141 results on '"Bonner E"'
Search Results
52. Effects of glucocorticoid steroids on renal and systemic acid-base metabolism
- Author
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Hulter, H. N., primary, Licht, J. H., additional, Bonner, E. L., additional, Glynn, R. D., additional, and Sebastian, A., additional
- Published
- 1980
- Full Text
- View/download PDF
53. Renal and systemic acid-base effects of chronic hypoparathyroidism in dogs
- Author
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Hulter, H. N., primary, Toto, R. D., additional, Bonner, E. L., additional, Ilnicki, L. P., additional, and Sebastian, A., additional
- Published
- 1981
- Full Text
- View/download PDF
54. Paper 6: Water Treatment for Modern Boiler Plant in the Paper Industry
- Author
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Bonner, E. J., primary
- Published
- 1966
- Full Text
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55. Performance Requirements for Supersonic Transports
- Author
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Greene, L. P., primary and Bonner, E., additional
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- 1964
- Full Text
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56. Expanding Role of Potential Theory in Supersonic Aircraft Design
- Author
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BONNER, E., primary
- Published
- 1971
- Full Text
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57. The expanding role of potential theory in the design of supersonic aircraft
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BONNER, E., primary
- Published
- 1969
- Full Text
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58. The shame of Armenia.
- Author
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Bonner, E.
- Subjects
- *
SOCIAL history - Abstract
Describes the tragic plight of the people of Armenia. Abandoned by government; Attacked by Azerbaijan; Earthquake victims; Responsibility of the Armenians for their plight.
- Published
- 1990
59. South Carolina Bible Society Convention.
- Author
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GADSDEN, C. P., WIGHTMAN, J. T., ANDERSON, J. MONROE, and BONNER, E. A.
- Published
- 1860
60. For whom the bell tolls.
- Author
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Bonner, E. and Bouis, A.W.
- Subjects
- *
POLITICAL science ,SOVIET Union politics & government - Abstract
Describes proceedings at the recent congress in the Soviet Union. Huge gap between the Communist Party elite and the people; People's need for property and power; Need for the Party to relinquish property; President Mikhail Gorbachev and Boris Yeltsin.
- Published
- 1990
61. CYP24A1 splice variants—Implications for the antitumorigenic actions of 1,25-(OH)2D3 in colorectal cancer
- Author
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Horváth, H.C., Khabir, Z., Nittke, T., Gruber, S., Speer, G., Manhardt, T., Bonner, E., and Kallay, E.
- Subjects
- *
COLON cancer , *ANTINEOPLASTIC agents , *CHOLECALCIFEROL , *CYTOCHROME P-450 , *GENETIC engineering , *CANCER cells , *MITOCHONDRIA - Abstract
Abstract: 25-Hydroxyvitamin D3 24-hydroxylase (CYP24A1), the catabolizing enzyme of the active vitamin D3, is often overexpressed in solid tumors. The unbalanced high levels of CYP24A1 seem to be a determinant of vitamin D resistance in tumors. Splice variants of CYP450 enzymes are common. Existence of CYP24A1 isoforms has been reported recently. We have investigated the presence of CYP24A1 splicing variants (SV) in human colon cancer cell lines and tissue samples. Using a set of primer combination we have screened the entire coding sequence of CYP24A1 and identified three splice variants in colon cancer cell lines. The presence of these SVs in human colon tissue samples showed a correlation with histological type of the tissue and gender of patients. The sequencing of the alternatively spliced fragments showed that two have lost the mitochondrial target domain, while the third lacks the heme-binding domain. All SVs retained their sterol binding domain. Translation of these variants would lead to a dysfunctional enzyme without catalytic activity that still binds its substrates therefore they might compete for substrate with the synthesizing and catabolizing enzymes of vitamin D. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
62. British Society of Gastroenterology Best Practice Guidance: outpatient management of cirrhosis - part 2: decompensated cirrhosis.
- Author
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Mansour D, Masson S, Corless L, Douds AC, Shawcross DL, Johnson J, Leithead JA, Heneghan MA, Rahim MN, Tripathi D, Ross V, Hammond J, Grapes A, Hollywood C, Botterill G, Bonner E, Donnelly M, McPherson S, and West R
- Abstract
There are two distinct phases in the natural history of cirrhosis: compensated disease (corresponding to Child Pugh A and early Child Pugh B disease), where the patient may be largely asymptomatic, progressing with increasing portal hypertension and liver dysfunction to decompensated disease (corresponding to Child Pugh late B-C), characterised by the development of overt clinical signs, including jaundice, hepatic encephalopathy (HE), ascites, renal dysfunction and variceal bleeding. The transition from compensated cirrhosis to decompensated cirrhosis (DC) heralds a watershed in the nature and prognosis of the disease. DC is a systemic disease, characterised by multiorgan/system dysfunction, including haemodynamic and immune dysfunction. In this second part of our three-part series on the outpatient management of cirrhosis, we address outpatient management of DC, including management of varices, ascites, HE, nutrition, liver transplantation and palliative care. We also introduce an outpatient DC care bundle. For recommendations on screening for osteoporosis, hepatocellular carcinoma surveillance and vaccination see part one of the guidance. Part 3 of the guidance focusses on special circumstances encountered in patients with cirrhosis, including surgery, pregnancy, travel, management of bleeding risk for invasive procedures and portal vein thrombosis., Competing Interests: Competing interests: DM has received consultancy fees from Falk Pharma; SMcPherson has received personal fees outside the submitted work from Gilead, Intercept and Novonordisk and Norgine Pharmaceuticals; SMasson has received speakers fees from Dr Falk, Norgine Pharmaceuticals, Sandoz; JAL has received speakers fees from Advanz; DLS has undertaken consultancy for Norgine Pharmaceuticals, EnteroBiotix, Mallinckrodt Pharmaceuticals and ONO Pharma UK and has delivered paid lectures for Norgine Pharmaceuticals Ltd, Falk Pharma and Aska Pharmaceutical., (© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)
- Published
- 2023
- Full Text
- View/download PDF
63. British Society of Gastroenterology Best Practice Guidance: outpatient management of cirrhosis - part 1: compensated cirrhosis.
- Author
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Mansour D, Masson S, Shawcross DL, Douds AC, Bonner E, Corless L, Leithead JA, Hammond J, Heneghan MA, Rahim MN, Tripathi D, West R, Johnson J, Botterill G, Hollywood C, Ross V, Donnelly M, Compston JE, McPherson S, and Grapes A
- Abstract
The prevalence of cirrhosis has risen significantly over recent decades and is predicted to rise further. Widespread use of non-invasive testing means cirrhosis is increasingly diagnosed at an earlier stage. Despite this, there are significant variations in outcomes in patients with cirrhosis across the UK, and patients in areas with higher levels of deprivation are more likely to die from their liver disease. This three-part best practice guidance aims to address outpatient management of cirrhosis, in order to standardise care and to reduce the risk of progression, decompensation and mortality from liver disease. Here, in part one, we focus on outpatient management of compensated cirrhosis, encompassing hepatocellular cancer surveillance, screening for varices and osteoporosis, vaccination and lifestyle measures. We also introduce a compensated cirrhosis care bundle for use in the outpatient setting. Part two concentrates on outpatient management of decompensated disease including management of ascites, encephalopathy, varices, nutrition as well as liver transplantation and palliative care. The third part of the guidance covers special circumstances encountered in managing people with cirrhosis: surgery, pregnancy, travel, managing bleeding risk for invasive procedures and portal vein thrombosis., Competing Interests: Competing interests: DM has received consultancy fees from Falk Pharma. SMc has received personal fees outside the submitted work from Gilead, Intercept and Novonordisk and Norgine Pharmaceuticals. SM has received speakers' fees from Dr Falk, Norgine Pharmaceuticals and Sandoz. JAL has received speakers' fees from Advanz. DLS has undertaken consultancy for Norgine Pharmaceuticals, EnteroBiotix, Mallinckrodt Pharmaceuticals and ONO Pharma UK, and has delivered paid lectures for Norgine Pharmaceuticals, Falk Pharma and Aska Pharmaceutical Co., (© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)
- Published
- 2023
- Full Text
- View/download PDF
64. British Society of Gastroenterology Best Practice Guidance: outpatient management of cirrhosis - part 3: special circumstances.
