydrocarbons toxicity, Silicones, Air Pollutants analysis, Particulate Matter analysis
- Abstract
Particulate Matter (PM) air pollution is one of the major concerns for environment and health. Understanding the heterogeneity and complexity of fine and ultrafine PM is a fundamental issue notably for the assessment of PM toxicological effects. The aim of this study was to evaluate mutagenicity and cytotoxicity of a multi-influenced urban site PM, with or without the ultrafine fraction. For this purpose, PM
2.5-0.3 (PM with aerodynamic diameter ranging from 0.3 to 2.5 μm) and PM2.5 were collected in Dunkerque, a French coastal industrial city and were extensively characterized for their physico-chemical properties, including inorganic and organic species. In order to identify the possible sources of atmospheric pollution, specific criteria like Carbon Preference Index (CPI) and PAH characteristic ratios were investigated. Mutagenicity assays using Ames test with TA98, TA102 and YG1041 Salmonella strains with or without S9 activation were performed on native PM sample and PM organic extracts and water-soluble fractions. BEAS-2B cell viability and cell proliferation were evaluated measuring lactate dehydrogenase release and mitochondrial dehydrogenase activity after exposure to PM and their extracts. Several contributing sources were identified in PM: soil resuspension, marine emissions including sea-salt or shipping, road traffic and industrial activities, mainly related to steelmaking or petro-chemistry. Mutagenicity of PM was evidenced, especially for PM2.5 , including ultrafine fraction, in relation to PAHs content and possibly nitro-aromatics compounds. PM induced cytotoxic effects at relatively high doses, while alteration of proliferation with low PM doses could be related to underlying mechanisms such as genotoxicity., (Copyright © 2016 Elsevier Ltd. All rights reserved.)- Published
- 2017
- Full Text
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99. Characterisation and seasonal variations of particles in the atmosphere of rural, urban and industrial areas: Organic compounds.
- Author
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Cazier F, Genevray P, Dewaele D, Nouali H, Verdin A, Ledoux F, Hachimi A, Courcot L, Billet S, Bouhsina S, Shirali P, Garçon G, and Courcot D
- Subjects
- Aerosols analysis, Atmosphere chemistry, Cities, France, Incineration, Industry, Polychlorinated Biphenyls analysis, Polycyclic Aromatic Hydrocarbons analysis, Seasons, Air Pollutants analysis, Environmental Monitoring, Organic Chemicals analysis, Particulate Matter analysis
- Abstract
Atmospheric aerosol samples (PM2.5-0.3, i.e., atmospheric particles ranging from 0.3 to 2.5μm) were collected during two periods: spring-summer 2008 and autumn-winter 2008-2009, using high volume samplers equipped with cascade impactors. Two sites located in the Northern France were compared in this study: a highly industrialised city (Dunkirk) and a rural site (Rubrouck). Physicochemical analysis of particulate matter (PM) was undertaken to propose parameters that could be used to distinguish the various sources and to exhibit seasonal variations but also to provide knowledge of chemical element composition for the interpretation of future toxicological studies. The study showed that PM2.5-0.3 concentration in the atmosphere of the rural area remains stable along the year and was significantly lower than in the urban or industrial ones, for which concentrations increase during winter. High concentrations of polycyclic aromatic hydrocarbons (PAHs), dioxins, furans and dioxin like polychlorinated biphenyls (DL-PCBs), generated by industrial activities, traffic and municipal wastes incineration were detected in the samples. Specific criteria like Carbon Preference Index (CPI) and Combustion PAHs/Total PAHs ratio (CPAHs/TPAHs) were used to identify the possible sources of atmospheric pollution. They revealed that paraffins are mainly emitted by biogenic sources in spring-summer whereas as in the case of PAHs, they have numerous anthropogenic emission sources in autumn-winter (mainly from traffic and domestic heating)., (Copyright © 2016. Published by Elsevier B.V.)
- Published
- 2016
- Full Text
- View/download PDF
100. In vitro short-term exposure to air pollution PM2.5-0.3 induced cell cycle alterations and genetic instability in a human lung cell coculture model.
- Author
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Abbas I, Verdin A, Escande F, Saint-Georges F, Cazier F, Mulliez P, Courcot D, Shirali P, Gosset P, and Garçon G
- Subjects
- Cell Line, Lung drug effects, Particle Size, Air Pollutants toxicity, Apoptosis drug effects, Cell Proliferation drug effects, Gene Expression drug effects, Particulate Matter toxicity, Signal Transduction drug effects
- Abstract
Although its adverse health effects of air pollution particulate matter (PM2.5) are well-documented and often related to oxidative stress and pro-inflammatory response, recent evidence support the role of the remodeling of the airway epithelium involving the regulation of cell death processes. Hence, the overarching goals of the present study were to use an in vitro coculture model, based on human AM and L132 cells to study the possible alteration of TP53-RB gene signaling pathways (i.e. cell cycle phases, gene expression of TP53, BCL2, BAX, P21, CCND1, and RB, and protein concentrations of their active forms), and genetic instability (i.e. LOH and/or MSI) in the PM2.5-0.3-exposed coculture model. PM2.5-0.3 exposure of human AM from the coculture model induced marked cell cycle alterations after 24h, as shown by increased numbers of L132 cells in subG1 and S+G2 cell cycle phases, indicating apoptosis and proliferation. Accordingly, activation of the TP53-RB gene signaling pathways after the coculture model exposure to PM2.5-0.3 was reported in the L132 cells. Exposure of human AM from the coculture model to PM2.5-0.3 resulted in MS alterations in 3p chromosome multiple critical regions in L132 cell population. Hence, in vitro short-term exposure of the coculture model to PM2.5-0.3 induced cell cycle alterations relying on the sequential occurrence of molecular abnormalities from TP53-RB gene signaling pathway activation and genetic instability., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
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