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Póster presentado a la XXI Reunión de la Sociedad Española de Fisiología Vegetal y al XIV Congreso Hispano-luso de Fisiología Vegetal, celebrados en Toledo del 14 al 17 de Junio de 2015.

Here, we provide a detailed protocol describing a SDS-PAGE based procedure to assay in gel neutral lipase activity. Total protein extracts are separated by SDS-PAGE and gels are treated with lipase substrate-α-naphthyl palmitate. This long-chain fatty acid ester is hydrolysed by lipases present in the gel. The product resulting from this reaction can be then visualized in the gel as yellowbrownish activity bands. This relatively simple and effective method of lipase assay detection can be used for crude protein extracts from different plant tissues., This work was supported by the Spanish Ministry of Science and Innovation (MICINN) (ERDFcofinanced project AGL2008-00517) and the Junta de Andalucía (ERDF-cofinanced project P2010-CVI5767).

Trabajo presentado al II Plant Genomics Congress, celebrado en Londres (UK) del 12 al 13 de mayo de 2014.

Resumen del póster presentado al XXIII International Congress on Sexual Plant Reproduction, celebrado en Porto (Portugal) del 13 al 18 de julio de 2014., Organelles called oil bodies (OBs), which are gradually mobilized during pollen germination. Our current knowledge regarding the molecular mechanisms that regulate OB mobilization in the pollen tube is scarce. In this work, we characterized severa! OB-associated enzymes involved in storage lipid breakdown. First, we showed that phospholipase A (PLA) and peroxygenase (PX) proteins were early incorporated during OB biogenesis, whereas acyllipase (LIP) and lipoxygenase (LOX) enzymes were recruited after pollen germination. Drug inhibition of LIP and LOX enzymes hampered both pollen germination and pollen tube growth, leading to a characteristic accumulation pattern of OBs at the germinative aperture. We propase that two independent pathways involving LIP and LOX/PX enzymes, respectively, are responsible for early mobilization of neutrallipids in the pollen tube, while PLAs may promete the access of these enzymes to the OB matrix. Moreover, pollen performance in vitro was not affected under sugar-deprivation conditions but OBassociated enzyme activities were enhanced and OB mobilization was sped up. These data suggest that OBs are sufficient to promote the autonomous growth of the olive pollen tube at the onset of germination, while sugar availability may modulate the activity of these degrading enzymes., This work was funded by MICINN (ERDF-cofinanced projects AGL2008-00517 and BFU2011-22779) and Junta de Andalucía (ERDF-cofinanced project PlO-CVI-5767). KZ and AZ thank the CSIC for providing JAE-DOC grants.

Published by the Plant Reproductive Biology Research Group, Estación Experimental del Zaidín (CSIC), Work subject to a Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0 Internacional License., [EN] This book displays the history of Microscopy at the Estación Experimental del Zaidín (CSIC) in images over the last 35 years through the scientific contributions of those groups that use these techniques for their research. It also intends to be a little tribute to all the people who contributed with his effort and dedication to the development of Microscopy in the institute., [ES] Este libro recoge en imágenes la historia de la Microscopía en la Estación Experimental del Zaidín (CSIC) en los últimos 35 años a través de los trabajos científicos de aquellos grupos que utilizan dichas técnicas en su investigación. Pretende ser además un pequeño homenaje a todas aquellas personas que, con su esfuerzo y dedicación, han contribuido al desarrollo de la Microscopía en el centro.

Proceedings of the I Congress PIIISA celebrado en la Estación Experimental del Zaidín (Granada), en mayo de 2013., Seedless triploid varieties of watermelon (Citrullus lanatus var. lanatus) are very appreciated by consumers, but its production is limited because pollen donor diploid plants and insectassisted pollination is required. To improve this process it is necessary to better study pollen biology aspects such as pollen viability and germinability during long-term storage, stigma receptivity period, etc. In this work we have compared the morphology and ultrastructure of triploid and diploid plant-derived pollen grains in commercial varieties using diverse microscopy techniques. We have not detected at this stage key macroscopic morphological differences between diploid and triploid flowers. Anther development within the triploid flower is highly asynchronous. Microspores from triploid plants are larger than those from diploid plants and showed symptoms of cytoplasmic degeneration. Pollen grains from triploid plants present different morphologies, contain three isodiametric pores but colpi are sometimes not well developed. Moreover, and also depending on the hydration stage, the pollen surface is sometimes smooth, without the characteristic reticulate pattern present in pollen grains from diploid plants. All these developmental features may lead to infertility of triploid plant-derived pollen., This work was supported by ERDF-co-financed projects BFU2011-22779 (Spanish Ministry of Science and Innovation), P2010-AGR-6274, P2010-CV-I5767, and P2011-CVI-7487 (Junta de Andalucía), and RECUPERA2020 3.1.4 (Spanish Ministry of Economy and Competitiveness/ CSIC).