- Author
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Mansour D, Masson S, Hammond J, Leithead JA, Johnson J, Rahim MN, Douds AC, Corless L, Shawcross DL, Heneghan MA, Tripathi D, McPherson S, Bonner E, Botterill G, West R, Donnelly M, Grapes A, Hollywood C, and Ross V
- Abstract
The prevalence of cirrhosis has risen significantly over recent decades and is predicted to rise further. Widespread use of non-invasive testing means cirrhosis is increasingly diagnosed at an earlier stage. Despite this, there are significant variations in outcomes in patients with cirrhosis across the UK, and patients in areas with higher levels of deprivation are more likely to die from their liver disease. This three-part best practice guidance aims to address outpatient management of cirrhosis, in order to standardise care and to reduce the risk of progression, decompensation and mortality from liver disease. Part 1 addresses outpatient management of compensated cirrhosis: screening for hepatocellular cancer, varices and osteoporosis, vaccination and lifestyle measures. Part 2 concentrates on outpatient management of decompensated disease including management of ascites, encephalopathy, varices, nutrition as well as liver transplantation and palliative care. In this, the third part of the guidance, we focus on special circumstances encountered in managing people with cirrhosis, namely surgery, pregnancy, travel, managing bleeding risk for invasive procedures and portal vein thrombosis., Competing Interests: Competing interests: DM has received consultancy fees from Falk Pharma; SMcPherson has received personal fees outside the submitted work from Gilead, Intercept and Novonordisk and Norgine Pharmaceuticals; SMasson has received speakers fees from Dr Falk, Norgine Pharmaceuticals, Sandoz; JAL has received speakers fees from Advanz; DLS has undertaken consultancy for Norgine Pharmaceuticals, EnteroBiotix, Mallinckrodt Pharmaceuticals and ONO Pharma UK and has delivered paid lectures for Norgine Pharmaceuticals, Falk Pharma and Aska Pharmaceutical., (© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)
- Published
- 2023
- Full Text
- View/download PDF
65. Mechanisms regulating transitory suppressive activity of neutrophils in newborns: PMNs-MDSCs in newborns.
- Author
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Perego M, Fu S, Cao Y, Kossenkov A, Yao M, Bonner E, Alicea-Torres K, Liu W, Jiang Z, Chen Z, Fuchs SY, Zhou J, and Gabrilovich DI
- Subjects
- Mice, Animals, Neutrophils, Lactoferrin metabolism, NF-kappa B metabolism, Cytokines metabolism, Lipoproteins, LDL metabolism, Myeloid-Derived Suppressor Cells
- Abstract
Transitory appearance of immune suppressive polymorphonuclear neutrophils (PMNs) defined as myeloid-derived suppressor cells (PMNs-MDSCs) in newborns is important for their protection from inflammation associated with newly established gut microbiota. Here, we report that inhibition of the type I IFN (IFN1) pathway played a major role in regulation of PMNs-MDSCs-suppressive activity during first weeks of life. Expression of the IFN1 receptor IFNAR1 was markedly lower in PMNs-MDSCs. However, in newborn mice, down-regulation of IFNAR1 was not sufficient to render PMNs immune suppressive. That also required the presence of a positive signal from lactoferrin via its receptor low-density lipoprotein receptor-related protein 2. The latter effect was mediated via NF-κB activation, which was tempered by IFN1 in a manner that involved suppressor of cytokine signaling 3. Thus, we discovered a mechanism of tight regulation of immune suppressive PMNs-MDSCs in newborns, which may be used in the development of therapies of neonatal pathologies., (©2022 Society for Leukocyte Biology.)
- Published
- 2022
- Full Text
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66. Postoperative Pain Relief after Pancreatic Resection: Systematic Review and Meta-Analysis of Analgesic Modalities.
- Author
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Akter N, Ratnayake B, Joh DB, Chan SJ, Bonner E, and Pandanaboyana S
- Subjects
- Analgesia, Patient-Controlled, Analgesics therapeutic use, Humans, Pain, Postoperative drug therapy, Pain, Postoperative etiology, Retrospective Studies, Analgesia, Epidural, Pancreatectomy adverse effects
- Abstract
Background: This systematic review explored the efficacy of different pain relief modalities used in the management of postoperative pain following pancreatoduodenectomy (PD) and distal pancreatectomy (DP) and impact on perioperative outcomes., Methods: MEDLINE (OVID), Embase, Pubmed, Web of Science and CENTRAL databases were searched using PRISMA framework. Primary outcomes included pain on postoperative day 2 and 4 and respiratory morbidity. Secondary outcomes included operation time, bile leak, delayed gastric emptying, postoperative pancreatic fistula, length of stay, and opioid use., Results: Five randomized controlled trials and seven retrospective cohort studies (1313 patients) were included in the systematic review. Studies compared epidural analgesia (EDA) (n = 845), patient controlled analgesia (PCA) (n = 425) and transabdominal wound catheters (TAWC) (n = 43). EDA versus PCA following PD was compared in eight studies (1004 patients) in the quantitative meta-analysis. Pain scores on day 2 (p = 0.19) and 4 (p = 0.18) and respiratory morbidity (p = 0.42) were comparable between EDA and PCA. Operative times, bile leak, delayed gastric emptying, pancreatic fistula, opioid use, and length of stay also were comparable between EDA and PCA. Pain scores and perioperative outcomes were comparable between EDA and PCA following DP and EDA and TAWC following PD., Conclusions: EDA, PCA and TAWC are the most frequently used analgesic modalities in pancreatic surgery. Pain relief and other perioperative outcomes are comparable between them. Further larger randomized controlled trials are warranted to explore the relative merits of each analgesic modality on postoperative outcomes with emphasis on postoperative complications., (© 2021. The Author(s).)
- Published
- 2021
- Full Text
- View/download PDF
67. The chemistry and toxicology of vaping.
- Author
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Bonner E, Chang Y, Christie E, Colvin V, Cunningham B, Elson D, Ghetu C, Huizenga J, Hutton SJ, Kolluri SK, Maggio S, Moran I, Parker B, Rericha Y, Rivera BN, Samon S, Schwichtenberg T, Shankar P, Simonich MT, Wilson LB, and Tanguay RL
- Subjects
- Chemistry, Humans, Toxicology, Vaping adverse effects
- Abstract
Vaping is the process of inhaling and exhaling an aerosol produced by an e-cigarette, vape pen, or personal aerosolizer. When the device contains nicotine, the Food and Drug Administration (FDA) lists the product as an electronic nicotine delivery system or ENDS device. Similar electronic devices can be used to vape cannabis extracts. Over the past decade, the vaping market has increased exponentially, raising health concerns over the number of people exposed and a nationwide outbreak of cases of severe, sometimes fatal, lung dysfunction that arose suddenly in otherwise healthy individuals. In this review, we discuss the various vaping technologies, which are remarkably diverse, and summarize the use prevalence in the U.S. over time by youths and adults. We examine the complex chemistry of vape carrier solvents, flavoring chemicals, and transformation products. We review the health effects from epidemiological and laboratory studies and, finally, discuss the proposed mechanisms underlying some of these health effects. We conclude that since much of the research in this area is recent and vaping technologies are dynamic, our understanding of the health effects is insufficient. With the rapid growth of ENDS use, consumers and regulatory bodies need a better understanding of constituent-dependent toxicity to guide product use and regulatory decisions., Competing Interests: Declaration of Competing Interest The authors declare that there are no conflicts of interest., (Copyright © 2021 Elsevier Inc. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
68. Systemic Administration of PTH Supports Vascularization in Segmental Bone Defects Filled with Ceramic-Based Bone Graft Substitute.