Olías, Raquel et al.-- Scientific editors: Alché Ramírez, Juan de Dios; Belver, Andrés; Castro López, Antonio Jesús; Clemente, Alfonso; Corpas, Francisco J.; Delgado Moreno, Laura; Espinosa-Urgel, Manuel; Jaime, Noelia; Martínez-Abarca, Francisco; Olías, Raquel; Palma Martínez, José Manuel; Rodríguez Serrano, María; Romero, Esperanza; Romero, María C.; Ruiz, Carmelo; Sahrawy, Mariam; Sandalio, Luisa M.; Serrato, Antonio Jesús.-- 109 páginas, con gráficos e imágenes.-- Proceedings of the I Congress PIIISA celebrado en la Estación Experimental del Zaidín (Granada), en mayo de 2013., [EN] Hardly could those around us admit that young people from our High Schools would be able to do top level scientific research. This book demonstrates that, in fact, it is possible. Inside, there are ten papers that summarize the work developed by ten scientific teams with professional researchers and secondary school students at the Estación Experimental del Zaidín (EEZ-CSIC). Once again, our students have had the opportunity of learning about the latest techniques in molecular biology like DNA and protein extraction, DNA amplification using PCR, electrophoresis, gene sequencing, and knowing the most advanced instruments to watch the inner structures of life, as optical and electron microscopes. This is a challenge in which young people can go ahead of their own teachers at their high schools. But PIIISA projects have other advantages as well. These pages include the Proceedings of the III PIIISA-EEZ Congress. Then, our students will have carried out one of the most important features of science: to expose their results to everybody. They are going to learn that science is something meaningless if it cannot be transmited to the society. And moreover, this volume will become their first published paper thanks to the EEZ. And for all of these reasons we, as their teachers, have to thank the EEZ researchers for the important work performed with our students. Because they have been able to accept the challenge of becoming teachers of teenagers, of teaching complex concepts and techniques. And the most important fact: they have succeeded. And in relation to our educative system we can discover new perspectives. At least, in its compulsory levels, it offers a basic formation that lets our students get on in our modern world. But we usually forget that in our hands we also have the scientists of the future, young people who, thanks to projects like PIIISA, are at the forefront and who are going to have a clear view of scientific research much greater that youngsters with the same age. We don’t know if they will study in the future a scientific degree related to the research they have carried out now, but they know that after these experiences, their vision of science will be different, the won’t see science with the same eyes as months ago when they came into the EEZ. But we should not stop here; PIIISA spirit must spread to the rest of the society. PIIISA must be a way that leads to a change in the perception of science in society, a way that definitely launches our country to Science. And this way begins just now, with our students, with researchers, with us, their teachers, with their partners in the classroom, and even though, their families. Without science there is no progress; without progress there is no future. PIIISA shows that all of these objectives are possible. These pages demonstrate it., [ES] Difícilmente aquellos que nos rodean llegarían a pensar que jóvenes de nuestros institutos pudieran ser capaces de llevar a cabo una investigación científica de alto nivel. Este libro demuestra, que de hecho, sí que es posible. Contiene diez artículos científicos que resumen el trabajo llevado a cabo por diez equipos constituidos por investigadores profesionales y por estudiantes de secundaria y bachillerato de institutos granadinos en la Estación Experimental del Zaidín (EEZ-CSIC). Una vez más, nuestros estudiantes han tenido la oportunidad de aprender las últimas técnicas en biología molecular, entre ellas, la extracción de ADN y proteínas, la amplificación de ADN mediante la reacción en cadena de la polimerasa, la secuenciación de genes, o de conocer aquellos otros instrumentos que permiten ver las estructuras más íntimas de la materia viva, como los microscopios ópticos y electrónicos. Es este un reto en el que los jóvenes llegar a ir incluso por delante de nosotros, sus profesores de los centros educativos. Pero PIIISA muestra además otras ventajas. Estas páginas son las Actas del III Congreso PIIISA-EEZ. En ese momento, nuestros estudiantes también habrán llevado a cabo otra de las funciones más importantes de la ciencia, la de exponer los resultados obtenidos. Ellos también aprenderán que la ciencia es algo sin sentido sino se transmite al resto de la sociedad. Y más aún, este volumen contendrá su primer artículo publicado, también gracias a la EEZ. Es por todo ello por lo que, como profesores de estos jóvenes, hemos de reconocer a los investigadores de la Estación Experimental del Zaidín el importante trabajo llevado a cabo con nuestros estudiantes, ya que han sido capaces de aceptar el desafío de convertirse en profesores de adolescentes, de transmitirles técnicas y conceptos complejos. Y lo más importante: han tenido éxito. Y también podemos ver nuevas perspectivas en relación a nuestro sistema educativo. Al menos, en su enseñanza obligatoria, ofrece una formación básica que permite a nuestros alumnado enfrentarse al mundo actual. Pero normalmente olvidamos que también tenemos en nuestras manos a los científicos del futuro, gente joven que, gracias a proyectos como PIIISA, están en la vanguardia y van a tener una visión mucho más clara que otros jóvenes de su edad. Desconocemos si en un futuro ellos estudiarán una carrera relacionada con la investigación que ahora han desarrollado, pero ellos son conscientes de que tras estas experiencias su visión de la ciencia será diferente; no verán la ciencia con los mismos ojos que hace unos meses cuando llegaron a la EEZ. Pero no debemos detenernos aquí. El espíritu PIIISA debe extenderse al resto de la sociedad. PIIISA debe ser un camino que conduzca a un cambio en la percepción de la ciencia en la sociedad, una vía que definitivamente lance a nuestro país hacia la Ciencia. Y este recorrido comienza justo ahora, con nuestros estudiantes, con los investigadores, con nosotros, sus profesores, con sus compañeros de clase y, por supuesto, con sus familias. Sin ciencia no hay progreso; sin progreso no hay futuro. Y PIIISA demuestra que todos estos objetivos son posibles. Estas páginas lo demuestran., Contiene: Can we produce tomatoes with a pinch of salt? (or how to reduce the negative impact of water and soil salinity on cultivated tomato): This work was supported by ERDF-cofinanced grant AGL2010-17090 from the Spanish Ministry of Economy and Competitiveness and N.J-P. was supported by a F.P.I. Program.-- Fructose signalling unbalancing and effect on Arabidopsis growth and the photosynthetic pigments content: This work was supported by “Programa Nacional de Proyectos de Investigación Fundamental” from “Ministerio de Economía y Competitividad” (BIO2012-33292).-- Functional characterization of peroxisomal protein kinases in Arabidopsis plants:This work was supported by ERDF-co-financed grant BIO2012-36742 from MICINN, Junta de Andalucía (BIO-337) and FECYT.-- Garlic used as a preservative increases the reduced form of ascorbate in pepper fruits: This work was supported by the ERDF-cofinanced grant AGL2011-26044 from the Ministry of Science and Innovation, Spain.-- Seedless watermelons: from the microscope to the table through the greenhouse: This work was supported by ERDF-co-financed projects BFU2011-22779 (Spanish Ministry of Science and Innovation), P2010-AGR-6274, P2010-CV-I5767, and P2011-CVI-7487 (Junta de Andalucía), and RECUPERA2020 3.1.4 (Spanish Ministry of Economy and Competitiveness/CSIC).-- Single mutations in a unique gene explain the non-mucoid phenotype in soil bacteria: It was supported mainly by research project MICINN Consolider-Ingenio 2010. CSD2009-00006.-- Use of plant extracts to block bacterial biofilm formation: This work was supported in part by grant BFU2010-17946 from the Plan Nacional de I+D+I. The activities in this project are included in COST action FA1202 (BacFoodNet).-- Polyacrylamide gel electrophoresis: a powerful tool in the food-processing sector: This work was supported by the ERDF-cofinanced grant AGL2011-26044 from the Ministry of Science and Innovation, Spain.-- Internalization of the anti-carcinogenic IBB1, a major Bowman-Birk isoinhibitor from soybean (Glycine max), in HT29 colon cancer cells: This work was supported by ERDF-co-financed grants AGL2011-26353 (Spanish Ministry of Economy and Competitiveness) and PE2010-CVI-5767 (Junta de Andalucía).-- Use of vermitea for removing pesticides from soil: This work was supported by the Spanish Science and Innovation Ministry and co-funded by European funds through the projects CTM2010-16807., Contiene: Can we produce tomatoes with a pinch of salt? (or how to reduce the negative impact of water and soil salinity on cultivated tomato). S. Clemente, Á. Maldonado, P. Vilchez, E. Pérez, A. López, N. Jaime-Pérez, R. Olías, A. Belver.-- Fructose signalling unbalancing and effect on Arabidopsis growth and the photosynthetic pigments content. R. Barnes Ontiveros, M. Fiñaga Cortés, F. López Rodrigo, M. E. Noguera Vilches, C. Rodríguez Ibáñez, M. Sahrawy Barragán, A. J. Serrato Recio.-- Functional characterization of peroxisomal protein kinases in Arabidopsis plants. A.I. Olmedo Moya, A. Sánchez Alonso, G. Beiro Valenzuela, A.C. Villegas Buendia, A. Ollero del Peral, A. Valdivia Ruiz, K.E. Cárdenas, L.M. Sandalio, M. Rodríguez Serrano.-- Garlic used as a preservative increases the reduced form of ascorbate in pepper fruits. C. Aranda López, I. García Fernández-Gaytán, I. Hernández Quero, S. Sánchez Requena, C. Ruiz, F.J. Corpas, J.M. Palma.-- Seedless watermelons: from the microscope to the table through the greenhouse. A.J. Castro, E. García, T. M. Caballero, L. Linares, C. Pérez, A. Piñar, N. Rivas, N. V. Santillán, K. Zienkiewicz, A. Zienkiewicz, M. Jamilena, J.D. Alché.-- Single mutations in a unique gene explain the non-mucoid phenotype in soil bacteria. P.Fernández-Cruz, M. J. Guerrero-Dionisio , M. Lara-García, M. Sánchez-Muñoz, J. L. Sánchez-Segovia, T. Ruiz-Rico, F. Martínez-Abarca.-- Use of plant extracts to block bacterial biofilm formation. J. A. Lizana, S. López, A. Marchal, Ú. Serrano, D. Velasco, M. Espinosa-Urgel.-- Polyacrylamide gel electrophoresis: a powerful tool in the food-processing sector. N. Álvarez Mittelmann, E. Diago Saavedra, E. M. García Calvo, A. Montero Méndez, J. C. Pardo Carmona, S. Pérez López, E. Soriano Calvo, C. Tortosa Jiménez, C. Ruiz, F. J. Corpas, J. M. Palma.-- Internalization of the anti-carcinogenic IBB1, a major Bowman-Birk isoinhibitor from soybean (Glycine max), in HT29 colon cancer cells. M. Soria, P. Porras, F. J. Moya, N. Sánchez, B. Rodríguez, M. L. Ruiz, J. D. Alché, M. C. Arques, A. J. Castro, A. Clemente.-- Use of vermitea for removing pesticides from soil. L. León Rejón, B. García Trigueros, M. Rosales Reyes, C. Soto Paniza, C. Cámara Pérez-Vela, C. Cifuentes, E. Romero, L. Delgado-Moreno.-- The importance of our environment: a qualitative study of water and soil in Iznalloz (Granada, Spain). M. B. Bueno, L. Cuesta, A. Fresneda, J. M. Garrido, C. González, Á. Gutiérrez, P. Morales, M. C. Rivas, M. Ruiz, P. Vílchez, M. Ortiz, C. Plata