- Author
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Freischmidt H, Armbruster J, Bonner E, Guehring T, Nurjadi D, Bechberger M, Sonntag R, Schmidmaier G, Grützner PA, and Helbig L
- Subjects
- Animals, Antigens, CD metabolism, Antigens, Differentiation, Myelomonocytic metabolism, Bone Regeneration drug effects, Bone Remodeling drug effects, Bone Substitutes pharmacology, Bone Transplantation, Calcium Sulfate administration & dosage, Calcium Sulfate pharmacology, Combined Modality Therapy, Drug Combinations, Durapatite administration & dosage, Durapatite pharmacology, Femoral Fractures therapy, Gentamicins administration & dosage, Gentamicins pharmacology, Lipopolysaccharide Receptors metabolism, Rats, Rats, Sprague-Dawley, Bone Substitutes administration & dosage, Fractures, Ununited therapy, Neovascularization, Physiologic drug effects, Parathyroid Hormone administration & dosage
- Abstract
Non-unions continue to present a challenge to trauma surgeons, as current treatment options are limited, duration of treatment is long, and the outcome often unsatisfactory. Additionally, standard treatment with autologous bone grafts is associated with comorbidity at the donor site. Therefore, alternatives to autologous bone grafts and further therapeutic strategies to improve on the outcome and reduce cost for care providers are desirable. In this study in Sprague-Dawley rats we employed a recently established sequential defect model, which provides a platform to test new potential therapeutic strategies on non-unions while gaining mechanistic insight into their actions. The effects of a combinatorial treatment of a bone graft substitute (HACaS+G) implantation and systemic PTH administration was assessed by µ-CT, histological analysis, and bio-mechanical testing and compared to monotreatment and controls. Although neither PTH alone nor the combination of a bone graft substitute and PTH led to the formation of a stable union, our data demonstrate a clear osteoinductive and osteoconductive effect of the bone graft substitute. Additionally, PTH administration was shown to induce vascularization, both as a single adjuvant treatment and in combination with the bone graft substitute. Thus, systemic PTH administration is a potential synergistic co-treatment to bone graft substitutes.
- Published
- 2021
- Full Text
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69. Evaluation of the AMP SARS-CoV-2 rapid antigen test in a hospital setting.
- Author
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Leixner G, Voill-Glaninger A, Bonner E, Kreil A, Zadnikar R, and Viveiros A
- Subjects
- Adenosine Monophosphate, Hospitals, Humans, Real-Time Polymerase Chain Reaction, Retrospective Studies, Sensitivity and Specificity, COVID-19, SARS-CoV-2
- Abstract
Objectives: Quick and inexpensive SARS-CoV-2 screening and frontline testing are in growing demand. Our study aimed to evaluate the performance of the immunochromatographic AMP rapid antigen test (AMP RAT) compared to the gold-standard real-time reverse transcription PCR (rRT-PCR) in a hospital cohort., Methods: A total of 392 patients, who presented consecutively with COVID-19 symptoms in our emergency department, were included in this retrospective study. Two swabs were collected per patient: a nasopharyngeal for the RAT and a combined naso- and oropharyngeal for the rRT-PCR. A positive rRT-PCR (defined as cycle threshold (Ct) < 40) was found in 94 (24%) patients., Results: In our cohort with a median patient age of 70, overall sensitivity and specificity of the AMP RAT was 69.2% (58.8-78.3, 95% CI) and 99.7% (98.1-100.0, 95% CI), respectively. In patients with a Ct value < 25 and < 30, higher sensitivities of 100.0% (89.4-100.0, 95% CI) and 91.8% (81.9-97.3%, 95% CI) were observed., Conclusions: The AMP RAT showed a high sensitivity in patients with a Ct value < 25 and < 30 and might be helpful for frontline testing whenever rRT-PCR is not readily available., (Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
70. Mitochondrial Enzymes of the Urea Cycle Cluster at the Inner Mitochondrial Membrane.
- Author
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Haskins N, Bhuvanendran S, Anselmi C, Gams A, Kanholm T, Kocher KM, LoTempio J, Krohmaly KI, Sohai D, Stearrett N, Bonner E, Tuchman M, Morizono H, Jaiswal JK, and Caldovic L
- Abstract
Mitochondrial enzymes involved in energy transformation are organized into multiprotein complexes that channel the reaction intermediates for efficient ATP production. Three of the mammalian urea cycle enzymes: N-acetylglutamate synthase (NAGS), carbamylphosphate synthetase 1 (CPS1), and ornithine transcarbamylase (OTC) reside in the mitochondria. Urea cycle is required to convert ammonia into urea and protect the brain from ammonia toxicity. Urea cycle intermediates are tightly channeled in and out of mitochondria, indicating that efficient activity of these enzymes relies upon their coordinated interaction with each other, perhaps in a cluster. This view is supported by mutations in surface residues of the urea cycle proteins that impair ureagenesis in the patients, but do not affect protein stability or catalytic activity. We find the NAGS, CPS1, and OTC proteins in liver mitochondria can associate with the inner mitochondrial membrane (IMM) and can be co-immunoprecipitated. Our in-silico analysis of vertebrate NAGS proteins, the least abundant of the urea cycle enzymes, identified a protein-protein interaction region present only in the mammalian NAGS protein-"variable segment," which mediates the interaction of NAGS with CPS1. Use of super resolution microscopy showed that NAGS, CPS1 and OTC are organized into clusters in the hepatocyte mitochondria. These results indicate that mitochondrial urea cycle proteins cluster, instead of functioning either independently or in a rigid multienzyme complex., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Haskins, Bhuvanendran, Anselmi, Gams, Kanholm, Kocher, LoTempio, Krohmaly, Sohai, Stearrett, Bonner, Tuchman, Morizono, Jaiswal and Caldovic.)
- Published
- 2021
- Full Text
- View/download PDF
71. Overlapping pathogenic signalling pathways and biomarkers in preeclampsia and cardiovascular disease.
- Author
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Suvakov S, Bonner E, Nikolic V, Jerotic D, Simic TP, Garovic VD, Lopez-Campos G, and McClements L
- Subjects
- Blood Pressure, Computational Biology, Databases, Genetic, Female, Heart Failure diagnosis, Heart Failure metabolism, Heart Failure physiopathology, Humans, Hypertension diagnosis, Hypertension metabolism, Hypertension physiopathology, Pre-Eclampsia diagnosis, Pre-Eclampsia metabolism, Pre-Eclampsia physiopathology, Pregnancy, Stroke Volume, Ventricular Function, Left, Gene Regulatory Networks, Heart Failure genetics, Hypertension genetics, Pre-Eclampsia genetics, Signal Transduction genetics
- Abstract
Objectives: Preeclampsia is a cardiovascular pregnancy complication that occurs in 5-10% of pregnancies and it can lead to a number of pregnancy complications including maternal and foetal death. Long-term, preeclampsia is associated with up to 8-fold increased risk of cardiovascular disease (CVD) for both mothers and their offspring. The lack of mechanistic data in relation to the causes or consequences of preeclampsia has prevented the development of effective therapeutic and monitoring strategies., Study Design: This study investigates common underlying mechanisms of preeclampsia and CVD, specifically hypertension and heart failure with preserved ejection fraction (HFpEF), using "in silico" approach of publicly available datasets. Integrated techniques were designed to mine data repositories and identify relevant biomarkers associated with these three conditions., Main Outcomes Measures: The knowledge base tools were employed that enabled the analysis of these biomarkers to discover potential molecular and biological links between these three conditions., Results: Our bioinformatics "in silico" analyses of the publically available datasets identified 76 common biomarkers between preeclampsia, hypertension and HFpEF. These biomarkers were representative of 29 pathways commonly enriched across the three conditions which were largely related to inflammation, metabolism, angiogenesis, remodelling, haemostasis, apoptosis and the renin-angiotensin-aldosterone (RAAS) system., Conclusions: This bioinformatics approach uses the wealth of scientific data available in public repositories to gain a deeper understanding of the overlapping pathogenic mechanisms of associated diseases, which could be explored as biomarkers or targets to prevent long-term cardiovascular complications such as hypertension and HFpEF following preeclampsia., (Copyright © 2020 International Society for the Study of Hypertension in Pregnancy. Published by Elsevier B.V. All rights reserved.)
- Published
- 2020
- Full Text
- View/download PDF
72. Workshop on Recent Issues in Bioanalysis (WRIB) Poster Award winners 2019.
- Author
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Darling R, Bonner E, and Li P
- Subjects
- Humans, Awards and Prizes, Biological Assay methods
- Abstract
The 13th WRIB was held in New Orleans, LA, USA in April 2019. It drew over 1000 professionals representing large pharmas, biotechs, contract research organizations and multiple regulatory agencies from around the world. The Global Bioanalytical Community convened in New Orleans to discuss current topics of interest in bioanalysis, biomarkers, immunogenicity and gene therapy. High quality, better compliance to regulations and scientific excellence are always the foundations of this workshop. Bioanalysis and Bioanalysis Zone are very proud to be supporting the WRIB Poster Awards again this year, and we feature the profiles of the authors of the winning posters. Visit www.bioanalysis-zone.com to see the winning posters in full.