Resumen del póster presentado al Pollen Congress, celebrado en Madrid (España) del 17 al 20 de septiembre de 2013., Cell wall pectins and arabinogalactan proteins (AGPs) are important for pollen tube growth. In this study, immunoblot profiling analyses combined with immunocytochemical techniques were used to analyze the temporal and spatial dynamics of four pectin and two AGP epitopes in the olive pollen during in vitro germination. All pectin and AGP epitopes increased their levels during germination. In the mature pollen, galactans localized in the cytoplasm of the vegetative cell, the pollen wall, and the apertural intine. After gennination, bgalactans localized in the pollen tube wall, particularly at the tip, and formed a collar-like structure around the germinative aperture. Arabinans were mainly present in the pollen tube cell wall, forming ring-shaped deposits at regular intervals in the subapical zone. As expected, the pollen tu be wall was rich in highly methyl-esterified pectins at the apex, while the cell wall mainly contained desesterified pectins in the shank. The wall of the generative cell was labeled with arabinans, highly methyl-esterified homogalacturonans and JIM13 epi topes. In addition, the extracelular material coating the outer oxine layer was rich in arabinans, desesterificd pectins and JIM 13 epitopcs. Our results show that pectins and AGPs are newly synthesized in the pollen tu be during pollen germination, being their synthesis and secretion temporally and spatially regulated. We suggest that galactans might provide mechanical stability to the pollen tube, reinforcing those regions that are particularly sensitive to tension stress and/or mechanical damage. On the other hand, arabinans and AGPs might be important in recognition and adhesion phenomena of the pollen tube and the stylar transmitting cclls, as well as the egg and sperm cells., This work was funded by ERDF-cofinanced project AGL2008-00517/AGR (MCINN) and P06-AOR-o1791 (Junta de Andlucia). KZ and AZ thank the CSIC for providing JAE DOCgrants.

Resumen del póster presentado a la 11th International POG Conference: "Reactive Oxygen and Nitrogen Species in Plants", celebrado de 17 al 19 de julio de 2013 en Varsovia (Polonia)., Enzyme activities regulating ROS metabolism in the olive seeds are relatively unknown, in spite of the economical importance of this tree and the increasing interest of these seeds for multiple purposes. The aim of this work was to provide a comprehensive analysis of the genes encoding major antioxidant enzymes in mature seed. For this purpose, we have used transcriptomic sequence information available through our own experiments and in specific databases (Olea EST). Numerous sequences have been retrieved and analysed by using basic descriptive tools. Expression of transcripts encoding several of these enzymes has been carried out. These include catalases, superoxide dismutase, dehydroascorbate reductase, GSNO reductase, NADPH oxidase, lipoxygenase and thioredoxins. Cellular localization of several of these enzymes and histochemical detection of enzyme activities have been also carried out. We discuss how these enzyme activities may be involved in the olive seed metabolism through germination, and how they may contribute to the antioxidant capacity, stability and organoleptic properties of olive oils., This work was supported by ERDF cofunded grants BFU2011-22779, AGL2011-24428, P2010-AGR6274, P2011-CVI7487.

Resumen del póster presentado al XIII Congresso Luso-Espanhol de Fisiologia Vegetal, celebrada del 24 al 28 de julio de 2013 en Lisboa (Portugal)., In oleaginous plants, the mature pollen grain accumulates large amounts of neutral lipids in organelles called oil bodies (OBs). These energy reserves allow the pollen tube to grow autonomously at the early steps of germination. Moreover, the stored lipids can be also the origin of signalling lipids regulating pollen tube growth. Therefore, they undoubtedly represent key factors for the success of pollination. This fact contrasts with the lack of knowledge regarding the molecular mechanisms that regulate lipid biosynthesis and mobilization in the male gametophyte. In this work, a caleosin gene called OeCLO was cloned from the mature olive (Olea europaea L.) pollen. OeCLO shows sequence homology with the Arabidopsis AtCLO3 and lily LlCLO genes, and encodes a 30 kDa caleosin, which is able to bind calcium in vitro. This caleosin was localized in both the tapetal and germline cells, with its synthesis independently regulated. Expression analyses revealed that caleosin transcripts and the expressed protein appeared at first in the tapetal cells. Thus, the caleosin protein was localized on the surface of large lipid droplets that were densely packed and enclosed by ER cisternae and vesicles, in a similar way to Brassica tapetosomes. After tapetum degeneration, the caleosin-containing remnants were released to the anther loculus and deposited on the surface of the pollen exine, forming the pollen coat. The maturing pollen grain also synthesized the caleosin in the cytoplasm of the vegetative cell, concurrently with the synthesis and accumulation of storage lipids. In the mature pollen, the caleosin was mainly localized attached to the OB boundary, with the N- and C-terminal domains facing the cytoplasm and the hydrophobic central domain embedded in the TAG matrix. During pollen germination, OeCLO transcripts were not detected, while OeCLO protein levels progressively decreased, coincidentally with a decline in the number of OBs [2]. Within the growing pollen tube, the caleosin was mainly located on the OB boundary, the plasma membrane and the vacuole tonoplast. These data strongly suggest that the olive pollen caleosin might function in OB biogenesis and mobilization, as well as in membrane rearrangement phenomena during olive pollen development., Work funded by MICINN and Junta de Andalucía (ERDF-cofinanced projects AGL2008-00517/AGR and P06-AGR-01791). KZ and AZ thank the CSIC for providing JAE-DOC fellowships.

Resumen del póster presentado al 6th European Symposium on Plant Lipids, celebrado del 7 al 10 de julio de 2013 en Bordeaux (France)., In many plants, pollen grains accumulate storage lipids that serve as energy supply during germination. In this work, we have characterized three enzymes (lipase, phospholipase A and lipoxygenase) involved in the early stages of oil body mobilization during olive pollen germination in vitro. In-gel assays showed the presence up to 12 putative lipases in germinated pollen. A single lipolytic band was also associated with oil bodies in the mature and germinated pollen. Early steps of pollen germination were also accompanied by lipoxygenase (LOX) activity. A protein of about 99 kDa with LOX activity was also detected in oil bodies isolated from germinated pollen. Moreover, oil bodies showed phospholipase A, lipase and LOX activities on their surface. Pollen germination was significantly hampered when specific inhibitors of the three enzymes were added to the germination medium or the stigmatic surface. All together, these data support the idea that these enzymes are involved in first steps of oil bodies' breakdown during pollen germination and that this process is essential for proper pollen performance., This work was funded by MICINN (ERDF-cofinanced projects AGL2008-00517 and BFU2011-22779) and Junta de Andalucía (ERDF-cofinanced project P1 0-CVI-5767, P10-AGR-6274 and P11-CVI-7487). KZ and AZ thank the CSIC for providing JAEDOC grants.