- Published
- 2019
- Full Text
- View/download PDF
73. An Integrative Biomedical Informatics Approach to Elucidate the Similarities Between Pre-Eclampsia and Hypertension.
- Author
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Lopez-Campos G, Bonner E, and McClements L
- Subjects
- Female, Humans, Pregnancy, Hypertension, Pre-Eclampsia
- Abstract
Pre-eclampsia is a pregnancy condition affecting 5-10% of pregnancies, and it is the leading cause of death in pregnancy associated with increased risk of cardiovascular disease later in life. Despite research, the pathogenesis of pre-eclampsia is still poorly understood. In this paper, we investigate the overlapping pathogenic mechanisms between pre-eclampsia and adult hypertension using an integrative biomedical informatics strategy that combined text mining techniques to identify genes and proteins, with geneset analyses, generating knowledge on the pathways and mechanisms involved in these conditions. We identified several overlapping pathogenic pathways/systems including metabolic pathways, developmental biology pathways, immune system, haemostasis, tyrosine kinase pathways, extracellular matrix and oxidative stress pathways. This bioinformatics approach could be applied for investigating mechanistic pathways of other disorders.
- Published
- 2019
- Full Text
- View/download PDF
74. Enterococcus faecalis bacteremia and mitral valve endocarditis under dabigatran for stroke prevention.
- Author
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Stöllberger C, Bonner E, and Finsterer J
- Subjects
- Aged, Bacteremia diagnosis, Dabigatran, Endocarditis diagnosis, Fatal Outcome, Female, Gram-Positive Bacterial Infections diagnosis, Heart Valve Diseases chemically induced, Heart Valve Diseases diagnosis, Heart Valve Diseases microbiology, Humans, Mitral Valve drug effects, Mitral Valve microbiology, Stroke diagnosis, beta-Alanine adverse effects, Bacteremia chemically induced, Benzimidazoles adverse effects, Endocarditis chemically induced, Enterococcus faecalis isolation & purification, Gram-Positive Bacterial Infections chemically induced, Stroke drug therapy, beta-Alanine analogs & derivatives
- Published
- 2014
- Full Text
- View/download PDF
75. Cortical surface mapping using topology correction, partial flattening and 3D shape context-based non-rigid registration for use in quantifying atrophy in Alzheimer's disease.
- Author
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Acosta O, Fripp J, Doré V, Bourgeat P, Favreau JM, Chételat G, Rueda A, Villemagne VL, Szoeke C, Ames D, Ellis KA, Martins RN, Masters CL, Rowe CC, Bonner E, Gris F, Xiao D, Raniga P, Barra V, and Salvado O
- Subjects
- Adult, Aged, Algorithms, Anatomy, Cross-Sectional, Atrophy, Biomarkers, Cognitive Dysfunction pathology, Female, Functional Laterality physiology, Humans, Imaging, Three-Dimensional, Middle Aged, Neurodegenerative Diseases pathology, Reproducibility of Results, Software, Alzheimer Disease pathology, Brain Mapping methods, Cerebral Cortex pathology, Image Processing, Computer-Assisted methods, Magnetic Resonance Imaging methods
- Abstract
Magnetic resonance (MR) provides a non-invasive way to investigate changes in the brain resulting from aging or neurodegenerative disorders such as Alzheimer's disease (AD). Performing accurate analysis for population studies is challenging because of the interindividual anatomical variability. A large set of tools is found to perform studies of brain anatomy and population analysis (FreeSurfer, SPM, FSL). In this paper we present a newly developed surface-based processing pipeline (MILXCTE) that allows accurate vertex-wise statistical comparisons of brain modifications, such as cortical thickness (CTE). The brain is first segmented into the three main tissues: white matter, gray matter and cerebrospinal fluid, after CTE is computed, a topology corrected mesh is generated. Partial inflation and non-rigid registration of cortical surfaces to a common space using shape context are then performed. Each of the steps was firstly validated using MR images from the OASIS database. We then applied the pipeline to a sample of individuals randomly selected from the AIBL study on AD and compared with FreeSurfer. For a population of 50 individuals we found correlation of cortical thickness in all the regions of the brain (average r=0.62 left and r=0.64 right hemispheres). We finally computed changes in atrophy in 32 AD patients and 81 healthy elderly individuals. Significant differences were found in regions known to be affected in AD. We demonstrated the validity of the method for use in clinical studies which provides an alternative to well established techniques to compare different imaging biomarkers for the study of neurodegenerative diseases., (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Published
- 2012
- Full Text
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76. Sex education in medicine - implications for family life education.
- Author
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Bonner EJ and Gendel ES
- Published
- 2011
- Full Text
- View/download PDF
77. Left ventricular hypertrabeculation/noncompaction associated with coronary heart disease and myopathy.
- Author
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Finsterer J, Stöllberger C, and Bonner E
- Subjects
- Aged, Cardiomyopathies complications, Cardiomyopathies pathology, Coronary Disease complications, Coronary Disease pathology, Humans, Isolated Noncompaction of the Ventricular Myocardium complications, Isolated Noncompaction of the Ventricular Myocardium pathology, Male, Ultrasonography, Cardiomyopathies diagnostic imaging, Coronary Disease diagnostic imaging, Isolated Noncompaction of the Ventricular Myocardium diagnostic imaging
- Abstract
Objectives: The association of left ventricular hypertrabeculation (LVHT), also known as noncompaction, coronary heart disease, and metabolic myopathy, as presented in the following report, is rare., Case Report: In a 77-yo male with a history of arterial hypertension, coronary heart disease, dilative cardiomyopathy, mitral and tricuspid insufficiency, AV-block III, implantation of a pacemaker, atrial fibrillation, and heart failure, LVHT was detected on transthoracic echocardiography during hospitalization for worsening heart failure. Clinical neurologic investigation, revealing bilateral ptosis, madarosis, absent eyelashes, bilateral hypacusis, sore neck muscles, generally absent deep tendon reflexes, weakness for foot extension, and ataxic stance, and recurrently elevated creatine-kinase with normal troponine, suggested a metabolic myopathy. Autopsy after death from intractable heart failure. 17 months later confirmed severe coronary heart disease and LVHT in the apex., Conclusions: LVHT may be associated with coronary heart disease and myopathy and may be exclusively located in the left ventricular apex., (Copyright © 2009 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2011
- Full Text
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78. Acquired noncompaction associated with coronary heart disease and myopathy.
- Author
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Finsterer J, Stöllberger C, and Bonner E
- Subjects
- Aged, Echocardiography, Fatal Outcome, Humans, Male, Myocardium pathology, Ventricular Dysfunction, Left etiology, Coronary Artery Disease complications, Muscular Diseases complications, Ventricular Dysfunction, Left diagnostic imaging
- Abstract
In a 77-year-old man with a history of arterial hypertension, coronary heart disease, dilative cardiomyopathy, mitral and tricuspid insufficiency, arteriovenous block III, implantation of a pacemaker, atrial fibrillation, and heart failure, left ventricular hypertrabeculation (LVHT) was detected on transthoracic echocardiography during hospitalization for worsening heart failure. Revision of previous echocardiography did not show LVHT in any of the previous investigations why LVHT was interpreted as acquired. The additional presentation with bilateral ptosis, madarosis (absent eyelashes), bilateral hypoacusis, sore neck muscles, absent tendon reflexes, weakness for foot extension, ataxic stance, and recurrently elevated creatine kinase with normal troponin-T suggested a metabolic myopathy. Autopsy after death resulting from intractable heart failure, 17 months later, confirmed severe coronary heart disease and LVHT in the apex. The case confirms that LVHT may be acquired in single cases with neuromuscular disease and may represent an adaptive mechanism of an impaired myocardium., (Copyright 2010 Elsevier Inc. All rights reserved.)
- Published
- 2010
- Full Text
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79. Mutual associations between malignancy, age, gender, and subsite incidence of colorectal cancer.
- Author
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Brozek W, Kriwanek S, Bonner E, Peterlik M, and Cross HS
- Subjects
- Female, Humans, Male, Age Factors, Colorectal Neoplasms epidemiology, Sex Factors
- Abstract
Background: A cross-sectional study was performed on a cohort of colorectal cancer (CRC) patients to reveal any influence of age, gender, and subsite on grades of malignancy., Patients and Methods: Data from histopathological grading according to WHO criteria were pooled into groups of low-grade (well and moderately differentiated) and high-grade (poorly and undifferentiated) cancer and analyzed for associations., Results: In general, women with CRC were significantly older than men (p<0.05). In particular, women with high-grade cancer in the proximal and distal colon had a median age of 75 years and were thus 10-15 years older (p<0.01 and p<0.05, respectively) than their male counterparts. In contrast, high-grade rectal cancer developed in both genders around the early age of 60 years., Conclusion: Women are protected from more aggressive cancer in the colon though not in the rectum until well after menopause. This likely reflects the differential sensitivity of the mucosa at these sites against the anticancer effects triggered by activation of estrogen receptor-beta.
- Published
- 2009
80. Correlated downregulation of estrogen receptor beta and the circadian clock gene Per1 in human colorectal cancer.
- Author
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Mostafaie N, Kállay E, Sauerzapf E, Bonner E, Kriwanek S, Cross HS, Huber KR, and Krugluger W
- Subjects
- Circadian Rhythm, Female, Humans, Male, Period Circadian Proteins, RNA, Messenger genetics, Reverse Transcriptase Polymerase Chain Reaction, Colorectal Neoplasms genetics, Down-Regulation, Estrogen Receptor beta genetics, Intracellular Signaling Peptides and Proteins genetics
- Abstract
There is a growing body of evidence that disturbed circadian clock gene expression is associated with tumor development and tumor progression. Based on our initial experiments demonstrating decreased period 1 (Per1) expression in colon cancer, we evaluated clock gene and estrogen receptor (ER) alpha/beta expression in colon cancer cells of primary colorectal tumors and adjacent normal colon mucosa (NM) by real-time RT-PCR. Analysis of gene expression in G(2) and G(3) colorectal tumors revealed a decrease of Per1 mRNA compared with paired NM (G(2): 0.52-fold; P = n.s. and G(3): 0.48-fold; P = 0.03). A significant gender specific difference of Per1 expression was observed in G(2) tumors as compared with NM (female: 0.38-fold; P = 0.004 vs. male: 0.73-fold; P = n.s.). Expression of CLOCK was significantly elevated in G(2) tumors of male patients (1.63-fold, P = 0.01). The expression of ER-beta was significantly decreased in G(2) and G(3) tumors (G(2): 0.32-fold; P = 0.003 and 0.27; P = 0.001). No significant gender specific differences of ER-beta reduction in tumors were observed. A significant correlation between the decrease of Per1 and ER-beta in colorectal tumors (r = 0.61; P < 0.001) was found. No changes in gene expression were detected for ER-alpha and Per2. Our data demonstrate a correlated decrease of Per1 and ER-beta in colorectal tumors, mediated probably by epigenetic mechanisms. The observed gender differences in the expression of CLOCK and Per1 in G(2) tumors might suggest a gender-specific, distinctive role of the cellular clock in colorectal tumorigenesis.
- Published
- 2009
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81. Automatic delineation of sulci and improved partial volume classification for accurate 3D voxel-based cortical thickness estimation from MR.
- Author
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Acosta O, Bourgeat P, Fripp J, Bonner E, Ourselin S, and Salvado O
- Subjects
- Aged, Algorithms, Female, Humans, Image Enhancement methods, Male, Reproducibility of Results, Sensitivity and Specificity, Alzheimer Disease pathology, Artificial Intelligence, Cerebral Cortex pathology, Image Interpretation, Computer-Assisted methods, Imaging, Three-Dimensional methods, Magnetic Resonance Imaging methods, Pattern Recognition, Automated methods
- Abstract
Accurate cortical thickness estimation in-vivo is important for the study of many neurodegenerative diseases. When using magnetic resonance images (MRI), accuracy may be hampered by artifacts such as partial volume (PV) as the cortex spans only a few voxels. In zones of opposed sulcal banks (tight sulci) the measurement can be even more difficult. The aim of this work is to propose a voxel-based cortical thickness estimation method from MR by integrating a mechanism for correcting sulci delineation after an improved partial volume classification. First, an efficient and accurate framework was developed to enhance partial volume classification with structural information. Then, the correction of sulci delineation is performed after a homotopic thinning of a cost function image. Integrated to our voxel-based cortical thickness estimation pipeline, the overall method showed a better estimate of thickness and a high reproducibility on real data (R2 > 0.9). A quantitative analysis on clinical data from an Alzheimer's disease study showed significant differences between normal controls and Alzheimer's disease patients.
- Published
- 2008
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82. Current treatment and clinical trial developments for ductal carcinoma in situ of the breast.
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Boughey JC, Gonzalez RJ, Bonner E, and Kuerer HM
- Subjects
- Antibodies, Monoclonal pharmacology, Antibodies, Monoclonal, Humanized, Antineoplastic Agents pharmacology, Biopsy, Cell Proliferation, Female, Humans, Medical Oncology methods, Research Design, Risk, Time Factors, Trastuzumab, Treatment Outcome, Breast Neoplasms therapy, Carcinoma, Intraductal, Noninfiltrating therapy, Clinical Trials as Topic methods
- Abstract
Ductal carcinoma in situ (DCIS) is the fastest growing subtype of breast cancer, mainly because of the aging of our populations and improvements in diagnostic mammography and core biopsy. DCIS represents a proliferation of malignant-appearing cells that have not invaded beyond the ductal basement membrane and is a precursor for the development of invasive breast cancer (IBC). Approximately 40% of patients with DCIS treated with biopsy alone, without complete excision or further therapy, develop IBC. Most DCIS itself is harmless if it is detected and excised before it can progress to IBC, and the current approach to DCIS treatment is aimed at just that goal. Typically, it consists of multimodal treatment including segmental mastectomy followed by radiation therapy to the whole breast and then hormonal therapy or total mastectomy followed by hormonal therapy. This review discusses the state-of-the-art in DCIS detection and treatment and highlights promising new strategies in the care of DCIS patients. The data regarding the effectiveness of breast-conserving surgery versus total mastectomy, the possible avoidance of radiation therapy in some subgroups of patients, and the role of hormonal agents are reviewed. Neoadjuvant therapy and the use of trastuzumab for DCIS are currently under investigation and may be future treatment options for DCIS.
- Published
- 2007
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83. Regulation of genes of the circadian clock in human colon cancer: reduced period-1 and dihydropyrimidine dehydrogenase transcription correlates in high-grade tumors.
- Author
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Krugluger W, Brandstaetter A, Kállay E, Schueller J, Krexner E, Kriwanek S, Bonner E, and Cross HS
- Subjects
- CLOCK Proteins, Colonic Neoplasms enzymology, Colonic Neoplasms metabolism, Dihydrouracil Dehydrogenase (NADP) biosynthesis, Dihydrouracil Dehydrogenase (NADP) genetics, Eye Proteins biosynthesis, Female, Humans, Male, Period Circadian Proteins, RNA, Messenger biosynthesis, RNA, Messenger genetics, Reverse Transcriptase Polymerase Chain Reaction, Trans-Activators biosynthesis, Trans-Activators genetics, Transcription, Genetic, Circadian Rhythm genetics, Colonic Neoplasms genetics, Eye Proteins genetics, Gene Expression Regulation, Neoplastic
- Abstract
Expression of dihydropyrimidine dehydrogenase (DPD) displays a regular daily oscillation in nonmalignant cells. In colorectal cancer cells, the expression of this 5-fluorouracil-metabolizing enzyme is decreased, but the reason remains unclear. In this study, we analyzed by real-time reverse transcription-PCR (RT-PCR) the expression of DPD and of members of the cellular oscillation machinery, period 1 (Per1), period 2 (Per2), and CLOCK, in primary colorectal tumors and normal colon mucosa derived from the same patients. Analysis of tumors according to differentiation grade revealed a 0.46-fold (P = 0.005) decrease for DPD mRNA and a 0.49-fold (P = 0.004) decrease for Per1 mRNA in undifferentiated (G3) tumors compared with paired normal mucosa. In this tumor cohort, the correlation between DPD and Per1 levels was r = 0.64, P < 0.01. In moderately differentiated (G2) colon carcinomas, reduction of DPD and Per1 mRNA levels did not reach significance, but a significant correlation between the respective mRNA levels was detectable (r = 0.54; P < 0.05). The decrease and correlation of DPD and Per1 mRNA levels were even more pronounced in female (G3) patients (DPD: female, 0.35-fold, P < 0.001 versus male, 0.58-fold, P < 0.05; and Per1: female, 0.47-fold, P < 0.01 versus male, 0.52-fold, P < 0.01). The highly significant correlation of DPD mRNA with Per1 mRNA expression suggests control of DPD transcription by the endogenous cellular clock, which is more pronounced in women. Our results also revealed a disturbed transcription of Per1 during tumor progression, which might be the cause for disrupted daily oscillation of DPD in undifferentiated colon carcinoma cells.