Resumen del póster presentado al World Allergy and Asthma Congress, celebrado en Milan (Italia) del 22 al 26 de junio de 2013., [Background]: Olive pollen (Olea europaea -OE-) is a major cause of respiratory allergy among Mediterranean countries and elsewhere in the world. Olive germplasm is exceptionally wide, with more than 250 cultivars only in Spain. Important allergenic differences have been recognised among pollen from different cultivars, involving most relevant allergens like Ole e 1. These differences may influence the clinical performance of OE extracts depending on the sensitivity of individual patients to specific allergens, hence cultivar origin should be taken into account in the manufacture of allergen extracts. As handling and characterisation of a large number of cultivar extracts is impracticable under industrial and clinical standards, the present work intends to define a limited number of cultivar models, characterised by distinctive pollen allergen profiles., [Method]: The Ole e 1, Ole e 2, Ole e 5 and Ole e 9 content was analyzed using specific antibodies in OE extracts prepared from pollen of ten olive cultivars: ′Picual′, ′Manzanilla′, ′Arbequina′, ′Blanqueta′, ′Cornicabra′, ′Verdial′, ′Lechın′, ′Hojiblanca′, ′Lucio′ and ′Loaime′. Allergen semi-quantization was performed by SDS-PAGE and immunoblotting densitometry (Quantity One, Bio-Rad Laboratories), normalizing data for each allergen against the cultivar with the higher concentration. Threshold percentages were defined in order to characterise individual levels of an allergen for each cultivar as low, average and high., Levels of Ole e 2 and Ole e 5 were not informative. Based on Ole e 1 and Ole e 9 expression cultivars could be grouped in three categories: High Ole e 1; high Ole e 9: ′Hojiblanca′; ′Loaime′ High Ole e 1; low Ole e 9: ′Picual′; ′Manzanilla′, ′Lucio′, ′Cornicabra′, ′Lechın′, ′Blanqueta′ Low Ole e 1; low Ole e 9: ′Arbequina′; ′Verdial′, [Conclusion]: In this study we have defined a strategy for grouping of OE cultivars on the basis of differences in relevant allergen expression. This could add specificity to the use of OE extracts in diagnosis and therapy. This preliminary proposal should be implemented by further analyzing additional olive pollen allergens and other relevant cultivars, and testing for the clinical correlates of these allergenic differences.

Resumen del póster presentado al 3rd Joint Congress of the Portuguese and Spanish Microscopy Societies and Israel Society for Microscopy as Invited Guest: Microscopy at the Frontiers of Science, celebrado en Tarragona (España) del 17 al 20 de septiembre de 2013., This work was supported by by ERDF-cofunded projects BFU2011-22779, P2010-AGR6274, P2010-CVI5767 and P2011-CVI-7487. EGQ thanks the MINECO for FPI grant funding.

This work has been supported by numerous research projects. Currently active projects include EFDF confunded grants BFU2011-22779 (Ministerio de Economía y Competitividad), P2010-CVI5767, P2010-AGR6274 and P2011-CVI7487 (Junta de Andalucía), and PEOPLE-PIOF-GA-2011-301550 (European Research Council)

Resumen del póster presentado a la 11th International POG Conference: "Reactive Oxygen and Nitrogen Species in Plants", celebrado de 17 al 19 de julio de 2013 en Varsovia (Polonia)., Transcriptome analysis of the olive stigma and pollen grain was carried out at different developmental stages and during in vitro pollen germination, respectively. Isolated mRNA was used to build cDNA libraries, which were subjected to 454+ sequencing. Readings were combined with sequences obtained from subtractive libraries, in order to produce a de novo assembly of the olive reproductive transcriptome. The presence of transcripts corresponding to major enzymes regulating ROS metabolism was assessed. Sequences corresponding to catalases, superoxide dismutase, dehydroascorbate reductase, GSNO reductase, NADPH oxidase, lipoxygenase and thioredoxins were retrieved and analysed using basic descriptive tools. Expression of transcripts in pollen and pistil was analysed by semiquantitative RT-PCR. Cellular localization of several enzymes was also carried out by using immunocytochemistry. We discuss how these enzyme activities may be involved in the olive pollen metabolism through germination, in the olive stigma over the periods before, after and during receptivity, and how they may contribute to the interaction pollen-stigma., Work supported by ERDF cofunded grants BFU2011-22779, AGL2011-24428, P2010-AGR6274, P2011-CVI7487.

Resumen del trabajo presentado al II Plant Genomics Congress, celebrado en Londres (UK) del 12 al 13 de mayo de 2014., Olive transcriptome has been recently built as the result of library screening through the use of different NGS strategies. Such transcriptome mainly relies on vegetative reads. However, the peculiarity of the reproductive tissues, and the widely reported presence of numerous tissue-specific transcripts in both stigma and pollen grains made us to attempt a similar approach in the later tissues. mRNA was isolated from olive stigmas and pollen grains at different developmental stages and during in vitro pollen germination, respectively. cDNA libraries were built from the isolated mRNA, and subjected to 454/Roche Titanium+ sequencing. Moreover, NGS readings were combined with additional sequences obtained from subtractive libraries, in order to produce a de novo assembly of the olive reproductive transcriptome. Several public software and other tools developed by authors were used for this purpose. Sequences were assembled together into 58 098 (stigma) and 26 422 (pollen) contigs, respectively. Functional annotation is currently underway, however numerous gene products have been previously analysed, particularly those corresponding to major enzymes regulating ROS metabolism. Some of them, including catalases, superoxide dismutase, dehydroascorbate reductase, GSNO reductase, NADPH oxidase, lipoxygenase and thioredoxins, have been further analysed using basic descriptive tools., Work supported by ERDF cofunded grants BFU2011-22779, AGL2011-24428, P2010-AGR6274, P2011-CVI7487

Trabajo presentado al Microscopy at the Frontiers of Science Congress, celebrado en Tarragona (España) del 17 al 20 de septiembre de2013., This work was supported by by ERDF-cofunded projects BFU2011-22779, P2010-AGR6274, P2010-CVI5767 and P2011-CVI-7487, and a research agreement CSIC (Spain)-AVCR (Czech Republic) Ref. 2010CZ0001. MJ Jiménez-Quesada thanks the CSIC for JAE grant funding.