- Published
- 2007
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84. 25-hydroxyvitamin D3-1alpha-hydroxylase expression in normal and malignant human colon.
- Author
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Bises G, Kállay E, Weiland T, Wrba F, Wenzl E, Bonner E, Kriwanek S, Obrist P, and Cross HS
- Subjects
- 25-Hydroxyvitamin D3 1-alpha-Hydroxylase genetics, Adult, Aged, Aged, 80 and over, Colonic Neoplasms pathology, Fluorescent Antibody Technique, Humans, Immunoblotting, Intestinal Mucosa metabolism, Intestinal Mucosa pathology, Middle Aged, Polymerase Chain Reaction, RNA, Messenger biosynthesis, 25-Hydroxyvitamin D3 1-alpha-Hydroxylase biosynthesis, Colonic Neoplasms metabolism
- Abstract
1,25-dihydroxyvitamin D(3) has anti-mitotic, pro-differentiating, and pro-apoptotic activity in tumor cells. We demonstrated that the secosteroid can be synthesized and degraded not only in the kidney but also extrarenally in intestinal cells. Evaluation of 1,25-dihydroxyvitamin D(3)-synthesizing CYP27B1 hydroxylase mRNA (real-time PCR) and protein (immunoblotting, immunofluorescence) showed enhanced expression in high- to medium-differentiated human colon tumors compared with tumor-adjacent normal mucosa or with colon mucosa from non-cancer patients. In high-grade undifferentiated tumor areas expression was lost. Many cells co-expressed CYP27B1 and the vitamin D receptor. We suggest that autocrine/paracrine antimitotic activity of 1,25-dihydroxyvitamin D(3) could prevent intestinal tumor formation and progression.
- Published
- 2004
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- View/download PDF
85. Left atrial appendage morphology: comparison of transesophageal images and postmortem casts.
- Author
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Stöllberger C, Ernst G, Bonner E, Finsterer J, and Slany J
- Subjects
- Acrylic Resins, Aged, Aged, 80 and over, Atrial Appendage pathology, Diagnosis, Differential, Endocarditis, Bacterial diagnostic imaging, Endocarditis, Bacterial pathology, Female, Heart Atria diagnostic imaging, Heart Atria pathology, Humans, Image Interpretation, Computer-Assisted, Male, Mathematical Computing, Middle Aged, Reference Values, Sensitivity and Specificity, Shock, Cardiogenic diagnostic imaging, Shock, Cardiogenic pathology, Thrombosis diagnostic imaging, Thrombosis pathology, Atrial Appendage diagnostic imaging, Echocardiography, Transesophageal
- Abstract
Background: Aim of the study was to compare 1) transesophageal echocardiographic (TEE) measurements of the left atrial appendage (LAA) with postmortem casts and 2) the TEE with the postmortem diagnosis of LAA thrombi., Methods: From the TEE images and LAA casts length, orifice, diameter and number of branches were assessed. LAA area was measured by TEE and LAA volume from the cast., Results: In 12 patients who underwent TEE and autopsy, measurements of LAA length and area correlated well with the cast volume ( r=0.6 to r=0.93). The agreement between TEE and LAA casts, concerning the number of branches, was only moderate. In one patient, a false positive diagnosis of a LAA thrombus occurred., Conclusions: LAA size and orifice diameter can be assessed reliably by TEE. The complex LAA morphology hampers measurements of LAA length, branches, course and diagnosis of thrombi.
- Published
- 2003
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- View/download PDF
86. Molecular and functional characterization of the extracellular calcium-sensing receptor in human colon cancer cells.
- Author
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Kállay E, Bonner E, Wrba F, Thakker RV, Peterlik M, and Cross HS
- Subjects
- Blotting, Southern, Caco-2 Cells, Cell Division, DNA, Neoplasm metabolism, Disease Progression, Humans, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction, Colonic Neoplasms genetics, Colonic Neoplasms metabolism, Gene Expression Regulation, Neoplastic, Mutation, Receptors, Calcium-Sensing genetics, Receptors, Calcium-Sensing metabolism
- Abstract
Presence of a functional extracellular calcium-sensing receptor (CaR) is of particular relevance for the growth-inhibitory action of Ca2+ on human colon carcinoma cells. In order to detect CaR gene alterations that may have occurred during the tumorigenic process, we applied Southern blot, DNA sequence, and RT-PCR analysis to DNA from normal human colon mucosa and from cancerous lesions of different grading, as well as from primary cultured and established colonic carcinoma cell lines (e.g., Caco-2). No evidence was obtained for mutations or other sequence alterations in the CaR gene in any of the colon carcinoma cells analyzed. Only a differential expression of two splice variants of the CaR gene, which are generated by usage of different promoters in the 5'-untranslated region, was detected in colon carcinomas of different grade. From Western blot analysis a tendency towards lower CaR protein levels in carcinoma cells in parallel with tumor progression became apparent. Activation of the CaR by extracellular Ca2+ or by specific receptor agonists resulted in substantial growth inhibition in Caco-2 cells. Activation of the CaR was transduced into inhibition of phospholipase A2-mediated arachidonic acid formation, but also into increased production of cAMP and IP3. This provides evidence for a cell type-specific function of the CaR in human colonocytes. We conclude that neoplastic colon epithelial cells can respond to antimitogenic signals generated by activation of the CaR as long as they express sufficient amounts of the CaR protein. This provides a rationale for the use of calcium in chemoprevention of colon tumor development.
- Published
- 2003
- Full Text
- View/download PDF
87. Reduction in the minimum candidate interval in the dominant-intermediate form of Charcot-Marie-Tooth neuropathy to D19S586 to D19S432.
- Author
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Speer MC, Graham FL, Bonner E, Collier K, Stajich JM, Gaskell PC, Pericak-Vance MA, and Vance JM
- Subjects
- Female, Genes, Dominant, Genetic Heterogeneity, Humans, Lod Score, Male, Pedigree, Charcot-Marie-Tooth Disease genetics, Genetic Markers
- Abstract
As part of an on-going genomic screen of unlinked Charcot-Marie-Tooth disease type 2 (CMT2) families, we identified 11 regions in the genome with lod scores > or = 1.0. One of these regions was near the recently identified CMTDI1 locus on 19q. We show evidence of linkage of DUK 1118 to this region and our data reduce the minimum candidate interval for CMTDI1 to the 9-cM interval spanned by D19S586 and D19S432. We also demonstrate that five additional CMT2 families are unlinked to 19q markers, providing further evidence of CMT2 heterogeneity.