Resumen del póster presentado al World Allergy and Asthma Congress (EAACI-WAO), celebrado del 22 al 26 de junio de 2013 en Milan (Italia)., [Background] Olive pollen (Olea europaea-OE-) is a major cause of respiratoryallergy among Mediterranean countriesand elsewhere in the world. Olive germ-plasm is exceptionally wide, with morethan 250 cultivars only in Spain. Important allergenic differences have been recognised among pollen from different cultivars, involving most relevant allergens like Ole e 1. These differences may influence the clinical performance of OE extracts depending on the sensitivity of individual patients to specific allergens, hence cultivar origin should be taken into account in the manufacture of allergen extracts. As handling and characterisation of a large number of cultivar extracts is impracticable under industrial and clinical standards, the present work intend to define a limited number of cultivar models, characterised by distinctive pollen allergen profiles., [Method] The Ole e 1, Ole e 2, Ole e 5 and Ole e 9 content was analyzed using specific antibodies in OE extracts prepared from pollen of ten olive cultivars: ′Picual′, ′Manzanilla′, ′Arbequina′, ′Blanqueta′, ′Cornicabra′,′Verdial′, ′Lechín′, ′Hojiblanca′, ′Lucio′ and ′Loaime′. Allergen semi-quantization was performed by SDS-PAGE and immunoblotting densitometry (Quantity One, Bio-Rad Laboratories), normalizing data for each allergen against the cultivar with the higher concentration. Threshold percentages were defined in order to characterise individual levels of an allergen foreach cultivar as low, average and high., [Results] Levels of Ole e 2 and Ole e 5 were not informative. Based on Ole e 1 and Ole e 9 expression cultivars could be grouped in three categories: High Ole e 1; high Ole e 9: ′Hojiblanca′; ′Loaime′ High Ole e 1; low Ole e 9: ′Picual′; ′Manzanilla′, ′Lucio′, ′Cornicabra′,′Lechín′, ′Blanqueta' Low Ole e 1; low Ole e 9: ′Arbequina′; ′Verdial'., [Conclusion] In this study we have defined a strategy for grouping of OE cultivarson the basis of differences in relevant allergen expression. This could add specificity to the use of OE extracts in diagnosis and therapy. This preliminary proposal should be implemented by further analyzing additional olive pollen allergens and other relevant cultivars, and testing for the clinical correlates of these allergenic differences.

Trabajo presentado a la XVI edición de la Feria Internacional del Aceite de Oliva e Industrias Afines (Expoliva), celebrada en Jaén del 8 al 11 de mayo de 2013., La industria alimentaria produce rutinariamente aislados proteicos a partir de harinas desengrasadas de diversas fuentes vegetales. Estos aislados proteicos se utilizan en la elaboración de productos alimenticios de calidad y con propiedades funcionales mejoradas respecto a las harinas iniciales. Las fuentes proteicas más utilizadas son la soja, cacahuete, trigo y diversas leguminosas. El olivo posee semillas que no son hasta el momento utilizadas por estas industrias de forma rutinaria, a pesar de su alto contenido proteico. El presente trabajo explora diversos métodos para la preparación de aislados proteicos a partir de esta fuente, y describe su posterior caracterización bioquímica mediante electroforesis en geles de poliacrilamida (SDS-PAGE) y su contenido en proteínas de tipo 11S similares a leguminas. Finalmente, se describe la localización celular y subcelular de los lugares de acumulación de estos componentes. Los resultados obtenidos son de gran utilidad para establecer el potencial de esta fuente alimentaria y servirán de base a posteriores análisis nutricionales en distintos animales de experimentación.

Proceedings of the I Congress PIIISA celebrado en la Estación Experimental del Zaidín, Granada, el 26 de abril de 2012., Up to date, early flower development and the fundamental changes accompanying preand post‐fertilization in Cucurbitaceae (watermelon, melon, zucchini, cucumber….) have not been sufficiently described at the morphological level. Some varieties of Cucurbitaceae recently emerged as commercial trade, are generating a great success because of their high quality (they are very sweet), and their great agronomic value (large size, resistance to diseases, high yield, harvesting time...). Production of these fruits in the greenhouse requires the plants to be pollinated effectively. Bees and bumblebees are introduced artificially in greenhouses and usually carry out this duty. However, the efficiency of the process is not very high at present and farmers need it to be improved. This requires detailed knowledge of how pollen production takes place, how and when the female flowers reach their receptivity, and whether pollen is viable and can or cannot germinate as well as many other details of fertilization. The main aim of this work was to characterize the morphological stages of floral development in two plant models: watermelon (Citrullus lanatus) and zucchini (Cucurbita pepo), by analyzing some structural aspects of their flowers in detail., This work was supported by FEDER funds: The project MEyC BFU2011‐22779 and from “proyectos de excelencia”: (JA) P2010‐AGR6274, P2010‐CVI5767 and P2011‐CVI‐7487.

Trabajo presentado a la II Jornada sobre Investigación Biomédica, celebrada en la Universidad de Jaén el 20 de diciembre de 2012.

Resumen del póster presentado al IV Spanish and Portuguese Meeting on Free Radicals, celebrado en Valencia (España) del 5 al 7 de junio de 2012., Olive pollen is a major cause of allergy in Mediterranean countries. Numerous studies are devoted to the characterization of allergy-eliciting proteins from this allergenic source. However, recent research is focusing on the study of other non-allergenic protein and non-protein components modulating the allergic immune response. These molecules include pollen-associated lipid mediators, adenosine, proteases and oxidases, particularly NAD(P)H oxidase (NOX) among others. The production of superoxide by NOX activity in various allergenic pollens has been recently described. NOX activity has been shown to induce oxidative stress in cultured airway epithelium, driving to allergic inflammation. We report here the presence, molecular characteristics and expression of a NOX enzyme in olive pollen. Moreover, we describe the presence, activity and cellular localization of a well characterized allergenic protein from olive pollen (Ole e 5) displaying relevant homology with Cu/Zn SODs from different species, and a Mn-SOD also from olive pollen. The incidence of Ole e 5 in type I hypersensitivity reactions to olive pollen has been reported to be 35%, whereas several Mn-SODs have also been identified as allergens in different species. In addition to their intrinsic allergenic properties, both enzymes may regulate the levels of NOX-generated superoxide, therefore modulating the inflammation response to this pollen. A model of the interactions occurring among these molecules themselves, their substrates/products and the immune system is proposed., This work was supported by by ERDF-cofunded projects BFU2011-22779, P2010-AGR6274, P2010-CVI5767 and P2011-CVI-7487, Czech Science Foundation grant GACR 522/09/P299 to M.P. and a research agreement CSIC (Spain)-AVCR (Czech Republic) Ref. 2010CZ0001. MJ Jiménez-Quesada and A Zafra thanks the CSIC for JAE grant funding.

Resumen del póster presentado al 1er Congress of the Spanish Network of Advanced Optical Microscopy, celebrado en Barcelona en 2012., The success of fertilization in flowering plants largely depends on the ability of the palien grain to form a palien tube, which must be able to penetrate the stigma surface and move down the style towards the embryo sac. The palien tube is a cylinder-shaped extrusion of the vegetative cell cytoplasm that, unlike most plant cells, elongates strictly at its apex. Consequently, palien tubes exhibit a markedly asymmetric functional behavior in many cellular processes including ion fluxes, cell wall assembly and cytoskeletal arrangements. Palien for most of plants is easy to germinate in vitro, so it can be manipulated under controlled conditions using a wide range of technologies. In many plant species, the palien grain accumulates a high number of organelles at maturity. Sorne of them like oil bodies serve as carbon supply during the palien tube growth. Therefore, palien tubes constitute a good model to study storage lipid mobilization. Thus, confocal live-cell imaging in combination with vital stains allows studying organelles distribution, motility and behavior within palien tubes. The use of fluorescent probes in combination with live imaging techniques is also a powerful tool to study those enzymatic activities directly involving organelles metabolism, such as lipases and phospholipases in the case of oil bodies. Functional analysis of these organelle-associated proteins can be also carried out combining live-cell imaging and microinjection techniques using molecules like antibodies, inhibitors, etc., Work supported by ERDF co-funded projects AGL2008-00517AGR, PE2010-CVI5767 and BFU2011-22779.

Resumen del trabajo presentado a las III Jornadas de Jóvenes Investigadores en Proteómica, celebradas en Santiago de Compostela, Febrero 2012., Este trabajo ha sido cofinanciado con fondos FEDER a través de los proyectos AGL2008-00517/AGR del MICINN y CVI-5767 de la Junta de Andalucía.