- Published
- 2002
- Full Text
- View/download PDF
88. Studies with His475Tyr glutamate carboxipeptidase II polymorphism and neural tube defects.
- Author
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Vieira AR, Trembath D, Vandyke DC, Murray JC, Marker S, Lerner G, Bonner E, and Speer M
- Subjects
- Case-Control Studies, Female, Genetic Predisposition to Disease genetics, Genetic Testing, Genotype, Glutamate Carboxypeptidase II, Humans, Male, Neural Tube Defects epidemiology, Antigens, Surface, Carboxypeptidases genetics, Mutation genetics, Neural Tube Defects genetics, Polymorphism, Genetic
- Published
- 2002
- Full Text
- View/download PDF
89. Fine mapping and genetic heterogeneity in the pure form of autosomal dominant familial spastic paraplegia.
- Author
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Ashley-Koch A, Bonner ER, Gaskell PC, West SG, Tim R, Wolpert CM, Jones R, Farrell CD, Nance M, Svenson IK, Marchuk DA, Boustany RM, Vance JM, Scott WK, and Pericak-Vance MA
- Subjects
- Chromosomes, Human, Pair 19, Chromosomes, Human, Pair 2, Chromosomes, Human, Pair 8, Female, Genes, Dominant, Genetic Linkage, Genetic Markers, Genotype, Haplotypes, Humans, Lod Score, Male, Pedigree, Chromosome Mapping, Chromosomes, Human, Pair 12, Spastic Paraplegia, Hereditary genetics
- Abstract
We evaluated seven families segregating pure, autosomal dominant familial spastic paraplegia (SPG) for linkage to four recently identified SPG loci on chromosomes 2q (1), 8q (2), 12q (3), and 19q (4). These families were previously shown to be unlinked to SPG loci on chromosomes 2p, 14q, and 15q. Two families demonstrated linkage to the new loci. One family (family 3) showed significant evidence for linkage to chromosome 12q, peaking at D12S1691 (maximum lod = 3.22). Haplotype analysis of family 3 did not identify any recombinants among affected individuals in the 12q candidate region. Family 5 yielded a peak lod score of 2.02 at marker D19S868 and excluded linkage to other known SPG loci. Haplotype analysis of family 5 revealed several cross-overs in affected individuals, thereby potentially narrowing the SPG12 candidate region to a 5-cM region between markers D19S868 and D19S220. Three of the families definitively excluded all four loci examined, providing evidence for further genetic heterogeneity of pure, autosomal dominant SPG. In conclusion, these data confirm the presence of SPG10 (chromosome 12), potentially reduce the minimum candidate region for SPG12 (chromosome 19q), and suggest there is at least one additional autosomal dominant SPG locus.
- Published
- 2001
- Full Text
- View/download PDF
90. 25-Hydroxyvitamin D(3)-1alpha-hydroxylase and vitamin D receptor gene expression in human colonic mucosa is elevated during early cancerogenesis.
- Author
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Cross HS, Bareis P, Hofer H, Bischof MG, Bajna E, Kriwanek S, Bonner E, and Peterlik M
- Subjects
- Adenocarcinoma etiology, Adenocarcinoma metabolism, Adenocarcinoma pathology, Blotting, Western, Cell Transformation, Neoplastic metabolism, Cholestanetriol 26-Monooxygenase, Colorectal Neoplasms etiology, Colorectal Neoplasms pathology, Gene Expression, Histocytochemistry, Humans, Intestinal Mucosa pathology, RNA, Messenger metabolism, Receptors, Calcitriol metabolism, Reverse Transcriptase Polymerase Chain Reaction, Up-Regulation, Colorectal Neoplasms metabolism, Intestinal Mucosa chemistry, Receptors, Calcitriol genetics, Steroid Hydroxylases genetics
- Abstract
Human colorectal cancer cells not only express the nuclear vitamin D receptor (VDR) but are also endowed with 25-hydroxy-vitamin D(3)-1alpha-hydroxylase activity and therefore are able to produce the specific ligand for the VDR, the hormonally active steroid 1alpha,25-dihydroxyvitamin D(3) (1alpha,25(OH)(2)D(3)). In the present study we show by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) as well as by Western blotting and immunohistochemical methods, that in human large intestinal carcinomas expression of the genes encoding the 25-(OH)D(3)-1alpha-hydroxylase as well as the VDR increases in parallel with ongoing dedifferentiation in the early phase of cancerogenesis, whereas in poorly differentiated late stage carcinomas only low levels of the respective mRNAs can be detected. This indicates that, through up-regulation of this intrinsic 1alpha,25(OH)(2)D(3)/VDR system which mediates the anti-mitotic effects of the steroid hormone, colorectal cancer cells are apparently able to increase their potential for an autocrine counter-regulatory response to neoplastic cell growth, particularly in the early stages of malignancy.
- Published
- 2001
- Full Text
- View/download PDF
91. Cost-effectiveness of a post-exposure HIV chemoprophylaxis program for blood exposures in health care workers.
- Author
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Marin MG, Van Lieu J, Yee A, Bonner E, and Glied S
- Subjects
- Adult, Anti-HIV Agents economics, Cost-Benefit Analysis, Decision Trees, Drug Therapy, Combination, Humans, Middle Aged, Models, Econometric, New Jersey, Quality-Adjusted Life Years, Zidovudine economics, Anti-HIV Agents therapeutic use, HIV Infections prevention & control, Health Care Costs, Infectious Disease Transmission, Patient-to-Professional prevention & control, Zidovudine therapeutic use
- Abstract
We performed a cost-effectiveness analysis of a post-exposure chemoprophylaxis program for health care workers who sustained exposures to blood. We analyzed a program of (1) treatment with zidovudine alone versus no treatment and (2) treatment with three-drug therapy versus no treatment. Assuming that 35% of exposures were to HIV-positive sources, the zidovudine regimen prevented 53 HIV seroconversions per 100,000 exposures, at a societal cost of $2.0 million per case of HIV prevented. The cost per quality-adjusted life year saved was $175,222. A three-drug chemoprophylactic therapy program (postulating 100% effectiveness and 35% source HIV positivity), prevented 66 seroconversions per 100,000 exposures, at a cost of $2.1 million per case of HIV prevented and $190,392 per quality-adjusted life year saved. Treating sources known to be HIV-positive and treating severe exposures were the most cost-effective strategies.
- Published
- 1999
- Full Text
- View/download PDF
92. The Enterococcus faecalis pyr operon is regulated by autogenous transcriptional attenuation at a single site in the 5' leader.
- Author
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Ghim SY, Kim CC, Bonner ER, D'Elia JN, Grabner GK, and Switzer RL
- Subjects
- Amino Acid Sequence, Base Sequence, Gene Expression Regulation, Bacterial, Genes, Bacterial, Molecular Sequence Data, Multigene Family, Nucleic Acid Conformation, Pentosyltransferases biosynthesis, Recombinant Proteins biosynthesis, Repressor Proteins biosynthesis, Terminator Regions, Genetic, Transcription, Genetic, Uracil pharmacology, 5' Untranslated Regions, Bacterial Proteins, Enterococcus faecalis genetics, Operon, Pentosyltransferases genetics, Pyrimidine Nucleotides biosynthesis, Repressor Proteins genetics
- Abstract
The 5' end of the Enterococcus faecalis pyr operon specifies, in order, the promoter, a 5' untranslated leader, the pyrR gene encoding the regulatory protein for the operon, a 39-nucleotide (nt) intercistronic region, the pyrP gene encoding a uracil permease, a 13-nt intercistronic region, and the pyrB gene encoding aspartate transcarbamylase. The 5' leader RNA is capable of forming stem-loop structures involved in attenuation control of the operon. No attenuation regions, such as those found in the Bacillus subtilis pyr operon, are present in the pyrR-pyrP or pyrP-pyrB intercistronic regions. Several lines of evidence demonstrate that the E. faecalis pyr operon is repressed by uracil via transcriptional attenuation at the single 5' leader termination site and that attenuation is mediated by the PyrR protein.
- Published
- 1999
- Full Text
- View/download PDF
93. The effects of gamma-oryzanol supplementation during resistance exercise training.
- Author
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Fry AC, Bonner E, Lewis DL, Johnson RL, Stone MH, and Kraemer WJ
- Subjects
- Adult, Blood Pressure drug effects, Calcium blood, Cholesterol blood, Cholesterol, HDL blood, Heart Rate drug effects, Hormones blood, Humans, Hydrocortisone blood, Magnesium blood, Male, Serum Albumin metabolism, Testosterone blood, Triglycerides blood, Exercise physiology, Phenylpropionates administration & dosage, Weight Lifting
- Abstract
To determine the effectiveness of gamma-oryzanol supplementation, weight-trained males were randomly divided into supplemented (G-O) and control placebo (Con) groups. The G-O group ingested 500 mg.day-1 of gamma-oryzanol according to manufacturer's instructions. Test batteries were administered before (T1), after 4 weeks (T2), and after 9 weeks (T3) of a periodized resistance exercise program. Both groups demonstrated significant increases in 1 repetition maximum muscular strength (bench press and squat) and vertical jump power, with no differences between the groups. No differences between groups were observed for measures of circulating concentrations of hormones (testosterone, cortisol, estradiol, growth hormone, insulin, beta-endorphin), minerals (calcium, magnesium), binding protein (albumin), or blood lipids (total cholesterol, triglycerides, HDL-cholesterol). Resting cardiovascular variables decreased similarly for both groups. These data suggest that 9 weeks of 500 mg.day-1 of gamma-oryzanol supplementation does not influence performance or related physiological parameters in moderately weight-trained males.