Resumen del póster presentado al 20th International Symposium on Plant Lipids, celebrado en Sevilla (España) del 8 al 13 de julio de 2012., The olive (Olea europaea L.) tree is an oil-storing plant of great agronomical importance in the Mediterranean basin. The anther tapetal cells contains several lipidic structures, such as pro-orbicules and plastoglobuli, while mature pollen grains store neutral lipids in the form of sphere-shaped structures called oil bodies. However, little is known about the molecular mechanisms involved in the biogenesis and mobilization of these organelles. Caleosins are oil body-associated proteins involved in storage lipid mobilization during seed germination. In this work, a 30 kDa caleosin, which is able to bind calcium in vitro, was identified in both the tapetal and germ line cells, with its synthesis independently regulated. Thus, the protein was firstly localized in the tapetum, the exine of developing microspores and the surrounding locular fluid. As anthers developed, tapetal cells showed the presence of structures constituted by caleosin-containing lipid droplets closely packed and enclosed by ER-derived cisternae and vesicles. After its degradation, the caleosincontaining remnants of the tapetum were released to the anther locule and filled the cavities of the mature pollen exine, forming the pollen coat. During pollen maturation, the caleosin also accumulated in the vegetative cell, concurrently with the number of oil bodies. Within the pollen grain, the protein was mainly attached to the boundaries of oil bodies, displaying a structural conformation similar to seed oil body-associated caleosins. During pollen germination, the protein level progressively decreased, coincidentally with a decline in the number of OBs. Within the growing pollen tube, the protein was mainly localized on the surface of OBs, but a pool of caleosins was also visualized in the cytoplasm at the subapical region. All these findings strongly suggest that this caleosin might have a function in oil body biogenesis and mobilization during anther development., This work was supported by ERDF co-financed projects AGL2008-00517/AGR (MICINN) and P06-AGR-01791 (Junta de Andalucía).

Resumen del póster presentado al IV Spanish and Portuguese Meeting on Free Radicals, celebrado en Valencia del 5 al 7 de junio de 2012., The presence of high levels of NO has been described in several pollen grains, where their presence has been shown to regulate pollen tube growth and orientation. Here we have assessed the production of NO during in vitro germination in the olive pollen by using exogenous NO donors like sodium nitroprusside and S-nitrosoglutathione. Both chemical induced a remarkable inhibition of pollen germination and tube growth, which were partially reverted by the addition of the specific NO-scavenger carboxy-PTIO (cPTIO). The presence and localization of NO was determined either by using a fluorimetric assay and by confocal microscopy using the fluorescent probe diaminoflurescein-2 diacetate (DAF-2DA). As olive pollen is also a highly allergenic source, the putative role of NO in eliciting the hypersensitive response in humans is discussed., This work was supported by by ERDF-cofunded projects BFU2011-22779, P2010-AGR6274, P2010-CVI5767 and 2011-CVI-7487. MJ Jiménez-Quesada and A Zafra thanks the CSIC for JAE grant funding.

Foods from plant origin, particularly nuts and seeds, represent the major source of registered food allergy due to the high variability of plant allergenic molecules, which depends on plant growing conditions, seed/fruit ripening, environmental stresses and/or industrial processing. Maize, one of the major human diet components, is one of the most world widely consumed cereals. It has become therefore one of the major causes of food allergy due to the widespread corn derived food products, which make difficult itsto avoid it. However, detailled biochemical knowledge of maize allergy is lacking. There are several unanswered questions including the symptoms and mechanisms involved in maize allergenic reactions, its prevalence in adults and children, the implicated allergen molecules and the clinical cross-sensitization. Therefore, diagnostic tests and maize allergy management constitute a field of great interest. Currently, maize allergen proteins are classified into 20 different families, displaying diverse structures and functions. They are responsible for many IgE cross-reactions between unrelated pollen and plant food allergen sources. The most relevant maize allergen molecules belong to the expansin and the Ole e 1 superfamilies, the panallergen profilin, and the Lipid Transfer Proteins (LTPs), i.e. Zea m 14, the major maize allergen. Agricultural biotechnology promises plant food production through genetically modified (GM) crops with improved agronomic characteristics and enhanced consumer benefits. Numerous efforts in plant breeding programs have resulted in producing low-allergen or allergen-null plants that could moderate the allergic response. For this purpose, identification of new genes, and knowledge of allergen protein structures and function is crucial for the understanding of biochemical processes inducing maize allergy. Computational biology and protein modeling are increasingly used to evaluate whether a novel protein correspons to a known allergen, has a potential to become an allergen or could possibly cross-react with another existing allergen. In order to answer the above crucial questions and bring insights into the processes of food allergy, we focus our attention in this review on maize allergens protein families, their biochemical, structural and immunological properties, while discussing possible strategies to predict biological consequences of allergen sensitization and cross-reactivity as well as therapies to mitigate maize allergy., This research has been partially funded by ERDF-cofinanced projects BFU2011-22779 (MCINN), P2010-AGR6274 and P2011-CVI-7487 (Andalusian Regional Government).

Resumen del trabajo presentado al 20th International Symposium on Plant Lipids (ISPL), celebrado en Sevilla del 8 al 13 de julio de 2012.

Resumen del trabajo presentado a las IV Jornadas Internacionales de la Aceituna de Mesa, celebradas en España en febrero de 2012., [ES] La industria de elaboración de aceituna de mesa genera importantes cantidades de hueso de aceituna. Las semillas del olivo contienen grandes cantidades proteínas y lipidos en los tejidos de reserva que las integran, especialmente en el cctiledón y el endospermo. Uno de estos componentes proteicos mayoritarios de las semillas son las proteínas de almacenamiento de semillas 11S de tipo leguminas. así como oleosinas, legumainas, caleosinas y enzimas de las distintas rutas biosintéticas y degradativas. Actualmente estamos realizando estudios destinados a caracterizar en detalle la organización tisular de la semilla del olivo, así como la composición proteica y lipídica de sus distintos tejidos tanto en la aceituna madura como durante el desarrollo del fruto, y en el fruto maduro de distintos cultivares. Esta linea de trabajo incluye estudios aplicados para estimar el uso potencial de estos componentes como valor añadido del hueso, por ejemplo para su uso en alimentación animal., [EN] The industry of table olive elaboration generales relevan! amounts of olive stones. Olive seeds contain high protein and lipid contents in their tissues, especially in the cotyledons and the endosperm. One of the majar protein components of !he olive seeds corresponds to 11 S storage proteins of "legumin" type. as well as oleosins, legumains, caleosins and enzymes involved in different biosynthetic and degradation pathways. We are currently investigating in detall the tissue organization of the olive seeds as well as the protein and lipid components of their difierent tissues both at maturity and during fruit development, and in the mature seeds of difierent cultivars. This research includes applied studies to estímate the potential use of these components as an additional value for the stones, for example in animal nutrition., Investigación cofinanciada con fondos FEDER (proyectos BFU2011-22779, P2010-AGR6274 y P2011-CVI-7487).

Muñoz Dorado, José et al.-- Scientific editors: Alché Ramírez, Juan de Dios; Aranda, Elisabeth; Campos, Mercedes; Corpas, Francisco J.; García Romera, Inmaculada; Jiménez Zurdo, José I.; Martínez-Abarca, Francisco; Palma Martínez, José Manuel; Paredes, Daniel; Sampedro, Inmaculada.-- 46 páginas, con gráficos e imágenes.-- Proceedings of the I Congress PIIISA celebrado en la Estación Experimental del Zaidín, Granada, el 26 de abril de 2012., [ES] Lo que hay escrito a lo largo de estas páginas es el fruto de una andadura que comenzó en Noviembre de 2011: el proyecto PIIISA2012, al que algunos medios de comunicación calificaron como “Investigación con Acné”. El nombre era de lo más sugerente y apropiado, pero había que darle forma y resultado. Han sido casi seis meses de esfuerzo con mayúsculas. Esfuerzo sobre todo por parte de los jóvenes (alumnos de secundaria y Bachillerato), pero también de los investigadores a su cargo, así como de los profesores coordinadores de secundaria. Cada Proyecto ha tenido sus propias peculiaridades y dificultades, y como todo proyecto de Investigación, sus éxitos y sus fracasos; pero todos ellos han tenido un denominador común: la ilusión por conocer la ciencia y todos sus aspectos más escondidos. Los estudiantes han experimentado con la decepción, el acierto, la satisfacción final del trabajo acabado, la publicación y, lo más importante, la presentación a la sociedad que nos ha financiado y nos permite este lujo: el de INVESTIGAR. Estos alumnos “privilegiados” han entrado en contacto con la CIENCIA en mayúsculas desarrollando experimentos nunca realizados por otros. A su vez los investigadores han tenido que ser lo suficientemente flexibles y dinámicos para combinar el objetivo de estos proyectos (ilusionar a adolescentes por la ciencia) con la realidad y prioridades de unos alumnos de secundaria y bachillerato (el acné). A este último aspecto los coordinadores del proyecto estaban mucho más acostumbrados. El resultado lo podréis apreciar y disfrutar a lo largo de estas páginas, que seguro os sorprenderán. Que este libro sea no sólo un reflejo de lo que han sido estos seis meses sino el germen de un apasionante futuro., [EN] What is written along these pages is the fruit of a long journey that began back in November 2011: project PIIISA2012, which some media have described as “Research with Acne”. The name was indeed very appropriate, but it was necessary to actually put it into shape. We spent almost six months to do that devoting much of our time and efforts, as did the teen students involved in the project and their teachers and persons in charge. Each Project has had its own peculiarities and difficulties, and as in every research project, its successes and its failures. Nonetheless, each and every one of them had a common denominator: the excitement of science itself and of all its more hidden aspects. The students have experienced disappointment, success and the final satisfaction of the work well done, as well as its publication and dissemination to the general public responsible of our financing and of making research real and tangible. These “privileged” students have come into contact with the SCIENCE, developing experiments that had never been done before. Researchers, on their side, had to be the sufficiently flexible and dynamic to combine the aim of these projects (making science exciting and attractive to teenage students) with their everyday life and top priorities (acne). Needless to say that the coordinators of the projects were far more familiarized with this unsightly problem than with the more scientific aspects. The results of this endeavour will be thoroughly enjoyed along these pages and will surely surprise you. We hope that this book will not only be a reflection of what these past six months have been, but that it will also set the basis for an exciting scientific future., Biotic interactions in soil and rhizosphere: predation of Sinorhizobium meliloti by Myxococcus xanthus: this work was performed in the Microbiology and Symbiotic Systems Department at Estación Experimental del Zaidín (Consejo Superior de Investigaciones Científicas) within the frame of the Young Students in Science Program (PIIISA2012; http://emc2astronomy.blogspot.com/).It was mainly supported by research project MICINN Consolider‐Ingenio 2010 CSD2009‐00006 and Institutos de Educación Secundaria de Granada: Zaidín Vergeles, Angel Ganivet, Francisco Ayala, Mariana Pineda and Padre Manjón. We also appreciate the coordination of teachers Antonio Quesada, Francisco Javier López, Mª Concepción Martínez and Javier Cáceres.-- Does garlic‐generated nitric oxide (NO) affect the physiology of pepper fruits?: this work was supported by the ERDF‐cofinanced grant AGL2008‐00834 from the Ministry of Science and Innovation, Spain.-- Dry olive residue bioremediation by new saprobe fungi: this work was supported by the Ministerio de Economia y Competitividad (AGL2008‐00572).-- Flower development and pollen morphology in Cucurbitaceae: this work was supported by FEDER funds: The project MEyC BFU2011‐22779 and from “proyectos de excelencia”: (JA) P2010‐AGR6274, P2010‐CVI5767 and P2011‐CVI‐7487.-- Has cupper ion any effect on Sinorhizobium meliloti bacteria?: it was supported mainly by research project MICINN Consolider‐Ingenio 2010. CSD2009‐00006; Scientific research by Young Students in Science (PIIISA2012; http://emc2astronomy.blogspot.com/). Institutos de Educación Secundaria de Granada: Padre Manjón, Angel Ganivet y Fray Luis de Granada., Contiene: Biotic interactions in soil and rhizosphere: predation of Sinorhizobium meliloti by Myxococcus xanthus.Gabriela Tarifa Álvarez; Irene Marín Sánchez; Clara Pérez González; Ana Villar del Paso; Mª Ángeles Yélamos Lorente; Nazaret Ceballos Miján; Raúl Peña López; Manuel Jurado de Haro; José Muñoz Dorado; Juana Pérez; José I. Jiménez Zurdo. -- Determination of individualized breeding methods of Anthocoris nemoralis neonate nymphs. Nuria Sainz; Begoña Castillo; Carolina García; Francisco Sánchez; Ángela Lirola; Elio Gugliere; Mercedes Campos; Iván Batuecas; Daniel Paredes.-- Does garlic‐generated nitric oxide (NO) affect the physiology of pepper fruits?. Nuria García Carbonero; Carlos Nombela Durán; Inmaculada Caballero Guzmán; María del Mar Quesada Díaz; Pilar Montoza García; Alejandra Sánchez Alonso; María Jesús Campos; Carmelo Ruiz; Francisco J. Corpas; José Manuel Palma.-- Dry olive residue bioremediation by new saprobe fungi. María Naranjo Márquez; Ignacio Romero García; Belén Sola Bullejos; José Manuel Ruiz González; Álvaro Arregui Morales; Pablo Ortega Martín; Tomás Ortega Martín; Ramón de la Chica Mora; Carmen Fernández Lacal; David Jiménez Carretero; Elisabet Aranda; Inmaculada Sampedro; Inmaculada García‐Romera.-- Flower development and pollen morphology in Cucurbitaceae. David G. Caracuel; Antonio Jesús Castro; Ana María Cogolludo; Carlos Enríquez; José Carlos Morales; Irene Ruiz‐Gámez; María Victoria Ruiz‐Maldonado; Sergio Torreblanca; Guillermo Vicente; Agnieszka Zienkiewicz; Krzysztof Zienkiewicz; Juan de Dios Alché.-- Has cupper ion any effect on Sinorhizobium meliloti bacteria?. Blanca Barón Torcal; Ana Guardia Molina; Ángela Liébana Medina; Sara Miñán Polo; José Manuel Sanjuán Parra; Francisco Martínez‐Abarca.

Resumen del trabajo presentado a las I Jornadas sobre Investigación Biomédica: Máster Universitario en Biotecnología y Biomedicina, celebradas el 20 de enero de 2012 en la Universidad de Jaén.

Trabajo presentado a las III Jornadas Nacionales del grupo de Horticultura de la SECH, celebradas en Sevilla el 6 y 7 de octubre de 2011.