- Published
- 1997
- Full Text
- View/download PDF
94. Morphology of the left atrial appendage.
- Author
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Ernst G, Stöllberger C, Abzieher F, Veit-Dirscherl W, Bonner E, Bibus B, Schneider B, and Slany J
- Subjects
- Adult, Aged, Aged, 80 and over, Corrosion Casting, Echocardiography, Transesophageal, Female, Heart Atria pathology, Heart Diseases diagnostic imaging, Heart Septal Defects, Atrial pathology, Humans, Hypertrophy, Left Ventricular pathology, Male, Middle Aged, Thrombosis diagnosis, Thrombosis pathology, Heart Atria anatomy & histology, Heart Diseases pathology
- Abstract
Background: When examining the left atrial appendage by transesophageal echocardiography, differences in size and shape of the left atrial appendage are to be observed. The study was carried out with the aim of investigating the morphology of the left atrial appendage and to find associations with pathologic cardiac findings., Methods and Results: In 220 cases (106 female, 114 male, mean age 72 +/- 13 years) a cast of the left atrial appendage was made after the post mortem examination by using synthetic resin. In 198 cases an ECG was available (sinus rhythm n = 143, atrial fibrillation n = 55). The casts were described in respect to course and ramifications of the principal axis. The casts were measured concerning orifice diameters, outline, and volume. Most frequently (42%) the course of the principal axis was angulated below 100 degrees. More than five ramifications of the principal axis were found in 56% of the casts. The volume ranged from 770-19,270 mm3 (mean 5,220 +/- 3,041). When comparing the clinical and autopsy-data of the patients with the morphology of the casts, associations could be found between the volume of the casts and atrial fibrillation (7,060 mm3 as compared to 4,645 mm3 in sinus rhythm, P < 0.01), left ventricular hypertrophy (5,740 mm3 as compared to 4,639 mm3 without hypertrophy, P < 0.01), myocardial scars (5,923 mm3 as compared to 4,891 mm3 without scars, P < 0.05), closed foramen ovale (5,515 mm3 as compared to 4,037 mm3 with patent foramen ovale, P < 0.01), and left atrial appendage thrombi (8,566 mm3 as compared to 5,027 mm3 without thrombi, P < 0.01)., Conclusion: Left atrial appendages are formations greatly varying in volume and shape. This variability should be considered when interpreting images of the left atrial appendage, and in particular when diagnosing thrombi.
- Published
- 1995
- Full Text
- View/download PDF
95. Perspectives on medical service at an urban HMO.
- Author
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Axelrod SJ, Bentley WG, Bonner EJ, and Rangarajan NS
- Subjects
- Michigan, Patient Compliance, Patient Education as Topic, Health, Health Maintenance Organizations organization & administration, Urban Health
- Published
- 1980
96. For the new graduate--purchasing a practice.
- Author
-
Bonner E
- Subjects
- Costs and Cost Analysis, Financial Management, Partnership Practice, Dental economics, Practice Management, Dental economics, Professional Practice organization & administration
- Published
- 1983
97. Effect of methotrexate and 5-fluorodeoxyuridine on ribonucleotide reductase activity in mammalian cells.
- Author
-
Elford HL, Bonner EL, Kerr BH, Hanna SD, and Smulson M
- Subjects
- Animals, DNA biosynthesis, Enzyme Induction drug effects, HeLa Cells drug effects, Liver drug effects, Liver Regeneration, Male, Protein Biosynthesis, Rats, Thymine Nucleotides metabolism, Floxuridine pharmacology, HeLa Cells enzymology, Liver enzymology, Methotrexate pharmacology, Ribonucleotide Reductases biosynthesis
- Abstract
A number of studies in bacteria have indicated that deoxythymidine 5'-triphosphate may be a repressor or corepressor of ribonucleotide reductase. For determination of whether a similar regulating mechanism exists in mammalian cells, HeLa cells and partially hepatectomized rats were treated with either methotrexate, 5-fluorouracil, or 5-fluorodeoxyuridine in order to block thymidylate synthesis and consequently lower the intracellular pools of deoxythymidine 5'-triphosphate. In HeLa cells there was a significant (360 to 400 percent) increase in reductase activity in both the methotrexate and 5-fluorodeoxyuridine-treated cells. The administration of methotrexate to partially hepatectomized rats resulted in a 2.7-fold enhancement of the hepatectomy-induced increase in reductase activity, and the 5-fluorouracil treatment yielded a 60 percent increment in the increase of ribonucleotide reductase activity after partial hepatectomy. Cycloheximide prevented the increase in reductase activity after the exposure of HeLa cells to methotrexate and 5-fluorodeoxyuridine, indicating that the stimulation of ribonucleotide reductase activity was the result of enhancement of de novo enzyme synthesis rather than of enzyme activation. The data support the thesis that deoxythymidine 5'-triphosphate or a thymidylate metabolite may be involved in the regulation of ribonucleotide reductase levels in mammalian cells.
- Published
- 1977
98. Lipoid nephrosis appearing as acute oliguric renal failure.
- Author
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Hulter HN and Bonner EL Jr
- Subjects
- Acute Disease, Aged, Biopsy, Needle, Diagnosis, Differential, Glomerular Filtration Rate, Glucocorticoids therapeutic use, Humans, Kidney pathology, Male, Oliguria drug therapy, Proteinuria diagnosis, Sodium urine, Uremia drug therapy, Acute Kidney Injury diagnosis, Anuria diagnosis, Nephrosis, Lipoid diagnosis, Oliguria diagnosis
- Abstract
Acute oliguric renal failure previously was reported to develop in patients with preexisting idiopathic nephrotic syndrome in association with clinical evidence of vascular volume depletion. We describe an 81-year-old man without recent proteinuria or evidence of preexisting nephrotic syndrome in whom acute oliguric renal failure developed. Renal biopsy disclosed minimal change disease. Nephrotic range proteinuria without severe hypoalbuminemia was detected during the 25-day course of oliguric renal failure. Renal vein thrombosis was excluded. Urine sodium concentration and fractional sodium excretion were reduced, yet left ventricular filling pressure was not subnormal and could be increased to supernormal levels without improvement in glomerular filtration rate. Oliguria and azotemia were corrected following initiation of glucocorticoid therapy. This case suggests that lipoid nephrosis can appear as acute oliguric renal failure without historical or physical evidence of preexisting nephrotic syndrome.
- Published
- 1980
99. The need for and value of a maxillofacial prosthodontic service in the Witwatersrand-Vaal area: Part II: A survey of patients who required maxillofacial prosthodontic treatment.
- Author
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Bonner EM, Wolfaardt JF, and Cleaton-Jones P
- Subjects
- Age Factors, Aged, Aged, 80 and over, Health Planning, Humans, Middle Aged, Patient Care Team, South Africa, Health Services Needs and Demand, Health Services Research, Maxillofacial Prosthesis
- Published
- 1986
100. Renal and systemic acid-base effects of the chronic administration of hypercalcemia-producing agents: calcitriol, PTH, and intravenous calcium.
- Author
-
Hulter HN, Sebastian A, Toto RD, Bonner EL Jr, and Ilnicki LP
- Subjects
- Acids urine, Animals, Bicarbonates blood, Clodronic Acid pharmacology, Dogs, Female, Hypercalcemia blood, Hypercalcemia chemically induced, Parathyroid Glands surgery, Thyroidectomy, Acid-Base Equilibrium drug effects, Calcitriol pharmacology, Calcium pharmacology, Hypercalcemia urine, Parathyroid Hormone pharmacology
- Published
- 1982
- Full Text
- View/download PDF
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