Resumen de la comunicación presentada al XV Simposio Científico-Técnico del aceite de oliva (Expoliva 2011) celebrado en Jaén (España) del 11 al 13 de Mayo de 2011.

pp:607-613 Publisher: Formatex Research Center, Several components of the exine (i.e. the outer layer of the pollen wall) show conspicuous autofluorescence in the pollen grain. We describe the autofluorescence properties of pollens from the Mediterranean cypress, lily, turnip and olive (cvs. Picual, Loaime, Cornicabra and Ayrouni). The intensity and pattern of autofluorescence largely varied depending on the species and the hydration stage of pollen. In the case of olive, differences in the level of autofluorescence were detected among the four cultivars analyzed. This technique also allowed further morphological characterization of pollen (e.g. determination of pollen size and shape, and the number and type of apertures, the pattern of the exine, etc), evaluation of pollen integrity, detection of additional components (e.g. the pollen coat) over the surface of the pollen wall, etc. The methods used can be considered non-disruptive and lack of sample preparation. Monitoring pollen autofluorescence could be useful in disciplines like Aerobiology, Plant Taxonomy, and Cell and Molecular Biology.

111 páginas, 6 tablas, 4 figuras.-- Numero y fecha de presentación de la solicitud: P200500047 del 12 de Enero del 2005. Titular: Consejo Superior de Investigaciones Científicas (CSIC)., Se han aislado, caracterizado e identificado ácidos nucleicos que codifican péptidos, o fragmentos de los mismos, que comprenden, al menos, .un epítopo del alérgeno Ole e 1 de polen de olivo de variedades definidas de olivo (Olea europea L.). Dichos péptidos, que poseen uno o más epítopos alergénicos del alérgeno mayoritario del polen de olivo (Ole e 1) tienen actividad alergénica y pueden ser utilizados, entre otras aplicaciones, en el diagnóstico y tratamiento de la alergia al polen de olivo. Dicha actividad alergénica de dichos péptidos o fragmentos de los mismos puede ser modulada mediante la introducción de modificaciones apropiadas con el fin de obtener variantes hiopoalergénicas o hiperalergénicas. El empleo de alérgenos recombinantes específicos de variedades definidas de olivo permite, además, determinar perfiles individualizados de reactividad para cada paciente y el diseño de inmunoterapias personalizadas.

Comunicación presentada al XVII International Symposium APLE celebrado en Ourense (España) del 7 al 10 de Julio de 2010., This work was supported by Spanish Ministry of Science and Innovation (MCel, project AGL2008-00517/AGR) and the Junta de Andalucía (project P06-AGR-01791).

1 página.-- Resumen del poster presentado en el XXI International Congress on Sexual Plant Reproduction, celebrado del 2 al 6 de Agosto de 2010 en Bristol.-- PSR5.19., This work was supported by MICINN (ERDF co-financed project AGL2008-00517/AGR) and Junta de Andalucía (project P06-AGR-01791).

1 página.-- Resumen de la comunicación presentada al 28º International Horticultural Congress celbrado del 22 al 27 de 2010 en Lisboa.-- S08.244 Olivetrends.

1 página.-- Resumen de la comunicación presentada al 28º International Horticultural Congress celebrado del 22 al 27 de 2010 en Lisboa.-- S08.213 Olivetrends.

Trabajo presentado al XVIII Congress of the Federation of European Societies of Plant Biology (FESPB), celebrado en Valencia (España) del 4 al 9 de julio de 2010.

Resumen del póster presentado al 28th International Horticultural Congress, celebrado en Lisboa (Portugal) del 22 al 27 de agosto de 2010., Ole e1 is a member of the “pollen proteins of the Ole e1 family” which produces IgE-reactivity in >70% of patients allergic to olive pollen. In order to precisely determine the nature of the differences in the protein expression pattern of Ole e1 for cultivars of different characteristics, cvs. ‘Rowghani’ and ‘Zard’, crude protein was extracts and Isoelectric focusing (IEF) was performed in a IPGphor device (Pharmacia) and analytical gel electrophoresis was performed according to Laemmli as well. Gels were transferred onto PVDF membrane and then probed with a monoclonal antibody to Ole e1. An Alexa Fluor 488 anti-mouse IgG antibody made in goat (Molecular Probes) was used at as the secondary antibody. Approximately 750 spots could be detected by silver staining in the 2-D map of each sample. Up to 9 of these spots were immunoreactive to the Ole e1 antibody after Western blotting experiments in the cultivar Rowghani, whereas up to five immunoreactive spots only were detected in the cultivar Zard. The high degree of polymorphism displayed by Ole e1 in a cultivar-specific manner may represent part of a unique recognition system. Moreover, it’s probably relation to different biological behaviours of corresponding cultivars are discussed.

[ES] La calidad del polen es un parámetro de gran utilidad en estudio agro-biológico y que incluye tanto la viabilidad como la capacidad de germinación del grano de polen. En el caso del polen del olivo (Olea europaea L.) tiene especial inrerés para seleccionar cultivares polinizadores. En este trabajo se han analizado los siguientes métodos para evaluar la calidad del polen: a) unción con azul tripán, b) reacción fluorocromática con diacetato de fluoreceína y e) capacidad de germinación in v itro del polen. Los resultados obtenidos indican que los dos último son lo métodos más efectivos para la estimación de la calidad del polen, y han permitido clasificar los cultivares estudiados de acuerdo con su carácter de polinizador potencial., [EN] Pollen quality is a useful parameter for agrobiological studies, which includes both the viability and rhe capacity of the pollen grain 10 germinate. In the case of the olive (Olea europaea L.) pollen, such parameter is of particular interest for the seleclion of pollen donar cultivar. In this work we have compared several method to evaluate the quality of pollen, including: a) trypan blue staining, b) the fluorochromatic reaction of the fluorescein diacetate, and e) the germtnation ability of pollen in vitro. The results obtained indicate that the two latter methods were the most effective to estimate olive pollen quality, and allowed us to classify the cultivars studied according to their pollinating potential., Este trabajo ha sido financiado por los proyectos AGL2008-00517/AGR y BFU 2008-00629 (Ministerio de Ciencia e Innovación) y por la Consejería de Ciencia y Tecnología de la Junta de Andalucía (Grupo de Investigación BI0283). J. D. Rejón agradece al Ministerio de Ciencia e Innovación la concesión de una beca/contrato FPU.

Resumen del trabajo presentado al "Biochemical Society Focused Meeting" celebrado en Bath (Reino Unido) del 14 al 16 de Septiembre de 2009., Work supported by projects P06-AGR-01719 and BFU2008-00629.

2 páginas, 1 figura.-- Trabajo presentado al "XXIV Congress of the Spanish Microscopy Society" y al "XLIV Annual Meeting of the Portuguese Society for Microscopy" celebrados en Segovia (España) del 16 al 19 de Julio de 2009., This work was supported by research projects P06-AGR-01719 (Andalusian Regional Government) and BFU2008-00629 (Spanish MCyT).

Resumen del trabajo presentado a la XVIII Reunión de la Sociedad Española de Fisiología Vegetal (SEFV) y al XI Congreso Hispano-Luso de Fisiología Vegetal, S2-P24: Crecimiento y desarrollo, celebrados en Zaragoza (España) del 8 al 11 de Septiembre de 2009., This work was supported by research projects BFU2008-00629 (Spanish MCyT) and P06-AGR-01719 (Andalusian Regional Government). A Zafra thanks the CSIC for providing JAE grant funding.

Resumen del trabajo presentado al Olivebioteq, S2-P10, celebrado en Sfax (Túnez) del 15 al 19 de Diciembre de 2009.

Resumen del trabajo presentado a la XVIII Reunión de la Sociedad Española de Fisiología Vegetal (SEFV) y al XI Congreso Hispano-Luso de Fisiología Vegetal, celebrados en Zaragoza del 8 al 11 de septiembre de 2009., This work was supported by the Andalusian Regional Government (project P06-AGR-01791). C. Suarez thanks the MEC for providing FPI grant funding.